EP1427852A2 - Single nucleotide amplification and detection by polymerase - Google Patents
Single nucleotide amplification and detection by polymeraseInfo
- Publication number
- EP1427852A2 EP1427852A2 EP02759494A EP02759494A EP1427852A2 EP 1427852 A2 EP1427852 A2 EP 1427852A2 EP 02759494 A EP02759494 A EP 02759494A EP 02759494 A EP02759494 A EP 02759494A EP 1427852 A2 EP1427852 A2 EP 1427852A2
- Authority
- EP
- European Patent Office
- Prior art keywords
- phosphate
- reaction
- dna polymerase
- group
- labeled
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6823—Release of bound markers
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Abstract
Description
Claims
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US31579801P | 2001-08-29 | 2001-08-29 | |
US315798P | 2001-08-29 | ||
US10/113,025 US7033762B2 (en) | 2001-08-29 | 2002-04-01 | Single nucleotide amplification and detection by polymerase |
US113025 | 2002-04-01 | ||
PCT/US2002/027564 WO2003020891A2 (en) | 2001-08-29 | 2002-08-29 | Single nucleotide amplification and detection by polymerase |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP09164344.5 Division-Into | 2009-07-01 |
Publications (3)
Publication Number | Publication Date |
---|---|
EP1427852A2 true EP1427852A2 (en) | 2004-06-16 |
EP1427852A4 EP1427852A4 (en) | 2005-12-28 |
EP1427852B1 EP1427852B1 (en) | 2010-02-17 |
Family
ID=26810636
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP02759494A Expired - Lifetime EP1427852B1 (en) | 2001-08-29 | 2002-08-29 | Single nucleotide amplification and detection by polymerase |
Country Status (9)
Country | Link |
---|---|
US (1) | US7033762B2 (en) |
EP (1) | EP1427852B1 (en) |
JP (1) | JP4360904B2 (en) |
AT (1) | ATE458068T1 (en) |
AU (1) | AU2002324826B2 (en) |
CA (1) | CA2456906C (en) |
DE (1) | DE60235383D1 (en) |
DK (1) | DK1427852T3 (en) |
WO (1) | WO2003020891A2 (en) |
Families Citing this family (110)
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JP4510628B2 (en) * | 2002-08-29 | 2010-07-28 | ジーイー・ヘルスケア・バイオサイエンス・コーポレイション | Terminal phosphate block nucleoside polyphosphate |
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JP4896707B2 (en) * | 2003-02-05 | 2012-03-14 | ジーイー・ヘルスケア・バイオサイエンス・コーポレイション | Solid phase sequencing |
CN101258220A (en) * | 2004-09-16 | 2008-09-03 | 阿普里拉股份有限公司 | Fluorescent dye compounds, conjugates and uses thereof |
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US7805081B2 (en) * | 2005-08-11 | 2010-09-28 | Pacific Biosciences Of California, Inc. | Methods and systems for monitoring multiple optical signals from a single source |
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US7692783B2 (en) * | 2006-02-13 | 2010-04-06 | Pacific Biosciences Of California | Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources |
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US20080076189A1 (en) * | 2006-03-30 | 2008-03-27 | Visigen Biotechnologies, Inc. | Modified surfaces for the detection of biomolecules at the single molecule level |
US8975216B2 (en) * | 2006-03-30 | 2015-03-10 | Pacific Biosciences Of California | Articles having localized molecules disposed thereon and methods of producing same |
US7563574B2 (en) | 2006-03-31 | 2009-07-21 | Pacific Biosciences Of California, Inc. | Methods, systems and compositions for monitoring enzyme activity and applications thereof |
CN101495656B (en) * | 2006-06-07 | 2017-02-08 | 纽约哥伦比亚大学理事会 | DNA sequencing by nanopore using modified nucleotides |
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US8383369B2 (en) * | 2008-09-24 | 2013-02-26 | Pacific Biosciences Of California, Inc. | Intermittent detection during analytical reactions |
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US20230148447A9 (en) | 2008-12-11 | 2023-05-11 | Pacific Biosciences Of California, Inc. | Classification of nucleic acid templates |
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US9175338B2 (en) | 2008-12-11 | 2015-11-03 | Pacific Biosciences Of California, Inc. | Methods for identifying nucleic acid modifications |
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US8518643B2 (en) * | 2010-02-04 | 2013-08-27 | Pacific Biosciences Of California, Inc. | Method to improve single molecule analyses |
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Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1994014972A1 (en) * | 1992-12-23 | 1994-07-07 | Edward David Hyman | Method and apparatus for enzymatic synthesis of oligonucleotides |
WO1994023055A1 (en) * | 1993-03-30 | 1994-10-13 | United States Biochemical Corporation | Cycle sequencing with non-thermostable dna polymerases |
WO2001027153A1 (en) * | 1999-10-13 | 2001-04-19 | Smithkline Beecham Corporation | A murine seven-transmembrane receptor, mus musculus mhneaa81 |
WO2002040126A2 (en) * | 2000-10-02 | 2002-05-23 | Keck Graduate Institute | Methods for identifying nucleotides at defined positions in target nucleic acids using fluorescence polarization |
WO2003020734A2 (en) * | 2001-08-29 | 2003-03-13 | Amersham Biosciences Corp | Labeled nucleoside polyphosphates |
WO2003020984A2 (en) * | 2001-08-29 | 2003-03-13 | Amersham Biosciences Corp | Terminal-phosphate-labeled nucleotides and methods of use |
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US7033762B2 (en) | 2001-08-29 | 2006-04-25 | Amersham Biosciences Corp | Single nucleotide amplification and detection by polymerase |
-
2002
- 2002-04-01 US US10/113,025 patent/US7033762B2/en not_active Expired - Lifetime
- 2002-08-29 DK DK02759494.4T patent/DK1427852T3/en active
- 2002-08-29 DE DE60235383T patent/DE60235383D1/en not_active Expired - Lifetime
- 2002-08-29 JP JP2003525595A patent/JP4360904B2/en not_active Expired - Lifetime
- 2002-08-29 CA CA2456906A patent/CA2456906C/en not_active Expired - Lifetime
- 2002-08-29 AT AT02759494T patent/ATE458068T1/en not_active IP Right Cessation
- 2002-08-29 EP EP02759494A patent/EP1427852B1/en not_active Expired - Lifetime
- 2002-08-29 AU AU2002324826A patent/AU2002324826B2/en not_active Expired
- 2002-08-29 WO PCT/US2002/027564 patent/WO2003020891A2/en active Application Filing
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WO2003020984A2 (en) * | 2001-08-29 | 2003-03-13 | Amersham Biosciences Corp | Terminal-phosphate-labeled nucleotides and methods of use |
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Title |
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ARZUMANOV A A ET AL: "Gamma-Phosphate-substituted 2'-deoxynucleoside 5'-triphosphates as substrates for DNA polymerases" JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOCHEMICAL BIOLOGISTS, BIRMINGHAM,, US, vol. 271, no. 40, 4 October 1996 (1996-10-04), pages 24389-24394, XP002256215 ISSN: 0021-9258 * |
NEWTON C R ET AL: "THE PRODUCTION OF PCR PRODUCTS WITH 5' SINGLE-STRANDED TAILS USING PRIMERS THAT INCORPORATE NOVEL PHOSPHORAMIDITE INTERMEDIATES" NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 21, no. 5, 1993, pages 1155-1162, XP002041613 ISSN: 0305-1048 * |
See also references of WO03020891A2 * |
VASSILIOU W ET AL: "Exploiting Polymerase Promiscuity: A Simple Colorimetric RNA Polymerase Assay" VIROLOGY, ACADEMIC PRESS,ORLANDO, US, vol. 274, no. 2, 1 September 2000 (2000-09-01), pages 429-437, XP004435943 ISSN: 0042-6822 * |
Also Published As
Publication number | Publication date |
---|---|
CA2456906C (en) | 2013-12-10 |
US20030096253A1 (en) | 2003-05-22 |
CA2456906A1 (en) | 2003-03-13 |
AU2002324826B2 (en) | 2007-11-08 |
DK1427852T3 (en) | 2010-06-07 |
EP1427852A4 (en) | 2005-12-28 |
DE60235383D1 (en) | 2010-04-01 |
US7033762B2 (en) | 2006-04-25 |
JP2005501551A (en) | 2005-01-20 |
EP1427852B1 (en) | 2010-02-17 |
WO2003020891A2 (en) | 2003-03-13 |
WO2003020891A3 (en) | 2003-05-08 |
ATE458068T1 (en) | 2010-03-15 |
JP4360904B2 (en) | 2009-11-11 |
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