EP1480914A1

EP1480914A1 - Method for preventing the uncontrolled growth of microorganisms in water

Info

Publication number: EP1480914A1
Application number: EP03743296A
Authority: EP
Inventors: David Beddie; Sally-Ann Painter; Tobias Wirth
Original assignee: Betatec Hopfenprodukte GmbH
Current assignee: Betatec Hopfenprodukte GmbH
Priority date: 2002-03-07
Filing date: 2003-03-07
Publication date: 2004-12-01
Also published as: WO2003074431A1; AU2003227017A1; DE10209934A1; US20050145565A1

Abstract

The invention relates to a method for controlling biological processes, particularly digestion processes in water, particularly in stagnant or flowing water systems, whereby a certain quantity of tree resins is added to the water.

Description

DESCRIPTION

Driving for the control of biological processes in water

The invention relates to a ner driving to control biological processes, in particular digestion processes in water, with the further features of the preamble of claim 1.

From WO 01/88205 AI a process for the production of sugar or sugar-containing products from sugar-containing vegetable raw materials is known, which is carried out at least partially in the presence of added natural food-compatible resins. These food compatible resins can be tree resins, especially rosin or rosin derivatives. These tree resins are used to inhibit special microorganisms.

Both in standing and flowing water systems there is always the risk of growth of microorganisms such as. B. bacteria, fungi, algae and the like, which leads to a considerable reduction in the quality of the water. This is problematic e.g. B. in flowing water systems in the food sector, the water flow z. B. is used to transport the food.

Chemical means are known to avoid the uncontrolled growth of microorganisms, but they are associated with considerable negative side effects. Such chemical substances are usually also toxic to humans and animals. Therefore, natural substances are preferred that have the required antimicrobial properties.

The present invention is based on the object of providing a method for controlling biological processes in water which is environmentally friendly, compatible with humans, simple to carry out and inexpensive.

This object is achieved by the entire teaching of claims 1 and 12. Advantageous further developments of the respective method result from the Subclaims 2 - 1 l and 20 - 22 or 13 - 22.

An advantageous composition for controlling biological processes in water is taught in claims 24-26 and 27-28.

In the method according to the invention for controlling biological processes, in particular digestion processes in water, in particular in standing and flowing water systems, a certain amount of tree resins is added to the water. Tree resins can control the growth of microorganisms and / or macroorganisms in water. In this context, the word "control" includes the inhibition of growth, the destruction and the removal of microorganisms and / or macroorganisms. The biological processes are controlled by means of the tree resins in a purely natural way. Tree resins are also easy to store and can be stored in without problems The method according to the invention can be used in a wide variety of areas, for example in the food industry, for example in brewery pasteurization; air purification systems; in the oil industry; fountains; water ears, water tanks, waste water systems, Wastewater treatment etc.

Rosin and / or rosin derivatives can be used with particular advantage as tree resins. It is precisely these resins that have proven themselves in the process for controlling biological processes in water and have proven to be particularly advantageous.

In order to ensure effective control of the biological processes in the water, the tree resins, in particular rosin and / or rosin derivatives, are added to the water in an amount of approximately 0.01 mg / 1 to 10000 mg / 1, preferably 0.1 mg / 1 up to 1000 mg / 1, in particular 1 to 100 mg / 1 added.

The tree resins can be used as saline solutions or suspensions, preferably as potassium salt solution, in particular as 0.5 to 35% potassium salt solution or as sodium salt solution, in particular as 0.5 to 35% sodium salt solution. Alternatively, the tree resins can also be used as other 5 to 20% saline solutions or suspensions can be added.

It is also possible for the tree resins to be added as an alcoholic solution or suspension, preferably as a 1 to 25%, in particular as a 10 to 80%, ethanol solution.

Advantageously, the tree resins or their saline or alcoholic solutions or suspensions can be stabilized before they are added to the water. For this purpose, they can be mixed with at least one stabilizing agent, in particular with a surfactant. Such a stabilizer prevents or prevents precipitation or flocculation of the tree resins before they are added to the water. The stabilizer also aids in the distribution of the active ingredients once added to the water. The stabilization rank is particularly advantageous in the case of longer storage of large amounts of tree resins or rosin and / or rosin derivatives in order to always ensure the same control properties of the biological processes in water.

The stabilizing agent can be selected from the group consisting of dioctyl sodium sulfosuccinate, sodium monomethymaphthalene sulfonate and dimethylnaphthalene sulfonate, N-lauroylsarcosine sodium salt, alkyl polyglycoside and

Sodium dodecyldiphenyloxide disulfonate can be removed.

It is also particularly advantageous to add at least one biocide to the water in an effective amount. It can be z. B. act food-compatible, antimicrobial agents (z. B. hops or hop derivatives). The biocides also include agents that prevent the growth or emergence of macroorganisms such. B. prevent mussels or the like. In particular, the biocide can be selected from a group comprising <5-chloro-2-methyl-4-isothiazolin-3-one, 2-methyl-4-isothiazolin-3-one, glutaraldehyde, 2,2-dibromo-3-nitrilopropionamide, 2 -Brom-2-nitropropan-l, 3-diol, 1-bromo-1 - (bromomethyl) - 1, 3-propanedicarbonitrile, tetrachloroisophthalonitrile,

Alkyldimethylbenzylammonium chloride, dimethyldialkylammonium chloride,

Poly (oxyethylene (dimethyliminio) ethylene (dimethyliminio) ethylene dichloride, methylene bisthiocyanate, 2-decylthioethanamine, tetrakishydroxymethylphosphonium sulfate, Dithiocarbamate, cyanodithioimidocarbonate, 2-methyl-5-nitroimidazole-1-ethanol, 2- (2-bromo-2-nitroethenyl) furan, beta-bromo-beta-nitrostyrene, beta-nitrostyrene, beta-nitrovinylfuran, 2-bromo-2 -bromomethylglutaronitrile, bis (trichloromethyl) sulfone, S- (2-hydroxypropyl) thiomethanesulfonate, tetrahydro-3,5-dimethyl-2H-l, 3,5-hydrazine-2-thione, 2- (thiocyanomethylthio) benzothiazole, 2-bromo -4'-hydroxyacetophenone, 1,4-

Bis (bromoacetoxy) -2-butene, bis (tributyltin) oxide, copper sulfate, (2-tert-butylamino) -4-chloro-6- (ethylamino) -s-triazine, dodecylguanidine acetate, dodecylguanidine hydrochloride, cocoalkyldimethylamine oxide, n- Kokosalkyltrimethylendiamin,

Tetraalkylphosphonium chloride, 7-oxabicyclo [2.2. l] heptane-2,3-dicarboxylic acid, 2- (4-thiazolyl) benzimidazole, orthophenylphenol, 6-ethoxy-l, 2-dihydro-2,2,4-trimethylquinoline and 4,5-dichloro-2-n-octyl -4-isothiazolin-3-one can be selected.

In order to ensure an effective effect of the biocide, it can be added to the water in an amount of 0.01 to 10000 mg / l, preferably 0.05 to 50 mg / 1, in particular 0.1 to 10 mg / 1.

It is possible to add a combination of tree resins (especially rosin and / or rosin derivatives) with biocides to the water, as well as to add the two components tree resins and biocides separately to the water.

Depending on the requirements, the proportion of tree resins can be approximately 0.1% to 99%, and in the opposite way the proportion of biocides can also be approximately 0.1% to 99%.

As already explained above, a certain amount of stabilized tree resins can be added to the water to control biological processes. This feature can be seen separately from the independent claim 12. The optional features described above can also be implemented.

The tree resins or the stabilized tree resins can be added to the water in dry or pasty or viscous form. The state of matter can also depend on the stabilizing agent or the saline or alcoholic solutions or suspensions, or can thus be deliberately influenced. The tree resins or stabilized tree resins can expediently be added to the water at a temperature of -5 ° C. to 95 ° C. Within this temperature range it is also possible that organisms can grow if there is no control of the biological processes.

With a further advantage, the tree resins or stabilized tree resins can be used in combination with hops or hop derivatives, which are distinguished by their antimicrobial properties.

Hop acid, in particular beta acid, can advantageously be added to the water. A mixture of resin acids, in particular tree resin acids and beta acids, which are added to the water in particular in a 50:50 ratio, leads to the control of biological processes, in particular the inhibition of growth, the destruction and also the removal of microorganisms and / or macroorganisms is optimized.

The following example is intended to prevent algae growth by resin acids, especially tree resin acids, e.g. B. Rosin acids and the synergy effect with beta acids illustrate:

First, a pure culture of Chlorella vulgaris fo. viridis obtained from CCAP (culture accumulation of algae and protozoa). This culture was then grown in a fresh and sterile proteose medium. 20 ml portions of this substance were filled into glass tubes and different concentrations of resin acids and / or beta acids were added. After nine days, the chlorophyll pigments were extracted and measured. If we speak of resin acids in the following, these are in particular tree resin acids, advantageously rosin acids.

Data analysis was performed using Jeffrey 's and Humphrey's trichromatic equations. The chlorophyll content of a sample was determined after extraction by measuring OD at 750, 664, 647 and 630nm. The value at 750nm was subtracted from the absorption values at 664, 647 and 630nm. The Concentrations (mg / 1) in the extracted solution of chlorophyll a, b and et + c were calculated by inserting the 750 nm corrected absorption values into the following equations:

C _{E> a} = 11.85 (Paragraph 664) - 1.54 (Paragraph 647) - 0.08 (Paragraph 630) C _{E) b} = 21.03 (Paragraph 647) - 5.43 (Paragraph 664) - 2.66 (Paragraph 630) C _{E) c} = 24.52 (Section 630) - 7.6 (Section 647) - 1.67 (Section 664)

In which:

C _{E, a} = concentration (mg / 1) of chlorophyll a, which was analyzed in the extracted solution. C _{E, b} ⁼ concentration (mg / 1) of chlorophyll b that was analyzed in the extracted solution.

C _E , _C = concentration (mg / 1) of chlorophyll C] + c ₂ , which was analyzed in the extracted solution.

Results:

Table 1 shows the amount and type of chlorophyll contained in the cultures of Chlorella vulgaris after their treatment with different concentrations of resin and beta acids after an incubation period of nine days. The results show that 100 ppm of the resin acids significantly reduced the chlorophyll content of a sample, which is a clear indication of an algae-inhibiting activity.

A mixture of 50ppm resin acids and 50ppm beta acids reduced the chlorophyll content of a sample more than when separately adding 100ppm each of the biocides. This shows the synergy effect between the two components resin acid and beta acid. Table 1

The following example shows the prevention of gram-positive bacteria by resin acids:

Two species of the bacterial species Bacillus stearothermophilus DSM 22 and DSM 457, a gram-positive bacterial species, were grown on a culture medium at 55 ° C. which contained known concentrations of resin acids. The percentage growth of the bacteria was measured in comparison to a control substance without any resin acids.

The results show that the concentrations of resin acids in a range of 1 to 5 ppm have a significant influence on the prevention / inhibition of the growth of the two types of bacteria. The following table shows the result of this experiment:

Independent patent claim 23 shows the advantageous use of tree resins in the control of biological processes in water, in particular digestion processes in standing or flowing water systems.

An advantageous composition for controlling biological processes in water is described in independent claims 24 and 27. According to claim 24, the composition contains a certain amount of tree resins. Rosin and / or rosin derivatives can be provided as tree resins. Further possible characteristics of the composition result from the method described above.

According to claim 27, the composition contains a certain amount of stabilized tree resins. With regard to the optional features, reference is also made to the method described above.

Claims

1. Methods for the control of biological processes, in particular digestion processes in water, in particular in standing or flowing water systems,

characterized in that

a certain amount of tree resins is added to the water.

2. Procedure according to speech 1,

characterized in that

rosin and / or rosin derivatives are used as tree resins.

3. The method according to any one of claims 1 or 2,

characterized in that

the tree resins are added to the water in an amount of 0.01 to 10000 mg / 1, preferably 0.1 to 1000 mg / 1, in particular 1 to 100 mg / 1.

4. The method according to any one of the preceding claims,

characterized in that

the tree resins are used as saline solutions or suspensions, preferably as potassium salt solution, in particular as 0.5 to 35% potassium salt solution or as sodium salt solution, in particular as 0.5 to 35% Sodium chloride solution.

5. The method according to any one of the preceding claims,

characterized in that

the tree resins are added as 5 to 20% saline solutions or suspensions.

6. The method according to any one of the preceding claims,

characterized in that

the tree resins are added as an alcoholic solution or suspension, preferably as a 1 to 95%, in particular as a 10 to 80% ethanol solution.

7. The method according to any one of the preceding claims,

characterized in that

the tree resins or their saline or alcoholic solutions or suspensions with at least one prior to their addition to the water

Stabilizing agents, especially a surfactant, are added.

8. The method according to the preceding speech,

characterized in that the Grappe stabilizing agent comprising dioctyl sodium sulfosuccinate, sodium monomethyl naphthalene sulfonate and dimethyl naphthalene sulfonate, N-lauroylsarcosine sodium salt, alkyl polyglycoside and

Sodium dodecyldiphenyloxide disulfonate is selected.

9. The method according to any one of the preceding claims,

characterized in that

at least one effective amount of biocide is added to the water.

10. Method according to speech 9,

characterized in that

the biocide from the group comprising 5-chloro-2-methyl-4-isothiazolin-3-one, 2-methyl-4-isothiazolin-3-one, glutaraldehyde, 2,2-dibromo-3-nitrilopropionamide, 2-bromo 2-nitropropane-1,3-diol, 1-bromo-1 - (bromomethyl) - 1,3-propanedicarbonitrile,

Tefrachloroisophthalonitrile, alkyldimethylbenzylammonium chloride,

Dimethyldialkylammonium chloride, poly (oxyethylene (dimethyliminio) - ethylene (dimethyliminio) ethylene dichloride, methylene bisthiocyanate, 2-

Decylthioethanamine, tetrakishydroxymethylphosphonium sulfate, dithiocarbamate, cyanodithioimidocarbonate, 2-methyl-5-nitroimidazole-1-ethanol, 2- (2-bromo-2-nitroethenyl) furan, beta-bromo-beta-nitrostyrene, beta-nitrostyrene, beta-

Nitrovinylfuran, 2-bromo-2-bromomethylglutaronitrile, bis (trichloromethyl) sulfone, S- (2-hydroxypropyl) thiomethanesulfonate, tetrahydro-3,5-dimethyl-2H-l, 3,5-hydrazine-2-thione, 2- ( Thiocyanomethylthio) benzothiazole, 2-bromo-4'-hydroxyacetophenone, 1,4-bis (bromoacetoxy) -2-butene, bis (tributyltin) oxide, copper sulfate, (2-tert-butylamino) -

4-chloro-6- (ethylamino) -s-triazine, dodecylguanidine acetate, dodecylguanidine hydrochloride, cocoalkyldimethylamine oxide, n-cocoalkyltrimethylene diamine, tetraalkylphosphonium chloride, 7-oxabicyclo [2.2.1] heptane-2,3-dicarboxylic acid, 2- (4- Thiazolyl) benzimidazole, orthophenylphenol, 6-ethoxy-1,2-dihydro-2,2,4-trimethylquinoline and 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one is selected.

11. The method according to any one of claims 9 or 10,

characterized in that

the biocide is added to the water in an amount of 0.01 to 10000 mg / 1, preferably 0.05 to 50 mg / 1, in particular 0.1 to 10 mg / 1.

12. Processes for the control of biological processes, in particular digestion processes in water, in particular in standing or flowing water systems,

characterized in that

a certain amount of stabilized tree resins, in particular stabilized rosin and / or stabilized rosin derivatives, is added to the water.

13. The method according to claim 12,

characterized in that

the tree resins are stabilized with at least one surfactant.

14. The method according to any one of the preceding claims,

characterized in that the Grappe stabilizer comprises dioctyl sodium sulfosuccinate, sodium monomethyl naphthalene sulfonate and dimethyl naphthalene sulfonate, N-lauroylsarcosine sodium salt, alkyl polyglycoside and

Sodium dodecyldiphenyloxide disulfonate is selected.

15. The method according to any one of claims 12 or 13,

characterized in that

the stabilized tree resins are added to the water in an amount of 0.01 to 10000 mg / 1, preferably 0.1 to 1000 mg / 1, in particular 1 to 100 mg / 1.

16. The method according to any one of claims 12-15,

characterized in that

at least one effective amount of biocide is added to the water.

17. The method according to claim 16,

characterized in that

the biocide from the group comprising 5-chloro-2-methyl-4-isothiazolin-3-one, 2-methyl-4-isothiazolin-3-one, glutaraldehyde, 2,2-dibromo-3-nitrilopropionamide, 2-bromo 2-nitropropane-1,3-diol, 1-bromo-1 - (bromomethyl) - 1,3-propanedicarbonitrile, tetrachloroisophthalonitrile, alkyldimethylbenzylammonium chloride,

dimethyldialkylammonium,

Poly (oxyethylene (dimethyliminio) ethylene (dimethyliminio) ethylene dichloride, methylene bisthiocyanate, 2-decylthioethanamine, Tetrakishydroxymethylphosphonium sulfate, dithiocarbamate,

Cyanodithioimidocarbonate, 2-methyl-5-nitroimidazole-1-ethanol, 2- (2-bromo-2-nifroethenyl) furan, beta-bromo-beta-nitrostyrene, beta-nitrostyrene, beta

Nitrovinylfuran, 2-bromo-2-bromomethylglutaronitrile, bis (trichloromethyl) sulfone, S- (2-hydroxypropyl) thiomethanesulfonate, tetrahydro-3, 5-dimethyl-2H- 1, 3, 5-hydrazine-2-thione, 2- ( Thiocyanomethylthio) benzothiazole, 2-bromo-4'-hydroxyacetophenone, 1,4-bis (bromoacetoxy) -2-butene, bis (tributyltin) oxide, copper sulfate, (2-tert-butylamino) - 4-chloro-6- ( ethylamino) -s-triazine, dodecylguanidine acetate, dodecylguanidine hydrochloride, cocoalkyldimethylamine oxide, n-cocoalkyltrimethylene diamine, tetraalkylphosphonium chloride, 7-oxabicyclo [2.2.1] heptane-2,3-dicarboxylic acid, 2- (4-

Thiazolyl) benzimidazole, orthophenylphenol, 6-ethoxy-1,2-dihydro-2,2,4-trimethylquinoline and 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one is selected.

18. The method according to any one of claims 15-16,

characterized in that

19. The method according to any one of claims 12-18,

characterized in that

for the stabilized tree resins at least one of the features of claims 4 to 6 is realized.

20. The method according to any one of the preceding claims,

characterized in that the tree resins or stabilized tree resins are added in dry or pasty or viscous form.

21. The method according to any one of the preceding claims,

characterized in that

the tree resins or stabilized tree resins are added to the water at a temperature of - 5 ° C to 95 ° C.

22. The method according to any one of the preceding claims,

characterized in that

the tree resins or stabilized tree resins are used in combination with hops or hop derivatives.

23. Use of tree resins, in particular rosin and / or rosin derivatives, in the control of biological processes in water, in particular digestion processes in standing or flowing water systems.

24. Composition for the control of biological processes in water, in particular digestion processes in standing and flowing water systems,

characterized in that

the composition contains a certain amount of tree resins.

25. Composition according to address 24, characterized in that

rosin and / or rosin derivatives are provided as tree resins.

26. Composition according to one of claims 24 or 25,

characterized in that

at least one of the features of claims 3 to 22 is realized.

27. Composition for the control of biological processes in water, in particular digestion processes in standing or flowing water systems,

characterized in that

the composition contains a certain amount of stabilized tree resins.

28. The composition according to claim 27,

characterized in that

at least one of the features of claims 3 to 22 is realized.