EP1367900A2 - Method for controlling harmful organisms in the culture of useful plants - Google Patents

Abstract

The invention relates to a method for controlling harmful organisms in genetically modified in vegetable plants containing a gene derived from Bacillus thuringiensis which codes for an insecticidally effective protein and expresses said protein. The invention is characterized in that an insecticidally effective amount of one or several compounds from the following groups (a) - (f) is applied to plants, the seed products or reproduction products thereof and/or the cultivated area thereof: a) insecticidal organophosphorous compounds b) pyrethroids; c) insecticidal carbamates; d) biopesticides; e) insecticidal growth regulators; f) miscellaneous. The inventive method enables the amount of agricultural pesticides, which act in synergy with the transgenic plants, to be reduced and makes it possible to increase and enhance the efficiency of transgenic plants, offering economic and ecological advantages.

Description

description

Procedure for the control of harmful organisms in crops

The invention relates to a method for controlling harmful organisms in crops of Bt vegetables.

Genetically modified vegetables such as eggplants that express toxins from Bacillus thuringiensis (Bt) and are therefore resistant to attack by certain insect pests (Bt vegetables) are known and are already being used in commercial crop production (see, for example, US Pat. No. 6,072,105). Although such genetically modified vegetables already have very good properties, there are still a number of problems, so that there is a wide room for improvement.

It was therefore still the task to provide the most effective and environmentally compatible problem solutions for controlling vegetable pests.

It has now surprisingly been found that certain classes of insecticides have synergistic effects when used in combination with Bt vegetables.

The invention therefore relates to a method for controlling harmful organisms in genetically modified vegetable plants which contain a gene derived from Bacillus thuringiensis which codes for and expresses an insecticidally active protein, an insecticidally effective amount of one or more compounds from the following being expressed Groups (a) to (f) apply to the plants, their seeds or propagation material and / or their cultivated area:

a) insecticidal organophosphorus compounds from the group: Acephate (4), Azinphos-ethyl (40), Azinphos-methyl (41), Cadusafos (101), Chlorfenvinphos (124), Chlormephos (128), Chlorpyrifos (137), Demeton-S-methyl (205), Diazinon ( 209), Dicrotophos (223), Dimethoate (243), Disulfoton (257), Ethion (283), Ethoprophos (286), Etrimfos (295), Fonofos (366), Isazofos (429), Isofenphos (430), Malathion ( 448), Methamidophos (479), Methidathion (481), Mevinphos (499), Monocrotophos (502), Omethoate (533), Parathion (551), Phenthoate (565), Phorate (570), Phosalone (571), Phosmet ( 572), Phosphamidon (573), Phoxim (575), Pirimiphos-methyl (585), Profenofos (594), Prothiofos (614), Pyridaphenthion (625), Quinalphos (634), Terbufos (690), Tetrachlorvinphos (694) and Triazophos (726);

b) pyrethroids from the group:

Acrinathrin (9), Allethrin (17), Bifenthrin (70), Cycloprothrin (172), Cyfluthrin (176), (Beta) -Cyfluthrin (177), (Lambda) -Cyhalothrin (180), Cypermethrin (183), (Aipha ) - Cypermethrin (184), (Beta) -Cypermethrin (185), (Zeta) -Cypermethrin (187), Deltamethrin (204), Esfenvalerate (277), Fenpropathrin (312), Fenvalerate (319), Flucythrinate (333), (Tau) fluvalinate (362), permethrin (561), tefluthrin (687), tralomethrin (718) and ZXI 8901 (759);

c) insecticidal carbamates from the group:

Alanycarb (15), Aldicarb (16), Amitraz (22), Bendiocarb (56), Benfuracarb (58), Butocarboxim (95), Carbaryl (106), Carbofuran (109), Carbosulfan (110), Ethiofencarb (282), Formetanate (369), Furathiocarb (376), Isoprocarb (431), Methiocarb (482), Methomyl (483), Oxamyl (541), Pirimicarb (583), Propoxur (610), Thiofanox (709), Thiodicarb (708) and Trimethacarb (743); d) Biopesticides from the group:

Bacillus thuringiensis (46, 47), Bacillus firmus, granular and nuclear polyhedron viruses, Beauveria bassiana (52), Beauveria brogniartii (53) and baculoviruses such as Autographa California;

e) insecticidal growth regulators from the group:

Chlorfluazuron (125), DBI-3204, Diflubenzuron (231), Flucycloxuron (332), Flufenoxuron (335), Hexaflumuron (399), Lufenuron (446), Novaluron (527), Methoxyfenozide (643), Teflubenzuron (686) (679) and triflumuron (739);

f) Other:

Abamectin (1), Bensultap (64), Cartap (113), Chlordane (121), Chlorfenapyr (123), DNOC (261), Endosulfan (270), Fipronil (323), Ethiprole, Imidacloprid (418), Thiacloprid, Phosphine (574), oleic acid / fatty acids (532), pymetrozine (615), thiocyclam (707), IKI-220, tolfenpyrad (NEW), acetamiprid (5), spinosad (754), silafluofen (650) and thiamethoxam (NEW).

The numbers in parentheses indicate the registration number in The e-Pesticide Manual ', CD-ROM version 1.1, 1999-2000 (ISBN: 1-901396-22-3), based on The Pesticide Manual', 11th Ed., British Crop Protection Council, Farnham 1997, again.

Baculoviruses are described, for example, in J. Ind. Microbiol. & Biotech (1997) 19: 192. Bacillus firmus, DBI-3204, Thiacloprid and IKI-220 are described, for example, in Proceedings of the BCPC-Conference, Pest & Diseases, 2000. Reference is hereby expressly made to these sources and the literature cited therein, by quotation they count as part of the description.

The process according to the invention enables a reduced application rate of crop protection agents which act synergistically with the transgenic plants, and an increase and broadening of the efficiency of the transgenic plants and thus offers economic and ecological advantages.

The advantages of the method according to the invention lie on the one hand in synergisms with the Bacillus thuringiensis toxins (Bt toxins) produced in the transgenic plant and on the other hand in e.g. reduced number of applications or a reduction in the application rates to partially sublethal doses (compared to the conventional application of the individual insecticides) and a significantly reduced environmental impact associated therewith.

In particular, combinations of the active substances mentioned together with the endogenous, i.e. Within the transgenic plants, Bt toxins produced a pronounced synergistic effect on a large number of harmful organisms to be controlled.

The invention likewise relates to the use of compounds from groups (a) to (f) mentioned above for combating harmful organisms in genetically modified vegetable plants which contain a gene derived from Bacillus thuringiensis which codes for and expresses an insecticidally active protein.

In the context of the invention, the term “insecticidally active” encompasses insecticidal, acaricidal, molluscicidal, nematicidal, ovicidal activity, and also a repellent, behavior-modifying and sterilizing effect.

Preferred insecticidal active ingredients are the insecticides mentioned Organophosphorus compounds, pyrethroids, insecticidal carbamates, insecticidal growth regulators, abamectin, acetamiprid, chlorfenapyr, endosulfan, fipronil, ethiprole, imidacloprid, pymetrozine, spinosad, - silafluofen, thiamethoxam, thiayradopillus, tolurium-220-pyrid, tolurine-220-pyrid, tolurine-220-pyridine

Ethoprophos, malathion, parathion, phosalone, pirimiphos-methyl, triazophos, acrinathrin, deltamethrin, tefluthrin, aldicarb, amitraz, bendiocarb, carbaryl, carbofuran, oxamyl, pirimicarb, thiodicarb, tifluburonuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronuronidifuronidonoxydinoneuronuronidifuronidonoxydononoxinate , Acetamiprid, Chlorfenapyr, Endosulfan, Fipronil, Ethiprole, Imidacloprid, Pymetrozine, Spinosad, Silafluofen, Thiamethoxam, Thiacloprid, Tolfenpyrad, IKI-220 and Bacillus thuringiensis.

Thiodicarb, acetamiprid, fipronil and tolfenpyrad are very particularly preferred.

Mixtures of two or more, preferably two or three, particularly preferably two, of the insecticidally active compounds are also preferred.

Mixtures of the organophosphorus compounds mentioned with the pyrethroids mentioned are particularly preferred.

The mixtures listed below are very particularly preferred: deltamethrin and endosulfan, deltamethrin and spinosad, deltamethrin and chlorphenapyr, deltamethrin and Bacillus thuringiensis, deltamethrin and methoxyfenozide, deltamethrin and tebufenozide, endosulfan and am undisulfinhrishroshrine, endosulfoshrine, endosulfin, and chlorotaphrine, endosulfin, and chlorotaphrine, endosulfin, and chlorosulfur, endosulfan, and chlorosulfur, endosulfan, undisulfur, and chlorosulfur, endosulfan and amitrazillushrin, endosulfin, and chlorosulfur.

Mixtures of the pyrethroids mentioned with imidacloprid and mixtures of the pyrethroids mentioned with tebufenozide are also preferred.

The insecticidally active compounds used according to the invention are known and predominantly commercially available.

The insecticides used according to the invention are usually available as commercial formulations. However, if necessary, they can be formulated in different ways, depending on how it is predetermined by the biological and / or chemical-physical parameters. Examples of possible formulations are:

Wettable powder (WP), emulsifiable concentrates (EC), aqueous solutions (SL), emulsions, sprayable solutions, oil or water-based dispersions (SC), suspoemulsions (SE), dusts (DP), mordants, granules in the form of , Spray, elevator and adsorption granules, water-dispersible granules (WG), ULV formulations, microcapsules, waxes or baits.

These individual formulation types are known in principle and are described, for example, in:

Winnacker-Küchler, 1986, "Chemical Technology", volume 7, 4th edition, C. Hauser Verlag Munich; van Falkenberg, 1972-73, "Pesticides Formulations", 2nd Ed., Marcel Dekker N.Y .; Martens, 1979, "Spray Drying Handbook", 3rd Ed., G. Goodwin Ltd. London.

The necessary formulation auxiliaries, such as inert materials, surfactants, solvents and other additives, are also known and are described, for example, in:

Watkins, "Handbook of Insecticide Dust Diluents and Carriers", 2nd Ed., Darland Books, Caldwell NJ; v. Olphen, "Introduction to Clay Colloid Chemistry", 2nd Ed., J. Wiley & Sons, NY; Marsden, "Solvent Guide," 2nd Ed., Interscience, NY 1950; McCutcheon's, Detergents and Emulsifiers Annual, MC Publ. Corp., Ridgewood, NJ; Sisley and Wood, "Encyclopedia of Surface Active Agents", Chem. Publ. Co. Inc., NY 1964; Schönfeldt, "Interface-active ethylene oxide adducts", Wiss. Publishing company, Stuttgart 1967; Winnacker-Küchler, "Chemical Technology", Volume 7, 4th edition, C. Hanser Verlag Munich 1986.

Based on these formulations, combinations with other pesticidally active substances, fertilizers and / or plant growth regulators can also be produced, e.g. in the form of a finished formulation or as a tank mix. Spray powders are preparations which are uniformly dispersible in water and which, in addition to the active substance, contain wetting agents, e.g. polyoxethylated alkylphenols, polyoxethylated fatty alcohols, alkyl or alkylphenol sulfonates and dispersants, e.g. sodium lignosulfonate, 2,2'-dinaphthylmethane-6,6'-disulfonic acid sodium.

Emulsifiable concentrates are made by dissolving the active ingredient in an organic solvent, e.g. Butanol, cyclohexanone, dimethylformamide, xylene or higher-boiling aromatics or hydrocarbons with the addition of one or more emulsifiers. Examples of emulsifiers which can be used are: alkylarylsulfonic acid calcium salts, such as cadodecylbenzenesulfonate, or nonionic emulsifiers, such as fatty acid polyglycol esters, alkylaryl polyglycol ethers, fatty alcohol polyglycol ethers, propylene oxide-ethylene oxide condensation products, alkyl polyethers, sorbitan fatty acid or fatty acid sorbitol polyesters, fatty oxy-oxy-sorbitol or polyoxyethylene oxy-sorbitol or polyoxyethylene esters or polyoxyethylene sorbitates.

Dusts are obtained by grinding the active ingredient with finely divided solid substances, for example talc, natural clays such as kaolin, bentonite, pyrophillite or diatomaceous earth. Granules can either be produced by spraying the active ingredient onto adsorbable, granulated inert material or by applying active ingredient concentrates by means of adhesives, for example polyvinyl alcohol, sodium polyacrylic acid or mineral oils, to the surface of carriers such as sand, kaolinite or granulated inert material. Can too suitable active ingredients are granulated in the manner customary for the production of fertilizer granules - if desired in a mixture with fertilizers.

The active substance concentration in wettable powders is e.g. about 10 to 90 wt .-%, the rest of 100 wt .-% consists of conventional formulation components. In the case of emulsifiable concentrates, the active substance concentration can be approximately 5 to 80% by weight. Dust-like formulations usually contain 5 to 20 wt .-% of active ingredient, sprayable solutions about 2 to 20 wt .-%. In the case of granules, the active ingredient content depends in part on whether the active compound is in liquid or solid form and which granulation aids, fillers, etc. are used.

In addition, the active ingredient formulations mentioned may contain the customary adhesives, wetting agents, dispersants, emulsifiers, penetrants, solvents, fillers or carriers.

For use, the concentrates present in the commercial form are optionally diluted in a customary manner, e.g. for wettable powders, emulsifiable concentrates, dispersions and sometimes also for microgranules using water. Dust-like and granulated preparations as well as sprayable solutions are usually no longer diluted with other inert substances before use.

With the external conditions such as temperature, humidity, etc. the required application rate varies. It can fluctuate within wide limits, e.g. between 0.1 g / ha and 5.0 kg / ha or more of active substance. However, it is preferably between 0.1 g / ha and 1.0 kg / ha. Because of the synergistic effects between Bt vegetables and insecticide, application rates of 0.1 to 500 g / ha are particularly preferred.

For insecticidal organophosphorus compounds (a), application rates of 50 to 500 g / ha are preferred, and 50 to 200 g / ha are particularly preferred. Application rates of 0.1 to 10 g / ha are preferred for pyrethroids (b), 0.1 to 6.0 g / ha are particularly preferred.

For insecticidal carbamates (c), application rates of 50 to 5000 g / ha are preferred, and 50 to 2000 g / ha are particularly preferred.

For biopesticides (d), the commercial application rates are preferred.

For insecticidal growth regulators (e), application rates of 10 to 1000 g / ha are preferred, 50 to 500 g / ha are particularly preferred.

Application rates of 0.1 to 5000 g / ha are preferred for the insecticides of group (f), 0.1 to 300 g / ha are particularly preferred.

The active compounds according to the invention can be present in their commercially available formulations and in the use forms prepared from these formulations in mixtures with other active compounds, such as insecticides, attractants, sterilants, acaricides, nematicides, fungicides, growth-regulating substances or herbicides.

Preferred further mixing partners are

1. from the group of carboxylic acid esters

Alphametrin, 5-benzyl-3-furylmethyl- (E) -, (1 R) -cis-2,2-di-methyl-3- (2-oxothiolan-3-ylidenemethyl) cyclopropanecarboxylate, bioallethrin, bioallethrin ((S) - cyclopentyl isomer), bioresmethrin, (RS) -1-cyano-1 - (6-phenoxy-2-pyridyl) methyl- (1 RS) -trans-3- (4-tert.butylphenyl) -2,2-dimethylcyclopropane decarboxylate ( NCI 85193), cythithrin, cyphenothrin, empenthrin, fenfluthrin, flumethrin, fluvaiinate (D-isomer), imiprothrin (S-41311), phenothrin ((R) -isomer), prallethrin, pyrethrins (natural products), resmethrin, tetram -Cypermethrin (TD-2344), transfluthrin; 2. from the group of the amidines chlorodime form;

3. Other:

ABG-9008, Anagrapha falcitera, AKD-1022, AKD-3059, Bifenazate (D-2341), Binapacryl, BJL-932, Bromopropylate, BTG-504, BTG-505, Buprofezin, Camphechlor, Chlorobenzilate, 2- (4-chlorophenyl ) -4,5-diphenylthiophene (UBI-T 930), chlororfentezine, chromafenozide (ANS-118), CG-216, CG-217, CG-234, A-184699, cyclopropanecarboxylic acid (2-naphthylmethyl) ester (Ro12- 0470), cyromazine, diaclodes, N- (3,5-dichloro-4- (1, 1, 2,3,3,3-hexafluoro-1-propyloxy) phenyl) carbamoyl) -2-chlorobenzcarboximidic acid ethyl ester, dicofol, N- (2,3-dihydro-3-methyl-1,3-thiazol-2-ylidenes) -2,4-xylidines, dinobutone, dinocap, diofenolan, DPX-062, emamectin benzoates (MK-244), sulfethiprols, ethofenprox , Etoxazole (YI-5301), Fenoxycarb, Fluazuron, Flumite (Flufenzine, SZI-121), 2-Fluoro-5- (4- (4-ethoxyphenyl) -4-methyl-1-pentyl) diphenyl ether (MTI 800), Fenpyroximate, Fenthiocarb, Flubenzimine, Flufenprox (ICI-A5683), Fluproxyfen, Gamma-HCH, Halofenozide (RH-0345), Halofenprox (MTI-732), Hexythiazox, HOI-9004, Hydramethylnon (AC 21730 0), Indoxacarb (DPX-MP062), Kanemite (AKD-2023), M-020, MTI-446, Ivermectin, M- 020, Milbemectin, NC-196, Neemgard, Nitenpyram (TI-304), 2-Nitromethyl- 4,5-dihydro-6H-thiazine (DS 52618), 2-nitromethyl-3,4-dihydrothiazole (SD 35651), 2-nitromethylene-1, 2-thiazinan-3-ylcarbamaldehyde (WL 108477), pyriproxyfen (S- 71639), NC-196, NC-1111, NNI-9768, OK-9701, OK-9601, OK-9602, Propargite, Pyridaben, Pyrimidifen (SU-8801), RH-0345, RYI-210, S-1283, S-1833, SB7242, Sl-8601, Silomadine (CG-177), SU-9118, Tebufenpyrad (MK-239), Tetradifon, Tetrasul, TI-435, Verbutin, Vertalec (Mykotal), YI-5301.

The active substance content of the use forms prepared from the commercially available formulations can be from 0.00000001 to 95% by weight, preferably between 0.00001 and 1% by weight, of active substance. For example, corresponding formulations of mixtures of pyrethroids and organophosphorus compounds contain 0.05 to 0.01% by weight of pyrethroid and 0.25 to 0.20% by weight of organophosphorus compound, preferably 0.01 to 0.001% by weight of pyrethroid and 0, 2 to 0.1% by weight organophosphorus compound.

For mixtures of pyrethroids and endosulfan, a ratio of 0.05 to 0.01% by weight of pyrethroid to 0.7 to 0.2% by weight of endosulfan is preferred, and 0.01 to 0.001% by weight of pyrethroid is particularly preferred and 0.35 to 0.2% by weight endosulfan.

The values given above for pyrethroids apply to mixtures of pyrethroids and Bacillus thuringiensis (Bt), the Bt content is preferably 0.01 to 0.001% by weight, particularly preferably 0.005 to 0.001% by weight.

Mixtures of endosulfan and amitraz preferably contain 0.35 to 0.2% by weight endosulfan and 0.6 to 0.2% by weight amitraz.

The method according to the invention is preferably suitable for use against all developmental stages of the harmful organisms (egg, all larval stages, such as, for example, L1, L2, L3, L4, L5 to Adult), in particular for combating homopters, dipteras, lepidopters and coleopteras.

For the purposes of the invention, the term “Bt vegetables” is to be understood as meaning genetically modified vegetable plants or crops which contain one or more genes from Bacillus thuringiensis, which in turn encode and express insecticidally active proteins. These plants ('Bt vegetables') can be cultivated both outdoors and under the conditions of growing under glass. The corresponding vegetable plants and crops include, for example: Potatoes, preferably

Starch potatoes, sweet potatoes and table potatoes;

Root vegetables, preferably

Carrots (carrots), turnips (table beets, stubble, may beets, autumn turnips, Teltow turnips), salsify, Jerusalem artichoke, parsley root, parsnip, radish and horseradish;

Bulbous vegetables, preferably

Kohlrabi, beetroot, celery (celeriac), radishes and radishes;

Onion vegetables, preferably

Leek, leek and onions (onion and seed onion);

Cabbage vegetables, preferably

Cabbage (white cabbage, red cabbage, leaf cabbage, savoy cabbage), cauliflower, sprouts,

Broccoli, kale, marrow cabbage, sea kale and Brussels sprouts;

Fruit vegetables, preferably

Tomatoes (free-range, shrub, meat, greenhouse, cocktail, industrial and fresh market tomatoes), melons, eggplants, eggplants, peppers (sweet and seasoned peppers, Spanish pepper), hot peppers, pumpkins, zucchini and cucumbers ( Field, greenhouse, snake and pickled cucumbers);

Vegetable legumes, preferably

Bush beans (as broad beans, broad beans, flageolet beans, wax beans, dry cooked beans with green and yellow pods), runner beans (as broad beans, broad beans, flageolet beans, broad beans with green, blue and yellow pods), broad beans (broad beans with broad beans, broad beans, broad beans white and black spotted flowers), peas (flat peas, chickpeas, marker peas, scarf peas, sugar peas, peas, varieties with light and dark green Fresh grain) and lentils;

Leaf and stem vegetables, preferably

Chinese cabbage, lettuce, plucked lettuce, lamb's lettuce, iceberg lettuce, Romaine lettuce, oak leaf lettuce, endive, radicchio, lollo rosso lettuce, rocket lettuce, chicory, spinach, Swiss chard (leaf and stem chard) and parsley;

Other vegetables, preferably

Asparagus, rhubarb, chives, artichokes, mint, sunflowers,

Bulbous fennel, dill, garden cress, mustard, poppy seeds, peanut, sesame and salad chicory.

All plants, which are summarized under ^' the term' Bt vegetables', are genetically modified in the sense of the invention in such a way that they contain and express one or more genes from Bacillus thuringiensis which code for an insecticidally active protein.

Preferred are Bacillus thuringiensis crystal proteins from the Cry family (see e.g. Crickmore et al., 1998, Microbiol. Mol. Biol. Rev. 62: 807-812), which are active against lepidopters, coleopteras and dipteras.

Genes are particularly preferred encoding for the proteins crylAal, cry1Aa2, cry1Aa3, cry1Aa4, cry1Aa5, cry1Aa6, cry1Aa7, crylAaδ, cry1Aa9, cry1Aa10, cry1Aa11 crylAbl, cry1Ab2, cry1Ab3, cry1Ab4, cry1Ab5, cry1Ab6, cry1Ab7, cry1Ab8, cry1Ab9, cry1Ab10, cry1Ab11 , cry1Ab12, cry1Ab13, cry1Ab14, crylAd, cry1Ac2, cry1Ac3, cry1Ac4, cry1Ac5, cry1Ac6, cry1Ac7, crylAcδ, cry1Ayl9, crylAdO, cry1Ac12, cry1Al11, cry1Al9 Bbl, crylBd, crylBdl, cryl Bel, crylCal, cry1Ca2, cry1 Ca3, cry1Ca4, crylCaδ, cry1 Ca6, cry1Ca7, crylCbl, cry1Cb2, cryl Da1, cryl Da2, cryl Db1, Ea1, cryl Ea1, cryl cryl Ea5, cry1 Ea6, cryl Ebl, cryl Fal, cry1 Fa2, cryl Fb1, cryl Fb2, cry1Fb3, cry1 Fb4, crylGal, cry1Ga2, crylGbl, cry1Gb2, crylHal, cryl Hbl, cryllal, cry1la3 cry1la4, cryllaδ, cry1la6, cryllbl, crylld, crylldl, cryllel, cryl l-like, cryUal, cryUbl, cryUcI, crylKal, cryl-like, cry2Aa1, cry2Aa2, cry2Aa3, cry2Aa4, cry2A2 , cry2Ab2, cry2Ab3, cry2Ac1, cry2Ac2, cry2Ad1, cry3Aa1, cry3Aa2, cry3Aa3, cry3Aa4, cry3Aa5, cry3Aa6, cry3Aa7, cry3Ba1, cry3Ba2, cry3Bb1, cry3Bb2, cry3Bb3, cry3Ca1, cry4Aa1, cry4Aa2, cry4Ba1, cry4Ba2, cry4Ba3, cry4Ba4, cry5Aa1 , cry5Ab1, cry5Ac1, cryδBal, cry6Aa1, cry6Ba1, cry7Aa1, cry7Ab1, cry7Ab2, cryδAal, cry8Ba1, cry8Ca1, cry9Aa1, cry9Aa2, cry9Ba1, cry9Ca1, cry9Da1, cry9Da2, cry9Ea1, cry9 like, crylOAal, cry10Aa2, cry11Aa1, cry11Aa2, cry11Ba1, cry11Bb1, cry12Aa1, cry13Aa1, cry14Aa1, cry15Aa1, cry16Aa1, cry17Aa1, cry18Aa1, cry18Ba1, cry18Ca1, cry19Aa1, cry19Ba1, cry20Aa1, cry21Aa1, cry21Aa2, cry22Aa1, cry23Aa1, cry24Aa1, cry25Aa1, cry26Aa1, cry27Aa1, cry28Aa1, cry28Aa2, cry29Aa1, cry30Aa1, cry31Aa1, cytIAal, cyt1Aa2, cyt1Aa3, cyt1Aa4, cytlAbl, cytIBal, cyt2Aa1, cyt2Ba1, c yt2Ba2, cyt2Ba3, cyt2Ba4, cyt2Ba5, cyt2Ba6, cyt2Ba7, cyt2Ba8, cyt2Bb1.

The subfamilies cryl, cry2, cry3, cry5 and cry9 are particularly preferred.

CrylAb, crylAc, cry3A, cry3B and cry9C are very particularly preferred.

It is further preferred to use plants which contain genes for several Bt proteins.

In addition to the expression of toxins from Bacillus thuringiensis (Bt) for insect resistance, the transgenic crop plants can also have other transgenic properties, for example further insect resistance (for example by expression of a protease or peptidase inhibitor, cf. WO-A 95/35031), herbicide resistance (eg against glufosinate or glyphosate by expression of the pat or bar gene) or also nematode, fungal or virus resistance (eg by expression of a glucanase, chitinase) or also be genetically modified in their metabolic properties so that a qualitative and / or quantitative change of ingredients (eg through modifications of the energy, carbohydrate, fatty acid or nitrogen change or metabolite flows influencing this). Bt vegetable plants are preferred, for example, which additionally have a glufosinate or glyphosate resistance.

Bt vegetables, including methods of making them, are described in detail in, for example, Barton et al., 1987, Plant Physiol. 85: 1103-1109; Vaeck et al., 1987, Nature 328: 33-37; Fischhoff et al., 1987, Bio / Technology 5: 807-813.

Bt vegetable plants are still commercially available in various variations, for example the NewLeaf ^® potato variety from Monsanto. Other types of vegetables are under development or testing, such as a tomato variety with resistance to Lepidoptera from Monsanto.

Ways of producing transgenic plants which have modified properties compared to naturally occurring plants exist, for example, in the use of the processes mentioned below: Willmitzer, 1993, Transgenic plants, In: Biotechnology, A Multivolume Comprehensive Treatise, Rehm et al. (eds.), Vol. 2, 627-659, VCH Weinheim, Germany; McCormick et al., 1986, Plant Cell Reports 5: 81-84; EP-A 0221044; EP-A 0131624.

Numerous molecular biological techniques with which new transgenic plants with modified properties can be produced are known to the person skilled in the art; see, e.g., Sambrook et al., 1989, "Molecular Cloning: A Laboratory Manual", 3rd Ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY; Winnacker, 1996, "Genes and Clones", 2nd ed., VCH Weinheim or Christou, 1996, Trends in Plant Science 1: 423-431. For such genetic engineering manipulations, suitable nucleic acid molecules can be introduced into plants or plant cells, for example by means of suitable vectors which allow mutagenesis or a sequence change by recombining DNA sequences. With the help of the standard procedures mentioned above, z. B. base exchanges, partial sequences removed or natural or synthetic sequences added. For example, the naturally occurring genes can also be completely replaced by heterologous or synthetic genes, preferably under the control of a promoter active in plant cells ('gene replacemenf). To connect the DNA fragments to one another, adapters or linkers can be attached to the fragments.

The production of plant cells with a reduced activity of a gene product can be achieved, for example, by the expression of at least one corresponding antisense RNA, a sense RNA to achieve a cosuppression effect or the expression of at least one appropriately constructed ribozyme which specifically cleaves transcripts of the gene product mentioned above.

For this purpose, DNA molecules can be used that comprise the entire coding sequence of a gene product, including any flanking sequences that may be present, as well as DNA molecules that only comprise parts of the coding sequence, these parts having to be long enough to be in the cells to cause an antisense effect. It is also possible to use DNA sequences which have a high degree of homology to the coding sequences of a gene product, but which are not completely identical.

When nucleic acid molecules are expressed in plants, the synthesized protein can be located in any compartment of the plant cell. However, in order to achieve localization in a particular compartment, z. B. the coding region is linked to DNA sequences which ensure localization in a specific compartment or at a specific point in time (at a specific stage or chemically or biologically induced) (for example transit or signal peptides, time- or location-specific promoters). Such sequences are known to the person skilled in the art (see, for example, Braun et al., 1992, EMBO J. 11: 3219-3227; Wolter et al., 1988, Proc. Natl. Acad. Sei. USA 85: 846-850; Sonnewald et al ., 1991, Plant J. 1: 95-106).

The transgenic plant cells can be regenerated into whole plants using known techniques.

In this way, transgenic plants can be obtained which have altered properties as a result of overexpression, suppression or inhibition of homologous (i.e. endogenous) genes or gene sequences or expression of heterologous (i.e. exogenous) genes or gene sequences.

The method according to the invention is suitable for controlling a large number of harmful organisms which occur in particular in vegetables, in particular insects and arachnids, very particularly preferably insects. The pests mentioned include:

From the order of the Isopoda, for example, Armadillidium spp., Oniscus spp.,

Porcellio spp ..

From the order of the Diplopoda, for example, Blaniulus spp ..

From the order of the Chilopoda, for example Geophilus spp., Scutigera spp ..

From the order of the Symphyla, for example, Scutigerella spp ..

From the order of the Thysanura, for example, Lepisma spp ..

From the order of the Collembola, for example Onychiurus spp ..

From the order of the Orthoptera, for example Blattella spp., Periplaneta spp.,

Leucophaea spp., Acheta spp., Gryllotalpa spp., Locusta migratoria migratorioides,

Melanoplus spp., Schistocerca spp .. From the order of the Dermaptera, for example Forficula spp ..

From the order of the Isoptera, for example Reticulitermes spp., Reticulitermes spp.,

Coptotermes spp ..

From the order of the Thysanoptera, for example Frankliniella spp., Kakothrips spp.,

Hercinothrips spp., Scirtothrips spp., Taeniothrips spp., Thrips spp ..

From the order of the Heteroptera, for example Eurygaster spp., Stephanitis spp.,

Lygus spp., Aelia spp., Eurydema spp., Dysdercus spp., Piesma spp ..

From the order of the Homoptera, for example Aleurodes spp., Bemisia spp.,

Trialeurodes spp., Brevicoryne spp., Cryptomyzus spp., Aphis spp., Eriosoma spp.,

Hyalopterus spp., Phylloxera spp., Pemphigus spp., Macrosiphum spp., Myzus spp.,

Phorodon spp., Rhopalosiphum spp., Empoasca spp., Euscelis spp., Eulecanium spp., Saissetia spp., Aonidiella spp., Aspidiotus spp., Nephotettix spp., Laodelphax spp., Nilaparvataocpp., Sogatella .-, Psylla spp., Aphrophora spp., Aeneolamia spp.

From the order of the Lepidoptera, for example, Pectinophora spp., Bupalus spp.,

Cheimatobia spp., Cnephasia spp., Hydraecia spp., Lithocolletis spp., Hyponomeuta spp., Plutella spp., Plutella xylostella, Malacosoma spp., Euproctis spp., Lymantria spp., Bucculatrix spp., Phytomipalpa. , Phthorimaea spp.,

Gnorimoschema spp., Autographa spp., Evergestis spp., Lacanobia spp., Cydia spp., Pseudociaphila spp., Phyllocnistis spp., Agrotis spp., Euxoa spp., Feltia spp.,

Earias spp., Heliothis spp., Helicoverpa spp., Bombyx spp., Laphygma spp.,

Mamestra spp., Panolis spp., Prodenia spp., Spodoptera spp., Trichoplusia spp.,

Carpocapsa spp., Pieris spp., Chilo spp., Ostrinia spp., Pyrausta spp., Ephestia spp., Galleria spp., Cacoecia spp., Capua spp., Choristoneura spp., Clysia spp.,

Hofmannophila spp., Homona spp., Tineola spp., Tinea spp., Tortrix spp ..

From the order of the Coleoptera, for example, Anobium spp., Rhizopertha spp.,

Bruchidius spp., Acanthoscelides spp., Hylotrupes spp., Aclypea spp., Agelastica spp., Leptinotarsa spp., Psylliodes spp., Chaetocnema spp., Cassida spp.,

Bothynoderes spp., Clivina spp., Ceutorhynchus spp., Phyllotreta spp., Apion spp.,

Sitona spp., Bruchus spp., Phaedon spp., Diabrotica spp., Psylloides spp.,

Epilachna spp., Atomaria spp., Oryzaephilus spp., Anthonomus spp., Sitophilus spp., Otiorhynchus spp., Cosmopolites spp., Ceuthorrynchus spp., Hypera spp.,

Dermestes spp., Trogoderma spp., Anthrenus spp., Attagenus spp., Lyctus spp.,

Meligethes spp., Ptinus spp., Niptus spp., Gibbfum spp., Tribolium spp., Tenebrio spp., Agriotes spp., Conoderus spp., Melolontha spp., Amphimallon spp., Costelytra spp ..

From the order of the Hymenoptera, for example Diprion spp., Hoplocampa spp.,

Lasius spp., Monomorium spp., Vespa spp ..

From the order of the Diptera, for example Drosophila spp., Chrysomyxa spp.,

Hypoderma spp., Tannia spp., Bibio spp., Oscinella spp., Phorbia spp., Pegomyia spp., Ceratitis spp., Dacus spp., Tipula spp ..

From the Arachnida class, for example, Scorpio maurus, Latrodectus mactans.

From the order of the Acarina, for example Acarus spp., Bryobia spp., Panonychus spp., Tetranychus spp., Eotetranychus spp., Oligonychus spp., Eutetranychus spp.,

Eriophyes spp., Phyllocoptruta spp., Tarsonemus spp., Rhipicephalus spp.,

Rhipicephalus spp., Ixodes spp., Amblyomma spp ..

From the class of the Helminths, for example Meloidogyne spp., Heterodera spp.,

Globodera spp., Radopholus spp., Pratylenchus spp., Tylenchulus spp.,

Tylenchorhynchus spp., Rotylenchus spp., Heliocotylenchus spp., Belonoaimus spp., Longidorus spp., Trichodorus spp., Xiphinema spp., Ditylenchus spp.,

Aphelenchoides spp., Anguina spp ..

From the class of the Gastropoda, for example, Deroceras spp., Arion spp., Lymnaea spp., Galba spp., Succinea spp ..

The method according to the invention is preferably suitable for controlling Agriotes spp., Melolontha spp., Aphis spp., Cnephasia spp., Ostrinia spp., Agrotis spp., Hydraecia spp., Tipula spp., Myzus spp., Bemisia spp., Trialeurodes spp., Oscinella spp., Tetranychus spp., Lygus spp., Leptinotarsa spp., Psylliodes spp., Phytometra spp., Deroceras spp., Psylla spp., Blaniulus spp., Onychiurus spp., Piesma spp., Atomaria spp. , Aclypea spp., Chaetocnema spp., Cassida spp., Bothynoderes spp., Clivina spp., Scrobipalpa spp., Phthorimaea spp., Gnorimoschema spp., Mamestra spp., Autographa spp., Arion spp., Gryllotalpa spp., Eurydema spp., Meligethes spp., Ceutorhynchus spp., Phyllotreta spp., Plutella xylostella, Evergestis spp., Lacanobia spp., Pieris spp., Forficula spp., Hypera spp., Apion spp., Otiorhynchus spp., Sitona spp. , Acanthoscelides spp., Kakothrips spp., Bruchus spp., Cydia spp., Thrips spp., Pseudociaphila spp., Heliothis spp., Helicoverpa spp., Prodenia spp., Spodoptera spp., Chilo spp. And Diabrotica spp ..

The invention is illustrated by the following examples without restricting it.

example

Plutella xylostella (vegetable moth, cabbage cockroach)

Seven-week-old cauliflower plants (ordinary cauliflower, Marner Allfrüh variety) and Bt cauliflower (Bt resistant strain, contains cry9C) were sprayed with the insecticides to be tested using a track sprayer (200 l / ha). After drying, plants were infected with 5 L2 larvae and 5 L3 larvae of Plutella xylostella. After 2, 4 and 7 days (DAA), feeding damage and mortality were rated. The test was carried out in a greenhouse at 23 ° C and 60% rel. Moisture carried out.

% Feeding damage /% mortality

Connection 2 DAA 4 DAA 7 DAA

BlumenBt .- BlumenBt.- BlumenB1 g active ingredient / ha kohl cauliflower cauliflower cauliflower kohl kohl

Control - 10 20 10 0 15 20 15 20 15 20 15 30

Tolfenpyrad 100 5 0 10 20 5 20 10 100 5 0 10 100

EC15 30 10 0 0 60 10 0 0 100 10 0 0 100

10 10 0 10 0 10 0 10 50 10 0 10 50

3 10 0 3 10 10 0 10 30 10 0 10 50

Fipronil 100 1 100 0 100 1 100 0 100 1 100 0 100

SC05 30 4 70 0 100 5 100 0 100 5 100 0 100

10 8 20 0 100 8 20 0 100 10 50 0 100

3 10 20 0 100 10 20 0 100 12 20 0 100

1 10 0 0 70 10 0 0 100 12 0 0 100

Thiodicarb 100 10 0 0 100 10 0 0 100 10 0 0 100

SC33 30 10 0 0 100 10 0 0 100 15 0 0 100

10 10 0 0 90 10 0 0 100 15 0 0 100

3 10 0 0 100 10 0 0 100 15 0 0 100

1 10 0 0 100 10 0 0 100 15 0 0 100

Acetamiprid 30 10 0 0 100 12 0 0 100 15 0 0 100

WP20 10 10 0 0 100 12 0 0 100 15 0 0 100

A significant synergistic effect is clearly recognizable with all four active ingredients.

Claims

claims:

1. A method for controlling harmful organisms in genetically modified vegetables which contain a gene derived from Bacillus thuringiensis, which codes for and expresses an insecticidally active protein, characterized in that an insecticidally effective amount of one or more compounds from the following groups (a) to (f) on the plants, their seeds or propagation material and / or their cultivated area:

a) Insecticidal organophosphorus compounds from the group: Acephate, Azinphos-ethyl, Azinphos-methyl, Cadusafos, Chlorfenvinphos, Chlormephos, Chlorpyrifos, Demeton-S-methyl, Diazinon, Dicrotophos, Dimethoate, Disulfoton, Ethion, Ethoprophos, Eofimazos Isofenphos, Malathion, Methamidophos, Methidathion, Mevinphos, Monocrotophos, Omethoate, Parathion, Phenthoate, Phorate, Phosalone, Phosmet, Phosphamidon, Phoxim, Pirimiphos-methyl, Profenofos, Prothiofos, Pyridaphenthion, Quinalphos.Tlorufos; Trerbufos; Trerbinos

b) pyrethroids from the group:

Acrinathrin, Allethrin, Bifenthrin, Cycloprothrin, Cyfluthrin, (Beta) -Cyfluthrin, (Lambda) -Cyhalothrin, Cypermethrin, (Alpha) -Cypermethrin, (Beta) -Cypermethrin, (Zeta) -Cypermethrin, Deltamerhrate, Fenfenvalhrin, Fenfenvalhrin, Esfenpathrin, Esfenpathrin Flucythrinate, (tau) fluvalinate, permethrin, tefluthrin, tralomethrin and ZXI 8901;

c) insecticidal carbamates from the group:

Alanycarb, Aldicarb, Amitraz, Bendiocarb, Benfuracarb, Butocarboxim, Carbaryl, Carbofuran, Carbosulfan, Ethiofencarb, Formetanate, Furathiocarb, Isoprocarb, Methiocarb, Methomyl, Oxamyl, Pirimicarb, Propoxur, Thiofanox, Thacarbarbox; d) Biopesticides from the group:

Bacillus thuringiensis, Bacillus firmus, granular and nuclear polyhedron viruses, Beauveria bassiana, Beauveria brogniartii and Baculoviruses such as Autographa California;

e) insecticidal growth regulators from the group: chlorfluazuron, DBI-3204, diflubenzuron, flucycloxuron, flufenoxuron, hexaflumuron, lufenuron, novaluron, methoxyfenozide, teflubenzuron, tebufenozide and triflumuron;

f) Other:

Abamectin, Bensultap, Cartap, Chlordane, Chlorfenapyr, DNOC, Endosulfan, Fipronil, Ethiprole, Imidacloprid, Thiacloprid, Phosphine, Oleic Acid / Fatty Acids, Pymetrozine, Thiocyclam, IKI-220, Tolfenpyrad, Acetamethoxafluid, Spinosad.

2. The method according to claim 1, characterized in that a compound from the group of insecticidal organophosphorus compounds (a), pyrethroids (b), insecticidal carbamates (c), insecticidal growth regulators (e), abamectin, acetamiprid, chlorfenapyr, endosulfan, fipronil, Ethiprole, Imidacloprid, Pymetrozine, Spinosad, Silafluofen, Thiamethoxam, Thiacloprid, Tolfenpyrad, IKI-220 and Bacillus thuringiensis are used.

3. The method according to claim 2, characterized in that a compound from the group Ethoprophos, Malathion, Parathion, Phosalone, Pirimiphos-methyl, Triazophos, Acrinathrin, Deltamethrin, Tefluthrin, Aldicarb, Amitraz, Bendiocarb, Carbaryl, Carbofuran, Oxamyl, Pirimicarb , Thiodicarb, Diflubenzuron, Lufenuron, Novaluron, Methoxyfenozide, Tebufenozide, Abamectin, Acetamiprid, Chlorfenapyr, Endosulfan, Fipronil, Ethiprole, Imidacloprid, Pymetrozine, Spinosad, Silafluofen, Thiamethadridus, Thiaethyradrid, I used.

4. The method according to one or more of claims 1 to 3, characterized in that one uses a mixture of two or more of the insecticidally active compounds.

5. The method according to one or more of claims 1 to 4, characterized in that the insecticidally active compound is applied in an application rate of 0.0001 to 5.0 kg / ha.

6. The method according to one or more of claims 1 to 5, characterized in that insecticidally active compound is used as a 0.00001 to 95 wt .-% formulation.

7. The method according to one or more of claims 1 to 6, characterized in that the insecticidally active protein in the vegetable plant is a crystal protein from the subfamily cryl, cry2, cry3, cry5 and / or cry9.

8. The method according to claim 7, characterized in that the insecticidally active protein in the vegetable plant is crylAb, crylAc, cry3A, cry3B and / or cry9C.

9. The method according to one or more of claims 1 to 8, characterized in that vegetable plants are used which have a glufosinate or glyphosate resistance.

10. The method according to one or more of claims 1 to 9, characterized in that the harmful organisms are insects which belong to the orders Homoptera, Lepidoptera and / or Coleoptera.

11. The method according to one or more of claims 1 to 10, characterized in that the insecticidally active compound against eggs and / or against larvae in the L1 and / or L2 and / or L3 and / or L4 stage and / or L5 Stage and / or against adult animals of the harmful organisms.

12. The method according to one or more of claims 1 to 11, characterized in that one or more further insecticidally active, fungicidal or herbicidally active compounds are used in addition to one or more insecticidally active compounds from group (a) to (f).

14. Use of compounds from the following groups (a) to (f)

a) Insecticidal organophosphorus compounds from the group: Acephate, Azinphos-ethyl, Azinphos-methyl, Cadusafos, Chlorfenvinphos, Chlormephos, Chlorpyrifos, Demeton-S-methyl, Diazinon, Dicrotophos, Dimethoate, Disulfoton, Ethion, Ethoprophos, Eofimazos Isofenphos, Malathion, Methamidophos, Methidathion, Mevinphos, Monocrotophos, Omethoate, Parathion, Phenthoate, Phorate, Phosalone, Phosmet, Phosphamidon, Phoxim, Pirimiphos-methyl, Profenofos, Prothiofos, Pyridaphenthion, Quinalphos.Tlorufos; Trerbufos; Trerbinos

b) pyrethroids from the group:

Acrinathrin, Allethrin, Bifenthrin, Cycloprothrin, Cyfluthrin, (Beta) -Cyfluthrin, (Lambda) -Cyhalothrin, Cypermethrin, (Alpha) -Cypermethrin, (Beta) -Cypermethrin, (Zeta) -Cypermethrin, Deltamerhrate, Fenfenvalhrin, Fenfenvalhrin, Esfenpathrin, Esfenpathrin Flucythrinate, (tau) fluvalinate, permethrin, tefluthrin, tralomethrin and ZXI 8901;

c) insecticidal carbamates from the group:

Alanycarb, Aldicarb, Amitraz, Bendiocarb, Benfuracarb, Butocarboxim, Carbaryl, Carbofuran, Carbosulfan, Ethiofencarb, Formetanate, Furathiocarb, Isoprocarb, Methiocarb, Methomyl, Oxamyl, Pirimicarb, Propoxur, Thiofanox, Thacarbarbox;

d) Biopesticides from the group: Bacillus thuringiensis, Bacillus firmus, granular and nuclear polyhedron viruses, Beauveria bassiana, Beauveria brogniartii and Baculoviruses such as Autographa California;

e) insecticidal growth regulators from the group: chlorfluazuron, DBI-3204, diflubenzuron, flucycloxuron, flufenoxuron, hexaflumuron, lufenuron, novaluron, methoxyfenozide, teflubenzuron, tebufenozide and triflumuron;

f) Other:

Abamectin, Bensultap, Cartap, Chlordane, Chlorfenapyr, DNOC, Endosulfan, Fipronil, Ethiprole, Imidacloprid, Thiacloprid, Phosphine, Oleic Acid / Fatty Acids, Pymetrozine, Thiocyclam, IKI-220, Tolfenpyrad, Acetamethoxafluid, Spinosad;

for controlling harmful organisms in genetically modified vegetable plants which contain a gene derived from Bacillus thuringiensis, which encodes and expresses an insecticidally active protein.