WO2003020891A2 - Single nucleotide amplification and detection by polymerase - Google Patents
Single nucleotide amplification and detection by polymerase Download PDFInfo
- Publication number
- WO2003020891A2 WO2003020891A2 PCT/US2002/027564 US0227564W WO03020891A2 WO 2003020891 A2 WO2003020891 A2 WO 2003020891A2 US 0227564 W US0227564 W US 0227564W WO 03020891 A2 WO03020891 A2 WO 03020891A2
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- phosphate
- reaction
- dna polymerase
- group
- labeled
- Prior art date
Links
- 0 *C=CC(C(OCC(C=O)OC(c1ccccc1)=O)=*)=* Chemical compound *C=CC(C(OCC(C=O)OC(c1ccccc1)=O)=*)=* 0.000 description 1
- DCAJWNICMGQDLM-UHFFFAOYSA-L [O-]P([O-])(Oc1c[nH]c(cc2)c1c(Cl)c2I)=O Chemical compound [O-]P([O-])(Oc1c[nH]c(cc2)c1c(Cl)c2I)=O DCAJWNICMGQDLM-UHFFFAOYSA-L 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6823—Release of bound markers
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Abstract
Description
Claims
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP02759494A EP1427852B1 (en) | 2001-08-29 | 2002-08-29 | Single nucleotide amplification and detection by polymerase |
JP2003525595A JP4360904B2 (en) | 2001-08-29 | 2002-08-29 | Single nucleotide amplification and polymerase detection |
AU2002324826A AU2002324826B2 (en) | 2001-08-29 | 2002-08-29 | Single nucleotide amplification and detection by polymerase |
AT02759494T ATE458068T1 (en) | 2001-08-29 | 2002-08-29 | SINGLE NUCLEOTIDE AMPLIFICATION AND DETECTION WITH POLYMERASE |
CA2456906A CA2456906C (en) | 2001-08-29 | 2002-08-29 | Single nucleotide amplification and detection by polymerase |
DE60235383T DE60235383D1 (en) | 2001-08-29 | 2002-08-29 | SINGLE NUCLEOTIDE AMPLIFICATION AND DETECTION WITH POLYMERASE |
DK02759494.4T DK1427852T3 (en) | 2001-08-29 | 2002-08-29 | Single nucleotide implication and detection by polymerase |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US31579801P | 2001-08-29 | 2001-08-29 | |
US60/315,798 | 2001-08-29 | ||
US10/113,025 | 2002-04-01 | ||
US10/113,025 US7033762B2 (en) | 2001-08-29 | 2002-04-01 | Single nucleotide amplification and detection by polymerase |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2003020891A2 true WO2003020891A2 (en) | 2003-03-13 |
WO2003020891A3 WO2003020891A3 (en) | 2003-05-08 |
Family
ID=26810636
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2002/027564 WO2003020891A2 (en) | 2001-08-29 | 2002-08-29 | Single nucleotide amplification and detection by polymerase |
Country Status (9)
Country | Link |
---|---|
US (1) | US7033762B2 (en) |
EP (1) | EP1427852B1 (en) |
JP (1) | JP4360904B2 (en) |
AT (1) | ATE458068T1 (en) |
AU (1) | AU2002324826B2 (en) |
CA (1) | CA2456906C (en) |
DE (1) | DE60235383D1 (en) |
DK (1) | DK1427852T3 (en) |
WO (1) | WO2003020891A2 (en) |
Cited By (15)
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WO2003020984A2 (en) * | 2001-08-29 | 2003-03-13 | Amersham Biosciences Corp | Terminal-phosphate-labeled nucleotides and methods of use |
EP1421213A2 (en) * | 2001-08-29 | 2004-05-26 | Amersham Biosciences Corp. | Labeled nucleoside polyphosphates |
EP1546353A2 (en) * | 2002-08-29 | 2005-06-29 | Amersham Biosciences Corp. | Allele specific primer extension |
EP1590479A1 (en) * | 2003-02-05 | 2005-11-02 | Amersham Biosciences Corp. | Nucleic acid amplification |
EP1592779A2 (en) * | 2003-02-05 | 2005-11-09 | Amersham Biosciences Corp. | Terminal-phosphate-labeled nucleotides with new linkers |
US7033762B2 (en) | 2001-08-29 | 2006-04-25 | Amersham Biosciences Corp | Single nucleotide amplification and detection by polymerase |
US7223541B2 (en) | 2001-08-29 | 2007-05-29 | Ge Healthcare Bio-Sciences Corp. | Terminal-phosphate-labeled nucleotides and methods of use |
US7256019B2 (en) | 2001-08-29 | 2007-08-14 | Ge Healthcare Bio-Sciences Corp. | Terminal phosphate blocked nucleoside polyphosphates |
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US7560254B2 (en) | 2001-08-29 | 2009-07-14 | Ge Healthcare Bio-Sciences Corp. | Allele specific primer extension |
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US9885657B2 (en) | 2014-08-08 | 2018-02-06 | Quantum-Si Incorporated | Integrated device with external light source for probing detecting and analyzing molecules |
US9921157B2 (en) | 2014-08-08 | 2018-03-20 | Quantum-Si Incorporated | Optical system and assay chip for probing, detecting and analyzing molecules |
WO2018122157A1 (en) * | 2016-12-28 | 2018-07-05 | Roche Diagnostics Gmbh | Reversibly protected nucleotide reagents with high thermal stability |
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AU2003263023B2 (en) * | 2002-08-29 | 2008-09-11 | Global Life Sciences Solutions Usa Llc | Terminal phosphate blocked nucleoside polyphosphates |
AU2003263025B2 (en) * | 2002-08-29 | 2009-06-18 | Global Life Sciences Solutions Usa Llc | Analyte detection |
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Citations (1)
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US5558991A (en) | 1986-07-02 | 1996-09-24 | E. I. Du Pont De Nemours And Company | DNA sequencing method using acyclonucleoside triphosphates |
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2002
- 2002-04-01 US US10/113,025 patent/US7033762B2/en not_active Expired - Lifetime
- 2002-08-29 DE DE60235383T patent/DE60235383D1/en not_active Expired - Lifetime
- 2002-08-29 JP JP2003525595A patent/JP4360904B2/en not_active Expired - Lifetime
- 2002-08-29 DK DK02759494.4T patent/DK1427852T3/en active
- 2002-08-29 WO PCT/US2002/027564 patent/WO2003020891A2/en active Application Filing
- 2002-08-29 CA CA2456906A patent/CA2456906C/en not_active Expired - Lifetime
- 2002-08-29 EP EP02759494A patent/EP1427852B1/en not_active Expired - Lifetime
- 2002-08-29 AT AT02759494T patent/ATE458068T1/en not_active IP Right Cessation
- 2002-08-29 AU AU2002324826A patent/AU2002324826B2/en not_active Expired
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US5558991A (en) | 1986-07-02 | 1996-09-24 | E. I. Du Pont De Nemours And Company | DNA sequencing method using acyclonucleoside triphosphates |
Non-Patent Citations (2)
Title |
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MARTINEZ, C.I. ET AL., BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, vol. 7, 1997, pages 3013 - 3016 |
MARTINEZ, C.I. ET AL., NUCLEIC ACIDS RESEARCH, vol. 27, 1999, pages 1271 - 1274 |
Cited By (33)
Publication number | Priority date | Publication date | Assignee | Title |
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US7033762B2 (en) | 2001-08-29 | 2006-04-25 | Amersham Biosciences Corp | Single nucleotide amplification and detection by polymerase |
WO2003020984A3 (en) * | 2001-08-29 | 2003-12-11 | Amersham Biosciences Corp | Terminal-phosphate-labeled nucleotides and methods of use |
EP1421213A2 (en) * | 2001-08-29 | 2004-05-26 | Amersham Biosciences Corp. | Labeled nucleoside polyphosphates |
US7560254B2 (en) | 2001-08-29 | 2009-07-14 | Ge Healthcare Bio-Sciences Corp. | Allele specific primer extension |
US7452698B2 (en) | 2001-08-29 | 2008-11-18 | Ge Healthcare Bio-Sciences Corp. | Terminal phosphate blocked nucleoside polyphosphates |
EP1421213A4 (en) * | 2001-08-29 | 2005-12-28 | Amersham Biosciences Corp | Labeled nucleoside polyphosphates |
WO2003020984A2 (en) * | 2001-08-29 | 2003-03-13 | Amersham Biosciences Corp | Terminal-phosphate-labeled nucleotides and methods of use |
US7223541B2 (en) | 2001-08-29 | 2007-05-29 | Ge Healthcare Bio-Sciences Corp. | Terminal-phosphate-labeled nucleotides and methods of use |
US7041812B2 (en) | 2001-08-29 | 2006-05-09 | Amersham Biosciences Corp | Labeled nucleoside polyphosphates |
US7052839B2 (en) | 2001-08-29 | 2006-05-30 | Amersham Biosciences Corp | Terminal-phosphate-labeled nucleotides and methods of use |
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Also Published As
Publication number | Publication date |
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US7033762B2 (en) | 2006-04-25 |
EP1427852B1 (en) | 2010-02-17 |
ATE458068T1 (en) | 2010-03-15 |
DE60235383D1 (en) | 2010-04-01 |
CA2456906C (en) | 2013-12-10 |
DK1427852T3 (en) | 2010-06-07 |
WO2003020891A3 (en) | 2003-05-08 |
CA2456906A1 (en) | 2003-03-13 |
US20030096253A1 (en) | 2003-05-22 |
AU2002324826B2 (en) | 2007-11-08 |
EP1427852A4 (en) | 2005-12-28 |
EP1427852A2 (en) | 2004-06-16 |
JP2005501551A (en) | 2005-01-20 |
JP4360904B2 (en) | 2009-11-11 |
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