EP1349929A2 - Apoptosis-inducing dna sequences - Google Patents

Apoptosis-inducing dna sequences

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Publication number
EP1349929A2
EP1349929A2 EP01954041A EP01954041A EP1349929A2 EP 1349929 A2 EP1349929 A2 EP 1349929A2 EP 01954041 A EP01954041 A EP 01954041A EP 01954041 A EP01954041 A EP 01954041A EP 1349929 A2 EP1349929 A2 EP 1349929A2
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European Patent Office
Prior art keywords
clone
gene
nucleic acid
identity
mrna
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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EP01954041A
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German (de)
French (fr)
Inventor
Stefan Grimm
Nicole SCHÖNFELD
Erik Braziulis
Ursula Cramer
Andreas Gewies
Frank Voss
Thomas Mund
Timur Albayrak
Hendrik Gille
Matthias Klein
Manuel Bauer
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Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
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Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
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Priority claimed from DE10126344A external-priority patent/DE10126344A1/en
Application filed by Max Planck Gesellschaft zur Foerderung der Wissenschaften eV filed Critical Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Publication of EP1349929A2 publication Critical patent/EP1349929A2/en
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4747—Apoptosis related proteins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy

Definitions

  • the invention relates to new apoptosis-associated and in particular apoptosis-inducing nucleic acid sequences, polypeptides encoded thereof and their use for the provision of diagnostic and therapeutic agents.
  • the invention relates to transgenic cell systems and animals and their use for the genetic and / or pharmacological investigation of apoptosis-associated diseases.
  • Apoptosis is the genetically encoded suicide program that is induced in eukaryotic cells under certain physiological or pathological conditions.
  • the induction of apoptosis must be regulated extremely precisely because hyperactivity can lead to degenerative diseases.
  • reduced apoptosis induction can contribute to tumor progression.
  • plasmid pools of 20 clones are used for transfection into the kidney cell line.
  • the transfection of plasmid pools can only achieve a low sensitivity for the screening method, since those expression plasmids which contain apoptosis-inducing genes are present in each case in a low ratio to the expression plasmids which do not carry apoptosis-inducing genes.
  • aliquots which contained only one clone statistically were used instead of plasmid pools in the screening method used for transfection.
  • a particularly effective way of purifying plasmid DNA was used (Neudecker and Grimm, Biotechniques 28 (2000), 107-109).
  • This procedure enables Reduction of lipopolysaccharides by a factor of 900 compared to previously published methods and thus enables for the first time the purification of plasmid DNA for high throughput in the "96 well" format.
  • the use of specially purified DNA for transfection in combination with the use of individual clones instead of plasmid pools enabled a particularly effective and sensitive screening process to be carried out, which made it possible to determine a large number of apoptosis-inducing sequences.
  • the present invention relates to new apoptosis-associated and in particular apoptosis-inducing nucleic acids comprising: (a) the nucleic acids of clones 1 - 1 6 shown in Table 1, in Table 2 or in SEQ ID NO: 1 - 225, and nucleic acids complementary thereto or fragments thereof, (b) nucleic acids corresponding to the sequences according to (a) in the context of the degeneration of the genetic code and (c) nucleic acids hybridizing with the sequences according to (a) or / and (b) under stringent conditions.
  • the nucleic acids according to the invention are apoptosis-associated nucleic acids, ie nucleic acids which are associated with the occurrence of apoptotic processes in a cell, in particular in a mammalian cell.
  • the nucleic acids are preferably apoptosis-inducing nucleic acids, ie nucleic acids which can induce and / or promote apoptotic processes.
  • the nucleic acids according to the invention are particularly preferably dominant apoptosis-inducing nucleic acids which are capable of inducing apoptosis when expressed in a cell and which produce the characteristics characteristic of apoptosis, such as DNA fragmentation, morphological peculiarities etc.
  • the nucleic acids can be in double-stranded or single-stranded form, for example as DNA or RNA.
  • the isolated nucleic acids can develop their cellular effect by expression, in particular by overexpression in cells. This makes them inducible and defines their use as a therapeutic agent.
  • nucleic acids or partial fragments thereof shown in Table 1 or the corresponding sequence protocols with a length of preferably at least 15, particularly preferably at least 20 and most preferably at least 25 nucleotides, variants of these sequences are also covered by the present invention.
  • nucleic acids which correspond to the sequences according to (a) in the context of the degeneration of the genetic code and which code for a polypeptide with the same amino acid sequence nucleic acids are also recorded which are stringent with the sequences according to (a) or / and (b) Hybridize conditions.
  • hybridization under stringent conditions means that after pre-hybridization and hybridization under suitable conditions and washing in 1 ⁇ SSC and 0.1% SDS at 55 ° C., preferably at 62 ° C. and particularly preferably at 68 ° C. and in particular a hybridization signal is also found in 0.2 x SSC and 0.1% SDS at 55 ° C, preferably at 62 ° C and particularly preferably at 68 ° C (see also Sambrook et al., Molecular Cloning. A Laboratory Manual (1 989), Cold Spring Harbor Laboratory Press, 1 .101 -1 .104).
  • the apoptosis-associated nucleic acids according to the invention preferably code for an apoptosis-associated polypeptide or a functional fragment thereof.
  • the nucleic acids can originate from any organism, eukaryotic organisms such as nematodes, for example C. elegans, arthropods such as Drosophila, Cordata and vertebrates, for example mammals, being preferred. Sequences from mammals, for example from the mouse or from humans, are particularly preferred, these sequences optionally being able to be modified by known molecular biological techniques, such as site-specific mutagenesis, PCR, restriction cleavage and ligation.
  • the nucleic acids according to the invention are preferably operatively linked to an expression control sequence so that they can be transcribed and, if necessary, translated in a suitable host cell.
  • Expression control sequences usually comprise a promoter and optionally regulatory sequences such as operators or enhancers. Translation initiation sequences may also be present.
  • Suitable expression control sequences for prokaryotic or eukaryotic host cells are known to the person skilled in the art (see, for example, Sambrook et al., Supra).
  • Another object of the invention is a recombinant vector which contains a nucleic acid according to the invention, preferably in operative linkage with an expression control sequence.
  • the recombinant vector can also contain common elements such as an origin of replication and a selection marker gene.
  • suitable recombinant vectors e.g. Plasmids, cosmids, phages, viruses etc. are known to the person skilled in the art (see e.g. Sambrook et al., Supra).
  • the invention further relates to recombinant cells which can be transformed or transfected with a nucleic acid or a vector according to the invention.
  • the transformation or transfection can be carried out according to known methods, e.g. by calcium phosphate coprecipitation, lipofection, electroporation, particle bombardment or viral infection.
  • the cell according to the invention can contain the recombinant nucleic acid in extrachromosomal or chromosomally integrated form.
  • Another object of the invention is apoptosis-associated polypeptides which are encoded by a nucleic acid according to the invention.
  • Apoptosis-associated polypeptides can be obtained by expression of the apoptosis-associated nucleic acids according to the invention, by chemical synthesis or by combinations of both methods.
  • Another object of the invention is a pharmaceutical composition which contains a nucleic acid according to the invention, a vector according to the invention or a polypeptide according to the invention optionally together with pharmaceutically customary excipients and auxiliaries.
  • the nucleic acids, vectors, cells and polypeptides described above can be used to produce a diagnostic or therapeutic agent, in particular an agent for the diagnosis, therapy or prevention of apoptosis-associated diseases.
  • Apoptosis-associated diseases can be characterized on the one hand by an abnormally reduced apoptosis and thus by hyperproiiferation, for example tumor diseases, autoimmune diseases and viral infections (Thompson, Science 267 (1995), 1456-1462).
  • apoptosis-associated diseases can also be characterized by abnormally increased apoptosis and thus by degenerative symptoms, such as Alzheimer's disease, Huntington's disease, Parkinson's disease, reperfusion damage, stroke and alcohol damage to the liver (Thompson (1995), supra ).
  • the diagnostic application comprises a qualitative and / or quantitative detection of the apoptosis-associated nucleic acid, for example in the form of a transcript, or the polypeptide encoded thereby in a sample, in particular a sample, which was taken from a diseased organism, for example a patient.
  • the detection can be carried out in the usual way, for example by nucleic acid hybridization or amplification reactions such as PCR or by protein detection using antibodies. Numerous techniques for this are known to the person skilled in the art.
  • the detection can also be done by using the isolated genes on a DNA chip. This allows several, eg all genes to be examined simultaneously in one experiment.
  • the therapeutic or preventive application comprises the administration of an active substance to a diseased organism in a sufficient dosage to alleviate or cure the apoptosis-associated disease or to prevent the onset of an apoptosis-associated disease.
  • an apoptosis-associated nucleic acid is administered on a gene therapy vector, for example an adenovirus, a retrovirus, an adeno-associated virus, etc., in order to bring about increased expression of the apoptosis-associated nucleic acid in a diseased target cell.
  • a gene therapy vector for example an adenovirus, a retrovirus, an adeno-associated virus, etc.
  • an antisense nucleic acid can also be administered, for example on a gene therapy vector or also directly, provided that the expression of the apoptosis-associated nucleic acid is to be reduced.
  • apoptosis-associated polypeptides or modulators of the activity of such apoptosis-associated polypeptides can be administered.
  • the active substances are administered by known methods, for example in gene therapy (Anderson, Nature 392 (1998), 25-30) or protein therapy (Schwarze et al., Science 285 (1999), 1569-1572).
  • nucleic acids, vectors, cells and polypeptides according to the invention can also be used to identify new active substances for the therapy or prevention of apoptosis-associated diseases.
  • the use in known cellular or molecular screening assays is conceivable here, in a high throughput format.
  • the invention naturally also relates to the active substances identified by the use of such screening methods or substances derived therefrom.
  • the active substances identified by the screen are able to activate or inhibit signaling pathways which are induced by the expression of the nucleic acids.
  • the present invention further provides transgenic non-human animals which (i) constitutively or inducibly overexpress the gene of a nucleic acid according to the invention or the ANT-1 gene, (ii) the endogenous gene of a nucleic acid according to the invention or the ANT-1 gene in inactivated form Contain form, (iii) contain the endogenous gene of a nucleic acid according to the invention or the ANT-1 gene completely or partially replaced by a mutated gene of a nucleic acid according to the invention or a mutated ANT-1 gene, (iv) conditional and tissue-specific overexpression or underexpression of the gene of a nucleic acid according to the invention or of the ANT-1 gene or (v) have a conditional and tissue-specific knock-out of the gene of a nucleic acid according to the invention or of the ANT-1 gene.
  • the transgenic animal can preferably additionally contain an exogenous gene of a nucleic acid according to the invention or an exogenous ANT-1 gene under the control of a promoter which allows overexpression.
  • the endogenous gene of a nucleic acid according to the invention or the endogenous ANT-1 gene can be overexpressed by activation or / and exchange of the own promoter.
  • the endogenous promoter of the gene of a nucleic acid according to the invention or of the ANT-1 gene preferably has a genetic change which leads to a changed expression of the gene.
  • the genetic modification of the endogenous promoter includes a mutation of individual bases as well as deletion and insertion mutations.
  • a first embodiment relates to a transgenic animal which constitutively or inducibly overexpresses the gene of a nucleic acid according to the invention or the ANT-1 gene.
  • the introduced gene of a nucleic acid according to the invention or the introduced ANT-1 gene can optionally have additional mutations.
  • a second embodiment relates to a transgenic animal which contains the endogenous gene of a nucleic acid according to the invention or the endogenous ANT-1 gene in inactivated form.
  • the inactivation of the gene of a nucleic acid of the invention or of the ANT-1 gene is preferably effected by introducing a knock-out mutation "by homologous recombination or by introducing an antisense construct or an RNAi Kontrukts.
  • ANT-1 gene is completely or partially replaced by a mutated gene of a nucleic acid of the invention or a mutated ANT-1 gene.
  • a fourth embodiment relates to a transgenic animal which has a conditional and tissue-specific overexpression or underexpression of the gene of a nucleic acid according to the invention or of the ANT-1 gene.
  • the transgenic animal has a conditional and tissue-specific knock-out of the gene of a nucleic acid according to the invention or of the ANT-1 gene.
  • the transgenic animal is preferably a mammal, such as a rodent, for example a mouse.
  • Mice have numerous advantages over other animals. They are easy to hold and their physiology is considered a model system for that of humans.
  • the production of such gene-manipulated animals is well known to the person skilled in the art and is carried out by customary methods (Hogan, B., Beddington, R., Costantini, F. and Lacy, E. (1994), Manipulating the Mouse-Embryo; A Laboratory Manual, 2nd ed., Can Spring Harbor Laboratory, Cold Spring Harbor, NY).
  • the invention further relates to the use of such a transgenic animal for the genetic and / or pharmacological examination of diseases which are associated with excessive or reduced or no expression of a gene of a nucleic acid according to the invention or of an ANT-1 gene.
  • the transgenic animals according to the invention can serve as a model for the diseases associated with the gene of a nucleic acid or ANT-1 gene according to the invention in humans or also in farm animals. For example, the effects of drugs or gene therapies on the course of the disease can be determined. The animals can also be useful for diagnosis or early detection of a disease.
  • a transgenic animal according to the invention which contains the ANT-1 gene can serve as a model for the degenerative heart disease dilatoric cardiomyopathy (DCM).
  • DCM degenerative heart disease
  • This degenerative heart disease is associated with excessive apoptosis in a patient's heart cells.
  • a first indication that the apoptosis inducer ANT-1 plays an important role in the induction of apoptosis in DCM was the observation that the expression pattern of the ANT-1 isoforms in the patient developed very early in the course of DCM Heart shifts. There is an increased expression of ANT-1 mRNA and ANT-1 protein (PCT / EP00 / 0881 2).
  • the ANT-1 gene can be expressed in transgenic mice under the control of the heart-specific ⁇ r-myosin heavy chain promoter (Subramaniam, A. (1 991), J. Biol. Chem. 266 (36), pages 24613-24620) become. This promoter is well characterized and is only activated at the time of birth.
  • the expression construct can be produced, for example, by inserting the ANT-1 gene into the Sall restriction site of the third non-coding exon of the 5.5 kB promoter.
  • the hearts of the transgenic animals according to the invention thus produced should contain some of the cellular ones which are specific to DCM Changes such as fibrinization, apoptosis and hypertrophy, or functional disorders such as left ventricular pressure, end diastolic pressure, contractility, left ventricular ejection fraction and left ventricular filling pressure.
  • cell culture systems in particular human cell culture systems, can also be used for the applications which are described for the transgenic animal.
  • the invention is further illustrated by the following example.
  • sequence listing contains the sequences SEQ ID No. 1-225, which include the T7 sequences, BGH sequences and internal primer sequences of the identified apoptosis-inducing genes of clones 1-124 listed in Table 1.
  • Table 2 shows the sequences of clones 1-124 and 125, 127, 133, 134, 140, 141, 144, 145 and 146.
  • Apoptosis-inducing genes were found by a genetic screen in the human cell line HEK 293T (Grimm and Leder (1997), supra), which is based on the iterative transfection of small expression plasmid pools from a normalized gene library and the subsequent microscopic determination of the programmed cell death the phenotype of the apoptotic cells.
  • the transfection of individual clones from a positive plasmid pool then allows the apoptosis-inducing gene to be determined.
  • This screen was done in a 96-well format. Furthermore, a particularly effective way of purifying the plasmid DNA was used (Neudecker and Grimm, Biotechniques 28 (2000), 107-109).
  • Human HEK 293T cells were supplemented in DMEM with 5% fetal calf serum (Sigma, Deisenhofen, Germany) in a humidified 5% CO 2 atmosphere. For transfections, the cells were placed in 24-well plates and treated with 2 ⁇ g plasmid DNA using the calcium phosphate coprecipitation method as described by Roussel et al. (Mol. Cell. Biol. 4 (1984), 1999-2009).
  • Kidney mRNA from 10 week old CD1 mice was normalized by association of abundant mRNA species with antisense cDNA molecules covalently coupled to latex beads and subsequent separation by centrifugation. After two rounds of hybridization, 200 ng (from originally 2 ⁇ g) of mRNA were obtained and used to prepare a cDNA library using a cDNA synthesis kit (Gibco BRL, Gaithersburg, MD) used.
  • a cDNA synthesis kit Gibco BRL, Gaithersburg, MD
  • the cDNA molecules were inserted into a modified pcDNA3 vector (Invitrogen) under the control of the cytomegalovirus (CMV) promoter in which the neomycin resistance gene had been deleted.
  • CMV cytomegalovirus
  • the DNA was introduced by electroporation into E. coli SURE cells (Stratagene, Corp. La Jolla, CA), which were then immediately frozen.
  • the apoptosis-inducing activity of the transfected nucleic acids was carried out by microscopic determination of the cell phenotype. In the case of apoptotic cells, the optical density of the cells increases because the cytoplasmic nucleus volume ratio decreases and as a result of the breakdown of the cytoskeleton, bubbles form in the cytoplasmic membrane.
  • 96-hole blocks with bacteria were centrifuged for 5 min at 3000 g (Sigma centrifuges, Osterode am Harz, Germany). The supernatant was decanted and the blocks were inverted for 2-3 minutes. Then 1 70 ⁇ ⁇ buffer P1 (50 mM Tris-HCl / 1 OmM EDTA pH 8.0) was added and the bacterial pellets were resuspended by complete vortex treatment for 10 to 20 min. After adding 1 70 ⁇ ⁇ buffer P2 (200 mM NaOH, 1% SDS), the block was sealed with film, mixed by inverting and incubated for 5 min at room temperature. The lysis was terminated by adding 170 ⁇ l of 4 ° C.
  • the supernatant after centrifugation for 10 min at 6000 rpm was placed in 96-hole polyoxymethylene microtiter blocks. 1 50 ul silica suspension was added and incubated for 20 min at room temperature. The plates were centrifuged for 5 minutes at 6000 rpm. The supernatant was carefully decanted and 400 ul acetone (-20 ° C) was added. The plates were again vortexed (30 sec) and centrifuged for 3 min at 6000 rpm. This acetone washing process was repeated once. The plates were first dried at room temperature for 5 minutes and then for 5 minutes in a vacuum chamber.
  • the pellets were resuspended in 75 ul water (60 ° C) and centrifuged at 6000 rpm and 4 ° C for 10 min. The supernatant was stored in a 96-well microtiter plate at -20 ° C.
  • Clone 1 is composed of the following sequences: T7, BGH sequence, internal primer on the left and internal primer on the right (Seq. 1-4). The overall sequence is given under Contig (Seq. 5). Clone 1 shows homology with the following ESTs (expressed sequence tags): uc81 e12.y1 Sugano mouse kidney mkia Mus musculus cDNA clone (Genbank Acc. No AA986577.1
  • Clone 2 is composed of the T7 and BGH sequences. The overall sequence is given under Contig. Clone 2 shows homology with the following database entries: Homo sapiens cDNA FLJ20625 fis, clone KAT04008 (DNA Data Bank of Japan (DDBJ) Acc. No AK000632.1 and AK000632) with an identity of 91%; mf71 g09.r1 Soares mouse embryo NbME1 3.5 14.5 Mus musculus cDNA clone (Genbank Acc. No W89554.1 J W89554) with an identity of 99%; G0103H 1 2-3 Mouse E7.5 Embryonic Portion cDNA Library Mus musculus cDNA clone (Genbank Acc. No AW536394.1 j AW536394) with an identity of 99%.
  • Clone 3 is composed of the T7 and BGH sequences. The overall sequence is given under Contig. Clone 3 shows homology with EMBL Acc. No X78936
  • Clone 4 is composed of the T7 and BGH sequences. The overall sequence is given under Contig. Clone 4 shows homology with Mus musculus mRNA for calcium Channel gamma 5 subunit (CACNG5 gene) (EMBL Acc. No AJ272046.1 J MMU272046) with an identity of 97%.
  • CACNG5 gene calcium Channel gamma 5 subunit
  • Clone 5 shows homology with RefSeq database Acc. No NM_00821 8.1: Muscle hemoglobin alpha, adult chain 1 (Hba-a1), mRNA with one Length of 564bp and an identity of 97% as well as Genbank Acc. No L75940.1 1 MUSALGL: Mus muscle alpha-globin mRNA, complete cds with an identity of 98%.
  • Clone 6 is composed of T7 or R2 and BGH or 4SP6 sequence. The overall sequence is given under Contig. Clone 6 shows homology with the following database entries: Mus musculus domesticus mitochondrial carrier homolog 1 isoform a mRNA, complete cds; nuclear gene for mitochondrial (Genbank Acc. No AF176007.2 J AF176007) with an identity of 99%; Homo sapiens CGI-64 protein mRNA, complete cds (Genbank Acc. No AF151822.1
  • Clone 7 shows homology to Genbank Acc. No AF151893.1
  • AF151893 Homo sapiens CGI-135 protein mRNA, complete cds with an identity of 86%.
  • Clone 8 shows homology to RefSeq Acc. No NM_012504.1: Rattus norvegicus ATPase, Na + K -t-transporting, alpha 1 polypeptide (Atp1 a1), mRNA with an identity of 94%.
  • Clone 9 shows homology to the following database entries: Mus musculus solute carrier family 27 (fatty acid transporter), member 2 (Slc27a2), mRNA (RefSeq Acc. No NM_01 1978.1) with an identity of 99%; Mus muscle fatty acid transport protein 2 mRNA, complete cds (Genbank Acc. No AF072757.1 J AF072757) with an identity of 99%; Mus muscle mRNA for very-long-chain acyl-CoA synthetase (VLACS) (EMBL Acc. No AJ223958.1 J MMAJ3958) with an identity of 99%.
  • VLACS very-long-chain acyl-CoA synthetase
  • Clone 10 is composed of the T7 and BGH sequences. The overall sequence is given under Contig. Clone 10 shows homology EMBL Acc. No AL080066.1 J HSM800567: Homo sapiens mRNA; cDNA DKFZp564J 142 (from clone DKFZp564J142) with an identity of 89-95% as well as to DDBJ Acc. No AK001993.1
  • AK001993 Homo sapiens cDNA FLJ1 1 131 fis, clone PLACE 1006325, highly similar to Homo sapiens mRNA; cDNA DKFZp564J142 with an identity of 89-95%.
  • Clone 1 1 shows homology to the following database entries: RefSeq Acc. No NM_009984.1: Mus muscle cathepsin L (Ctsl), mRNA with an identity of 96%; EMBL Acc. No X06086.1 1 MMMEPR: Mouse mRNA for major excreted protein (MEP) with an identity of 96%; Genbank Acc. No J02583.1 j MUSCPR: with an identity of 96%.
  • Clone 12 is composed of the T7 and BGH sequences. The overall sequence is given under Contig. Clone 12 shows homology to the following database entries: EMBL Acc. No AL137721.1
  • HSM802233 Homo sapiens mRNA
  • Genbank Acc. No L34839.1 j HUMTUM Homo sapiens over-expressed breast
  • Clone 13 is composed of the T7 and BGH sequences. The overall sequence is given under Contig. Clone 13 shows homology to Genbank Acc. No AF151807.1 JAF151807: Homo sapiens CGI-49 protein mRNA, complete cds with an identity of 87-90%. Clone 14 shows homology to Genbank Acc. No AF080469] AF080469: Mus muscle putative glycogen storage disease type 1b protein mRNA, complete cds with an identity of 94%.
  • Clone 15 shows an identity to Genbank Acc. No U31241
  • CGU31241 Cricetulus griseus integral membrane protein CII-3 mRNA, nuclear gene encoding mitochondrial protein, complete cds with an identity of 85% and to Genbank Acc. No S74803JS74803: CII-3 succinate-ubiquinone oxidoreductase complex II membrane-intrinsic subunit [cattle, heart, mRNA with an identity of 83%.
  • Clone 16 is composed of T7 or 4SP6 and BGH sequences. The overall sequence is given under Contig. Clone 16 shows homology to Genbank Acc. No AF116911.1
  • AF116911 Mus musculus thymic dendritic cell-derived factor 1 mRNA, complete cds with an identity of 96% and for RefSeq Acc. No NM_004872.1: Homo sapiens mouse tropomyosin homolog (HSPC001) mRNA with an identity of 89-92%.
  • Clone 17 shows an identity to Genbank Acc. No AF056031 JAF056031: Rattus norvegicus kynurenine 3-hydroxylase mRNA, complete cds with an identity of 90% and EMBL Acc. No Y1.3153JHSKYNU3MO: Homo sapiens mRNA for kynurenine 3-monooxygenase with an identity of 82%.
  • Clone 18 is composed of the T7 and BGH sequences. The overall sequence is given under Contig. Clone 18 shows homology to Genbank Acc. No AW109849.11 AW109849: MT2475 mouse liver, dioxin treated Mus musculus cDNA clone MT24753 'with an identity of 94%; Genbank Acc. No AA277327.1
  • Clone 19 shows homology to DDBJ Acc. No AB005451
  • AB005451 Mus musculus mRNA for RST, complete cds with an identity of 92%.
  • Clone 20 shows homology to Genbank Acc. No AA109018
  • Clone 21 shows homology to the following database entries: Genbank Acc. No M12673JRATGNPAS: RATGNPAS Rat guanine nucleotide-binding protein G-s, alpha subunit mRNA complete cds with an identity of 87% and EMBL Acc. No Y00703
  • MMGTPAMU Mouse uncoupled S49 cells mRNA for stimulatory GTP-binding protein alpha subunit with an identity of 87%; Genbank Acc. No M17525JRATBPGTPD: RATBPGTPD Rat GTP-binding protein (G-alpha-8) mRNA, complete cds with an identity of 87%; Genbank Acc. No AF116268
  • AF116268 Mus musculus G-protein XLAS (Xlas) mRNA, altematively spliced, complete cds with an identity of 94%.
  • Clone 22 shows homology to the following database entries: Genbank Acc. No AF061026
  • AF061026 Mus musculus leucine zipper-EF-hand containing transmembrane protein 1 (Letml) mRNA, complete cds with an identity of 93-95%.
  • Clone 23 shows homology to the following database entries: Genbank Acc. No AA086895JAA086895: mk19c02.r1 Soares mouse p3NMF19.5 Mus musculus cDNA clone 4933465 'similar to WP: F17C11.8 CE05655 and to Genbank Acc. No AA881548
  • Clone 24 shows homology to Genbank Acc. No U22465
  • MMU22465 Mus musculus Na / Pi-cotransporter (NaPi-7) mRNA, complete cds with an identity of 93% and to Genbank Acc. No L33878
  • MUSSPT Mus musculus renal sodium phosphate (Na + / Pi) transporter mRNA complete cds with an identity of 93%.
  • Clone 25 shows homology to Genbank Acc. No AF049882: Rattus norvegicus metastasis suppressor homolog (KAU) mRNA, complete cds and for DDBJ Acc.No D14883
  • MUSC33R2IA Mouse mRNA for C33 / R2 / IA4, complete cds with an identity of 94%.
  • C33-induced apoptosis is mediated by the induction of oxygen radicals, which lead to proapoptotic activation of mitochondria. These oxygen radicals are not generated by the mitochondrial respiratory chain, since cells with a genetic defect in the respiratory chain still respond to C33 expression with apoptosis.
  • the proapoptotic activity of C33 is not dependent on substrate or cell-cell interaction, since suspension cells also become apoptotic by C33.
  • the rapid kinetics of the apoptosis induction of C33 also differs from the previously claimed proapoptotic effects of C33.
  • an extracellular loop in C33 which apparently is responsible for the substrate interaction, is not necessary for the induction of apoptosis.
  • Clone 26 shows homology to Genbank Acc. No U50987
  • BTU50987 Bos taurus succinate-ubiquinone reductase membrane anchor subunit precursor QPs3 mRNA, complete cds with an identity of 86%
  • DDBJ AB006202 Homo sapiens mRNA for cytochrome b small subunit of complex II, complete cds with an identity of 82%.
  • Clone 27 shows homology to Genbank Acc. No M31775
  • MUSCYTB558 Mouse cytochrome beta-558 mRNA, 3 'end with an identity of 88%.
  • Clone 28 shows homology to Genbank Acc. No U76253 J MMU76253: Mus musculus E25B protein mRNA, complete cds with an identity of 88%.
  • Clone 29 shows homology to EMBL Acc. No Z98200.81 HS1 1 1 B22: Human DNA sequence from clone RP1-1 1 1 B22 on chromosome 6q16-21 Contains a novel pseudogene, a pseudogene similar to ribosomal protein L3, ESTs, STSs, GSSs and CpG Islands, complete sequence [ Homo sapiens] with an identity of 85% and Genbank Acc.
  • Clone 30 shows homology to the following database entries: Genbank Acc. No AI1 15883
  • AI31 5450 uj46g05.y1 Sugano mouse liver mlia Mus musculus cDNA clone IMAGE-.1 923032 5 ', mRNA sequence [Mus musculus] with an identity of 89%; Genbank Acc. No AI01 96781 AI01 9678: ua92d1 1 .r1 Soares mouse mammary gland NbMMG Mus musculus cDNA clone 1 364949 5 'with an identity of 89%;
  • Clone 32 shows homology to Genbank Acc. No U071 59
  • MMU071 59 Muscle medium-chain acyl-CoA dehydrogenase mRNA, complete cds with an identity of 94%.
  • Clone 33 shows homology to Genbank Acc. No M761 31 ⁇ MUSEF2: Mouse elongation factor 2 (ef-2) mRNA, 3 'end with an identity of 89)%.
  • Clone 34 shows homology to Genbank Acc. No AF072757 J AF072757: Mus musculus fatty acid transport protein 2 mRNA, complete cds with an identity of 96%.
  • Clone 35 shows homology to EMBL Acc. No X76453 J RNHREV1 07: R.norvegicus (Sprague Dawley) H-rev107 mRNA with an identity of 90% and EMBL Acc. No X92814J HSHREV107: H. sapiens mRNA for rat HREV107-Iike protein with an identity of 85%.
  • Clone 36 shows homology to Genbank Acc. No AW107362.1
  • Clone 37 shows homology to the following database entries: Genbank Acc. No AI31 5920
  • GIANT LARVAE HOMOLOGUE mRNA sequence [Mus musculus] with one Identity of 87%; Genbank Acc. No A1315072 J AI315072: uj ' 23e1 1.x1 Sugano mouse kidney mkia Mus musculus cDNA clone IMAGE: 1920812 3', mRNA sequence [Mus musculus] with an identity of 94%; Genbank Acc.
  • MMHC213L3 Mus musculus major histocompatibility locus class III regions Hsc70t gene, partial cds; smRNP, G7A, NG23, MutS homolog, CLCP, NG24, NG25, and NG26 genes, complete cds; and unknown genes with an identity of 82-96%; Genbank Acc.
  • MMHC425O18 Mus muscle major histocompatibility complex region NG27, NG28, RPS28, NADH oxidoreductase, NG29, KIFC1, Fas-binding protein, BING 1, tapasin, RalGDS-like, KE2, BING4, beta 1, 3-galactosyl transferase, and RPS18 genes with an identity of 82%.
  • Clone 39 shows homology to Genbank Acc. No AA763399 J AA763399: vw53h02.r1 Soares mouse mammary gland NMLMG Mus musculus cDNA clone 1247571 5 'similar to WP: F32D8.4 CE05783 LACTATE DEHYDROGENASE with an identity of 96%.
  • Clone 40 shows homology to Genbank Acc. No U00677
  • U00677 Mus musculus syntrophin-1 gene, complete cds with an identity of 92%.
  • Clone 41 shows homology to Genbank Acc. No AC003043 J AC003043: Homo sapiens chromosome 17, clone HRPC1067M6, complete sequence [Homo sapiens] with an identity of 87% and to Genbank Acc. No AA109006
  • Clone 42 shows homology to Genbank Acc. No U30838
  • MMU30838 Mus musculus voltage dependent anion Channel 2 mRNA, nuclear gene encoding mitochondrial protein, complete cds with an identity of 94% as well as Genabank Acc. No L08666 J HUMPORIN: Homo sapiens porin (por) mRNA, complete cds and truncated cds with an identity of 94%:
  • Clone 43 shows homology to the following database entries: Genbank Acc. No M27071 J MUSDIS2M1 A: Mus muscle protein phosphatase type 1 (dis2m1) mRNA, complete cds with an identity of 92%; Genbank Acc. No U53456
  • MMU53456 Muscle protein phosphatase 1 cgamma (PP1 cgamma) mRNA, complete cds with an identity of 98-100%; EMBL Acc. No X56438 J DMPP1A1: D.melanogaster PP1 -alpha 96A gene for protein phosphatase 1 with an identity of 80%; Genbank Acc.
  • Clone 44 shows homology to Genbank Acc. No U52842
  • MMU52842 Muscle kidney-specific transport protein mRNA, complete cds with an identity of 99% and on DDBJ Acc. No AB004559] AB004559: Rattus norvegicus mRNA for multispecific organic anion transporter, complete cds with an identity of 93%.
  • Clone 45 shows homology to the following database entries: Genbank Acc. No AF080252 J AF080252: Mus muscle serine / threonine protein kinase 51 PK (S) mRNA, complete cds with an identity of 94%; Genbank Acc. No AF080253
  • AF080253 Mus muscle serine / threonine protein kinase 51 PK (L) mRNA, complete cds with an identity of 94%; DDBJ Acc. No AB000449 J AB000449: Homo sapiens mRNA for VRK1, complete cds with an identity of 83%.
  • Clone 46 shows homology to the following database entries: Genbank Acc. No J04806 j MUSOSP: Muscle osteopontin mRNA, complete cds with an identity of 95%; EMBL Acc. No X1 3986
  • MMPONTIN Mouse mRNA for minopontin with an identity of 96%; EMBL Acc. No X 1 61 51
  • MMETA1 Mouse mRNA for early T-lymphocyte activation 1 protein (ETa-1) with an identity of 95%; Genbank Acc.
  • Clone 47 shows homology to DDBJ Acc. No AB006451
  • AB006451 Rattus norvegicus mRNA for Tim23, complete cds with an identity of 94% and Genbank Acc. No AF0301 62 J AF0301 62: Homo sapiens inner mitochondrial membrane translocase Tim23 (TIM23) mRNA, nuclear gene encoding mitochondrial protein complete cds with an identity of 89%.
  • Clone 48 shows homology to DDBJ Acc. No D38549 J HUMHA1025A: Human mRNA for KIAA0068 genes, partial cds with an identity of 86% and to Genbank Acc. No AF072697 J AF072697: Mus muscle SHYC (Shyc) mRNA, complete cds with an identity of 96%.
  • Clone 49 shows homology to Genbank Acc. No U07971 j
  • RNU07971 Rattus norvegicus Sprague-Dawley L-arginine: glycine amidinotransferase mRNA, partial cds with an identity of 85%.
  • Clone 50 shows homology to DDBJ Acc.
  • No AU035342 j AU035342 Sugano mouse brain mncb Mus musculus cDNA clone MNCb-0343, mRNA sequence [Mus musculus] with an identity of 87% and to Genbank
  • Clone 51 shows homology to Genbank Acc. No M22998
  • MUSGLUTRN: MUSGLUTRN Mouse facilitated glucose transport protein mRNA, complete cds with an identity of 93% as well as Genabnk Acc. No S77924J S77924: Glut-1 glucose transporter isoform 1 [mice, embryo, mRNA partial, 321 nt] with an identity of 93%.
  • Clone 52 shows homology to EMBL Acc. No AJ010953
  • HSA010953 Homo sapiens mRNA for putative Ca2 + -transporting ATPase, partial with an identity of 79%.
  • Clone 53 shows homology to Acc. No U94593: Mus muscle uncoupling protein homolog (UCPH) mRNA.
  • Clone 54 shows homology to Acc. No M2731 5.1: Rattus norvegicus mitochondrial cytochrome c oxidase subunits I, II and III, and ATPase subunit 6 genes, complete cds.
  • Clone 55 is composed of the T7 and BGH sequences. The total sequence is given under total sequence.
  • Clone 12 shows homology to the following database entries: Acc. No AL080317.1 1: Human DNA sequence from clone RP5-1 1 12D6 on chromosome 6q21-22.2. Contains the gene for a PUTATIVE novel protein similar to bacterial NARK (nitrite extrusion protein, nitrite facilitator), the 3 'end of the REV3L gene for REV3 (yeast homologHike, catalytic subunit of DNA polymerase zeta (EC 2.7.7.7, POLZ), ESTs, STSs, GSSs and a putative CpG island, complete sequence.
  • Clone 56 shows homology to Acc. No AW106096.1: um23a10.y1 Sugano mouse embryo mewa Mus musculus cDNA clone 1MAGE: 2225370 5 ', mRNA sequence.
  • Clone 57 shows homology to Acc. No NM_000210.1 Homo sapiens integrin, alpha 6 (ITGA6) mRNA.
  • Clone 58 shows homology to Acc. No AW106096. 1: um23a 10.y1 Sugano mouse embryo mewa Mus musculus cDNA clone IMAGE: 2225370 5 ', mRNA sequence.
  • Clone 59 shows homology to Acc. No NM_007748.1: Mus muscle cytochrome c oxidase, subunit VI a, polypeptide 1 (Cox6a1), mRNA.
  • Clone 60 shows homology to Acc. No AC005403.1: Mus musclulus clone UWGC: ma53a068 from 14D1 -D2 (T-Cell Receptor Alpha Locus), complete sequence.
  • Clone 61 shows homology to Acc. No L07095.1: Mus domesticus strain NZB / B1 NJ mitochondrion genome, complete sequence.
  • Clone 62 shows homology to Acc. No NM_0091 55.1: Mus muscle selenoprotein P, plasma, 1 (SeppD, mRNA complete cds.
  • Clone 63 shows homology to Acc. No J03297.1: Mouse ERp99 mRNA encoding an endoplasmic reticulum transmembrane protein.
  • Clone 64 shows homology to Acc. No AF047431 .1: Homo sapiens AAPT1-Iike protein mRNA, partial cds.
  • Clone 65 shows homology to EMBL Acc. No U79287 Human clone 23867 mRNA sequence 1 396 bp.
  • Clone 66 shows homology to Acc. No C88489, AA073437, AI048028 and to ESTs with the DDBJ Acc. No C88489; Genbank Acc. No AA073437; AI048028.
  • Clone 67 shows homology to Acc. No AK001441: Homo sapiens cDNA FLJ 10579 fis, clone NT2RP2003446 2251 bp mRNA and to ESTs with the Acc. No AI663355 and AU080732.
  • Clone 68 shows homology to Acc. No AF005038: Homo sapiens Secretory Carrier Membrane Protein (SCAMP2) mRNA
  • Clone 69 shows homology to Acc. No U44731: Putative purine nucleotide binding protein mRNA muscle.
  • Clone 71 shows homology to Acc. No NM_004256: Homo sapiens organic cationic transporter-like 3 (ORCTL3) mRNA.
  • Clone 72 shows homology to Acc. No AK000559: Homo sapiens cDNA FLJ20552 fis, clone KAT1 1 732.
  • Clone 73 shows homology to Acc. No X89968: Rattus norvegicus mRNA for alpha-soluble NSF attachment protein.
  • Clone 74 shows homology to Acc. No D851 37: Mouse mRNA for PPI gamma (protein phosphatasel gamma).
  • Clone 75 shows homology to Acc. No NM_001089: Homo sapiens ATP-binding cassette, sub-family A (ABC1), member 3 (ABCA3) mRNA.
  • Clone 76 shows homology to mouse ESTs with the Acc. No AI315969, AI930239, A1666299 and AV141 103.
  • Clone 77 shows homology to Acc. No AB025405: Mus musculus mRNA for sid2895p (Mouse microsomal signal peptidase)
  • Clone 78 shows homology to Acc. No AK000427.
  • the clone is partially homologous to: Homo sapiens cDNA FLJ20420 fis, clone KAT02462.
  • Clone 79 shows homology to Acc. No NM_009127: Mus musculus stearoyl-Coenzyme A desaturase 1 (Scd1)
  • Clone 80 shows homology to Acc. No U21049: Human DD96 mRNA (a gene selectively upregulated in human carcinomas).
  • Clone 81 shows homology to Acc. No AF070626 or AB020980: Homo sapiens clone 24483 unknown mRNA (AF070626), or Homo sapiens mRNA for putative membrane protein (AB020980).
  • Clone 82 shows partial homology to Acc. No U92989: Homo sapiens clone DT1 P1 E1 1 mRNA, CAG repeat region.
  • Clone 83 shows homology to Acc. No AF035208: Mus muscle putative v-SNARE Vtil b mRNA (soluble NSF attachment protein receptor).
  • Clone 84 shows homology to Acc. No NM_012504: Rattus norvegicus ATPase, Na + K + transporting, alpha 1 polypeptide (Atp1 a1), mRNA.
  • Clone 85 shows homology to Acc No NM_007749: Mus musculus cytochrome c oxidase subunit Vllc (Cox7c), mRNA.
  • Clone 86 shows homology to Acc No AF079565: Mus ubiquitin-specific protease UBP41 (Ubp41) mRNA.
  • Clone 87 shows homology to Acc No AJ006341: Mus musculus mRNA for peroxisomal integral membrane protein PMP34.
  • Clone 88 shows homology to Acc No AF223950: Mus musculus TIM22 preprotein translocase (Tim22) mRNA, complete cds; nuclear gene for mitochondrial product.
  • Clone 89 shows homology to Acc No NM_01 1479: Mus muscle serine palmitoyltransferase, long chain base subunit 2 (Sptlc2), mRNA.
  • Clone 90 shows homology to Acc No M23984: Rat mitochondrial proton / phosphate symporter mRNA, complete cds and Acc. No X60036: H. sapiens mRNA for mitochondrial phosphate carrier protein.
  • Clone 91 shows homology to Acc No AF1 13127: Homo sapiens S1 R protein (S1 R) mRNA, complete cds (human cowpox virus homolog).
  • Clone 92 is composed of the T7 and BGH sequences. The entire sequence is shown as a merging of several EST sequences (“EST assembly”) in Contig. Clone 92 shows homology to UniGene Mm.86545.
  • Clone 93 shows homology to human genomic DNA (PAC 69E1 1 on chromosome 1 q23-24).
  • Clone 94 shows homology to Acc No NM_01 1512: Mus musculus surfeit gene 4 (Surf4), mRNA.
  • Clone 95 is composed of the T7 and BGH sequences. The entire sequence is shown as a combination of several EST sequences (“EST assembly”) under total cDNA EST clusters. Clone 95 shows homology to UniGene: Mm.27841 (mouse) and Hs.100132 (human).
  • Clone 96 shows homology to UniGene Hs.133494, Mm.31778 and Rn.1 1778.
  • Clone 97 shows homology to Acc No AF192558: DEFINITION Mus musculus domesticus mitochondrial carrier homolog 1 isoform b (Mtchl) mRNA, complete cds; nuclear gene for mitochondrial product and Acc.
  • No AF192558 / AF192559 Homo sapiens mitochondrial carrier homolog 1 isoform b (MTCH1) mRNA, partial cds; nuclear gene for mitochondrial product.
  • Clone 98 shows homology to Acc No NM_016783: Mus musculus progesterone receptor membrane component (Pgrmc-pending), mRNA.
  • Clone 99 shows homology to Acc No NM_003002: Homo sapiens succinate dehydrogenase complex, subunit D, integral membrane protein (SDHD) mRNA.
  • Clone 100 shows homology to Acc No U95822: Human putative transmembrane GTPase mRNA, partial cds.
  • Clone 101 shows homology to Acc No X99963: M. musculus rhoB gene.
  • Clone 102 shows homology to Acc No AF161 525: Homo sapiens HSPC177 mRNA, complete cds.
  • the gene contains a BH3 domain that is found in many Bcl-2-like genes. Genes with such "BH3-only" domains are pro-apoptotic and have the evolutionarily conserved function of inducing apoptosis. The experiments showed that CGl-135 with Bcl-2 gene family members can interact.
  • the BH3 domain is necessary for efficient apoptosis induction by CGl-135.
  • Clone 103 shows no homology to known sequences.
  • Clone 104 shows homology to Unigene Acc No AW682500.30119 Mm.30119jMm.30119: Mus musculus cDNA with an identity of 91%.
  • Clone 105 shows homology to EMBL Acc No X98475.1 jMMVASP: M. musculus VASP gene with an identity of 90%.
  • Clone 106 shows homology to EMBL Acc No X03369.1
  • RNTUBB15 Rat mRNA for beta-tubulin T beta15 with an identity of 90%.
  • Clone 107 shows homology to Genbank Acc No BE226644.1 JBE226644: ia23h11.y1 Mouse E10 5 12 5 Pancreas cDNA Library Mus musculus cDNA 5 'similar to SW: NUML_BOVIN Q01321 NADH-UBIQUINONE OXIDOREDUCTASE MLRQ SUBUNIT with an identity of 87%.
  • Clone 108 shows homology to Genbank Acc No AF157317.1
  • AF157317 Homo sapiens AD-015 protein mRNA, complete cds with an identity of 83%.
  • Clone 109 shows homology to Genbank Acc No M88136.1
  • CRUSTSTA Cricetulus griseus seryl tRNA synthetase mRNA, partial cds with an identity of 91%.
  • Clone 110 lacks homology to known sequences.
  • Clone 111 shows homology to Genbank Acc No AW106096.27359 j Mm.27359: um23a10.y1 Mus musculus cDNA, 5 'end with an identity of 99%.
  • Clone 112 shows homology to Genbank Acc No AI413025.1
  • Clone 113 shows homology to EMBL Acc No Human DNA sequence from clone RP3-402G11 on chromosome 22q13.31 -13.33 "Contains the MAPK12 gene for mitogen activated protein kinase 12 (SAPK3), the MAPK11 gene for mitogen activated protein kinase 11 (PRKM11) gene KIAA0315, the gene for a novel protein "with an identity of 86%.
  • SAPK3 mitogen activated protein kinase 12
  • PRKM11 mitogen activated protein kinase 11
  • Clone 114 shows homology to DDBJ Acc No AK000823.11 AK000823 Homo sapiens cDNA FLJ20816 fis, clone ADSE00693 with an identity of 88%.
  • Clone 115 shows homology to Genbank Acc No AF007152.11 AF007152 Homo sapiens clone 23649 and 23755 unknown mRNA, partial cds with an identity of 83%.
  • Clone 116 shows homology to EMBL Acc No X66370.1 JRNRPS9 R. norvegicus mRNA for ribosomal protein S9 with an identity of 94%.
  • Clone 117 shows homology to Genbank Acc No BE161116.11 BEI 61116 PM3-HT0424-170200-001-b 11 HT0424 Homo sapiens cDNA with an identity of 100%.
  • Clone 118 shows homology to DDBJ Acc No AK000559.1 JAK000559 Homo sapiens cDNA FLJ20552 fis, clone KAT11732 with an identity of 86%.
  • Clone 119 shows homology to RefSeq Acc No NM_003713.1
  • Clone 120 shows homology to Genbank Acc No M30773.1 (HUMCNR: Human calcineurin B mRNA, complete cds with an identity of 88%.
  • Clone 121 shows homology to RefSeq Acc No NM_013770.1 or Genbank Acc. No AF188712.KAF188712: Mus muscle solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 10 (Slc25a10), mRNA and Mus muscle mitochondrial dicarboxylate carrier mRNA, complete cds; nuclear gene for mitochondrial product with an identity of 98%.
  • Clone 122 shows homology to RefSeq Acc No NM_008525.1: Mus musculus delta-aminolevulinate dehydratase (Lv), mRNA with an identity of 100%.
  • Clone 123 shows homology to Genbank Acc No M59861.
  • KRAT10HCO Rattus norvegicus 10 formyltetrahydrofolate dehydrogenase mRNA, complete cds with an identity of 93%.
  • Clone 124 shows homology to the following database entries: EMBL Acc No X00525.1 (MMRNA02: Mouse 28S ribosomai RNA with an identity of 84%; Genbank Acc. No AF061799.KAF061799: Hydrolagus colliei internal transcribed spacer 1, partial sequence; 5.8S ribosomai RNA gene and internal transcribed spacer 2, complete sequence; and 28S ribosomai RNA gene, partial-sequence with an identity of 83%; Genbank Acc. No AF061800.1 (AF061800: Squalus acanthias internal transcribed spacer 2; and 28S ribosomai RNA gene, partial sequence with an identity of 83%.
  • Clone 125 shows homology to RefSeq Acc. No NM_007469.1: Mus muscle apolipoprotein Cl (Apod), mRNA as well
  • AK011358.1 AK011358 Mus musculus 10 days embryo cDNA, RIKEN full-length enriched library, clone: 261 0009E08, fill insert sequence with an identity of 1 00%.
  • Clone 1 27 shows homology to Genbank Acc. No AK007676: Mus musculus 10 day old male pancreas cDNA, RIKEN full-length enriched library, clone: 1 81 0032P22, fill insert sequence with an identity of 1 00%; to Genbank Acc. No BC002242: Mus musculus, Similar to sorting nexin 5, clone MGC: 7534, mRNA, complete cds with an identity of 100% and Genbank Acc. No AC008066: Homo sapiens BAC clone RP1 1 -293D9 from 8, complete sequence.
  • Clone 1 33 shows homology to Genbank Acc. No AK01 5068.1 J AK01 5068: Mus musculus adult male testis cDNA, RIKEN full-length enriched library, clone: 4930403O06, fill insert sequence with an identity of 90% and to DDBJ Acc. No AK004504.1 1 AK004504: Mus musculus 18 days embryo cDNA, RIKEN full-length enriched library, clone: 1 1 90006A08, fill insert sequence
  • Clone 1 34 shows homology to Genbank Acc. No BC002108.1 ⁇ BC002108: Mus musculus, Similar to ribosomai protein
  • Clone 140 shows homology to DDBJ Acc. No AK008346.1
  • Clone 141 shows no homology to known sequences.
  • Clone 1 44 shows homology to Genbank Acc. No AF006482.1 J AF006482 Mus nucleoside triphosphatase muscle (NTPase) mRNA, complete cds with 99% identity; to Genbank Acc. No AF084569. 1
  • AF084569 Mesocricetus auratus cph proto-oncogene product (cph) mRNA, complete cds with an identity of 90%.
  • Clone 145 shows homology to DDBJ Acc. No AK002787.1 J AK002787 Mus musculus adult male kidney cDNA, RIKEN full-length enriched library, clone: 0610038B1 2, fill insert sequence with an identity of 1 00%; to DDBJ Acc. No AK010989.1
  • Clone 146 shows homology to Refseq Acc. No NM_025278.1
  • apoptosis-inducing genes identified by the genetic screen are listed in Table 1 (pages 1 - 125) attached as an illustration:
  • Table 1 The information in Table 1 is defined as follows:
  • BLAST Altschul et al. (1,997), Nucleic Acids Res. 25: 3389-3402).
  • partially identical sequences identity of preferably ⁇ 85% are also given, which show allelic variants of the specifically shown sequence or homologous sequences from other species, in particular from humans.
  • PSORT II (Nakai, K. and Kanehisa, M., Genomics 14: 897-91 1 (1 992)) determines possible properties and the localization in the resulting peptide from the sequence Cell with a predicted probability.
  • PSORT II (Nakai, K. and Kanehisa, M., Genomics 14: 897-91 1 (1 992)) determines possible properties and the localization in the resulting peptide from the sequence Cell with a predicted probability.
  • PSORT II (Nakai, K. and Kanehisa, M., Genomics 14: 897-91 1 (1 992)
  • PI-Facs Propidium iodide-Facs “: Detection for apoptosis (Bitzer et al. (1999) J. Virol. 73: 702-708)
  • apoptosis-induced genes identified by the genetic screen (clones 1-124, 125, 127, 133, 134, 140, 141, 144, 145, 146) are listed in Table 2 (pages 1-28) attached as an illustration.
  • BGH sequence AAATATCACA GGGCCNTGNT AGCCTGCTNT TCCCTCTTCT TCTTCTTCTA NTGGAANCCG AAGNTACCAT NNCGNTTCTT ANCAGTTTCT CCTTTCGGCC NCCNACCCAG TGGCATGGAA GAGAGCGAGG CAGCTGGCTT GAATTCCCAT CTTCTGGGGA ACCTTTGCAG CCTGCAGGGG CCTGGCCATC TTCTTTGCGA CATTTCTTAC AGCCTGGANC CATGATCGTG CTGAGCAACA TTGGCAGCCT GGTCTCATGT TTCTGG TACTTTTTGA CAAGAGCCCT CTTTGTCTCT GGATCGCGAC TTCTGTGTAT GGAGCCTCAA TGGCAGCCAC GTTTCCCAGC GGCATCTCCT GGATTGAGCA GTACNCCNCC TTAACTGGGA AATCTGCAGC ATTCTTTGTA ATTGGCTCTG CCCTGGGAGA TATGGCCATTCCAGCGGTGA TCGGAATTCT
  • Running clone number # 13 ACCESS No .: AL080317.11 G ⁇ .5830430 Definition: Human DNA sequence from äone RP5-1 12D6 on chromosome 6q2 -22.2. Contains the gene for a PUTATIVE novel protein similar to bacterial NARK (nitrite extrusion protein, nitrite facilitator), the 3 'end of the REV3L gene for REV3 (yeast homolog) -like, catalytic subunit of DNA polymerase zeta (EC 2.7.7.7, POLZ), ESTs, STSs, GSSs and a putative CpG island, complete sequence.
  • Running clone number # 20 (EST) ACCESS No .: AW106096.1 Gl: 6076832 Definition: um23a10.y1 Sugano mouse embryo mewa Mus musculus cDNA clone
  • BGH sequence GACCTGGCTT TNGCNCGCNN TNCANCCCTA GTCCCCCGGG TTCTCACTCC CTAAGCCCAT CGCAGNCCGG NTNGTGGANC CGCGCGTCCC AGGNTTCGTC CTTNCNCGGC CTNCAAGAAC ATGGCTTGCT TCAGAAAGAA AATAGTTTTG TCTTCTCTAA NAACTTACNT TCAGCTTGTC GAAGATGAAA ATAAAAAGCC CTGGAGAGGA ATAATTTCTT GCNCTTTATG AATCTATTTT TAAAATAAAA AAATTTACCN NCTTTNAATC TTTTTCCTCC TCNCAAAAGN AACCAGTATT TTTGCCTNCC ATTCANTTTG CNNCANTAAG ANNTTTGGAG CCTGAAACCN NAGNCTTTNT NANGGANTNT CNCCTTGGTT CAGCCTGNAG GCAAATCTGA TCAACGGACC TTTATGAGTC ATTTTTCCTA GACATATTCA GAAAACCTAG GAGCTGTGTC AAATGCCTGA ATT
  • LOCUS Mus musculus selenoprotein P, plasma, 1 (Seppl), mRNA complete cds.
  • BGH sequence TTTTTTTTTTNTNGATATTAGNTANGTTTTATTATTTNTTATCTNTATGAGGAAGGGGTATCCCAGACAGGGAGACTGNTGAG
  • Running clone number UI-14 ACCESS No .: NM_004256 Definition: Homo sapiens organic cationic transporter-like 3 (ORCTL3) mRNA.
  • ORCTL3 Homo sapiens organic cationic transporter-like 3
  • T7 sequence TTCACCCCGCTTGGTACCGAGCTCGGATCCCTAGTAACGGCCGCCAGTGTGCTGGAAAGATGGAGCCTGCGTTTCACAGA

Abstract

The invention relates to novel apoptosis-associated and especially apoptosis-inducing nucleic acid sequences, polypeptides which are coded by said sequences, and the use of the same for preparing diagnostic and therapeutic agents. The invention also relates to cell systems, transgenic animals and the use thereof for genetic and/or pharmacological research into apoptosis-associated illnesses.

Description

Apoptose-induzierende DNA-SequenzenApoptosis-inducing DNA sequences
Beschreibungdescription
Die Erfindung betrifft neue Apoptose-assoziierte und insbesondere Apoptose-induzierende Nukleinsäuresequenzen, davon kodierte Polypeptide und deren Verwendung zur Bereitstellung diagnostischer und therapeutischer Mittel. Zusätzlich betrifft die Erfindung transgene Zellsysteme sowie Tiere und deren Verwendung zur genetischen und/oder pharmakologischen Untersuchung von Apoptose-assoziierten Krankheiten.The invention relates to new apoptosis-associated and in particular apoptosis-inducing nucleic acid sequences, polypeptides encoded thereof and their use for the provision of diagnostic and therapeutic agents. In addition, the invention relates to transgenic cell systems and animals and their use for the genetic and / or pharmacological investigation of apoptosis-associated diseases.
Apoptose ist das genetisch kodierte Selbstmordprogramm, welches in eukaryontischen Zellen unter bestimmten physiologischen oder pathologischen Bedingungen induziert wird. Die Induktion der Apoptose muss außerordentlich präzise reguliert sein, denn eine Hyperaktivität kann zu degenerativen Erkrankungen führen. Auf der anderen Seite kann eine verringerte Apoptose-Induktion zur Tumorprogression beitragen.Apoptosis is the genetically encoded suicide program that is induced in eukaryotic cells under certain physiological or pathological conditions. The induction of apoptosis must be regulated extremely precisely because hyperactivity can lead to degenerative diseases. On the other hand, reduced apoptosis induction can contribute to tumor progression.
Verschiedene niedermolekulare Induktoren der Apoptose wurden bereits beschrieben. Eine wichtige Klasse sind Tumorcytostatika. Auf welche Weise diese Cytostatika oder andere Substanzen Apoptose induzieren können, ist in den meisten Fällen jedoch unbekannt.Various low-molecular inducers of apoptosis have already been described. An important class is tumor cytostatics. How these cytostatics or other substances can induce apoptosis is, however, in most cases unknown.
Die Identifizierung von Apoptose-induzierenden Genen oder anderen dominanten Genen mit einer nicht-selektionierbaren Aktivität ist problematisch, da eine stabile rekombinante Expression solcher Gene in einer Zielzelle entweder gar nicht oder nur sehr schwer möglich ist. Daher ist es erforderlich, spezielle Screening-Verfahren zur Identifizierung solcher Gene zu verwenden. Hierzu wurden bereits verschiedene in vitro Verfahren entwickelt (King et al., Science 277 ( 1 997), 973-974 und Lustig et al., Meth. Enzymol. 283 ( 1 997), 83-99). Von anderen Arbeitsgruppen wurden transgene Mäuse erzeugt, die multiple Transgene enthalten, deren Funktionen durch Untersuchung des Phänotyps bestimmt wird (Simonet et al., Cell 89 (1997), 309-319 und Smith et al., Nat. Genet. 16 (1997), 28- 36). Ein Nachteil bei den in vitro Verfahren besteht darin, dass die erhaltenen Ergebnisse nicht ohne weiteres mit komplex regulierten zellbiologischen Effekten korrelieren. Untersuchungen an transgenen Tiere wiederum sind sehr aufwendig und mühsam.The identification of apoptosis-inducing genes or other dominant genes with a non-selectable activity is problematic since stable recombinant expression of such genes in a target cell is either not possible or is very difficult. It is therefore necessary to use special screening methods to identify such genes. Various in vitro methods have already been developed for this (King et al., Science 277 (1 997), 973-974 and Lustig et al., Meth. Enzymol. 283 (1 997), 83-99). From other working groups transgenic mice containing multiple transgenes, the functions of which are determined by examining the phenotype (Simonet et al., Cell 89 (1997), 309-319 and Smith et al., Nat. Genet. 16 (1997), 28-36 ). A disadvantage of the in vitro methods is that the results obtained do not readily correlate with complex regulated cell biological effects. In turn, studies on transgenic animals are very complex and tedious.
Grimm und Leder (J. Exp. Med. 185 (1997), 1 137-1 142) beschreiben ein Verfahren zur Identifizierung und Isolierung dominanter Apoptose- induzierender Nukleinsäuresequenzen. Hierbei werden kleine Plasmidpools entsprechend 20 Klonen aus normalisierten cDNA-Expressionsbibliotheken in die humane Nierenzellinie 293 transient eingeführt. Die Apoptose- induzierende Aktivität einer Nukleinsäuresequenz wird manuell durch mikroskopische Inspektion auf für Apoptose charakteristische morphologische Merkmale bestimmt. Mit Hilfe dieses Verfahrens konnte das Apoptose-induzierende Adenin-nukelotid-Translokase-1-(ANT-1 ) Gen identifiziert werden. Das ANT-1 -Gen gilt als ursächlich für die degenerative Herzkrankheit dilatorische Kardiomyopathie (DCM) (PCT/EP00/08812).Grimm and Leder (J. Exp. Med. 185 (1997), 1 137-1 142) describe a method for identifying and isolating dominant apoptosis-inducing nucleic acid sequences. Small plasmid pools corresponding to 20 clones from normalized cDNA expression libraries are transiently introduced into the human kidney cell line 293. The apoptosis-inducing activity of a nucleic acid sequence is determined manually by microscopic inspection for morphological features characteristic of apoptosis. The apoptosis-inducing adenine nucleotide translocase 1 (ANT-1) gene was identified using this method. The ANT-1 gene is considered to be the cause of the degenerative cardiac disease dilatory cardiomyopathy (DCM) (PCT / EP00 / 08812).
In dem beschriebenen Verfahren werden zur Transfektion in die Nierenzelllinie 293 Plasmidpools von 20 Klonen verwendet. Durch die Transfektion von Plasmidpools kann für das Screeningverfahren jedoch nur eine geringe Sensitivität erreicht werden, da diejenigen Expressionsplasmide, die Apoptose-induzierende Gene beinhalten, in jeweils geringem Verhältnis zu den Expressionsplasmiden vorliegen, die keine Apoptose-induzierenden Gene tragen. Um dieses Problem zu umgehen, wurden in dem hier eingesetzten Screeningverfahren zur Transfektion statt Plasmidpools Aliquots verwendet, die statistisch nur einen Klon enthielten. Zusätzlich wurde eine besonders effektive Art zur Aufreinigung von Plasmid-DNA verwendet (Neudecker und Grimm, Biotechniques 28 (2000), 107-109). Dieses Verfahren ermöglicht die Reduktion von Lipopolysacchäriden um den Faktor 900 gegenüber bisher publizierten Methoden und ermöglicht so erstmals die Aufreinigung von Plasmid-DNA für den Hochdurchsatz im "96 well"-Format. Durch den Einastz besonders aufgereinigter DNA zur Transfektion in Kombination mit der Verwendung von einzelnen Klonen statt Plasmidpools konnte ein besonders effektives und sensitives Screeningverfahren durchgeführt werden, das es ermöglichte, eine große Anzahl Apoptose-induzierender Sequenzen zu ermitteln.In the described method 293 plasmid pools of 20 clones are used for transfection into the kidney cell line. The transfection of plasmid pools, however, can only achieve a low sensitivity for the screening method, since those expression plasmids which contain apoptosis-inducing genes are present in each case in a low ratio to the expression plasmids which do not carry apoptosis-inducing genes. In order to avoid this problem, aliquots which contained only one clone statistically were used instead of plasmid pools in the screening method used for transfection. In addition, a particularly effective way of purifying plasmid DNA was used (Neudecker and Grimm, Biotechniques 28 (2000), 107-109). This procedure enables Reduction of lipopolysaccharides by a factor of 900 compared to previously published methods and thus enables for the first time the purification of plasmid DNA for high throughput in the "96 well" format. The use of specially purified DNA for transfection in combination with the use of individual clones instead of plasmid pools enabled a particularly effective and sensitive screening process to be carried out, which made it possible to determine a large number of apoptosis-inducing sequences.
Ein Gegenstand der vorliegenden Erfindung sind neue Apoptose-assoziierte und insbesondere Apoptose-induzierende Nukleinsäuren umfassend: (a) die in Tabelle 1 , in Tabelle 2 oder in SEQ ID NO: 1 -225 gezeigten Nukleinsäuren der Klone 1 - 1 6, dazu komplementäre Nukleinsäuren oder Fragmente davon, (b) den Sequenzen gemäß (a) im Rahmen der Degeneration des genetischen Codes entsprechende Nukleinsäuren und (c) mit den Sequenzen gemäß (a) oder/und (b) unter stringenten Bedingungen hybridisierende Nukleinsäuren.The present invention relates to new apoptosis-associated and in particular apoptosis-inducing nucleic acids comprising: (a) the nucleic acids of clones 1 - 1 6 shown in Table 1, in Table 2 or in SEQ ID NO: 1 - 225, and nucleic acids complementary thereto or fragments thereof, (b) nucleic acids corresponding to the sequences according to (a) in the context of the degeneration of the genetic code and (c) nucleic acids hybridizing with the sequences according to (a) or / and (b) under stringent conditions.
Die erfindungsgemäßen Nukleinsäuren sind Apoptose-assoziierte Nukleinsäuren, d.h. Nukleinsäuren, die mit dem Auftreten apoptotischer Prozesse in einer Zelle, insbesondere in einer Säugerzelle, assoziiert sind. Vorzugsweise sind die Nukleinsäuren Apoptose-induzierende Nukleinsäuren, d.h. Nukleinsäuren, die apoptotische Prozesse hervorrufen oder/und fördern können. Besonders bevorzugt sind die erfindungsgemäßen Nukleinsäuren dominant Apoptose-induzierende Nukleinsäuren, die in der Lage sind, bei Expression in einer Zelle Apoptose zu induzieren und die für Apoptose charakteristischen Merkmale, wie etwa DNA-Fragmentierung, morphologische Besonderheiten etc., hervorzurufen. Die Nukleinsäuren können in doppelsträngiger oder einzelsträngiger Form, z.B. als DNA oder RNA, vorliegen. Die isolierten Nukleinsäuren können ihren zellulären Effekt durch Expression, insbesondere durch Überexpression in Zellen entfalten. Damit sind sie induzierbar und ihre Verwendung als therapeutisches Agens definiert.The nucleic acids according to the invention are apoptosis-associated nucleic acids, ie nucleic acids which are associated with the occurrence of apoptotic processes in a cell, in particular in a mammalian cell. The nucleic acids are preferably apoptosis-inducing nucleic acids, ie nucleic acids which can induce and / or promote apoptotic processes. The nucleic acids according to the invention are particularly preferably dominant apoptosis-inducing nucleic acids which are capable of inducing apoptosis when expressed in a cell and which produce the characteristics characteristic of apoptosis, such as DNA fragmentation, morphological peculiarities etc. The nucleic acids can be in double-stranded or single-stranded form, for example as DNA or RNA. The isolated nucleic acids can develop their cellular effect by expression, in particular by overexpression in cells. This makes them inducible and defines their use as a therapeutic agent.
Neben den in Tabelle 1 bzw. den entsprechenden Sequenzprotokollen gezeigten Nukleinsäuren oder Teilfragmenten davon mit einer Länge von vorzugsweise mindestens 1 5, besonders bevorzugt mindestens 20 und am meisten bevorzugt mindestens 25 Nukleotiden, werden auch Varianten dieser Sequenzen von der vorliegenden Erfindung erfaßt. Neben den Nukleinsäuren, die den Sequenzen gemäß (a) im Rahmen der Degeneration des genetischen Codes entsprechen und für ein Polypeptid mit der gleichen Aminosäuresequenz codieren, werden auch Nukleinsäuren erfasst, die mit den Sequenzen gemäß (a) oder/und (b) unter stringenten Bedingungen hybridisieren. Hybridisierung unter stringenten Bedingungen bedeutet im Rahmen der vorliegenden Anmeldung, dass nach Vorhybridisierung und Hybridisierung bei geeigneten Bedingungen und Waschen in 1 x SSC und 0, 1 % SDS bei 55 °C, vorzugsweise bei 62 °C und besonders bevorzugt bei 68 °C und insbesondere in 0,2 x SSC und 0, 1 % SDS bei 55 °C, vorzugsweise bei 62 °C und besonders bevorzugt bei 68 °C noch ein Hybridisierungssignal gefunden wird (siehe auch Sambrook et al., Molecular Cloning. A Laboratory Manual (1 989), Cold Spring Harbor Laboratory Press, 1 .101 -1 .104).In addition to the nucleic acids or partial fragments thereof shown in Table 1 or the corresponding sequence protocols, with a length of preferably at least 15, particularly preferably at least 20 and most preferably at least 25 nucleotides, variants of these sequences are also covered by the present invention. In addition to the nucleic acids which correspond to the sequences according to (a) in the context of the degeneration of the genetic code and which code for a polypeptide with the same amino acid sequence, nucleic acids are also recorded which are stringent with the sequences according to (a) or / and (b) Hybridize conditions. In the context of the present application, hybridization under stringent conditions means that after pre-hybridization and hybridization under suitable conditions and washing in 1 × SSC and 0.1% SDS at 55 ° C., preferably at 62 ° C. and particularly preferably at 68 ° C. and in particular a hybridization signal is also found in 0.2 x SSC and 0.1% SDS at 55 ° C, preferably at 62 ° C and particularly preferably at 68 ° C (see also Sambrook et al., Molecular Cloning. A Laboratory Manual (1 989), Cold Spring Harbor Laboratory Press, 1 .101 -1 .104).
Die erfindungsgemäßen Apoptose-assoziierten Nukleinsäuren codieren vorzugsweise für ein Apoptose-assoziiertes Polypeptid oder ein funktionelles Fragment davon. Die Nukleinsäuren können von einem beliebigen Organismus stammen, wobei eukaryontische Organismen wie Nematoden, z.B. C. elegans, Arthropoden wie Drosophila, Cordata und Wirbeltiere, z.B. Säuger, bevorzugt sind. Besonders bevorzugt handelt es sich um Sequenzen von Säugern, z.B. von der Maus oder vom Menschen, wobei diese Sequenzen gegebenenfalls noch durch bekannte molekularbiologische Techniken, wie etwa ortsspezifische Mutagenese, PCR, Restriktionsspaltung und Ligation, verändert werden können. Die erfindungsgemäßen Nukleinsäuren liegen vorzugsweise in operativer Verknüpfung mit einer Expressionskontrollsequenz vor, so dass sie in einer geeigneten Wirtszelle transkribiert und gegebenenfalls translatiert werden können. Expressionskontrollsequenzen umfassen üblicherweise einen Promotor und gegebenenfalls regulatorische Sequenzen wie Operatoren oder Enhancer. Weiterhin können auch Translations-lnitiationssequenzen vorhanden sein. Geeignete Expressionskontrollsequenzen für prokaryontische oder eukaryontische Wirtszellen sind dem Fachmann bekannt (siehe z.B. Sambrook et al., supra).The apoptosis-associated nucleic acids according to the invention preferably code for an apoptosis-associated polypeptide or a functional fragment thereof. The nucleic acids can originate from any organism, eukaryotic organisms such as nematodes, for example C. elegans, arthropods such as Drosophila, Cordata and vertebrates, for example mammals, being preferred. Sequences from mammals, for example from the mouse or from humans, are particularly preferred, these sequences optionally being able to be modified by known molecular biological techniques, such as site-specific mutagenesis, PCR, restriction cleavage and ligation. The nucleic acids according to the invention are preferably operatively linked to an expression control sequence so that they can be transcribed and, if necessary, translated in a suitable host cell. Expression control sequences usually comprise a promoter and optionally regulatory sequences such as operators or enhancers. Translation initiation sequences may also be present. Suitable expression control sequences for prokaryotic or eukaryotic host cells are known to the person skilled in the art (see, for example, Sambrook et al., Supra).
Ein weiterer Gegenstand der Erfindung ist ein rekombinanter Vektor, der eine erfindungsgemäße Nukleinsäure, vorzugsweise in operativer Verknüpfung mit einer Expressionskontrollsequenz enthält. Der rekombinante Vektor kann weiterhin noch übliche Elemente wie einen Replikationsursprung und ein Selektionsmarkergen enthalten. Beispiele für geeignete rekombinante Vektoren, z.B. Plasmide, Cosmide, Phagen, Viren etc., sind dem Fachmann bekannt (siehe z.B. Sambrook et al., supra).Another object of the invention is a recombinant vector which contains a nucleic acid according to the invention, preferably in operative linkage with an expression control sequence. The recombinant vector can also contain common elements such as an origin of replication and a selection marker gene. Examples of suitable recombinant vectors, e.g. Plasmids, cosmids, phages, viruses etc. are known to the person skilled in the art (see e.g. Sambrook et al., Supra).
Noch ein weiterer Gegenstand der Erfindung sind rekombinante Zellen, die mit einer erfindungsgemäßen Nukleinsäure oder einem erfindungsgemäßen Vektor transformiert oder transfiziert sein können. Die Transformation bzw. Transfektion kann nach bekannten Methoden erfolgen, z.B. durch Calciumphosphat-Copräzipitation, Lipofektion, Elektroporation, Partikelbeschuß oder virale Infektion. Die erfindungsgemäße Zelle kann die rekombinante Nukleinsäure in extrachromosomaler oder chromosomal integrierter Form enthalten.The invention further relates to recombinant cells which can be transformed or transfected with a nucleic acid or a vector according to the invention. The transformation or transfection can be carried out according to known methods, e.g. by calcium phosphate coprecipitation, lipofection, electroporation, particle bombardment or viral infection. The cell according to the invention can contain the recombinant nucleic acid in extrachromosomal or chromosomally integrated form.
Noch ein weiterer Gegenstand der Erfindung sind Apoptose-assoziierte Polypeptide, die von einer erfindungsgemäßen Nukleinsäure codiert sind. Apoptose-assoziierte Polypeptide können durch Expression der erfindungsgemäßen Apoptose-assoziierten Nukleinsäuren, durch chemische Synthese oder durch Kombinationen beider Methoden erhalten werden. Noch ein weiterer Gegenstand der Erfindung ist eine pharmazeutische Zusammensetzung, die eine erfindungsgemäße Nukleinsäure, einen erfindungsgemäßen Vektor oder ein erfindungsgemäßes Polypeptid gegebenenfalls zusammen mit pharmazeutisch üblichen Träger- und Hilfsstoffen enthält. Die zuvor beschriebenen Nukleinsäuren, Vektoren, Zellen und Polypeptide können zur Herstellung eines diagnostischen oder therapeutischen Mittels eingesetzt werden, insbesondere eines Mittels zur Diagnose, Therapie oder Prävention von Apoptose-assoziierten Erkrankungen. Apoptose-assoziierte Erkrankungen können sich einerseits durch eine abnorm verringerte Apoptose und somit durch eine Hyperproiiferation auszeichnen, beispielsweise Tumorerkrankungen, Autoimmunerkrankungen und virale Infektionen (Thompson, Science 267 (1995), 1456-1462). Andererseits können Apoptose-assoziierte Erkrankungen sich auch durch eine abnorm erhöhte Apoptose und somit durch degenerative Erscheinungen auszeichnen, wie etwa die Alzheimer Krankheit, Huntington's Disease, Parkinsons Krankheit, Reperfusions- Schäden, Schlaganfall und Alkohol-Schädigungen der Leber (Thompson (1995), supra).Another object of the invention is apoptosis-associated polypeptides which are encoded by a nucleic acid according to the invention. Apoptosis-associated polypeptides can be obtained by expression of the apoptosis-associated nucleic acids according to the invention, by chemical synthesis or by combinations of both methods. Another object of the invention is a pharmaceutical composition which contains a nucleic acid according to the invention, a vector according to the invention or a polypeptide according to the invention optionally together with pharmaceutically customary excipients and auxiliaries. The nucleic acids, vectors, cells and polypeptides described above can be used to produce a diagnostic or therapeutic agent, in particular an agent for the diagnosis, therapy or prevention of apoptosis-associated diseases. Apoptosis-associated diseases can be characterized on the one hand by an abnormally reduced apoptosis and thus by hyperproiiferation, for example tumor diseases, autoimmune diseases and viral infections (Thompson, Science 267 (1995), 1456-1462). On the other hand, apoptosis-associated diseases can also be characterized by abnormally increased apoptosis and thus by degenerative symptoms, such as Alzheimer's disease, Huntington's disease, Parkinson's disease, reperfusion damage, stroke and alcohol damage to the liver (Thompson (1995), supra ).
Die diagnostische Anwendung umfasst einen qualitativen oder/und quantitativen Nachweis der Apoptose-assoziierten Nukleinsäure, z.B. in Form eines Transkripts, oder des davon codierten Polypeptids in einer Probe, insbesondere einer Probe, die einem erkrankten Organismus, beispielsweise einem Patienten, entnommen wurde. Der Nachweis kann auf übliche Art und Weise, z.B. durch Nukleinsäure-Hybridisierung oder -Amplifikationsreaktionen wie etwa PCR oder durch Proteinnachweis über Antikörper, erfolgen. Dem Fachmann sind hierzu zahlreiche Techniken bekannt. Der Nachweis kann auch durch die Verwendung der isolierten Gene auf einem DNA-Chip erfolgen. Dadurch können mehrere, z.B. alle Gene gleichzeitig in einem Experiment untersucht werden. Die therapeutische oder präventive Anwendung umfasst die Verabreichung eines Wirkstoffs an einen erkrankten Organismus in einer ausreichenden Dosierung, um die Apoptose-assoziierte Erkrankung zu lindern oder zu heilen bzw. um den Ausbruch einer Apoptose-assoziierten Krankheit zu verhindern. In einer Ausführungsform der Erfindung wird dabei eine Apoptose-assoziierte Nukleinsäure auf einem gentherapeutischen Vektor, z.B. einem Adenovirus, einem Retrovirus, einem Adeno-assoziierten Virus etc., verabreicht, um in einer erkrankten Zielzelle eine erhöhte Expression der Apoptose-assoziierten Nukleinsäure zu bewirken. Alternativ kann auch eine Antisense-Nukleinsäure, z.B. auf einem gentherapeutischen Vektor oder auch direkt, verabreicht werden, sofern eine Verringerung der Expression der Apoptose-assoziierten Nukleinsäure angestrebt wird. In einer weiteren Ausführungsform der Erfindung können Apoptose-assoziierte Polypeptide oder Modulatoren der Aktivität solcher Apoptose-assoziierter Polypeptide, z.B. Aktivatoren oder Inhibitoren, verabreicht werden. Die Verabreichung der Wirkstoffe erfolgt nach bekannten Methoden wie beispielsweise in der Gentherapie (Anderson, Nature 392 (1998), 25-30) oder der Proteintherapie (Schwarze et al., Science 285 (1999), 1569- 1572) beschrieben.The diagnostic application comprises a qualitative and / or quantitative detection of the apoptosis-associated nucleic acid, for example in the form of a transcript, or the polypeptide encoded thereby in a sample, in particular a sample, which was taken from a diseased organism, for example a patient. The detection can be carried out in the usual way, for example by nucleic acid hybridization or amplification reactions such as PCR or by protein detection using antibodies. Numerous techniques for this are known to the person skilled in the art. The detection can also be done by using the isolated genes on a DNA chip. This allows several, eg all genes to be examined simultaneously in one experiment. The therapeutic or preventive application comprises the administration of an active substance to a diseased organism in a sufficient dosage to alleviate or cure the apoptosis-associated disease or to prevent the onset of an apoptosis-associated disease. In one embodiment of the invention, an apoptosis-associated nucleic acid is administered on a gene therapy vector, for example an adenovirus, a retrovirus, an adeno-associated virus, etc., in order to bring about increased expression of the apoptosis-associated nucleic acid in a diseased target cell. Alternatively, an antisense nucleic acid can also be administered, for example on a gene therapy vector or also directly, provided that the expression of the apoptosis-associated nucleic acid is to be reduced. In a further embodiment of the invention, apoptosis-associated polypeptides or modulators of the activity of such apoptosis-associated polypeptides, for example activators or inhibitors, can be administered. The active substances are administered by known methods, for example in gene therapy (Anderson, Nature 392 (1998), 25-30) or protein therapy (Schwarze et al., Science 285 (1999), 1569-1572).
Die erfindungsgemäßen Nukleinsäuren, Vektoren, Zellen und Polypeptide können schließlich auch zur Identifizierung von neuen Wirksubstanzen für die Therapie oder Prävention von Apoptose-assoziierten Erkrankungen eingesetzt werden. Denkbar ist hier der Einsatz in bekannten zellulären oder mo le kularen Screeni ng assays g eg ebe n enf all s in ei ne m Hochdurchsatzformat. Die Erfindung betrifft auch selbstverständlich die durch Anwendung solcher Screeningverfahren identifizierten Wirkstoffe bzw. davon abgeleitete Substanzen. Die durch den Screen identifizierten Wirksubstanzen sind in der Lage, Signalwege zu aktivieren oder zu inhibieren, die durch die Expression der Nukleinsäuren induziert werden. Ein weiterer Gegenstand der vorliegenden Erfindung sind transgene nichthumane Tiere, die (i) das Gen einer erfindungsgemäßen Nukleinsäure oder das ANT-1 -Gen konstitutiv oder induzierbar überexprimieren, (ii) das endogene Gen einer erfindungsgemäßen Nukleinsäure oder das ANT-1 -Gen in inaktivierter Form enthalten, (iii) das endogene Gen einer erfindungsgemäßen Nukleinsäure oder das ANT-1 -Gen vollständig oder teilweise durch ein mutiertes Gen einer erfindungsgemäßen Nukleinsäure oder ein mutiertes ANT-1 -Gen ersetzt enthalten, (iv) eine konditionale und gewebsspezifische Überexpression oder Unterexpression des Gens einer erfindungsgemäßen Nukleinsäure oder des ANT-1 -Gens aufweisen oder (v) einen konditionalen und gewebsspezifischen Knock-out des Gens einer erfindungsgemäßen Nukleinsäure oder des ANT-1 -Gens aufweisen.Finally, the nucleic acids, vectors, cells and polypeptides according to the invention can also be used to identify new active substances for the therapy or prevention of apoptosis-associated diseases. The use in known cellular or molecular screening assays is conceivable here, in a high throughput format. The invention naturally also relates to the active substances identified by the use of such screening methods or substances derived therefrom. The active substances identified by the screen are able to activate or inhibit signaling pathways which are induced by the expression of the nucleic acids. The present invention further provides transgenic non-human animals which (i) constitutively or inducibly overexpress the gene of a nucleic acid according to the invention or the ANT-1 gene, (ii) the endogenous gene of a nucleic acid according to the invention or the ANT-1 gene in inactivated form Contain form, (iii) contain the endogenous gene of a nucleic acid according to the invention or the ANT-1 gene completely or partially replaced by a mutated gene of a nucleic acid according to the invention or a mutated ANT-1 gene, (iv) conditional and tissue-specific overexpression or underexpression of the gene of a nucleic acid according to the invention or of the ANT-1 gene or (v) have a conditional and tissue-specific knock-out of the gene of a nucleic acid according to the invention or of the ANT-1 gene.
Vorzugsweise kann das transgene Tier zusätzlich ein exogenes Gen einer erfindungsgemäßen Nukleinsäure oder ein exogenes ANT-1 -Gen unter Kontrolle eines die Überexpression erlaubenden Promotors enthalten. Alternativ kann das endogene Gen einer erfindungsgemäßen Nukleinsäure oder das endogene ANT-1 -Gen durch Aktivierung oder/und Austausch des eigenen Promotors überexprimiert werden. Vorzugsweise weist der endogene Promotor des Gens einer erfindungsgemäßen Nukleinsäure oder des ANT- 1 -Gens eine genetische Veränderung auf, die zu einer veränderten Expression des Gens führt. Die genetische Veränderung des endogenen Promotors umfasst dabei sowohl eine Mutation einzelner Basen als auch Deletions- und Insertionsmutationen.The transgenic animal can preferably additionally contain an exogenous gene of a nucleic acid according to the invention or an exogenous ANT-1 gene under the control of a promoter which allows overexpression. Alternatively, the endogenous gene of a nucleic acid according to the invention or the endogenous ANT-1 gene can be overexpressed by activation or / and exchange of the own promoter. The endogenous promoter of the gene of a nucleic acid according to the invention or of the ANT-1 gene preferably has a genetic change which leads to a changed expression of the gene. The genetic modification of the endogenous promoter includes a mutation of individual bases as well as deletion and insertion mutations.
Eine erste Ausführungsform betrifft ein transgenes Tier, das das Gen einer erfindungsgemäßen Nukleinsäure oder das ANT-1-Gen konstitutiv oder induzierbar überexprimiert. Gegebenenfalls kann das eingeführte Gen einer erfindungsgemäßen Nukleinsäure oder das eingeführte ANT-1 -Gen zusätzliche Mutationen aufweisen. Eine zweite Ausführungsform betrifft ein transgenes Tier, welches das endogene Gen einer erfindungsgemäßen Nukleinsäure oder das endogene ANT-1 -Gen in inaktivierter Form enthält. Die Inaktivierung des Gens einer erfindungsgemäßen Nukleinsäure oder des ANT-1 -Gens erfolgt dabei vorzugsweise durch Einführung einer Knock-out-Mutation "mittels homologer Rekombination oder durch Einführung eines Antisense- Konstrukts oder eines RNAi-Kontrukts.A first embodiment relates to a transgenic animal which constitutively or inducibly overexpresses the gene of a nucleic acid according to the invention or the ANT-1 gene. The introduced gene of a nucleic acid according to the invention or the introduced ANT-1 gene can optionally have additional mutations. A second embodiment relates to a transgenic animal which contains the endogenous gene of a nucleic acid according to the invention or the endogenous ANT-1 gene in inactivated form. The inactivation of the gene of a nucleic acid of the invention or of the ANT-1 gene is preferably effected by introducing a knock-out mutation "by homologous recombination or by introducing an antisense construct or an RNAi Kontrukts.
Eine dritte Ausführungsform betrifft ein transgenes Tier, bei dem das endogene Gen einer erfindungsgemäßen Nukleinsäure oder das endogeneA third embodiment relates to a transgenic animal in which the endogenous gene of a nucleic acid according to the invention or the endogenous
ANT-1 -Gen vollständig oder teilweise durch ein mutiertes Gen einer erfindungsgemäßen Nukleinsäure oder ein mutiertes ANT-1-Gen ersetzt ist.ANT-1 gene is completely or partially replaced by a mutated gene of a nucleic acid of the invention or a mutated ANT-1 gene.
Eine vierte Ausführungsform betrifft ein transgenes Tier, welches eine konditionale und gewebsspezifische Überexpression oder Unterexpression des Gens einer erfindungsgemäßen Nukleinsäure oder des ANT-1 -Gens aufweist.A fourth embodiment relates to a transgenic animal which has a conditional and tissue-specific overexpression or underexpression of the gene of a nucleic acid according to the invention or of the ANT-1 gene.
In einer fünften Ausführungsform weist das transgene Tier einen konditionalen und gewebsspezifischen Knock-out des Gens einer erfindungsgemäßen Nukleinsäure oder des ANT-1-Gens auf.In a fifth embodiment, the transgenic animal has a conditional and tissue-specific knock-out of the gene of a nucleic acid according to the invention or of the ANT-1 gene.
Vorzugsweise ist das transgene Tier ein Säugetier, wie etwa ein Nager, z.B. eine Maus. Mäuse haben gegenüber anderen Tieren zahlreiche Vorteile. Sie sind leicht zu halten und ihre Physiologie gilt als Modellsystem für die des Menschen. Die Herstellung solch Gen-manipulierter Tiere ist dem Fachmann hinreichend bekannt und wird nach üblichen Verfahren durchgeführt (Hogan, B., Beddington, R., Costantini, F. und Lacy, E. (1994), Manipulating the Mouse-Embryo; A Laboratory Manual, 2. Aufl., Could Spring Harbor Laboratory, Cold Spring Harbor, NY). Die Erfindung betrifft weiterhin die Verwendung eines solchen transgenen Tiers zur genetischen und/oder pharmakologischen Untersuchung von Krankheiten, die mit übermäßiger oder verminderter bzw. fehlender Expression eines Gens einer erfindungsgemäßen Nukleinsäure oder eines ANT-1 -Gens verbunden sind.The transgenic animal is preferably a mammal, such as a rodent, for example a mouse. Mice have numerous advantages over other animals. They are easy to hold and their physiology is considered a model system for that of humans. The production of such gene-manipulated animals is well known to the person skilled in the art and is carried out by customary methods (Hogan, B., Beddington, R., Costantini, F. and Lacy, E. (1994), Manipulating the Mouse-Embryo; A Laboratory Manual, 2nd ed., Could Spring Harbor Laboratory, Cold Spring Harbor, NY). The invention further relates to the use of such a transgenic animal for the genetic and / or pharmacological examination of diseases which are associated with excessive or reduced or no expression of a gene of a nucleic acid according to the invention or of an ANT-1 gene.
Die erfindungsgemäßen transgenen Tiere können als Modell für die mit dem Gen einer erfindungsgemäßen Nukleinsäure oder ANT-1 -Gen verbundenen Krankheiten bei Menschen oder auch bei Nutztieren dienen. So kann beispielsweise die Auswirkung von Wirkstoffen oder Gentherapien auf den Krankheitsverlauf bestimmt werden. Ebenfalls können die Tiere zur Diagnose bzw. dem frühzeitigen Erkennen einer Krankheit von Nutzen sein.The transgenic animals according to the invention can serve as a model for the diseases associated with the gene of a nucleic acid or ANT-1 gene according to the invention in humans or also in farm animals. For example, the effects of drugs or gene therapies on the course of the disease can be determined. The animals can also be useful for diagnosis or early detection of a disease.
So kann beispielsweise ein erfindungsgemäßes transgenes Tier, welches das ANT-1 -Gen enthält als Modell für die degenerative Herzkrankheit dilatorische Kardiomyopathie (DCM) dienen. Diese degenerative Herzkrankheit ist mit übermäßiger Apoptose in den Herz-Zellen eines Patienten verbunden. Ein erstes Anzeichen, dass der Apoptose-Inducer ANT-1 eine wichtige Rolle bei der Induktion der Apoptose bei der DCM spielt, war die Beobachtung, dass sich bei einem Patienten im Verlauf der DCM bereits sehr früh das Expressionsmuster der ANT-1 -Isoformen im Herzen verschiebt. Es kommt zu einer verstärkten Expression von ANT-1 - mRNA und ANT-1 -Protein (PCT/EP00/0881 2). Zu diesem Zweck kann das ANT-1 -Gen unter Kontrolle des herzspezifischen αr-Myosin Heavy Chain Promoters (Subramaniam, A. (1 991 ), J. Biol. Chem. 266 (36), Seite 24613-24620) in transgenen Mäusen exprimiert werden. Dieser Promoter ist gut charakterisiert und wird erst zum Zeitpunkt der Geburt eingeschaltet. Das Expressionskonstrukt kann beispielsweise hergestellt werden, in dem das ANT-1 -Gen in die Sall-Restriktionsschnittstelle des dritten nicht-kodierenden Exons des 5,5 kB umfassenden Promotors eingefügt wird. Die Herzen der so hergestellten erfindungsgemäßen transgenen Tiere sollten einige der hinsichtlich DCM-spezifischen zellulären Veränderungen, wie Fibrinisierung, Apoptose und Hypertrophie, oder Funktionsstörungen, wie linksventrikulärer Druck, enddiastolischer Druck, Kontraktilität, linksventrikuläre Ausstoßfraktion und linksventrikulärer Fülldruck aufweisen.For example, a transgenic animal according to the invention which contains the ANT-1 gene can serve as a model for the degenerative heart disease dilatoric cardiomyopathy (DCM). This degenerative heart disease is associated with excessive apoptosis in a patient's heart cells. A first indication that the apoptosis inducer ANT-1 plays an important role in the induction of apoptosis in DCM was the observation that the expression pattern of the ANT-1 isoforms in the patient developed very early in the course of DCM Heart shifts. There is an increased expression of ANT-1 mRNA and ANT-1 protein (PCT / EP00 / 0881 2). For this purpose, the ANT-1 gene can be expressed in transgenic mice under the control of the heart-specific αr-myosin heavy chain promoter (Subramaniam, A. (1 991), J. Biol. Chem. 266 (36), pages 24613-24620) become. This promoter is well characterized and is only activated at the time of birth. The expression construct can be produced, for example, by inserting the ANT-1 gene into the Sall restriction site of the third non-coding exon of the 5.5 kB promoter. The hearts of the transgenic animals according to the invention thus produced should contain some of the cellular ones which are specific to DCM Changes such as fibrinization, apoptosis and hypertrophy, or functional disorders such as left ventricular pressure, end diastolic pressure, contractility, left ventricular ejection fraction and left ventricular filling pressure.
Alternativ oder zusätzlich können auch Zellkultursysteme, insbesondere humane Zellkultursysteme, für die Anwendungen eingesetzt werden, die für das transgene Tier beschrieben sind.Alternatively or additionally, cell culture systems, in particular human cell culture systems, can also be used for the applications which are described for the transgenic animal.
Weiterhin soll die Erfindung durch das nachfolgende Beispiel näher erläutert werden.The invention is further illustrated by the following example.
Das Sequenzprotokoll enthält die Sequenzen SEQ ID No. 1 -225, welche die in Tabelle 1 aufgelisteten T7-Sequenzen, BGH-Sequenzen und internen Primer-Sequenzen der indentifizierten Apoptose-induzierenden Gene der Klone 1 -124 umfassen. Tabelle 2 zeigt die Sequenzen der Klone 1 -124 sowie 125, 127, 133, 134, 140, 141 , 144, 145 und 146.The sequence listing contains the sequences SEQ ID No. 1-225, which include the T7 sequences, BGH sequences and internal primer sequences of the identified apoptosis-inducing genes of clones 1-124 listed in Table 1. Table 2 shows the sequences of clones 1-124 and 125, 127, 133, 134, 140, 141, 144, 145 and 146.
Beispiel Isolation von Apoptose-induzierenden GenenExample isolation of apoptosis-inducing genes
1. Allgemeines1. General
Apoptose-induzierende Gene wurden durch einen genetischen Screen in der humanen Zellinie HEK 293T gefunden (Grimm und Leder (1997), supra), der auf der iterativen Transfektion kleiner Expressionsplasmid-Pools aus einer normalisierten Genbibliothek beruht und der anschließenden mikroskopischen Bestimmung des programmierten Zelltodes durch den Phänotyp der apoptotischen Zellen. Die Transfektion von einzelnen Klonen aus einem positiven Plasmid-Pool erlaubt dann, das Apoptose-induzierende Gen zu bestimmen. Dieser Screen wurde in einem 96-Well Format durchgeführt. Desweiteren wurde eine besonders effektive Art, die Plasmid-DNA zu reinigen, verwendet (Neudecker und Grimm, Biotechniques 28 (2000), 107-109).Apoptosis-inducing genes were found by a genetic screen in the human cell line HEK 293T (Grimm and Leder (1997), supra), which is based on the iterative transfection of small expression plasmid pools from a normalized gene library and the subsequent microscopic determination of the programmed cell death the phenotype of the apoptotic cells. The transfection of individual clones from a positive plasmid pool then allows the apoptosis-inducing gene to be determined. This screen was done in a 96-well format. Furthermore, a particularly effective way of purifying the plasmid DNA was used (Neudecker and Grimm, Biotechniques 28 (2000), 107-109).
2. Experimentelle Protokolle2. Experimental protocols
2.1 Zellkultur und Transfektionen2.1 Cell culture and transfections
Humane HEK 293T-Zellen wurden in DMEM ergänzt mit 5 % fötalem Kälberserum (Sigma, Deisenhofen, Deutschland) in einer befeuchteten 5 % CO2-Atmosphäre kultiviert. Für Transfektionen wurden die Zellen in 24- Loch-Platten gegeben und mit 2 μg Plasmid DNA nach der Calciumphos- phat-Copräzipitationsmethode wie von Roussel et al. (Mol. Cell. Biol. 4 (1984), 1999-2009) beschrieben transfiziert. Hierfür wurden 25 μ\ DNA Lösung mit 25 μ\ 2 x HBS-Puffer pH 6,9 (274 mM NaCI, 10 mM KCI, 40 mM Hepes, 1 ,4 mM Na2HPO4) bei 4 °C in einer 96-Loch-Platte mit einem 1 2-Kanal-Pipettierautomaten (Eppendorf, Hamburg, Deutschland) vermischt. Nach Zugabe von 20 μ\ einer 0,25 M CaCI2 Lösung (4 °C) und Mischen wurden 38 μ\ nach Inkubation für 25 min bei Raumtemperatur auf die Zellen gegeben.Human HEK 293T cells were supplemented in DMEM with 5% fetal calf serum (Sigma, Deisenhofen, Germany) in a humidified 5% CO 2 atmosphere. For transfections, the cells were placed in 24-well plates and treated with 2 μg plasmid DNA using the calcium phosphate coprecipitation method as described by Roussel et al. (Mol. Cell. Biol. 4 (1984), 1999-2009). For this, 25 μ \ DNA solution with 25 μ \ 2 x HBS buffer pH 6.9 (274 mM NaCl, 10 mM KCI, 40 mM Hepes, 1, 4 mM Na 2 HPO 4 ) at 4 ° C in a 96- Perforated plate mixed with a 1 2-channel pipetting machine (Eppendorf, Hamburg, Germany). After adding 20 μl of a 0.25 M CaCl 2 solution (4 ° C.) and mixing, 38 μl were added to the cells after incubation for 25 min at room temperature.
2.2 Erzeugung einer normalisierten Bibliothek und cDNA Screeninα Die Normalisierung und Konstruktion einer Nieren cDNA Bibliothek wurde wie von Grimm und Leder (J. Exp. Meth. 185 (1997), 1 137-1 142) und Sasaki et al. (Nucleic Acids Res. 22 (1994), 987-992) beschrieben durchgeführt.2.2 Generation of a normalized library and cDNA Screeninα The normalization and construction of a kidney cDNA library was carried out as described by Grimm and Leder (J. Exp. Meth. 185 (1997), 1 137-1 142) and Sasaki et al. (Nucleic Acids Res. 22 (1994), 987-992).
mRNA aus der Niere von 10 Wochen alten CD1 Mäusen wurde durch Assoziation abundanter mRNA Spezies mit kovalent an Latexbeads gekoppelten Antisense-cDNA-Molekülen und anschließende Abtrennung durch Zentrifugation normalisiert. Nach zwei Hybridisierungsrunden wurden 200 ng (von ursprünglich 2 μg) mRNA erhalten und zur Herstellung einer cDNA Bibliothek unter Verwendung eines cDNA Synthesekits (Gibco BRL, Gaithersburg, MD) verwendet. Nach igation eines BstXI Adaptors (Invitrogen, San Diego, CA) und einer Spaltung mit Notl wurden die cDNA Moleküle in einen modifizierten pcDNA3-Vektσr (Invitrogen) unter Kontrolle des Cytomegalovirus (CMV) Promotors inseriert, in dem das Neomycinresistenzgen deletiert worden war. Die DNA wurde durch Elektroporation in E.coli SURE-Zellen (Stratagene, Corp. La Jolla, CA) eingeführt, die anschließend sofort eingefroren wurden.Kidney mRNA from 10 week old CD1 mice was normalized by association of abundant mRNA species with antisense cDNA molecules covalently coupled to latex beads and subsequent separation by centrifugation. After two rounds of hybridization, 200 ng (from originally 2 μg) of mRNA were obtained and used to prepare a cDNA library using a cDNA synthesis kit (Gibco BRL, Gaithersburg, MD) used. After ligating a BstXI adapter (Invitrogen, San Diego, CA) and cleaving with Notl, the cDNA molecules were inserted into a modified pcDNA3 vector (Invitrogen) under the control of the cytomegalovirus (CMV) promoter in which the neomycin resistance gene had been deleted. The DNA was introduced by electroporation into E. coli SURE cells (Stratagene, Corp. La Jolla, CA), which were then immediately frozen.
Durch Ausplattieren von Aliquots des Transformationsansatzes auf Agar wurde gefunden, dass die Bibliothek etwa 2,5x105 Klone enthielt. Aliquots, die statistisch Einzelklone enthielten, wurden in Löchern von 96-Loch- Blöcken (Qiagen, Hilden, Deutschland) in 900 μ\ LB-Medium inokuliert und für 30 h unter Schütteln bei 300 Upm kultiviert. Die verbleibende DNA wurde zur Transformation von Bakterien für eine Plasmidisolierung im großen Maßstab und zur Sequenzierung der insertierten DNA verwendet. Anhand der DNA-Sequenz wurde mit Hilfe des Computerprogramms "Blast" ein Sequenzvergleich mit kommerziellen Sequenzdatenbanken durchgeführtBy plating aliquots of the transformation mixture on agar, it was found that the library contained approximately 2.5x10 5 clones. Aliquots containing statistically single clones were inoculated in holes of 96-hole blocks (Qiagen, Hilden, Germany) in 900 μ \ LB medium and cultured for 30 hours with shaking at 300 rpm. The remaining DNA was used to transform bacteria for large-scale plasmid isolation and to sequence the inserted DNA. On the basis of the DNA sequence, a sequence comparison with commercial sequence databases was carried out with the aid of the "Blast" computer program
2.3 Bestimmung der Apoptose-induzierenden Nukleinsäuren Die Apoptose-induzierende Aktivität der transfizierten Nukleinsäuren erfolgte durch mikroskopische Bestimmung des Zellphänotyps. Bei apoptotischen Zellen nimmt die optische Dichte der Zellen zu, da sich das Cytoplasma-Kernvolumen-Verhältnis verringert und durch den Abbau des Cytoskeletts bilden sich Blasen in der Cytoplasmamembran.2.3 Determination of the Apoptosis-Inducing Nucleic Acids The apoptosis-inducing activity of the transfected nucleic acids was carried out by microscopic determination of the cell phenotype. In the case of apoptotic cells, the optical density of the cells increases because the cytoplasmic nucleus volume ratio decreases and as a result of the breakdown of the cytoskeleton, bubbles form in the cytoplasmic membrane.
2.4 Plasmidisolierung2.4 Plasmid isolation
96-Loch-Blöcke mit Bakterien wurden für 5 min bei 3000 g (Sigma Zentrifugen, Osterode am Harz, Deutschland) zentrifugiert. Der Überstand wurde dekantiert und die Blöcke wurden für 2 bis 3 min umgedreht. Dann wurden 1 70 μ\ Puffer P1 (50 mM Tris-HCI/1 OmM EDTA pH 8,0) zugegeben und die Bakterienpellets wurden durch vollständige Vortexbehandlung für 10 bis 20 min resuspendiert. Nach Zugabe von 1 70 μ\ Puffer P2 (200 mM NaOH, 1 % SDS) wurde der Block mit Folie abgedichtet, durch Invertieren gemischt und für 5 min bei Raumtemperatur inkubiert. Die Lyse wurde durch Zugabe von 170 μ\ von 4°C kaltem Puffer P3 (3 M Kaliumacetat pH 5,5) beendet. Dann wurden 10 μ\ RNaseA Lösung ( 1 ,7 mg/ml) zugegeben, für 5 min bei Raumtemperatur und dann bei -20°C inkubiert und erneut für 10 min bei 6000 Upm zentrifugiert. Der Überstand wurde in neue Blöcke dekantiert und 100 μ\ Puffer P4 (2,5% SDS in Isopropanol) wurden zugegeben. Der Block wurde einer Vortexbehandlung für 5 min unterzogen und zuerst für 1 5 min bei 4°C und dann für 15 min bei -20°C inkubiert.96-hole blocks with bacteria were centrifuged for 5 min at 3000 g (Sigma centrifuges, Osterode am Harz, Germany). The supernatant was decanted and the blocks were inverted for 2-3 minutes. Then 1 70 μ \ buffer P1 (50 mM Tris-HCl / 1 OmM EDTA pH 8.0) was added and the bacterial pellets were resuspended by complete vortex treatment for 10 to 20 min. After adding 1 70 μ \ buffer P2 (200 mM NaOH, 1% SDS), the block was sealed with film, mixed by inverting and incubated for 5 min at room temperature. The lysis was terminated by adding 170 μl of 4 ° C. cold buffer P3 (3 M potassium acetate pH 5.5). Then 10 μ RNaseA solution (1.7 mg / ml) were added, incubated for 5 min at room temperature and then at -20 ° C. and centrifuged again for 10 min at 6000 rpm. The supernatant was decanted into new blocks and 100 μl buffer P4 (2.5% SDS in isopropanol) was added. The block was vortexed for 5 min and incubated first for 15 min at 4 ° C and then for 15 min at -20 ° C.
Der Überstand nach Zentrifugation für 10 min bei 6000 Upm wurde in 96- Loch- Polyoxymethylen-M ikrotiterblöcke gegeben . 1 50 μl Siliciumoxidsuspension wurden zugegeben und für 20 min bei Raumtemperatur inkubiert. Die Platten wurden für 5 min bei 6000 Upm zentrifugiert. Der Überstand wurde sorgfältig dekantiert und 400 μl Aceton (-20°C) wurden zugegeben. Die Platten wurden erneut einer Vortexbehandlung (30 sec) unterzogen und für 3 min bei 6000 Upm zentrifugiert. Dieser Acetonwaschvorgang wurde einmal wiederholt. Die Platten wurden zuerst bei Raumtemperatur für 5 min und dann für 5 min in einer Vakuumkammer getrocknet. Die Pellets wurden in 75 μl Wasser (60 °C) resuspendiert und bei 6000 Upm und 4°C 10 min zentrifugiert. Der Überstand wurde in einer 96-Loch-Mikrotiterplatte bei -20 °C aufbewahrt.The supernatant after centrifugation for 10 min at 6000 rpm was placed in 96-hole polyoxymethylene microtiter blocks. 1 50 ul silica suspension was added and incubated for 20 min at room temperature. The plates were centrifuged for 5 minutes at 6000 rpm. The supernatant was carefully decanted and 400 ul acetone (-20 ° C) was added. The plates were again vortexed (30 sec) and centrifuged for 3 min at 6000 rpm. This acetone washing process was repeated once. The plates were first dried at room temperature for 5 minutes and then for 5 minutes in a vacuum chamber. The pellets were resuspended in 75 ul water (60 ° C) and centrifuged at 6000 rpm and 4 ° C for 10 min. The supernatant was stored in a 96-well microtiter plate at -20 ° C.
ErgebnisseResults
Klon 1 setzt sich aus folgenden Sequenzen zusammen: T7-, BGH-Sequenz, interner Primer links und interner Primer rechts (Seq. 1-4). Die Gesamtsequenz ist unter Contig (Seq. 5) angegeben. Klon 1 zeigt Homologie mit folgenden ESTs (expressed sequence tags): uc81 e12.y1 Sugano mouse kidney mkia Mus musculus cDNA clone (Genbank Acc. No AA986577.1 | AA986577) mit einer Identität von 99%; mq28c01.r1 Barstead MPLRB1 Mus musculus cDNA clone (Genbank Acc. No AA1 37597. 1 J AA1 37597) mit einer Identität von 100%; vc94d05.r1 Barstead MPLRB1 Mus musculus cDNA clone (Genbank Acc. No AA388338.1 | AA388338) mit einer Identität von 99%; vg82f08.r1 Barstead MPLRB1 Mus musculus cDNA clone (Genbank Acc. No AI098009.1 | AI098009) mit einer Identität von 99%; uc81 f01 .x1 Sugano mouse kidney mkia Mus musculus cDNA clone (Genbank Acc. No AA986094.1 | AA986094) mit einer Identität von 100%.Clone 1 is composed of the following sequences: T7, BGH sequence, internal primer on the left and internal primer on the right (Seq. 1-4). The overall sequence is given under Contig (Seq. 5). Clone 1 shows homology with the following ESTs (expressed sequence tags): uc81 e12.y1 Sugano mouse kidney mkia Mus musculus cDNA clone (Genbank Acc. No AA986577.1 | AA986577) with an identity of 99%; mq28c01.r1 Barstead MPLRB1 Mus musculus cDNA clone (Genbank Acc. No AA1 37597. 1 J AA1 37597) with an identity of 100%; vc94d05.r1 Barstead MPLRB1 Mus musculus cDNA clone (Genbank Acc. No AA388338.1 | AA388338) with an identity of 99%; vg82f08.r1 Barstead MPLRB1 Mus musculus cDNA clone (Genbank Acc. No AI098009.1 | AI098009) with an identity of 99%; uc81 f01 .x1 Sugano mouse kidney mkia Mus musculus cDNA clone (Genbank Acc. No AA986094.1 | AA986094) with an identity of 100%.
Klon 2 setzt sich aus T7- und BGH-Sequenz zusammen. Die Gesamtsequenz ist unter Contig angegeben. Klon 2 zeigt Homologie mit folgenden Datenbankeinträgen: Homo sapiens cDNA FLJ20625 fis, clone KAT04008 (DNA Data Bank of Japan (DDBJ) Acc. No AK000632.1 j AK000632) mit einer Identität von 91 %; mf71 g09.r1 Soares mouse embryo NbME1 3.5 14.5 Mus musculus cDNA clone (Genbank Acc. No W89554.1 J W89554) mit einer Identität von 99%; G0103H 1 2-3 Mouse E7.5 Embryonic Portion cDNA Library Mus musculus cDNA clone (Genbank Acc. No AW536394.1 j AW536394) mit einer Identität von 99%.Clone 2 is composed of the T7 and BGH sequences. The overall sequence is given under Contig. Clone 2 shows homology with the following database entries: Homo sapiens cDNA FLJ20625 fis, clone KAT04008 (DNA Data Bank of Japan (DDBJ) Acc. No AK000632.1 and AK000632) with an identity of 91%; mf71 g09.r1 Soares mouse embryo NbME1 3.5 14.5 Mus musculus cDNA clone (Genbank Acc. No W89554.1 J W89554) with an identity of 99%; G0103H 1 2-3 Mouse E7.5 Embryonic Portion cDNA Library Mus musculus cDNA clone (Genbank Acc. No AW536394.1 j AW536394) with an identity of 99%.
Klon 3 setzt sich aus T7- und BGH-Sequenz zusammen. Die Gesamtsequenz ist unter Contig angegeben. Klon 3 zeigt Homologie mit EMBL Acc. No X78936 | MMPHRPR: M. musculus mRNA for parathyroid hormone/parathyroid hormone related peptide receptor mit einer Identität von 94%.Clone 3 is composed of the T7 and BGH sequences. The overall sequence is given under Contig. Clone 3 shows homology with EMBL Acc. No X78936 | MMPHRPR: M. musculus mRNA for parathyroid hormone / parathyroid hormone related peptide receptor with an identity of 94%.
Klon 4 setzt sich aus T7- und BGH-Sequenz zusammen. Die Gesamtsequenz ist unter Contig angegeben. Klon 4 zeigt Homologie mit Mus musculus mRNA for calcium Channel gamma 5 subunit (CACNG5 gene) (EMBL Acc. No AJ272046.1 J MMU272046) mit einer Identität von 97%.Clone 4 is composed of the T7 and BGH sequences. The overall sequence is given under Contig. Clone 4 shows homology with Mus musculus mRNA for calcium Channel gamma 5 subunit (CACNG5 gene) (EMBL Acc. No AJ272046.1 J MMU272046) with an identity of 97%.
Klon 5 zeigt Homologie mit RefSeq Datenbank Acc. No NM_00821 8.1 : Mus musculus hemoglobin alpha, adult chain 1 (Hba-a1 ), mRNA mit einer Länge von 564bp und einer Identität von 97% sowie Genbank Acc. No L75940.1 1 MUSALGL: Mus musculus alpha-globin mRNA, complete cds mit einer Identität von 98%.Clone 5 shows homology with RefSeq database Acc. No NM_00821 8.1: Muscle hemoglobin alpha, adult chain 1 (Hba-a1), mRNA with one Length of 564bp and an identity of 97% as well as Genbank Acc. No L75940.1 1 MUSALGL: Mus muscle alpha-globin mRNA, complete cds with an identity of 98%.
Klon 6 setzt sich aus T7- bzw. R2 und BGH- bzw. 4SP6-Sequenz zusammen. Die Gesamtsequenz ist unter Contig angegeben. Klon 6 zeigt Homologie mit folgenden Datenbankeinträgen: Mus musculus domesticus mitochondrial carrier homolog 1 isoform a mRNA, complete cds; nuclear gene for mitochondrial (Genbank Acc. No AF176007.2 J AF176007) mit einer Identität von 99%; Homo sapiens CGI-64 protein mRNA, complete cds (Genbank Acc. No AF151822.1 | AF151822) mit einer Identität von 90%; Homo sapiens presenilin-associated protein mRNA, complete cds (Genbank Acc. No AF189289.1 | AF189289) mit einer Identität von 89%.Clone 6 is composed of T7 or R2 and BGH or 4SP6 sequence. The overall sequence is given under Contig. Clone 6 shows homology with the following database entries: Mus musculus domesticus mitochondrial carrier homolog 1 isoform a mRNA, complete cds; nuclear gene for mitochondrial (Genbank Acc. No AF176007.2 J AF176007) with an identity of 99%; Homo sapiens CGI-64 protein mRNA, complete cds (Genbank Acc. No AF151822.1 | AF151822) with an identity of 90%; Homo sapiens presenilin-associated protein mRNA, complete cds (Genbank Acc. No AF189289.1 | AF189289) with an identity of 89%.
Klon 7 zeigt Homologie zu Genbank Acc. No AF151893.1 | AF151893: Homo sapiens CGI-135 protein mRNA, complete cds mit einer Identität von 86%.Clone 7 shows homology to Genbank Acc. No AF151893.1 | AF151893: Homo sapiens CGI-135 protein mRNA, complete cds with an identity of 86%.
Klon 8 zeigt Homologie zu RefSeq Acc. No NM_012504.1 : Rattus norvegicus ATPase, Na + K -t- transporting, alpha 1 polypeptide (Atp1 a1 ), mRNA mit einer Identität von 94%.Clone 8 shows homology to RefSeq Acc. No NM_012504.1: Rattus norvegicus ATPase, Na + K -t-transporting, alpha 1 polypeptide (Atp1 a1), mRNA with an identity of 94%.
Klon 9 zeigt Homologie zu folgenden Datenbankeinträgen: Mus musculus solute carrier family 27 (fatty acid transporter), member 2 (Slc27a2), mRNA (RefSeq Acc. No NM_01 1978.1 ) mit einer Identität von 99%; Mus musculus fatty acid transport protein 2 mRNA, complete cds (Genbank Acc. No AF072757.1 J AF072757) mit einer Identität von 99%; Mus musculus mRNA for very-Iong-chain acyl-CoA synthetase (VLACS) (EMBL Acc. No AJ223958.1 J MMAJ3958) mit einer Identität von 99%.Clone 9 shows homology to the following database entries: Mus musculus solute carrier family 27 (fatty acid transporter), member 2 (Slc27a2), mRNA (RefSeq Acc. No NM_01 1978.1) with an identity of 99%; Mus muscle fatty acid transport protein 2 mRNA, complete cds (Genbank Acc. No AF072757.1 J AF072757) with an identity of 99%; Mus muscle mRNA for very-long-chain acyl-CoA synthetase (VLACS) (EMBL Acc. No AJ223958.1 J MMAJ3958) with an identity of 99%.
Klon 10 setzt sich aus T7- und BGH-Sequenz zusammen. Die Gesamtsequenz ist unter Contig angegeben. Klon 10 zeigt Homologie zu EMBL Acc. No AL080066.1 J HSM800567: Homo sapiens mRNA; cDNA DKFZp564J 142 (from clone DKFZp564J142) mit einer Identität von 89-95% sowie zu DDBJ Acc. No AK001993.1 | AK001993: Homo sapiens cDNA FLJ1 1 131 fis, clone PLACE 1006325, highly similar to Homo sapiens mRNA; cDNA DKFZp564J142 mit einer Identität von 89-95%.Clone 10 is composed of the T7 and BGH sequences. The overall sequence is given under Contig. Clone 10 shows homology EMBL Acc. No AL080066.1 J HSM800567: Homo sapiens mRNA; cDNA DKFZp564J 142 (from clone DKFZp564J142) with an identity of 89-95% as well as to DDBJ Acc. No AK001993.1 | AK001993: Homo sapiens cDNA FLJ1 1 131 fis, clone PLACE 1006325, highly similar to Homo sapiens mRNA; cDNA DKFZp564J142 with an identity of 89-95%.
Klon 1 1 zeigt Homologie, zu folgenden Datenbankeinträgen: RefSeq Acc. No NM_009984.1 : Mus musculus cathepsin L (Ctsl), mRNA mit einer Identität von 96%; EMBL Acc. No X06086.1 1 MMMEPR: Mouse mRNA for major excreted protein (MEP) mit einer Identität von 96%; Genbank Acc. No J02583.1 j MUSCPR: mit einer Identität von 96%.Clone 1 1 shows homology to the following database entries: RefSeq Acc. No NM_009984.1: Mus muscle cathepsin L (Ctsl), mRNA with an identity of 96%; EMBL Acc. No X06086.1 1 MMMEPR: Mouse mRNA for major excreted protein (MEP) with an identity of 96%; Genbank Acc. No J02583.1 j MUSCPR: with an identity of 96%.
Klon 12 setzt sich aus T7- und BGH-Sequenz zusammen. Die Gesamtsequenz ist unter Contig angegeben. Klon 12 zeigt Homologie zu folgenden Datenbankeinträgen: EMBL Acc. No AL137721.1 | HSM802233: Homo sapiens mRNA; cDNA DKFZp761 H221 mit einer Identität von 89%; Genbank Acc. No L34839.1 j HUMTUM: Homo sapiens over-expressed breast tumor protein mRNA mit einer Identität von 89%; Genbank Acc. No AF216754.1 J AF216754: Homo sapiens over-expressed breast tumor protein (OBTP) mRNA, complete cds mit einer Identität von 89%; Genbank Acc. No U16813 j HSU16813: Human Bak-3 pseudogene, complete cds mit einer Identität von 88%. Mit folgenden ESTs zeigt Klon 12 eine Homologie: DDBJ Acc. No C88895 | C88895: Mus musculus early blastocyst cDNA, clone 01 B00051 IK19 mit einer Identität von 97% und Genbank Acc. No AA268719 | AA268719: Soares mouse NML Mus musculus cDNA clone 747619 5' mit einer Identität von 97%:Clone 12 is composed of the T7 and BGH sequences. The overall sequence is given under Contig. Clone 12 shows homology to the following database entries: EMBL Acc. No AL137721.1 | HSM802233: Homo sapiens mRNA; cDNA DKFZp761 H221 with an identity of 89%; Genbank Acc. No L34839.1 j HUMTUM: Homo sapiens over-expressed breast tumor protein mRNA with an identity of 89%; Genbank Acc. No AF216754.1 J AF216754: Homo sapiens over-expressed breast tumor protein (OBTP) mRNA, complete cds with an identity of 89%; Genbank Acc. No U16813 j HSU16813: Human Bak-3 pseudogenic, complete cds with an identity of 88%. Clone 12 shows homology with the following ESTs: DDBJ Acc. No C88895 | C88895: Mus musculus early blastocyst cDNA, clone 01 B00051 IK19 with an identity of 97% and Genbank Acc. No AA268719 | AA268719: Soares mouse NML Mus musculus cDNA clone 747619 5 'with an identity of 97%:
Klon 13 setzt sich aus T7- und BGH-Sequenz zusammen. Die Gesamtsequenz ist unter Contig angegeben. Klon 13 zeigt Homologie zu Genbank Acc. No AF151807.1 JAF151807: Homo sapiens CGI-49 protein mRNA, complete cds mit einer Identität von 87-90%. Klon 14 zeigt eine Homologie zu Genbank Acc. No AF080469] AF080469: Mus musculus putative glycogen storage disease type 1b protein mRNA, complete cds mit einer Identität von 94%.Clone 13 is composed of the T7 and BGH sequences. The overall sequence is given under Contig. Clone 13 shows homology to Genbank Acc. No AF151807.1 JAF151807: Homo sapiens CGI-49 protein mRNA, complete cds with an identity of 87-90%. Clone 14 shows homology to Genbank Acc. No AF080469] AF080469: Mus muscle putative glycogen storage disease type 1b protein mRNA, complete cds with an identity of 94%.
Klon 15 zeigt eine Identität zu Genbank Acc. No U31241 |CGU31241 : Cricetulus griseus integral membrane protein CII-3 mRNA, nuclear gene encoding mitochondrial protein, complete cds mit einer Identität von 85% sowie zu Genbank Acc. No S74803JS74803: CII-3 succinate-ubiquinone oxidoreductase complex II membrane-intrinsic subunit [cattle, heart, mRNA mit einer Identität von 83%.Clone 15 shows an identity to Genbank Acc. No U31241 | CGU31241: Cricetulus griseus integral membrane protein CII-3 mRNA, nuclear gene encoding mitochondrial protein, complete cds with an identity of 85% and to Genbank Acc. No S74803JS74803: CII-3 succinate-ubiquinone oxidoreductase complex II membrane-intrinsic subunit [cattle, heart, mRNA with an identity of 83%.
Klon 16 setzt sich aus T7- bzw.4SP6- und BGH-Sequenz zusammen. Die Gesamtsequenz ist unter Contig angegeben. Klon 16 zeigt eine Homologie zu Genbank Acc. No AF116911.1 |AF116911 : Mus musculus thymic dendritic cell-derived factor 1 mRNA, complete cds mit einer Identität von 96% sowie zu RefSeq Acc. No NM_004872.1: Homo sapiens mouse tropomyosin homolog (HSPC001) mRNA mit einer Identität von 89-92%.Clone 16 is composed of T7 or 4SP6 and BGH sequences. The overall sequence is given under Contig. Clone 16 shows homology to Genbank Acc. No AF116911.1 | AF116911: Mus musculus thymic dendritic cell-derived factor 1 mRNA, complete cds with an identity of 96% and for RefSeq Acc. No NM_004872.1: Homo sapiens mouse tropomyosin homolog (HSPC001) mRNA with an identity of 89-92%.
Klon 17 zeigt eine Identität zu Genbank Acc. No AF056031 JAF056031: Rattus norvegicus kynurenine 3-hydroxylase mRNA, complete cds mit eine Identität von 90% sowie zu EMBL Acc. No Y1.3153JHSKYNU3MO: Homo sapiens mRNA for kynurenine 3-monooxygenase mit einer Identität von 82%.Clone 17 shows an identity to Genbank Acc. No AF056031 JAF056031: Rattus norvegicus kynurenine 3-hydroxylase mRNA, complete cds with an identity of 90% and EMBL Acc. No Y1.3153JHSKYNU3MO: Homo sapiens mRNA for kynurenine 3-monooxygenase with an identity of 82%.
Klon 18 setzt sich aus T7- und BGH-Sequenz zusammen. Die Gesamtsequenz ist unter Contig angegeben. Klon 18 zeigt Homologie zu Genbank Acc. No AW109849.11 AW109849: MT2475 mouse liver, dioxin treated Mus musculus cDNA clone MT24753' mit einer identität von 94 %; Genbank Acc. No AA277327.1 | AA277327: va81 e12.r1 Soares mouse NML Mus musculus cDNA clone IMAGE:7378065' similar to WP:C02F5.3 0.CE00039 GTP-BINDING PROTEIN mit einer Identität von 93%; Genbank Acc . No A I 646762.1 | A I 646762: u b65e01 . x 1 Soares_mammary_gland_NMLMG Mus musculus cDNA clone IMAGE:13826163' similarto WP:C41D11.5 CE08662 ENDONUCLEASE mit einer Identität von 94%.Clone 18 is composed of the T7 and BGH sequences. The overall sequence is given under Contig. Clone 18 shows homology to Genbank Acc. No AW109849.11 AW109849: MT2475 mouse liver, dioxin treated Mus musculus cDNA clone MT24753 'with an identity of 94%; Genbank Acc. No AA277327.1 | AA277327: va81 e12.r1 Soares mouse NML Mus musculus cDNA clone IMAGE: 7378065 'similar to WP: C02F5.3 0.CE00039 GTP-BINDING PROTEIN with an identity of 93%; Genbank Acc. No AI 646762.1 | AI 646762: u b65e01. x 1 Soares_mammary_gland_NMLMG Mus musculus cDNA clone IMAGE: 13826163 'similarto WP: C41D11.5 CE08662 ENDONUCLEASE with an identity of 94%.
Klon 19 zeigt eine Homologie zu DDBJ Acc. No AB005451 |AB005451: Mus musculus mRNA for RST, complete cds mit einer Identität von 92%.Clone 19 shows homology to DDBJ Acc. No AB005451 | AB005451: Mus musculus mRNA for RST, complete cds with an identity of 92%.
Klon 20 zeigt Homologie zu Genbank Acc. No AA109018| AA109018; mp37f03.r1 Barstead MPLRB1 Mus musculus cDNA clone 571421 5' similar to SW:ACY2_HUMAN P45381 ASPARTOACYLASE mit einer Identität von 96%.Clone 20 shows homology to Genbank Acc. No AA109018 | AA109018; mp37f03.r1 Barstead MPLRB1 Mus musculus cDNA clone 571421 5 'similar to SW: ACY2_HUMAN P45381 ASPARTOACYLASE with an identity of 96%.
Klon 21 zeigt Homologie zu folgenden Datenbankeinträgen: Genbank Acc. No M12673JRATGNPAS: RATGNPAS Rat guanine nucleotide-binding protein G-s, alpha subunit mRNA complete cds mit einer Identität von 87% sowie zu EMBL Acc. No Y00703|MMGTPAMU: Mouse uncoupled S49 cells mRNA for stimulatory GTP-binding protein alpha subunit mit einer Identität von 87%; Genbank Acc. No M17525JRATBPGTPD: RATBPGTPD Rat GTP-binding protein (G-alpha-8) mRNA, complete cds mit einer Identität von 87%; Genbank Acc. No AF116268 |AF116268: Mus musculus G-protein XLAS (Xlas) mRNA, altematively spliced, complete cds mit einer Identität von 94%.Clone 21 shows homology to the following database entries: Genbank Acc. No M12673JRATGNPAS: RATGNPAS Rat guanine nucleotide-binding protein G-s, alpha subunit mRNA complete cds with an identity of 87% and EMBL Acc. No Y00703 | MMGTPAMU: Mouse uncoupled S49 cells mRNA for stimulatory GTP-binding protein alpha subunit with an identity of 87%; Genbank Acc. No M17525JRATBPGTPD: RATBPGTPD Rat GTP-binding protein (G-alpha-8) mRNA, complete cds with an identity of 87%; Genbank Acc. No AF116268 | AF116268: Mus musculus G-protein XLAS (Xlas) mRNA, altematively spliced, complete cds with an identity of 94%.
Klon 22 zeigt Homologie zu folgenden Datenbankeinträgen: Genbank Acc. No AF061026|AF061026: Mus musculus leucine zipper-EF-hand containing transmembrane protein 1 (Letml ) mRNA, complete cds mit einer Identität von 93-95%.Clone 22 shows homology to the following database entries: Genbank Acc. No AF061026 | AF061026: Mus musculus leucine zipper-EF-hand containing transmembrane protein 1 (Letml) mRNA, complete cds with an identity of 93-95%.
Klon 23 zeigt Homologie zu folgenden Datenbankeinträgen: Genbank Acc. No AA086895JAA086895: mk19c02.r1 Soares mouse p3NMF19.5 Mus musculus cDNA clone 4933465' similar to WP:F17C11.8 CE05655 sowie zu Genbank Acc. No AA881548|AA881548: vx20b03.r1 Soares 2NbMT Mus musculus cDNA clone 1264973 5' mit einer Identität von 88% und zu Genbank Acc. No AA073042 J AA073042: mm78b04.r1 Stratagene mouse embryonic carcinomaRA (#937318) Mus musculus cDNA clone 53451 1 5' similar to WP:F17C1 1.8 mit einer Identität von 89%;Clone 23 shows homology to the following database entries: Genbank Acc. No AA086895JAA086895: mk19c02.r1 Soares mouse p3NMF19.5 Mus musculus cDNA clone 4933465 'similar to WP: F17C11.8 CE05655 and to Genbank Acc. No AA881548 | AA881548: vx20b03.r1 Soares 2NbMT Mus musculus cDNA clone 1264973 5 'with an identity of 88% and to Genbank Acc. No AA073042 J AA073042: mm78b04.r1 Stratagene mouse embryonic carcinomaRA (# 937318) Mus musculus cDNA clone 53451 1 5 'similar to WP: F17C1 1.8 with an identity of 89%;
Klon 24 zeigt Homologie zu Genbank Acc. No U22465 | MMU22465: Mus musculus Na/Pi-cotransporter (NaPi-7) mRNA, complete cds mit einer Identität von 93% sowie zu Genbank Acc. No L33878| MUSSPT: Mus musculus renal sodium phosphate (Na + /Pi) transporter mRNA complete cds mit einer Identität von 93 %.Clone 24 shows homology to Genbank Acc. No U22465 | MMU22465: Mus musculus Na / Pi-cotransporter (NaPi-7) mRNA, complete cds with an identity of 93% and to Genbank Acc. No L33878 | MUSSPT: Mus musculus renal sodium phosphate (Na + / Pi) transporter mRNA complete cds with an identity of 93%.
Klon 25 zeigt Homologie zu Genbank Acc. No AF049882: Rattus norvegicus metastasis suppressor homolog (KAU ) mRNA, complete cds sowie zu DDBJ Acc.No D14883| MUSC33R2IA: Mouse mRNA for C33/R2/IA4, complete cds mit einer Identität von 94%. Für Klon 25 wurde gezeigt, dass C33-induzierte Apoptose durch die Induktion von Sauerstoffradikalen vermittelt wird, die zur proapoptotischen Aktivierung von Mitochondrien führen. Diese Sauerstoff radikale sind nicht durch die mitochondriale Atmungskette erzeugt, da Zellen mit einem genetischen Defekt in der Atmungskette immer noch mit Apoptose auf C33 Expression reagieren.Clone 25 shows homology to Genbank Acc. No AF049882: Rattus norvegicus metastasis suppressor homolog (KAU) mRNA, complete cds and for DDBJ Acc.No D14883 | MUSC33R2IA: Mouse mRNA for C33 / R2 / IA4, complete cds with an identity of 94%. For clone 25, it was shown that C33-induced apoptosis is mediated by the induction of oxygen radicals, which lead to proapoptotic activation of mitochondria. These oxygen radicals are not generated by the mitochondrial respiratory chain, since cells with a genetic defect in the respiratory chain still respond to C33 expression with apoptosis.
Die proapoptotische Aktivität von C33 ist nicht abhängig von Substratoder Zeil-Zeil- Interaktion, da auch Suspensionszellen noch durch C33 apoptotisch werden. Auch die schnelle Kinetik der Apoptose-Induktion von C33 unterscheidet sich von der bisher behaupteten proapoptotischen Effekten von C33..Desweiteren wurde gezeigt, daß ein extrazellulärer Loop in C33, der anscheinend für die Substrat-Interaktion verantwortlich ist, für die Apoptoseinduktion nicht notwendig ist.The proapoptotic activity of C33 is not dependent on substrate or cell-cell interaction, since suspension cells also become apoptotic by C33. The rapid kinetics of the apoptosis induction of C33 also differs from the previously claimed proapoptotic effects of C33. Furthermore, it was shown that an extracellular loop in C33, which apparently is responsible for the substrate interaction, is not necessary for the induction of apoptosis.
Klon 26 zeigt Homologie zu Genbank Acc. No U50987| BTU50987: Bos taurus succinate-ubiquinone reductase membrane anchor subunit precursor QPs3 mRNA, complete cds mit einer Identität von 86% sowie zu DDBJ AB006202: Homo sapiens mRNA for cytochrome b small subunit of complex II, complete cds mit einer Identität von 82%.Clone 26 shows homology to Genbank Acc. No U50987 | BTU50987: Bos taurus succinate-ubiquinone reductase membrane anchor subunit precursor QPs3 mRNA, complete cds with an identity of 86% and DDBJ AB006202: Homo sapiens mRNA for cytochrome b small subunit of complex II, complete cds with an identity of 82%.
Klon 27 zeigt Homologie zu Genbank Acc. No M31775 | MUSCYTB558: Mouse cytochrome beta-558 mRNA, 3' end mit einer Identität von 88%.Clone 27 shows homology to Genbank Acc. No M31775 | MUSCYTB558: Mouse cytochrome beta-558 mRNA, 3 'end with an identity of 88%.
Klon 28 zeigt Homologie zu Genbank Acc. No U76253 J MMU76253: Mus musculus E25B protein mRNA, complete cds mit einer Identität von 88%.Clone 28 shows homology to Genbank Acc. No U76253 J MMU76253: Mus musculus E25B protein mRNA, complete cds with an identity of 88%.
Klon 29 zeigt Homologie zu EMBL Acc. No Z98200.81 HS1 1 1 B22: Human DNA sequence from clone RP1-1 1 1 B22 on chromosome 6q16-21 Contains a novel pseudogene, a pseudogene similar to ribosomal protein L3, ESTs, STSs, GSSs and CpG Islands, complete sequence [Homo sapiens] mit einer Identität von 85% sowie zu Genbank Acc. No AW489000.1 | AW489000: UI-M-BH3-asd-a-10-0-Ul.s1 NIH_BMAPJV1_S4 Mus musculus cDNA clone UI-M-BH3-asd-a-10-0-UI 3' mit einer Identität von 85% und zu Genbank Acc. No AW488401 .1 | AW488401 : UI-M-BH3-art-b-02-0-Ul.s1 NIH_BMAP_M_S4 Mus musculus cDNA clone UI-M-BH3-art-b-02-0-UI 3' mit einer Identität von 98%.Clone 29 shows homology to EMBL Acc. No Z98200.81 HS1 1 1 B22: Human DNA sequence from clone RP1-1 1 1 B22 on chromosome 6q16-21 Contains a novel pseudogene, a pseudogene similar to ribosomal protein L3, ESTs, STSs, GSSs and CpG Islands, complete sequence [ Homo sapiens] with an identity of 85% and Genbank Acc. No AW489000.1 | AW489000: UI-M-BH3-asd-a-10-0-Ul.s1 NIH_BMAPJV1_S4 Mus musculus cDNA clone UI-M-BH3-asd-a-10-0-UI 3 'with an identity of 85% and to Genbank Acc. No AW488401 .1 | AW488401: UI-M-BH3-art-b-02-0-Ul.s1 NIH_BMAP_M_S4 Mus musculus cDNA clone UI-M-BH3-art-b-02-0-UI 3 'with an identity of 98%.
Klon 30 zeigt Homologie zu folgenden Datenbankeinträgen: Genbank Acc. No AI1 15883|AI1 15883: ue96a12.y1 Sugano mouse embryo mewa Mus musculus cDNA clone 1498942 5', mRNA sequence [Mus musculus] mit einer Identität von 97%; Genbank Acc. No AA052396 J AA052396: mb67c07.r1 Soares mouse p3NMF19.5 Mus musculus cDNA clone 334476 5' mit einer Identität von 96%; Genbank Acc. No AA777720 JAA777720: zj06a08.s1 Soares fetal liver spieen 1 NFLS S1 Homo sapiens cDNA clone 449462 3' mit einer Identität von 91 %; Genabank Acc. No AA9601 16JAA9601 16: ub54e08.s1 Soares mouse mammary gland NMLMG Mus musculus cDNA clone 1381574 3' mit einer Identität von 91 %. Klon 31 zeigt Homologie zu folgenden Datenbankeinträgen: Genbank Acc. No AI31 5450 ] AI31 5450: uj46g05.y1 Sugano mouse liver mlia Mus musculus cDNA clone IMAGE-.1 923032 5', mRNA sequence [Mus musculus] mit einer Identität von 89%; Genbank Acc. No AI01 96781 AI01 9678: ua92d1 1 .r1 Soares mouse mammary gland NbMMG Mus musculus cDNA clone 1 364949 5' mit einer Identität von 89%;Clone 30 shows homology to the following database entries: Genbank Acc. No AI1 15883 | AI1 15883: ue96a12.y1 Sugano mouse embryo mewa Mus musculus cDNA clone 1498942 5 ', mRNA sequence [Mus musculus] with an identity of 97%; Genbank Acc. No AA052396 J AA052396: mb67c07.r1 Soares mouse p3NMF19.5 Mus musculus cDNA clone 334476 5 'with an identity of 96%; Genbank Acc. No AA777720 JAA777720: zj06a08.s1 Soares fetal liver spieen 1 NFLS S1 Homo sapiens cDNA clone 449462 3 'with an identity of 91%; Genabank Acc. No AA9601 16JAA9601 16: ub54e08.s1 Soares mouse mammary gland NMLMG Mus musculus cDNA clone 1381574 3 'with an identity of 91%. Clone 31 shows homology to the following database entries: Genbank Acc. No AI31 5450] AI31 5450: uj46g05.y1 Sugano mouse liver mlia Mus musculus cDNA clone IMAGE-.1 923032 5 ', mRNA sequence [Mus musculus] with an identity of 89%; Genbank Acc. No AI01 96781 AI01 9678: ua92d1 1 .r1 Soares mouse mammary gland NbMMG Mus musculus cDNA clone 1 364949 5 'with an identity of 89%;
Klon 32 zeigt Homologie zu Genbank Acc. No U071 59 | MMU071 59: Mus musculus medium-chain acyl-CoA dehydrogenase mRNA, complete cds mit einer Identität von 94%.Clone 32 shows homology to Genbank Acc. No U071 59 | MMU071 59: Muscle medium-chain acyl-CoA dehydrogenase mRNA, complete cds with an identity of 94%.
Klon 33 zeigt Homologie zu Genbank Acc. No M761 31 { MUSEF2: Mouse elongation factor 2 (ef-2) mRNA, 3' end mit einer Identität von 89)%.Clone 33 shows homology to Genbank Acc. No M761 31 {MUSEF2: Mouse elongation factor 2 (ef-2) mRNA, 3 'end with an identity of 89)%.
Klon 34 zeigt Homologie zu Genbank Acc. No AF072757 J AF072757: Mus musculus fatty acid transport protein 2 mRNA, complete cds mit einer Identität von 96%.Clone 34 shows homology to Genbank Acc. No AF072757 J AF072757: Mus musculus fatty acid transport protein 2 mRNA, complete cds with an identity of 96%.
Klon 35 zeigt Homologie zu EMBL Acc. No X76453 J RNHREV1 07: R.norvegicus (Sprague Dawley) H-rev107 mRNA mit einer Identität von 90% sowie EMBL Acc. No X92814J HSHREV107: H. sapiens mRNA for rat HREV107-Iike protein mit einer Identität von 85%.Clone 35 shows homology to EMBL Acc. No X76453 J RNHREV1 07: R.norvegicus (Sprague Dawley) H-rev107 mRNA with an identity of 90% and EMBL Acc. No X92814J HSHREV107: H. sapiens mRNA for rat HREV107-Iike protein with an identity of 85%.
Klon 36 zeigt Homologie zu Genbank Acc. No AW107362.1 | AW107362: um1 5a04.x1 Sugano mouse kidney mkia Mus musculus cDNA clone IMAGE: 21 92334 3' similar to SW:ACY2_H UMAN P45381 ASPARTOACYLASE mit einer Identität von 93%.Clone 36 shows homology to Genbank Acc. No AW107362.1 | AW107362: um1 5a04.x1 Sugano mouse kidney mkia Mus musculus cDNA clone IMAGE: 21 92334 3 'similar to SW: ACY2_H UMAN P45381 ASPARTOACYLASE with an identity of 93%.
Klon 37 zeigt Homologie zu folgenden Datenbankeinträgen: Genbank Acc. No AI31 5920 | AI31 5920: uj27f1 1 .y1 Sugano mouse kidney mkia Mus musculus cDNA clone IMAGE: 1 921 1 97 5' similar to TR:Q14521 Q14521Clone 37 shows homology to the following database entries: Genbank Acc. No AI31 5920 | AI31 5920: uj27f1 1 .y1 Sugano mouse kidney mkia Mus musculus cDNA clone IMAGE: 1 921 1 97 5 'similar to TR: Q14521 Q14521
GIANT LARVAE HOMOLOGUE; mRNA sequence [Mus musculus] mit einer Identität von 87%; Genbank Acc. No A1315072 J AI315072: uj'23e1 1.x1 Sugano mouse kidney mkia Mus musculus cDNA clone IMAGE: 1920812 3', mRNA sequence [Mus musculus] mit einer Identität von 94%; Genbank Acc. No AF1099051 MMHC213L3: Mus musculus major histocompatibility locus class III regions Hsc70t gene, partial cds; smRNP, G7A, NG23, MutS homolog, CLCP, NG24, NG25, and NG26 genes, complete cds; and unknown genes mit einer Identität von 82-96%; Genbank Acc. No AF1 10520 | MMHC425O18: Mus musculus major histocompatibility complex region NG27, NG28, RPS28, NADH oxidoreductase, NG29, KIFC1 , Fas-binding protein, BING 1 , tapasin, RalGDS-like, KE2, BING4, beta 1 ,3-galactosyl transferase, and RPS18 genes mit einer Identität von 82%.GIANT LARVAE HOMOLOGUE; mRNA sequence [Mus musculus] with one Identity of 87%; Genbank Acc. No A1315072 J AI315072: uj ' 23e1 1.x1 Sugano mouse kidney mkia Mus musculus cDNA clone IMAGE: 1920812 3', mRNA sequence [Mus musculus] with an identity of 94%; Genbank Acc. No AF1099051 MMHC213L3: Mus musculus major histocompatibility locus class III regions Hsc70t gene, partial cds; smRNP, G7A, NG23, MutS homolog, CLCP, NG24, NG25, and NG26 genes, complete cds; and unknown genes with an identity of 82-96%; Genbank Acc. No AF1 10520 | MMHC425O18: Mus muscle major histocompatibility complex region NG27, NG28, RPS28, NADH oxidoreductase, NG29, KIFC1, Fas-binding protein, BING 1, tapasin, RalGDS-like, KE2, BING4, beta 1, 3-galactosyl transferase, and RPS18 genes with an identity of 82%.
Klon 38 zeigt Homologie zu Genbank Acc. No S45663 J S45663: SC2 = synaptic glycoprotein [rats, brain, mRNA, 1 178 nt] mit einer Identität von 91 %.Clone 38 shows homology to Genbank Acc. No S45663 J S45663: SC2 = synaptic glycoprotein [rats, brain, mRNA, 1 178 nt] with an identity of 91%.
Klon 39 zeigt Homologie zu Genbank Acc. No AA763399 J AA763399: vw53h02.r1 Soares mouse mammary gland NMLMG Mus musculus cDNA clone 1247571 5' similar to WP:F32D8.4 CE05783 LACTATE DEHYDROGENASE mit einer Identität von 96%.Clone 39 shows homology to Genbank Acc. No AA763399 J AA763399: vw53h02.r1 Soares mouse mammary gland NMLMG Mus musculus cDNA clone 1247571 5 'similar to WP: F32D8.4 CE05783 LACTATE DEHYDROGENASE with an identity of 96%.
Klon 40 zeigt Homologie zu Genbank Acc. No U00677 | U00677: Mus musculus syntrophin-1 gene, complete cds mit einer Identität von 92%.Clone 40 shows homology to Genbank Acc. No U00677 | U00677: Mus musculus syntrophin-1 gene, complete cds with an identity of 92%.
Klon 41 zeigt Homologie zu Genbank Acc. No AC003043 J AC003043: Homo sapiens chromosome 17, clone HRPC1067M6, complete sequence [Homo sapiens] mit einer Identität von 87% sowie zu Genbank Acc. No AA109006 | AA109006: ml63d04.r1 Stratagene mouse testis (#937308) Mus musculus cDNA mit einer Identität von 95% und zu Genbank Acc. No AA985996 J AA985996: Sugano mouse liver mlia Mus musculus cDNA clone 1431282 3' similar to TR:O14589 014589 SIMILARITY TO Q09461 mit einer Identität von 93%. Klon 42 zeigt Homologie zu Genbank Acc. No U30838 | MMU30838: Mus musculus voltage dependent anion Channel 2 mRNA, nuclear gene encoding mitochondrial protein, complete cds mit einer Identität von 94% sowie zu Genabank Acc. No L08666 J HUMPORIN: Homo sapiens porin (por) mRNA, complete cds and truncated cds mit einer Identität von 94%:Clone 41 shows homology to Genbank Acc. No AC003043 J AC003043: Homo sapiens chromosome 17, clone HRPC1067M6, complete sequence [Homo sapiens] with an identity of 87% and to Genbank Acc. No AA109006 | AA109006: ml63d04.r1 Stratagene mouse testis (# 937308) Mus musculus cDNA with an identity of 95% and to Genbank Acc. No AA985996 J AA985996: Sugano mouse liver mlia Mus musculus cDNA clone 1431282 3 'similar to TR: O14589 014589 SIMILARITY TO Q09461 with an identity of 93%. Clone 42 shows homology to Genbank Acc. No U30838 | MMU30838: Mus musculus voltage dependent anion Channel 2 mRNA, nuclear gene encoding mitochondrial protein, complete cds with an identity of 94% as well as Genabank Acc. No L08666 J HUMPORIN: Homo sapiens porin (por) mRNA, complete cds and truncated cds with an identity of 94%:
Klon 43 zeigt Homologie zu folgenden Datenbankeinträgen: Genbank Acc. No M27071 J MUSDIS2M1 A: Mus musculus protein phosphatase type 1 (dis2m1 ) mRNA, complete cds mit einer Identität von 92%; Genbank Acc. No U53456 | MMU53456: Mus musculus protein phosphatase 1 cgamma (PP1 cgamma) mRNA, complete cds mit einer Identität von 98-100%; EMBL Acc. No X56438 J DMPP1A1 : D.melanogaster PP1 -alpha 96A gene for protein phosphatase 1 mit einer Identität von 80%; Genbank Acc. No M27067 J EMEBIMG: Aspergillus nidulans phosphoprotein phosphatase 1 mRNA, complete cds mit einer Identität von 80%; Genbank Acc. No M60215 J MZEZMPP1 : Z.mays protein phosphatase-1 (ZmPPD mRNA, complete cds mit einer Identität von 83%; Genbank Acc. No U00063 J CELF56C9: Caenorhabditis elegans cosmid F56C9 mit einer Identität von 81 -86%.Clone 43 shows homology to the following database entries: Genbank Acc. No M27071 J MUSDIS2M1 A: Mus muscle protein phosphatase type 1 (dis2m1) mRNA, complete cds with an identity of 92%; Genbank Acc. No U53456 | MMU53456: Muscle protein phosphatase 1 cgamma (PP1 cgamma) mRNA, complete cds with an identity of 98-100%; EMBL Acc. No X56438 J DMPP1A1: D.melanogaster PP1 -alpha 96A gene for protein phosphatase 1 with an identity of 80%; Genbank Acc. No M27067 J EMEBIMG: Aspergillus nidulans phosphoprotein phosphatase 1 mRNA, complete cds with an identity of 80%; Genbank Acc. No M60215 J MZEZMPP1: Z.mays protein phosphatase-1 (ZmPPD mRNA, complete cds with an identity of 83%; Genbank Acc. No U00063 J CELF56C9: Caenorhabditis elegans cosmid F56C9 with an identity of 81 -86%.
Klon 44 zeigt Homologie zu Genbank Acc. No U52842| MMU52842: Mus musculus kidney-specific transport protein mRNA, complete cds mit einer Identität von 99% sowie zu DDBJ Acc. No AB004559 ] AB004559: Rattus norvegicus mRNA for multispecific organic anion transporter, complete cds mit einer Identität von 93%.Clone 44 shows homology to Genbank Acc. No U52842 | MMU52842: Muscle kidney-specific transport protein mRNA, complete cds with an identity of 99% and on DDBJ Acc. No AB004559] AB004559: Rattus norvegicus mRNA for multispecific organic anion transporter, complete cds with an identity of 93%.
Klon 45 zeigt Homologie zu folgenden Datenbankeinträgen: Genbank Acc. No AF080252 J AF080252: Mus musculus serine/threonine protein kinase 51 PK(S) mRNA, complete cds mit einer Identität von 94%; Genbank Acc. No AF080253 |AF080253: Mus musculus serine/threonine protein kinase 51 PK(L) mRNA, complete cds mit einer Identität von 94%; DDBJ Acc. No AB000449 J AB000449: Homo sapiens mRNA for VRK1 , complete cds mit einer Identität von 83%.Clone 45 shows homology to the following database entries: Genbank Acc. No AF080252 J AF080252: Mus muscle serine / threonine protein kinase 51 PK (S) mRNA, complete cds with an identity of 94%; Genbank Acc. No AF080253 | AF080253: Mus muscle serine / threonine protein kinase 51 PK (L) mRNA, complete cds with an identity of 94%; DDBJ Acc. No AB000449 J AB000449: Homo sapiens mRNA for VRK1, complete cds with an identity of 83%.
Klon 46 zeigt Homologie zu folgenden Datenbankeinträgen: Genbank Acc. No J04806 j MUSOSP: Mus musculus osteopontin mRNA, complete cds mit einer Identität von 95%; EMBL Acc. No X1 3986 | MMPONTIN: Mouse mRNA for minopontin mit einer Identität von 96%; EMBL Acc. No X 1 61 51 | MMETA1 : Mouse mRNA for early T-lymphocyte activation 1 protein (ETa-1 ) mit einer Identität von 95%; Genbank Acc. No S781 77 1 S781 77: Eta-1 /Op (Eta-1 b) = early T-lymphocyte activator-1 [mice, C3H/HeJ, spieen, mRNA, 1087 nt] mit einer Identität von 98%.Clone 46 shows homology to the following database entries: Genbank Acc. No J04806 j MUSOSP: Muscle osteopontin mRNA, complete cds with an identity of 95%; EMBL Acc. No X1 3986 | MMPONTIN: Mouse mRNA for minopontin with an identity of 96%; EMBL Acc. No X 1 61 51 | MMETA1: Mouse mRNA for early T-lymphocyte activation 1 protein (ETa-1) with an identity of 95%; Genbank Acc. No S781 77 1 S781 77: Eta-1 / Op (Eta-1 b) = early T-lymphocyte activator-1 [mice, C3H / HeJ, spieen, mRNA, 1087 nt] with an identity of 98%.
Klon 47 zeigt Homologie zu DDBJ Acc. No AB006451 | AB006451 : Rattus norvegicus mRNA for Tim23, complete cds mit einer Identität von 94% sowie zu Genbank Acc. No AF0301 62 J AF0301 62: Homo sapiens inner mitochondrial membrane translocase Tim23 (TIM23) mRNA, nuclear gene encoding mitochondrial protein complete cds mit einer Identität von 89%.Clone 47 shows homology to DDBJ Acc. No AB006451 | AB006451: Rattus norvegicus mRNA for Tim23, complete cds with an identity of 94% and Genbank Acc. No AF0301 62 J AF0301 62: Homo sapiens inner mitochondrial membrane translocase Tim23 (TIM23) mRNA, nuclear gene encoding mitochondrial protein complete cds with an identity of 89%.
Klon 48 zeigt Homologie zu DDBJ Acc. No D38549 J HUMHA1025A: Human mRNA for KIAA0068 gene, partial cds mit einer Identität von 86% sowie zu Genbank Acc. No AF072697 J AF072697: Mus musculus SHYC (Shyc) mRNA, complete cds mit einer Identität von 96%.Clone 48 shows homology to DDBJ Acc. No D38549 J HUMHA1025A: Human mRNA for KIAA0068 genes, partial cds with an identity of 86% and to Genbank Acc. No AF072697 J AF072697: Mus muscle SHYC (Shyc) mRNA, complete cds with an identity of 96%.
Klon 49 zeigt Homologie zu Genbank Acc. No U07971 j RNU07971 : Rattus norvegicus Sprague-Dawley L-arginine:glycine amidinotransferase mRNA, partial cds mit einer Identität von 85%.Clone 49 shows homology to Genbank Acc. No U07971 j RNU07971: Rattus norvegicus Sprague-Dawley L-arginine: glycine amidinotransferase mRNA, partial cds with an identity of 85%.
Klon 50 zeigt Homologie zu DDBJ Acc. No AU035342 j AU035342: Sugano mouse brain mncb Mus musculus cDNA clone MNCb-0343, mRNA sequence [Mus musculus] mit einer Identität von 87% sowie zu GenbankClone 50 shows homology to DDBJ Acc. No AU035342 j AU035342: Sugano mouse brain mncb Mus musculus cDNA clone MNCb-0343, mRNA sequence [Mus musculus] with an identity of 87% and to Genbank
Acc. No AI286459 J AI286459: ui77d03.y1 Sugano mouse liver mlia Mus musculus cDNA clone IMAGE: 1888421 5' similar to WP:T06D8.9 CE02330, mRNA sequence [Mus musculus] mit einer Identität von 87%.Acc. No AI286459 J AI286459: ui77d03.y1 Sugano mouse liver mlia Mus musculus cDNA clone IMAGE: 1888421 5 'similar to WP: T06D8.9 CE02330, mRNA sequence [Mus musculus] with an identity of 87%.
Klon 51 zeigt Homologie zu Genbank Acc. No M22998| MUSGLUTRN: MUSGLUTRN Mouse facilitated glucose transport protein mRNA, complete cds mit einer Identität von 93% sowie zu Genabnk Acc. No S77924J S77924: Glut-1 = glucose transporter isoform 1 [mice, embryo, mRNA Partial, 321 nt] mit einer Identität von 93%.Clone 51 shows homology to Genbank Acc. No M22998 | MUSGLUTRN: MUSGLUTRN Mouse facilitated glucose transport protein mRNA, complete cds with an identity of 93% as well as Genabnk Acc. No S77924J S77924: Glut-1 = glucose transporter isoform 1 [mice, embryo, mRNA partial, 321 nt] with an identity of 93%.
Klon 52 zeigt Homologie zu EMBL Acc. No AJ010953 | HSA010953: Homo sapiens mRNA for putative Ca2 + -transporting ATPase, partial mit einer Identität von 79%.Clone 52 shows homology to EMBL Acc. No AJ010953 | HSA010953: Homo sapiens mRNA for putative Ca2 + -transporting ATPase, partial with an identity of 79%.
Klon 53 zeigt Homologie zu Acc. No U94593: Mus musculus uncoupling protein homolog (UCPH) mRNA.Clone 53 shows homology to Acc. No U94593: Mus muscle uncoupling protein homolog (UCPH) mRNA.
Klon 54 zeigt Homologie zu Acc. No M2731 5.1 : Rattus norvegicus mitochondrial cytochrome c oxidase subunits I, II and III, and ATPase subunit 6 genes, complete cds.Clone 54 shows homology to Acc. No M2731 5.1: Rattus norvegicus mitochondrial cytochrome c oxidase subunits I, II and III, and ATPase subunit 6 genes, complete cds.
Klon 55 setzt sich aus T7- und BGH-Sequenz zusammen. Die Gesamtsequenz ist unter Gesamtsequenz angegeben. Klon 12 zeigt Homologie zu folgenden Datenbankeinträgen: Acc. No AL080317.1 1 : Human DNA sequence from clone RP5-1 1 12D6 on chromosome 6q21-22.2. Contains the gene for a PUTATIVE novel protein similar to bacterial NARK (nitrite extrusion protein, nitrite facilitator), the 3' end of the REV3L gene for REV3 (yeast homologHike, catalytic subunit of DNA polymerase zeta (EC 2.7.7.7, POLZ), ESTs, STSs, GSSs and a putative CpG island, complete sequence. Klon 56 zeigt Homologie zu Acc. No AW106096.1 : um23a10.y1 Sugano mouse embryo mewa Mus musculus cDNA clone 1MAGE:2225370 5', mRNA sequence.Clone 55 is composed of the T7 and BGH sequences. The total sequence is given under total sequence. Clone 12 shows homology to the following database entries: Acc. No AL080317.1 1: Human DNA sequence from clone RP5-1 1 12D6 on chromosome 6q21-22.2. Contains the gene for a PUTATIVE novel protein similar to bacterial NARK (nitrite extrusion protein, nitrite facilitator), the 3 'end of the REV3L gene for REV3 (yeast homologHike, catalytic subunit of DNA polymerase zeta (EC 2.7.7.7, POLZ), ESTs, STSs, GSSs and a putative CpG island, complete sequence. Clone 56 shows homology to Acc. No AW106096.1: um23a10.y1 Sugano mouse embryo mewa Mus musculus cDNA clone 1MAGE: 2225370 5 ', mRNA sequence.
Klon 57 zeigt Homologie zu Acc. No NM_000210.1 Homo sapiens integrin, alpha 6 (ITGA6) mRNA.Clone 57 shows homology to Acc. No NM_000210.1 Homo sapiens integrin, alpha 6 (ITGA6) mRNA.
Klon 58 zeigt Homologie zu Acc. No AW106096. 1 : um23a 10.y1 Sugano mouse embryo mewa Mus musculus cDNA clone IMAGE:2225370 5', mRNA sequence.Clone 58 shows homology to Acc. No AW106096. 1: um23a 10.y1 Sugano mouse embryo mewa Mus musculus cDNA clone IMAGE: 2225370 5 ', mRNA sequence.
Klon 59 zeigt Homologie zu Acc. No NM_007748.1 : Mus musculus cytochrome c oxidase, subunit VI a, polypeptide 1 (Cox6a1 ), mRNA.Clone 59 shows homology to Acc. No NM_007748.1: Mus muscle cytochrome c oxidase, subunit VI a, polypeptide 1 (Cox6a1), mRNA.
Klon 60 zeigt Homologie zu Acc. No AC005403.1 : Mus musculus clone UWGC:ma53a068 from 14D1 -D2 (T-Cell Receptor Alpha Locus), complete sequence.Clone 60 shows homology to Acc. No AC005403.1: Mus musclulus clone UWGC: ma53a068 from 14D1 -D2 (T-Cell Receptor Alpha Locus), complete sequence.
Klon 61 zeigt Homologie zu Acc. No L07095.1 : Mus domesticus strain NZB/B1 NJ mitochondrion genome, complete sequence.Clone 61 shows homology to Acc. No L07095.1: Mus domesticus strain NZB / B1 NJ mitochondrion genome, complete sequence.
Klon 62 zeigt Homologie zu Acc. No NM_0091 55.1 : Mus musculus selenoprotein P, plasma, 1 (SeppD, mRNA complete cds.Clone 62 shows homology to Acc. No NM_0091 55.1: Mus muscle selenoprotein P, plasma, 1 (SeppD, mRNA complete cds.
Klon 63 zeigt Homologie zu Acc. No J03297.1 : Mouse ERp99 mRNA encoding an endoplasmic reticulum transmembrane protein.Clone 63 shows homology to Acc. No J03297.1: Mouse ERp99 mRNA encoding an endoplasmic reticulum transmembrane protein.
Klon 64 zeigt Homologie zu Acc. No AF047431 .1 : Homo sapiens AAPT1 -Iike protein mRNA, partial cds.Clone 64 shows homology to Acc. No AF047431 .1: Homo sapiens AAPT1-Iike protein mRNA, partial cds.
Klon 65 zeigt Homologie zu EMBL Acc. No U79287 Human clone 23867 mRNA sequence 1 396 bp. Klon 66 zeigt Homologie zu Acc. No C88489, AA073437, AI048028 und zu ESTs mit der DDBJ Acc. No C88489; Genbank Acc. No AA073437; AI048028.Clone 65 shows homology to EMBL Acc. No U79287 Human clone 23867 mRNA sequence 1 396 bp. Clone 66 shows homology to Acc. No C88489, AA073437, AI048028 and to ESTs with the DDBJ Acc. No C88489; Genbank Acc. No AA073437; AI048028.
Klon 67 zeigt Homologie zu Acc. No AK001441 : Homo sapiens cDNA FLJ 10579 fis, clone NT2RP2003446 2251 bp mRNA und zu ESTs mit der Acc. No AI663355 und AU080732.Clone 67 shows homology to Acc. No AK001441: Homo sapiens cDNA FLJ 10579 fis, clone NT2RP2003446 2251 bp mRNA and to ESTs with the Acc. No AI663355 and AU080732.
Klon 68 zeigt Homologie zu Acc. No AF005038: Homo sapiens Secretory Carrier Membrane Protein (SCAMP2) mRNAClone 68 shows homology to Acc. No AF005038: Homo sapiens Secretory Carrier Membrane Protein (SCAMP2) mRNA
Klon 69 zeigt Homologie zu Acc. No U44731 : Mus musculus putative purine nucleotide binding protein mRNA.Clone 69 shows homology to Acc. No U44731: Putative purine nucleotide binding protein mRNA muscle.
Klon 70 zeigt Homologie zu Acc. No U 1 681 8: Mus musculus UDP glucuronosyltransferase (UGT1 -06) mRNAClone 70 shows homology to Acc. No U 1 681 8: Muscle UDP glucuronosyltransferase (UGT1 -06) mRNA
Klon 71 zeigt Homologie zu Acc. No NM_004256: Homo sapiens organic cationic transporter-like 3 (ORCTL3) mRNA.Clone 71 shows homology to Acc. No NM_004256: Homo sapiens organic cationic transporter-like 3 (ORCTL3) mRNA.
Klon 72 zeigt Homologie zu Acc. No AK000559: Homo sapiens cDNA FLJ20552 fis, clone KAT1 1 732.Clone 72 shows homology to Acc. No AK000559: Homo sapiens cDNA FLJ20552 fis, clone KAT1 1 732.
Klon 73 zeigt Homologie zu Acc. No X89968: Rattus norvegicus mRNA for alpha-soluble NSF attachment protein.Clone 73 shows homology to Acc. No X89968: Rattus norvegicus mRNA for alpha-soluble NSF attachment protein.
Klon 74 zeigt Homologie zu Acc. No D851 37: Mouse mRNA for PPI gamma (protein phosphatasel gamma).Clone 74 shows homology to Acc. No D851 37: Mouse mRNA for PPI gamma (protein phosphatasel gamma).
Klon 75 zeigt Homologie zu Acc. No NM_001089: Homo sapiens ATP-binding cassette, sub-family A (ABC1 ), member 3 (ABCA3) mRNA. Klon 76 zeigt Homologie zu Maus-ESTs mit der Acc. No AI315969, AI930239, A1666299 und AV141 103.Clone 75 shows homology to Acc. No NM_001089: Homo sapiens ATP-binding cassette, sub-family A (ABC1), member 3 (ABCA3) mRNA. Clone 76 shows homology to mouse ESTs with the Acc. No AI315969, AI930239, A1666299 and AV141 103.
Klon 77 zeigt Homologie zu Acc. No AB025405: Mus musculus mRNA for sid2895p (Mouse microsomal signal peptidase)Clone 77 shows homology to Acc. No AB025405: Mus musculus mRNA for sid2895p (Mouse microsomal signal peptidase)
Klon 78 zeigt Homologie zu Acc. No AK000427. Der Klon ist partiell homolog zu: Homo sapiens cDNA FLJ20420 fis, clone KAT02462.Clone 78 shows homology to Acc. No AK000427. The clone is partially homologous to: Homo sapiens cDNA FLJ20420 fis, clone KAT02462.
Klon 79 zeigt Homologie zu Acc. No NM_009127: Mus musculus stearoyl-Coenzyme A desaturase 1 (Scd1 )Clone 79 shows homology to Acc. No NM_009127: Mus musculus stearoyl-Coenzyme A desaturase 1 (Scd1)
Klon 80 zeigt Homologie zu Acc. No U21049: Human DD96 mRNA (a gene selectively upregulated in human carcinomas).Clone 80 shows homology to Acc. No U21049: Human DD96 mRNA (a gene selectively upregulated in human carcinomas).
Klon 81 zeigt Homologie zu Acc. No AF070626 bzw. AB020980: Homo sapiens clone 24483 unknown mRNA (AF070626), bzw. Homo sapiens mRNA for putative membrane protein (AB020980).Clone 81 shows homology to Acc. No AF070626 or AB020980: Homo sapiens clone 24483 unknown mRNA (AF070626), or Homo sapiens mRNA for putative membrane protein (AB020980).
Klon 82 zeigt partielle Homologie zu Acc. No U92989: Homo sapiens clone DT1 P1 E1 1 mRNA, CAG repeat region.Clone 82 shows partial homology to Acc. No U92989: Homo sapiens clone DT1 P1 E1 1 mRNA, CAG repeat region.
Klon 83 zeigt Homologie zu Acc. No AF035208: Mus musculus putative v-SNARE Vtil b mRNA (soluble NSF attachment protein receptor).Clone 83 shows homology to Acc. No AF035208: Mus muscle putative v-SNARE Vtil b mRNA (soluble NSF attachment protein receptor).
Klon 84 zeigt Homologie zu Acc. No NM_012504: Rattus norvegicus ATPase, Na + K + transporting, alpha 1 polypeptide (Atp1 a1 ), mRNA.Clone 84 shows homology to Acc. No NM_012504: Rattus norvegicus ATPase, Na + K + transporting, alpha 1 polypeptide (Atp1 a1), mRNA.
Klon 85 zeigt Homologie zu Acc No NM_007749: Mus musculus cytochrome c oxidase subunit Vllc (Cox7c), mRNA. Klon 86 zeigt Homologie zu Acc No AF079565: Mus musculus ubiquitin-specific protease UBP41 (Ubp41 ) mRNA.Clone 85 shows homology to Acc No NM_007749: Mus musculus cytochrome c oxidase subunit Vllc (Cox7c), mRNA. Clone 86 shows homology to Acc No AF079565: Mus ubiquitin-specific protease UBP41 (Ubp41) mRNA.
Klon 87 zeigt Homologie zu Acc No AJ006341 : Mus musculus mRNA for peroxisomal integral membrane protein PMP34.Clone 87 shows homology to Acc No AJ006341: Mus musculus mRNA for peroxisomal integral membrane protein PMP34.
Klon 88 zeigt Homologie zu Acc No AF223950: Mus musculus TIM22 preprotein translocase (Tim22) mRNA, complete cds; nuclear gene for mitochondrial product.Clone 88 shows homology to Acc No AF223950: Mus musculus TIM22 preprotein translocase (Tim22) mRNA, complete cds; nuclear gene for mitochondrial product.
Klon 89 zeigt Homologie zu Acc No NM_01 1479: Mus musculus serine palmitoyltransferase, long chain base subunit 2 (Sptlc2), mRNA.Clone 89 shows homology to Acc No NM_01 1479: Mus muscle serine palmitoyltransferase, long chain base subunit 2 (Sptlc2), mRNA.
Klon 90 zeigt Homologie zu Acc No M23984: Rat mitochondrial proton/phosphate symporter mRNA, complete cds sowie zu Acc. No X60036: H. sapiens mRNA for mitochondrial phosphate carrier protein.Clone 90 shows homology to Acc No M23984: Rat mitochondrial proton / phosphate symporter mRNA, complete cds and Acc. No X60036: H. sapiens mRNA for mitochondrial phosphate carrier protein.
Klon 91 zeigt Homologie zu Acc No AF1 13127: Homo sapiens S1 R protein (S1 R) mRNA, complete cds (humanes Cowpoxvirus-Homolog).Clone 91 shows homology to Acc No AF1 13127: Homo sapiens S1 R protein (S1 R) mRNA, complete cds (human cowpox virus homolog).
Klon 92 setzt sich aus T7- und BGH-Sequenz zusammen. Die Gesamtsequenz ist als Zusammenführung mehrer EST-Sequenzen („EST-Assembly") in Contig gezeigt. Klon 92 zeigt eine Homologie zu UniGene Mm.86545.Clone 92 is composed of the T7 and BGH sequences. The entire sequence is shown as a merging of several EST sequences (“EST assembly”) in Contig. Clone 92 shows homology to UniGene Mm.86545.
Klon 93 zeigt Homologie zu humaner genomischer DNA (PAC 69E1 1 on chromosome 1 q23-24).Clone 93 shows homology to human genomic DNA (PAC 69E1 1 on chromosome 1 q23-24).
Klon 94 zeigt Homologie zu Acc No NM_01 1512: Mus musculus surfeit gene 4 (Surf4), mRNA. Klon 95 setzt sich aus T7- und BGH-Sequenz zusammen. Die Gesamtsequenz ist als Zusammenführung mehrer EST-Sequenzen („EST-Assembly") unter Gesamt cDNA EST-CIuster gezeigt. Klon 95 zeigt Homologie zu UniGene: Mm.27841 (Maus) sowie Hs.100132 (Mensch).Clone 94 shows homology to Acc No NM_01 1512: Mus musculus surfeit gene 4 (Surf4), mRNA. Clone 95 is composed of the T7 and BGH sequences. The entire sequence is shown as a combination of several EST sequences (“EST assembly”) under total cDNA EST clusters. Clone 95 shows homology to UniGene: Mm.27841 (mouse) and Hs.100132 (human).
Klon 96 zeigt Homologie zu UniGene Hs.133494, Mm.31778 und Rn.1 1778.Clone 96 shows homology to UniGene Hs.133494, Mm.31778 and Rn.1 1778.
Klon 97 zeigt Homologie zu Acc No AF192558: DEFINITION Mus musculus domesticus mitochondrial carrier homolog 1 isoform b (Mtchl ) mRNA, complete cds; nuclear gene for mitochondrial product sowie zu Acc. No AF192558 / AF192559: Homo sapiens mitochondrial carrier homolog 1 isoform b (MTCH1 ) mRNA, partial cds; nuclear gene for mitochondrial product.Clone 97 shows homology to Acc No AF192558: DEFINITION Mus musculus domesticus mitochondrial carrier homolog 1 isoform b (Mtchl) mRNA, complete cds; nuclear gene for mitochondrial product and Acc. No AF192558 / AF192559: Homo sapiens mitochondrial carrier homolog 1 isoform b (MTCH1) mRNA, partial cds; nuclear gene for mitochondrial product.
Klon 98 zeigt Homologie zu Acc No NM_016783: Mus musculus progesterone receptor membrane component (Pgrmc-pending), mRNA.Clone 98 shows homology to Acc No NM_016783: Mus musculus progesterone receptor membrane component (Pgrmc-pending), mRNA.
Klon 99 zeigt Homologie zu Acc No NM_003002: Homo sapiens succinate dehydrogenase complex, subunit D, integral membrane protein (SDHD) mRNA.Clone 99 shows homology to Acc No NM_003002: Homo sapiens succinate dehydrogenase complex, subunit D, integral membrane protein (SDHD) mRNA.
Klon 100 zeigt Homologie zu Acc No U95822: Human putative transmembrane GTPase mRNA, partial cds.Clone 100 shows homology to Acc No U95822: Human putative transmembrane GTPase mRNA, partial cds.
Klon 101 zeigt Homologie zu Acc No X99963: M. musculus rhoB gene.Clone 101 shows homology to Acc No X99963: M. musculus rhoB gene.
Klon 102 zeigt Homologie zu Acc No AF161 525: Homo sapiens HSPC177 mRNA, complete cds. Das Gen enthält eine BH3-Domäne, die in vielen Bcl-2-ähnlichen Genen vorkommt. Gene mit solchen "BH3-only"-Domänen sind proapoptotisch und haben die evolutiv konservierte Funktion, Apoptose zu induzieren. Die Versuche zeigten, daß CGl-135 mit Bcl-2-Genfamilienmitgliedern interagieren kann. Für die effiziente Apoptose-Induktion durch CGl-135 ist die BH3-Domäne notwendig.Clone 102 shows homology to Acc No AF161 525: Homo sapiens HSPC177 mRNA, complete cds. The gene contains a BH3 domain that is found in many Bcl-2-like genes. Genes with such "BH3-only" domains are pro-apoptotic and have the evolutionarily conserved function of inducing apoptosis. The experiments showed that CGl-135 with Bcl-2 gene family members can interact. The BH3 domain is necessary for efficient apoptosis induction by CGl-135.
Klon 103 zeigt keine Homologie zu bekannten Sequenzen.Clone 103 shows no homology to known sequences.
Klon 104 zeigt Homologie zu Unigene Acc No AW682500.30119 Mm.30119jMm.30119: Mus musculus cDNA mit einer Identität von 91%.Clone 104 shows homology to Unigene Acc No AW682500.30119 Mm.30119jMm.30119: Mus musculus cDNA with an identity of 91%.
Klon 105 zeigt Homologie zu EMBL Acc No X98475.1 jMMVASP: M. musculus VASP gene mit einer Identität von 90%.Clone 105 shows homology to EMBL Acc No X98475.1 jMMVASP: M. musculus VASP gene with an identity of 90%.
Klon 106 zeigt Homologie zu EMBL Acc No X03369.1 |RNTUBB15: Rat mRNA for beta-tubulin T beta15 mit einer Identität von 90%.Clone 106 shows homology to EMBL Acc No X03369.1 | RNTUBB15: Rat mRNA for beta-tubulin T beta15 with an identity of 90%.
Klon 107 zeigt Homologie zu Genbank Acc No BE226644.1 JBE226644: ia23h11.y1 Mouse E10 5 12 5 Pancreas cDNA Library Mus musculus cDNA 5' similar to SW:NUML_BOVIN Q01321 NADH-UBIQUINONE OXIDOREDUCTASE MLRQ SUBUNIT mit einer Identität von 87%.Clone 107 shows homology to Genbank Acc No BE226644.1 JBE226644: ia23h11.y1 Mouse E10 5 12 5 Pancreas cDNA Library Mus musculus cDNA 5 'similar to SW: NUML_BOVIN Q01321 NADH-UBIQUINONE OXIDOREDUCTASE MLRQ SUBUNIT with an identity of 87%.
Klon 108 zeigt Homologie zu Genbank Acc No AF157317.1 |AF157317: Homo sapiens AD-015 protein mRNA, complete cds mit einer Identität von 83%.Clone 108 shows homology to Genbank Acc No AF157317.1 | AF157317: Homo sapiens AD-015 protein mRNA, complete cds with an identity of 83%.
Klon 109 zeigt Homologie zu Genbank Acc No M88136.1 |CRUSTSTA: Cricetulus griseus seryl tRNA synthetase mRNA, partial cds mit einer Identität von 91%.Clone 109 shows homology to Genbank Acc No M88136.1 | CRUSTSTA: Cricetulus griseus seryl tRNA synthetase mRNA, partial cds with an identity of 91%.
Klon 110 keine Homologie zu bekannten Sequenzen.Clone 110 lacks homology to known sequences.
Klon 111 zeigt Homologie zu Genbank Acc No AW106096.27359 j Mm.27359: um23a10.y1 Mus musculus cDNA, 5' end mit einer Identität von 99%. Klon 112 zeigt Homologie zu Genbank Acc No AI413025.1 | AI413025 EST241326 Normalized rat brain, Bento Soares Rattus sp. cDNA cloneRBRDZ283' end mit einer Identität von 97%.Clone 111 shows homology to Genbank Acc No AW106096.27359 j Mm.27359: um23a10.y1 Mus musculus cDNA, 5 'end with an identity of 99%. Clone 112 shows homology to Genbank Acc No AI413025.1 | AI413025 EST241326 Normalized rat brain, Bento Soares Rattus sp. cDNA cloneRBRDZ283 'end with 97% identity.
Klon 113 zeigt Homologie zu EMBL Acc No Human DNA sequence from clone RP3-402G11 on chromosome 22q13.31 -13.33 "Contains the MAPK12 gene for mitogen activated protein kinase 12 (SAPK3), the MAPK11 gene for mitogen activated protein kinase 11 (PRKM11), gene KIAA0315, the gene for a novel protein" mit einer Identität von 86%.Clone 113 shows homology to EMBL Acc No Human DNA sequence from clone RP3-402G11 on chromosome 22q13.31 -13.33 "Contains the MAPK12 gene for mitogen activated protein kinase 12 (SAPK3), the MAPK11 gene for mitogen activated protein kinase 11 (PRKM11) gene KIAA0315, the gene for a novel protein "with an identity of 86%.
Klon 114 zeigt Homologie zu DDBJ Acc No AK000823.11 AK000823 Homo sapiens cDNA FLJ20816 fis, clone ADSE00693 mit einer Identität von 88%.Clone 114 shows homology to DDBJ Acc No AK000823.11 AK000823 Homo sapiens cDNA FLJ20816 fis, clone ADSE00693 with an identity of 88%.
Klon 115 zeigt Homologie zu Genbank Acc No AF007152.11 AF007152 Homo sapiens clone 23649 and 23755 unknown mRNA, partial cds mit einer Identität von 83%.Clone 115 shows homology to Genbank Acc No AF007152.11 AF007152 Homo sapiens clone 23649 and 23755 unknown mRNA, partial cds with an identity of 83%.
Klon 116 zeigt Homologie zu EMBL Acc No X66370.1 JRNRPS9 R. norvegicus mRNA for ribosomal protein S9 mit einer Identität von 94%.Clone 116 shows homology to EMBL Acc No X66370.1 JRNRPS9 R. norvegicus mRNA for ribosomal protein S9 with an identity of 94%.
Klon 117 zeigt Homologie zu Genbank Acc No BE161116.11 BEI 61116 PM3-HT0424- 170200-001-b 11 HT0424 Homo sapiens cDNA mit einer Identität von 100%.Clone 117 shows homology to Genbank Acc No BE161116.11 BEI 61116 PM3-HT0424-170200-001-b 11 HT0424 Homo sapiens cDNA with an identity of 100%.
Klon 118 zeigt Homologie zu DDBJ Acc No AK000559.1 JAK000559 Homo sapiens cDNA FLJ20552 fis, clone KAT11732 mit einer Identität von 86%.Clone 118 shows homology to DDBJ Acc No AK000559.1 JAK000559 Homo sapiens cDNA FLJ20552 fis, clone KAT11732 with an identity of 86%.
Klon 119 zeigt Homologie zu RefSeq Acc No NM_003713.1 | Homo sapiens phosphatidic acid phosphatase type 2b (PPAP2B) mRNA, and translated products mit einer Identität von 87%. Klon 120 zeigt Homologie zu Genbank Acc No M30773.1(HUMCNR: Human calcineurin B mRNA, complete cds mit einer Identität von 88%.Clone 119 shows homology to RefSeq Acc No NM_003713.1 | Homo sapiens phosphatidic acid phosphatase type 2b (PPAP2B) mRNA, and translated products with an identity of 87%. Clone 120 shows homology to Genbank Acc No M30773.1 (HUMCNR: Human calcineurin B mRNA, complete cds with an identity of 88%.
Klon 121 zeigt Homologie zu RefSeq Acc No NM_013770.1 bzw. Genbank Acc. No AF188712.KAF188712: Mus musculus solute carrier family 25 (mitochondrial carrier;adenine nucleotide translocator), member 10 (Slc25a10),mRNA und Mus musculus mitochondrial dicarboxylate carrier mRNA, complete cds; nuclear gene for mitochondrial product mit einer Identität von 98%.Clone 121 shows homology to RefSeq Acc No NM_013770.1 or Genbank Acc. No AF188712.KAF188712: Mus muscle solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 10 (Slc25a10), mRNA and Mus muscle mitochondrial dicarboxylate carrier mRNA, complete cds; nuclear gene for mitochondrial product with an identity of 98%.
Klon 122 zeigt Homologie zu RefSeq Acc No NM_008525.1: Mus musculus delta-aminolevulinate dehydratase (Lv), mRNA mit einer Identität von 100%.Clone 122 shows homology to RefSeq Acc No NM_008525.1: Mus musculus delta-aminolevulinate dehydratase (Lv), mRNA with an identity of 100%.
Klon 123 zeigt Homologie zu Genbank Acc No M59861.KRAT10HCO: Rattus norvegicus 10 formyltetrahydrofolate dehydrogenase mRNA, complete cds mit einer Identität von 93%.Clone 123 shows homology to Genbank Acc No M59861.KRAT10HCO: Rattus norvegicus 10 formyltetrahydrofolate dehydrogenase mRNA, complete cds with an identity of 93%.
Klon 124 zeigt Homologie zu folgenden Datenbankeinträgen: EMBL Acc No X00525.1(MMRNA02: Mouse 28S ribosomai RNA mit einer Identität von 84%; Genbank Acc. No AF061799.KAF061799: Hydrolagus colliei internal transcribed spacer 1, partial sequence;5.8S ribosomai RNA gene and internal transcribed spacer 2, complete sequence; and 28S ribosomai RNA gene, partial-sequence mit einer Identität von 83%; Genbank Acc. No AF061800.1 (AF061800: Squalus acanthias internal transcribed spacer 2; and 28S ribosomai RNA gene, partial sequence mit einer Identität von 83%.Clone 124 shows homology to the following database entries: EMBL Acc No X00525.1 (MMRNA02: Mouse 28S ribosomai RNA with an identity of 84%; Genbank Acc. No AF061799.KAF061799: Hydrolagus colliei internal transcribed spacer 1, partial sequence; 5.8S ribosomai RNA gene and internal transcribed spacer 2, complete sequence; and 28S ribosomai RNA gene, partial-sequence with an identity of 83%; Genbank Acc. No AF061800.1 (AF061800: Squalus acanthias internal transcribed spacer 2; and 28S ribosomai RNA gene, partial sequence with an identity of 83%.
Klon 125 zeigt eine Homologie zu RefSeq Acc. No NM_007469.1: Mus musculus apolipoprotein Cl (Apod), mRNA sowieClone 125 shows homology to RefSeq Acc. No NM_007469.1: Mus muscle apolipoprotein Cl (Apod), mRNA as well
AK011358.1 |AK011358 Mus musculus 10 days embryo cDNA, RIKEN full-length enriched library, clone:261 0009E08, füll insert sequence mit einer Identität von 1 00%.AK011358.1 | AK011358 Mus musculus 10 days embryo cDNA, RIKEN full-length enriched library, clone: 261 0009E08, fill insert sequence with an identity of 1 00%.
Klon 1 27 zeigt eine Homologie zu Genbank Acc. No AK007676: Mus musculus 10 day old male pancreas cDNA, RIKEN full-length enriched library, clone: 1 81 0032P22, füll insert sequence mit einer Identität von 1 00%; zu Genbank Acc. No BC002242: Mus musculus, Similar to sorting nexin 5, clone MGC:7534, mRNA, complete cds mit einer Identität von 100% sowie zu Genbank Acc. No AC008066: Homo sapiens BAC clone RP1 1 -293D9 from 8, complete sequence.Clone 1 27 shows homology to Genbank Acc. No AK007676: Mus musculus 10 day old male pancreas cDNA, RIKEN full-length enriched library, clone: 1 81 0032P22, fill insert sequence with an identity of 1 00%; to Genbank Acc. No BC002242: Mus musculus, Similar to sorting nexin 5, clone MGC: 7534, mRNA, complete cds with an identity of 100% and Genbank Acc. No AC008066: Homo sapiens BAC clone RP1 1 -293D9 from 8, complete sequence.
Klon 1 33 zeigt eine Homologie zu Genbank Acc . No AK01 5068.1 J AK01 5068: Mus musculus adult male testis cDNA, RIKEN full-length enriched library, clone:4930403O06, füll insert sequence mit einer Identität von 90% sowie zu DDBJ Acc. No AK004504.1 1 AK004504: Mus musculus 18 days embryo cDNA, RIKEN full-length enriched library, clone: 1 1 90006A08, füll insert sequenceClone 1 33 shows homology to Genbank Acc. No AK01 5068.1 J AK01 5068: Mus musculus adult male testis cDNA, RIKEN full-length enriched library, clone: 4930403O06, fill insert sequence with an identity of 90% and to DDBJ Acc. No AK004504.1 1 AK004504: Mus musculus 18 days embryo cDNA, RIKEN full-length enriched library, clone: 1 1 90006A08, fill insert sequence
Klon 1 34 zeigt eine Homologie zu Genbank Acc . No BC002108.1 { BC002108: Mus musculus, Similar to ribosomai proteinClone 1 34 shows homology to Genbank Acc. No BC002108.1 {BC002108: Mus musculus, Similar to ribosomai protein
S27a, clone MGC:6601 , mRNA, complete cds mit einer Identität von 98%.S27a, clone MGC: 6601, mRNA, complete cds with 98% identity.
Klon 140 zeigt eine Homologie zu DDBJ Acc. No AK008346.1 | AK008346 Mus musculus adult male small intestine cDNA, RIKEN full-length enriched library, clone:2010107K03, füll insert sequence mit einer identität von 99% sowie zu RefSeq Acc. No NM_007512.1 j Mus musculus ATPase inhibitor (Atpi), mRNA mit einer Identität von 99%.Clone 140 shows homology to DDBJ Acc. No AK008346.1 | AK008346 Mus muscle adult male small intestine cDNA, RIKEN full-length enriched library, clone: 2010107K03, fill insert sequence with an identity of 99% and for RefSeq Acc. No NM_007512.1 j Mus muscle ATPase inhibitor (Atpi), mRNA with an identity of 99%.
Klon 141 zeigt keine Homologie zu bekannten Sequenzen.Clone 141 shows no homology to known sequences.
Klon 1 44 zeigt eine Homologie zu Genbank Acc. No AF006482.1 J AF006482 Mus musculus nucleoside triphosphatase (NTPase) mRNA, complete cds mit einer Identität von 99%; zu Genbank Acc. No AF084569. 1 | AF084569 Mesocricetus auratus cph proto-oncogene product (cph) mRNA, complete cds mit einer Identität von 90%.Clone 1 44 shows homology to Genbank Acc. No AF006482.1 J AF006482 Mus nucleoside triphosphatase muscle (NTPase) mRNA, complete cds with 99% identity; to Genbank Acc. No AF084569. 1 | AF084569 Mesocricetus auratus cph proto-oncogene product (cph) mRNA, complete cds with an identity of 90%.
Klon 145 zeigt eine Homologie zu DDBJ Acc. No AK002787.1 J AK002787 Mus musculus adult male kidney cDNA, RIKEN full-length enriched library, clone:0610038B1 2, füll insert sequence mit einer Identität von 1 00%; zu DDBJ Acc. No AK010989.1 | AK010989 Mus musculus 1 3 days embryo liver cDNA, RIKEN full-length enriched library, clone:2510022D06, füll insert sequence mit einer Identität von 100%; zu RefSeq Acc. No NM_031 101 .1 | Rattus norvegicus ribosomai protein L13 (Rpl13), mRNA mit einer Identität von 93%.Clone 145 shows homology to DDBJ Acc. No AK002787.1 J AK002787 Mus musculus adult male kidney cDNA, RIKEN full-length enriched library, clone: 0610038B1 2, fill insert sequence with an identity of 1 00%; to DDBJ Acc. No AK010989.1 | AK010989 Mus musculus 1 3 days embryo liver cDNA, RIKEN full-length enriched library, clone: 2510022D06, fill insert sequence with an identity of 100%; to RefSeq Acc. No NM_031 101 .1 | Rattus norvegicus ribosomai protein L13 (Rpl13), mRNA with an identity of 93%.
Klon 146 zeigt eine Homologie zu Refseq Acc. No NM_025278.1 | Mus musculus guanine nucleotide binding protein (G protein), gamma 1 2 (Gng1 2), mRNA mit einer Identität von 99%.Clone 146 shows homology to Refseq Acc. No NM_025278.1 | Mus muscle guanine nucleotide binding protein (G protein), gamma 1 2 (Gng1 2), mRNA with an identity of 99%.
Die durch den genetischen Screen identifizierten Apoptose-induzierenden Gene (Klone 1 - 1 24) sind in der als Abbildung beigefügten Tabelle 1 (Seiten 1 - 125) aufgelistet:The apoptosis-inducing genes identified by the genetic screen (clones 1 - 1 24) are listed in Table 1 (pages 1 - 125) attached as an illustration:
Die Angaben in Tabelle 1 sind wie folgt definiert:The information in Table 1 is defined as follows:
"T7-Sequenz": 5'-seitige Sequenz des Klons "BGH-Sequenz": 3'-seitige Sequenz des Klons"T7 sequence": 5 'side sequence of the clone "BGH sequence": 3' side sequence of the clone
"interner Primer": interne Sequenzen des Klons identifiziert unter Verwendung von Primern erhalten aus der T7-Sequenz (links) bzw. der BGH-Sequenz (rechts) "Identität" : Vergleich mit Sequenzen aus dem Computerprogramm"Internal primer": internal sequences of the clone identified using primers obtained from the T7 sequence (left) or the BGH sequence (right) "identity": comparison with sequences from the computer program
"BLAST" (Altschul et al. (1 997), Nucleic Acids Res. 25:3389-3402). Neben völlig identischen Sequenzen (Identität 1 00 %) sind auch teilidentische Sequenzen (Identität von vorzugsweise ≥ 85 %) angegeben, die allelische Varianten der konkret gezeigten Sequenz bzw. homologe Sequenzen aus anderen Spezies, insbesondere aus dem Menschen, zeigen."BLAST" (Altschul et al. (1,997), Nucleic Acids Res. 25: 3389-3402). In addition to completely identical sequences (Identity 100%) partially identical sequences (identity of preferably ≥ 85%) are also given, which show allelic variants of the specifically shown sequence or homologous sequences from other species, in particular from humans.
Derartige Varianten bzw. homologe Sequenzen werden selbstverständlich ebenfalls von der vorliegenden Erfindung erfasst.Such variants or homologous sequences are of course also covered by the present invention.
"contig" vollständige Sequenz; zusammengefügt aus der „T7-Sequenz", „BGH-Sequenz sowie gegebenenfalls aus den „internen Primer"-Sequenzen"contig" complete sequence; put together from the "T7 sequence", "BGH sequence and optionally from the" internal primer "sequences
"Eigenschaften' Angaben zu prognostizierten Eigenschaften des resultierenden Peptids"Properties" Information about the predicted properties of the resulting peptide
,Vorhersage" Eine Analyse mit dem Computerprogramm „PSORT II" (Nakai, K. and Kanehisa, M., Genomics 14: 897-91 1 ( 1 992)) bestimmt für das resultierende Peptid anhand der Sequenz mögliche Eigenschaften sowie die Lokalisation in der Zelle mit einer prognostizierten Wahrscheinlichkeit. Für den Klon 1 werden beispielsweise zwei Transmembrandomänen sowie eine Lokalisation im ER (Endoplasmatischen Retikulum) mit einer Wahrscheinlichkeit von 33%, in der Vakuole mit einer Wahrscheinlichkeit von 22%, in den Mitochondrien mit einer Wahrscheinlichkeit von 1 1 % und im Golgiapparat mit einer Wahrscheinlichkeit von 1 1 % bestimmt „DNA-Leiter" Biochemischer Nachweis für Apoptose (Bitzer et al., Prediction "An analysis with the computer program" PSORT II "(Nakai, K. and Kanehisa, M., Genomics 14: 897-91 1 (1 992)) determines possible properties and the localization in the resulting peptide from the sequence Cell with a predicted probability. For clone 1, for example, two transmembrane domains and a location in the ER (endoplasmic reticulum) with a probability of 33%, in the vacuole with a probability of 22%, in the mitochondria with a probability of 1 1% and in the Golgi apparatus with a probability determined by 1 1% "DNA leader" Biochemical detection for apoptosis (Bitzer et al.
(1999) J. Virol. 73: 702-708)(1999) J. Virol. 73: 702-708)
„Pl-Facs„ Propidium lodid-Facs": Nachweis für Apoptose (Bitzer et al. (1999) J. Virol. 73: 702-708)"PI-Facs" Propidium iodide-Facs ": Detection for apoptosis (Bitzer et al. (1999) J. Virol. 73: 702-708)
„möglicher ORF" Angabe eines möglichen „open reading frames" (ORF) im Ein-Buchstaben-Code"Possible ORF" Specification of a possible "open reading frame" (ORF) in the one-letter code
Die durch den genetischen Screen identifizierten Apoptose-induzierten Gene (Klone 1 -124, 125, 127, 133, 134, 140, 141 , 144, 145, 146) sind in der als Abbildung beigefügten Tabelle 2 (Seiten 1 - 28) aufgelistet. The apoptosis-induced genes identified by the genetic screen (clones 1-124, 125, 127, 133, 134, 140, 141, 144, 145, 146) are listed in Table 2 (pages 1-28) attached as an illustration.
Tabelle 1Table 1
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Konvention für Bewertung des phänotypischen 'Zelltod-Effektes':Convention for the evaluation of the phenotypic 'cell death effect':
- negativ, -/+ und -/++ schwach positiv, + positiv, ++ stark positiv, +++ sehr stark positiv- negative, - / + and - / ++ weakly positive, + positive, ++ strongly positive, +++ very strongly positive
Klon #53Clone # 53
T7 -Sequenz GCTTGACCTTGGAGGCCTCAGCCTGAGACCTCAAAGCAGCCTCCAGAACTCCGGCAGAGTTCCTCTGTCTCGTCTTGCCGT7 sequence GCTTGACCTTGGAGGCCTCAGCCTGAGACCTCAAAGCAGCCTCCAGAACTCCGGCAGAGTTCCTCTGTCTCGTCTTGCCG
ATTGMGGTCCCCGTTTCTCC TTTCTCTCCATCTTCTGGGAGGTAGCAGGAAATCAGAATCATGGTTGGTTTCAAGGCCATTGMGGTCCCCGTTTCTCC TTTCTCTCCATCTTCTGGGAGGTAGCAGGAAATCAGAATCATGGTTGGTTTCAAGGCC
CAGATGTGCCCCCAACAGCCACTGTGAAGTTCCTGGGGGCTGGGACAGCTGCCTGCATTGCAGATCTCATCACTTTCCCTCAGATGTGCCCCCAACAGCCACTGTGAAGTTCCTGGGGGCTGGGACAGCTGCCTGCATTGCAGATCTCATCACTTTCCCT
CTGGATACCGCCAAGGTCCGGCTGCAGATCCAAGGGGAGAGTCAAGGGCTAGTGCGCACCGCAGCCAGCGCCCAGTACCTGGATACCGCCAAGGTCCGGCTGCAGATCCAAGGGGAGAGTCAAGGGCTAGTGCGCACCGCAGCCAGCGCCCAGTAC
GTGGCGTTCTGGGTACCATCCTAACCATGGTGCGCACTGAGGGTCCACGCAGCCTCTACAATGGGCTGGACGCCGGCCTGTGGCGTTCTGGGTACCATCCTAACCATGGTGCGCACTGAGGGTCCACGCAGCCTCTACAATGGGCTGGACGCCGGCCT
GCAGCGCCAGATGAGCTTTGCCTCCGTCCGCATTGGCCTCTACGACTCTGTCAAACAGTTCTACACCAAGGGCTCAGAGCGCAGCGCCAGATGAGCTTTGCCTCCGTCCGCATTGGCCTCTACGACTCTGTCAAACAGTTCTACACCAAGGGCTCAGAGC
ATGCAGGCATCGNGAGCCGCTTCTGGCAGGTAGCACCACAGGTGCCTTGNCGTGGCTGTNGCCCNAGCCTACAGATGTNATGCAGGCATCGNGAGCCGCTTCTGGCAGGTAGCACCACAGGTGCCTTGNCGTGGCTGTNGCCCNAGCCTACAGATGTN
NAAAGGCCCGTTTC NAAAGGCCCGTTTC
BGH-Sequenz: GCCAGCCTAACACTTCTATGACAAACCGANGAAAATTATAAAAACTTTTCAAATATATTGCTCCTCAGACCCCCTATCCACABGH sequence: GCCAGCCTAACACTTCTATGACAAACCGANGAAAATTATAAAAACTTTTCAAATATATTGCTCCTCAGACCCCCTATCCACA
CATTAATTATTTTAACAGCCTGATTACTGCCACTAATATTAATAGCTAGCCAA CCACCTAAAAAAAGATAATAACNGTACTCATTAATTATTTTAACAGCCTGATTACTGCCACTAATATTAATAGCTAGCCAA CCACCTAAAAAAAGATAATAACNGTACT
CAAA CTCTACATCTC TACT TCAGCTTACAAATTCTCCT TCATMCCTTTTCAGCAACTGAACTAATTATATTTTATCAAA CTCTACATCTC TACT TCAGCTTACAAATTCTCCT TCATMCCTTTTCAGCAACTGAACTAATTATATTTTAT
TTTTATTTGAAGCAACCTTAATCCCAACACTTATTATTATTACCCGATGAGGGAACCAAACTGAACGCCTAAACGCAGGGATTTTTATTTGAAGCAACCTTAATCCCAACACTTATTATTATTACCCGATGAGGGAACCAAACTGAACGCCTAAACGCAGGGAT
TATTTCCTATTTTATACCCTAATCGGTTCTATTCCACTGCTAATTGCCCTCATCTTAATCCAAAACCATGTAGGAACCCTAAATATTTCCTATTTTATACCCTAATCGGTTCTATTCCACTGCTAATTGCCCTCATCTTAATCCAAAACCATGTAGGAACCCTAAA
CTCATMTTTTATCATTCACAACACACACCTTAGACGCTTCATGATCTAACAACTTACTATGGTTGGCATGCATAATAGCATTCTCATMTTTTATCATTCACAACACACACCTTAGACGCTTCATGATCTAACAACTTACTATGGTTGGCATGCATAATAGCATT
CTTATTANAATACCATTATATGGAGTTCACCTATGACTACCAAAAGCCCATGTTGAAGCTCCAATTGCTGGGTCAATAATTCTCTTATTANAATACCATTATATGGAGTTCACCTATGACTACCAAAAGCCCATGTTGAAGCTCCAATTGCTGGGTCAATAATTCT
AGCAGCTATTCTTCTAAAATTAGGTAGTTACGGAATAATTCGCATCTCCAGCAGCTATTCTTCTAAAATTAGGTAGTTACGGAATAATTCGCATCTCC
Laufende Klon Nummer: #7 ACCESS No.: M27315.1 GL343181 Definition: Rattus norvegicus mitochondrial cytochrome c oxidase subunits I, II and III, and ATPase subunit 6 genes, complete cds, and Trp-, Ala- Asn-, Cys-, Tyr-, Ser-, Asp-, Lys-, Gly-, Arg-tRNA genes.Running clone number: # 7 ACCESS No .: M27315.1 GL343181 definition: Rattus norvegicus mitochondrial cytochrome c oxidase subunits I, II and III, and ATPase subunit 6 genes, complete cds, and Trp-, Ala- Asn-, Cys-, Tyr, Ser, Asp, Lys, Gly, Arg tRNA genes.
LOCUS: RATMTCYTOC 7632 bp DNA ROD 29-MAY-1996 Effekt: ++LOCUS: RATMTCYTOC 7632 bp DNA ROD 29-MAY-1996 Effect: ++
Klon #55 Clone # 55
T7-Sequenz GGATTGGCGGGGTTCTCTTTGACCACATAAATCAATTCTTTCTTTTGGGAGCCTCAATGGTGGCAACTGCAGCGGGACTTTT7 sequence GGATTGGCGGGGTTCTCTTTGACCACATAAATCAATTCTTTCTTTTGGGAGCCTCAATGGTGGCAACTGCAGCGGGACTTT
ATCTCATCCCGTTCTGCAAGACAGCAGTCTTATTGATCATCACCATGTCTGTCTTTGGAGCTTCCGTTGGTGTTGTGGATACATCTCATCCCGTTCTGCAAGACAGCAGTCTTATTGATCATCACCATGTCTGTCTTTGGAGCTTCCGTTGGTGTTGTGGATAC
AGGTGCAAATGTTCTCATCTTGGATCTCTGGGGGGACAAAGGAGCCCCACAAATGCAGGCCTTGCACTTCAGTTTCGCCTTAGGTGCAAATGTTCTCATCTTGGATCTCTGGGGGGACAAAGGAGCCCCACAAATGCAGGCCTTGCACTTCAGTTTCGCCTT
GGGTGCCTTTCTGGCTCCCCTGCTGGCTAAGTTGGCCTGGGGTACAGCACCTGCTCAGAACCACACCGAGTCCGACCTTGGGGTGCCTTTCTGGCTCCCCTGCTGGCTAAGTTGGCCTGGGGTACAGCACCTGCTCAGAACCACACCGAGTCCGACCTTG
ACACTCTGATGCTGAACCGATCCTCCAACGGCACCTCAGACTCCGTGTTTGCGGTACCCGATGACATGAATCTGCTGNGGACACTCTGATGCTGAACCGATCCTCCAACGGCACCTCAGACTCCGTGTTTGCGGTACCCGATGACATGAATCTGCTGNGG
GCATATGCTTCCATTGGCACCTTTATTTTAGTAGTTTCTGTCTTTCTGNNNGGTCTGTTTTGTAAGAAACATTCAAGGCAGAAGCATATGCTTCCATTGGCACCTTTATTTTAGTAGTTTCTGTCTTTCTGNNNGGTCTGTTTTGTAAGAAACATTCAAGGCAGAA
AAAACCCAGAGCATCTGCTGAGGGAGCTCGAAGGGCTAAATATCACAGGGCCCTGCTATGCCTGCTCTTCCTCTTCTTCTTAAAACCCAGAGCATCTGCTGAGGGAGCTCGAAGGGCTAAATATCACAGGGCCCTGCTATGCCTGCTCTTCCTCTTCTTCTT
CTTCTATGTGGGAGCCGAGATAACATACGGCTCTTACATATTCTCCTTCGCCACCACCCATGTTGGCATGGAAGAGAGCGACTTCTATGTGGGAGCCGAGATAACATACGGCTCTTACATATTCTCCTTCGCCACCACCCATGTTGGCATGGAAGAGAGCGA
GGCAGCTGGCTTGAATTCCATCTTCTGGGGAACCTTTGCAGCCTGCAGGGGCCTGGCCATCTTCTTTGCGACATTCTTACAGGCAGCTGGCTTGAATTCCATCTTCTGGGGAACCTTTGCAGCCTGCAGGGGCCTGGCCATCTTCTTTGCGACATTCTTACA
GCCTGGAACCATGATCGTGCTGAGCAACATTGGCAGCCTGGNCTCATGTTTCTTTCTGGTACTGCCTGGAACCATGATCGTGCTGAGCAACATTGGCAGCCTGGNCTCATGTTTCTTTCTGGTACT
BGH-Sequenz: AAATATCACA GGGCCNTGNT AGCCTGCTNT TCCCTCTTCT TCTTCTTCTA NTGGAANCCG AAGNTACCAT NNCGNTTCTT ANCAGTTTCT CCTTTCGGCC NCCNACCCAG TGGCATGGAA GAGAGCGAGG CAGCTGGCTT GAATTCCCAT CTTCTGGGGA ACCTTTGCAG CCTGCAGGGG CCTGGCCATC TTCTTTGCGA CATTTCTTAC AGCCTGGANC CATGATCGTG CTGAGCAACA TTGGCAGCCT GGTCTCATGT TTCTTTCTGG TACTTTTTGA CAAGAGCCCT CTTTGTCTCT GGATCGCGAC TTCTGTGTAT GGAGCCTCAA TGGCAGCCAC GTTTCCCAGC GGCATCTCCT GGATTGAGCA GTACNCCNCC TTAACTGGGA AATCTGCAGC ATTCTTTGTA ATTGGCTCTG CCCTGGGAGA TATGGCCATT CCAGCGGTGA TCGGAATTCT TCAGGGACNC TACCCAGATC TGCCAGTAGT TCTGTACACA TGTCTGGGCT CAGCCATATT CACAGCTATT TTATTTCCTG TGATGTATAA ATTAGCTACC TTGCCCCTGA AGAGAGAGGA CCAGAAAGCT TTGCCCACTA GTTCTAGACT GTGAGGAAGA GACTACATGA GAACTTAAAA AAAAAAAAAA AGGGCGGCCG CTCGAGCATG CATCTAGAGG GCCCTATTCT ATAGTGTCAC CTAAATGCTA GAGCNCNNCT GTCA BGH sequence: AAATATCACA GGGCCNTGNT AGCCTGCTNT TCCCTCTTCT TCTTCTTCTA NTGGAANCCG AAGNTACCAT NNCGNTTCTT ANCAGTTTCT CCTTTCGGCC NCCNACCCAG TGGCATGGAA GAGAGCGAGG CAGCTGGCTT GAATTCCCAT CTTCTGGGGA ACCTTTGCAG CCTGCAGGGG CCTGGCCATC TTCTTTGCGA CATTTCTTAC AGCCTGGANC CATGATCGTG CTGAGCAACA TTGGCAGCCT GGTCTCATGT TTCTTTCTGG TACTTTTTGA CAAGAGCCCT CTTTGTCTCT GGATCGCGAC TTCTGTGTAT GGAGCCTCAA TGGCAGCCAC GTTTCCCAGC GGCATCTCCT GGATTGAGCA GTACNCCNCC TTAACTGGGA AATCTGCAGC ATTCTTTGTA ATTGGCTCTG CCCTGGGAGA TATGGCCATT CCAGCGGTGA TCGGAATTCT TCAGGGACNC TACCCAGATC TGCCAGTAGT TCTGTACACA TGTCTGGGCT CAGCCATATT CACAGCTATT TTATTTCCTG TGATGTATAA ATTAGCTACC TTGCCCCTGA AGAGAGAGGA CCAGAAAGCT TTGCCCACTA GTTCTAGACT GTGAGGAAGA GACTACATGA GAACTTAAAA AAAAAAAAAA AGGGCGGCCG CTCGAGCATG CATCTAGAGG GCCCTATTCT ATAGTGTCAC CTAAATGCTA GAGCNCNNCT GTCA
Gesamt-Sequenz GGATTGGCGGGGTTCTCTTTGACCACATAAATCAATTCTTTCTTTTGGGAGCCTCAATGGTGGCAACTGC zusammengesetzt (aus T7 AGCGGGACTTTATCTCATCCCGTTCTGCAAGACAGCAGTCTTATTGATCATCACCATGTCTGTCTTTGGA und BGH) GCTTCCGTTGGTGTTGTGGATACAGGTGCAAATGTTCTCATCTTGGATCTCTGGGGGGACAAAGGAGCCCOverall sequence GGATTGGCGGGGTTCTCTTTGACCACATAAATCAATTCTTTCTTTTGGGAGCCTCAATGGTGGCAACTGC composed (from T7 AGCGGGACTTTATCTCATCCCGTTCTGCAAGACAGCAGTCTTATTGATCATCACCATGTCTGTCTTTGGA and BGH) GCTTCCGTTGGTGTTGTGGATACAGGTGCAAATGTTCTCATCTTGGATCTCTGGGGGGACAAAGGAGCCC
CACAAATGCAGGCCTTGCACTTCAGTTTCGCCTTGGGTGCCTTTCTGGCTCCCCTGCTGGCTAAGTTGGCCACAAATGCAGGCCTTGCACTTCAGTTTCGCCTTGGGTGCCTTTCTGGCTCCCCTGCTGGCTAAGTTGGC
CTGGGGTACAGCACCTGCTCAGAACCACACCGAGTCCGACCTTGACACTCTGATGCTGAACCGATCCTCCCTGGGGTACAGCACCTGCTCAGAACCACACCGAGTCCGACCTTGACACTCTGATGCTGAACCGATCCTCC
AACGGCACCTCAGACTCCGTGTTTGCGGTACCCGATGACATGAATCTGCTGNGGGCATATGCTTCCATTGAACGGCACCTCAGACTCCGTGTTTGCGGTACCCGATGACATGAATCTGCTGNGGGCATATGCTTCCATTG
GCACCTTTATTTTAGTAGTTTCTGTCTTTCTGNNNGGTCTGTTTTGTAAGAAACATTCAAGGCAGAAAAAGCACCTTTATTTTAGTAGTTTCTGTCTTTCTGNNNGGTCTGTTTTGTAAGAAACATTCAAGGCAGAAAAA
ACCCAGAGCATCTGCTGAGGGAGCTCGAAGGGCTAAATATCACAGGGCCCTGCTATGCCTGCTCTTCCTCACCCAGAGCATCTGCTGAGGGAGCTCGAAGGGCTAAATATCACAGGGCCCTGCTATGCCTGCTCTTCCTC
TTCTTCTTCTTCTATGTGGGAGCCGAGATAACATACGGCTCTTACATATTCTCCTTCGCCACCACCCATGTTCTTCTTCTTCTATGTGGGAGCCGAGATAACATACGGCTCTTACATATTCTCCTTCGCCACCACCCATG
TTGGCATGGAAGAGAGCGAGGCAGCTGGCTTGAATTCCATCTTCTGGGGAACCTTTGCAGCCTGCAGGGGTTGGCATGGAAGAGAGCGAGGCAGCTGGCTTGAATTCCATCTTCTGGGGAACCTTTGCAGCCTGCAGGGG
CCTGGCCATCTTCTTTGCGACATTCTTACAGCCTGGAACCATGATCGTGCTGAGCAACATTGGCAGCCTGCCTGGCCATCTTCTTTGCGACATTCTTACAGCCTGGAACCATGATCGTGCTGAGCAACATTGGCAGCCTG
GTCTCATGTTTCTTTCTGGTAC I I I I 1 GACAAGAGCCCTCTTTGTCTCTGGATCGCGACTTCTGTGTATGGTCTCATGTTTCTTTCTGGTAC I I I I 1 GACAAGAGCCCTCTTTGTCTCTGGATCGCGACTTCTGTGTATG
GAGCCTCAATGGCAGCCACGTTTCCCAGCGGCATCTCCTGGATTGAGCAGTACNCCNCCTTAACTGGGAAGAGCCTCAATGGCAGCCACGTTTCCCAGCGGCATCTCCTGGATTGAGCAGTACNCCNCCTTAACTGGGAA
ATCTGCAGCATTCTTTGTAATTGGCTCTGCCCTGGGAGATATGGCCATTCCAGCGGTGATCGGAATTCTTATCTGCAGCATTCTTTGTAATTGGCTCTGCCCTGGGAGATATGGCCATTCCAGCGGTGATCGGAATTCTT
CAGGGACNCTACCCAGATCTGCCAGTAGTTCTGTACACATGTCTGGGCTCAGCCATATTCACAGCTATTTCAGGGACNCTACCCAGATCTGCCAGTAGTTCTGTACACATGTCTGGGCTCAGCCATATTCACAGCTATTT
TATTTCCTGTGATGTATAAATTAGCTACCTTGCCCCTGAAGAGAGAGGACCAGAAAGCTTTGCCCACTAGTATTTCCTGTGATGTATAAATTAGCTACCTTGCCCCTGAAGAGAGAGGACCAGAAAGCTTTGCCCACTAG
TTCTAGACTGTGAGGAAGAGACTACATGAGAACTTAAAAAAAAAAAAAAAGGGCGGCCGCTCGAGCATGCTTCTAGACTGTGAGGAAGAGACTACATGAGAACTTAAAAAAAAAAAAAAAGGGCGGCCGCTCGAGCATGC
ATCTAGAGGGCCCTATTCTATAGTGTCACCTAAATGCTAGAGCNCNNCTGTCAATCTAGAGGGCCCTATTCTATAGTGTCACCTAAATGCTAGAGCNCNNCTGTCA
Laufende Klon Nummer: #13 ACCESS No.: AL080317.11 Gϊ.5830430 Definition: Human DNA sequence from äone RP5-1 12D6 on chromosome 6q2 -22.2. Contains the gene for a PUTATIVE novel protein similar to bacterial NARK (nitrite extrusion protein, nitrite facilitator), the 3' end of the REV3L gene for REV3 (yeast homolog)-like, catalytic subunit of DNA polymerase zeta (EC 2.7.7.7, POLZ), ESTs, STSs, GSSs and a putative CpG island, complete sequence.Running clone number: # 13 ACCESS No .: AL080317.11 Gϊ.5830430 Definition: Human DNA sequence from äone RP5-1 12D6 on chromosome 6q2 -22.2. Contains the gene for a PUTATIVE novel protein similar to bacterial NARK (nitrite extrusion protein, nitrite facilitator), the 3 'end of the REV3L gene for REV3 (yeast homolog) -like, catalytic subunit of DNA polymerase zeta (EC 2.7.7.7, POLZ), ESTs, STSs, GSSs and a putative CpG island, complete sequence.
LOCUS HSJ1112D6 135305 bp DNA PRI 21-FEB-2000 Effekt: LOCUS HSJ1112D6 135305 bp DNA PRI 21-FEB-2000 Effect:
Laufende Klon Nummer: #20 (EST) ACCESS No.: AW106096.1 Gl:6076832 Definition: um23a10.y1 Sugano mouse embryo mewa Mus musculus cDNA cloneRunning clone number: # 20 (EST) ACCESS No .: AW106096.1 Gl: 6076832 Definition: um23a10.y1 Sugano mouse embryo mewa Mus musculus cDNA clone
IMAGE:2225370 5', mRNA sequenceIMAGE: 2225370 5 ', mRNA sequence
LOCUS: AW106096 539 bp mRNA EST 20-OCT-1999LOCUS: AW106096 539 bp mRNA EST 20-OCT-1999
Effekt: ++Effect: ++
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Klon #57Clone # 57
T7-Sequenz GATAGTTTTGAACTTCCGATTCCCCTGCCTCCCCACCCCAGGGCTGTGATTGCAGGTGTGCGCTTGGGACTGACCCCAGT7 sequence GATAGTTTTGAACTTCCGATTCCCCTGCCTCCCCACCCCAGGGCTGTGATTGCAGGTGTGCGCTTGGGACTGACCCCAG
CTTTGTCGGTGCTAGGGCAGCACTCTGCGACTGMTTAGGTCCCAGCCACTTCTCTGTCTTTTAAAAGAACAAAACATTGCCTTTGTCGGTGCTAGGGCAGCACTCTGCGACTGMTTAGGTCCCAGCCACTTCTCTGTCTTTTAAAAGAACAAAACATTGC
AAATGTGCCATTGTTGCTTTGAGTTTTAATTCTTTTTTTTTTCTTTCTTTCATAAAACAAATGTGCCATTGTTGCTTTGAGTTTTAATTCTTTTTTTTTTCTTTCTTTCATAAAAC
TATTCTGGTTCTATTΓCTGTCTTTTCACTCAAAACTGGTTTTACAAATGATGCCTTGTTTACAGAAAGCTCTCTACCACAGGGTATTCTGGTTCTATTΓCTGTCTTTTCACTCAAAACTGGTTTTACAAATGATGCCTTGTTTACAGAAAGCTCTCTACCACAGGG
CCTAGTCATGTGTAAAGTCTCAGTTTCTCTCTGGAGTATCTTGGAGCCTAGCACACTGGCTTTAAAGGACACAGCTAAGAACCTAGTCATGTGTAAAGTCTCAGTTTCTCTCTGGAGTATCTTGGAGCCTAGCACACTGGCTTTAAAGGACACAGCTAAGAA
CTGATATCTTGACAGTGTTTGTAGACCTTTGTTATAAAAATGAATGTCCTGGAAAGGGTTGGGAGGGAGTTCAACAACAAACTGATATCTTGACAGTGTTTGTAGACCTTTGTTATAAAAATGAATGTCCTGGAAAGGGTTGGGAGGGAGTTCAACAACAAA
AAACAAGMTGTCATGTTTAAATTTAATAGTTGTCTAAAATGTCATCTC GTCAAGTCACTGGTCTGTTTGCATTTGATAGGAAACAAGMTGTCATGTTTAAATTTAATAGTTGTCTAAAATGTCATCTC GTCAAGTCACTGGTCTGTTTGCATTTGATAGG
TTTTATACTAACTAGCATTATAAGATTATTTCATAATTAGAAAATACCTGTGGATATTTGTATAAAAGTGTGAAATAAAT^TTTTATACTAACTAGCATTATAAGATTATTTCATAATTAGAAAATACCTGTGGATATTTGTATAAAAGTGTGAAATAAAT ^
ACAAAAGTGCTCATCGCTTGTTAACACAGCATCATGTATGTGAAAGCAAACTCTAAGATTATAAATGACAACCTGAGTTGCCACAAAAGTGCTCATCGCTTGTTAACACAGCATCATGTATGTGAAAGCAAACTCTAAGATTATAAATGACAACCTGAGTTGCC
TTCTTΓGTATTTCATCAAGCCNAAGTAAAGCTTTCANTATTTAAA TTCTTΓGTATTTCATCAAGCCNAAGTAAAGCTTTCANTATTTAAA
BGH-Sequenz: n.d.BGH sequence: n.d.
Laufende Klon Nummer: #21Running clone number: # 21
EST mit geringer Homologie zu humanem IntegrinEST with low homology to human integrin
ACCESS No.: NM_000210.1 Gl:4557674ACCESS No .: NM_000210.1 Gl: 4557674
Definition: Homo sapiens integrin, alpha 6 (ITGA6) mRNA.Definition: Homo sapiens integrin, alpha 6 (ITGA6) mRNA.
LOCUS: NM_000210 5611 bp mRNA PRI 15-MAY-2000LOCUS: NM_000210 5611 bp mRNA PRI 15-MAY-2000
Effekt:Effect:
Klon #58 Clone # 58
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Klon #59Clone # 59
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Klon #60 T7-Sequeπz GCATCCCTCCATGGCCTCTGCATTGGCTTCTGCTTTCTGACCTGCTTGAGTTCCAGTCCTGACTTCCTTGGNGATGAACAGClone # 60 T7 sequence GCATCCCTCCATGGCCTCTGCATTGGCTTCTGCTTTCTGACCTGCTTGAGTTCCAGTCCTGACTTCCTTGGNGATGAACAG
CAGTATGGAAGTGTAAGCCGMTAAACCCTTTCCTCTCCAACTTGTTTCTTGGTCATGATGNNTGTGCAGGAATAGAAACCCAGTATGGAAGTGTAAGCCGMTAAACCCTTTCCTCTCCAACTTGTTTCTTGGTCATGATGNNTGTGCAGGAATAGAAACC
TGACT GACAAATTGGTACCAGCAGAGTGGGGTATTCCTGTGACAACCTGACCATGTTTTGGGGAGGACTGTGGATGGATGACT GACAAATTGGTACCAGCAGAGTGGGGTATTCCTGTGACAACCTGACCATGTTTTGGGGAGGACTGTGGATGGA
TTTGGAACTTTGGGCTTAAAGATCCATCCGNTGNTAAGAGCTCTGTCAGATGTTGTGTAGGAGCTTGGAAGATAATGTTGATTTGGAACTTTGGGCTTAAAGATCCATCCGNTGNTAAGAGCTCTGTCAGATGTTGTGTAGGAGCTTGGAAGATAATGTTGA
AACACTGNAGAANATGGAGGNCTGGTNTGNGAAATTTNAGAGGGAAAANTAAAGACTCTTTCAGGGCCATTGCTGTTTTGAAACACTGNAGAANATGGAGGNCTGGTNTGNGAAATTTNAGAGGGAAAANTAAAGACTCTTTCAGGGCCATTGCTGTTTTGA
ATGTGAAGATTCTGTAGNNCTGGATAGCTGGGGCTNAANAATCANCTGTGNTTAACANGATCCAGAACTACCAANGCANAAATGTGAAGATTCTGTAGNNCTGGATAGCTGGGGCTNAANAATCANCTGTGNTTAACANGATCCAGAACTACCAANGCANAA
ACTTTGCATTACTGGGACTATTGATGCNTNNNTAGCTGNAGCTAACAAATTANCGGTGATTAANAAAGAGACCAGCNTCATTACTTTGCATTACTGGGACTATTGATGCNTNNNTAGCTGNAGCTAACAAATTANCGGTGATTAANAAAGAGACCAGCNTCATT
NAGGGTGACATCTTTCTGGGNANGTGTTTCNTGAAAGCACANNGATNCNTGTGNTTNCAGAGATGGCCAAANGCTGTACCNAGGGTGACATCTTTCTGGGNANGTGTTTCNTGAAAGCACANNGATNCNTGTGNTTNCAGAGATGGCCAAANGCTGTACC
CCTGCTTGCAANNANGGACTTGGGNATAANTGTAANNGGGTCNACCCNCNTNGNTACTGGGGTTTTTTAANGGCCNTTTACCTGCTTGCAANNANGGACTTGGGNATAANTGTAANNGGGTCNACCCNCNTNGNTACTGGGGTTTTTTAANGGCCNTTTA
CNGGGNTCNACGGCCACAGNCANCCTTCAGGGCCTNNGGCANCCTGGTTAAAAANGCCCCATTNNGAAANGGNCCAAATCNGGGNTCNACGGCCACAGNCANCCTTCAGGGCCTNNGGCANCCTGGTTAAAAANGCCCCATTNNGAAANGGNCCAAAT
GNCTTTNAAAGGGTACNAGGCCTNCAAACTTGCCCAAATTGTANAGGGCCNCCCGGGGNCTTTNAAAGGGTACNAGGCCTNCAAACTTGCCCAAATTGTANAGGGCCNCCCGGG
BGH-Sequenz: GACCTGGCTT TNGCNCGCNN TNCANCCCTA GTCCCCCGGG TTCTCACTCC CTAAGCCCAT CGCAGNCCGG NTNGTGGANC CGCGCGTCCC AGGNTTCGTC CTTNCNCGGC CTNCAAGAAC ATGGCTTGCT TCAGAAAGAA AATAGTTTTG TCTTCTCTAA NAACTTACNT TCAGCTTGTC GAAGATGAAA ATAAAAAGCC CTGGAGAGGA ATAATTTCTT GCNCTTTATG AATCTATTTT TAAAATAAAA AAATTTACCN NCTTTNAATC TTTTTCCTCC TCNCAAAAGN AACCAGTATT TTTGCCTNCC ATTCANTTTG CNNCANTAAG ANNTTTGGAG CCTGAAACCN NAGNCTTTNT NANGGANTNT CNCCTTGGTT CAGCCTGNAG GCAAATCTGA TCAACGGACC TTTATGAGTC ATTTTTCCTA GACATATTCA GAAAACCTAG GAGCTGTGTC AAATGCCTGA ATTAAGCATT ACAAATGCAA GATNTTTGCN CTCTTGAAGA ATGTAGAGAG TAAAAGAACT ANAATTAAAA ANAATAANGC NTGTGATATA ACGGAATATA TATNTNAAAA ANAA BGH sequence: GACCTGGCTT TNGCNCGCNN TNCANCCCTA GTCCCCCGGG TTCTCACTCC CTAAGCCCAT CGCAGNCCGG NTNGTGGANC CGCGCGTCCC AGGNTTCGTC CTTNCNCGGC CTNCAAGAAC ATGGCTTGCT TCAGAAAGAA AATAGTTTTG TCTTCTCTAA NAACTTACNT TCAGCTTGTC GAAGATGAAA ATAAAAAGCC CTGGAGAGGA ATAATTTCTT GCNCTTTATG AATCTATTTT TAAAATAAAA AAATTTACCN NCTTTNAATC TTTTTCCTCC TCNCAAAAGN AACCAGTATT TTTGCCTNCC ATTCANTTTG CNNCANTAAG ANNTTTGGAG CCTGAAACCN NAGNCTTTNT NANGGANTNT CNCCTTGGTT CAGCCTGNAG GCAAATCTGA TCAACGGACC TTTATGAGTC ATTTTTCCTA GACATATTCA GAAAACCTAG GAGCTGTGTC AAATGCCTGA ATTAAGCATT ACAAATGCAA GATNTTTGCN CTCTTGAAGA ATGTAGAGAG TAAAAGAACT ANAATTAAAA ANAATAANGC NTGTGATATA ACGGAATATA TATNTNAAAA ANAA
Klon #62Clone # 62
T7-Sequenz GCAAACATGGNCAGGAGCATCTTGGCAGCNTTAAGCCTTCANAGAATTATCAACCANGGNCATNAGAGNCGACTCTGNCTCT7 sequence GCAAACATGGNCAGGAGCATCTTGGCAGCNTTAAGCCTTCANAGAATTATCAACCANGGNCATNAGAGNCGACTCTGNCTC
NTTCAGGCTTGCACCACCACCACAGGCNTAGGGGCCAGCACAGGCAGGGTCACTTANAGAGCTGAGACACCACAGCAAGNTTCAGGCTTGCACCACCACCACAGGCNTAGGGGCCAGCACAGGCAGGGTCACTTANAGAGCTGAGACACCACAGCAAG
NGAAGGCTNGCACCTTTCACTTGCCCAGAGGANGCTCTGACNAAGGGGGTGCATCAACCANCTCCNGTGTAAGCNGNCTANGAAGGCTNGCACCTTTCACTTGCCCAGAGGANGCTCTGACNAAGGGGGTGCATCAACCANCTCCNGTGTAAGCNGNCTA
AGGAGTCCGAGGCAGCCCCCANCAGCTGCTGCTGTCACTGCCGCCACCTCATATTTGANAAGTCANGGTCTGCANTATGCAGGAGTCCGAGGCAGCCCCCANCAGCTGCTGCTGTCACTGCCGCCACCTCATATTTGANAAGTCANGGTCTGCANTATGC
TTGACAGTNTGCGNAAAACCTCCCATCCTTATGTANCTGACAGGNGCTTTTNCGCGNANTNACAAAAGCCACCTTGAACCCTTGACAGTNTGCGNAAAACCTCCCATCCTTATGTANCTGACAGGNGCTTTTNCGCGNANTNACAAAAGCCACCTTGAACCC
TGTCANTNCTAGGTCACCTTCNAGCTTGCCTNGACNNAAANTCNNGTCCNTTGAAACCCCCNNTGGCANGCCCAAACCCCATGTCANTNCTAGGTCACCTTCNAGCTTGCCTNGACNNAAANTCNNGTCCNTTGAAACCCCCNNTGGCANGCCCAAACCCCA
NNNTGAAGCTTNAGATNCTCAGGACNNCNNAANTTANANNNTGNCCATTTCNNACCTTANNNTTAATTTNAANNCTAAGGNCNNNTGAAGCTTNAGATNCTCAGGACNNCNNAANTTANANNNTGNCCATTTCNNACCTTANNNTTAATTTNAANNCTAAGGNC
ATTACTCTTNTTCCCCCCAAACNTNTAACNCCTTANANAGNACCNCCNNCTCTTTCCAATTNNTTNTNNCCAAATNGTNTNTNATTACTCTTNTTCCCCCCAAACNTNTAACNCCTTANANAGNACCNCCNNCTCTTTCCAATTNNTTNTNNCCAAATNGTNTNTN
NACCAGGTCCCCCAANTCTCAANNTNTAAATTTCTNNCTGNCAAACNCTTANANANTANGNTTTCCCTNNCCNAACAANTTNNACCAGGTCCCCCAANTCTCAANNTNTAAATTTCTNNCTGNCAAACNCTTANANANTANGNTTTCCCTNNCCNAACAANTTN
TGNNCANACTNTNTNCGGNGNCCC TGNNCANACTNTNTNCGGNGNCCC
BGH-Sequenz: n.d.BGH sequence: n.d.
Laufende Klon Nummer:Running clone number:
#48# 48
ACCESS No.: Selenoprotein PACCESS No .: Selenoprotein P
Definition: NM_009155.1 Gl:7110716Definition: NM_009155.1 Gl: 7110716
LOCUS: Mus musculus selenoprotein P, plasma, 1 (Seppl), mRNA complete cds.LOCUS: Mus musculus selenoprotein P, plasma, 1 (Seppl), mRNA complete cds.
Seppl 2075 bp mRNA ROD 04-JAN-2000Seppl 2075 bp mRNA ROD 04-JAN-2000
Effekt:Effect:
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-4-4
Klon #63Clone # 63
T7-Sequenz GTTTTGTTTGAAACGGCAACACTTCGGTCANGATATCTTCTACCAGACACCAAGGCGTATGGAGATAGAATANAAAGAATGT7 sequence GTTTTGTTTGAAACGGCAACACTTCGGTCANGATATCTTCTACCAGACACCAAGGCGTATGGAGATAGAATANAAAGAATG
TTCGCCTCAGNTTAAACATTGACCCTGAAGCACAGGNGGAGGAAGAACCNGAAGAAGAGCCTGAAGACACCTCAGAAGACTTCGCCTCAGNTTAAACATTGACCCTGAAGCACAGGNGGAGGAAGAACCNGAAGAAGAGCCTGAAGACACCTCAGAAGAC
GCANAAGACTCAGAGCAGGATGAGGNAGAAGAGATGGATGCAGGNACAGAANAAGAAGAGNGAGGAAACANATAAGGAAGCANAAGACTCAGAGCAGGATGAGGNAGAAGAGATGGATGCAGGNACAGAANAAGAAGAGNGAGGAAACANATAAGGAA
CTACNGANAAGGATGAATTGTAANTTATACTCTCGCTATGAATCCCGNGTGGAGAGGNAATGNGAAGTTNTGAAGTCATTTCTACNGANAAGGATGAATTGTAANTTATACTCTCGCTATGAATCCCGNGTGGAGAGGNAATGNGAAGTTNTGAAGTCATTT
TTTTGAGAGACTTGNTTTGNATGCTTCCCCNNGCCTCCTTCTCCCCTGCNCTGTNAAATGNTTGGGATTNTGGGTCACAGGTTTTGAGAGACTTGNTTTGNATGCTTCCCCNNGCCTCCTTCTCCCCTGCNCTGTNAAATGNTTGGGATTNTGGGTCACAGG
AAGAAGTGNN I I I I I IANCTGNANTNTTTTTNNCATTCCTCCTGAATGTANATTNNGTNCTATTTAACTGACTATTGGCGTCNAAGAAGTGNN I I I I IANCTGNANTNTTTTTNNCATTCCTCCTGAATGTANATTNNGTNCTATTTAACTGACTATTGGCGTCN
NAATCTTGTCNTGTGTNTNAACCCTCCCCANNCATCCCCANCTCCCCNACNTNCCCTCCNCCCCTCCNCCCTCTCTCTCNNNAATCTTGTCNTGTGTNTNAACCCTCCCCANNCATCCCCANCTCCCCNACNTNCCCTCCNCCCCTCCNCCCTCTCTCTCNN
CTCCCCTCCNNGNNCNCNCCCCNCNCATCTTCNTNNACNNGNGNCTNCCCNCCNNNTNTNCTNCCCNNTGCNCTCTCTANCTCCCCTCCNNGNNCNCNCCCCNCNCATCTTCNTNNACNNGNGNCTNCCCNCCNNNTNTNCTNCCCNNTGCNCTCTCTAN
ANNNGGGGNCCCCCTNAATTCCNTATTANCNTGNCCCCCCN ANNNGGGGNCCCCCTNAATTCCNTATTANCNTGNCCCCCCN
BGH-Sequenz: n.d.BGH sequence: n.d.
Laufende Klon Nummer: #51Running clone number: # 51
ER TransmembranproteinER transmembrane protein
ACCESS No.: J03297.1 G 193094 Definition: Mouse ERp99 mRNA encoding an endoplasmic reticulum transmembrane protein.ACCESS No .: J03297.1 G 193094 Definition: Mouse ERp99 mRNA encoding an endoplasmic reticulum transmembrane protein.
LOCUS: MUSERPX 2759 bp mRNA ROD 12-JUN-1993 3emerkung:LOCUS: MUSERPX 2759 bp mRNA ROD 12-JUN-1993 3 remark:
Effekt: Effect:
Konvention für Bewertung des phänotypischen 'Zelltod-Effektes':Convention for the evaluation of the phenotypic 'cell death effect':
- negativ, -/+ und -/++ schwach positiv, + positiv, ++ stark positiv, +++ sehr stark positiv- negative, - / + and - / ++ weakly positive, + positive, ++ strongly positive, +++ very strongly positive
Klon #65Clone # 65
Klon #66Clone # 66
Klon #67Clone # 67
Klon #68 Clone # 68
Klon #70 Clone # 70
BGH-Sequenz: TTTTTTTTTTNTNGATATTAGNTANGTTTTATTATTTNTTATCTNTATGAGGAAGGGGTATCCCAGACAGGGAGACTGNTGAG BGH sequence: TTTTTTTTTTNTNGATATTAGNTANGTTTTATTATTTNTTATCTNTATGAGGAAGGGGTATCCCAGACAGGGAGACTGNTGAG
GNNACAATCCTAGAGAGANTGTCNGAGAAGACTGANAGCGACCCTGACTCANCCGTGGTACTGGGGTGCAGTTNGTGTATGNNACAATCCTAGAGAGANTGTCNGAGAAGACTGANAGCGACCCTGACTCANCCGTGGTACTGGGGTGCAGTTNGTGTAT
GANAAGTGTGGAANAGGGAAGGGTACTGTCCCTGATACTCCCTCTGGGCTCTGCAAAAGGGAGTAGGGAGCATGAACACGGANAAGTGTGGAANAGGGAAGGGTACTGTCCCTGATACTCCCTCTGGGCTCTGCAAAAGGGAGTAGGGAGCATGAACACG
GCTGATCTGATCCTGTGNGCTANAGATGCCATGTGCGCTGCCTCTGCTCTTGATAGGCTGGNCCTCANCAGCCACAGTCAGCTGATCTGATCCTGTGNGCTANAGATGCCATGTGCGCTGCCTCTGCTCTTGATAGGCTGGNCCTCANCAGCCACAGTCA
NAANTNCGANCTCTTCACAACANCNACNCTCANACTNNAAGATCTTCCCAAAGCCTNCANGTCTTGNCTNNGNNNTGCNGANAANTNCGANCTCTTCACAACANCNACNCTCANACTNNAAGATCTTCCCAAAGCCTNCANGTCTTGNCTNNGNNNTGCNGA
TNNGAGGGACCCTGCAAGGAGCCCCTGNTCNAGGANCTGGAGGNTGTNCTNCAGGGTCNGGCCCTGGGTCTCAGGAAGTTNNGAGGGACCCTGCAAGGAGCCCCTGNTCNAGGANCTGGAGGNTGTNCTNCAGGGTCNGGCCCTGGGTCTCAGGAAGT
AGAGCGNACAGTANGCCTGCTNCAATGGNGAGGCTGCNAAAGATGACNATGGGAATCGNCTGGTGGACTGTTCTAGTAGCAGAGCGNACAGTANGCCTGCTNCAATGGNGAGGCTGCNAAAGATGACNATGGGAATCGNCTGGTGGACTGTTCTAGTAGC
ATCACAANGGGTGTGATGATGCCACCGACCCTGTAGAAGATGCTNACCAACCCCATGCCTGTTTGCCTGAτNATGGATGGTATCACAANGGGTGTGATGATGCCACCGACCCTGTAGAAGATGCTNACCAACCCCATGCCTGTTTGCCTGAτNATGGATGGT
NGANGAGCTNAGCTAGTGNACACATANGAGATGGTGAANGCCGCAACCGAGNCAANATTTCNCNATCATAGGCAGNANANNGANGAGCTNAGCTAGTGNACACATANGAGATGGTGAANGCCGCAACCGAGNCAANATTTCNCNATCATAGGCAGNANAN
AGGCNCACCGNGGGANGAACTCCTGGGATGNAAAATATAATGCCGNCCATGANCCCGNCTGGGACNGCANTCNACAGNTTAGGCNCACCGNGGGANGAACTCCTGGGATGNAAAATATAATGCCGNCCATGANCCCGNCTGGGACNGCANTCNACAGNTT
GTTTTCNNAGTTTTCNNA
Laufende Klon Nummer: UI-14 ACCESS No.: NM_004256 Definition: Homo sapiens organic cationic transporter-like 3 (ORCTL3) mRNA. LOCUS:Running clone number: UI-14 ACCESS No .: NM_004256 Definition: Homo sapiens organic cationic transporter-like 3 (ORCTL3) mRNA. LOCUS:
Effekt: -/++Effect: - / ++
Klon #72 Clone # 72
Klon #73Clone # 73
Klon #74Clone # 74
Klon #75 T7-Sequenz GCCGCCAGTGTGCTGGAAAGNGAGACTCGGCGATACTGCACTTCCTCAGAGCTTGCTGCCCACTACTGCAAGAAGTACAAClone # 75 T7 sequence GCCGCCAGTGTGCTGGAAAGNGAGACTCGGCGATACTGCACTTCCTCAGAGCTTGCTGCCCACTACTGCAAGAAGTACAA
CATCCAGTACCAGGAGAGCTTCTATGCCTGGAGCACCCCAGGCGTGGGCAAGTTNGTGACTTCCATGGCTGCCTCAGGGGCATCCAGTACCAGGAGAGCTTCTATGCCTGGAGCACCCCAGGCGTGGGCAAGTTNGTGACTTCCATGGCTGCCTCAGGGG
GCATCTATCTCACCCTGCTGTTCCTCATTGAGACCAACCTGCTGNGGCGACTGAGAACCTTCATCTGTGCCTTCCGGAGGAGCATCTATCTCACCCTGCTGTTCCTCATTGAGACCAACCTGCTGNGGCGACTGAGAACCTTCATCTGTGCCTTCCGGAGGA
GGNGGACTCTGGCAGAACTGCAGAACCGGACATCAGAGCTGCCCGAGGACCANGATGTAGCTGANGAGAGGAGCCGAATGGNGGACTCTGGCAGAACTGCAGAACCGGACATCAGAGCTGCCCGAGGACCANGATGTAGCTGANGAGAGGAGCCGAAT
CCτGGTCCCTAGCTTGGACTCCATGCTCGACACACCACTGATTATCAACGAGCTCTCCAAGGNGTATGACCAGCGAGCACCCCτGGTCCCTAGCTTGGACTCCATGCTCGACACACCACTGATTATCAACGAGCTCTCCAAGGNGTATGACCAGCGAGCACC
GCTCCTTGCCGNGGACAGGATCTCCCTTGCGGTCCAGAAAGGGGAGTGCTTCGGCCTGTTGGGTTTCAATGGAGCTGGAAGCTCCTTGCCGNGGACAGGATCTCCCTTGCGGTCCAGAAAGGGGAGTGCTTCGGCCTGTTGGGTTTCAATGGAGCTGGAA
AAACCACAACATTCAAAATGCTGACTGGGGAGGAGACCATCACCTCAGGGGACGCCTTTGTTGGTGGTTACAGCATCAGTTAAACCACAACATTCAAAATGCTGACTGGGGAGGAGACCATCACCTCAGGGGACGCCTTTGTTGGTGGTTACAGCATCAGTT
CTGACATTGGGAAGGTGCGGCAGCGGATGGGCTACTGCCCCCAGTTTGATGCACTGCTTGATCACATGACTGGCAGGGAGCTGACATTGGGAAGGTGCGGCAGCGGATGGGCTACTGCCCCCAGTTTGATGCACTGCTTGATCACATGACTGGCAGGGAG
ATGCTGGTTATGTATGCACGGCTCCGAGGCATCCCANAGCGGCTCATCAATGCCTGTGTGGAGAATACTCTGCGGGGTCTATGCTGGTTATGTATGCACGGCTCCGAGGCATCCCANAGCGGCTCATCAATGCCTGTGTGGAGAATACTCTGCGGGGTCT
GCTGCTGGAACCCGCACGCCAACAAACTAGTCAAGACTTACAGTGGTGGTAACAAACGCAGCTAAGCACTGGCATTGCCTGCTGCTGGAACCCGCACGCCAACAAACTAGTCAAGACTTACAGTGGTGGTAACAAACGCAGCTAAGCACTGGCATTGCCT
CATTGGAAAGCCTGCNGTTATCTTCTGGACATTGGAAAGCCTGCNGTTATCTTCTGGA
BGH-Sequenz: 1 1 I 1 I 1 I ICATGTCCCCTTATCCTTTATTTTTNATANAATCACAATATGTTTAAGNGTATCCTCAANANATGACACTACTCAGCBGH sequence: 1 1 I 1 I 1 I ICATGTCCCCTTATCCTTTATTTTTNATANAATCACAATATGTTTAAGNGTATCCTCAANANATGACACTACTCAGC
CCTGNANNGGTCCCTGACAATCCTGNGGCCAGNGACTTNTTGAAGGCACANAGCTAGGAACAGAGGATGNGCTTCAAGTCCCTGNANNGGTCCCTGACAATCCTGNGGCCAGNGACTTNTTGAAGGCACANAGCTAGGAACAGAGGATGNGCTTCAAGTC
TGGCCACAGCTCTCTCTTGNGCTGCCGATGCTCTGNGGGATGACATCTGGCTCAGGATCAGATCAGCCGGGTCCACTGTCTGGCCACAGCTCTCTCTTGNGCTGCCGATGCTCTGNGGGATGACATCTGGCTCAGGATCAGATCAGCCGGGTCCACTGTC
CATACTGGAAGGAACATTGACATCCAAGGATGCCTNTGCAGTTGCATTGGGAGCCAACGGTCTCAGCACCTAGTCATAGCCCATACTGGAAGGAACATTGACATCCAAGGATGCCTNTGCAGTTGCATTGGGAGCCAACGGTCTCAGCACCTAGTCATAGCC
AGTATCTTCCCTTCATGAAGGNATTTGCAACTTTGCTGCTCATTCCTGGCAGCCTGGAAGTCCTCATTGCTGCTNAACCCTGAGTATCTTCCCTTCATGAAGGNATTTGCAACTTTGCTGCTCATTCCTGGCAGCCTGGAAGTCCTCATTGCTGCTNAACCCTG
TNGGCTTTCTTCCCCCCCCCCCNAGGGGAGCTTAGNGTCATATGTAAACATAGACAGGTCCCTGGGGNCTCTCTGGATAATNGGCTTTCTTCCCCCCCCCCCNAGGGGAGCTTAGNGTCATATGTAAACATAGACAGGTCCCTGGGGNCTCTCTGGATAA
NCACTGGAAAGAAGCTCAATCCGCCAGGCTAGCTGCAGAACCAGCTGGGACAGTTCCCCATCCAAAGACTAAGGCCTGTCNCACTGGAAAGAAGCTCAATCCGCCAGGCTAGCTGCAGAACCAGCTGGGACAGTTCCCCATCCAAAGACTAAGGCCTGTC
TGTGTGCACAGCAGGATGGGGNCTGGGACTGNGGTCCAGANACCTGACTTCAGGAGTGGAAACCTGCATGCTGACTCCTTGTGTGCACAGCAGGATGGGGNCTGGGACTGNGGTCCAGANACCTGACTTCAGGAGTGGAAACCTGCATGCTGACTCCT
GCCTGCAGGCACTGACAGACACACATGGTCCACTTTGNGCATATCACCTTGANTGGTTTTCATGNCCATCATAGAAAAGAGGCCTGCAGGCACTGACAGACACACATGGTCCACTTTGNGCATATCACCTTGANTGGTTTTCATGNCCATCATAGAAAAGAG
TGATTAAAAATGANNGAAAGGAGAAAACTGGGAGGGAAAGGACCCACGGGNTAAGCNCGAAATNGCCCCTTNCACGCCCNTGATTAAAAATGANNGAAAGGAGAAAACTGGGAGGGAAAGGACCCACGGGNTAAGCNCGAAATNGCCCCTTNCACGCCCN
GGGG GGGG
Laufende Klon Nummer: UI-25 ACCESS No.: NM_001089 Definition. Homo sapiens ATP-binding cassette, sub-family A (ABC1), member 3 (ABCA3) mRNA LOCUS:Running clone number: UI-25 ACCESS No .: NM_001089 definition. Homo sapiens ATP-binding cassette, sub-family A (ABC1), member 3 (ABCA3) mRNA LOCUS:
Effekt:Effect:
Klon #76Clone # 76
T7 -Sequenz CGNCACCCCCTTGGTACCGAGCTCGGATCCCTAGTAACGGCCGCCAGTGTGCTGGAAAGGATCATCCGTCTCGCCTCCAGT7 sequence CGNCACCCCCTTGGTACCGAGCTCGGATCCCTAGTAACGGCCGCCAGTGTGCTGGAAAGGATCATCCGTCTCGCCTCCAG
TTCTTCACCGGCTGAGCTGGGTACGGACATCTCAGTGACCACGAGCTAACATAGCACGTGAAACTGACCTCAGGAAGAACTTCTTCACCGGCTGAGCTGGGTACGGACATCTCAGTGACCACGAGCTAACATAGCACGTGAAACTGACCTCAGGAAGAAC
GAAGCCTGGAGACCCGGAGTGCGACAGCGACCTCCGGACTGAAGTGGAAGAAGCGTAGCGAGGCCTTCGTGTCCTCGCTGAAGCCTGGAGACCCGGAGTGCGACAGCGACCTCCGGACTGAAGTGGAAGAAGCGTAGCGAGGCCTTCGTGTCCTCGCT
GTGGGGGGCGGGGCTTGTTGTGCAGGCGGGGCTCACGTGGTACTTTGGTACTTTGGATGACTGAAGGGAAGCTTGGTACGTGGGGGGCGGGGCTTGTTGTGCAGGCGGGGCTCACGTGGTACTTTGGTACTTTGGATGACTGAAGGGAAGCTTGGTAC
CCGCCTGGTCACTACCCGGAAGTCCAGCCGGAGTAGCACTACCCTGGAGTCCACCCGGCTGTGAAGCGGNGGTGCTCTTCCGCCTGGTCACTACCCGGAAGTCCAGCCGGAGTAGCACTACCCTGGAGTCCACCCGGCTGTGAAGCGGNGGTGCTCTT
AGGGGTGCTTGACGCCCTCAπGTTAGAAACTGATGGGTACTGTTGGCCGATTCCTAGACCCGCTTTTGACCTACGTGATAAGGGGTGCTTGACGCCCTCAπGTTAGAAACTGATGGGTACTGTTGGCCGATTCCTAGACCCGCTTTTGACCTACGTGATA
AATGTGTTTCCGGAACTCTGCCAAAAAGTGTTCTGGAGGTTTTTGTTTGTTTCGGAGCCAAGGCCTCCCTCGTAGCTCTTAAAATGTGTTTCCGGAACTCTGCCAAAAAGTGTTCTGGAGGTTTTTGTTTGTTTCGGAGCCAAGGCCTCCCTCGTAGCTCTTAA
TGGCCTGAAACACTCTTTGTAGCCTAGACTGGCATTGAAGTCAAGTTAGCCCTGCCTTTGCCTCCCTGGGCTCTGGGATTATGGCCTGAAACACTCTTTGTAGCCTAGACTGGCATTGAAGTCAAGTTAGCCCTGCCTTTGCCTCCCTGGGCTCTGGGATTA
CCCTGCCTTTCTTGATTATTTCTATTTGAACAAATATGAACAAACTATGTGGCCACCACAAACCCTGCCTTTCTTGATTATTTCTATTTGAACAAATATGAACAAACTATGTGGCCACCACAAA
BGH-Sequenz: TT I I I I I l I I l IGTGGNGGCCACATAGTTTGTTCATATTTGTTCAAATAGAAATAATCAAGAAAGGCAGGGTAATCCCAGAGCBGH sequence: TT I I I I I l I I l IGTGGNGGCCACATAGTTTGTTCATATTTGTTCAAATAGAAATAATCAAGAAAGGCAGGGTAATCCCAGAGC
CCANGGAGGCAAAGGCAGGGCTAACTTGACTTCAATGCCAGTCTAGGCTACAAAGAGTGTTTNAGGCCATTAAGAGCTACGCCANGGAGGCAAAGGCAGGGCTAACTTGACTTCAATGCCAGTCTAGGCTACAAAGAGTGTTTNAGGCCATTAAGAGCTACG
AGGGAGGCCTTGGCTCCGAMCAMCAAAAACCTTCAGAACACTTTTTGGCAGAGTTCCGGAAACACATTTATCACGTAGGAGGGAGGCCTTGGCTCCGAMCAMCAAAAACCTTCAGAACACTTTTTGGCAGAGTTCCGGAAACACATTTATCACGTAGG
TCAAAAGCGGGTCTAGGAATCGGCCAACAGTACCCATCAGTTTCTAACAATGAGGGCGTNAAGCACCCCTAAGAGCACCACTCAAAAGCGGGTCTAGGAATCGGCCAACAGTACCCATCAGTTTCTAACAATGAGGGCGTNAAGCACCCCTAAGAGCACCAC
CGCTTCACAGCCGGGTGGACTCCAGGGTAGTGCTACTCCGGCTGGACTTCCGGGTAGTGACCAGGCGGGTACCAAGCTTCGCTTCACAGCCGGGTGGACTCCAGGGTAGTGCTACTCCGGCTGGACTTCCGGGTAGTGACCAGGCGGGTACCAAGCTT
CCCTTCAGTCATCCAAAGTACCAAAGTACCACGTGAGCCCCGCCTGCACAACAAGCCCCGCCCCCCACAGCGAGGACACGCCCTTCAGTCATCCAAAGTACCAAAGTACCACGTGAGCCCCGCCTGCACAACAAGCCCCGCCCCCCACAGCGAGGACACG
AAGGCCTCGCTACGCTTCTTCCACTTCAGTCCGGAGGTCGCTGTCGCACTCCGGGTCTCCAGGCTTCGTTCTTCCTGAGGAAGGCCTCGCTACGCTTCTTCCACTTCAGTCCGGAGGTCGCTGTCGCACTCCGGGTCTCCAGGCTTCGTTCTTCCTGAGG
TCAGTTTCACGTGCTATGTTAGCTCGNGGNCACTGAGAT TCAGTTTCACGTGCTATGTTAGCTCGNGGNCACTGAGAT
Laufende Klon Nummer: UI-26Current clone number: UI-26
ACCESS No.: ESTs AI315969, AI930239, AI666299, AV141103ACCESS No .: ESTs AI315969, AI930239, AI666299, AV141103
Definition: mouse ESTsDefinition: mouse ESTs
LOCUS:LOCUS:
Effekt: -/+Effect: - / +
Klon #77Clone # 77
T7-Sequenz TTCACCCCGCTTGGTACCGAGCTCGGATCCCTAGTAACGGCCGCCAGTGTGCTGGAAAGATGGAGCCTGCGTTTCACAGAT7 sequence TTCACCCCGCTTGGTACCGAGCTCGGATCCCTAGTAACGGCCGCCAGTGTGCTGGAAAGATGGAGCCTGCGTTTCACAGA
GGGGATCTCCTTTTCCTCACGAACCGAGTTGAAGATCCTATACGCGTGGGGGAGATCGTTGTTTTCAGGATAGAAGGAAGAGGGGATCTCCTTTTCCTCACGAACCGAGTTGAAGATCCTATACGCGTGGGGGAGATCGTTGTTTTCAGGATAGAAGGAAGA
GAGATTCCTATAGTGCACCGAGTCCTGAAGATCCATGAAAAGCAAGATGGGCATATCAAGTTTTTAACCAAAGGAGATAATAGAGATTCCTATAGTGCACCGAGTCCTGAAGATCCATGAAAAGCAAGATGGGCATATCAAGTTTTTAACCAAAGGAGATAATA
ATGCTGTTGATGACCGAGGTCTCTATAAACAAGGACAACACTGGCTGGAGAAGAAAGATGTTGTGGGGAGAGCAAGAGGGATGCTGTTGATGACCGAGGTCTCTATAAACAAGGACAACACTGGCTGGAGAAGAAAGATGTTGTGGGGAGAGCAAGAGGG
TTTGTTCCTTACATTGGAATTGTGACGATCCTCATGAATGACTATCCTAAATTT GTATGCAGTACTGTTTCTGCTCGGTTTTTTGTTCCTTACATTGGAATTGTGACGATCCTCATGAATGACTATCCTAAATTT GTATGCAGTACTGTTTCTGCTCGGTTT
ATΓTGTGCTGGTCCATCGTGAGTAAGAAGTCGGACTCCCTGTTCCTAGGAAGCTGCTGTGCTTGTTGTTACTGAATGTTGGATΓTGTGCTGGTCCATCGTGAGTAAGAAGTCGGACTCCCTGTTCCTAGGAAGCTGCTGTGCTTGTTGTTACTGAATGTTGG
AGTAGAΤCCTGATCTGTGATΤGCGGATTTTCGGAGGACACACACGTTGGCACTTCTTGGTAGCCCTGGTTTGCATTGCTTTAGTAGAΤCCTGATCTGTGATΤGCGGATTTTCGGAGGACACACACGTTGGCACTTCTTGGTAGCCCTGGTTTGCATTGCTTT
GTGTTTCCACACCAGAGGCTGTGTGGGCGGGTGCATGTGCACCGTGGAGTGCACACAAGGGGACTGTCAATCACAGGGTGTGTTTCCACACCAGAGGCTGTGTGGGCGGGTGCATGTGCACCGTGGAGTGCACACAAGGGGACTGTCAATCACAGGGT
TTCATATGTTGTCATTGTCACTCTTTCACATTTTTGTCATCAGTGMTTTTTTATATTAA GGTTGAGCCAA^TTCATATGTTGTCATTGTCACTCTTTCACATTTTTGTCATCAGTGMTTTTTTATATTAA GGTTGAGCCAA ^
TTTGTATTTTGAAGCCNAGCTTCACTTTAA GTGCCTACAGAGTTCTGTAAATGAAAACACAGCTCTGCATGAGTTCAAACCTTTGTATTTTGAAGCCNAGCTTCACTTTAA GTGCCTACAGAGTTCTGTAAATGAAAACACAGCTCTGCATGAGTTCAAACC
TGNCGGTCCTTCTTACAGTAGGAATGGCNCATANTGAGGCGGGCATAAGTCTTACTTT TGNCGGTCCTTCTTACAGTAGGAATGGCNCATANTGAGGCGGGCATAAGTCTTACTTT
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Klon #79 Clone # 79
Klon #80Clone # 80
-4-4
Klon #81Clone # 81
Klon #82Clone # 82
Klon #83Clone # 83
Klon #84 T7-Sequenz TNTGACGCCGTTCTAGCATTNAGGTGACACTATAG TAGGGCCCTCTAGATGCATGCTCGAGCGGCCGCCCTTTTTTTTClone # 84 T7 sequence TNTGACGCCGTTCTAGCATTNAGGTGACACTATAG TAGGGCCCTCTAGATGCATGCTCGAGCGGCCGCCCTTTTTTTT
I I I I I I I I I I CCGTTATAATAGCCATCTTTATTTGTAAAAATCCAGATATAAAACGTAATCTTTCAGTCTTTCCAGGTTTTCCTI I I I I I I I I CCGTTATAATAGCCATCTTTATTTGTAAAAATCCAGATATAAAACGTAATCTTTCAGTCTTTCCAGGTTTTCCT
TTTTACAAAAACAAAAAGGCACGTATAAACCTTGCCCGCTGTCGTCCCCGTACACGGNGTTTCTCAGGCAGCCCTCCCCCTTTTACAAAAACAAAAAGGCACGTATAAACCTTGCCCGCTGTCGTCCCCGTACACGGNGTTTCTCAGGCAGCCCTCCCCC
CGCCCCGCCCCCCGTTACAGCTACATGCTTCATTCCAGGACGTCTGCATCCCCACATGCTTTGGNGCTTTCCTACCAGGCGCCCCGCCCCCCGTTACAGCTACATGCTTCATTCCAGGACGTCTGCATCCCCACATGCTTTGGNGCTTTCCTACCAGG
AGAGTTCCGAGCTCCAAGACTTGAAGTACACAAAGAGGGGGTAGGGGTGGGTGCAGNGTGTGGCACAATGTTCCACGGAGAGTTCCGAGCTCCAAGACTTGAAGTACACAAAGAGGGGGTAGGGGTGGGTGCAGNGTGTGGCACAATGTTCCACGG
GTGCAGGGCAGNGGGCTAGTAGTAGGTCTCCTTCTCCACCCAGCCGCCAGGGCGCCGCCTGATAATGAGCTTCCGCACGTGCAGGGCAGNGGGCTAGTAGTAGGTCTCCTTCTCCACCCAGCCGCCAGGGCGCCGCCTGATAATGAGCTTCCGCAC
TCGTCATACACAAAGATGAGAAGGGAGTAGGGGAAGGCACAGAACCACCATGTAGGTTTGAGGGGATACATCCTAAAGGTCGTCATACACAAAGATGAGAAGGGAGTAGGGGAAGGCACAGAACCACCATGTAGGTTTGAGGGGATACATCCTAAAGG
TGCCCCCATCCCGGGGCAGTAGGATAAGAAAGCAGCAAGGGCTGTCTCTTCAAAGAGGCCAAATATCAAGATCTTGGTCTGCCCCCATCCCGGGGCAGTAGGATAAGAAAGCAGCAAGGGCTGTCTCTTCAAAGAGGCCAAATATCAAGATCTTGGTC
CATTCCCTGCTGGACATTCCCTGCTGGA
BGH-Sequenz: TTCCANCCCCCCTTGGACCGAGCTCGGATCCTCTAGTAACGTCCGCCAGTGTGCTGGAAAGGGGTTGCCATGGGGATTGBGH sequence: TTCCANCCCCCCTTGGACCGAGCTCGGATCCTCTAGTAACGTCCGCCAGTGTGCTGGAAAGGGGTTGCCATGGGGATTG
TGGCTCTGATGTGTCCAAGCAAGCTGCTGACATGATCCTTCTGGATGACAACTTTGCCTCCATTGTGACTGGAGTAGAGGTGGCTCTGATGTGTCCAAGCAAGCTGCTGACATGATCCTTCTGGATGACAACTTTGCCTCCATTGTGACTGGAGTAGAGG
AGGTCGTCTGATTTTTGATAACTTGAAGAAATCCATCGCTTACACCCTAACCAGTAACATTCCGGAAATCACCCCCTTCTTAGGTCGTCTGATTTTTGATAACTTGAAGAAATCCATCGCTTACACCCTAACCAGTAACATTCCGGAAATCACCCCCTTCTT
TATTTATTATTGCAAACATTCCACTGCCCCTGGGGACTGTGACCATCCTCTGCATTGACTTGGGCACTGACATGGTTCCTGTATTTATTATTGCAAACATTCCACTGCCCCTGGGGACTGTGACCATCCTCTGCATTGACTTGGGCACTGACATGGTTCCTG
CATCTCCCTGGCCTACGAGCAAGCTGAGAGCGACATCATGAAGAGGCAGCCCAGAAACCCCAAAACGGACAAACTTGTGCATCTCCCTGGCCTACGAGCAAGCTGAGAGCGACATCATGAAGAGGCAGCCCAGAAACCCCAAAACGGACAAACTTGTG
ACGAGCGTCTGATCAGCATGGCCTATGGACAGATCGGTATGATCCAGGCCCTGGGAGGCTTCTTCACTTACTTTGNGATTACGAGCGTCTGATCAGCATGGCCTATGGACAGATCGGTATGATCCAGGCCCTGGGAGGCTTCTTCACTTACTTTGNGATT
TGGCTGAGAACGGTTTCCTGCCCTTTCACCTGTTGGGCATCCGAGAGACCTGGGATGACCGCTGGGTCAACGATGTGGATGGCTGAGAACGGTTTCCTGCCCTTTCACCTGTTGGGCATCCGAGAGACCTGGGATGACCGCTGGGTCAACGATGTGGA
GACAGCTACGGGCAGCAGTGGACCTACGAGCAGAGGAAGATCGTGGAGTTCACCTGCCATACAGCGTTCTTTGTCAGTAGACAGCTACGGGCAGCAGTGGACCTACGAGCAGAGGAAGATCGTGGAGTTCACCTGCCATACAGCGTTCTTTGTCAGTA
TGTGGTAGNGCAGTGGGCCGACTTGGTCATCTGC TGTGGTAGNGCAGTGGGCCGACTTGGTCATCTGC
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3V9N999W39V3V99V39NV9VN9V9V999 99913J.1V1311V319199V19V9131911V3931V3911VVV99V91VVVV1VV133111331913V33313V339199VV 991333V9JJL331133919333331333391119JL19131V339V339119V13113391913V931339V31V91393133V9N999W39V3V99V39NV9VN9V9V999 99913J.1V1311V319199V19V9131911V3931V3911VVV99V91VVVV1VV133111331913V33313V339199VV 991333V9JJL33113391911991391399933913913933913913913933913913913933913913913913913913913913913913913913913913913913913913913933913913913913923
9V9V1391VW133V31919V1V1311V1333999V9V131V391V39V9313933993999W^^ VV3JL1V9VVV1V3V91VV13VVV1VW1911VVVVVV9V13V1V11313W 1119VW9W91W9V3W.LU3331V311W1.UV1V99 9.Lα999131V991113V191933V91V91V1399139119939919\Λ 3VVVV9919V3 l I I IV3391HW9W999939V9V1391VW133V31919V1V1311V1333999V9V131V391V39V9313933993999W ^^ VV3JL1V9VVV1V3V91VV13VVV1VW1911VVVVVV9V13V1V11313W 1119VW9W91W9V3W.LU3331V311W1.UV1V99 9.Lα999131V991113V191933V91V91V1399139119939919 \ Λ 3VVVV9919V3 l I I IV3391HW9W99993
31999V99V91V13V339V39319331991933133V93V31199V99331V19V9V339991191V3V939V39V331 13W9V3931333993913199VW991391919V33933993W19V11331V993139V933V199JJL3N333N9311 zuanbag-z31999V99V91V13V339V39319331991933133V93V31199V99331V19V9V339991191V3V939V39V331 13W9V3931333993913199VW991391919V33933993W19V11331V993139V933V199J93L933V199JJ
98# uo|98 # uo |
BGH-Sequenz: I I I I I I I I I I I I I I 1 I I I I I GAATCTTTATGTCATTAGTTTATTTACAATTTTTTCTAGTATAAGAGTTCAAGAGTTTAATCCAATBGH sequence: I I I I I I I I I I I I I I 1 I I I I I GAATCTTTATGTCATTAGTTTATTTACAATTTTTTCTAGTATAAGAGTTCAAGAGTTTAATCCAAT
TTCAGATCACCTCTTA CTTTCTTCATTCTGTTAAAGGGATGAATTAAATATCCTTATTTTTTAAGTAGCTGGTGTCTTACTATTCAGATCACCTCTTA CTTTCTTCATTCTGTTAAAGGGATGAATTAAATATCCTTATTTTTTAAGTAGCTGGTGTCTTACTA
A GAAAGGTGCGGCA CCCAGATCCAMGTACACGGTCATCATAGCCAGCAACCGCCACTTGTTTTCCACTGAAAATGGA GAAAGGTGCGGCA CCCAGATCCAMGTACACGGTCATCATAGCCAGCAACCGCCACTTGTTTTCCACTGAAAATGG
CAAATTCTTCCCCGGACCCTCCTCATAGNGGCTGCGACGGACCACGGAGGTCGTGAACCTCCGGATACTCTGGCCCAACACAAATTCTTCCCCGGACCCTCCTCATAGNGGCTGCGACGGACCACGGAGGTCGTGAACCTCCGGATACTCTGGCCCAACA
TGTCGCTGCTGGAAGTTCTGCGAGGGCCGCAGACCTTTCCAGCACACTGGCGGCCGTTACTAGTGGATCCGAGCTCGGTTGTCGCTGCTGGAAGTTCTGCGAGGGCCGCAGACCTTTCCAGCACACTGGCGGCCGTTACTAGTGGATCCGAGCTCGGT
CCAAGCTTGGGTCTCCCTATAGNGAGTCGTATTAATTTCGATAAGCCAGTAAGCAGNGGGTTCTCTAGTTAGCCAGAGAGCCCAAGCTTGGGTCTCCCTATAGNGAGTCGTATTAATTTCGATAAGCCAGTAAGCAGNGGGTTCTCTAGTTAGCCAGAGAGC
TCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTC TGGGGCGGAGTTGTTACGACATTTTGGAAATCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTC TGGGGCGGAGTTGTTACGACATTTTGGAAA
GTCCCGTTGATTGTCCCGTTGATT
Laufende Klon Nummer: UI-61 ACCESS No.: NM_007749 Definition: Mus musculus cytochrome c oxidase subunit Vllc (Cox7c), mRNA LOCUS:Current clone number: UI-61 ACCESS No .: NM_007749 Definition: Mus musculus cytochrome c oxidase subunit Vllc (Cox7c), mRNA LOCUS:
Effekt: -/++ (moderat schnell)Effect: - / ++ (moderately fast)
Klon #86 Clone # 86
4-4
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CVlCVI
CVlCVI
Strand- Plus /PlusBeach Plus / Plus
Klon #122 T7-Sequenz ATGCACCACCAGTCTGTTCTGCACAGCGGCTACTTTCACCCACTGCTTCGGAGCTGGCAGACTGCTGCCTCCACCGTCA Clone # 122 T7 sequence ATGCACCACCAGTCTGTTCTGCACAGCGGCTACTTTCACCCACTGCTTCGGAGCTGGCAGACTGCTGCCTCCACCGTCA
Tabelle 2Table 2
Klon 0001-T7-SequenzClone 0001-T7 sequence
AGCTCATGTTGGACAGTCGTGTGAGGAGCTATGGAGCACAGCAGTAATCGCCCAGAGGACTTCCCGCTTAACGTGTTCTC TGTCACTCCGTACACACCCAGTACCGCCGACATCCAGGTGTCCGACGACGACAÄGGCAGGGGCCACTTTGCTΓTTCTCAG GCATCTTTCTAGGACTGGTGGGGATCACTTTCACTGTCATGGGCTGGATCAAATACCÄAGGTGTCTCCCACTTTGTANAT GGACCCAGCTCCTCGGACCCATCCTTCTGTCGGTCGGAGTGACATTCATCCTGATCGCTGTGTGCAAATTCAAAAYGCTA TCCTGCCAGTTGTGCTCAGATAACGAGGAGAGGGTCCCGGGACTCCGGACCAGGACTTCCSGAGGACAGTCGTTCGTTTT CACTGGCATCAATCAGCCCATCACCTTCCACGGGGCCACCGTGGTGCAGTATATCCCTCCTCCTTACGGTTCTCAGGAGC CCCTGGGAATGAACGCCACCTACCTGCAACCCATGATGAATCCTTGCGGTCTCATACCTCCTAGTGGAGCAGCGGCTGCC GCACCAAGTCCCCCTCAGTACTACACCATCTACCCTCAAGACAATGCTGCGTTCGTNGAGAGNTGAGGGCTTCTCTCCTT TCGTGGGCACTGGATATGACAGGCCCGACTCTGATGCTGACCAGCTAGAAGGGACGGAGTTGGAAGAGGAGGACTGCGTA TGTTTCTCTYCTCCACCGTATGAGGAGATATACGCTCTACCTCGCTAGAGACTGCAATGCTAAGGGGACGGACATTTAAG CCCCTGNGATGTGATACTTGGAGAGTTTATCGCTGAGTTCTTCAGAAGTTAGGTGTCAAAGCAGCTNAGGAGATCTTACA GATGTCATTNAAGGNGGGAAAGAAGTGCCCNGAGACTGCTAAATTAAGCTGCCCTGGTTAAATTCCCCTCTGCTCTGGTT TTGAATTCTCTCAGCTAAGAAACCCTCTGCAGCTGGAGAGTCGCTCTGAGATAGAGAGATTTNGGAGCCCACGCAGNGCCAGCTCATGTTGGACAGTCGTGTGAGGAGCTATGGAGCACAGCAGTAATCGCCCAGAGGACTTCCCGCTTAACGTGTTCTC TGTCACTCCGTACACACCCAGTACCGCCGACATCCAGGTGTCCGACGACGACAÄGGCAGGGGCCACTTTGCTΓTTCTCAG GCATCTTTCTAGGACTGGTGGGGATCACTTTCACTGTCATGGGCTGGATCAAATACCÄAGGTGTCTCCCACTTTGTANAT GGACCCAGCTCCTCGGACCCATCCTTCTGTCGGTCGGAGTGACATTCATCCTGATCGCTGTGTGCAAATTCAAAAYGCTA TCCTGCCAGTTGTGCTCAGATAACGAGGAGAGGGTCCCGGGACTCCGGACCAGGACTTCCSGAGGACAGTCGTTCGTTTT CACTGGCATCAATCAGCCCATCACCTTCCACGGGGCCACCGTGGTGCAGTATATCCCTCCTCCTTACGGTTCTCAGGAGC CCCTGGGAATGAACGCCACCTACCTGCAACCCATGATGAATCCTTGCGGTCTCATACCTCCTAGTGGAGCAGCGGCTGCC GCACCAAGTCCCCCTCAGTACTACACCATCTACCCTCAAGACAATGCTGCGTTCGTNGAGAGNTGAGGGCTTCTCTCCTT TCGTGGGCACTGGATATGACAGGCCCGACTCTGATGCTGACCAGCTAGAAGGGACGGAGTTGGAAGAGGAGGACTGCGTA TGTTTCTCTYCTCCACCGTATGAGGAGATATACGCTCTACCTCGCTAGAGACTGCAATGCTAAGGGGACGGACATTTAAG CCCCTGNGATGTGATACTTGGAGAGTTTATCGCTGAGTTCTTCAGAAGTTAGGTGTCAAAGCAGCTNAGGAGATCTTACA GATGTCATTNAAGGNGGGAAAGAAGTGCCCNGAGACTGCTAAATTAAGCTGCCCTGGTTAAATTCCCCTCTGCTCTGGTT TTGAATTCTCTCAGCTAAGAAACCC TCTGCAGCTGGAGAGTCGCTCTGAGATAGAGAGATTTNGGAGCCCACGCAGNGCC
TT
Klon 0001-BGH-SequenzClone 0001 BGH sequence
ATAGACATGTCTAGTTTTTTATTACTAGTTATCATCCAAGTGAAATGTCCCTGAGGCATAATATGAATCACAATAATAAAATAGACATGTCTAGTTTTTTATTACTAGTTATCATCCAAGTGAAATGTCCCTGAGGCATAATATGAATCACAATAATAAA
CCAAGATTGTTTTGCTGATATCCTTGAAAAACCTGGACTCTTCTGACAGAGAGTAAAAGCAATCCCACATATAAGCACAGCCAAGATTGTTTTGCTGATATCCTTGAAAAACCTGGACTCTTCTGACAGAGAGTAAAAGCAATCCCACATATAAGCACAG
TACCAAAACCTTCAAGACCTGACAATTCCTGTATCTCTCTTGGGGGCAGCCACCTTTACCTTGAGTGGCCTTTGATCTTTTACCAAAACCTTCAAGACCTGACAATTCCTGTATCTCTCTTGGGGGCAGCCACCTTTACCTTGAGTGGCCTTTGATCTTT
GTAAAACTGTTTTCTTTCTAATTCTTTGGACACCTCCTAAAGTCTGCAGCTTTGGGCTGNGGTTGGTGACACCGTTCAGAGTAAAACTGTTTTCTTTCTAATTCTTTGGACACCTCCTAAAGTCTGCAGCTTTGGGCTGNGGTTGGTGACACCGTTCAGA
CTTAGGAAGCTGAAGGCTACCAGCTTTTCTTTGTTCAAATGAAGGTGÄGAGC ACGCCCGGGGGCGGAGGKAGRGGGAGACTTAGGAAGCTGAAGGCTACCAGCTTTTCTTTGTTCAAATGAAGGTGÄGAGC ACGCCCGGGGGCGGAGGKAGRGGGAGA
CGGGATGACTGATCATCTCCATGATTCATGACTACAAAGCGACACGGCAGNATGGCTCTGNGGAACAGATCAGAGGCATACGGGATGACTGATCATCTCCATGATTCATGACTACAAAGCGACACGGCAGNATGGCTCTGNGGAACAGATCAGAGGCATA
CCTGTGTTCCATAAGCNACTCAGCTGTACCTGCGTTCTGTAAGCCACTCGGCTGAGCTAGTTCGNGCCTGGGAATCACAGCCTGTGTTCCATAAGCNACTCAGCTGTACCTGCGTTCTGTAAGCCACTCGGCTGAGCTAGTTCGNGCCTGGGAATCACAG
CCTGGGGGNGGGCAGAGGGAGCAGGCGCTNACCNATGTACATTACGCCTTGAACTTCANCCTTGCAAAGGAAAACNATCA NGAGCAGGGGTCACTNTAACTCGGNGGACAGCACATGGNGACATTCATTATCCTCCTGNTACCTCGTCCACAGTAGGATC GGCCACCCAACACTATTCTAACATGGGGCACTGTTTCATCCNACTCTACTTATTCATTTATTTCTTAATGGATTTTATTT CTTTAACAAACTCTTNAAGATCCAAAGGCTTCCAGNGAGACCANATAANAGTCNAAGTTGTCTAANATAAGTNACTCTGC TGCGGAAGGTTCAGNGCCGTGCAGGGAAATTTCTACCTGAGCCTGCTCTCTTCCCTGCTTGCTTGCCAGCCTCCCTTTNA TCANTCTNAGAGCTGCCATGGCTGCCCGGATTTAAΆACTAΆNACAATCTTTNAAAACTAAGATNTCCTTTAAATGGATAT TCCGCATGGCCTGGGGGNGGGCAGAGGGAGCAGGCGCTNACCNATGTACATTACGCCTTGAACTTCANCCTTGCAAAGGAAAACNATCA NGAGCAGGGGTCACTNTAACTCGGNGGACAGCACATGGNGACATTCATTATCCTCCTGNTACCTCGTCCACAGTAGGATC GGCCACCCAACACTATTCTAACATGGGGCACTGTTTCATCCNACTCTACTTATTCATTTATTTCTTAATGGATTTTATTT CTTTAACAAACTCTTNAAGATCCAAAGGCTTCCAGNGAGACCANATAANAGTCNAAGTTGTCTAANATAAGTNACTCTGC TGCGGAAGGTTCAGNGCCGTGCAGGGAAATTTCTACCTGAGCCTGCTCTCTTCCCTGCTTGCTTGCCAGCCTCCCTTTNA TCANTCTNAGAGCTGCCATGGCTGCCCGGATTTAAΆACTAΆNACAATCTTTNAAAACTAAGATNTCCTTTAAATGGATAT TCCGCATGG
Klon 0002-ContigClone 0002 contig
CGCCGCCTGAGCGCCCGGCCCGACCCCGCCATGGGGTGCTGCTATAGCAGCGAAAACGAGGACTCGGACCAGGATCGGGACGCCGCCTGAGCGCCCGGCCCGACCCCGCCATGGGGTGCTGCTATAGCAGCGAAAACGAGGACTCGGACCAGGATCGGGA
GGAGAGGAAGCTGCTGCTGGACCCCAGTAGCACCCCTACCAAAGCCCTCAATGGAGCCGAGCCCAACTACCATAGCCTACGGAGAGGAAGCTGCTGCTGGACCCCAGTAGCACCCCTACCAAAGCCCTCAATGGAGCCGAGCCCAACTACCATAGCCTAC
CTTCAGCTCGCACAGATGAGCAGGCCCTGCTTTCCTCCATCCTTGCCAAGACAGCTAGCAACATCATTGATGTGTCTGCCCTTCAGCTCGCACAGATGAGCAGGCCCTGCTTTCCTCCATCCTTGCCAAGACAGCTAGCAACATCATTGATGTGTCTGCC
GCAGACTCCCAGGGCATGGAACAGCATGAGTACATGGACCGGGCAAGGCAGTACAGTACCCGCTTGGCTGTGCTTAGCAGGCAGACTCCCAGGGCATGGAACAGCATGAGTACATGGACCGGGCAAGGCAGTACAGTACCCGCTTGGCTGTGCTTAGCAG
CAGTCTGACCCATTGGAAGAAGCTGCCACCGTTGCCATCTCTCACCAGCCAGCCCCÄCCAAGTGCTGGCCAGTGAGCCTACAGTCTGACCCATTGGAAGAAGCTGCCACCGTTGCCATCTCTCACCAGCCAGCCCCÄCCAAGTGCTGGCCAGTGAGCCTA
TCCCCTTCTCTGACTTGCAGCAGGTCTCCAGGATAGCTGCGTATGCCTATAGTGCACTTTCTCAGATCCGCGTGGATGCG AAAGAAGAGCTGGTTGTACAGTTTGGGATCCCATGAAGAGAGGGGCCCTAGGACAGCTCTTCCCTCGTCTTCACCCCGTC TCCACCCCACCTCTTCTGGCCCCCAGCCTCACTGTGGCTCTCTACAGTACCTAACCTGCTACTAΆTCACGGAGAAGAATG TGGAGGGAAAGAACAAGGCTGGAGGCCGGAGCAAGTGAGGACTAAGCAAGGGAAGGGAGGACCGATTGCCATCGGCCTTC ATGCTCTGGTTAGGGTGAGGTTGGGGCCAAGAGGACAGGGCCTGGCAGATCTTCAGTCATTGGGAAGATGGAGATACCNC TGTAGGGGTGACACCGGGAGACCTAGGAGATCCCTTCCTGCCCTCTTTCTCTTGGCCTCCGATTCACTCCTGTCCCCTTC CCTGACTTGGTGCTCACAGGCACCTCACTGGGGATTATGACCAGGGTCTAGACGAGCTTGAGTCTGAATTGAGTTTGTAT TTCTAGCACCCTGGGTTTTTACATGTTTGCTCTTTTTGTTTTTGTTTGTCACCCCTCGATAAAGAAAGTATATTCATTTG AAAAAAAAAAAAAAAAAAATCCCCTTCTCTGACTTGCAGCAGGTCTCCAGGATAGCTGCGTATGCCTATAGTGCACTTTCTCAGATCCGCGTGGATGCG AAAGAAGAGCTGGTTGTACAGTTTGGGATCCCATGAAGAGAGGGGCCCTAGGACAGCTCTTCCCTCGTCTTCACCCCGTC TCCACCCCACCTCTTCTGGCCCCCAGCCTCACTGTGGCTCTCTACAGTACCTAACCTGCTACTAΆTCACGGAGAAGAATG TGGAGGGAAAGAACAAGGCTGGAGGCCGGAGCAAGTGAGGACTAAGCAAGGGAAGGGAGGACCGATTGCCATCGGCCTTC ATGCTCTGGTTAGGGTGAGGTTGGGGCCAAGAGGACAGGGCCTGGCAGATCTTCAGTCATTGGGAAGATGGAGATACCNC TGTAGGGGTGACACCGGGAGACCTAGGAGATCCCTTCCTGCCCTCTTTCTCTTGGCCTCCGATTCACTCCTGTCCCCTTC CCTGACTTGGTGCTCACAGGCACCTCACTGGGGATTATGACCAGGGTCTAGACGAGCTTGAGTCTGAATTGAGTTTGTAT TTCTAGCACCCTGGGTTTTTACATGTTTGCTCTTTTTGTTTTTGTTTGTCACCCCTCGATAAAGAAAGTATATTCATTTG ÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁ
Klon 0003-T7-SequenzClone 0003-T7 sequence
AGGTTCCTGTCGTTTATGCTGCGCGCCGCGAGCATCTTCGTGAAGGACGCTGTGCTCTASTCTGGCTTCACGCTGGATGA GGCCGAGCGCCTCACGGAGGAAGAGTTGCATATCATCGCGCAGGTGCCGCCTCCACCCGCCGCTGCCGCCGTTGGCTACG CTGGCTGCCGTGTGGCTGTGACCTTCTTCCTCTACTTCCTGGCTACCAACTACTACTGGATTCTGGTGGAGGGACTGTAC TTACACAGCCTCATCTTCATGGCCTTTTTCTCAGAGAAGAAGTATCTGTGGGGCTTCACCAΓCTTTGGCTGGGGTCTGCC GGCTGTCTTCGTGGCTGTGTGGGTCGGTGTCAGAGCAACCTTGGCCAACACTGGGTGCTGGGACCTGAGCTCTGGGCACA AGAAGTGGATCATCCAGGTGCCCATCCTGGCATCTGTTGTGCTCAACTTCATCCTCTTTATCAACATCATCCGGGTGCTT GCCACTAAGCTTCGGGAGAC AATGCGGGCCGGTGTGACACCAGGCAGYAGTACCGGAAGCTGCTCACGTCCACGTTGGT GCYNGCGCCACTCTTTKGTGTCCACTACACCCGTCTTCATGGCCTTGCCGTACACCCGAGGTCTTAGGGACACTGGGGCA GATCCAGATGCACTATGAGATGCTCTTTAACTCCTTTCAGGAGGTTCCTGTCGTTTATGCTGCGCGCCGCGAGCATCTTCGTGAAGGACGCTGTGCTCTASTCTGGCTTCACGCTGGATGA GGCCGAGCGCCTCACGGAGGAAGAGTTGCATATCATCGCGCAGGTGCCGCCTCCACCCGCCGCTGCCGCCGTTGGCTACG CTGGCTGCCGTGTGGCTGTGACCTTCTTCCTCTACTTCCTGGCTACCAACTACTACTGGATTCTGGTGGAGGGACTGTAC TTACACAGCCTCATCTTCATGGCCTTTTTCTCAGAGAAGAAGTATCTGTGGGGCTTCACCAΓCTTTGGCTGGGGTCTGCC GGCTGTCTTCGTGGCTGTGTGGGTCGGTGTCAGAGCAACCTTGGCCAACACTGGGTGCTGGGACCTGAGCTCTGGGCACA AGAAGTGGATCATCCAGGTGCCCATCCTGGCATCTGTTGTGCTCAACTTCATCCTCTTTATCAACATCATCCGGGTGCTT GCCACTAAGCTTCGGGAGAC AATGCGGGCCGGTGTGACACCAGGCAGYAGTACCGGAAGCTGCTCACGTCCACGTTGGT GCYNGCGCCACTCTTTKGTGTCCACTACACCCGTCTTCATGGCCTTGCCGTACACCCGAGGTCTTAGGGACACTGGGGCA GATCCAGATGCACTATGAGATGCTCTTTAACTCCTTTCAGG
Klon 0004-ContigClone 0004 contig
AGGCGCTGCCTACCAGAGCGCAGCATGACGGCCΆTCGGCGCGCAGGCCCACAAGCTGTTGGGCCTTAAGAGGCCCCACCG GTCTTTCTTTGAGTCCTTCATCCGGACACTCATCATCGTGTGCACTGCCCTGGCTGTGGTCCTTTCTTCAGTCTCCATCT GCGATGGCCACTGGCTCCTAGTGGAGGATCATCTCTTTGGGCTGTGGTACTTCTGCACCATCGGCAACCACAGTGAACCA CACTGTCTGAGAGACCTGAGCCAGGCCCATATGCCCGGGCTGGCTGTAGGCATGGGCCTAGCACGCAGTGTGGCCGCCAT GGCAGTGGTGGCTGCCATCTTCGGCTTGGAGATGCTCATTGTGTCCCAGGTGTGTGAAGATGTCCGCTCACGGCGCAÄGT GGGCCATCGGTTCCTACCTCCTCCTGGTTGCCTTTATCCTCTCCTCTGGGGGCCTCCTCACCTTCATCATCCTGCTCAAG AATCAGATCAACCTCCTGGGCTTCACCCTGATGTTCTGGTGTGAATTCACTGCCTCCTTCCTCTTCTTCCTCAATGCCGC CAGCGGCCTTCACATCAACAGCCTCACTCAGCCCTGGGACCCTCCAGCAGGGACCCTGGCTTACAGAAAGCGAGGTTATG ATGGGACTTCTCTGATATAGGACCTCTGGCTGAGATTGAATAGGACAΆCCAATGACCTTGACACTGCCTCTTΆGGCACTT AGCTCTAGCAATGCCCTGGAAGTCTCTTCAGCTGAGCTCCAGGGCAAAGGCAGAAGGGTGCCTCTGTACGACGGCACAGT GAGCTGGATAGGTTAGTCATGCTATTAAAATCTCAATTTGAAAGTAAAAAAAAAAAAAAAAGGCGCTGCCTACCAGAGCGCAGCATGACGGCCΆTCGGCGCGCAGGCCCACAAGCTGTTGGGCCTTAAGAGGCCCCACCG GTCTTTCTTTGAGTCCTTCATCCGGACACTCATCATCGTGTGCACTGCCCTGGCTGTGGTCCTTTCTTCAGTCTCCATCT GCGATGGCCACTGGCTCCTAGTGGAGGATCATCTCTTTGGGCTGTGGTACTTCTGCACCATCGGCAACCACAGTGAACCA CACTGTCTGAGAGACCTGAGCCAGGCCCATATGCCCGGGCTGGCTGTAGGCATGGGCCTAGCACGCAGTGTGGCCGCCAT GGCAGTGGTGGCTGCCATCTTCGGCTTGGAGATGCTCATTGTGTCCCAGGTGTGTGAAGATGTCCGCTCACGGCGCAÄGT GGGCCATCGGTTCCTACCTCCTCCTGGTTGCCTTTATCCTCTCCTCTGGGGGCCTCCTCACCTTCATCATCCTGCTCAAG AATCAGATCAACCTCCTGGGCTTCACCCTGATGTTCTGGTGTGAATTCACTGCCTCCTTCCTCTTCTTCCTCAATGCCGC CAGCGGCCTTCACATCAACAGCCTCACTCAGCCCTGGGACCCTCCAGCAGGGACCCTGGCTTACAGAAAGCGAGGTTATG ATGGGACTTCTCTGATATAGGACCTCTGGCTGAGATTGAATAGGACAΆCCAATGACCTTGACACTGCCTCTTΆGGCACTT AGCTCTAGCAATGCCCTGGAAGTCTCTTCAGCTGAGCTCCAGGGCAAAGGCAGAAGGGTGCCTCTGTACGACGGCACAGT GAGCTGGATAGGTTAGTCATGCTATTAAAATCTCAATTTGAAAGTAAAAAAAAAAAAAAA
Klon 0005-ContigClone 0005 contig
AGCAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGC CATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACTT TGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTCACGGCAAGAΆGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACC TCGATGACCTGCCCGGTGCCCTGTCTGCTCTGAGCGACCTGCATGCCCACAAGCTGCGTGTGGATCCCGTCAACTTCAAG CTCCTGAGCCACTGCCTGCTGGTGACCTTGGCTAGCCACCACCCTGCCGATTTCACCCCCGCGGTGCATGCCTCTCTGGA CAAATTCCTTGCCTCTGTGAGCACCGTGCTGACCTCCAAGTACCGTAAGCTGCCTTNTGCGGGGCTTGCCTTCTGGCCAT GCCCTTCTTCTCTCCTTGCACCTGTACCTCTTTGGTTTTGAATAAAGCCTGAGTAGGAAGAAAAAAAAAAAAAAAAGCAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGC CATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACTT TGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTCACGGCAAGAΆGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACC TCGATGACCTGCCCGGTGCCCTGTCTGCTCTGAGCGACCTGCATGCCCACAAGCTGCGTGTGGATCCCGTCAACTTCAAG CTCCTGAGCCACTGCCTGCTGGTGACCTTGGCTAGCCACCACCCTGCCGATTTCACCCCCGCGGTGCATGCCTCTCTGGA CAAATTCCTTGCCTCTGTGAGCACCGTGCTGACCTCCAAGTACCGTAAGCTGCCTTNTGCGGGGCTTGCCTTCTGGCCAT GCCCTTCTTCTCTCCTTGCACCTGTACCTCTTTGGTTTTGAATAAAGCCTGAGTAGGAAGAAAAAAAAAAAAAAA
Klon 0006-T7-SequenzClone 0006-T7 sequence
AGGGGCTTCGGACCCGGAAGTGGCGCCTTGGGCTCCCGGCGGCGCCGCGGGGATGGCGGGAGCCGGAGCTGGTGCAGGAG CTCGGGGCGGCGCGCCGGCCGGAGTCGAGGCCCGCGCTCGGGACCCGCCACCCGCGCACCGCGCGCACCCTCGCCATCCT CGGCCCGCGGCTCAGCCGTCGGCGCGCAGGATGGACGGCGGCCCGGGCGCCCCGGGCTCCGGGGACAACGCCCCGACCAC CGAGGCGCTGTTCGTGGCGCTGGGCGCGGGCGTGACGGCTCTCAGTCACCCGCTGCTCTACGTGAAGCTGCTGATCCAGG TGGGTCATGAGCCGATGCCCCCCACCCTTGGGACCAATGTGCTGGGGAGGAAGGTCCTCTACCTGCCGAGCTTCTWCAAC CTATGCCAAGTACATTGTGCAGGTGGATGGGAAGATAGGGCTCTTCCGGGGCCTGAGCCCCCGCCTTATGTCCAACGCCT TGTCCACTGTGACCCGCGGCAGCATGAAGAAGGTTTTCCCTCCAGATGAGATGGAGCAGGTTTCCAAACAAGGACGACAT GAAGACCTCACTCAAGAAAGTTGTGAAGGAGACATCGTATGAGATGATGATGCAGTGTGTATCGCGAATGCTGGCCCATC CCTTACACGTGATCTCGATGCGATGCATGGTGCAGTTTGTGGGACGGGAGGCCAΆGTACAGTGGTGTGCTGAGTTCTATT GGGAAGATCTTCAAGGAAGAGGGGCTGCTGGGATTCTTCGTTGGCTTAATCCCTCACCTCCTGGGCGATGTGGTTTTCTT GTGGGGCTGTAACCTGCTGGCCCACTTCATCAATGCCTACTTGGTGGACGACAGCTTTAGCCAGGCCCTGGCCATCCGGA GCTACACCAAGTTTGTGATGGGGATTGCAGTGAGCATGCTGACCTACCCCTTCCTGCTCGTTGGAGATCTCATGGCAGTG AACAACTGTGGGCTGCGGGCTGGACTCCCTCCGAATTCCCCTGAGTTCAAGTCAGGGGCTTCGGACCCGGAAGTGGCGCCTTGGGCTCCCGGCGGCGCCGCGGGGATGGCGGGAGCCGGAGCTGGTGCAGGAG CTCGGGGCGGCGCGCCGGCCGGAGTCGAGGCCCGCGCTCGGGACCCGCCACCCGCGCACCGCGCGCACCCTCGCCATCCT CGGCCCGCGGCTCAGCCGTCGGCGCGCAGGATGGACGGCGGCCCGGGCGCCCCGGGCTCCGGGGACAACGCCCCGACCAC CGAGGCGCTGTTCGTGGCGCTGGGCGCGGGCGTGACGGCTCTCAGTCACCCGCTGCTCTACGTGAAGCTGCTGATCCAGG TGGGTCATGAGCCGATGCCCCCCACCCTTGGGACCAATGTGCTGGGGAGGAAGGTCCTCTACCTGCCGAGCTTCTWCAAC CTATGCCAAGTACATTGTGCAGGTGGATGGGAAGATAGGGCTCTTCCGGGGCCTGAGCCCCCGCCTTATGTCCAACGCCT TGTCCACTGTGACCCGCGGCAGCATGAAGAAGGTTTTCCCTCCAGATGAGATGGAGCAGGTTTCCAAACAAGGACGACAT GAAGACCTCACTCAAGAAAGTTGTGAAGGAGACATCGTATGAGATGATGATGCAGTGTGTATCGCGAATGCTGGCCCATC CCTTACACGTGATCTCGATGCGATGCATGGTGCAGTTTGTGGGACGGGAGGCCAΆGTACAGTGGTGTGCTGAGTTCTATT GGGAAGATCTTCAAGGAAGAGGGGCTGCTGGGATTCTTCGTTGGCTTAATCCCTCACCTCCTGGGCGATGTGGTTTTCTT GTGGGGCTGTAACCTGCTGGCCCACTTCATCAATGCCTACTTGGTGGACGACAGCTTTAGCCAGGCCCTGGCCATCCGGA GCTACACCAAGTTTGTGATGGGGATTGCAGTGAGCATGCTGACCTACCCCTTCCTGCTCGTTGGAGATCTCATGGCAGTG AACAACTGTGGGCTGCGGGCTGGACTC CCTCCGAATTCCCCTGAGTTCAAGTC
Klon 0006-BGH-SequenzClone 0006 BGH sequence
GAGCAGTGTTGGGGTGGCATCCCCCTTCCTGCCTAGAGGTACTGGAGTCCATCTTGTACTCAGGCAGAGGCAGGCTGCAG AGGCAAACGTCACTCAGTGGCAAGGCTTCCCTGCACCTCTAGCCCAGCTCATCCTGCCAGTCAGCCAGAAGCACCCCCGC CCCCCACTTCCTGCTTTGTAAATTGGGCGCCATCACACCTGGGCCATGGGAGGCTGGCGCTATGTTCCCAACACTAATTT TCTTATACAAGGGTGGTGCCTTCTCCTGAATAGGAAATCATGTTCTCCTCAGACCATCCCCTCATCTGCTTGTCTGTGCT GGTGACGCCAGGTGTGAGGGTTCAGTCACTGTGCTGGGTGCGAATACGCACAGGTTACATAGGCCGACÄTCTAGTCCTCC CCTCGTGGTAAGATAGACCCATCTCCTCGAATAΑATGTATTGGTGGTGATTTGGAAAAAAAAAAAAÄAAAAAGAGCAGTGTTGGGGTGGCATCCCCCTTCCTGCCTAGAGGTACTGGAGTCCATCTTGTACTCAGGCAGAGGCAGGCTGCAG AGGCAAACGTCACTCAGTGGCAAGGCTTCCCTGCACCTCTAGCCCAGCTCATCCTGCCAGTCAGCCAGAAGCACCCCCGC CCCCCACTTCCTGCTTTGTAAATTGGGCGCCATCACACCTGGGCCATGGGAGGCTGGCGCTATGTTCCCAACACTAATTT TCTTATACAAGGGTGGTGCCTTCTCCTGAATAGGAAATCATGTTCTCCTCAGACCATCCCCTCATCTGCTTGTCTGTGCT GGTGACGCCAGGTGTGAGGGTTCAGTCACTGTGCTGGGTGCGAATACGCACAGGTTACATAGGCCGACÄTCTAGTCCTCC CCTCGTGGTAAGATAGACCCATCTCCTCGAATAΑATGTATTGGTGGTGATTTGGAAAAAAAAAAAAÄAAAAA
Klon 0007-ContigClone 0007 contig
AGCTCCGCCCCTGCTACTGGACCATGGARACTGTGGCCCAGTAGAGACCTTAGTGTGAGGCTTTCAGGGGCGGCGGCCATAGCTCCGCCCCTGCTACTGGACCATGGARACTGTGGCCCAGTAGAGACCTTAGTGTGAGGCTTTCAGGGGCGGCGGCCAT
GGAGGCCGTGCTGAACGAGCTGGTGTCTGTGGAGGATCTGAAGAATTTTGAAAGGAAATTTCAGTCTGAGCAGGCAGCTGGGAGGCCGTGCTGAACGAGCTGGTGTCTGTGGAGGATCTGAAGAATTTTGAAAGGAAATTTCAGTCTGAGCAGGCAGCTG
GTTCTGTGTCCAAGAGCACGCAATTTGAATATGCCTGGTGCCTGGTTCGAAGCAAATACAATGAGGACATCCGCAGAGGCGTTCTGTGTCCAAGAGCACGCAATTTGAATATGCCTGGTGCCTGGTTCGAAGCAAATACAATGAGGACATCCGCAGAGGC
ATCGTGCTGCTGGAGGAGCTGTTGCCCAAAGGGAGCAAAGAGGAACAGCGGGACTATGTCTTCTACCTGGCCGTGGGCAAATCGTGCTGCTGGAGGAGCTGTTGCCCAAAGGGAGCAAAGAGGAACAGCGGGACTATGTCTTCTACCTGGCCGTGGGCAA
CTACCGGCTCAAGGAATATGAAAAGGCTCTAAAGTATGTGCGAGGGCTGTTGCAGACTGAGCCCCAGAACAACCAGGCCACTACCGGCTCAAGGAATATGAAAAGGCTCTAAAGTATGTGCGAGGGCTGTTGCAGACTGAGCCCCAGAACAACCAGGCCA
AGGAGCTGGAACGCCTGATTGATAAGGCCATGAAGAAAGATGGACTGGTAGGCATGGCCATCGTTGGTGGCATGGCCCTGAGGAGCTGGAACGCCTGATTGATAAGGCCATGAAGAAAGATGGACTGGTAGGCATGGCCATCGTTGGTGGCATGGCCCTG
GGCGTGGCAGGCCTGGCTGGACTCATTGGACTGGCTGTCTCCAAGTCCAAATCCTGAAGGCAGCCTCACCTGCTCTCTGCGGCGTGGCAGGCCTGGCTGGACTCATTGGACTGGCTGTCTCCAAGTCCAAATCCTGAAGGCAGCCTCACCTGCTCTCTGC
CCCGGGACGCCTAGGAGCCTGGGGGACACTGGAAGAGGGGCCTGTCCATCCTCACCATCGCCTTCCCTTTTCTCCTGCACCCCGGGACGCCTAGGAGCCTGGGGGACACTGGAAGAGGGGCCTGTCCATCCTCACCATCGCCTTCCCTTTTCTCCTGCAC
CCCTGTAGTCTACCTCTACAGTCTCCATGACCCCCAGCCTYTTAGCCCCTGCACCTGTCGTTTAACCCTGTCATGCTTTGCCCTGTAGTCTACCTCTACAGTCTCCATGACCCCCAGCCTYTTAGCCCCTGCACCTGTCGTTTAACCCTGTCATGCTTTG
CAATGAGTGTAAATAAAATTGGGCCGTGGCTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA Klon 0008-ContigCAATGAGTGTAAATAAAATTGGGCCGTGGCTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA Clone 0008 contig
AGATTGACTTGGGCACTGACATGGTTCCTGCCATCTCCCTGGCCTACGAGCAAGCTGAGAGCGACATCATGÄAGAGGCAG CCCAGAAACCCCAAAACGGACAAACTTGTGAACGAGCGTCTGATCAGCATGGCCTATGGACAGATCGGTATGATCCAGGC CCTGGGAGGCTTCTTCACTTACTTTGTGATTCTGGCTGAGAACGGTTTCCTGCCCTTTCACCTGTTGGGCATCCGAGAGA CCTGGGATGACCGCTGGGTCAACGATGTGGAGGACAGCTACGGGCAGCAGTGGACCTACGAGCAGAGGAAGATCGTGGAG TCACCTGCCATACAGCGTTCTTTGTCAGTATTGTGGTAGTGCAGTGGGCCGACTTGGTCATCTGCAAGACCAGAAGGAA TTCTGTCTTCCAGCAGGGAATGAAGAACAAGATCTTGATATTTGGCCTCTTTGAAGAGACAGCCCTTGCTGCTTTCTTAT CCTACTGCCCCGGGATGGGGGCAGCCCTTAGGATGTATCCCCTCAAACCTACATGGTGGKTCTGTGCCTTYCCCTACTCC CTTCTYATCTTTGTGTATGACGAGGTGCGGAAGCTYATTATYAGGCGGSGCCCTGGCGGCTGGGTGGAGAAGGAGACCTA CTACTAGCCCYACTGCCCTGSACGCCGKGGAACATTGTGCCACACACTGCACCCACCCCWACCCCCTCTWYSYSYMCTTY AAGTYTTGGAGCTCGGRACTCTWCCCTSRWAGGRWWKYWSSRARGCMTKTGGGRATKYWKΆCSYCYTGGRATGRAGSAWR TAGCTGYAMCSSRRRRCSGKKYGKKGGGGAGGRYTGCCTGAGAAACNCCGTGTMCKKGGACGACAKCGGRMAAGRATTK TACGTGCCTTTTTGTTTTTGTAAAAAAGGAAAACCTGGAAAGACTGAAAGATTACGTTTTATATCTGGATTTTTACAAAT AAAGATGGCTATTATAACGGAGATTGACTTGGGCACTGACATGGTTCCTGCCATCTCCCTGGCCTACGAGCAAGCTGAGAGCGACATCATGÄAGAGGCAG CCCAGAAACCCCAAAACGGACAAACTTGTGAACGAGCGTCTGATCAGCATGGCCTATGGACAGATCGGTATGATCCAGGC CCTGGGAGGCTTCTTCACTTACTTTGTGATTCTGGCTGAGAACGGTTTCCTGCCCTTTCACCTGTTGGGCATCCGAGAGA CCTGGGATGACCGCTGGGTCAACGATGTGGAGGACAGCTACGGGCAGCAGTGGACCTACGAGCAGAGGAAGATCGTGGAG TCACCTGCCATACAGCGTTCTTTGTCAGTATTGTGGTAGTGCAGTGGGCCGACTTGGTCATCTGCAAGACCAGAAGGAA TTCTGTCTTCCAGCAGGGAATGAAGAACAAGATCTTGATATTTGGCCTCTTTGAAGAGACAGCCCTTGCTGCTTTCTTAT CCTACTGCCCCGGGATGGGGGCAGCCCTTAGGATGTATCCCCTCAAACCTACATGGTGGKTCTGTGCCTTYCCCTACTCC CTTCTYATCTTTGTGTATGACGAGGTGCGGAAGCTYATTATYAGGCGGSGCCCTGGCGGCTGGGTGGAGAAGGAGACCTA CTACTAGCCCYACTGCCCTGSACGCCGKGGAACATTGTGCCACACACTGCACCCACCCCWACCCCCTCTWYSYSYMCTTY AAGTYTTGGAGCTCGGRACTCTWCCCTSRWAGGRWWKYWSSRARGCMTKTGGGRATKYWKΆCSYCYTGGRATGRAGSAWR TAGCTGYAMCSSRRRRCSGKKYGKKGGGGAGGRYTGCCTGAGAAACNCCGTGTMCKKGGACGACAKCGGRMAAGRATTK TACGTGCCTTTTTGTTTTTGTAAAAAAGGAAAACCTGGAAAGACTGAAAGATTACGTTTTATATCTGGATTTTTACAAAT AAAGATGGCTATTATAACGG
Klon 0009-T7-SequenzClone 0009-T7 sequence
AGCCGGAGGCCGAGCCCAGTCGCCAGCTCCTGCTCTGCTCCTCTCCCGCCTGCCGCCGCGCTGCACGCCTCGAGCACTCC CTCGGCCCCGGCGGGGACCGGGGACCCCGCAGCTACCGCCATGCTGCCAGTGCTCTACACCGGCCTGGCGGGGCTGCTGC TGCTGCCTCTGCTGCTCACCTGCTGCTGCCCCTACCTCCTCCAAGATGTGCGGTACTTCCTGCGGCTGGCCAACATGGCC CGGCGGGTGCGCAGCTACCGGCAGCGGCGACCCGTGCGTACCATCCTGCGGGCCTTCCTGGAACAAGCGCGCAAGACCCC ACACAAGCCCTTCCTGCTGTTCCGAGACGAGACGCTCACCTACGCCCAGGTGGACCGGCGCAGCAACCAAGTGGCGCGGG CGCTGCACGATCAACTGGGCCTACGACAGGGGGATTGCGTAGCCCTCTTCATGGGCAATGAGCCGGCCTACGTGTGGATC TGGCTGGGACTGCTCAAACTGGGCTGTCCCATGGCGTGCCTCAACTACAACATTCGTGCCAAGTCTCTGCTGCACTGCTT TCAATGCTGCGGGGCGAAGGTGCTGCTGGCCTCCCCAGATCTACAAGAAGCTGTGGAGGAGGTTCTTCCAACCCTGAAAA ANGATGCCGTGTNCCGTCTTTTACGTAAGCAGAACTTCTAACACAAATGGTGTGGACACAATACTGGACAAAAGTAGAAGCCGGAGGCCGAGCCCAGTCGCCAGCTCCTGCTCTGCTCCTCTCCCGCCTGCCGCCGCGCTGCACGCCTCGAGCACTCC CTCGGCCCCGGCGGGGACCGGGGACCCCGCAGCTACCGCCATGCTGCCAGTGCTCTACACCGGCCTGGCGGGGCTGCTGC TGCTGCCTCTGCTGCTCACCTGCTGCTGCCCCTACCTCCTCCAAGATGTGCGGTACTTCCTGCGGCTGGCCAACATGGCC CGGCGGGTGCGCAGCTACCGGCAGCGGCGACCCGTGCGTACCATCCTGCGGGCCTTCCTGGAACAAGCGCGCAAGACCCC ACACAAGCCCTTCCTGCTGTTCCGAGACGAGACGCTCACCTACGCCCAGGTGGACCGGCGCAGCAACCAAGTGGCGCGGG CGCTGCACGATCAACTGGGCCTACGACAGGGGGATTGCGTAGCCCTCTTCATGGGCAATGAGCCGGCCTACGTGTGGATC TGGCTGGGACTGCTCAAACTGGGCTGTCCCATGGCGTGCCTCAACTACAACATTCGTGCCAAGTCTCTGCTGCACTGCTT TCAATGCTGCGGGGCGAAGGTGCTGCTGGCCTCCCCAGATCTACAAGAAGCTGTGGAGGAGGTTCTTCCAACCCTGAAAA ANGATGCCGTGTNCCGTCTTTTACGTAAGCAGAACTTCTAACACAAATGGTGTGGACACAATACTGGACAAAAGTAGA
Klon 0009-BGH-SequenzClone 0009 BGH sequence
TTTTTTTTTTTTTTTAGTGTTAATATAGTTTATTATGTCTTTAAAAAAATAAGGCCCTCTCTCCAAGAAGCTTAGTTTGCTTTTTTTTTTTTTTTAGTGTTAATATAGTTTATTATGTCTTTAAAAAAATAAGGCCCTCTCTCCAAGAAGCTTAGTTTGC
AAGGACAAATGGCAGGTGCACATTGAAAAATAATTGTTTCTAAATCTTTTTACTTGCAAAGGTTCAGGTGTAATTTAAAAAAGGACAAATGGCAGGTGCACATTGAAAAATAATTGTTTCTAAATCTTTTTACTTGCAAAGGTTCAGGTGTAATTTAAAA
AAAAAACAAACAAACTATCCTTTTAATGAATAATTTCCTAAAAATAAAATCGCACCTTATAGCCTTCAATCAAGTTAAAGAAAAAACAAACAAACTATCCTTTTAATGAATAATTTCCTAAAAATAAAATCGCACCTTATAGCCTTCAATCAAGTTAAAG
TTGGATTCTACGTATGAAGTGGCTCTGCGAGGTCTATCGAGTTTCTTTCTGGAAATGTCATGAGCTAAACCACCAGGGAATTGGATTCTACGTATGAAGTGGCTCTGCGAGGTCTATCGAGTTTCTTTCTGGAAATGTCATGAGCTAAACCACCAGGGAA
TATTCAGAGCTTCAGAGTTTTATCAATTATGGCATTATAAATGTTCTCAGTCATGGGCACAAATGTTTTCTCTGCATCATTATTCAGAGCTTCAGAGTTTTATCAATTATGGCATTATAAATGTTCTCAGTCATGGGCACAAATGTTTTCTCTGCATCAT
CCATGAAATACAAGGTATCTTTGATGACTGTGGGATTGAAGCCCTCTTCCATCAGGGTCACTTTGCGGTGTTTAAAAGTCCCATGAAATACAAGGTATCTTTGATGACTGTGGGATTGAAGCCCTCTTCCATCAGGGTCACTTTGCGGTGTTTAAAAGTC
CCAGTGATCTCAATGGTATCTTGTATCCTCAGGAACCGAGGCCTCGCGTAACTGGGCAGGTACTCCGCGATGTGTTGAAACCAGTGATCTCAATGGTATCTTGTATCCTCAGGAACCGAGGCCTCGCGTAACTGGGCAGGTACTCCGCGATGTGTTGAAA
GAGTTTCTTTCCATTGAACTCGTAGTTTTCTTTGATCTTGAGAGTTTCTTTCCATTGAACTCGTAGTTTTCTTTGATCTTGA
Klon 0010-ContigClone 0010 contig
AGCGCGGGAGGGGCATGGCGGGCATGGCGCTGGCGCGAGCATGGAAGCAGATGTCCTGGTTCTACTACCAGTACCTGCTGAGCGCGGGAGGGGCATGGCGGGCATGGCGCTGGCGCGAGCATGGAAGCAGATGTCCTGGTTCTACTACCAGTACCTGCTG
GTCACTGCGCTCTACATGCTGGAGCCCTGGGAGCGAACCGTGTTCAATTCGATGCTGGTTTCCGTGGTGGGGATGGCCCTGTCACTGCGCTCTACATGCTGGAGCCCTGGGAGCGAACCGTGTTCAATTCGATGCTGGTTTCCGTGGTGGGGATGGCCCT
GTACACTGGCTACGTCTTCATGCCCCAGCACATCATGGCTATTCTGCATTACTTTGAAATTGTACAGTGACGAAGATGTGGTACACTGGCTACGTCTTCATGCCCCAGCACATCATGGCTATTCTGCATTACTTTGAAATTGTACAGTGACGAAGATGTG
ACCAGGATCCAGAGGTTCCTGGGGAAGATCTGCCTTGTGAAGTTGGAATGAGACCTCATCAGATGTAAGATGTGCTACGGACCAGGATCCAGAGGTTCCTGGGGAAGATCTGCCTTGTGAAGTTGGAATGAGACCTCATCAGATGTAAGATGTGCTACGG
ATGTCCACGTGACCAACCTTATAAATACAAAGACTTTAAAAAAAACTTCATGAGTAGAACAGGAAAAATCATCCTGGCTCATGTCCACGTGACCAACCTTATAAATACAAAGACTTTAAAAAAAACTTCATGAGTAGAACAGGAAAAATCATCCTGGCTC
ATGTGTTG GTTCTTTCTTTTTGATTTTAACAGAGGCTCTTATATAGTAGCTTTTATCTATTTTAACATTGTAGTCATTTATGTGTTG GTTCTTTCTTTTTGATTTTAACAGAGGCTCTTATATAGTAGCTTTTATCTATTTTAACATTGTAGTCATTT
GTACTTTGATATCAGTATTTTCTTAACCTTTGTGACTGTTTCAATATTATCCAGTGAAAGCTTTTCTTAATGTAACTTTGGTACTTTGATATCAGTATTTTCTTAACCTTTGTGACTGTTTCAATATTATCCAGTGAAAGCTTTTCTTAATGTAACTTTG
AGTACATCTCAATTGCCTTCTATTTTTAAAACCTAAGGTCATTAGTTGGGCTTTACTGTTCTTGCTATCATATGGCATATAGTACATCTCAATTGCCTTCTATTTTTAAAACCTAAGGTCATTAGTTGGGCTTTACTGTTCTTGCTATCATATGGCATAT
ACATCTGCCTGGATATATTTCTACTCTTGACCAAAGTTTTGTAAGAAACAATACAGAATTTGGGGACGGGAGGGAGGATAACATCTGCCTGGATATATTTCTACTCTTGACCAAAGTTTTGTAAGAAACAATACAGAATTTGGGGACGGGAGGGAGGATA
CTTTGAGAACTACTTACAAAAGATTTATCTGCTTGCTTGAACTCAGGAGTACGGTTTTAGCTCCCTAGACTCTTAACAGCCTTTGAGAACTACTTACAAAAGATTTATCTGCTTGCTTGAACTCAGGAGTACGGTTTTAGCTCCCTAGACTCTTAACAGC
TTTTGCTCTAAAACTATTAAAGTGTTTCTTAGTAATGAAAAAGTAAAGATCTTGCTAACATTAATACAAGGAACATTTCNTTTTGCTCTAAAACTATTAAAGTGTTTCTTAGTAATGAAAAAGTAAAGATCTTGCTAACATTAATACAAGGAACATTTCN
CCTTTTAGATATTAAATTCTTATGTGGACTTATTTCCAACCAACTTTGATGATTCCTGTGATTGGTGATTGGTGGCCGGCCCTTTTAGATATTAAATTCTTATGTGGACTTATTTCCAACCAACTTTGATGATTCCTGTGATTGGTGATTGGTGGCCGGC
TTGTATGACATATGCTGAGATACACTGTGGAGCTTTTCATAGAAGGCTTATCAGATCCTCTGAGGCAGTATTGACCCTGTTTGTATGACATATGCTGAGATACACTGTGGAGCTTTTCATAGAAGGCTTATCAGATCCTCTGAGGCAGTATTGACCCTGT
ATACGCTCAAGAGTTAATTTGATGTTGTAACAGGCGTTGTGAAATGCATTTGCACGTTGTGATAAAGACMCAATGAGATGATACGCTCAAGAGTTAATTTGATGTTGTAACAGGCGTTGTGAAATGCATTTGCACGTTGTGATAAAGACMCAATGAGATG
AATTGTGCAGAACCATAACCAAAAGAAATGTAAACTTGCTTAAAAATCCTTTCACTCTTTGTATTTTTTTTAAAACGGTTAATTGTGCAGAACCATAACCAAAAGAAATGTAAACTTGCTTAAAAATCCTTTCACTCTTTGTATTTTTTTTAAAACGGTT
TTTATTCCTTAGATGTAAAATGACTACTTTTTTTTGATGTAAACTCATTAAATTCAAAGAAAAATGTAAAAAAAAAAAAATTTATTCCTTAGATGTAAAATGACTACTTTTTTTTGATGTAAACTCATTAAATTCAAAGAAAAATGTAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁ
Klon OOU-ContigClone OOU-Contig
AGCGAGTTTGCAGACTTCTTGTGCGCAGCTAGCCGCCTNAGGTGTTNGAACCATGAÄTCTTTTACTCCTTTNGGCTGTCC TCTGCTTGGGAACAGCCTTAGCTACTCCAAAATTTGATNAAACCTTTAGTGCANAGTGGCACCAGTGGAAGTCNACGCAC AGAAGACTGTATGGCACGAATGAGGAANAGTGGAGGANAGCGATATGGGAGAAGAACATGAGAATGATCCAGCTACACAA CGGGGAATACAGCAACGGGCAGNACGGCTTTTCCATGGAGATGAACGCCTTTGGTGACATGACCAATGAGGAATTCAGGC AGGNGGNGAATGGCTATCGCCACCAGAAGCACAAGAAGGGGAGGCTTTTTCAGGAACCGCTGATGCTTAAGATCCCCAAG TCTGNGGACTGGAGAGAAAAGGGTTGNGTGACTCCTGTGAAGAACCAGGGCCAGNGCGGGTCTNGNNGGGCGTTTAGCGC ATCGGGTTGCCTAGAAGGACAGATGTTCCTTAAGACCGGCAAACTGATCTNACTGAGTNAACAGAACCTTGTGGACTGTT CTCACGCTCAAGGCAATCAGGGCTGTAACGGAGGCCTGATGGATTTTGCTTTCCAGTACATTAAGGAAAATGGAGGTCTG NACTCGGAGGAGTCTTACCCCTATGAAGCAAAGGACNGG TCTTGTAAATACAGAGCCGAGTTCSCTGTGGCTAATGACA CAGGGTTCCGTGGATATCCCTYAGCMAGGAGAAAGCCCTCATGAAGGCTGTGCGACTGTGGGGCCTATTTCTGTTGCTAT GGACGCAAGCCATCCGTCTCTCCAGTTCTATAGTTCAGGCATCTACTATNAA.CCCNACTGTAGCAGCAAGATCCTCGACC ATGGGGTTCTGTTGNTNGGCTATGGCTATNAAGGANCAGATTCAAATAAGAATAAATATTGGCTTGTCAAGANCAGCTGG GGAAGTGAATGGGGTATGGAAGGCTACATCNAAATAGCCNAAGACCGGGNCNACCTCTGTGGACTTGCCNCCGCGNCCAN CTATCCTGTCNTNAATTNATNGGTAGCGNTAATGAGGACTTATGGNCTCTATGTCCNAAGGAATTCAGCTTAAAACTGAC CNAACCCTTATTNANTCNAACCNTGGTNCTTNAATCATTNAGGATCCAANTCATNATTTNAATTCTGTTGCCATTTTTAC ATGGGTTAAATGTTACCNCTACTTAAAACTCCTNTTATAAACANCTTTATAATATTGAAAACTTAGTGCTTAATTCTNAG TCTNNAATATTTNTTTTATATAAAGGTTGTATAAAACTTTCTTTACCTCTTAAAAATAAATTTTAGCTCAGTGTGTGTGT AAAGCGAGTTTGCAGACTTCTTGTGCGCAGCTAGCCGCCTNAGGTGTTNGAACCATGAÄTCTTTTACTCCTTTNGGCTGTCC TCTGCTTGGGAACAGCCTTAGCTACTCCAAAATTTGATNAAACCTTTAGTGCANAGTGGCACCAGTGGAAGTCNACGCAC AGAAGACTGTATGGCACGAATGAGGAANAGTGGAGGANAGCGATATGGGAGAAGAACATGAGAATGATCCAGCTACACAA CGGGGAATACAGCAACGGGCAGNACGGCTTTTCCATGGAGATGAACGCCTTTGGTGACATGACCAATGAGGAATTCAGGC AGGNGGNGAATGGCTATCGCCACCAGAAGCACAAGAAGGGGAGGCTTTTTCAGGAACCGCTGATGCTTAAGATCCCCAAG TCTGNGGACTGGAGAGAAAAGGGTTGNGTGACTCCTGTGAAGAACCAGGGCCAGNGCGGGTCTNGNNGGGCGTTTAGCGC ATCGGGTTGCCTAGAAGGACAGATGTTCCTTAAGACCGGCAAACTGATCTNACTGAGTNAACAGAACCTTGTGGACTGTT CTCACGCTCAAGGCAATCAGGGCTGTAACGGAGGCCTGATGGATTTTGCTTTCCAGTACATTAAGGAAAATGGAGGTCTG NACTCGGAGGAGTCTTACCCCTATGAAGCAAAGGACNGG TCTTGTAAATACAGAGCCGAGTTCSCTGTGGCTAATGACA CAGGGTTCCGTGGATATCCCTYAGCMAGGAGAAAGCCCTCATGAAGGCTGTGCGACTGTGGGGCCTATTTCTGTTGCTAT GGACGCAAGCCATCCGTCTCTCCAGTTCTATAGTTCAGGCATCTACTATNAA.CCCNACTGTAGCAGCAAGATCCTCGACC ATGGGGTTCTGTTGNTNGGCTATGGCTATNAAGGANCAGATTCAAATAAGAATAAATATTGGCTTGTCAAGANCAGCTGG GGAAGTGAATGGGGTATGGAAGGCTACATCNAAATAGCCNAAGACCGGGNCNACCTCTGTGGACTTGCCNCCGCGNCCAN CTATCCTGTCNTNAATTNATNGGTAGCGNTAATGAGGACTTATGGNCTCTATGTCCNAAGGAATTCAGCTTAAAACTGAC CNAACCCTTATTNANTCNAACCNTGGTNCTTNAATCATTNAGGATCCAANTCATNATTTNAATTCTGTTGCCATTTTTAC ATGGGTTAAATGTTACCNCTACTTAAA ACTCCTNTTATAAACANCTTTATAATATTGAAAACTTAGTGCTTAATTCTNAG TCTNNAATATTTNTTTTATATAAAGGTTGTATAAAACTTTCTTTACCTCTTAAAAATAAATTTTAGCTCAGTGTGTGTGT AA
Klon 0012-ContigClone 0012 contig
GAGCGCCGCCGGGTCGGCCAGCGAGGCCTCAGAGGTTCCAGACAACGTGGGAGACTGGCTCCGCGGCG CTTCCGCTTCGGAGCGCCGCCGGGTCGGCCAGCGAGGCCTCAGAGGTTCCAGACAACGTGGGAGACTGGCTCCGCGGCG CTTCCGCTTCG
CCACCGATCGAAACGACTTCCGGAGGAACTTGATCCTYAATTTGGGACTCTTTGCTGCGGGAGTCTGGCTGGCCAGGAACCCACCGATCGAAACGACTTCCGGAGGAACTTGATCCTYAATTTGGGACTCTTTGCTGCGGGAGTCTGGCTGGCCAGGAAC
TTGAGTGACATTGATTTGATGGCCCCTCAGCCAGGGGTGTAGCCAGAGAATGGAACTCCTGTGTATTCAGACTTTCCAAATTGAGTGACATTGATTTGATGGCCCCTCAGCCAGGGGTGTAGCCAGAGAATGGAACTCCTGTGTATTCAGACTTTCCAAA
GACAGCCTACTGTCTGTGACCACAAGATCCTACCTGAGTGGCAGCTGAAGTTGACTCCCTCTCCKTGCCTGAACCCCCCCGACAGCCTACTGTCTGTGACCACAAGATCCTACCTGAGTGGCAGCTGAAGTTGACTCCCTCTCCKTGCCTGAACCCCCCC
CC CTGCCCCCCCATCCCCCAGTGTCGGCTRAGATGTTGCCTCTGCACGGTTCTGTGTGCAGTTCCCNACTTTCTGCAGACC CTGCCCCCCCATCCCCCAGTGTCGGCTRAGATGTTGCCTCTGCACGGTTCTGTGTGCAGTTCCCNACTTTCTGCAGA
AGATGGTCCTTGCCCTTGTCCTNAAGAGTAATAATGGTTCTTGAAAAAGATTTCAAATAAAGCCTGCNCATAGATGGTCCTTGCCCTTGTCCTNAAGAGTAATAATGGTTCTTGAAAAAGATTTCAAATAAAGCCTGCNCAT
Klon 0014-T7-SequenzClone 0014-T7 sequence
AAAGGAAAAÄCACAGCTCAGCAGATCCAGGCACTAAAGAGAGCTAGCTGCAAGCAGGAGCAGTCAAGAATCTGTGGTCAGAAAGGAAAAÄCACAGCTCAGCAGATCCAGGCACTAAAGAGAGCTAGCTGCAAGCAGGAGCAGTCAAGAATCTGTGGTCAG
AAGTACTGGAGTGGGCCAGCAGGGCCAGCTTTTTCTACCATGGCAGCCCAAGGCTACGGCTACTATCGCACTGTCATATTAAGTACTGGAGTGGGCCAGCAGGGCCAGCTTTTTCTACCATGGCAGCCCAAGGCTACGGCTACTATCGCACTGTCATATT
TGCGGCCATGTTTGGAGGCTACAGCCTGTACTATTTCAACCGCAAAACCTTCTCCTTTGTCATGCCCTCCTTGGTGGATGTGCGGCCATGTTTGGAGGCTACAGCCTGTACTATTTCAACCGCAAAACCTTCTCCTTTGTCATGCCCTCCTTGGTGGATG
AGATCGCTCTGGACAAGGACGATTTGGGGCTCATCACAAGCAGCCAGTCGGCAGCCTACGCCATCAGCAAGTTTGTGAGCAGATCGCTCTGGACAAGGACGATTTGGGGCTCATCACAAGCAGCCAGTCGGCAGCCTACGCCATCAGCAAGTTTGTGAGC
GGGGTTCTGTCAGATCAGATGAGCGCCCGCTGGCTCTTCTCCTCTGGGCTGCTCCTGGTTGGTCTGGTCAACGTAGTCTTGGGGTTCTGTCAGATCAGATGAGCGCCCGCTGGCTCTTCTCCTCTGGGCTGCTCCTGGTTGGTCTGGTCAACGTAGTCTT
CTYATGGAGCTCCACAGTGTCAGCCTTWGCTGCTCTTTGGTTTCTTAATGGTCTGGCACAGGGGCTGGGCTGGCCCCCCTCTYATGGAGCTCCACAGTGTCAGCCTTWGCTGCTCTTTGGTTTCTTAATGGTCTGGCACAGGGGCTGGGCTGGCCCCCCT
GTGGGAAGATCCTGAGGAAGTGGTTTGAGCCATCCCAGTTTGGCACTTGGTGGGCTGTGTTGTCAACCAGCATGAACCTGGTGGGAAGATCCTGAGGAAGTGGTTTGAGCCATCCCAGTTTGGCACTTGGTGGGCTGTGTTGTCAACCAGCATGAACCTG
GCTGGAAGTTTGGGACCTATCTTGGCAACGATCCTCGCCCAGAGCTACAGCTGGCGCAGCACACTGGCCCTGTCTGGCTGGAAGTTTGGGACCTATCTTGGCAACGATCCTCGCCCAGAGCTACAGCTGGCGCAGCACACTGGCCCTGTCTG
Klon 0014-BGH-SequenzClone 0014 BGH sequence
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGDAACAAATCTAGCTTTATTGATCTATACAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGDAACAAATCTAGCTTTATTGATCTATACAA
GKCGGTTTGCCCTCTATATTCTTCCCTTTTGKCCCAGGGTATTCATGAGAGGGGGGGÄCYCCTYTYCTCCCTTAGGGACTGKCGGTTTGCCCTCTATATTCTTCCCTTTTGKCCCAGGGTATTCATGAGAGGGGGGGÄCYCCTYTYCTCCCTTAGGGACT
GRGGTATTGGCAACAATTTTGCCCCCTGCCAAGAAGCCTTGGGGTACAGCAGAAACCACAGGCCATTAATACTCACTAGGGRGGTATTGGCAACAATTTTGCCCCCTGCCAAGAAGCCTTGGGGTACAGCAGAAACCACAGGCCATTAATACTCACTAGG
AGATCAGGACCTAGGAGCAAGAAGAAGGGTATAGGAGACACTCTGAATTCAGGAGTTGCCTGTCTGCCAGAAGGKAGAAAAGATCAGGACCTAGGAGCAAGAAGAAGGGTATAGGAGACACTCTGAATTCAGGAGTTGCCTGTCTGCCAGAAGGKAGAAA
CAGAAGCTGA GGAGTCTAGAACCAAACATCATCATTTTAAATAGAGCATGGGAAGGGAGTCAGAAGCTGA GGAGTCTAGAACCAAACATCATCATTTTAAATAGAGCATGGGAAGGGAGT
Klon 0015-T7-SequenzClone 0015-T7 sequence
AGCGGAACTCAAGATGGCTGCGCTCTTGCTGAGTGCTGCTCCTTTGGGAACCACAGCTAAGGAGGAGATGGAGCGGTTCTAGCGGAACTCAAGATGGCTGCGCTCTTGCTGAGTGCTGCTCCTTTGGGAACCACAGCTAAGGAGGAGATGGAGCGGTTCT
GGAAGAAGAACACGAGTTCAAACCGTCCTCTGTCTCCCCATTTGACTATCTACAAATGGTCTCTTCCTATGGCACTGTCCGGAAGAAGAACACGAGTTCAAACCGTCCTCTGTCTCCCCATTTGACTATCTACAAATGGTCTCTTCCTATGGCACTGTCC
GTTTGCCACCGAGGCTCTGGAATAGCCTTGAGTGGAGGGGTCTCTCTTTTTGGCCTGTCGGCACTGGTGCTTCCTGGGAAGTTTGCCACCGAGGCTCTGGAATAGCCTTGAGTGGAGGGGTCTCTCTTTTTGGCCTGTCGGCACTGGTGCTTCCTGGGAA
CTTTGAGTCGTATTTGATGTTTGTGAAGTCCCTGTGTTTGGGGCCAACACTGATCTACTCGGCTAAGTTTGTGCTTGTCTCTTTGAGTCGTATTTGATGTTTGTGAAGTCCCTGTGTTTGGGGCCAACACTGATCTACTCGGCTAAGTTTGTGCTTGTCT
TCCCGCTCATGTACCACTCACTGAATGGGATCCGACACTTGCTATGGGACCTAGGAAAAGGCCTGGCAATACCCCAGGTCTCCCGCTCATGTACCACTCACTGAATGGGATCCGACACTTGCTATGGGACCTAGGAAAAGGCCTGGCAATACCCCAGGTC
TGGCTGTCTGGAGTGGCGGTCGTGGTTCTTGCTGTGTTGTCCTTGGCTGTCTGGAGTGGCGGTCGTGGTTCTTGCTGTGTTGTCCT
Klon 0015-BGH-SequenzClone 0015 BGH sequence
CTTYTTTTTTTTTTTTTTTTTTTTAAAAKGAGTAGTACTTTAATCGTTTCCAAAGAAGATGGTACATGAATTTAGATTAGCTTYTTTTTTTTTTTTTTTTTTTTAAAAKGAGTAGTACTTTAATCGTTTCCAAAGAAGATGGTACATGAATTTAGATTAG
AAAATATGACATTAAAGGTGTATTTTCCTAGAAATGAAAAACAGAACAGAAACTTAATACAATTTTTTTTGATCTTCCTCAAAATATGACATTAAAGGTGTATTTTCCTAGAAATGAAAAACAGAACAGAAACTTAATACAATTTTTTTTGATCTTCCTC
ATACATCAAAAGCTGTGTATCTGTTCCCAGGCTTGAGCTCCAAGCTCCCAGGCTGCTCTTTATCTCCCTCTAGCTGAGCAATACATCAAAAGCTGTGTATCTGTTCCCAGGCTTGAGCTCCAAGCTCCCAGGCTGCTCTTTATCTCCCTCTAGCTGAGCA
GGACAATCCCTCCTCCTCCACCTCTCTCCTCTCAGTCATGAGGTGTGGGTGCTAAAGGCCCTAAGCTGACCAATGGACAGGGACAATCCCTCCTCCTCCACCTCTCTCCTCTCAGTCATGAGGTGTGGGTGCTAAAGGCCCTAAGCTGACCAATGGACAG
AAGTGTCCTTTAAGCCAGCAGCCCACAGGCAGTGGCACTCGGGGCGGTCACAGAACTGGCATTGTTTMYACTGTCTCAGCAAGTGTCCTTTAAGCCAGCAGCCCACAGGCAGTGGCACTCGGGGCGGTCACAGAACTGGCATTGTTTMYACTGTCTCAGC
AGAAAGCAGCGGAAAGCTGACCCTCAGCACAAATCAGTCAAATGTGGAACCGGAGCAAGGGCTCCCAGGCCCAGGGAGGAAGAAAGCAGCGGAAAGCTGACCCTCAGCACAAATCAGTCAAATGTGGAACCGGAGCAAGGGCTCCCAGGCCCAGGGAGGA
TGACCCTTTCGTACTACAACAGGTCTTTAAGCCGCTCTACTGCACTCCAAGGGGATTGAAAGCATGTGGCATCTAAACAATGACCCTTTCGTACTACAACAGGTCTTTAAGCCGCTCTACTGCACTCCAAGGGGATTGAAAGCATGTGGCATCTAAACAA
ATCAGGAGAACCTGGGTGGT Klon 0016-ContigATCAGGAGAACCTGGGTGGT Clone 0016 contig
GAGGGGAACAAGATGGCGGCGCCAAAGGGGAAGCTTTGGGTCCAGGCCCAACTGGGGCTCCCGCCGCTGCTGCTGTTGACGAGGGGAACAAGATGGCGGCGCCAAAGGGGAAGCTTTGGGTCCAGGCCCAACTGGGGCTCCCGCCGCTGCTGCTGTTGAC
TATGGCGCTGGCCGGAGGCTCGGGGACTGCAGCGGCCGAAGCCTTTGACTCGGTCCTGGGAGACACAGCGTCCTGTCACCTATGGCGCTGGCCGGAGGCTCGGGGACTGCAGCGGCCGAAGCCTTTGACTCGGTCCTGGGAGACACAGCGTCCTGTCACC
GGGCCTGTCAGCTGACCTACCCCTTGCACACCTACCCGAAGGAAGAGGAGTTATACGCATGCCAGAGAGGCTGCAGGCTGGGGCCTGTCAGCTGACCTACCCCTTGCACACCTACCCGAAGGAAGAGGAGTTATACGCATGCCAGAGAGGCTGCAGGCTG
TTTTCAATTTGCCAGTTTGTGGATGATGGGCTTGATTTAAATCGGACCAAGCTGGAATGTGAATCTGCGTGCACAGAAGCTTTTCAATTTGCCAGTTTGTGGATGATGGGCTTGATTTAAATCGGACCAAGCTGGAATGTGAATCTGCGTGCACAGAAGC
ATATTCCCAACCTGATGAGCAGTATGCTTGTCATCTTGGCTGCCAGGATCAGTTGCCATTTGCTGAACTGAGACAAGAACATATTCCCAACCTGATGAGCAGTATGCTTGTCATCTTGGCTGCCAGGATCAGTTGCCATTTGCTGAACTGAGACAAGAAC
AACTCATGTCCCTGATGCCAAGAATGCATCTCCTCTTCCCTCTGACTCTGGTGAGGTCGTTCTGGAGTGACATGATGGACAACTCATGTCCCTGATGCCAAGAATGCATCTCCTCTTCCCTCTGACTCTGGTGAGGTCGTTCTGGAGTGACATGATGGAC
TCTGCACAGAGCTTCATAACCTCTTCATGGACTTTTTATCTTCAAGCCGATGACGGAAAAATAGTTATATTCCAGTCTAATCTGCACAGAGCTTCATAACCTCTTCATGGACTTTTTATCTTCAAGCCGATGACGGAAAAATAGTTATATTCCAGTCTAA
GCCAGAAATTCAGTATGCACCGCAGTTGGAGCAGGAGCCTACAAACTTGAGAGAATCATCTTTAAGCAAAATGTCCTATCGCCAGAAATTCAGTATGCACCGCAGTTGGAGCAGGAGCCTACAAACTTGAGAGAATCATCTTTAAGCAAAATGTCCTATC
TGCAGATGAGAAACTCACAAGCACACAGGAACTACCTTGAAGAGGAAGAAAGCGATGGCTTTTTAAGATGTCTATCTCTTTGCAGATGAGAAACTCACAAGCACACAGGAACTACCTTGAAGAGGAAGAAAGCGATGGCTTTTTAAGATGTCTATCTCTT
AACTCTGGATGGATTTTAACCACAACCCTTGTCCTCTCGGTGATGGTGTTGCTCTGGATCTGTTGTGCAGCTGTTGCTACAACTCTGGATGGATTTTAACCACAACCCTTGTCCTCTCGGTGATGGTGTTGCTCTGGATCTGTTGTGCAGCTGTTGCTAC
AGCTGTAGAACAGTATGTTCCCCCTGAGAAGCTGAGTATCTATGGTGACTTGGAATTTATGAATGAACAAAAGCTGAGCAAGCTGTAGAACAGTATGTTCCCCCTGAGAAGCTGAGTATCTATGGTGACTTGGAATTTATGAATGAACAAAAGCTGAGCA
GATACCCAGCTCCTTCTCTTGTGATTGTTAGGTCTCAGACTGAAGAACATGAGGAGGCAGGGCCCCTGCCCACCAAGGTGGATACCCAGCTCCTTCTCTTGTGATTGTTAGGTCTCAGACTGAAGAACATGAGGAGGCAGGGCCCCTGCCCACCAAGGTG
AACCTTGCTCACTCAGAAATCTAAGCTTTTTAAAAGAGTCGTGGACACATAAACTTCCATTCCTCATAGAGCTTTTTAAGAACCTTGCTCACTCAGAAATCTAAGCTTTTTAAAAGAGTCGTGGACACATAAACTTCCATTCCTCATAGAGCTTTTTAAG
ATGGTTTCATTGGACATAGGCCTTAAGAAATCACTATAAAATGCAAATAAAGTTACCAAACTCTGTGAAGACTTTATTTGATGGTTTCATTGGACATAGGCCTTAAGAAATCACTATAAAATGCAAATAAAGTTACCAAACTCTGTGAAGACTTTATTTG
CTGTGACTTTACCTGTATTTTTCTAGTCATTTAAGATGGACATTGGGTTGTATTTTTATTTTACTAATATCTGTAGCTACCTGTGACTTTACCTGTATTTTTCTAGTCATTTAAGATGGACATTGGGTTGTATTTTTATTTTACTAATATCTGTAGCTAC
TTAGTTAGTTGCATTGGTTTTGGTTTTTTTCCTCTCTTCGCCAAATTCTATGAGCTGATCATTGTGGCCCCGCCCCTGCCTTAGTTAGTTGCATTGGTTTTGGTTTTTTTCCTCTCTTCGCCAAATTCTATGAGCTGATCATTGTGGCCCCGCCCCTGCC
ATGCCCCCCGTCAGTCATCTCACTTAATAACCGAAACCTTAGGGTGTGATGCTTCTGCCCGGAAATGGCCTCCAAACTGTATGCCCCCCGTCAGTCATCTCACTTAATAACCGAAACCTTAGGGTGTGATGCTTCTGCCCGGAAATGGCCTCCAAACTGT
CCTCTGGATTATAGCAGAAATGTTATTTAATGACWCTACATTTTCAGTTGTATTGAATTGAAATCATTAAAATCTATTTGCCTCTGGATTATAGCAGAAATGTTATTTAATGACWCTACATTTTCAGTTGTATTGAATTGAAATCATTAAAATCTATTTG
AATAATTATGTTCTGAAAAAAAAAAAAAAAAAAAAAATAATTATGTTCTGAAAAAAAAAAAAAAAAAAAA
Klon 0017-T7-SequenzClone 0017-T7 sequence
AAAGGTACGAAGCTAGGGAAGATATTCGCGTGGCTAAATCTGCACGTGGAAGGAGCATTAACTTGGCCCTTTCTTATAGAAAAGGTACGAAGCTAGGGAAGATATTCGCGTGGCTAAATCTGCACGTGGAAGGAGCATTAACTTGGCCCTTTCTTATAGA
GGACGGCAGGCCTTGAAAGCCATTGGTCTGGAAGATCAGATCGTTTCCAAAGGTGTGCCCATGAAAGCCAGAATGATCCAGGACGGCAGGCCTTGAAAGCCATTGGTCTGGAAGATCAGATCGTTTCCAAAGGTGTGCCCATGAAAGCCAGAATGATCCA
CTCTCTTTCTGGAAAGAAGTCTGCAATTCCCTATGGGAACAAGTCACAGTATATCCTTTCAATAAGCAGAGAAAACTTAACTCTCTTTCTGGAAAGAAGTCTGCAATTCCCTATGGGAACAAGTCACAGTATATCCTTTCAATAAGCAGAGAAAACTTAA
ACAAGGACCTGCTGACTGCCGWGGAGTCCTATGCCAATGCGAAGGTGCACTTTGGCCACAAGCTGTCGAAATGCATTCCGACAAGGACCTGCTGACTGCCGWGGAGTCCTATGCCAATGCGAAGGTGCACTTTGGCCACAAGCTGTCGAAATGCATTCCG
GAGGAAGGGGTACTCACAGWGCTCGGACCTGACAAGGTTCCCCGAGATGTCACATGTGACCTTGTTGTAGGGTGTGATGGGAGGAAGGGGTACTCACAGWGCTCGGACCTGACAAGGTTCCCCGAGATGTCACATGTGACCTTGTTGTAGGGTGTGATGG
AGCCTATTCAACTGTCAGAGCCCACCTCATGAAGAAGCCCCGCTTTGATTACACTCAGCAATATATCCCTCATGGATACAAGCCTATTCAACTGTCAGAGCCCACCTCATGAAGAAGCCCCGCTTTGATTACACTCAGCAATATATCCCTCATGGATACA
TGGAGTTGACAATTCCACCTAAGAATGGGGAGTACGCCATGGAACCTAACTGTCTTCACATTTGGCCTAGAAATGCCTATTGGAGTTGACAATTCCACCTAAGAATGGGGAGTACGCCATGGAACCTAACTGTCTTCACATTTGGCCTAGAAATGCCTAT
ATGATGATCGCCCTTCCGAÄCATGGACAAATCTTTCACATGCACCTTGNTCATGCCCTTTGAGGAGTTTGAAAGACTTCCATGATGATCGCCCTTCCGAÄCATGGACAAATCTTTCACATGCACCTTGNTCATGCCCTTTGAGGAGTTTGAAAGACTTCC
AACGCGCAGCGATGTGCTGGACTTCTTCCAGAAGAACTTTNCAGATGCTATCCCTCTGATGGGAGAGCAAGCCCTCATGAAACGCGCAGCGATGTGCTGGACTTCTTCCAGAAGAACTTTNCAGATGCTATCCCTCTGATGGGAGAGCAAGCCCTCATGA
GAGAGAGA
Klon 0018-ContigClone 0018 contig
CCCTTTTNNCCCCAATTGGGCCGTTAGGCGTTTCCGGTGGAAAGGTCCTANNTTAAGCAGANGCCNCTTCGGGGCTAACCCCCTTTTNNCCCCAATTGGGCCGTTAGGCGTTTCCGGTGGAAAGGTCCTANNTTAAGCAGANGCCNCTTCGGGGCTAACC
TANAAGNNCCCCNCTTGGNTTNCCNGGCCTTNTCGGAAATTTNATTTCGGACCNCCCCTTTAAGGGAGNCCCCAAGCTTGTANAAGNNCCCCNCTTGGNTTNCCNGGCCTTNTCGGAAATTTNATTTCGGACCNCCCCTTTAAGGGAGNCCCCAAGCTTG
GGTCCCAAACCTCGGNTTCCCCTAGTTCCGNGCGGCCAATTGTGCTGAAAGCGRCTGCAAGTGAAGCCCTCTGTTACCTGGTCCCAAACCTCGGNTTCCCCTAGTTCCGNGCGGCCAATTGTGCTGAAAGCGRCTGCAAGTGAAGCCCTCTGTTACCT
GTGTCGATCAAGACCTCAACCCCAGAGGAACTTCGTCATCAACATGACTTGCAGGTTTTGCTGGCAGCTTCCTGAAACAGTGTCGATCAAGACCTCAACCCCAGAGGAACTTCGTCATCAACATGACTTGCAGGTTTTGCTGGCAGCTTCCTGAAACA
GACTACGAGTGTTCAAATTCCACCACCTGCATGACCGTGGCTTGCCCTCGGCAGCGCTATTTCGCCAACTGCACCGTGCGGACTACGAGTGTTCAAATTCCACCACCTGCATGACCGTGGCTTGCCCTCGGCAGCGCTATTTCGCCAACTGCACCGTGCG
TGACCACATTCACTGCCTGGGCAACCGGACTTTCCCTAAGCTGCTGTACTGCAACTGGACAGGTGGCTACAAGTGGTCGATGACCACATTCACTGCCTGGGCAACCGGACTTTCCCTAAGCTGCTGTACTGCAACTGGACAGGTGGCTACAAGTGGTCGA
CAGCCCTGGCTCTCAGCATCACCCTTGGGGGGTTTGGAGCCGATCGCTTCTACCTGGGCCAGTGGCGAGAAGGCCTCGGCCAGCCCTGGCTCTCAGCATCACCCTTGGGGGGTTTGGAGCCGATCGCTTCTACCTGGGCCAGTGGCGAGAAGGCCTCGGC
AAGCTCTTCAGCTTTGGCGGCCTGGGAATATGGACCCTAATCGATGTCTTGCTGATTGGAGTTGGCTATGTGGGACCAGCAAGCTCTTCAGCTTTGGCGGCCTGGGAATATGGACCCTAATCGATGTCTTGCTGATTGGAGTTGGCTATGTGGGACCAGC
GGATGGCTCTTTGTACATTTAGCCGAGGTTATGTGCTTCAGAGAGCAGTGTAGAGTCCTGTGTGTGGAGATGGATGCGGTGGATGGCTCTTTGTACATTTAGCCGAGGTTATGTGCTTCAGAGAGCAGTGTAGAGTCCTGTGTGTGGAGATGGATGCGGT
GTGAGAGGGGGATTTATGTTTTTAATGTACAGCATCTGTACTTGGCTTGCCTTGATGAAGGTAAAATAAAGAAATCGAACGTGAGAGGGGGATTTATGTTTTTAATGTACAGCATCTGTACTTGGCTTGCCTTGATGAAGGTAAAATAAAGAAATCGAAC
MCTGAAAAAAAAAAAAAAAAAAAAAAAMCTGAAAAAAAAAAAAAAAAAAAAAAA
Klon 0019-T7-SequenzClone 0019-T7 sequence
CCTGGTAGGAGCCGCAGTGTGTGGCCATGCCTCAGATAGGTTTGGGCGCAGAAGGGTGCTGACCTGGAGCTATCTTCTGGCCTGGTAGGAGCCGCAGTGTGTGGCCATGCCTCAGATAGGTTTGGGCGCAGAAGGGTGCTGACCTGGAGCTATCTTCTGG
TGTCCGTGTCCGGCACAGYAGCTGCCTTCATGCCCACCTTCCCCCTCTACTGCCTGTT SGTTTCCTGCTGGCCTCTGCATGTCCGTGTCCGGCACAGYAGCTGCCTTCATGCCCACCTTCCCCCTCTACTGCCTGTT SGTTTCCTGCTGGCCTCTGCA
GTGGCAGGTGTCATGATGAACACAGCCAGTCTCTTGATGGAGTGGACATCAGCCCAGGGTAGCCCTTTGGTGATGACCTTGTGGCAGGTGTCATGATGAACACAGCCAGTCTCTTGATGGAGTGGACATCAGCCCAGGGTAGCCCTTTGGTGATGACCTT
GAACGCCTTGGGCTTCAGCTTTGGGCAGGTCCTGACAGGTTCTGTGGCCTATGGTGTGCGCAGCTGGAGGATGCTACAGCGAACGCCTTGGGCTTCAGCTTTGGGCAGGTCCTGACAGGTTCTGTGGCCTATGGTGTGCGCAGCTGGAGGATGCTACAGC
TGGCTGTCTCTGCCCCCTTCTTCCTCTTCTTTGTTTATTCATGGTGGCTACCAGAATCGGCACGCTGGCTCATCACGGTATGGCTGTCTCTGCCCCCTTCTTCCTCTTCTTTGTTTATTCATGGTGGCTACCAGAATCGGCACGCTGGCTCATCACGGTA
GGCAAGCTGGACCAGGGCCTGCAGGAACTACAGAGGGTAGCTGCTGTCAACAGGAGGAAGGCAGAGGGAGACACGTTGACGGCAAGCTGGACCAGGGCCTGCAGGAACTACAGAGGGTAGCTGCTGTCAACAGGAGGAAGGCAGAGGGAGACACGTTGAC
CATGGAGGTGCTGCGGTCAGCTATGGAGGAGGAACCAAGCAGGGACAAAGCTGGTGCCAGTCTGGGCACTCTACTCCACACATGGAGGTGCTGCGGTCAGCTATGGAGGAGGAACCAAGCAGGGACAAAGCTGGTGCCAGTCTGGGCACTCTACTCCACA
CACCTGGGCTGCGCCACCGAACCATCATCTCCATGCTGTGCTGGTTTGCCTTTGGCTTCACCTTCTACGGCCTGGCCCTTCACCTGGGCTGCGCCACCGAACCATCATCTCCATGCTGTGCTGGTTTGCCTTTGGCTTCACCTTCTACGGCCTGGCCCTT
GACCTGCAAGCCCTAGGAAGCAATATCTTCCTGCTCCAGGCACTCATCGGGATTGTGGACTTCCCGGTGAAGACAGGCAGGACCTGCAAGCCCTAGGAAGCAATATCTTCCTGCTCCAGGCACTCATCGGGATTGTGGACTTCCCGGTGAAGACAGGCAG
CCTGCTGCTGATCAGCCGCTTGGGCCGGCGCCTCTGCCAGGTCAGCTTCCTGGTGCTCCCCGGACTCTGCATCCTGTCCACCTGCTGCTGATCAGCCGCTTGGGCCGGCGCCTCTGCCAGGTCAGCTTCCTGGTGCTCCCCGGACTCTGCATCCTGTCCA
ACATACTGGTGCCCCATGGGATGGGGGTCCTTCGCTCGGCCCTGGCTGTGCTGGGGCTGGGCTGCCTGGGGGGCGCCTTCACATACTGGTGCCCCATGGGATGGGGGTCCTTCGCTCGGCCCTGGCTGTGCTGGGGCTGGGCTGCCTGGGGGGCGCCTTC
ACCTGCATCACCATCTTTTCCAGTGAGCTCTTTCCCCACTGNGATCAGGGATGACTGCAGNGGGCC Klon 0019-BGH-SequenzACCTGCATCACCATCTTTTCCAGTGAGCTCTTTCCCCACTGNGATCAGGGATGACTGCAGNGGGCC Clone 0019 BGH sequence
TTTTTTTTTTTTTTTTTTTTYTCTTTAAAAATTATTTATTTGKGTGTATCACCGGGGGCGGGTATGTGTCCATGCGGAGC TCAGAGGACAACTTTGTGAAGTCTGTTGTCACATGAGTTTCAGAAÄTTTAGGCCCGGAGGCAGGTGTCTTTACCCGCCGT CGCCAGCCCTGCCCTTTCCTCCTCCTCCTCCTCTTCCTCCTCCTCCTCCTCT CCTCCTCCTTTTTTTTTTTTTTTTTTTTYTCTTTAAAAATTATTTATTTGKGTGTATCACCGGGGGCGGGTATGTGTCCATGCGGAGC TCAGAGGACAACTTTGTGAAGTCTGTTGTCACATGAGTTTCAGAAÄTTTAGGCCCGGAGGCAGGTGTCTTTACCCGCCGT CGCCAGCCCTGCCCTTTCCTCCTCCTCCTCCTCTTCCTCCTCCTCCTCCTCT CCTCCTCC
Klon 0020-ContigClone 0020 contig
GAAAGGGGACGGAGACCTTCAGCGTGGAATCTATATCCAAGAATGGAATCTGTCTGGAGATGGGCCCACAGCCTCAGGGCGAAAGGGGACGGAGACCTTCAGCGTGGAATCTATATCCAAGAATGGAATCTGTCTGGAGATGGGCCCACAGCCTCAGGGC
GTGCTGCGGGCCGACCTGTTCTCCCGGATGCGAGCTCTGGTGGCATCCATTCTGGACTTCATCGAGCTCTTCAACCAAGGGTGCTGCGGGCCGACCTGTTCTCCCGGATGCGAGCTCTGGTGGCATCCATTCTGGACTTCATCGAGCTCTTCAACCAAGG
CATGGACTTACCCGCCTTTGAGATGGATATCTACAGGAACTTGGGCAGTGTGGACTTCCCACGCACTGCGGATGGTGACCCATGGACTTACCCGCCTTTGAGATGGATATCTACAGGAACTTGGGCAGTGTGGACTTCCCACGCACTGCGGATGGTGACC
TGGCTGGCACTGTGCACCCTCAACTGCAGGACCATGACTTTGAGCCACTGAGGCCTGGTGAACCCATCTTCAAGCTTTTCTGGCTGGCACTGTGCACCCTCAACTGCAGGACCATGACTTTGAGCCACTGAGGCCTGGTGAACCCATCTTCAAGCTTTTC
AGCGGAGAAGACGTACTGTATGAGGGGGACTCCATTGTGTACCCTGTGTTCATTAATGAGGCTGCCTATTATGAGAAGCAAGCGGAGAAGACGTACTGTATGAGGGGGACTCCATTGTGTACCCTGTGTTCATTAATGAGGCTGCCTATTATGAGAAGCA
CGTGGCATTCCTGAAGTCTGAGAAGATCAGGGTCACAGTGCCTGCCCTGCTGAGGTTGACCCCCCGCTCCACCCAGACTCCGTGGCATTCCTGAAGTCTGAGAAGATCAGGGTCACAGTGCCTGCCCTGCTGAGGTTGACCCCCCGCTCCACCCAGACTC
CCTAACCCAAATCATACCTGGCCTGATCTCAGTTTTCCTATCTGAACAATGGGTCCCAAGCCCAGGGTCCCCTATCTCACCCTAACCCAAATCATACCTGGCCTGATCTCAGTTTTCCTATCTGAACAATGGGTCCCAAGCCCAGGGTCCCCTATCTCAC
TCTGGACACCATATCCCTTTCCAGACAACTGATCTCATTATATAAAATAGACTGTGCCCCAAAAAAAAAAAAAAATCTGGACACCATATCCCTTTCCAGACAACTGATCTCATTATATAAAATAGACTGTGCCCCAAAAAAAAAAAAAAA
Klon 0022-T7-SequenzClone 0022-T7 sequence
AGCGGACGTTTGTGCCGGGACATGGCCGCTGCGGATGCCGGGTCCTTGAGCTGAGCGCTTGCTGCCGGAGCCAACCTCTGAGCGGACGTTTGTGCCGGGACATGGCCGCTGCGGATGCCGGGTCCTTGAGCTGAGCGCTTGCTGCCGGAGCCAACCTCTG
CCGTCAACCGTCCGCGCGGGCTGGGCCCAGGCGCCGGGACGGCCAAGATCCGCCGAGGAAGCTGAGGCAGCTATAGAACGCCGTCAACCGTCCGCGCGGGCTGGGCCCAGGCGCCGGGACGGCCAAGATCCGCCGAGGAAGCTGAGGCAGCTATAGAACG
CCGCCGCGGCGGGCGCATGGCGTCCATCTTGCTCAGGAGCTGCCGGGGCCGGGGGCCTGCCCGCCTCGCGCCACCTCGGGCCGCCGCGGCGGGCGCATGGCGTCCATCTTGCTCAGGAGCTGCCGGGGCCGGGGGCCTGCCCGCCTCGCGCCACCTCGGG
CCGCCTCCCCGCGGGGTAGTCTGAGGGATCGAGCTTGTCTCAGCTGTACCAGGACCCTGGGGTTGACGAGCCGTGAGAGTCCGCCTCCCCGCGGGGTAGTCTGAGGGATCGAGCTTGTCTCAGCTGTACCAGGACCCTGGGGTTGACGAGCCGTGAGAGT
GTTCTGTCTCGTTGCTGTACTCCAGCCCACCCTGTGTACCTCTGCTTCAAAGGTGAGCCCCTCAGCTGTTGGACTCAGAGGTTCTGTCTCGTTGCTGTACTCCAGCCCACCCTGTGTACCTCTGCTTCAAAGGTGAGCCCCTCAGCTGTTGGACTCAGAG
GCCTGAGTGCCAGGGCACCGCAGCAAGAACAACATGGACACCTGCCTCTGCAAGGCTGGTGGTTACGGGACCTCAGTACCGCCTGAGTGCCAGGGCACCGCAGCAAGAACAACATGGACACCTGCCTCTGCAAGGCTGGTGGTTACGGGACCTCAGTACC
TTCCTGTGCGCGGCTGGCACTCATCATCTYCGCTAGGAGAGGACTCTGTGATAGAGAAGTCCCTTAAGTCCTTAAAAGACTTCCTGTGCGCGGCTGGCACTCATCATCTYCGCTAGGAGAGGACTCTGTGATAGAGAAGTCCCTTAAGTCCTTAAAAGAC
AAGAATAAGAAGCTGGAGGAGGGCGGCCCCGTGTACAGCCCGGCCGCGCAGGTGGCTGGTGAGGAAATCCCTNGGGCAGAAAGAATAAGAAGCTGGAGGAGGGCGGCCCCGTGTACAGCCCGGCCGCGCAGGTGGCTGGTGAGGAAATCCCTNGGGCAGA
AGGTACTGGATGAGCTGAGGCACTACTACCATGGCTTTCCGCCTGCTCTGGGATNGACACCAAAGANCGCTTGAGGTACTGGATGAGCTGAGGCACTACTACCATGGCTTTCCGCCTGCTCTGGGATNGACACCAAAGANCGCTTG
Klon 0022-BGH-SequenzClone 0022 BGH sequence
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATTATGGAAGTTTCTGATTTATTCCAGACAAAATATTAGATTTGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATTATGGAAGTTTCTGATTTATTCCAGACAAAATATTAGATTTGCC
ACTAAGAATCACCTCAAAGCAATCACTCTTGNGTGTCATGCCCCTGNGGCTGGCACGGCATGATGAAGGCAGACCTGNGGACTAAGAATCACCTCAAAGCAATCACTCTTGNGTGTCATGCCCCTGNGGCTGGCACGGCATGATGAAGGCAGACCTGNGG
GCCAGAAGCTGGCAGCTCTAATTCTTCACTTCTGCGGCCTCTTTCTCAGCCTTCTCCTTGGCCTTCTCTTTCTCTTCTATGCCAGAAGCTGGCAGCTCTAATTCTTCACTTCTGCGGCCTCTTTCTCAGCCTTCTCCTTGGCCTTCTCTTTCTCTTCTAT
CTTTTCTTCTTTCTCTAGNGNGGCCACAATTTCAGCTACCTGGGNGGNAGAGATCTGAACATCTTCTTTGTTCACCAAGTCTTTTCTTCTTTCTCTAGNGNGGCCACAATTTCAGCTACCTGGGNGGNAGAGATCTGAACATCTTCTTTGTTCACCAAGT
CGATTACCTTGACAAGGTCATCGATGTTGATATTGCCGNCCTTATTATCATCCAGGGCTGAGGTCAAACTGATGAGCTTGCGATTACCTTGACAAGGTCATCGATGTTGATATTGCCGNCCTTATTATCATCCAGGGCTGAGGTCAAACTGATGAGCTTG
AGCTCTGGAATGNGCTTGATTTGCTTCATGGCGCTGATGAGCTCAGTGATGCTGATGACACTCTCCCCTGTWGGAGNGCTAGCTCTGGAATGNGCTTGATTTGCTTCATGGCGCTGATGAGCTCAGTGATGCTGATGACACTCTCCCCTGTWGGAGNGCT
CTGGCTGGGGCCCAGCTTGCCATCCTGWTGAGAGGWCTCCAGCTGTGTGATCAGGCCATCGATCTGCCCGATCATCTGCTCTGGCTGGGGCCCAGCTTGCCATCCTGWTGAGAGGWCTCCAGCTGTGTGATCAGGCCATCGATCTGCCCGATCATCTGCT
GCACTCGCTTTGACAGCCTCTTGCTCGCTGCARA TCTTCTATGNACTTTCTCCTCACCCGTCTNTTGAAAGKTCCTTCTGCACTCGCTTTGACAGCCTCTTGCTCGCTGCARA TCTTCTATGNACTTTCTCCTCACCCGTCTNTTGAAAGKTCCTTCT
TTGA CTCCTGTAAGTCCTCACNTGTAGGCCTGGACGCCCTCTTTTGAGMRSRSNTCCAGGCTTCCTCCTTTCTCCCTTGTTGA CTCCTGTAAGTCCTCACNTGTAGGCCTGGACGCCCTCTTTTGAGMRSRSNTCCAGGCTTCCTCCTTTCTCCCTTG
GTCAGGGACTTCTTCTGCTNCTGCAGCTTGGAACAGGCGTACTGAGGATGTNAAGTCAGGGACTTCTTCTGCTNCTGCAGCTTGGAACAGGCGTACTGAGGATGTNAA
Klon 0023-T7-SequenzClone 0023-T7 sequence
ATGGACCGCTTCGTGTGGACCAGCGGCCTCCTGGAGATCAACGAGACCCTGGTTATCCAGCAGCGCGGGGTGCGCGTCTAATGGACCGCTTCGTGTGGACCAGCGGCCTCCTGGAGATCAACGAGACCCTGGTTATCCAGCAGCGCGGGGTGCGCGTCTA
CGACGGCGAGGAGAAGATAAAATTTGATGCCGGGACTCTTCTTCTTAGTACACACCGGCTGATTTGGAGAGACCAGAAGACGACGGCGAGGAGAAGATAAAATTTGATGCCGGGACTCTTCTTCTTAGTACACACCGGCTGATTTGGAGAGACCAGAAGA
ATAATGAGTGCTGCATGGCCATTCCCCTGTCTCAGATTGTGTTCATCGAGGAGCAGGCAGCTGGAATCGGGAAGAGCGCCATAATGAGTGCTGCATGGCCATTCCCCTGTCTCAGATTGTGTTCATCGAGGAGCAGGCAGCTGGAATCGGGAAGAGCGCC
AAAATCGTGGTTCACCTGCACCCAGCTCCTTCTAACAAAGAGCCCGGTCCGTTCCAGAGCAGTAAGAACTCCTACATCAGAAAATCGTGGTTCACCTGCACCCAGCTCCTTCTAACAAAGAGCCCGGTCCGTTCCAGAGCAGTAAGAACTCCTACATCAG
ACTCTCCTTCAAAGAACATGGCCAGATTGAGTTTTACAGGCGTTTATCAGAAGAAATGACACAGAGAAGATGGGAGACTGACTCTCCTTCAAAGAACATGGCCAGATTGAGTTTTACAGGCGTTTATCAGAAGAAATGACACAGAGAAGATGGGAGACTG
TGCCAGTTTCCCAGTCACTACAAACAAATAAAGGACCCCAGCCAGGAAGAGTAÄGAGCTGTTGGAATTGTAGGTATTGAATGCCAGTTTCCCAGTCACTACAAACAAATAAAGGACCCCAGCCAGGAAGAGTAÄGAGCTGTTGGAATTGTAGGTATTGAA
AGGAAACTTGAAGAAAAAAGAAAAGAAACCGATAAAAACATTTCTGAGGCCTTTGAAGACCTGAGCAAGCTGATGATTAAAGGAAACTTGAAGAAAAAAGAAAAGAAACCGATAAAAACATTTCTGAGGCCTTTGAAGACCTGAGCAAGCTGATGATTAA
GGCTAAGGAAATGGTGGAGTTATCCAAATCAATCGCTAATAAGATTAAAGAGAAGCAAGGCGATGTCACAGAGGATGAGAGGCTAAGGAAATGGTGGAGTTATCCAAATCAATCGCTAATAAGATTAAAGAGAAGCAAGGCGATGTCACAGAGGATGAGA
CCATCAGGTTTAAGTCGTACCTGCTGAGTATGGGAATAGCCAACCCAGTCACCAGAGAAACCTATGGCTCCGGCACACAGCCATCAGGTTTAAGTCGTACCTGCTGAGTATGGGAATAGCCAACCCAGTCACCAGAGAAACCTATGGCTCCGGCACACAG
TACCACATGCAGCTGGCCAAACAGGCTGGCTGGAATATTGCAGGCACCTTTGGAGGAGCGTGGGGGAATAATGNCCCTCATACCACATGCAGCTGGCCAAACAGGCTGGCTGGAATATTGCAGGCACCTTTGGAGGAGCGTGGGGGAATAATGNCCCTCA
CAGAGGNGCAGAGGNG
Klon 0024-T7-SequenzClone 0024-T7 sequence
AAAGTGGGGATCCTGGWGACAGTTCTGGTGCAGAGTTCCAGTACCTCTACATCCATCATTGTCAGCATGGTCTCCTCCGGAAAGTGGGGATCCTGGWGACAGTTCTGGTGCAGAGTTCCAGTACCTCTACATCCATCATTGTCAGCATGGTCTCCTCCGG
CTTGTTGGAGGTGAGCTCCGCCATTCCGATCATCATGGGCTCCAACATCGGAACCTCTGTCACCAACACCATTGTGGCCCCTTGTTGGAGGTGAGCTCCGCCATTCCGATCATCATGGGCTCCAACATCGGAACCTCTGTCACCAACACCATTGTGGCCC
TGATGCAGGCAGGGGACAGGACTGACTTCAGGCGGGCTTTTGCAGGGGCGACCGTGCATGACTGTTTTAACTGGCTGTCTTGATGCAGGCAGGGGACAGGACTGACTTCAGGCGGGCTTTTGCAGGGGCGACCGTGCATGACTGTTTTAACTGGCTGTCT
GTTCTGGTCCTACTGCCCCTGGAGGCTGCCACGGGCTACCTACACCATGTCACCGGGCTTGAGGTTGCTTCCTTCAACATGTTCTGGTCCTACTGCCCCTGGAGGCTGCCACGGGCTACCTACACCATGTCACCGGGCTTGAGGTTGCTTCCTTCAACAT
CCGAGGTGGCCGTGATGCCCCCGACCTTCTCAAAGTCATCACAGAGCCCTTCACAAGACTCATCATCCAGCTGGACAAGTCCGAGGTGGCCGTGATGCCCCCGACCTTCTCAAAGTCATCACAGAGCCCTTCACAAGACTCATCATCCAGCTGGACAAGT
CTGTGATCACCAGCATTGCCGTGGGGGATGAGTCCCTGAGGAATCACAGTCTCATTCGGATTTGGTGTCACCCAGACACA ACAGAGGCTTCCACTTCTATGTCCAGGGTAGAGGCCATCGGCAGCCTTGCAAACACCACCATGGAGAAATGCAACCATAT CTTCGTGGATACGGGGCTGCCCGACCTGGCCCGTGGGGCTCATTCTGCTGGCGGGCTCCCTGGTGGTTCTCTGCACTTGT CTTATCCTCCTTGNGAAGATGCTCAACTCCCTGCTCAAGGGCCAAGTGGCCAATGTCATCCAGAAGGTCATCAACACAGA CTCTGTGATCACCAGCATTGCCGTGGGGGATGAGTCCCTGAGGAATCACAGTCTCATTCGGATTTGGTGTCACCCAGACACA ACAGAGGCTTCCACTTCTATGTCCAGGGTAGAGGCCATCGGCAGCCTTGCAAACACCACCATGGAGAAATGCAACCATAT CTTCGTGGATACGGGGCTGCCCGACCTGGCCCGTGGGGCTCATTCTGCTGGCGGGCTCCCTGGTGGTTCTCTGCACTTGT CTTATCCTCCTTGNGAAGATGCTCAACTCCCTGCTCAAGGGCCAAGTGGCCAATGTCATCCAGAAGGTCATCAACACAGA CT
Klon 0025-T7-SequenzClone 0025-T7 sequence
AGGGCGCCAGCTGAAGACGCGGGACTTAAAGCGCGTAGCCAGAACCCAGGCACCAGTGTGTCCATTGTCCAGAACTCATCAGGGCGCCAGCTGAAGACGCGGGACTTAAAGCGCGTAGCCAGAACCCAGGCACCAGTGTGTCCATTGTCCAGAACTCATC
TGAAAAACTGCCACAGGAATTGCTTCTCTGCTCCAGGCTGGTCACTGAACAGGTTGCTCCAGGACCTGCAGAATGGGGGCTGAAAAACTGCCACAGGAATTGCTTCTCTGCTCCAGGCTGGTCACTGAACAGGTTGCTCCAGGACCTGCAGAATGGGGGC
AGGCTGTGTCAAAGTCACCAAGTATTTCCTCTTCCTCTTCAACTTGCTGTTCTTTATCCTGGGTGCTGAGATCCTGGGCTAGGCTGTGTCAAAGTCACCAAGTATTTCCTCTTCCTCTTCAACTTGCTGTTCTTTATCCTGGGTGCTGAGATCCTGGGCT
TCGGGGWGTGGATTCTTGCAGACAAGAACAGCTTCATTTCCGTCCTACAAACCTCATCCAGCTCGCTGCAGGTGGGGGCTTCGGGGWGTGGATTCTTGCAGACAAGAACAGCTTCATTTCCGTCCTACAAACCTCATCCAGCTCGCTGCAGGTGGGGGCT
TACGTCTTCATCGG GTGGGCGCCATCACCATAGTGATGGGCTTCCTGGGCTGTATCGG GCTGTCAATGAGGTCCGCTGTACGTCTTCATCGG GTGGGCGCCATCACCATAGTGATGGGCTTCCTGGGCTGTATCGG GCTGTCAATGAGGTCCGCTG
CTTGCTGGGTCTGTACTTTGTCTTCCTTCTGCTGATCCTCATCGCACAGGTGACCGTAGGGGTCCTCTTCTACTTCAACGCTTGCTGGGTCTGTACTTTGTCTTCCTTCTGCTGATCCTCATCGCACAGGTGACCGTAGGGGTCCTCTTCTACTTCAACG
CTGACAAGCTGAAGAAGGAGATGGGGAACACAGTGATGGACATCÄTTCGCAACTACACTGCCAATGCCACCAGTAGCCGCCTGACAAGCTGAAGAAGGAGATGGGGAACACAGTGATGGACATCÄTTCGCAACTACACTGCCAATGCCACCAGTAGCCGC
GAGGAGGCCTGGGACTACGTGCAGGCGCAGGTCAAGTGCTGTGGCTGNGTCAGCCACTACAACTGNACAGAGAACGAGGAGAGGAGGCCTGGGACTACGTGCAGGCGCAGGTCAAGTGCTGTGGCTGNGTCAGCCACTACAACTGNACAGAGAACGAGGA
GCTCATGGGCTTTACCAAGACCACTTACCCATGCTCCTGCGAGAAGGATCAAGGNAAGAGGACAACCAGCTCATTGTGAAGCTCATGGGCTTTACCAAGACCACTTACCCATGCTCCTGCGAGAAGGATCAAGGNAAGAGGACAACCAGCTCATTGTGAA
GAAAGGATTCTTGCGAGGCTGATAACAGCACCTGTGAGCGAAAACAACCCCTGAGGATTGAAAGGATTCTTGCGAGGCTGATAACAGCACCTGTGAGCGAAAACAACCCCTGAGGATT
Klon 0026-T7-SequenzClone 0026-T7 sequence
AGGAGCCCTCGAAAGCGACATGGCGGTTCTCTTAAAGCTGGGCGTTCTCTGCAGTGGCCAAGGAGCTCGAGCTCTCCTACAGGAGCCCTCGAAAGCGACATGGCGGTTCTCTTAAAGCTGGGCGTTCTCTGCAGTGGCCAAGGAGCTCGAGCTCTCCTAC
TCCGAAGCCGGGTGGTCAGACCCGCTTATGTGTCAGCATTTCTCCAGGACCAGCCTACCCAAGGACGG GTGGTACCCAGTCCGAAGCCGGGTGGTCAGACCCGCTTATGTGTCAGCATTTCTCCAGGACCAGCCTACCCAAGGACGG GTGGTACCCAG
CACATTCACCTGTCACCAAGCCACCACTCTGGTTYCAAGGCTGCATCTCTCCACTGGACCAGTGAGAGGG WGTCAGTGTCACATTCACCTGTCACCAAGCCACCACTCTGGTTYCAAGGCTGCATCTCTCCACTGGACCAGTGAGAGGG WGTCAGTGT
TCTGCTCTTGGGGCTGATCCCTGCTGGGTACTTGAATCCCTGCTCTGTGGTGGACTACTCTCTGGCTTCAGCCCTCACCCTCTGCTCTTGGGGCTGATCCCTGCTGGGTACTTGAATCCCTGCTCTGTGGTGGACTACTCTCTGGCTTCAGCCCTCACCC
TGCACAGTCACTGGGGCCTTGGACAAGTGGTTACCGACTACGTTCATGGGGACACCCTGCCGAAGGCTGCCAGGGCAGGCTGCACAGTCACTGGGGCCTTGGACAAGTGGTTACCGACTACGTTCATGGGGACACCCTGCCGAAGGCTGCCAGGGCAGGC
CTCTTGGCACTCTCAGCTTTGACCTTTGCTGGGCTTTGCTACTTCAATTACCACGATGTCGGCATCTGCAGAGCGGTTGCCTCTTGGCACTCTCAGCTTTGACCTTTGCTGGGCTTTGCTACTTCAATTACCACGATGTCGGCATCTGCAGAGCGGTTGC
CATGCTGTGGAAGCTCTGACCTGGGTGCAGCACTTTGATTGTGTGCCTCCTTGCCTCTGCTTTACCAATGCCGTTCACCTCATGCTGTGGAAGCTCTGACCTGGGTGCAGCACTTTGATTGTGTGCCTCCTTGCCTCTGCTTTACCAATGCCGTTCACCT
CGCAGTGAGGGGGGATGAAGGATAAGCCCATTGGTGGGCAGAATGTCTTCTAATTACATGGGTATTTTCAGAATTATTTGCGCAGTGAGGGGGGATGAAGGATAAGCCCATTGGTGGGCAGAATGTCTTCTAATTACATGGGTATTTTCAGAATTATTTG
NTGAGGAAGAGGNTTNTGAGGAAGAGGNTT
Klon 0028-BGH-SequenzClone 0028 BGH sequence
TTTTTTTTTTTTTTTTTCTTTTTTTAAGATAATGGTCTTTTAATTGAGTTATTGAGATGAAGAGACAGTGAAGCCCTGTTTTTTTTTTTTTTTTTTTCTTTTTTTAAGATAATGGTCTTTTAATTGAGTTATTGAGATGAAGAGACAGTGAAGCCCTGTT
TGCTACTTACATGAAAAGAAGATTTTAAAAAACAATCACTGCACAAAATACAAAGGGGCAGGGTATGCTGWGGCATTGAATGCTACTTACATGAAAAGAAGATTTTAAAAAACAATCACTGCACAAAATACAAAGGGGCAGGGTATGCTGWGGCATTGAA
TTCCTCCCCACGTTTTTTCTTGACTTCTCAAGAACAAATTAAAGTCTCCACAGCAAATTTGTTCTCAAAATGCCGAATGGTTCCTCCCCACGTTTTTTCTTGACTTCTCAAGAACAAATTAAAGTCTCCACAGCAAATTTGTTCTCAAAATGCCGAATGG
TGAAACAGTTACTGGCTTCCCGCTTCTGAATACCTCTAATTGTTTCCCGGCGCTGCAGTTTGTAGGTCTCCTTGTCGTGATGAAACAGTTACTGGCTTCCCGCTTCTGAATACCTCTAATTGTTTCCCGGCGCTGCAGTTTGTAGGTCTCCTTGTCGTGA
CACAGTCGGTAGATGAAGAAGCCCAGGTTGTCCACGTTCTCGATGCGGTCGGTGATCACCATGTGCTCATGGATAAGGTACACAGTCGGTAGATGAAGAAGCCCAGGTTGTCCACGTTCTCGATGCGGTCGGTGATCACCATGTGCTCATGGATAAGGTA
GGACTGAGGCAGGTAGGTCCCGGCCTTAATGTTAATAAGGAGCTCCAGCAGGTTTTTGGGCGGCATAACGATGGAAGTGTGGACTGAGGCAGGTAGGTCCCGGCCTTAATGTTAATAAGGAGCTCCAGCAGGTTTTTGGGCGGCATAACGATGGAAGTGT
TCAGAGGAATCACGTAGCACTTGTCCAGGTTAAGGTTCAGAGGAATCACGTAGCACTTGTCCAGGTTAAGGT
Klon 0029-T7-SequenzClone 0029-T7 sequence
AAAGAGCGGCTGCTGTCGGAAGCACCGGGCGAGCTATCTGTTACAGTCCGGCCCGGGGATGGCTCGGGACGCGGAGCTGGAAAGAGCGGCTGCTGTCGGAAGCACCGGGCGAGCTATCTGTTACAGTCCGGCCCGGGGATGGCTCGGGACGCGGAGCTGG
CGCGCAGTAGCGGGTGGCCGTGGCGG GGCTGCCGGCGCTGCTGCTGCTGCAGCTGCTGCGGTGGAGGTGCGCCCTGTGCCGCGCAGTAGCGGGTGGCCGTGGCGG GGCTGCCGGCGCTGCTGCTGCTGCAGCTGCTGCGGTGGAGGTGCGCCCTGTGC
GCGCTCCCCTTCACCAGCAGTCGGCACCCAGGCTTTGCGGACCTGCTGTCGGAGCAGCAGCTGTTGGAGGTGCAGGACTTGCGCTCCCCTTCACCAGCAGTCGGCACCCAGGCTTTGCGGACCTGCTGTCGGAGCAGCAGCTGTTGGAGGTGCAGGACTT
GACCCTGTCTTTGCTGCAGGGCGGAGGTCTAGGGCCGCTGTCACTGCTACCTCCGGACCTGCCGGATCTGGAGCCTGAGTGACCCTGTCTTTGCTGCAGGGCGGAGGTCTAGGGCCGCTGTCACTGCTACCTCCGGACCTGCCGGATCTGGAGCCTGAGT
GCCGGGAGCTGCTGATGGACTTCGCCAATAGCAGCGCCGAGCTGACCGCCTGTATGGTGCGCAGCGCTCGGCCCGTGCGCGCCGGGAGCTGCTGATGGACTTCGCCAATAGCAGCGCCGAGCTGACCGCCTGTATGGTGCGCAGCGCTCGGCCCGTGCGC
CTCTGCCAGACCTGCTACCCGCTCTTCCAACAGGTCGCAATCAAGATGGACAACATCAGCCCGAAACATCGGGAATACCTCTCTGCCAGACCTGCTACCCGCTCTTCCAACAGGTCGCAATCAAGATGGACAACATCAGCCCGAAACATCGGGAATACCT
CCGAGGGCCCGCGCTGAGGCGGAAGTCTCCTGACGGCAGACAGAATGCAGATAGTTCTCATGGTCTCTGAGTTTTTCAACCCGAGGGCCCGCGCTGAGGCGGAAGTCTCCTGACGGCAGACAGAATGCAGATAGTTCTCATGGTCTCTGAGTTTTTCAAC
AGCACGTGGCAGGAGGCGAACTGCGCAAATTGCCTAACAAACAATGGTGAGGATTTGTCAAACAACACAGAGGACTTCCTAGCACGTGGCAGGAGGCGAACTGCGCAAATTGCCTAACAAACAATGGTGAGGATTTGTCAAACAACACAGAGGACTTCCT
CAGTCTGTTTAACAAGACTTTGGCCTGCTTTGAGCATAACCTGCAGGGGCACACATACAGNCTCCTTCCACCAAAAAATTCAGTCTGTTTAACAAGACTTTGGCCTGCTTTGAGCATAACCTGCAGGGGCACACATACAGNCTCCTTCCACCAAAAAATT
ACTCCGAAGTGTGCAGAAACTTGTAAAGAGGCATATAAAAACCTGAGCCTNCTGTACAGTCAAATGNAACTCCGAAGTGTGCAGAAACTTGTAAAGAGGCATATAAAAACCTGAGCCTNCTGTACAGTCAAATGNA
Klon 0029-BGH-SequenzClone 0029 BGH sequence
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTGTTATTTCCTTTATTGGAAAGCATAAGGAAAAAACAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTGTTATTTCCTTTATTGGAAAGCATAAGGAAAAAACAGGTTTT
CTTGDGCACACACATAACCCTATGKGCCTMAGGATTCAGAACTATGTACATTTTTTTAATATGACCACAAGATGAAATTCCTTGDGCACACACATAACCCTATGKGCCTMAGGATTCAGAACTATGTACATTTTTTTAATATGACCACAAGATGAAATTC
TTTGGCACATTTTCAAATATATTTCTAATGCAACCTCTAGAGAGCCAGACCCTGATCAGGAACAGAGATGGCTGGCTTGTTTTGGCACATTTTCAAATATATTTCTAATGCAACCTCTAGAGAGCCAGACCCTGATCAGGAACAGAGATGGCTGGCTTGT
TAAAGGGCTCTCCAGCTTCTTAGCCAAAAGCAGGGGTTTGTACACACAGTACTGAAAGGNACCCGAGGAAGTCGCTACTCTAAAGGGCTCTCCAGCTTCTTAGCCAAAAGCAGGGGTTTGTACACACAGTACTGAAAGGNACCCGAGGAAGTCGCTACTC
ACAGGTTTAAATATGTCACTTCACTCACTTTGCACATGTAAATAAGNTTTACATGTACTGATGAAGATGGNTTCCAATGAACAGGTTTAAATATGTCACTTCACTCACTTTGCACATGTAAATAAGNTTTACATGTACTGATGAAGATGGNTTCCAATGA
CCCTNAACCATGNGCTTCAAATCAAGACAGGAACAATGACAGCNCAATGAACCCCGGCACÄTNTAGGGGATCACAGCGNCCCCTNAACCATGNGCTTCAAATCAAGACAGGAACAATGACAGCNCAATGAACCCCGGCACÄTNTAGGGGATCACAGCGNC
GNCTGATTGTCACATACCCGGGGTGACACACTCTGGGACTAAGACTAGCCTGCTNTCACACTCTGCANATGTGGNAAACAGNCTGATTGTCACATACCCGGGGTGACACACTCTGGGACTAAGACTAGCCTGCTNTCACACTCTGCANATGTGGNAAACA
TACAAAAAATACCCAAACACTCCTGCCTTCCTGTAGGGCAAANACAGGTTTTNAAGG Klon 0030-ContigTACAAAAAATACCCAAACACTCCTGCCTTCCTGTAGGGCAAANACAGGTTTTNAAGG Clone 0030 contig
AAAGGCGCGGAGCCTGCTGCTGCCATGGTGGCTGGTGGCTGGGTACACTATGTCTAGACTGGGGGCCCTGGGCGGCTCCCAAAGGCGCGGAGCCTGCTGCTGCCATGGTGGCTGGTGGCTGGGTACACTATGTCTAGACTGGGGGCCCTGGGCGGCTCCC
GCGCCGGGTTGGGACTGTTACTTGGTACTGCCGCCGGCCTTGGATTCCTGTGCGTCCTTTACAGCCAGCGATGGAAACGGGCGCCGGGTTGGGACTGTTACTTGGTACTGCCGCCGGCCTTGGATTCCTGTGCGTCCTTTACAGCCAGCGATGGAAACGG
ACCCAGCGCCATGGCCGGAGTCACAGTCTGCCCAACTCCCTGGACTATGCGCAGGCTTCAGAGCGTGGACGCCAGGTGACACCCAGCGCCATGGCCGGAGTCACAGTCTGCCCAACTCCCTGGACTATGCGCAGGCTTCAGAGCGTGGACGCCAGGTGAC
ACAGTTTCGGGCTATCCCAGGTGAAGCTGGAGATGCTGCCATACTGCCCAGCCTCTCACAGGAAGGGCAGGAGAAGGTGCACAGTTTCGGGCTATCCCAGGTGAAGCTGGAGATGCTGCCATACTGCCCAGCCTCTCACAGGAAGGGCAGGAGAAGGTGC
TGGACCGCCTGGACTTTGTGCTGACCAGTCTTATGGCGCTGCGGCGCGAGGTGGAGGAGCTTCAGAGAAGCCTGCAAGGATGGACCGCCTGGACTTTGTGCTGACCAGTCTTATGGCGCTGCGGCGCGAGGTGGAGGAGCTTCAGAGAAGCCTGCAAGGA
CTAGCTGGGGAGATTGTCGGGGAGGTCCGCTCTCATATAGÄAGAGAACCAGAGAGTGGCCCGGCGGCGCAGGTTCCCTTTCTAGCTGGGGAGATTGTCGGGGAGGTCCGCTCTCATATAGÄAGAGAACCAGAGAGTGGCCCGGCGGCGCAGGTTCCCTTT
TGCCAGAGAGAGGAGTGACTCCACGGGCTCCAGCTCTGTCTACTTCACCGCCTCCTCAGGGGCCGCACTCACAGACGCCGTGCCAGAGAGAGGAGTGACTCCACGGGCTCCAGCTCTGTCTACTTCACCGCCTCCTCAGGGGCCGCACTCACAGACGCCG
AGAGCGAGGGAGGCTATACAACAGCCAACGCGGAGTCTGATTACGAGCGGGACTCCGACAAGGAGAGTGGAGATGCTGAGAGAGCGAGGGAGGCTATACAACAGCCAACGCGGAGTCTGATTACGAGCGGGACTCCGACAAGGAGAGTGGAGATGCTGAG
GACGAAGTGAGCTGCGAGACCGTGAGGATGGGGAGGAAGGACTCTCTGGACCTGGATGTGGAGGCGGCGTCCAGTCCGTCGACGAAGTGAGCTGCGAGACCGTGAGGATGGGGAGGAAGGACTCTCTGGACCTGGATGTGGAGGCGGCGTCCAGTCCGTC
Klon 0031-T7-SequenzClone 0031-T7 sequence
AGGCTTGCCGTCCAGGCCATGCGGCTGGWGGTCTGCATCG GGCGTTTCCCATGTTTCTGCTGAACCTTCTAGGCATGTGAGGCTTGCCGTCCAGGCCATGCGGCTGGWGGTCTGCATCG GGCGTTTCCCATGTTTCTGCTGAACCTTCTAGGCATGTG
GAGCTGGGTATGCAAAAAGTGCTTTCCCTACTTCCTGAAGCGGTTCGCCATGATATACAATTGGAAGATGGCGAGCCTAAGAGCTGGGTATGCAAAAAGTGCTTTCCCTACTTCCTGAAGCGGTTCGCCATGATATACAATTGGAAGATGGCGAGCCTAA
AGCGGGAGCTCTTCAGCAATCTGCAGGAGTTCGCCGGCCCCTCGGGGAAGCTAACTCTGCTGGAGGTGGGCTGCGGCACCAGCGGGAGCTCTTCAGCAATCTGCAGGAGTTCGCCGGCCCCTCGGGGAAGCTAACTCTGCTGGAGGTGGGCTGCGGCACC
GGGGCCAACTTCAAGTTCTATCCCCCCGGG GCAGGGTCACTTGTATCGACCCYAACCCCAACTTTGAGAAGTTCTTGTTGGGGCCAACTTCAAGTTCTATCCCCCCGGG GCAGGGTCACTTGTATCGACCCYAACCCCAACTTTGAGAAGTTCTTGTT
CAAGAGCGTCGCAGAGAACCGGCAGCTGCAGTTCGAGCGCTTCGTGGTGGCAGCCGGGGAGGACATGCACCAGGTGACCGCAAGAGCGTCGCAGAGAACCGGCAGCTGCAGTTCGAGCGCTTCGTGGTGGCAGCCGGGGAGGACATGCACCAGGTGACCG
ATGGCTCTGTGGACGTGGTGGTCTGCACCCTGGTGCTGTGCTCGGTGAAGAACCAGGAGAAGATTCTGCGCGAGGTGTGCATGGCTCTGTGGACGTGGTGGTCTGCACCCTGGTGCTGTGCTCGGTGAAGAACCAGGAGAAGATTCTGCGCGAGGTGTGC
CGAGTGCTGAAGCCGGGAGGGGCTTTTTACTTCATAGACCATGTGGCAGATGAACGGTCTACCTGGAATTACTTCTGGCACGAGTGCTGAAGCCGGGAGGGGCTTTTTACTTCATAGACCATGTGGCAGATGAACGGTCTACCTGGAATTACTTCTGGCA
GCAGGTCCTGGCTCGTGTGTGGTTCCTTGCTTTTGACGGATGCAATCTGACCAGAGAGAGCTGGAAGGCCATAGAGCAGGGCAGGTCCTGGCTCGTGTGTGGTTCCTTGCTTTTGACGGATGCAATCTGACCAGAGAGAGCTGGAAGGCCATAGAGCAGG
CCAACTTCTCCAAGCTGAACCTGCAGCACATNCAGGCCCCGCTCCCCTTGACCTTGGTGCGGNCCCACATCTATGGCTATCCAACTTCTCCAAGCTGAACCTGCAGCACATNCAGGCCCCGCTCCCCTTGACCTTGGTGCGGNCCCACATCTATGGCTAT
GCTGTGAAATAGAGTGCTGTGAAATAGAGT
Klon 0031-BGH-SequenzClone 0031 BGH sequence
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAATATAGAAACCATTTATCAAATGAATATAAATGTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAATATAGAAACCATTTATCAAATGAATATAAATGTATT
GATCAACATTTAAAATATAACTTCTGCAAAATCATCTTGAAAAATATACATTTGTTTAGATCCATACATACAAATGCAGCGATCAACATTTAAAATATAACTTCTGCAAAATCATCTTGAAAAATATACATTTGTTTAGATCCATACATACAAATGCAGC
TGAAACCCTTGGGCCCACCCAGACTTGCTCTCTGTATGAACACAATGATATCCATGGTTTTGTTTCAGGACCAGKGGAATTGAAACCCTTGGGCCCACCCAGACTTGCTCTCTGTATGAACACAATGATATCCATGGTTTTGTTTCAGGACCAGKGGAAT
TTTTCTTCTTCTTCAATACAGGGTTTATTTGTGTAGCCCTGGCGGKCCTGGAACTCCATCTGTAGATCAGGCTGTACTCATTTTCTTCTTCTTCAATACAGGGTTTATTTGTGTAGCCCTGGCGGKCCTGGAACTCCATCTGTAGATCAGGCTGTACTCA
CAGAGATCCACCTACCTCTGCCACCTGAGAGCTGGGATTGAGGCTCTGCCACCAACCACTCAGGACCAGTGATATTTGACCAGAGATCCACCTACCTCTGCCACCTGAGAGCTGGGATTGAGGCTCTGCCACCAACCACTCAGGACCAGTGATATTTGAC
CAGAAGAATCCCTCCCCCCCCGSACCCCGAGTCCTTGGTAACTACTCTCGGGCATTACTTTTAGGGKCCCCTACATACTGCAGAAGAATCCCTCCCCCCCCGSACCCCGAGTCCTTGGTAACTACTCTCGGGCATTACTTTTAGGGKCCCCTACATACTG
GACCCATTTCCCTAACTATAGNGGCCTTCTACTGCCTACGGTAATTATGACCCATTTCCCTAACTATAGNGGCCTTCTACTGCCTACGGTAATTAT
Klon 0032-ContigClone 0032 contig
AAAGAAGGAACTAAACATGGGCCAGCGATGCTCTGACACCAGAGGAATTGCTTTCGAAGACGTCAGAGTGCCTAAGGAAAAAAGAAGGAACTAAACATGGGCCAGCGATGCTCTGACACCAGAGGAATTGCTTTCGAAGACGTCAGAGTGCCTAAGGAAA
ATGTGTTAATCGGTGAAGGAGCAGKTTTCAAGATCGCAATGGGTGCTTTTGATAGAACCAGACCTACAGTCGCAGCTGGCATGTGTTAATCGGTGAAGGAGCAGKTTTCAAGATCGCAATGGGTGCTTTTGATAGAACCAGACCTACAGTCGCAGCTGGC
GCTGTCGGGCTAGCCCAGAGAGCTCTGGACGAAGCCACGAAGTATGCCCTGGATAGGAAGACATTTGGAAAGCTGCTAGTGCTGTCGGGCTAGCCCAGAGAGCTCTGGACGAAGCCACGAAGTATGCCCTGGATAGGAAGACATTTGGAAAGCTGCTAGT
GGAGCACCAAGGAGTTTCATTTCTGCTCGCAGAAATGGCGATGAAGGTTGAACTCGCTAGGCTCAGTTACCAGAGAGCAGGGAGCACCAAGGAGTTTCATTTCTGCTCGCAGAAATGGCGATGAAGGTTGAACTCGCTAGGCTCAGTTACCAGAGAGCAG
CCTGGGAGGTTGACTCCGGTCGCCGGAACACTTACTATGCCTCGATTGCAAAGGCCTTTGCTGGAGACATTGCCAATCAGCCTGGGAGGTTGACTCCGGTCGCCGGAACACTTACTATGCCTCGATTGCAAAGGCCTTTGCTGGAGACATTGCCAATCAG
CTAGCCACTGACGCCGTGCAGATTTTCGGAGGCTATGGATTCAACACAGAGTACCCTGTGGAGAAGCTGATGAGGGACGCCTAGCCACTGACGCCGTGCAGATTTTCGGAGGCTATGGATTCAACACAGAGTACCCTGTGGAGAAGCTGATGAGGGACGC
CAAGATCTATCAGATTTATGAAGGTACTGCACAAATTCAGAGGCTGATCATAGCTCGTGAGCACATTGAAAAGTATAAAACAAGATCTATCAGATTTATGAAGGTACTGCACAAATTCAGAGGCTGATCATAGCTCGTGAGCACATTGAAAAGTATAAAA
ATTAACAGGAATTACTATTGAACGATGCATCACCCTCGTGTAACTAAGCTCCAAGCACTGTTGCTGCTTCAGGGGAAAAGATTAACAGGAATTACTATTGAACGATGCATCACCCTCGTGTAACTAAGCTCCAAGCACTGTTGCTGCTTCAGGGGAAAAG
GGCTTTACTGTCTTCCCAAGGAAATGAGATAAAAGACGAGTTTGGATCTATGCGGCGGATTCCCATGGCGGAGGAACCTGGGCTTTACTGTCTTCCCAAGGAAATGAGATAAAAGACGAGTTTGGATCTATGCGGCGGATTCCCATGGCGGAGGAACCTG
TCTTCAGCTCTATGGTGACCCTTTCTAGATAGGTTTGGCTTTTGGACAATGATTGGTCCTTAGCCCCGAATTGTGTTAGTTCTTCAGCTCTATGGTGACCCTTTCTAGATAGGTTTGGCTTTTGGACAATGATTGGTCCTTAGCCCCGAATTGTGTTAGT
TTGCTCTTTGATCACTTAAAATGGAAAAACACCCTGGACTTTTAATGTTCATTCAAGTGACAGGAAAGGCGGCTTGTCAATTGCTCTTTGATCACTTAAAATGGAAAAACACCCTGGACTTTTAATGTTCATTCAAGTGACAGGAAAGGCGGCTTGTCAA
GGAAGAACTCATGATTCTAACATAAACACTGAAAATTTGTGGTAGATTGGACACGTCAGACTGTGACATAGCAGCATTTCGGAAGAACTCATGATTCTAACATAAACACTGAAAATTTGTGGTAGATTGGACACGTCAGACTGTGACATAGCAGCATTTC
TGTGCTGAACTGTTAATTTTATAATTTTGATTATATTTGCTTTGTTTTGCACAAAAGAGTAAAAAGTTTATATTCATATTTGTGCTGAACTGTTAATTTTATAATTTTGATTATATTTGCTTTGTTTTGCACAAAAGAGTAAAAAGTTTATATTCATATT
CTCCCATTATAAAACTAAAACTTTTCTGGGAATCTTAGTACTGAACGAGAATTTTATTTGTCTTGTTTAAAATAATTCAACTCCCATTATAAAACTAAAACTTTTCTGGGAATCTTAGTACTGAACGAGAATTTTATTTGTCTTGTTTAAAATAATTCAA
TAAAGCTAACCTTAACTTAAAAAAAAAAAAAAAAAAAAAAAAATAAAGCTAACCTTAACTTAAAAAAAAAAAAAAAAAAAAAAAAA
Klon 0033-ContigClone 0033 contig
AGCGTCGCCATCCGCCACCATGGTGAACTTCACAGTÄGATCAGATCCGTGCCATCATGGACAAGAAAGCCAACATCGATAAGCGTCGCCATCCGCCACCATGGTGAACTTCACAGTÄGATCAGATCCGTGCCATCATGGACAAGAAAGCCAACATCGATA
AGGGTGAGGTGTCTGCCCGCCAGGAGCTCAAGGCACGTGCCCGCTACCTGGCCGAAAAGTATGAGTGGGACGTTGCTGAAAGGGTGAGGTGTCTGCCCGCCAGGAGCTCAAGGCACGTGCCCGCTACCTGGCCGAAAAGTATGAGTGGGACGTTGCTGAA
GCCCGCAAGATCTGGTGCTTTGGCCCTGATGGCACTGGCCCCAACATTCTCACCGACATCACCAAGGGTGTGCAGTACCTGCCCGCAAGATCTGGTGCTTTGGCCCTGATGGCACTGGCCCCAACATTCTCACCGACATCACCAAGGGTGTGCAGTACCT
GAATGAGATCAAGGACAGTGTGGTGGCTGGCTTCCAGTGGGCTACTAAGGAGGGCGCTCTCTGTGAGGAAAACATGCGTGGAATGAGATCAAGGACAGTGTGGTGGCTGGCTTCCAGTGGGCTACTAAGGAGGGCGCTCTCTGTGAGGAAAACATGCGTG
GTGTGCGGTTTGATGTTCATGATGTGACCCTGCATGCTGATGCCATTCACCGGGGAGGTGGCCAGATCATCCCCACAGCAGTGTGCGGTTTGATGTTCATGATGTGACCCTGCATGCTGATGCCATTCACCGGGGAGGTGGCCAGATCATCCCCACAGCA
CGCCGCTGCCTGTATGCCAGTGTGCTGACCGCACAGCCCCGCCTCATGGAGCCTATCTATCTGGTGGAGATCCAGTGTCCCGCCGCTGCCTGTATGCCAGTGTGCTGACCGCACAGCCCCGCCTCATGGAGCCTATCTATCTGGTGGAGATCCAGTGTCC
TGAGCAAGTGGTGGGTGGCATCTACGGTGTCCTGAACAGGAAGCGTGGCCATGTGTTTGAGGAGTCCCAGGTGGCTGGTATGAGCAAGTGGTGGGTGGCATCTACGGTGTCCTGAACAGGAAGCGTGGCCATGTGTTTGAGGAGTCCCAGGTGGCTGGTA
CCCCCATGTTTGTGGTCAAGGCATACCTGCCTGTCAATGAGTCCTTTGGCTTCACCGCTGATCTGCGATCCAACACCGGCCCCCCATGTTTGTGGTCAAGGCATACCTGCCTGTCAATGAGTCCTTTGGCTTCACCGCTGATCTGCGATCCAACACCGGC
GGCCAGGCCTTCCCCCAGTGCGTGTTTGACCACTGGCAGATCCTGCCTGGGGATCCTTTTGACAACAGCAGCCGCCCCAG CCAAGTGGTAGNTGAGACGCGCAAGCGCAAGGGCCTGAAAGAGGGCATCCCAGCGCTGGACAACTTCCTGGACAAACTGT AGGCAGCNTGATACTGCCACATGTTGCACAGTGCCCACCCATCAGAAGACCCCTTGAGACTGTCCCACAGTGCTCCTNTA GAGGCTGCTGGGGCCACCCTGACATCACTCAGCACTCACTTGCTACCAATTCTATTTATTTCGGAATT CAAGATΆGCGG GAATCTCTCTGCAGGCTGGACTGGCAGGCTGTGGGGTGGGCGGGACMCGGCTCTTAMCATTTTCARAGGGAAACGCGCAG ATGTCCAAAAGTYTAAATAAATGCATTCAGAGGTTTTTGGGGTCCATGGCCAAGTGGAGTTCCCCCARAGGGGGAGGTGG GGTAAGTGCCTCCAGGAAGGCAGGCAGCCTGCCTTAGMCTTGCAGCCCGGCTGTGGGAATGAAYCATTGGAGTAATAAAC TMCAGTGGTTGATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAΆAAAAAAAAAAAAAGGCCAGGCCTTCCCCCAGTGCGTGTTTGACCACTGGCAGATCCTGCCTGGGGATCCTTTTGACAACAGCAGCCGCCCCAG CCAAGTGGTAGNTGAGACGCGCAAGCGCAAGGGCCTGAAAGAGGGCATCCCAGCGCTGGACAACTTCCTGGACAAACTGT AGGCAGCNTGATACTGCCACATGTTGCACAGTGCCCACCCATCAGAAGACCCCTTGAGACTGTCCCACAGTGCTCCTNTA GAGGCTGCTGGGGCCACCCTGACATCACTCAGCACTCACTTGCTACCAATTCTATTTATTTCGGAATT CAAGATΆGCGG GAATCTCTCTGCAGGCTGGACTGGCAGGCTGTGGGGTGGGCGGGACMCGGCTCTTAMCATTTTCARAGGGAAACGCGCAG ATGTCCAAAAGTYTAAATAAATGCATTCAGAGGTTTTTGGGGTCCATGGCCAAGTGGAGTTCCCCCARAGGGGGAGGTGG GGTAAGTGCCTCCAGGAAGGCAGGCAGCCTGCCTTAGMCTTGCAGCCCGGCTGTGGGAATGAAYCATTGGAGTAATAAAC TMCAGTGGTTGATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAΆAAAAAAAAAAAAA
Klon 0034-T7-SequenzClone 0034-T7 sequence
AAAGGCCAGCTCCTGCTCTGCTCCTCTCCCGCCTGCCGCCGCGCTGCACGCCTCGAGCACTCCCTCGGCCCCGGCGGGGA CCGGGGACCCCGCAGCTACCGCCΆTGCTGCCAGTGCTCTACACCGGCCTGGCGGGGCTGCTGCTGCTGCCTCTGCTGCTC ACCTGCTGCTGCCCCTACCTCCTCCAΆGATGTGCGGTACTTCCTGCGGCTGGCCAACATGGCCCGGCGGGTGCGCAGCTA CCGGCAGCGGCGACCCGTGCGTACCATCCTGCGGGCCTTCCTGGAACAΆGCGCGCAAGACCCCACACAAGCCCTTCCTGC TGTTCCGAGACGAGACGCTCACCTACGCCCAGGWGGACCGGCGCAGCAACCAAGTGGCGCGGGCGCTGCACGATCAACTG GGCCTACGACAGGGGGATTGCGTAGCCCTCTTCATGGGCAATGAGCCGGCCTACGTGTGGATCTGGCTGGGACTGCTCAA ACTGGGCTGTCCCATGGCGTGCCTCAACTACAACATTCGTGCCAAGTCTCTGCTGCACTGCTTTCAATGCTGCGGGGCGA AGGTGCTGCTGGCCTCCCCAGATCTACAAGAAGCTGTGGAGGAGGTTCTTCCAACCCTGAAAAAGGATGCCGTGTCCGTC TTTTACGTAAGCAGAACTTCTAACACAAATGGTGTGGACACÄATACTGGACAAAGTAGACGGAGTGTCGGCGGAACCCAC CCCGGAGTCAAAGGCCAGCTCCTGCTCTGCTCCTCTCCCGCCTGCCGCCGCGCTGCACGCCTCGAGCACTCCCTCGGCCCCGGCGGGGA CCGGGGACCCCGCAGCTACCGCCΆTGCTGCCAGTGCTCTACACCGGCCTGGCGGGGCTGCTGCTGCTGCCTCTGCTGCTC ACCTGCTGCTGCCCCTACCTCCTCCAΆGATGTGCGGTACTTCCTGCGGCTGGCCAACATGGCCCGGCGGGTGCGCAGCTA CCGGCAGCGGCGACCCGTGCGTACCATCCTGCGGGCCTTCCTGGAACAΆGCGCGCAAGACCCCACACAAGCCCTTCCTGC TGTTCCGAGACGAGACGCTCACCTACGCCCAGGWGGACCGGCGCAGCAACCAAGTGGCGCGGGCGCTGCACGATCAACTG GGCCTACGACAGGGGGATTGCGTAGCCCTCTTCATGGGCAATGAGCCGGCCTACGTGTGGATCTGGCTGGGACTGCTCAA ACTGGGCTGTCCCATGGCGTGCCTCAACTACAACATTCGTGCCAAGTCTCTGCTGCACTGCTTTCAATGCTGCGGGGCGA AGGTGCTGCTGGCCTCCCCAGATCTACAAGAAGCTGTGGAGGAGGTTCTTCCAACCCTGAAAAAGGATGCCGTGTCCGTC TTTTACGTAAGCAGAACTTCTAACACAAATGGTGTGGACACÄATACTGGACAAAGTAGACGGAGTGTCGGCGGAACCCAC CCCGGAGTC
Klon 0034-BGH-SequenzClone 0034 BGH sequence
CTTTTTTTTTTTTTTTTTTTTAGTGTTAATATAGTTTATTATGTCTTTAAAAACTTTTTTTTTTTTTTTTTTTTAGTGTTAATATAGTTTATTATGTCTTTAAAAA
GTTTGCAAGGACAAATGGCAGGTGCACATTGAAAAATAATTGTTTCTAAATCTTTTTACTTGCAAAGGTTCAGGTGTAAT TTAAAAAAAAAACAAACAAACTATCCTTTTAATGAATAATTTCCTAAAAATAAAATCGCACCTTATAGCCTTCAATCAAG TTAAAGTTGGATTCTACGTATGAAGTGGCTCTGCGAGGTCTATCGAGTTTCTTTCTGGAAATGTCATGAGCTAAACCACC AGGGAATATTCAGAGCTTCAGAGTTTTATCAATTATGGCATTATAAATGTTCTCAGTCATGGGCACAAATGTTTTCTCTG CATCATCCATGAAATACAAGGTATCTTTGATGACTGTGGGATTGAAGCCCTCTTCCATCAGGGTCACTTTGCGGTGTTTA AAAGTCCCAGTGATCTCAATGGTATCTTGTATCCTCAGGAACCGAGGCCTCGCGTAACTGGGCAGGTACTCCGCGATGTG TTGAAAGAGTTTCTTTCCATTGAACTCGTAGTTTTCTTTGATCTTGAGGGAGGCCATCCCAATTCGACCCTCATGACCTG GCACAGGCACGCCATACACATTCACTTCTTCAACAAAATCTACCAGTCCCACGATGTCAGCGACCTTCTGNGGTAGCTAC GNTCTCTTCTTTTCACCGGAAAGTATCTNCAACCCTGGTTTGCAAGGACAAATGGCAGGTGCACATTGAAAAATAATTGTTTCTAAATCTTTTTACTTGCAAAGGTTCAGGTGTAAT TTAAAAAAAAAACAAACAAACTATCCTTTTAATGAATAATTTCCTAAAAATAAAATCGCACCTTATAGCCTTCAATCAAG TTAAAGTTGGATTCTACGTATGAAGTGGCTCTGCGAGGTCTATCGAGTTTCTTTCTGGAAATGTCATGAGCTAAACCACC AGGGAATATTCAGAGCTTCAGAGTTTTATCAATTATGGCATTATAAATGTTCTCAGTCATGGGCACAAATGTTTTCTCTG CATCATCCATGAAATACAAGGTATCTTTGATGACTGTGGGATTGAAGCCCTCTTCCATCAGGGTCACTTTGCGGTGTTTA AAAGTCCCAGTGATCTCAATGGTATCTTGTATCCTCAGGAACCGAGGCCTCGCGTAACTGGGCAGGTACTCCGCGATGTG TTGAAAGAGTTTCTTTCCATTGAACTCGTAGTTTTCTTTGATCTTGAGGGAGGCCATCCCAATTCGACCCTCATGACCTG GCACAGGCACGCCATACACATTCACTTCTTCAACAAAATCTACCAGTCCCACGATGTCAGCGACCTTCTGNGGTAGCTAC GNTCTCTTCTTTTCACCGGAAAGTATCTNCAACCCTG
Klon 0036-ContigClone 0036 contig
AAAAGGTGGAATCTATATCCAAGAATGGAATCTGTCTGGAGATGGGCCCRCAGCCTCAGGGCGTGCTGCGGGCCGACCTG TTCTCCCGGATGCGAGCTCTGGTGGCATCCATTCTGGACTTCATCGAGCTCTTCAACCAAGGCATGGACΓTACCCGCCTT TGAGATGGATATCTACAGGAACTTGGGCAGTGTGGACTTCCCACGCACTGCGGATGGTGACCTGGCTGGC CTGTGCACC CTCAACTGCAGGACCATGACTTTGAGCCACTGAGGCCTGGTGAACCCATCTTCAAGCTTTTCAGCGGAGAAGACGTACTG TATGAGGGGGACTCCATTGTGTACCCTGTGTTCATTAATGAGGCTGCCTATTATGAGAAGCACGTGGC TTCCTGAAGTC TGAGAAGATCAGGGTCACAGTGCCTGCCCTGCTGAGGTTGACCCCCCGCTCCACCCAGACTCCCTAACCCAAATCATACC TGGCCTGATCTCAGTTTTCCTATCTGAACAATGGGTCCCAAGCCCAGGGTCCCCTATCTCACTCTGGACACCATATCCCT TTCCAGACAACTGATCTCATTATATAAAATAGACTGTGCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAGGTGGAATCTATATCCAAGAATGGAATCTGTCTGGAGATGGGCCCRCAGCCTCAGGGCGTGCTGCGGGCCGACCTG TTCTCCCGGATGCGAGCTCTGGTGGCATCCATTCTGGACTTCATCGAGCTCTTCAACCAAGGCATGGACΓTACCCGCCTT TGAGATGGATATCTACAGGAACTTGGGCAGTGTGGACTTCCCACGCACTGCGGATGGTGACCTGGCTGGC CTGTGCACC CTCAACTGCAGGACCATGACTTTGAGCCACTGAGGCCTGGTGAACCCATCTTCAAGCTTTTCAGCGGAGAAGACGTACTG TATGAGGGGGACTCCATTGTGTACCCTGTGTTCATTAATGAGGCTGCCTATTATGAGAAGCACGTGGC TTCCTGAAGTC TGAGAAGATCAGGGTCACAGTGCCTGCCCTGCTGAGGTTGACCCCCCGCTCCACCCAGACTCCCTAACCCAAATCATACC TGGCCTGATCTCAGTTTTCCTATCTGAACAATGGGTCCCAAGCCCAGGGTCCCCTATCTCACTCTGGACACCATATCCCT TTCCAGACAACTGATCTCATTATATAAAATAGACTGTGCCCCAAAAAAAAAAAAAAAAAAAAAA
Klon 0037-T7-SequenzClone 0037-T7 sequence
AGCTGCATCGTGGCGTTTCCCATGTTTCTGCTGAACCTTCTAGGCATGTGGAGCTGGGTATGCAAAAAGTGCTTTCCCTAAGCTGCATCGTGGCGTTTCCCATGTTTCTGCTGAACCTTCTAGGCATGTGGAGCTGGGTATGCAAAAAGTGCTTTCCCTA
CTTCCTGAAGCGGTTCGCCATGATATACAATTGGAAGATGGCGAGCCTAAAGCGGGAGCTCTTCAGCAATCTGCAGGAGTCTTCCTGAAGCGGTTCGCCATGATATACAATTGGAAGATGGCGAGCCTAAAGCGGGAGCTCTTCAGCAATCTGCAGGAGT
TCGCCGGCCCCTCGGGGAAGCTAACTCTGCTGGAGGTGGGCTGCGGCACCGGGGCCAACTTCAAGTTCTATCCCCCCGGGTCGCCGGCCCCTCGGGGAAGCTAACTCTGCTGGAGGTGGGCTGCGGCACCGGGGCCAACTTCAAGTTCTATCCCCCCGGG
TGCAGGGTCACTTGTATCGACCCCAACCCCAACTTTGAGAAGTTCTTGTTCAAGAGCGTCGCAGAGAACCGGCAGCTGCATGCAGGGTCACTTGTATCGACCCCAACCCCAACTTTGAGAAGTTCTTGTTCAAGAGCGTCGCAGAGAACCGGCAGCTGCA
GTTCGAGCGCTTCGTGGTGGCAGCCGGGGAGGACATGCACCAGGTGACCGATGGCTCTGTGGACGTGGTGGTCTGCACCCGTTCGAGCGCTTCGTGGTGGCAGCCGGGGAGGACATGCACCAGGTGACCGATGGCTCTGTGGACGTGGTGGTCTGCACCC
TGGTGCTGTGCTCGGTGAAGAACCAGGAGAAGATTCTGCGCGAGGTGTGCCGAGTGCTGAAGCCGGGAGGGGCTTTTTACTGGTGCTGTGCTCGGTGAAGAACCAGGAGAAGATTCTGCGCGAGGTGTGCCGAGTGCTGAAGCCGGGAGGGGCTTTTTAC
TTCATAGACCATGTGGCAGATGAACGGTCTACCTGGAATTACTTCTGGCAGCAGGTCCTGGCTCGTGTGTGGTTCCTTGCTTCATAGACCATGTGGCAGATGAACGGTCTACCTGGAATTACTTCTGGCAGCAGGTCCTGGCTCGTGTGTGGTTCCTTGC
TTTTGACGGATGCAATCTGACCAGAGAGAGCTGGAAGGCCATAGAGCAGGCCAACTTCTCCAAGCTGAACCTGCAGCACATTTTGACGGATGCAATCTGACCAGAGAGAGCTGGAAGGCCATAGAGCAGGCCAACTTCTCCAAGCTGAACCTGCAGCACA
TNCAGGCCCCGCTCCCCTTGACCTTGGTGCGGGCCCACATCTATGGCTATGCTGNGAATNCAGGCCCCGCTCCCCTTGACCTTGGTGCGGGCCCACATCTATGGCTATGCTGNGAA
Klon 0037-BGH-SequenzClone 0037 BGH sequence
TTTTTTTTTTTTTTTTTTTTTTTGGCAAATATAGAAACCATTTATCAAATGAATATAAATGTATTGATCAACATTTAAAA TATAACTTCTGCAAAATCATCTTGAAAAATATACATTTGTTTAGATCCATACATACAAATGCAGCTGAAACCCTTGGGCC CACCCAGACTTGCTCTCTGTATGAACACAATGATATCCATGGTTTTGTTTCAGGACCAGTGGAATTTTTCTTCTTCTTCA ATACAGGGTTTATTTGTGTAGCCCTGGCGGTCCTGGAACTCCATCTGTAGATCAGGCTGTACTCACAGAGATCCACCTAC CTCTGCCACCTGAGAGCTGGGATTGAGGCTCTGCCACCAACCACTCAGGACCAGTGATATTTGACCAGAAGAATTTTTTTTTTTTTTTTTTTTTTTTGGCAAATATAGAAACCATTTATCAAATGAATATAAATGTATTGATCAACATTTAAAA TATAACTTCTGCAAAATCATCTTGAAAAATATACATTTGTTTAGATCCATACATACAAATGCAGCTGAAACCCTTGGGCC CACCCAGACTTGCTCTCTGTATGAACACAATGATATCCATGGTTTTGTTTCAGGACCAGTGGAATTTTTCTTCTTCTTCA ATACAGGGTTTATTTGTGTAGCCCTGGCGGTCCTGGAACTCCATCTGTAGATCAGGCTGTACTCACAGAGATCCACCTAC CTCTGCCACCTGAGAGCTGGGATTGAGGCTCTGCCACCAACCACTCAGGACCAGTGATATTTGACCAGAAGAAT
Klon 0038-ContιgClone 0038-Contιg
AAAGGTGGAGCTGGGTGGTGTTTGGTGCGGTACGGCGGCCACTCAGTTGCAGCAGAGCAGGTGCCATCCTGTGGAAGAACAAAGGTGGAGCTGGGTGGTGTTTGGTGCGGTACGGCGGCCACTCAGTTGCAGCAGAGCAGGTGCCATCCTGTGGAAGAAC
CATGAAGCACTACGAGGTGGAGATTCGGGATGCAAAGACGAGGGAGAAGCTGTGCTTCCTGGACAAGGTAGAGCCTCAGGCATGAAGCACTACGAGGTGGAGATTCGGGATGCAAAGACGAGGGAGAAGCTGTGCTTCCTGGACAAGGTAGAGCCTCAGG
CCACCATTTCTGAAATCAAGACCCTTTTCACCAAGACACACCCGCAGTGGTATCCTGCCCGCCAGTCCCTCCGCCTGGACCCACCATTTCTGAAATCAAGACCCTTTTCACCAAGACACACCCGCAGTGGTATCCTGCCCGCCAGTCCCTCCGCCTGGAC
CCCAAGGGGAAGTCCCTGAAAGATGAAGATGTCTTACAGAAGCTTCCTGTGGGCACCACAGCCACACTCTACTTCCGGGACCCAAGGGGAAGTCCCTGAAAGATGAAGATGTCTTACAGAAGCTTCCTGTGGGCACCACAGCCACACTCTACTTCCGGGA
CCTCGGGGCCCAGATCAGCTGGGTGACGGTCTTCCTGACGGAGTATGCCGGGCCCCTTTTCATCTACCTGCTCTTCTACTCCTCGGGGCCCAGATCAGCTGGGTGACGGTCTTCCTGACGGAGTATGCCGGGCCCCTTTTCATCTACCTGCTCTTCTACT
TCCGGGTACCCTTCATTTATGGCCGCAAATACGACTTTACGTCCAGTCGGCATACGGTGGTGCACCTCGCCTGCATGTGCTCCGGGTACCCTTCATTTATGGCCGCAAATACGACTTTACGTCCAGTCGGCATACGGTGGTGCACCTCGCCTGCATGTGC
CACTCGTTCCACTACATCAAGCGCCTGCTGGAGACTCTCTTCGTGCACCGATTCTCTCACGGAACCATGCCTTTGCGAAACACTCGTTCCACTACATCAAGCGCCTGCTGGAGACTCTCTTCGTGCACCGATTCTCTCACGGAACCATGCCTTTGCGAAA
CATCTTCAAAAACTGCACCTACTATTGGGGCTTTGCTGCATGGATGGCTTATTACATCAACCACCCTCTCTACACACCCCCATCTTCAAAAACTGCACCTACTATTGGGGCTTTGCTGCATGGATGGCTTATTACATCAACCACCCTCTCTACACACCCC
CTACCTATGGAGTTCAGCAGGTTAAGCTGGCACTGGCCGTTTTTGTGATCTGCCAGCTTGGGAACTTCTCCATCCACATGCTACCTATGGAGTTCAGCAGGTTAAGCTGGCACTGGCCGTTTTTGTGATCTGCCAGCTTGGGAACTTCTCCATCCACATG
GCTCTTCGGGACCTTCGGCCTGCTGGGTCGAAAACCAGGAAGATCCCATACCCCACCAAGAACCCCTTCACCTGGCTGKTGCTCTTCGGGACCTTCGGCCTGCTGGGTCGAAAACCAGGAAGATCCCATACCCCACCAAGAACCCCTTCACCTGGCTGKT
CCTGTTGGTGTCCTGTCCCAACTACACTTATGAGGTGGGCTCCTGGATTGGCTTTGCSATCTTGACTCAGTGTGTCCCAGCCTGTTGGTGTCCTGTCCCAACTACACTTATGAGGTGGGCTCCTGGATTGGCTTTGCSATCTTGACTCAGTGTGTCCCAG
TGGCCCTCTTCTCCCTGGTGGGCTTCACCCAGATGACTATCTGGGCCAAGGGCAAACACCGCAGCTACCTGAAGGAGTTCTGGCCCTCTTCTCCCTGGTGGGCTTCACCCAGATGACTATCTGGGCCAAGGGCAAACACCGCAGCTACCTGAAGGAGTTC
CGCGACTACCCGCCCCTGCGCATGCCCATTATTCCCTTCCTGCTCTGAGCAGCTCCTCACGGCTCTGCCCAGTAATACTCCGCGACTACCCGCCCCTGCGCATGCCCATTATTCCCTTCCTGCTCTGAGCAGCTCCTCACGGCTCTGCCCAGTAATACTC
TCCACCCCTCACTGCCCCTGTCCTGATGTGTGGCTGGCCATGGCTCTCCAGCAGCAACAATAAAACCTGCTTACCCAAAATCCACCCCTCACTGCCCCTGTCCTGATGTGTGGCTGGCCATGGCTCTCCAGCAGCAACAATAAAACCTGCTTACCCAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Klon 0039-T7-SequenzClone 0039 T7 sequence
AAAGCGCTGGAGCTACAGCCGTTACTGCCGCCGCCGCCGCCGCCGCCGCCGAGGCGTTTGATCGTTGGCAATGTCAGGCT TTGATAACTTAAACAGCGGTTTCTACCAGACGAGTTACAGCATCGACGAGCAATCTCAGCAGTCCTATGACTATGGAGGA AGTGGAGGACCCTACAGCAAGCAGTATGCTGGCTGWGACTACTCGCAGCAAGGCCGATTTGTCCCTCCAGACATGATGCA GCCACAGCAGACATACACTGGGCAGATTTACCAGCCAACTCAGGCCTATCCTCCAACAACACCTCAGCCATTCTATGGAG ACAGCTTTGAGGAGGAGCCCCCTCTGTTAGAΆGAGTTGGGTATCAATTTTGACCACATTTGGCAAAAAACACTAACGG G CTACACCCCCCTGAGGGCAGCTGACGGCAGCATCATGAATGAKNACGGACTTGGCAGGGCCAGTGGTGTTCTGCCTTGCC TTTGGGGCCACACTGTTACTGGCTGGCAAAATCCAGTTTGGCTACGTATACGGGATCAGTGCGATTGGATGTTTAGGAAT GNTTTGNTAAAGCGCTGGAGCTACAGCCGTTACTGCCGCCGCCGCCGCCGCCGCCGCCGAGGCGTTTGATCGTTGGCAATGTCAGGCT TTGATAACTTAAACAGCGGTTTCTACCAGACGAGTTACAGCATCGACGAGCAATCTCAGCAGTCCTATGACTATGGAGGA AGTGGAGGACCCTACAGCAAGCAGTATGCTGGCTGWGACTACTCGCAGCAAGGCCGATTTGTCCCTCCAGACATGATGCA GCCACAGCAGACATACACTGGGCAGATTTACCAGCCAACTCAGGCCTATCCTCCAACAACACCTCAGCCATTCTATGGAG ACAGCTTTGAGGAGGAGCCCCCTCTGTTAGAΆGAGTTGGGTATCAATTTTGACCACATTTGGCAAAAAACACTAACGG G CTACACCCCCCTGAGGGCAGCTGACGGCAGCATCATGAATGAKNACGGACTTGGCAGGGCCAGTGGTGTTCTGCCTTGCC TTTGGGGCCACACTGTTACTGGCTGGCAAAATCCAGTTTGGCTACGTATACGGGATCAGTGCGATTGGATGTTTAGGAAT GNTTTGNT
Klon 0040-T7-SequenzClone 0040-T7 sequence
CTCTCCTTTGCCCTGCGCACGGGTACACGGCACGGTGTGGACACTCACCTGTTCAGTGTGGAGTCGCCGCAAGAGCTGGCCTCTCCTTTGCCCTGCGCACGGGTACACGGCACGGTGTGGACACTCACCTGTTCAGTGTGGAGTCGCCGCAAGAGCTGGC
AGCCTGGACCCGACÄGTTGG GGATGGCTGTCATCGGSCTGCTGAAGGCGTACAAGAAGTGTCTACAGCCTGCACGTGGAAGCCTGGACCCGACÄGTTGG GGATGGCTGTCATCGGSCTGCTGAAGGCGTACAAGAAGTGTCTACAGCCTGCACGTGGA
ACGGCCGTCCCTGCAGCCTGTCTGTGCACATCGACAAGGGCTTCACCCTGTGGGCAGCTGAGCCTGGAGCAGCCCGAGCCACGGCCGTCCCTGCAGCCTGTCTGTGCACATCGACAAGGGCTTCACCCTGTGGGCAGCTGAGCCTGGAGCAGCCCGAGCC
ATGCTGCTCCGACAGCCCTTCGAGAAACTTCAGATGTCATCAGATGATGGCACGAGTCTCCTTTTCCTGGACTTTGGGGGATGCTGCTCCGACAGCCCTTCGAGAAACTTCAGATGTCATCAGATGATGGCACGAGTCTCCTTTTCCTGGACTTTGGGGG
TGCTGAAGGAGAGATCCAGCTGGACCTGCACTCGWGTCCCAAAACGATGGTCCTTTATCATCCACTCTTTCCTGTCCGCCTGCTGAAGGAGAGATCCAGCTGGACCTGCACTCGWGTCCCAAAACGATGGTCCTTTATCATCCACTCTTTCCTGTCCGCC
AAGGTCACCCGCTTGGGGCTCTTGGCCTAGAAGCTGTCAGATGCACCAGCCCTGAAGAGGATCGTTCATCTTGTGGCCTGAAGGTCACCCGCTTGGGGCTCTTGGCCTAGAAGCTGTCAGATGCACCAGCCCTGAAGAGGATCGTTCATCTTGTGGCCTG
ACCTGTCCTTCTGCTGACTGCCTGCTCGTCTGGGGGCTGAGGGAAGGGAGAGGAGGGGAACTAGGGCCTCANAGACCAGCACCTGTCCTTCTGCTGACTGCCTGCTCGTCTGGGGGCTGAGGGAAGGGAGAGGAGGGGAACTAGGGCCTCANAGACCAGC
CTGAGGAAGCAGAACTCAGACCTGGGACTGAGGAAGCAGAACTCAGACCTGGGA
Klon 0041-T7-SequenzClone 0041-T7 sequence
AGGCTGTGCGAAGCGGGCACGCGCGGACCCTKCACCGGCCGCGGAGCCGCTATGGGCCCGCCAGGCCCCTGGCGTGGCGCAGGCTGTGCGAAGCGGGCACGCGCGGACCCTKCACCGGCCGCGGAGCCGCTATGGGCCCGCCAGGCCCCTGGCGTGGCGC
GGCGCAAGGACCGGCTCCGAGCCAGGATTGAAATTCCAAGATGGATGATCAGGACCCTGGGGGCATTAGCCCCCTTCAGCGGCGCAAGGACCGGCTCCGAGCCAGGATTGAAATTCCAAGATGGATGATCAGGACCCTGGGGGCATTAGCCCCCTTCAGC
AAATGGTGGCCTCAGGAGCCGGGGCTGTGGWCACCTCCCTCTTCATGACACCCCTGGATGTGGTGAAGGTCCGCCTTCAGAAATGGTGGCCTCAGGAGCCGGGGCTGTGGWCACCTCCCTCTTCATGACACCCCTGGATGTGGTGAAGGTCCGCCTTCAG
TCTCAGAGACCCTCGGCAACCAGCGAATTGACAACTCCCTCCAGATTCTGGAGTCTCTCCTACACCAAATCATCCTCCGCTCTCAGAGACCCTCGGCAACCAGCGAATTGACAACTCCCTCCAGATTCTGGAGTCTCTCCTACACCAAATCATCCTCCGC
TCTACAGTCCCCAGGGAAGTGCCTCCTATACTGCAATGGAGTCCTGGAGCCCCTGTACCTGTGCCCAAATGGTACCCGCTTCTACAGTCCCCAGGGAAGTGCCTCCTATACTGCAATGGAGTCCTGGAGCCCCTGTACCTGTGCCCAAATGGTACCCGCT
GTGCCACCTGGTTTCAGGACCCCACACGGTTCACTGGCACCTTGGATGCCTTTG GAAGATTGTGCGGCATGAGGGCACTGTGCCACCTGGTTTCAGGACCCCACACGGTTCACTGGCACCTTGGATGCCTTTG GAAGATTGTGCGGCATGAGGGCACT
AGGACCCTGTGGAGCGGCCTCCCAGCCACCCTGGTGATGACCGYGCCAGCTACTGCTATCTACTTCACTGCTTACGACCAAGGACCCTGTGGAGCGGCCTCCCAGCCACCCTGGTGATGACCGYGCCAGCTACTGCTATCTACTTCACTGCTTACGACCA
ACTCAAGGCCTTCCTGTGTGGTCAGTCCTTGACCTCTGACCTCTACGCACCCATGGYGGCTGNTGCCCCTCGCCCCGAATACTCAAGGCCTTCCTGTGTGGTCAGTCCTTGACCTCTGACCTCTACGCACCCATGGYGGCTGNTGCCCCTCGCCCCGAAT
GGGCACCGTGACAGTTGCAGCCCCTTGNAGCTCGTGCGGACCAAGCTGCAGGCTCAGCATGTGTCATACCGTGAGCTGGCGGGCACCGTGACAGTTGCAGCCCCTTGNAGCTCGTGCGGACCAAGCTGCAGGCTCAGCATGTGTCATACCGTGAGCTGGC
TTNCTCTGTTAÄGCTGCNGTTNCTCTGTTAÄGCTGCNG
Klon 00 1-BGH-SequenzClone 00 1 BGH sequence
TTTTTTTTTTTTTTTTTTTTTTTTCACAGAAGACCATCATCTTTAGACAAGGAATGAATGGGGCCAAGATACTGCCTGGCTTTTTTTTTTTTTTTTTTTTTTTTCACAGAAGACCATCATCTTTAGACAAGGAATGAATGGGGCCAAGATACTGCCTGGC
CCAAGCTCCTCGGGCCAAGCTATAACAAACATGGGGATACAAAAAAGGGCGATCATCAGATTTGGTCTTGAATTCGGGAGCCAAGCTCCTCGGGCCAAGCTATAACAAACATGGGGATACAAAAAAGGGCGATCATCAGATTTGGTCTTGAATTCGGGAG
GCGGGAGAGGGGCAATCCTGGGAAGGGAAACAGCAGGAATCCAGGTCTGAGGACAGCCTGGG GACTGGACTGGGAGGGAGCGGGAGAGGGGCAATCCTGGGAAGGGAAACAGCAGGAATCCAGGTCTGAGGACAGCCTGGG GACTGGACTGGGAGGGA
AGGCACTTGGCTCAGTCTCCTGATCCCACCCGTGCAAGAGCCGGTTGCTCCCCGACTTTCAGCGGCCCANAGGCTGCTCC TGGTTGAGCCTCTGGAAGAAGCTTTTGCCAAACTCGTAAGTGCTGATCATGATGGCACΑGGAGGGGTGCAGCCTTGATGA TCCTGGGGAGGAAAGCCTGCAAAGAGTCCCCTGGTGCCAGATTCAGCCCGGAGGCACTTGGCTCAGTCTCCTGATCCCACCCGTGCAAGAGCCGGTTGCTCCCCGACTTTCAGCGGCCCANAGGCTGCTCC TGGTTGAGCCTCTGGAAGAAGCTTTTGCCAAACTCGTAAGTGCTGATCATGATGGCACΑGGAGGGGTGCAGCCTTGATGA TCCTGGGGAGGAAAGCCTGCAAAGAGTCCCCTGGTGCCAGATTCAGCCCGG
Klon 0042-T7-SequenzClone 0042-T7 sequence
AAAGCTTCACTTCGCCCTCCAGCCGCGG GGCTGCAGCGCAACTTCCAGATAGCGGAGTGGCCTCAGCTGCGAGCCGAGCAAAGCTTCACTTCGCCCTCCAGCCGCGG GGCTGCAGCGCAACTTCCAGATAGCGGAGTGGCCTCAGCTGCGAGCCGAGC
GGWGGCGGCAGCGYYCCTCAGGACACCCGCAGATCACCTTTTCCCCGCGACTTCGCCATGGCTGAGTGCTGWGTACCGGTGGWGGCGGCAGCGYYCCTCAGGACACCCGCAGATCACCTTTTCCCCGCGACTTCGCCATGGCTGAGTGCTGWGTACCGGT
ATGCCCACGGCCGATGTGTATCCCTCCACCCTATGCTGACCTCGGCAÄAGCTGCCAGAGACATTTTCAACAAAGGATTTGATGCCCACGGCCGATGTGTATCCCTCCACCCTATGCTGACCTCGGCAÄAGCTGCCAGAGACATTTTCAACAAAGGATTTG
GCTTWGGGCTGGTGAAGCTGGATGTGAAGACGAAGTCATGCAGCGGTGTGGAATTTTCAACATCTGGCTCATCTAATACAGCTTWGGGCTGGTGAAGCTGGATGTGAAGACGAAGTCATGCAGCGGTGTGGAATTTTCAACATCTGGCTCATCTAATACA
GACACTGGTAAAGTTAGCGGGACCTTGGAGACCAAGTACAAATGGTG GAGTATGGTCTGACTTTCACAGAGAAGTGGAAGACACTGGTAAAGTTAGCGGGACCTTGGAGACCAAGTACAAATGGTG GAGTATGGTCTGACTTTCACAGAGAAGTGGAA
CACCGATAACACTCTGGGGACAGAGATTGCAATTTGAAGACCAGATTTGTCAAGGWT GAAACTTGACTTTTGACACCCACACCGATAACACTCTGGGGACAGAGATTGCAATTTGAAGACCAGATTTGTCAAGGWT GAAACTTGACTTTTGACACCCA
CCTTTTCACCGAACACAGGAAAGAAAAGTGGTAAAATCAAGTCTGCTTACAAGAGGGAGTGTATAAACCTCGGCTGTGATCCTTTTCACCGAACACAGGAAAGAAAAGTGGTAAAATCAAGTCTGCTTACAAGAGGGAGTGTATAAACCTCGGCTGTGAT
GTTGACTTTGATTTTGCTGGACCTGCCATCCATGGGTCAGCTGTCTTTGGTTACGAGGGCTGGCTTGCTGGGTACCAAATGTTGACTTTGATTTTGCTGGACCTGCCATCCATGGGTCAGCTGTCTTTGGTTACGAGGGCTGGCTTGCTGGGTACCAAAT
GACCTTTGACAGTGCCAAGTCAAAGCTGACAAGGAGTGACCTTTGACAGTGCCAAGTCAAAGCTGACAAGGAGT
Klon 0042-BGH-SequenzClone 0042 BGH sequence
TTTTTTTTTTTTTTTTCGGTTTTCATAAACGTCTATTTCATCATTGGTGGGTAGCACATTTAACAGTTAAATACATTTAATTTTTTTTTTTTTTTTCGGTTTTCATAAACGTCTATTTCATCATTGGTGGGTAGCACATTTAACAGTTAAATACATTTAA
ATAATGTATAGGAGGCCGCACCACGGCAGCACTGATAACCATCCAACTAGGAACCAGCCAACAGTGACTGTCTAAATATTATAATGTATAGGAGGCCGCACCACGGCAGCACTGATAACCATCCAACTAGGAACCAGCCAACAGTGACTGTCTAAATATT
TAAAATACAGCTCTTGCTTCATCATCCTTTGATGTGATCACCTTCTGGGGGAAGGAAGGGGAGCCTGCTGGTCGCATGGATAAAATACAGCTCTTGCTTCATCATCCTTTGATGTGATCACCTTCTGGGGGAAGGAAGGGGAGCCTGCTGGTCGCATGGA
AATATATTAAGGCCAAATCTTCTGATATTCGTTCCCAGAGGTTTCTTTTAACTGGATTAAGCCTCCAATTCCAAGGCAAGAATATATTAAGGCCAAATCTTCTGATATTCGTTCCCAGAGGTTTCTTTTAACTGGATTAAGCCTCCAATTCCAAGGCAAG
CCCAAGTTTGTGGCCTCCAGCATTAAAGCTCTTCCCGTCTACCAGAGCAGACAGTGTAAGCTTCACACCAGGCCTCAGAGCCCAAGTTTGTGGCCTCCAGCATTAAAGCTCTTCCCGTCTACCAGAGCAGACAGTGTAAGCTTCACACCAGGCCTCAGAG
TCTGAGTATAGCCCACTCCAATTAAACTAGAGTTGTTGACCTTTGCAGAGATAGAAGCAGTAGGATCCAACTGGTATTTATCTGAGTATAGCCCACTCCAATTAAACTAGAGTTGTTGACCTTTGCAGAGATAGAAGCAGTAGGATCCAACTGGTATTTA
GCTGCAATGCCAAAACGAGTGCAGCTGGTACCCTGATGTCCAAGCGAGGNTTACTGAAGTGTCAAAATCTTCACATACTTGCTGCAATGCCAAAACGAGTGCAGCTGGTACCCTGATGTCCAAGCGAGGNTTACTGAAGTGTCAAAATCTTCACATACTT
TCTGATAAATTTGATCCTCCAAATTCTGTCCCATTATTTACATTTGTGTGNAGCTGGAAGTCCCCAGTCCTGTAGCCGACTCTGATAAATTTGATCCTCCAAATTCTGTCCCATTATTTACATTTGTGTGNAGCTGGAAGTCCCCAGTCCTGTAGCCGAC
NGCANGCA
Klon 0043-BGH-SequenzClone 0043 BGH sequence
TTTTTTTT TTTTTTTTTTTTAAGTT^ TTTTTTTT TTTTTTTTTTTTAAGTT ^
CGGGCATCTGTGCAΆACAGATTCATTTAACAGGTCGTAGTTTAAAAAAGTCATAGATACTGTGAGTTCTGTATAAACCGG TGGACGGCAAGTTAGTTCCTTTTGATTTATAAGCCTCAATGTCACCGCAGAATAAΆGAATGTAGCCAAAGAΆAGCATTAT CGGTCACTCGTATAGGACAGWGTTGTTTCTATAATTTGAAGCTTTCTGAATGGACGGGTTCAGGCCTGATCCAACTGTAA AAAGATCACTCAGTGAATAGACTATATGGGAACTGTACAAAGTGTCATTAACTTCCATCATTAATAGCTTACTCAGCACT ATACCACTATTGCTAGTTAAAATAACCTGCTTCTGAGGCCCCACGGAGGGAGGCGGCCTGTGCACGCAGCCTCGATGCCC TGGCCACCCTCATCCCCAGGGCGTGCCATACAGTCCAACAGAAAACTTTGGCTTTAGGAAGGAATCACAGACATTGAAAA GAATGGCTTTAATCATTATTAAATGTGCAGTGGGAAGGAGTGTGCTTCAGATAGTCTGGGCAGGGCTGGCGGCAGGCAGG TCACTCCTGCTGCACAGCTGCAGACACTAGTTTGTCATGACAAGACAATGAGGGAAAGCAGNCCGGGCATCTGTGCAΆACAGATTCATTTAACAGGTCGTAGTTTAAAAAAGTCATAGATACTGTGAGTTCTGTATAAACCGG TGGACGGCAAGTTAGTTCCTTTTGATTTATAAGCCTCAATGTCACCGCAGAATAAΆGAATGTAGCCAAAGAΆAGCATTAT CGGTCACTCGTATAGGACAGWGTTGTTTCTATAATTTGAAGCTTTCTGAATGGACGGGTTCAGGCCTGATCCAACTGTAA AAAGATCACTCAGTGAATAGACTATATGGGAACTGTACAAAGTGTCATTAACTTCCATCATTAATAGCTTACTCAGCACT ATACCACTATTGCTAGTTAAAATAACCTGCTTCTGAGGCCCCACGGAGGGAGGCGGCCTGTGCACGCAGCCTCGATGCCC TGGCCACCCTCATCCCCAGGGCGTGCCATACAGTCCAACAGAAAACTTTGGCTTTAGGAAGGAATCACAGACATTGAAAA GAATGGCTTTAATCATTATTAAATGTGCAGTGGGAAGGAGTGTGCTTCAGATAGTCTGGGCAGGGCTGGCGGCAGGCAGG TCACTCCTGCTGCACAGCTGCAGACACTAGTTTGTCATGACAAGACAATGAGGGAAAGCAGNC
Klon 0044-ContigClone 0044 contig
AAAGAAGAAGAGGGGGCTAAGCTGAGTATAGAGGTGCTCCAGACCAGCCTGCAGAAGGAACTGACTCTAAACAAAGGCCAAAAGAAGAAGAGGGGGCTAAGCTGAGTATAGAGGTGCTCCAGACCAGCCTGCAGAAGGAACTGACTCTAAACAAAGGCCA
GGCCTCCGCCATGGAGCTGCTGCGCTGCCCCACGCTTCGGCGCCTCTTCCTCTGCCTCTCTATGCTGTGGTTTGCCACTAGGCCTCCGCCATGGAGCTGCTGCGCTGCCCCACGCTTCGGCGCCTCTTCCTCTGCCTCTCTATGCTGTGGTTTGCCACTA
GCTTTGCCTACTACGGGCTGGTCATGGACCTGCAGGGCTTTGGGGTCAGCATGTACCTTATCCAGGTGATTTTCGGCGCTGCTTTGCCTACTACGGGCTGGTCATGGACCTGCAGGGCTTTGGGGTCAGCATGTACCTTATCCAGGTGATTTTCGGCGCT
GTGGACCTGCCTGCCAAGTTTGTGTGCTTCCTAGTCATCAATTCCATGGGCCGCCGGCCTGCACAGTTGGCCTCCCTGCTGTGGACCTGCCTGCCAAGTTTGTGTGCTTCCTAGTCATCAATTCCATGGGCCGCCGGCCTGCACAGTTGGCCTCCCTGCT
GCTGGCAGGCATCTGTATCCTAGTGAATGGCATAATACCGAGGGGCCATACAATCATTCGCACATCCCTGGCTGTACTAGGCTGGCAGGCATCTGTATCCTAGTGAATGGCATAATACCGAGGGGCCATACAATCATTCGCACATCCCTGGCTGTACTAG
GGAAAGGCTGTCTGGCTTCCTCTTTCAACTGCATCTTCCTGTACACCGGAGAGCTGTACCCCACAATGATTCGGCAGACGGGAAAGGCTGTCTGGCTTCCTCTTTCAACTGCATCTTCCTGTACACCGGAGAGCTGTACCCCACAATGATTCGGCAGACG
GGCCTGGGCATGGGCAGCACCATGGCCCGGGTGGGCAGCATAGTGAGCCCACTGATAAGCATGACTGCCGAGTTCTACCCGGCCTGGGCATGGGCAGCACCATGGCCCGGGTGGGCAGCATAGTGAGCCCACTGATAAGCATGACTGCCGAGTTCTACCC
CTCCATACCTCTCTTCATCTTCGGCGCTGTCCCCGTGGCCGCCAGCGCTGTCACTGCCCTGCTGCCAGAGACCTTGGGCCCTCCATACCTCTCTTCATCTTCGGCGCTGTCCCCGTGGCCGCCAGCGCTGTCACTGCCCTGCTGCCAGAGACCTTGGGCC
AGCCGCTGCCTGATACAGTGCAGGACCTGAAGAGCAGGAGCAGAGGAAAGCAGAAGCAACAGCAGCTGGAACAGCAGAAGAGCCGCTGCCTGATACAGTGCAGGACCTGAAGAGCAGGAGCAGAGGAAAGCAGAAGCAACAGCAGCTGGAACAGCAGAAG
CAGATGATACCACTCCAGGTCTCAACACAAGAGAAGAACGGACTCTGAAAATGGAGAGGGCGTCACTCAGCACTAAAGGGCAGATGATACCACTCCAGGTCTCAACACAAGAGAAGAACGGACTCTGAAAATGGAGAGGGCGTCACTCAGCACTAAAGGG
AGTGGGGTTCTGCAGGTCCCACCATCTATGAGGAGGGGGAGTGAGTGGAGGGACTGGGCCATCTAAATGCGGAGCCTGCCAGTGGGGTTCTGCAGGTCCCACCATCTATGAGGAGGGGGAGTGAGTGGAGGGACTGGGCCATCTAAATGCGGAGCCTGCC
ATTCAGAGAAATGCCTCCCCAAAGGTCACTTCAATAGACCTACTAGGAACAAAAGCTCTAACTGTGTGCAGCTTCTTAAGATTCAGAGAAATGCCTCCCCAAAGGTCACTTCAATAGACCTACTAGGAACAAAAGCTCTAACTGTGTGCAGCTTCTTAAG
CAGAATGAGCTCTTCCTCAGCCATCTTCCAGCTCAGTGGTCACTCCTCCAGCTCCTGTCCCTTCCACCTCCAGGACATTGCAGAATGAGCTCTTCCTCAGCCATCTTCCAGCTCAGTGGTCACTCCTCCAGCTCCTGTCCCTTCCACCTCCAGGACATTG
CAAAGAATCCCAGACAATTAAATAAATTTCTTCTACCTTGAAAAAAAAAAAAAAAAAACAAAGAATCCCAGACAATTAAATAAATTTCTTCTACCTTGAAAAAAAAAAAAAAAAAA
Klon 0045-ContigClone 0045 contig
AGCAGGAACTGAAGTCTTTATGGCCTTCCCACTGGAGACCAGCCGCCTGGAAGGCAGATGGGAGCCTCTTTTTCCTGCAAAGCAGGAACTGAAGTCTTTATGGCCTTCCCACTGGAGACCAGCCGCCTGGAAGGCAGATGGGAGCCTCTTTTTCCTGCAA
TACAGTGGATACAGTTTGCTGTGGCTACTCTGAGTGTTATAGGCTCCAGTTCACTCCTCACCTACATCGCGTTCCAGAGCTACAGTGGATACAGTTTGCTGTGGCTACTCTGAGTGTTATAGGCTCCAGTTCACTCCTCACCTACATCGCGTTCCAGAGC
CCACAGAAGTCTGCAGAGATAAAGCCACTCTTGTATCTGAGCTTCTCTGACCTGCTCCTGGGAATTTGCTGGCTCGTAAACCACAGAAGTCTGCAGAGATAAAGCCACTCTTGTATCTGAGCTTCTCTGACCTGCTCCTGGGAATTTGCTGGCTCGTAAA
GGCACTTCTCTATGGAACTGCAGCAGCCCACAAGGACAGCATCTGCTATAACCTACAGACCGTGGGGGAGATTTTCTACCGGCACTTCTCTATGGAACTGCAGCAGCCCACAAGGACAGCATCTGCTATAACCTACAGACCGTGGGGGAGATTTTCTACC
TTGCCTCACTCCTCTACACCGTCAGCTACATCTGGTGTCTGTACTCGGAGCTGAGGAGGAAGAGCGGCCAGAGTGAGAGG AGCACGGTTGCCCAGGTGACAGATTATGTTTGCCAAGGTGGTCACATATTGTTCCTTTTGTCAAGCCTGATACCTCCGCT GCTGATGACGCCTGTGTTCCTTTTGGGCAATGTCAATGAATGTTTCCACAACTTCAGTGAGAGCCACAGGTGTATCCTAA TGCACTCACCGCCATCAGCCACGCCTGAGCCCCTGCCTTCCACCAATACATCTGTCTGCAGCACACTCTATTTTTATGGT GTCACCATTTTCCTGGCCAGCTTTTTCTTCAGCTTCCTCACCATCATGGTCTTACTCGTCCATGCCCAGATGTTATATAA GAAGTTTTGTRAÄGTCGACGGGCCTTCTGGAGAGTGAGCAGTGGGCAGTGATTCACACTGTGGGCCAGCGAGTGCGTTTC TTNNCAGTGGCCTTTGTGTGCTGCTGGGGTNCAGCTGTCTCCCTGCTGATCATGAAGCTGACAGAGCCTCAGGAGACCTT CCTGCACATGGCCCTCTCTGTGCTCCAGGCTCTCTCAGCAGCATCCCAGGGGCTGCTCAACTGTGGCWTTTATGGCTGGA CNCAGTGCAAGTTCCAGCAGATAAAGCGTGAGCCTCACCGTGACGCAGACACGCAGACCCCWCTGCTGTGCTCTCAGAAG AGAATTTATAGTAGAAGCCCAAACCTACTGGAGTCTCCTCTTGCCCTTGCTTCCAGCTCCTCCACCGTTCTTTGAGACCC TAGAATGGGAATGTCAGGGAGTGAATGTTTCACATGAGTGGACTCTCCTCTGGCCAAACCCGAGACTGGAGAGCTGAAAG GAAATGTGGGCTCAGGGTCAGTAGCAATTCTGGGTGAATTCAGAAGCAGCTCTCTAACTGCTCCTTCAGAGATATACAGC CGCTTTCCAT TAAGAGACTCGTTACTTTGACAAGAAAAaATAAATCTCAGGTTCATAAAAAAAAAAAAAAAAAAATTGCCTCACTCCTCTACACCGTCAGCTACATCTGGTGTCTGTACTCGGAGCTGAGGAGGAAGAGCGGCCAGAGTGAGAGG AGCACGGTTGCCCAGGTGACAGATTATGTTTGCCAAGGTGGTCACATATTGTTCCTTTTGTCAAGCCTGATACCTCCGCT GCTGATGACGCCTGTGTTCCTTTTGGGCAATGTCAATGAATGTTTCCACAACTTCAGTGAGAGCCACAGGTGTATCCTAA TGCACTCACCGCCATCAGCCACGCCTGAGCCCCTGCCTTCCACCAATACATCTGTCTGCAGCACACTCTATTTTTATGGT GTCACCATTTTCCTGGCCAGCTTTTTCTTCAGCTTCCTCACCATCATGGTCTTACTCGTCCATGCCCAGATGTTATATAA GAAGTTTTGTRAÄGTCGACGGGCCTTCTGGAGAGTGAGCAGTGGGCAGTGATTCACACTGTGGGCCAGCGAGTGCGTTTC TTNNCAGTGGCCTTTGTGTGCTGCTGGGGTNCAGCTGTCTCCCTGCTGATCATGAAGCTGACAGAGCCTCAGGAGACCTT CCTGCACATGGCCCTCTCTGTGCTCCAGGCTCTCTCAGCAGCATCCCAGGGGCTGCTCAACTGTGGCWTTTATGGCTGGA CNCAGTGCAAGTTCCAGCAGATAAAGCGTGAGCCTCACCGTGACGCAGACACGCAGACCCCWCTGCTGTGCTCTCAGAAG AGAATTTATAGTAGAAGCCCAAACCTACTGGAGTCTCCTCTTGCCCTTGCTTCCAGCTCCTCCACCGTTCTTTGAGACCC TAGAATGGGAATGTCAGGGAGTGAATGTTTCACATGAGTGGACTCTCCTCTGGCCAAACCCGAGACTGGAGAGCTGAAAG GAAATGTGGGCTCAGGGTCAGTAGCAATTCTGGGTGAATTCAGAAGCAGCTCTCTAACTGCTCCTTCAGAGATATACAGC CGCTTTCCAT TAAGAGACTCGTTACTTTGACAAGAAAAaATAAATCTCAGGTTCATAAAAAAAAAAAAAAAAAAA
Klon 0046-ContigClone 0046 contig
AAAAGTGGGCCCCGAGGTCGCGTCTTGCCCAAGTCTCCGCGGTCCCCAGCGCTCGCTCGCGCGGTCCTGCCACGGCCTTCAAAAGTGGGCCCCGAGGTCGCGTCTTGCCCAAGTCTCCGCGGTCCCCAGCGCTCGCTCGCGCGGTCCTGCCACGGCCTTC
CTGCTGCCCGCGCCATGGGCCTCAGCTCCAACTCCGCTGTGCRAGTTGAGTGGATCGCGGCCGTCACCTTTGCTGCTGGCCTGCTGCCCGCGCCATGGGCCTCAGCTCCAACTCCGCTGTGCRAGTTGAGTGGATCGCGGCCGTCACCTTTGCTGCTGGC
ACAGCCGCTCTCGGTTACCTGGCTTACAAGAAGTTCTACGCTAAAGAGAATCGC CCAAAGCTATGGTGAATCTTCAGATACAGCCGCTCTCGGTTACCTGGCTTACAAGAAGTTCTACGCTAAAGAGAATCGC CCAAAGCTATGGTGAATCTTCAGAT
CCAGAAAGWCAACCCGAAGGTGGTGCATGCCTTCGACATGGAGGATCTGGGGGATAAGGCCGTGTACTGCCGATGCTGGACCAGAAAGWCAACCCGAAGGTGGTGCATGCCTTCGACATGGAGGATCTGGGGGATAAGGCCGTGTACTGCCGATGCTGGA
GGTCTAAAAAGTTCCCCTTCTGCGATGGGGCTCACATAAAGCACAACGAAGAGACTGGCGACAACGTAGGACCTCTGATCGGTCTAAAAAGTTCCCCTTCTGCGATGGGGCTCACATAAAGCACAACGAAGAGACTGGCGACAACGTAGGACCTCTGATC
ATCAAGAAAAAGGAAACCTAATGGACAGTTGCGAGGCTGCACCCAGCGTGTTGTGATGTCACCTGCTGATTTACGTAGAAATCAAGAAAAAGGAAACCTAATGGACAGTTGCGAGGCTGCACCCAGCGTGTTGTGATGTCACCTGCTGATTTACGTAGAA
TGGCACCCAACCCACMBGTCTGATTGGCCTCCCCGGTTCTAGATGTGGTTGGTCCCTGCAAATCACAGCTCTCATATCCATGGCACCCAACCCACMBGTCTGATTGGCCTCCCCGGTTCTAGATGTGGTTGGTCCCTGCAAATCACAGCTCTCATATCCA
TGGCATCGGCCTTGCTACTGAAACATGTGGTGCACGTTTGTTGAAAGAAGAAGAAAGGCTAAACCAACCTCGTGCTATATTGGCATCGGCCTTGCTACTGAAACATGTGGTGCACGTTTGTTGAAAGAAGAAGAAAGGCTAAACCAACCTCGTGCTATAT
GGGTTATTTTGGTCTTGTAAGGATCCGTTCCTTTAAAATAATGGTCTTAGAATATAGTTGTATCTTGAGGTTAAAGTATTGGGTTATTTTGGTCTTGTAAGGATCCGTTCCTTTAAAATAATGGTCTTAGAATATAGTTGTATCTTGAGGTTAAAGTATT
AAATTATTCCAAAATCATG ACA G AAAAAAAAAAAAAAAAAAAAATTATTCCAAAATCATG ACA G AAAAAAAAAAAAAAAAAA
Klon 0047-ContigClone 0047 contig
AAAGGAGCAACGCGGGTCTTCCCGCTGTTGCTTGTCGCGGCCACGGCCGAGCATACGTCCCCGGCCTGAGGTGGTGGTGGAAAGGAGCAACGCGGGTCTTCCCGCTGTTGCTTGTCGCGGCCACGGCCGAGCATACGTCCCCGGCCTGAGGTGGTGGTGG
CGAGCCCACCGCCGTTTGCTGCGACCTCATGGAAGGTGGCGGAGGAAGTAGCAACAAATCCACCAGCGGGTTAGCTGGCTCGAGCCCACCGCCGTTTGCTGCGACCTCATGGAAGGTGGCGGAGGAAGTAGCAACAAATCCACCAGCGGGTTAGCTGGCT
TCTTCGGAGCCGGAGGAGCGGGTTACTCGAACGCTGATTTGGCCGGCGTCCCGCTGACTGGTATGAACCCCCTGTCTCCTTCTTCGGAGCCGGAGGAGCGGGTTACTCGAACGCTGATTTGGCCGGCGTCCCGCTGACTGGTATGAACCCCCTGTCTCCT
TATTTAAATGTGGATCCACGCTATCTCGTTCAGGATACTGATGAATTTATTTTGCCAACTGGAGCTAATAAAACCCGAGGTATTTAAATGTGGATCCACGCTATCTCGTTCAGGATACTGATGAATTTATTTTGCCAACTGGAGCTAATAAAACCCGAGG
CAGATTTGAACTAGCTTTCTTTACCATTGGAGGATGTTGCATGACAGGGGCCGCATTCGGGGCAATGAACGGTCTTCGTTCAGATTTGAACTAGCTTTCTTTACCATTGGAGGATGTTGCATGACAGGGGCCGCATTCGGGGCAATGAACGGTCTTCGTT
TAGGATTGAAGGAAACCCAGAGCATGGCCTGGTCCAAACCAAGAAATGTACAGATTTTGAATATGGTGACTAGGCAAGGATAGGATTGAAGGAAACCCAGAGCATGGCCTGGTCCAAACCAAGAAATGTACAGATTTTGAATATGGTGACTAGGCAAGGA
GCACTTTGGGCTAATACTCTAGGCTCCCTGGCTTTGCTCTATAGTGCTTTTGGTGTTATCATTGAGAAAACACGGGGTGCGCACTTTGGGCTAATACTCTAGGCTCCCTGGCTTTGCTCTATAGTGCTTTTGGTGTTATCATTGAGAAAACACGGGGTGC
AGAAGATGACCTCAACACAGTAGCAGCTGGAACCATGACCGGGATGYTGTATAAATGTACAGGTGGTCTTCGAGGAATAGAGAAGATGACCTCAACACAGTAGCAGCTGGAACCATGACCGGGATGYTGTATAAATGTACAGGTGGTCTTCGAGGAATAG
CACGCGGTGGCCTGGCAGGACTGACACTCACCAGTCTCTATGCACTGTATAACAÄCTGGGAGCACATGAAAGGTTCCCTGCACGCGGTGGCCTGGCAGGACTGACACTCACCAGTCTCTATGCACTGTATAACAÄCTGGGAGCACATGAAAGGTTCCCTG
CTCCAACAGTCACTCTGAAGATTTTTGGCACCTCAGGAATGGGGGACACTTCATAGTCATCCAGATCAATTAATAAGCCA GTCTGGAGTTGCTTTCTGTCTTGTACCTACAGTTACTTTGAACAATTGGAGACTCTGATTTGCTGTGACAAAGATCATGG ATGGTTAGCCTGGACTGCACTGCTTGCCCTCAGTGAGTGGAAGCCAACCCCTTGGTGGCTCACTGAGTATGTGTTCTCTT CTCCTTTGGAGATGATCTTGCACCCCAAACTGGAAAACCACTTACTCTCCAAATTCAGGCCATACTTTTTATTACCATGT TAATTCATTTAAAAAATCTAAAΆCTTAAATGTTGCCCTGTTTTCCAAATAAAGGGTGAAAACAGAAAAAAAAAAAAAAAA AAΆAAAACTCCAACAGTCACTCTGAAGATTTTTGGCACCTCAGGAATGGGGGACACTTCATAGTCATCCAGATCAATTAATAAGCCA GTCTGGAGTTGCTTTCTGTCTTGTACCTACAGTTACTTTGAACAATTGGAGACTCTGATTTGCTGTGACAAAGATCATGG ATGGTTAGCCTGGACTGCACTGCTTGCCCTCAGTGAGTGGAAGCCAACCCCTTGGTGGCTCACTGAGTATGTGTTCTCTT CTCCTTTGGAGATGATCTTGCACCCCAAACTGGAAAACCACTTACTCTCCAAATTCAGGCCATACTTTTTATTACCATGT TAATTCATTTAAAAAATCTAAAΆCTTAAATGTTGCCCTGTTTTCCAAATAAAGGGTGAAAACAGAAAAAAAAAAAAAAAA AAΆAAAA
Klon 0048-ContigClone 0048 contig
AGGGCCTCCGCACTTCCAAGTCATCTGCCGGCTACTTGGCTACCAGGGCATTGCAGTAGTCATGGAAGAGCTGCTGAAGG TTGTCAAGAGCCTGCTGCAAGGCACAATCCTACAGTACGTGAAAACTCTGATGGAAGTGATGCCCAAGATCTGCCGGCTT CCGCGACATGAGTACGGCTC CCTGGCATCCTGGAG TCTTCCACCACCAGCTGAAGGACATTGWGGAGATGCASNAGC TGAAAACCGTATGCTTCCAGAACCTGCGGSAGGTGGGMAATGCTGYYCTCTTCTGCCTGCTTAT GAGCAAAGCCTGTCT TTAGAAGAAGTCTGTGACCTGTTGCATGCAGCTCCTTTCCAGAATATCTTACCTCGAATCCATGTAAAAGAGGGGGAGAG AGTTGATGCCAÄAATGAAΆAGACTAGAATCCAAGTATGCCCCACTGCACCTTGTCCCACTGATTGAAAGGCTGGGGACCC CACAGCAAATTGCAATTGCAAGAGAGGGGGACTTGCTGACCAAGGAGCGTCTCTGTTGTGGTCTGTCCATGTTTGAAGTC ATCCTGACACGGATCCGGACCTTTCTGGATGATCCCATCTGGCGTGGGCCCCTACCCAGCAATGGTGTCATGCACGTGGA TGAGTGTGTGGAGTTTCACAGÄCTATGGAGTGCCATGCAGTTTGTTTACTGCATTCCTGTAGGGACACACGAGTTTACAG TGGGAGCAGTGTTTTGGAGATGGGCNTCCWCTGGGCTGGCTGCATGATCATTGTACTTCTTGGACAGCAGCGGCGCTTTG CTGTGTTGGATTTCTGCTATCATCNTCTCAAAGTTCAGAANCATGATGGCΆAAGATGAGATCATCAΆAAATGTGCCATTG AAGAAGATGGTGGAGAGGATCCGCAAGTTCCAGATTCTCAACGATGAAATCATCACTATCCTGGACAAGTACTTGAAATC TGGΓGATGGCGAGAGCNCNCCTGTGGAGCATGTACGCTGCTTCCAGCCNCCCNTCCTCCAGTCCCTAGCCNGTAGCTGAG GGCTAAGCTCNCAGCATCCCAGTAACTGATGGCATGTTTGTCTTTATGTAAACTATATTGAAATTTTTAGGGGCTATTTT CCNTTATGTCTGAACCTACTTTTGATCTGAAAGCTTAACTTTATAAAATTTACTTATTTTTCTATACTAAAATTGTATAT GCCTTTGGAATTGGGAAGTCTGAGAATACGGTTACTAGTTGTTGCCNTTACGGTATTAACAAGTATTTTCATGCCGAATA TTACAGCAGNTA Klon 0051-T7-SequenzAGGGCCTCCGCACTTCCAAGTCATCTGCCGGCTACTTGGCTACCAGGGCATTGCAGTAGTCATGGAAGAGCTGCTGAAGG TTGTCAAGAGCCTGCTGCAAGGCACAATCCTACAGTACGTGAAAACTCTGATGGAAGTGATGCCCAAGATCTGCCGGCTT CCGCGACATGAGTACGGCTC CCTGGCATCCTGGAG TCTTCCACCACCAGCTGAAGGACATTGWGGAGATGCASNAGC TGAAAACCGTATGCTTCCAGAACCTGCGGSAGGTGGGMAATGCTGYYCTCTTCTGCCTGCTTAT GAGCAAAGCCTGTCT TTAGAAGAAGTCTGTGACCTGTTGCATGCAGCTCCTTTCCAGAATATCTTACCTCGAATCCATGTAAAAGAGGGGGAGAG AGTTGATGCCAÄAATGAAΆAGACTAGAATCCAAGTATGCCCCACTGCACCTTGTCCCACTGATTGAAAGGCTGGGGACCC CACAGCAAATTGCAATTGCAAGAGAGGGGGACTTGCTGACCAAGGAGCGTCTCTGTTGTGGTCTGTCCATGTTTGAAGTC ATCCTGACACGGATCCGGACCTTTCTGGATGATCCCATCTGGCGTGGGCCCCTACCCAGCAATGGTGTCATGCACGTGGA TGAGTGTGTGGAGTTTCACAGÄCTATGGAGTGCCATGCAGTTTGTTTACTGCATTCCTGTAGGGACACACGAGTTTACAG TGGGAGCAGTGTTTTGGAGATGGGCNTCCWCTGGGCTGGCTGCATGATCATTGTACTTCTTGGACAGCAGCGGCGCTTTG CTGTGTTGGATTTCTGCTATCATCNTCTCAAAGTTCAGAANCATGATGGCΆAAGATGAGATCATCAΆAAATGTGCCATTG AAGAAGATGGTGGAGAGGATCCGCAAGTTCCAGATTCTCAACGATGAAATCATCACTATCCTGGACAAGTACTTGAAATC TGGΓGATGGCGAGAGCNCNCCTG TGGAGCATGTACGCTGCTTCCAGCCNCCCNTCCTCCAGTCCCTAGCCNGTAGCTGAG GGCTAAGCTCNCAGCATCCCAGTAACTGATGGCATGTTTGTCTTTATGTAAACTATATTGAAATTTTTAGGGGCTATTTT CCNTTATGTCTGAACCTACTTTTGATCTGAAAGCTTAACTTTATAAAATTTACTTATTTTTCTATACTAAAATTGTATAT GCCTTTGGAATTGGGAAGTCTGAGAATACGGTTACTAGTTGTTGCCNTTACGGTATTAACAAGTATTTTCATGCCGAATA TTACAGCAGNTA Clone 0051-T7 sequence
AAAGCCTGTGTACGCCACCATCGGCTYYNGGTATCGTCAACACGGCCTTCACTGTGGWGTCGCTGTTTGTTGTAGAGCGAAAAGCCTGTGTACGCCACCATCGGCTYYNGGTATCGTCAACACGGCCTTCACTGTGGWGTCGCTGTTTGTTGTAGAGCGA
GCTGGACGACGGACCCTGCACCTCATTGGCCTGGCTGGCATGGCAGGCTGWGCTGTGCTCATGACCATCGCCCTGGCCTTGCTGGACGACGGACCCTGCACCTCATTGGCCTGGCTGGCATGGCAGGCTGWGCTGTGCTCATGACCATCGCCCTGGCCTT
GCTGGAACGGCTGCCTTGGATGTCCTATCTGAGCATCGTGGCCATCTTTGGCT GTGGCCTTCTTTGAAGTAGGCCCTGGCTGGAACGGCTGCCTTGGATGTCCTATCTGAGCATCGTGGCCATCTTTGGCT GTGGCCTTCTTTGAAGTAGGCCCTG
GTCCTATTCCATGGYTCATTGTGGCCGAGCTGTTCAGCCAGGGGCCCCGTCCTGCTGCTATTGCTGAGGCTGGCTTCTCCGTCCTATTCCATGGYTCATTGTGGCCGAGCTGTTCAGCCAGGGGCCCCGTCCTGCTGCTATTGCTGAGGCTGGCTTCTCC
AACTGGACCTCAAACTTCATTGTGGGCATGTGCTTCCAGTATGTGGAGCAACTGTGCGGCCCCTACGTCCTTCATCATCTAACTGGACCTCAAACTTCATTGTGGGCATGTGCTTCCAGTATGTGGAGCAACTGTGCGGCCCCTACGTCCTTCATCATCT
TCACGG GCTCCTCGTGCTCTTCTTCATCTTCACCTACTTCAAAGTCCCTGAGACCAAAGGCCTGAACCTTCGATGAGATTCACGG GCTCCTCGTGCTCTTCTTCATCTTCACCTACTTCAAAGTCCCTGAGACCAAAGGCCTGAACCTTCGATGAGAT
CGCTTCCGGCTTCCGGCAGGGGGGTGCCAGCCAAAGTGACAAGACACCCGAGGAGCTCTTCCACCCTCTGGGGGCGGACTCGCTTCCGGCTTCCGGCAGGGGGGTGCCAGCCAAAGTGACAAGACACCCGAGGAGCTCTTCCACCCTCTGGGGGCGGACT
CCCAAGTGTGAGGAGCCCCACACCCAGCCCCGGCCTGCTCCCTGCAGCCCAAGGATCTCTCTGGAGCACAGGCAGCTAGACCCAAGTGTGAGGAGCCCCACACCCAGCCCCGGCCTGCTCCCTGCAGCCCAAGGATCTCTCTGGAGCACAGGCAGCTAGA
TGAGACCTCTTNCGAACCGACAGATCTCGGGCAAGCCGGGCCTGGGCGCCTTTCCTCAGTGAGACCTCTTNCGAACCGACAGATCTCGGGCAAGCCGGGCCTGGGCGCCTTTCCTCAG
Klon 0052-T7-SequenzClone 0052-T7 sequence
AAAGCTTACCCAGCAGSRNGAGTAGATTTGTATCAACAAGAGAAGGCACGGATGGGCTCAGCGGGACTCAGAGTTCTTGCAAAGCTTACCCAGCAGSRNGAGTAGATTTGTATCAACAAGAGAAGGCACGGATGGGCTCAGCGGGACTCAGAGTTCTTGC
CTTGGCGTCTGGTCCGGAACTGGGGCAGCTGACCTTCCTTGGCCTGG CGGAATCATTGACCCTCCTCGAACTGGTGTGACTTGGCGTCTGGTCCGGAACTGGGGCAGCTGACCTTCCTTGGCCTGG CGGAATCATTGACCCTCCTCGAACTGGTGTGA
AGGAAGCTGTCACAACACTCATTGCCTCAGGAGTCTCCATYAAAATGATCACTGGAGATTCTCAGGAGACTGCAATTGCCAGGAAGCTGTCACAACACTCATTGCCTCAGGAGTCTCCATYAAAATGATCACTGGAGATTCTCAGGAGACTGCAATTGCC
ATCGCTAGWCGTCTGGGATTGTACTCTAAGACTTCACAGTCCG GTCTGGGGAAGAAGTCGATACAATGGAGGTGCAGCAATCGCTAGWCGTCTGGGATTGTACTCTAAGACTTCACAGTCCG GTCTGGGGAAGAAGTCGATACAATGGAGGTGCAGCA
CCTTTCACAGATAG GCCAAAGGTTGYAGTATTTTACAGAGCAAGCCCAAGACACAAGATGAAAATTATTAAGTCTCTACCCTTTCACAGATAG GCCAAAGGTTGYAGTATTTTACAGAGCAAGCCCAAGACACAAGATGAAAATTATTAAGTCTCTAC
AGAAGAACGGGGCAGTTGTAGCCATGACAGGAGATGGGGTAAATGATGCAGTTGCTCTCAAGGCTGCAGACATTGGAGTTAGAAGAACGGGGCAGTTGTAGCCATGACAGGAGATGGGGTAAATGATGCAGTTGCTCTCAAGGCTGCAGACATTGGAGTT
GCGATGGGCCAGACTGGCACCGATGTTTGCAAAGAGGCTGCAGACATGATCTTGGTGAATGATGATTTCCAAACCATCATGCGATGGGCCAGACTGGCACCGATGTTTGCAAAGAGGCTGCAGACATGATCTTGGTGAATGATGATTTCCAAACCATCAT
GTCTGCAATAGAAGAGGGTAAAGGCATTTATAATAACATTAAAAATTTTGCTAGATTTCAACTGAGCACGAGTATAGCAGGTCTGCAATAGAAGAGGGTAAAGGCATTTATAATAACATTAAAAATTTTGCTAGATTTCAACTGAGCACGAGTATAGCAG
CATTAACTTTAATCTCATTGGCTACGTTAATGAACTTTCCTAACCCTCTCAATGCAATGCAGATTTTGTGGATCAATATTCATTAACTTTAATCTCATTGGCTACGTTAATGAACTTTCCTAACCCTCTCAATGCAATGCAGATTTTGTGGATCAATATT
ATAATGGATGGACCTCCAGCTCAGAGCCTTGGAGTAGAGCCAGNGGATAAAGATGTCATTCGAAAACCGNCTCGAAAATAATGGATGGACCTCCAGCTCAGAGCCTTGGAGTAGAGCCAGNGGATAAAGATGTCATTCGAAAACCGNCTCGAAA
Klon 0052-BGH-SequenzClone 0052 BGH sequence
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCCYTTAAATGATTGAGTTTATTTGTGKGTTAGTAAGAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCCYTTAAATGATTGAGTTTATTTGTGKGTTAGTAAGAAA
GCCCYAYAACCATAAATAGTACAATATTTAAAAGTAAAAAAAATATTTATATCCATCTAAGACAGACAGTGTATTTGTACGCCCYAYAACCATAAATAGTACAATATTTAAAAGTAAAAAAAATATTTATATCCATCTAAGACAGACAGTGTATTTGTAC
ATTAGAATTCTTTAAGTGCAGAAGGTGGTTCAGGTTTTGCCTTTTGKATTTTTTAATTCAGTACTTGCCCATATGTTGAAATTAGAATTCTTTAAGTGCAGAAGGTGGTTCAGGTTTTGCCTTTTGKATTTTTTAATTCAGTACTTGCCCATATGTTGAA
GTTCACTCAATTGCAAAAGCCAATTCAAATCAAGGAATGTGTTATTCAAAGKTGCATAATTTCATTTGGGACTACCAACAGTTCACTCAATTGCAAAAGCCAATTCAAATCAAGGAATGTGTTATTCAAAGKTGCATAATTTCATTTGGGACTACCAACA
AAGTTCAAATCAAGTCTTTAACATTAATGGGTCATTTTTAGTCAATGGAAAGTTAAAAAGTCCTTAAGTCTTCATTATCTAAGTTCAAATCAAGTCTTTAACATTAATGGGTCATTTTTAGTCAATGGAAAGTTAAAAAGTCCTTAAGTCTTCATTATCT
TCTTGAACTTGCCCTTCTAAATGATTCTNAGACTGCANTNCCTAGTTTGCNAATTAAAAAAAAATGCNNTATGCATCATATCTTGAACTTGCCCTTCTAAATGATTCTNAGACTGCANTNCCTAGTTTGCNAATTAAAAAAAAATGCNNTATGCATCATA
CTTNAAGGAAAGACGATGAANGCTNAACCAGCNTTCTTCCTGGACCTTCTCCCGGCTCCTTTCANCCTTCTTTATAACCCCTTNAAGGAAAGACGATGAANGCTNAACCAGCNTTCTTCCTGGACCTTCTCCCGGCTCCTTTCANCCTTCTTTATAACCC
TCAGNCACATCAGNCACA
Klon 0055-ContigClone 0055 contig
AGGATTGGCGGGGTTCTCTTTGACCACATAAATCAATTCTTTCTTTTGGGAGCCTCAATGGTGGCAA.CTGCAGCGGGACTAGGATTGGCGGGGTTCTCTTTGACCACATAAATCAATTCTTTCTTTTGGGAGCCTCAATGGTGGCAA.CTGCAGCGGGACT
TTATCTCATCCCGTTCTGCAAGACAGCAGTCTTATTGATCATCACCATGTCTGTCTTTGGAGCTTCCGTTGGTGTTGTGGTTATCTCATCCCGTTCTGCAAGACAGCAGTCTTATTGATCATCACCATGTCTGTCTTTGGAGCTTCCGTTGGTGTTGTGG
ATACAGGTGCAAATGTTCTCATCTTGGATCTCTGGGGGGACAAAGGAGCCCCACAAATGCAGGCCTTGCACTTCAGTTTCATACAGGTGCAAATGTTCTCATCTTGGATCTCTGGGGGGACAAAGGAGCCCCACAAATGCAGGCCTTGCACTTCAGTTTC
GCεTTGGGTGCCTTTCTGGCTCCCCTGCTGGCTAAGTTGGCCTGGGGTACAGCACCTGCTCAGAACCACACCGAGTCCGAGCεTTGGGTGCCTTTCTGGCTCCCCTGCTGGCTAAGTTGGCCTGGGGTACAGCACCTGCTCAGAACCACACCGAGTCCGA
CCTTGACACTCTGATGCTGAACCGATCCTCCAACGGCACCTCAGACTCCGTGTTTGCGGTACCCGATGACATGAATCTGCCCTTGACACTCTGATGCTGAACCGATCCTCCAACGGCACCTCAGACTCCGTGTTTGCGGTACCCGATGACATGAATCTGC
TGTGGGCATATGCTTCCATTGGCACCTTTATTTTAGTAGTTTCTGTCTTTCTGTTTGGTCTGTTTTGTAAGAAACATTCATGTGGGCATATGCTTCCATTGGCACCTTTATTTTAGTAGTTTCTGTCTTTCTGTTTGGTCTGTTTTGTAAGAAACATTCA
AGGCAGAAAAAACCCAGAGCATCTGCTGAGGGAGCTCGAAGGGCTAAATATCACAGGGCCCTGCTATGCCTGCTCTTCCTAGGCAGAAAAAACCCAGAGCATCTGCTGAGGGAGCTCGAAGGGCTAAATATCACAGGGCCCTGCTATGCCTGCTCTTCCT
CTTCTTCTTCTTCTATGTGGGAGCCGAGATAACATACGGCTCTTACATATTCTCCTTCGCCACCACCCATGTTGGCATGGCTTCTTCTTCTTCTATGTGGGAGCCGAGATAACATACGGCTCTTACATATTCTCCTTCGCCACCACCCATGTTGGCATGG
AAGAGAGCGAGGCAGCTGGCTTGAATTCCATCTTCTGGGGAACCTTTGCAGCCTGCAGGGGCCTGGCCATCTTCTTTGCGAAGAGAGCGAGGCAGCTGGCTTGAATTCCATCTTCTGGGGAACCTTTGCAGCCTGCAGGGGCCTGGCCATCTTCTTTGCG
ACATTCTTACAGCCTGGAACCATGATCGTGCTGAGCAACATTGGCAGCCTGGTCTCÄTGTTTCTTTCTGGTACTTTTTGAACATTCTTACAGCCTGGAACCATGATCGTGCTGAGCAACATTGGCAGCCTGGTCTCÄTGTTTCTTTCTGGTACTTTTTGA
CAAGAGCCCTCTTTGTCTCTGGATCGCGACTTCTGTGTATGGAGCCTCAATGGCAGCCACGTTTCCCAGCGGCATCTCCTCAAGAGCCCTCTTTGTCTCTGGATCGCGACTTCTGTGTATGGAGCCTCAATGGCAGCCACGTTTCCCAGCGGCATCTCCT
GGATTGAGCAGTACACCACCTTAACTGGGAAATCTGCAGCATTCTTTGTAATTGGCTCTGCCCTGGGAGATATGGCCATTGGATTGAGCAGTACACCACCTTAACTGGGAAATCTGCAGCATTCTTTGTAATTGGCTCTGCCCTGGGAGATATGGCCATT
CCAGCGGTGATCGGAATTCTTCAGGGACACTACCCAGATCTGCCAGTAGTTCTGTACACATGTCTGGGCTCAGCCATATTCCAGCGGTGATCGGAATTCTTCAGGGACACTACCCAGATCTGCCAGTAGTTCTGTACACATGTCTGGGCTCAGCCATATT
CACAGCTATTTTATTTCCTGTGATGTATAAATTAGCTACCTTGCCCCTGAAGAGAGAGGACCAGAAAGCTTTGCCCACTACACAGCTATTTTATTTCCTGTGATGTATAAATTAGCTACCTTGCCCCTGAAGAGAGAGGACCAGAAAGCTTTGCCCACTA
GTTCTAGACTGTGAGGAAGAGACTACATGAGAACTTAAAAAAAAAAAAAAGTTCTAGACTGTGAGGAAGAGACTACATGAGAACTTAAAAAAAAAAAAAA
Klon 0056-ContigClone 0056 contig
ATGCAATGGCGGACGTGTCTGAGAGGACGCTGCAGGTGTCCGTGCTAGTGGCTTTCGCCTCTGGAGTGGTCCTGGGCTGGATGCAATGGCGGACGTGTCTGAGAGGACGCTGCAGGTGTCCGTGCTAGTGGCTTTCGCCTCTGGAGTGGTCCTGGGCTGG
CAAGCGRATCGGCYGYGGAGGCGTTACCTAGACTGGAGGAAGCGGAGGCTGCAGGWCRRGCTGGCAACGACTCAGAAAAACAAGCGRATCGGCYGYGGAGGCGTTACCTAGACTGGAGGAAGCGGAGGCTGCAGGWCRRGCTGGCAACGACTCAGAAAAA
GCTGGACCTGGCCTGAGCACGCGCTGCAGCCCGAGTCCGCCGGGTTCTCACTCCCTAAGCCCAACGCAGCCCGGATCGTGGCTGGACCTGGCCTGAGCACGCGCTGCAGCCCGAGTCCGCCGGGTTCTCACTCCCTAAGCCCAACGCAGCCCGGATCGTG
GGAGCCGCGCGACCCAGGAGTCGTCCTTGCACGGCTTGCÄAGAACATGGCTTGCTTCAGAAAGAAÄATAGTTTTGTCTTCGGAGCCGCGCGACCCAGGAGTCGTCCTTGCACGGCTTGCÄAGAACATGGCTTGCTTCAGAAAGAAÄATAGTTTTGTCTTC
TCTAACAACTTACτTTCAGCTTGTCGAAGATGAAAATAAAAAGCACTGGAGAGÄÄATAATTTCTTGCACTTTATGAATCTTCTAACAACTTACτTTCAGCTTGTCGAAGATGAAAATAAAAAGCACTGGAGAGÄÄATAATTTCTTGCACTTTATGAATCT
ATTTTTAAAATAAAAAAATTAAACATCTTTGAATCTTTTTCCTCCTCACAAAAGAAAGCAGTATTTTTGCCTACCATTCAATTTTTAAAATAAAAAAATTAAACATCTTTGAATCTTTTTCCTCCTCACAAAAGAAAGCAGTATTTTTGCCTACCATTCA
GTTTGCTGCAGTAAGAGÄTTTGGAGCCTGAAAGCAGAGACTTTCTGATGGAATCTCACCTTGGTACÄGCCTGGAGGCAGAGTTTGCTGCAGTAAGAGÄTTTGGAGCCTGAAAGCAGAGACTTTCTGATGGAATCTCACCTTGGTACÄGCCTGGAGGCAGA
TCTGATCAACGGACCATTATGAGTCATTTTTCTAGACATATTCAGAAAACCTAGGAGCTGTGTCAAATGCCTGAATTAAG CATTACAAAΤGCAAGATATTTGCACTTTGAAGAATGTAGAGAGTAAAAAAACTAAAATTAAAAAAAATAATGCATGTGAT ATAACGGAATATATATGTGAAAGΆGAAAAAAAAAAAAAAAAAAAAAAATCTGATCAACGGACCATTATGAGTCATTTTTCTAGACATATTCAGAAAACCTAGGAGCTGTGTCAAATGCCTGAATTAAG C ATTACAA A Τ GCAAGATA TTT GC A CT T TGAAGAATGTAGAGAGTAAAAAAACTAAAATTAAAAAAAATAATGCATGTGAT ATAACGGAATATATATGTGAAAGΆGAAAAAAAAAAAAAAAAAAAAAAA
Klon 0057-ContigClone 0057 contig
AGATAGTTTTGAACTTCCGATTCCCCTGCCTCCCCACCCCAGGGCTGTGATTGCAGGTGTGCGCTTGGGACTGACCCCAG GCTTTGTCGGTGCTAGGGCAGCACTCTGCGACTGAATTAGGTCCCAGCCACTTCTCTGTCTTTTAAAAGAACAAΆACATT GCTAAATGTGCCATTGTTGCTTTGAGTTTTAATTCTTTTTTTTTTCTTTCTTTCATAAAΆCATTACAGTCTTAAGATATT AAAGACTTTTATTCTGGTTCTATTTCTGTCTTTTCACTCAAΆACTGGTTTTACAAATGATGCCTTGTTTACAGAAAGCTC TCTACCACAGGGCCTAGTCATGTGTAAAGTCTCAGTTTCTCTCTGGAGTATCTTGGAGCCTAGCACACTGGCTTTAAAGG ACACAGCTAAGAAGCTGATATCTTGACAGTGTTTGTAGACCTTTGTTATAAAAATGAATGTCCTGGAAAGGGTTGGGAGG GAGTTCAΆCAACAAAGAAACAAGAATGTCATGTTTAAATTTAATAGTTGTCTAAAATGTCATCTCAAGTCAΆGTCACTGG TCTGTTTGCATTTGATAGGTTTTTATACTAACTAGCATTATAAGATTATTTCATAATTAGAAAATACCTGTGGATATTTG TATAAAAGTGTGAAATAAATTTTTTACAAAAGTGCTCATCGCTTGTTAACACAGCATCATGTATGTGAAAGCAAACTCTA AGATTATAAATGACΆACCTGAGTTGCCTTTCTTTGTATTTCATCAΆGCCAAAGTAAAGCTTTCAATATTTAAAΆAAAAAΆ AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG ATAGTT T T GAAC T TCCG ATT CCCCTGCCTCCCCACCCCAGGGCTGTGATTGCAGGTGTGCGCTTGGGACTGACCCCAG GC TT TG T CGGTGCT A GGGC A GC ACT CTGCGACTGAATTAGGTCCCAGCCACTTCTCTGTCTTTTAAAAGAACAAΆACATT GC TAAAT GTGCCATTGTTGCTT T GAGTTTTAATTCTTTTTTTTTTCTTTCTTTCATAAAΆCATTACAGTCTTAAGATATT AA A GACTTT T ATTCTGGTTCTATTTCTGTCTTTTCACTCAAΆACTGGTTTTACAAATGATGCCTTGTTTACAGAAAGCTC T C TA CC A CAGGGCCTAGTCATGTGTAAAGTCTCAGTTTCTCTCTGGAGTATCTTGGAGCCTAGCACACTGGCTTTAAAGG AC A CAGCTAAGAAGCTGATATCTTGACAGTGTTTGTAGACCTTTGTTATAAAAATGAATGTCCTGGAAAGGGTTGGGAGG GAGTTCAΆCA A CAAAGAAACAAGAATGTCATGTTTAAATTTAATAGTTGTCTAAAATGTCATCTCAAGTCAΆGTCACTGG T C TGTTT GC ATT TGATAGGTTTTTATACTAACTAGCATTATAAGATTATTTCATAATTAGAAAATACCTGTGGATATTTG TATAAAA G T G T GA AA TAAATTTTTTACAAAAGTGCTCATCGCTTGTTAACACAGCATCATGTATGTGAAAGCAAACTCTA A G ATTATAAAT GAC ΆA CC T G A G TT GCCTTTCTTTGTATTTCATCAΆGCCAAAGTAAAGCTTTCAATATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Klon 0060-T7-SequenzClone 0060-T7 sequence
AGCATCCCTCCATGGCCTCTGCATTGGCTYCTGCTTTCTGACCTGCTTGAGTTCCAGTCCTGACTTCCTTGGTGATGAAC A GC ATC CC TC C A TGGCCTC T GC AT TGGCTYCTGCTTTCTGACCTGCTTGAGTTCCAGTCCTGACTTCCTTGGTGATGAAC
AGCAGTATGGAAGTGTAAGCCGAATAAACCCTTTCCTCTCCAACTTGTTTCTTGGTCATGATGTTTGTGCAGGAATAGAAAGCAGTATGGAAGTGTAAGCCGAATAAACCCTTTCCTCTCCAACTTGTTTCTTGGTCATGATGTTTGTGCAGGAATAGAA
ACCCTGACTAAGACAAATTGGTACCAGCΆGAGTGGGGTATTCCTGTGACAACCTGACCATGTTTTGGGGAGGACTGTGGA GGACTTTGGAACTTTGGGCTTAAAGATCCATCCGTTGTTAAGAGCTCTGTCAGATGTTGTGTAGGAGCTTGGAAGATAAACCCTGACTAAGACAAATTGGTACCAGCΆGAGTGGGGTATTCCTGTGACAACCTGACCATGTTTTGGGGAGGACTGTGGA GGACTTTGGAACTTTGGGCTTAAAGATCCATCCGTTGTTAAGAGCTCTGTCAGATAGGGAGGGG
TGTTGAGAACACTGCAGAAGATGGAGGTCTGGTTTGTGAAATTTCAGAGGGAAAATTAAAGACTCTTTTCAGGGCCATTGTGTTGAGAACACTGCAGAAGATGGAGGTCTGGTTTGTGAAATTTCAGAGGGAAAATTAAAGACTCTTTTCAGGGCCATTG
CTGTTTTGAATGTGAAGATTCTGTAGTTCTGGWTAGCTGGGGCTGAΆGAATCAGCTGTGATTAACAAGATACCAGAACTACTGTTTTGAATGTGAAGATTCTGTAGTTCTGGWTAGCTGGGGCTGAΆGAATCAGCTGTGATTAACAAGATACCAGAACTA
CCAAAGCAAAAACTTTGCATTACTGGGACTATTGATGCTGGTTAGCTGGAGCTAAGAAATTAGCGGTGATTAAGAAGAGACCAAAGCAAAAACTTTGCATTAC T GGGACTATTGATGCTGGTTAGCTGGAGCTAAGAAATTAGCGGTGATTAAGAAGAGA
CCAGCATCATTGAGGTGACATCTTCTGGGAAGTGTTTTCTGAAAGCACAAAGATGCTGTGTTCCAGAGATGGCCAAGGTTCCAGC A TCATTGAGGTGACATCTTCTGGGAAGTGTTTTCTGAAAGCACAAAGATGCTGTGTTCCAGAGATGGCCAAGGTT
GTACTCCTGCTGCAGCAGGACTTGGTAATATGTAAGGGTCACCCAGGTGGTACTGGTTTTGAAGGCATGAANGGGTCACGGTACTCCTGCTGCAGCAGGACTTGGTAATATGTAAGGGTCACCCAGGTGGTACTGGTTTTGAAGGCATGAANGGGTCACG
CAAAGCAGCTGANGCTCGGCACTGTGAGAGGCCATGGAAGGCCATTGCTGAAAGTACAGCCTCAGTTCAAAGCAGCTGANGCTCGGCACTGTGAGAGGCCATGGAAGGCCATTGCTGAAAGTACAGCCTCAGTT
Klon 0063-ContigClone 0063 contig
AAACGGCAACACTTCGGTCAGGATATCTTCTACCAGACACCAAGGCGTATGGAGATAGAATAGAAAGAATGCTTCGCCTC AGTTTAΆACATTGACCCTGAAGCACAGGTGGAGGAAGAACCAGAAGAAGAGCCTGAAGACACCTCAGAAGACGCAGAAGA CTCAGAGCAGGATGAGGGAGAAGAGATGGATGCAGGGACAGAAGAAGAAGAGGAGGAAACAGAAAAGGAATCTACAGAGA AGGATGAATTGTAAATTATACTCTCGCTATGAATCCCGTGTGGAGAGGGAATGTGAAGTTTTGAAGTCATTTCTTTTGAG AGACTTGTTTTGGATGCTTCCCCAAGCCYCCTTCTCCCCTGCMCTGTAAAATGTTGGGATTATGGGTCACAGGAARAAGT GGTTTTTTTAGTTGAATTTTTTTTAACATTCCTCCTGAATGTAAATTTGTACTATTTAACTGACTATTGGTGTAAAATCT TGTCATGTGTATAAAAATAAAAAAAGRTCCCCAATWAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAΆAAAAAAAAAA AAAAAAAAAAAAAAAAAAACGGCAACACTTCGGTCAGGATATCTTCTACCAGACACCAAGGCGTATGGAGATAGAATAGAAAGAATGCTTCGCCTC AGTTTAΆACATTGACCC T GA A GC A CAGGTGGAGGAAGAACCAGAAGAAGAGCCTGAAGACACCTCAGAAGACGCAGAAGA CTCAGAGCAGGATGAGGGAGAAGAGATGGATGCAGGGACAGAAGAAGAAGAGGAGGAAACAGAAAAGGAATCTACAGAGA AGGATGAATTGTAAATTATACTCTCGCTATGAATCCCGTGTGGAGAGGGAATGTGAAGTTTTGAAGTCATTTCTTTTGAG AGACTTGTTTTGGATGCTTCCCCAAGCCYCCTTCTCCCCTGCMCTGTAAAATGTTGGGATTATGGGTCACAGGAARAAGT GGTTTTTTTAGTTGAATTTTTTTTAACATTCCTCCTGAATGTAAATTTGTACTATTTAACTGACTATTGGTGTAAAATCT TGTCATGTGTATAAAAATAAAAAAAGRTCCCCAATWAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAΆAAAAAAAAAA AAAAAAAAAAAAAAAA
Klon 0065-ContigClone 0065 contig
AGGGGAGATCCTGAGAACAGACCAGTGGCCTCGGAGGCTCTTCATGCAGCTCATTCCGCAGCAGTTGCTGACCACCCTCG TGCCACTGTTCCGGAATTCACGCCTGGTACAGTTCCACTTCΆCTAAGGACATGGAGACTCTGAAGAGCCTTTGCCGGATC ATGGACAATGGCTTCGCGGGCTGCGTGCACTTCTCCTΆCAAGGCATCGTGTGAGGTGCGTGTGCTCATGCTCCTGTACTC CTCGGAGAAGAAGATCTTCATCGGCCTCATCCCGCACGACCAGAGCAACTTTGTCAACGGCATCCGTCGCGTCATCGCCA ACCAGCAGCAGGTCCTGCAGCGAAGCCTGGAGCAGGAGCAGCAGCAGCGAGGGATGGGTGGCTAGAGGATGCCTGGGCTG GGCGGGCCACAGTCCCAGACGAGGCCCCAGTGGAGACTGGTCAGCTGCTTCTGAGCAGGGGCTCCTGGCGACTGTACCTG CCCAGCAACCTGGAGGACAGCGTCCTGAGGTCTCCCAAGGATGGTCCTTGCCCCTGTATGTTTCCCTAATAAAGCCTTTT ACCCCAAAAAAAAAAAAAAAAAAAAAΆAAAGGGGAGATCCTGAGAACAGACCAGTGGCCTCGGAGGCTCTTCATGCAGCTCATTCCGCAGCAGTTGCTGACCACCCTCG TGCCACTGTTCCGGAATTCACGCCTGGTACAGTTCCACTTCΆCTAAGGACATGGAGACTCTGAAGAGCCTTTGCCGGATC ATGGACAATGGCTTCGCGGGCTGCGTGCACTTCTCCTΆCAAGGCATCGTGTGAGGTGCGTGTGCTCATGCTCCTGTACTC CTCGGAGAAGAAGATCTTCATCGGCCTCATCCCGCACGACCAGAGCAACTTTGTCAACGGCATCCGTCGCGTCATCGCCA ACCAGCAGCAGGTCCTGCAGCGAAGCCTGGAGCAGGAGCAGCAGCAGCGAGGGATGGGTGGCTAGAGGATGCCTGGGCTG GGCGGGCCACAGTCCCAGACGAGGCCCCAGTGGAGACTGGTCAGCTGCTTCTGAGCAGGGGCTCCTGGCGACTGTACCTG CCCAGCAACCTGGAGGACAGCGTCCTGAGGTCTCCCAAGGATGGTCCTTGCCCCTGTATGTTTCCCTAATAAAGCCTTTT ACCCCAAAAAAAAAAAAAAAAAAAAAΆAA
Klon 0066-ContigClone 0066 contig
GGCTGCTTCGTGGAGCAGAGAGGTGCATCACCAGGTTCCCGATGAACCCAGAGAACCCTCCACCGTATCCGGGCCCCGGG CCAACAGCCCCATACCCACCTTATCCACAACAGCCAATGGGGCCAATGGGGCCTATGGGAGCCCCACCTTCCTCAGGGGT ACCCCTACCCACCACCTCAGGGGTACCCCTATCAAGGATACCCACAGTACGGCTGGCAGGGTGGACCTCAGGAGCCTCCT AΆGACCACAGTGTÄTGTGGTGGAΆGACCAAΆCGAAGAGACGACCTGSGCCCATCCACCTGCCTCACAGCCTGCTGGACTG CTCTGTGTTGCTGCTGCCTCTGGGACATGCTCACCTGATCACCTGATGAGCCCAGCTCTTCCGCTTGGCCGCTCTGTGCC ACCTCCGATAAGTGTGCCTGGCCCCATCTCTTCTGATTGCTATAAAGTGGCTAGCTCTGCGCAGACACCTCTACTTTCTG TCCTATGGAGTTΤTAGATTAGTGAGGGTTACTGCTATTTAGTTCAGTGACTTGATCTTTTTAATGTTCAAAACTCATTTC TTACTGATCTTTAAAAAATGTGCTAAATAATTTACTTTTTTGGCCAAAGGCTTAGTTATGAAATATATATTTATAATGTT TAAATTATACACTCAAGGTAATGGCAATATGTGACACACTACTAGATCTCTCTGCTCATACCCTTTATGTTTCAATAAAT CTGTCCAAAATCTGGAAΆAAAAAAAAAAΆAAAAAAGGCTGCTTCGTGGAGCAGAGAGGTGCATCACCAGGTTCCCGATGAACCCAGAGAACCCTCCACCGTATCCGGGCCCCGGG CCAACAGCCCCATACCCACCTTATCCACAACAGCCAATGGGGCCAATGGGGCCTATGGGAGCCCCACCTTCCTCAGGGGT ACCCCTACCCACCACCTCAGGGGTACCCCTATCAAGGATACCCACAGTACGGCTGGCAGGGTGGACCTCAGGAGCCTCCT AΆGACCACAGTGTÄTGTGGTGGAΆGACCAAΆCGAAGAGACGACCTGSGCCCATCCACCTGCCTCACAGCCTGCTGGACTG CTCTGTGTTGCTGCTGCCTCTGGGACATGCTCACCTGATCACCTGATGAGCCCAGCTCTTCCGCTTGGCCGCTCTGTGCC ACCTCCGATAAGTGTGCCTGGCCCCATCTCTTCTGATTGCTATAAAGTGGCTAGCTCTGCGCAGACACCTCTACTTTCTG TCCTATGGAGTTΤTAGATTAGTGAGGGTTACTGCTATTTAGTTCAGTGACTTGATCTTTTTAATGTTCAAAACTCATTTC TTACTGATCTTTAAAAAATGTGCTAAATAATTTACTTTTTTGGCCAAAGGCTTAGTTATGAAATATATATTTATAATGTT TAAATTATACACTCAAGGTAATGGCA A TATGTGACACACTACTAGATCTCTCTGCTCATACCCTTTATGTTTCAATAAAT CTGTCCAAAATCTGGAAΆAAAAAAAA A AΆAAAAAA
Klon 0067-ContigClone 0067 contig
CTGGAAAGGCGCGGAGCCTGCTGCTGCCATGG GGCTGGTGGCTGGGTΆCACTATGTCTAGACTGGGGGCCCTGGGCGGC TCCCGCGCCGGGTTGGGACTGTTACTTGGTACTGCCGCCGGCCTTGGATTCCTGTGCGTCCTTTACAGCCAGCGATGGAΆ ACGGACCCAGCGCCATGGCCGGAGTCACAGTCTGCCCAACTCCCTGGACTATGCGCAGGCTTCAGAGCGTGGACGCCAGG TGACACAGTTTCGGGCTΆTCCCAGGTGAAGCTGGAGATGCTGCCATACTGCCCAGCCTCTCACAGGAAGGGYAGGAGAAG GTGCTGGACCGCCTGGACTTTGYGCTGACCAGTCTTATGGCGCTGCGGCGCGAGGTGGAGGAGCTTCAGAGAAGCCTGCA AGGACTAGCTGGGGAGATTGTCGGKGAGG CCGCTCTCATATAKAAGAGAACCAGAGAGWGGCCCGGCGGCGCASGTTCC CTTTTGCCAGAGAGAGGAGTGACTCCACGGGCTCCAGCTCTGTCTACTTCACCGCCTCCTCAGGGGCCGCACTCACAGAC GCCGAGAGCGAGGGAGGCTATACAACAGCCAACGCGGAGTCTGATTACGAGCGGGACTCCGACAAGGAGAGTGGAGATGC TGAGGACGAAGTGAGCTGCGΆGACCGTGAGGATGGGGAGGAAGGACTCTCTGGACCTGGATGTGGAGGCGGCGTCCAGTCCTGGAAAGGCGCGGAGCCTGCTGCTGCCATGG GGCTGGTGGCTGGGTΆCACTATGTCTAGACTGGGGGCCCTGGGCGGC TCCCGCGCCGGGTTGGGACTGTTACTTGGTACTGCCGCCGGCCTTGGATTCCTGTGCGTCCTTTACAGCCAGCGATGGAΆ ACGGACCCAGCGCCATGGCCGGAGTCACAGTCTGCCCAACTCCCTGGACTATGCGCAGGCTTCAGAGCGTGGACGCCAGG TGACACAGTTTCGGGCTΆTCCCAGGTGAAGCTGGAGATGCTGCCATACTGCCCAGCCTCTCACAGGAAGGGYAGGAGAAG GTGCTGGACCGCCTGGACTTTGYGCTGACCAGTCTTATGGCGCTGCGGCGCGAGGTGGAGGAGCTTCAGAGAAGCCTGCA AGGACTAGCTGGGGAGATTGTCGGKGAGG CCGCTCTCATATAKAAGAGAACCAGAGAGWGGCCCGGCGGCGCASGTTCC CTTTTGCCAGAGAGAGGAGTGACTCCACGGGCTCCAGCTCTGTCTACTTCACCGCCTCCTCAGGGGCCGCACTCACAGAC GCCGAGAGCGAGGGAGGCTATACAACAGCCAACGCGGAGTCTGATTACGAGCGGGACTCCGACAAGGAGAGTGGAGATGC TGAGGACGAAGTGAGCTGCGΆGACCGTGAGGATGGGGAGGAAGGACTCTCTGGACCTGGATGTGGAGGCGGCGTCCAGTC
CGTCCGTC
Klon 0068-ContigClone 0068 contig
AGCGCTGGGTCTGAGTGACCAAAGGCAGTAGCGCTCGCGGAGATCACCCGCTGGCCCTCGATCACCATGTCGGCCTTCGAAGCGCTGGGTCTGAGTGACCAAAGGCAGTAGCGCTCGCGGAGATCACCCGCTGGCCCTCGATCACCATGTCGGCCTTCGA
CACTAACCCCTTCGCGGACCCAGTGGACGTAAACCCCTTCCAGGATCCCTCTGTGACCCAGCTGACCAATGCTCCTCAGACACTAACCCCTTCGCGGACCCAGTGGACGTAAACCCCTTCCAGGATCCCTCTGTGACCCAGCTGACCAATGCTCCTCAGA
GTGGCCTGGCTGAGTTCAATCCCTTCTCAGAGACAAATGCAGCGACAACAGTTCCTGCCACACAAGCTCCTGGGCCCTCCGTGGCCTGGCTGAGTTCAATCCCTTCTCAGAGACAAATGCAGCGACAACAGTTCCTGCCACACAAGCTCCTGGGCCCTCC
CAGCCAGCAGTTCTCCAGCCCTCAGTGGAACCAGCACAGCCAACGCCCCAGGCTGTTGCAGCTGCGGCCCAGGCAGGCTTCAGCCAGCAGTTCTCCAGCCCTCAGTGGAACCAGCACAGCCAACGCCCCAGGCTGTTGCAGCTGCGGCCCAGGCAGGCTT
GCTTCGACAGCAGGAAGAACTAGACAGGAAAGCTGCCGAGCTGGAACGCAAGGAGCGAGAGTTGCAGAACACTGCAGCGAGCTTCGACAGCAGGAAGAACTAGACAGGAAAGCTGCCGAGCTGGAACGCAAGGAGCGAGAGTTGCAGAACACTGCAGCGA
ATTTGCATGTGCGAGACAACAACTGGCCGCCACTCCCCTCATGGTGCCCTGTGAAACCCTGCTTCTATCAGGACTTCTCCATTTGCATGTGCGAGACAACAACTGGCCGCCACTCCCCTCATGGTGCCCTGTGAAACCCTGCTTCTATCAGGACTTCTCC
ACGGAGATCCCTGCTGACTACCAGCGGATTTGCAAGATGCTTTACTATCTCTGGATGTTGCATTCAGYGACTCTGTTTCTACGGAGATCCCTGCTGACTACCAGCGGATTTGCAAGATGCTTTACTATCTCTGGATGTTGCATTCAGYGACTCTGTTTCT
AAACCTGCTTGCGTGCCTGGCCTGGTTCACAAGTGACGCAGCCAATGGAACAGCCTTTGGCCTCTCCATCCTTTGGTTTCAAACCTGCTTGCGTGCCTGGCCTGGTTCACAAGTGACGCAGCCAATGGAACAGCCTTTGGCCTCTCCATCCTTTGGTTTC
TGATCTTCACTCCCTGTGCCTTCCTTTGTTGGTACCGACCCATCTACAAGGCTTTCAGGTCTGACAACTCTTTCAGCTTCTGATCTTCACTCCCTGTGCCTTCCTTTGTTGGTACCGACCCATCTACAAGGCTTTCAGGTCTGACAACTCTTTCAGCTTC
TTCGTGTTCTTTTTTGTATTTTTTTGTCAAATAGGGATCTACTTCATCCAGTTGATTGGCTTGCCTAACTTGGGGACCAGTTCGTGTTCTTTTTTGTATTTTTTTGTCAAATAGGGATCTACTTCATCCAGTTGATTGGCTTGCCTAACTTGGGGACCAG
TGGTTGGCTTGCAGCCCTATCTACGATGAAAAATGGGCCCTTAGCTGTGACCATCATCATGATGGTAGTGGCTGGCTTCTTGGTTGGCTTGCAGCCCTATCTACGATGAAAAATGGGCCCTTAGCTGTGACCATCATCATGATGGTAGTGGCTGGCTTCT
TTACCCTCTGTGCTGGGCTTTCACTCTTCCTCCTGCAGCGGGTACACGCCTTCTACCGCCGAACAGGAGCCAGCTTCCAGTTACCCTCTGTGCTGGGCTTTCACTCTTCCTCCTGCAGCGGGTACACGCCTTCTACCGCCGAACAGGAGCCAGCTTCCAG
CAGGCCCAGGAAGAGTTCTCCCAGGGTATCTTCAGCAGCAGAACCTTTCGCGGGGCTGCCTCATCTGCTGCCCGAGGAGCCAGGCCCAGGAAGAGTTCTCCCAGGGTATCTTCAGCAGCAGAACCTTTCGCGGGGCTGCCTCATCTGCTGCCCGAGGAGC
CTTTCAGGGGAATTAGGCTTCCCGACTCTCCTTCCCTGAGCCCTGGCCTCTCTGTCAACTGTCTTCTGAGCTGCACTTTCCTTTCAGGGGAATTAGGCTTCCCGACTCTCCTTCCCTGAGCCCTGGCCTCTCTGTCAACTGTCTTCTGAGCTGCACTTTC
CTCGGGTGCCTTATGCAGTGACTACACCAGCACAGACCTGGCAGGGATTTGTCTTGTCCCTGCTCCTCCCTCAGTAGTCACTCGGGTGCCTTATGCAGTGACTACACCAGCACAGACCTGGCAGGGATTTGTCTTGTCCCTGCTCCTCCCTCAGTAGTCA
GCTTTCTCCTCTACAAGAGGGAAGGAGAGGAAGGGAGAAGGACTTTAAAÄAAAAAAAAAAAAAGCTTTCTCCTCTACAAGAGGGAAGGAGAGGAAGGGAGAAGGACTTTAAAÄAAAAAAAAAAAAA
Klon 0069-ContigClone 0069 contig
TTCGTCACTGCCAGGCTGAACTGGAGAGGCTTTCAGAGTCCCTGAGGAAGAGCATCTCCTGTGGAGCTTTCTCTGTTCCT GGGGGTCACAGCCTCTACTTAGAAGCCAGGAAGAAGATTGAGCTGGGCTACCAGCAAGTGCCGAGGAAGGGAGTGAAGGC AAAAGAGGTTCTCAAGAGTTTCCTACAGTCACAGGCTATTATGGAGGACTCTATCTTGCAGTCAGACAAAGCTCTTACAG ATGGAGAGAGAGCCATAGCAGCTGAGCGGACAAAGAAGGAAGTGGCTGAGAAGGAACTAGAGCTGCTGAGGCAGAGACAG AAGGAGCAAGAGCAAGTGATGGAGGCTCAGGAGAGAAGCTTCCGAGAAAACATTGCTAAACTTCAGGAGAAGATGGAGAG CGAAAAGGAGATGCTGCTGAGGGAGCAGGAGAAGATGCTGGAGCACAAGCTGAAGGTCCAAGAAGAACTGCTTATTGAAG GATTCAGAGAGAAATCTGATATGTTAAAGAATGAAATAAGTCACCTGAGAGAAGAGATGGAAAGAACAAGAAGGAAACCC TCACTGTTTGGTCAAATCCTTGACACCCGTTGGCAATGCGTTCATTATGATTTTACCAGGAGCTGGTAAACTATTTGGTG TGGGGCTGAAATTCCTCGGCTCACTAAGTAGTTAGTCTAAAGAGTTTCTGGGAAGGAATTTATAAAATCCAGGCTTCTGG TATTTTGATACCAAACTTTTTGCTGKGATTTTTAAGTACACATTCTAGTTTAATAACCACGGTTACMAAGAAAGTAAGAA CTTYCATTAATAGCTAΆATTGTCAGATACTGCTTCCTCGGAGTCTAATTCTGTGTCΆGTTCATTACCTATTATATTTAAG GTTÄAAGATNCAAACCAATGATTGAAGAATATAAGGGTCTGGCGTCCATAAΆTCCTGTCAAATTTCAGTGATGTTATCTA AATAATAAAGAGGGAGGCCAGTCAGAATATAGTGAAATTATTTCTAGAAAAGATGTAAGTAAATCAAACAGAAACATGGC CAAATGAAGACMCATTGGCATATGTATCCCAGTTGTACACTACAGATAAATTGTTAAAAAGAGCTCTTGTTAAAACGCMC AAGTGGGTTTTCATAAGAGAATGTATGAGGAAAATGTTTGATTGATAATAAAAGGCATGCAGATTAAAATAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAATTCGTCACTGCCAGGCTGAACTGGAGAGGCTTTCAGAGTCCCTGAGGAAGAGCATCTCCTGTGGAGCTTTCTCTGTTCCT GGGGGTCACAGCCTCTACTTAGAAGCCAGGAAGAAGATTGAGCTGGGCTACCAGCAAGTGCCGAGGAAGGGAGTGAAGGC AAAAGAGGTTCTCAAGAGTTTCCTACAGTCACAGGCTATTATGGAGGACTCTATCTTGCAGTCAGACAAAGCTCTTACAG ATGGAGAGAGAGCCATAGCAGCTGAGCGGACAAAGAAGGAAGTGGCTGAGAAGGAACTAGAGCTGCTGAGGCAGAGACAG AAGGAGCAAGAGCAAGTGATGGAGGCTCAGGAGAGAAGCTTCCGAGAAAACATTGCTAAACTTCAGGAGAAGATGGAGAG CGAAAAGGAGATGCTGCTGAGGGAGCAGGAGAAGATGCTGGAGCACAAGCTGAAGGTCCAAGAAGAACTGCTTATTGAAG GATTCAGAGAGAAATCTGATATGTTAAAGAATGAAATAAGTCACCTGAGAGAAGAGATGGAAAGAACAAGAAGGAAACCC TCACTGTTTGGTCAAATCCTTGACACCCGTTGGCAATGCGTTCATTATGATTTTACCAGGAGCTGGTAAACTATTTGGTG TGGGGCTGAAATTCCTCGGCTCACTAAGTAGTTAGTCTAAAGAGTTTCTGGGAAGGAATTTATAAAATCCAGGCTTCTGG TATTTTGATACCAA A C T G GC TTTTT K G A TTTTTAAGTACACATTCTAGTTTAATAACCACGGTTACMAAGAAAGTAAGAA CTTYCATTAATAGCTAΆATTGTCAG AT ACTGCTTCCTCGGAGTCTAATTCTGTGTCΆGTTCATTACCTATTATATTTAAG GTTÄAAGATNCAAACCAATGATTGAAGAATATAAGGGTCTGGCGTCCATAAΆTCCTGTCAAATTTCAGTGATGTTATCTA AATAATAAAG A GGGAGGCC A G T C A G AAT ATAGTGAAATTATTTCTAGAAAAGATGTAAGTAAATCAAACAGAAACATGGC CAAATGAAGACMCATTGGCATATGTATCCCAGTTGTACACTACAGATAAATTGTTAAAAAGAGCTCTTGTTAAAACGCMC AAGTGGGTTTTCATAAGAGAATGTATGAGGAAAATGTTTGATTGATAATAAAAGGCATGCAGATTAAAATAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Klon 0070-ContigClone 0070 contig
AGAACGCCTCTGGGGAGCATGGCATCGTGGTTTTCTCTTTGGGATCCATGGTCTCAGAGATTCCGGAGAAGAAAGCCATG GAAATTGCTGAGGCTTTGGGCAGAATTCCTCAGACGGTCCTGTGGCGCTACACCGGAACTAGACCATCGAATCTTGCAAA GAACACAATTCTTGTCAAATGGCTACCCCAAAATGATCTGCTTGGTCATCCAAAGACTCGGGCATTCATCACACACTCTG GCTCCCATGGTATTTATGAAGGAATATGCAATGGAGTTCCGATGGTGATGATGCCCCTATTTGGCGATCAGATGGACAAT GCCAAGCGCATGGAAACTCGGGGAGCTGGGGTGACCCTGAATGTCCTTGAAATGACTGCTGATGATTTGGAAAATGCCCT TAAAACTGTCATCAACAACAAGAGCTACAAGGAGAACATCATGCGCCTCTCCAGCCTTCACAAGGÄCCGTCCTATAGAGC CTCTGGACCTGGCTGTGTTCTGGGTGGAATACGTGATGAGGCACAAGGGGGCACCACACCTGCGCCCGGCCGCCCATGAC CTCACCTGGTATCAGTACCACTCCTTGGATGTGATTGGCTTCCTCCTGGCCATTGTGTTGACAGTGGTCTTCATTGTCTT TAAATGTTGTGCCTATGGCTGCCGGAΆATGCTTTGGGGGAAAGGGGCGAGTGAAGAAATCACACAAATCCAAGACCCATT GAGAAGTGGGGGGAAGTGAAGGAGAAGTATTAGTTCATTATCTGATCAGTTGAACYTTGGAAACAΆGTGTTAGATCCATA TTGTTTTTGTTAGGGAAATAATTCACCATACATTATACATTCAGCACATTTAAAAATAATAATAATAACAATCTAATTGC TGGCCACACCCATCAGGGAAGGTTCTAGTATATGTGATGTGCTTTTCCAGTATCTTCAGTCTAGACAACTCTTGCCATCT GTTGGTAATTTACAGAAAGTCTGGCACTCTGCTTTCAGTGACAGCCCCACAGTTTCCCCTGCTCCCGCCAGCTGATGGTT TCTCCCCTGGATTCTCAGACTGCCGTGGCCTTCTCCAGTGTTAGTCATTCTTCATTGTGTTCATGCATTATGGGTAGCA AGACCTTTGGAGCTTTGGGAGAAGAGATGAGGCTGTGACACTGATGGCCCTGTGTTTGAGATAATAATTGTTGCTTGTGC CGGAATTTGATGAAAACCAAAGTATGTTCTAAGGCAAGTACATCTTCTATTGTGTTCCCAAACCAAGAΆCTTATCΆATAA ATTCATATAAATTGTATCACTAAAAAAAAAAAAAAAAAAGAACGCCTCTGGGGAGCATGGCATCGTGGTTTTCTCTTTGGGATCCATGGTCTCAGAGATTCCGGAGAAGAAAGCCATG GAAATTGCTGAGGCTTTGGGCAGAA T TCCTCAGACGGTCCTGTGGCGCTACACCGGAACTAGACCATCGAATCTTGCAAA GAACACAATTCTTGTCAAATGGCTACCCCAAAATGATCTGCTTGGTCATCCAAAGACTCGGGCATTCATCACACACTCTG GCTCCCATGGTATTTATGAAGGAATATGCAATGGAGTTCCGATGGTGATGATGCCCCTATTTGGCGATCAGATGGACAAT GCCAAGCGCATGGAAAC T CGGGGAGC T GGGGTGACCCTGAATGTCCTTGAAATGACTGCTGATGATTTGGAAAATGCCCT TAAAACTGTCATCAACAACAAGAGCTACAAGGAGAACATCATGCGCCTCTCCAGCCTTCACAAGGÄCCGTCCTATAGAGC CTCTGGACCTGGCTGTGTTCTGGGTGGAATACGTGATGAGGCACAAGGGGGCACCACACCTGCGCCCGGCCGCCCATGAC CTCACCTGGTATCAGTACCACTCCTTGGATGTGATTGGCTTCCTCCTGGCCATTGTGTTGACAGTGGTCTTCATTGTCTT TAAATGTTGTGCCTATGGCTGCCGGAΆATGCTTTGGGGGAAAGGGGCGAGTGAAGAAATCACACAAATCCAAGACCCATT GAGAAGTGGGGGGAAGTGAAGGAGAAGTATTAGTTCATTATCTGATCAGTTGAACYTTGGAAACAΆGTGTTAGATCCATA TTGTTTTTGTTAGGGAAATAATTCACCATACATTATACATTCAGCACATTTAAAAATAATAATAATAACAATCTAATTGC TGGCCACACCCATCAGGGAAGGTTCTAGTATATGTGATGTGCTTTTCCAGTATCTTCAGTCTAGACAACTCTTGCCATCT GTTGGTAATTTACAGAAAGTCTGGCACTCTGCTTTCAGTGACAGCCCCACAGTTTCCCCTGCTCCCGCCAGCTGATGGTT TCTCCCCTGGATTCTCAGACTGCCGTGGCCTTCTCCAGTGTTAGTCATTCTTCATTGTGTTCATGCATTATGGGTAGCA AGACCTTTGGAGCTTTGGGAGAAGAGATGAGGCTGTGACACTGATGGCCCTGTGTTTGAGATAATAATTGTTGCTTGTGC CGGAATTTGATGAAAACCAAAGTATGTTCTAAGGCAAGTACATCTTCTATTGTGTTCCCAAACCAAGAΆCTTATCΆATAA ATTCATATAAATTGTATCACTAAAAAAAAAAAAAAAAA
Klon 0071-T7-SequenzClone 0071-T7 sequence
AGAGAAGCAGACATGGCTCAATTTGCACAGGTCATGGCTGAAGTGGGCGACTTTGGTCGCTTTCAGGTGCGGTTGACCAT CCTGATGGGCATTCCCAATTTCCTGGCTGCATTCTTCATATTTGGCCAAGTCTTCATGGTCCTTGATGAGGCTCACCACT GTTCAGTGTCCTGGGTTAAGAACCACACTTTCAACCTAAGTGCCGCTGAGCAGCTGGCTATAAGCATCCCCAACGACACA GCGGGCAGACCCGAGTCCTGCCTCATGTTCCGGCCACCTCCTGACAGTGCCAGCCTGGAAGACATGCTGAGCCACCGCTT CAATGAGACACAGGCCTGTGACTCAGGCTGGGACTATCCTGAGAACCGGCCTCAGTCCCTAAAGAAAGAGTTTGACCTGG TGTGTGATCGAAAGAACCTGAAGAAGACCTCCCAGTCGGTGTTCATGGCTGGTCTCCTTGTTGGGGCCCTGGTCTTTGGG CCTGTCTGTGACTGGATTGGCCGCAGACCCTCCCTCCTGATGCAGGTGCTTCTGTCAGGCATCACAAGCATGGCCACAGC CTTCGTGTCCAGCTTTGAGCTCTACTTGGCCCTACGCTTTGTCTTGGCTACTGCCAATGCTGGATTTTTACTAAGTACCA ATGTCCTGATTTCAGAGTGGGTGGGGCCΆTCTTGGAGAACACAAGCCGTGGTCTTTGGCCCANAGCAΆCGTTGCCCTTGG GCAGATGGTGTTAGCAGGACTGGCCTATGGTGTCCGAAACTTGGAGACTTCTTCAGATACANGGACCCGACCCGCTTACT GNTCTTCTTCTATTTCTAGAGAAGCAGACATGGCTCAATTTGCACAGGTCATGGCTGAAGTGGGCGACTTTGGTCGCTTTCAGGTGCGGTTGACCAT CCTGATGGGCATTCCCAATTTCCTGGCTGCATTCTTCATATTTGGCCAAGTCTTCATGGTCCTTGATGAGGCTCACCACT GTTCAGTGTCCTGGGTTAAGAACCACACTTTCAACCTAAGTGCCGCTGAGCAGCTGGCTATAAGCATCCCCAACGACACA GCGGGCAGACCCGAGTCCTGCCTCATGTTCCGGCCACCTCCTGACAGTGCCAGCCTGGAAGACATGCTGAGCCACCGCTT CAATGAGACACAGGCCTGTGACTCAGGCTGGGACTATCCTGAGAACCGGCCTCAGTCCCTAAAGAAAGAGTTTGACCTGG TGTGTGATCGAAAGAACCTGAAGAAGACCTCCCAGTCGGTGTTCATGGCTGGTCTCCTTGTTGGGGCCCTGGTCTTTGGG CCTGTCTGTGACTGGATTGGCCGCAGACCCTCCCTCCTGATGCAGGTGCTTCTGTCAGGCATCACAAGCATGGCCACAGC CTTCGTGTCCAGCTTTGAGCTCTACTTGGCCCTACGCTTTGTCTTGGCTACTGCCAATGCTGGATTTTTACTAAGTACCA ATGTCCTGATTTCAGAGTGGGTGGGGCCΆTCTTGGAGAACACAAGCCGTGGTCTTTGGCCCANAGCAΆCGTTGCCCTTGG GCAGATGGTGTTAGCAGGACTGGCCTATGGTGTCCGAAACTTGGAGACTTCTTCAGATACANGGACCCGACCCGCTTACT GNTCTTCTTCTATTTCT
Klon 0073-T7-SequenzClone 0073-T7 sequence
AGCGGGCCTGAGCCGAGCAGCTGCGCGACGTCATGGACAACTCCGGGAAGCAGGCTGAGGCTATGGCGCTGCTGGCTGAAAGCGGGCCTGAGCCGAGCAGCTGCGCGACGTCATGGACAACTCCGGGAAGCAGGCTGAGGCTATGGCGCTGCTGGCTGAA
GCGGAGCGCAAGGTGAAGAACTCGCAGTCCTTCTTCTCCGGCCTCTTTGGAGGCTCATCCAAAATAGAGGAAGCATGCGAGCGGAGCGCAAGGTGAAGAACTCGCAGTCCTTCTTCTCCGGCCTCTTTGGAGGCTCATCCAAAATAGAGGAAGCATGCGA
GATCTATGCCAGAGCGGCGAACATGTTCAAGATGGCCAAGAACTGGAGCGCTGCTGGGAACGCTTTCTGCCAGGCTGCCCGATCTATGCCAGAGCGGCGAACATGTTCAAGATGGCCAAGAACTGGAGCGCTGCTGGGAACGCTTTCTGCCAGGCTGCCC
AACTACACCTACAGCTCCAAÄGCAAGCACGATGCAGCCACCTGCTTTGTGGACGCTGGCAATGCTTTCAAGAAAGCTGACAACTACACCTACAGCTCCAAÄGCAAGCACGATGCAGCCACCTGCTTTGTGGACGCTGGCAATGCTTTCAAGAAAGCTGAC
CCCCAAGAGGCCATTAACTGTCTGATGAGAGCAATTGAGATCTATACAGACATGGGCAGATTCACAATCGCAGCCAAGCACCCCAAGAGGCCATTAACTGTCTGATGAGAGCAATTGAGATCTATACAGACATGGGCAGATTCACAATCGCAGCCAAGCA
CCACATCTCCATCGCTGAGATCTATGAGACAGAACTGGTGGATGTAGAGAAGGCCATCGCCCACTATGAGCAATCCTGCACCACATCTCCATCGCTGAGATCTATGAGACAGAACTGGTGGATGTAGAGAAGGCCATCGCCCACTATGAGCAATCCTGCA
GACTACTACAAAAGGGAGAAGAGTCCAACAGCTNAGCCAACAAGYGTCTGCTGAAGGTGGCTGGCTACGCCCGAACAGCTGACTACTACAAAAGGGAGAAGAGTCCAACAGCTNAGCCAACAAGYGTCTGCTGAAGGTGGCTGGCTACGCCCGAACAGCT
GGAGCAGTACCAGAAGGCTATCGACATCTATTGAGCAGGGGGGGACCAGTGCCGGAGCAGTACCAGAAGGCTATCGACATCTATTGAGCAGGGGGGGACCAGTGCC
Klon 0074-T7-SequenzClone 0074-T7 sequence
AAAGTTGTTCTTCTCGTGGTTCCCAGTGGCGAGAGGAGGAGGAAGCCCGGAGCGGAGCGGGGCGKCTGGGGGGGGTGGACAAAGTTGTTCTTCTCGTGGTTCCCAGTGGCGAGAGGAGGAGGAAGCCCGGAGCGGAGCGGGGCGKCTGGGGGGGGTGGAC
CCGCCGCGGCTGCTGCTGCCACCGCCGCCGCCGCCACCACCGCTCGTGGGGCTCGTGGCGTGÄGGAAGGAGGACGAGTGACCGCCGCGGCTGCTGCTGCCACCGCCGCCGCCGCCACCACCGCTCGTGGGGCTCGTGGCGTGÄGGAAGGAGGACGAGTGA
GACCCCGGGGCGAGCGGGCGGCGGCGCCGCTGCTGCTGCTGCTGCTGCGGGAGGGTCGGCGGCGGGACGGCGATGGCGGAGACCCCGGGGCGAGCGGGCGGCGGCGCCGCTGCTGCTGCTGCTGCTGCGGGAGGGTCGGCGGCGGGACGGCGATGGCGGA
TATCGACAAACTCAACATCGACAGCATCATCCAACGGCTGCTGGAAGTGAGAGGGTCCAAGCCAGGCAAGAATGTCCAGCTATCGACAAACTCAACATCGACAGCATCATCCAACGGCTGCTGGAAGTGAGAGGGTCCAAGCCAGGCAAGAATGTCCAGC
TCCAGGAGAACGAGATCCGAGGACTCTGCCTGAAGTCTCGGGAGATCTTCCTCAGTCAGCCTATCCTTTTAGAACTTGAATCCAGGAGAACGAGATCCGAGGACTCTGCCTGAAGTCTCGGGAGATCTTCCTCAGTCAGCCTATCCTTTTAGAACTTGAA
GCACCACTCAAGATATGTGGNGACATCCACGGGCAGTACTATGATTTGCTCCGTCTGTTTGAATACGGTGGCTTTCCTCCGCACCACTCAAGATATGTGGNGACATCCACGGGCAGTACTATGATTTGCTCCGTCTGTTTGAATACGGTGGCTTTCCTCC
AGAGAGCAACTATTTGTTTCTCGGGGACTATGTGGACAGGGGCAAGCAGTCCCTGGAGACAATCTGCCTCTTGCTGGCCTAGAGAGCAACTATTTGTTTCTCGGGGACTATGTGGACAGGGGCAAGCAGTCCCTGGAGACAATCTGCCTCTTGCTGGCCT
ACAAAATCAAGTATCCGGAGAACTTCTTTCTTCTCAGAGGGAACCACGAGTGCGCCAGCATCAATAGGATCTACSGATTTACAAAATCAAGTATCCGGAGAACTTCTTTCTTCTCAGAGGGAACCACGAGTGCGCCAGCATCAATAGGATCTACSGATTT
TATGATGAGTGTAAAAGAAGATACAMCATTAAGCTTGTGGAAAACGTTCACAGACTGKTTTAACTGCTTGCCGATAGCAATATGATGAGTGTAAAAGAAGATACAMCATTAAGCTTGTGGAAAACGTTCACAGACTGKTTTAACTGCTTGCCGATAGCAA
GCCATCGTGGACNAAGAAGATAWTCTGCTGTCATGGAGGGTTATCACCAGAWCTTCAATCTATGGAGCAGAATTCSGCSGGCCATCGTGGACNAAGAAGATAWTCTGCTGTCATGGAGGGTTATCACCAGAWCTTCAATCTATGGAGCAGAATTCSGCSG
AAATATKAGACCAAYTGATGTACCAGAWCAAGGGCCTTCTTTKGGAACCNTTGGGGGCCTGANCCCCATAAAGAGGCNTTAAATATKAGACCAAYTGATGTACCAGAWCAAGGGCCTTCTTTKGGAACCNTTGGGGGCCTGANCCCCATAAAGAGGCNTT
AAGCTTGGGTGGAAAATGCCCGAGGAGNGGCCTTCCAATTGGTGCCAAATAAGCTTGGGTGGAAAATGCCCGAGGAGNGGCCTTCCAATTGGTGCCAAAT
Klon 0076-ContigClone 0076 contig
AGGATCATCCGTCTCGCCTCCAGTTCTTCACCGGCTGAGCTGGGTACGGACATCTCAGTGACCACGAGCTAACATAGCACAGGATCATCCGTCTCGCCTCCAGTTCTTCACCGGCTGAGCTGGGTACGGACATCTCAGTGACCACGAGCTAACATAGCAC
GTGAAACTGACCTCAGGAAGAACGAAGCCTGGAGACCCGGAGTGCGACAGCGACCTCCGGACTGAAGTGGAAGAAGCGTAGTGAAACTGACCTCAGGAAGAACGAAGCCTGGAGACCCGGAGTGCGACAGCGACCTCCGGACTGAAGTGGAAGAAGCGTA
GCGAGGCCTTCGTGTCCTCGCTGTGGGGGGCGGGGCTTGTTGTGCAGGCGGGGCTCACGTGGTACTTTGGTACTTTGGATGCGAGGCCTTCGTGTCCTCGCTGTGGGGGGCGGGGCTTGTTGTGCAGGCGGGGCTCACGTGGTACTTTGGTACTTTGGAT
GACTGAAGGGÄAGCTTGGTACCCGCCTGGTCACTACCCGGAAGTCCAGCCGGAGTAGCACTACCCTGGAGTCCACCCGGCGACTGAAGGGÄAGCTTGGTACCCGCCTGGTCACTACCCGGAAGTCCAGCCGGAGTAGCACTACCCTGGAGTCCACCCGGC
TGTGAAGCGGTGGTGCTCTTAGGGGTGCTTGACGCCCTCATTGTTAGAAACTGATGGGTACTGTTGGCCGATTCCTAGACTGTGAAGCGGTGGTGCTCTTAGGGGTGCTTGACGCCCTCATTGTTAGAAACTGATGGGTACTGTTGGCCGATTCCTAGAC
CCGCTTTTGACCTACGTGATAAATGTGTTTCCGGAACTCTGCCAAAAAGTGTTCTGGAGGTTTTTGTTTGTTTCGGAGCCCCGCTTTTGACCTACGTGATAAATGTGTTTCCGGAACTCTGCCAAAAAGTGTTCTGGAGGTTTTTGTTTGTTTCGGAGCC
AAGGCCTCCCTCGTAGCTCTTAATGGCCTGAAACACTCTTTGTAGCCTAGACTGGCATTGAAGTCAAGTTAGCCCTGCCTAAGGCCTCCCTCGTAGCTCTTAATGGCCTGAAACACTCTTTGTAGCCTAGACTGGCATTGAAGTCAAGTTAGCCCTGCCT
TTGCCTCCCTGGGCTCTGGGATTACCCTGCCTTTCTTGATTATTTCTATTTGAACAAATATGAACAAACTATGTGGCCACTTGCCTCCCTGGGCTCTGGGATTACCCTGCCTTTCTTGATTATTTCTATTTGAACAAATATGAACAAACTATGTGGCCAC
CACAAAAAAAAAAAAAAAAAAAAAAAAA Klon 0077-ContigCACAAAAAAAAAAAAAAAAAAAAAAAAA Clone 0077 contig
AGATGGAGCCTGCGTTTCACAGAGGGGATCTCCTTTTCCTCACGAACCGAGTTGAAGATCCTATACGCGTGGGGGAGATC GTTGTTTTCAGGATAGAAGGAAGAGAGATTCCTATAGTGCACCGAGTCCTGAAGATCCATGAAAAGCAAGATGGGCATAT CAAGTTTTTAACCAAAGGAGATAATAATGCTGTTGATGACCGAGGTCTCTATAAACAAGGACAACACTGGCTGGAGAAGA AAGATGTTGTGGGGAGAGCAAGAGGGTTTGTTCCTTACATTGGAATTGTGACGATCCTCATGAATGACTATCCTAAATTT AAGTATGCAGTACTGTTTCTGCTCGGTTTATTTGTGCTGGTCCATCGTGAGTAAGAAGTCGGACTCCCTGTTCCTΆGGAA GCTGCTGTGCTTGTTGTTACTGAATGTTGGAGTAGATCCTGATCTGTGATTGCGGATTTTCGGAGGACACACACGTTGGC ACTTCTTGGTAGCCCTGGTTTGCATTGCTTTGTGTTTCCACACCAGAGGCTGTGTGGGCGGGTGCATGTGCACCGTGGAG TGCACACAAGGGGACTGTCAATCACAGGGTTTCATATGTTGTCATTGTCACTCTTTCACATTTTTGTACATCΆGTGAATT TTTTATATTAAAAGGTTGAGCCAAAGCCCCCAGTGTTTGTATTTTGAAGCCAAGCTTCACTTTAAAΆGTGCCTACAGAGT TCTGTAAATGAAAACACAGCTCTGCATGAGTTCAAACCTGTCGKTCCTYCTTACAGTAGGAATGGCACATATTGAGGCGG KCATAAGTCTTAACTTTTCAAAATTTTAΆATAAAAGACTTTGCACΆTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAGATGGAGCCTGCGTTTCACAGAGGGGATCTCCTTTTCCTCACGAACCGAGTTGAAGATCCTATACGCGTGGGGGAGATC GTTGTTTTCAGGATAGAAGGAAGAGAGATTCCTATAGTGCACCGAGTCCTGAAGATCCATGAAAAGCAAGATGGGCATAT CAAGTTTTTAACCAAAGGAGATAATAATGCTGTTGATGACCGAGGTCTCTATAAACAAGGACAACACTGGCTGGAGAAGA AAGATGTTGTGGGGAGAGCAAGAGGGTTTGTTCCTTACATTGGAATTGTGACGATCCTCATGAATGACTATCCTAAATTT AAGTATGCAGTACTGTTTCTGCTCGGTTTATTTGTGCTGGTCCATCGTGAGTAAGAAGTCGGACTCCCTGTTCCTΆGGAA GCTGCTGTGCTTGTTGTTACTGAATGTTGGAGTAGATCCTGATCTGTGATTGCGGATTTTCGGAGGACACACACGTTGGC ACTTCTTGGTAGCCCTGGTTTGCATTGCTTTGTGTTTCCACACCAGAGGCTGTGTGGGCGGGTGCATGTGCACCGTGGAG TGCACACAAGGGGACTGTCAATCACAGGGTTTCATATGTTGTCATTGTCACTCTTTCACATTTTTGTACATCΆGTGAATT TTTTATATTAAAAGGTTGAGCCAAAGCCCCCAGTGTTTGTATTTTGAAGCCAAGCTTCACTTTAAAΆGTGCCTACAGAGT TCTGTAAATGAAAACACAGCTCTGCATGAGTTCAAACCTGTCGKTCCTYCTTACAGTAGGAATGGCACATATTGAGGCGG KCATAAGTCTTAACTTTTCAAAATTTTAΆATAAAAGACTTTGCACΆTTTAAAAAAAAAAAAAAAAAAAAAAAAAAA
Klon 0079-ContigClone 0079 contig
AAAGTTCTTCTCTCACGTGGGTTGGCTGCTTGTGCGCAAACACCCGGCTGTCAAAGAGAAGGGCGGAAAACTGGACATGT CTGACCTGAAAGCCGAGAAGCTGGTGATGTTCCAGAGGAGGTACTACAAGCCCGGCCTCCTGCTGATGTGCTTCATCCTG CCCACGCTGGTGCCCTGGKACTGCTGGGGCGAGACTTTTGTAAACAGCCTGTTCGTTAGCACCTTCTTGCGATACACTCT GGTGCTCAAACGCCACCTGGCTGGTGAACAGTGCCGCGCATCTCTATGGATATCGCCCCTACGACAAGAACATTCAATCC CGGGAGAAATATCCTGGKTTCCCTGGGTGCCGTGGGCGAGNGGCTTCCACAACTACCACCACACCTTYCCCTTCGACTAC TCTGCCAGTGAGTACCGCTGGCACATCAACTTCACCACGTTCTTCATCGACTGCATGGCTGCCCTGGGCCTGGCTTACGA CCGGAAGAAAGTTTCTAARGCTACTGTCTTAGCCAGGATTAAGAGAACTGGAGACGGGAGTCACAAGAGTAGCTGAGCTTAAAGTTCTTCTCTCACGTGGGTTGGCTGCTTGTGCGCAAACACCCGGCTGTCAAAGAGAAGGGCGGAAAACTGGACATGT CTGACCTGAAAGCCGAGAAGCTGGTGATGTTCCAGAGGAGGTACTACAAGCCCGGCCTCCTGCTGATGTGCTTCATCCTG CCCACGCTGGTGCCCTGGKACTGCTGGGGCGAGACTTTTGTAAACAGCCTGTTCGTTAGCACCTTCTTGCGATACACTCT GGTGCTCAAACGCCACCTGGCTGGTGAACAGTGCCGCGCATCTCTATGGATATCGCCCCTACGACAAGAACATTCAATCC CGGGAGAAATATCCTGGKTTCCCTGGGTGCCGTGGGCGAGNGGCTTCCACAACTACCACCACACCTTYCCCTTCGACTAC TCTGCCAGTGAGTACCGCTGGCACATCAACTTCACCACGTTCTTCATCGACTGCATGGCTGCCCTGGGCCTGGCTTACGA CCGGAAGAAAGTTTCTAARGCTACTGTCTTAGCCAGGATTAAGAGAACTGGAGACGGGAGTCACAAGAGTAGCTGAGCTT
TGGGCTTYTGAGWTCCTGTTTYAAACGKTTTCTGGCAGAGATTTAATATTCTGTTGATTAACTAACAACTGGATATTGCT ATCGGGGTGTTAATGATGCATTTAACCTATTCCGGKACAGTATTCTTATAAAATGAGAAAGCTTTGATCACGTTTTGAGG TTGGGCTTYTGAGWTCCTGTTTYAAACGKTTTCTGGCAGAGATTTAATATTCTGTTGATTAACTAACAACTGGATATTGCT ATCGGGGTGTTAATGATGCATTTAACCTATTCCGGKACAGTATTCTTATAAAATGAGAAAGGTTTGT
Klon 0080-T7-SequenzClone 0080-T7 sequence
TCATACAAACACCCCAGTACCTCCAGCAGCTCCAGGAGTCAAAAACACCAGCAGTGGACCGAGGTCCTACAGCTGCCTGCTCATACAAACACCCCAGTACCTCCAGCAGCTCCAGGAGTCAAAAACACCAGCAGTGGACCGAGGTCCTACAGCTGCCTGC
AGCCATGTTGGCCTTCAGTCTGCTCGTGCTGGGTCTGCTCGCGGAAGTGGCACCTGCCAGCTGTCAACAAGGTCTAGGAAAGCCATGTTGGCCTTCAGTCTGCTCGTGCTGGGTCTGCTCGCGGAAGTGGCACCTGCCAGCTGTCAACAAGGTCTAGGAA
ACCTTCAGCCCTGGATGCAAGGCCTCATTGCTGTCGCYGTGTTCTTGGTCCTYGTTGCAATCGYCTTYGC GTCAACCACACCTTCAGCCCTGGATGCAAGGCCTCATTGCTGTCGCYGTGTTCTTGGTCCTYGTTGCAATCGYCTTYGC GTCAACCAC
TTCTGGTGCCAGGAGGAGCCGGAGCCTGSGAGCACAGTSMTGAYCRTYGGAAACAAGGCAGATGGRGTCCTGGTGGGAAYTTCTGGTGCCAGGAGGAGCCGGAGCCTGSGAGCACAGTSMTGAYCRTYGGAAACAAGGCAGATGGRGTCCTGGTGGGAAY
RGATGGMAGRTACTCYTCRATGGCRKCYRGTTTYAGGTCCAGYGAGCAYRAGAATGCCTAYGAGAATGTKCYSGAGGARGRGATGGMAGRTACTCYTCRATGGCRKCYRGTTTYAGGTCCAGYGAGCAYRAGAATGCCTAYGAGAATGTKCYSGAGGARG
ARGGCARGGTCCGCAGCACMCCSATGTGRCRAGCYKCTMYSYRK CS GTCCCCAGRCTACGCWTAGAGTTGATCCCCAARGGCARGGTCCGCAGCACMCCSATGTGRCRAGCYKCTMYSYRK CS GTCCCCAGRCTACGCWTAGAGTTGATCCCCA
CCATCTCACCCAGCCACTGCTCTACAGGAATCTACTGAAACAAGCTGACTYCCTCACCTCTCTAGAATCACAGTCATCTYCCATCTCACCCAGCCACTGCTCTACAGGAATCTACTGAAACAAGCTGACTYCCTCACCTCTCTAGAATCACAGTCATCTY
AGGCCAGGACTCAGACCAAGCCCAGCACACCAATGTGCTGTGACCCYKGGCTTTCCAGGCCAGGACTCAGACCAAGCCCAGCACACCAATGTGCTGTGACCCYKGGCTTTCC
Klon 0081-ContigClone 0081 contig
AAAGCGCTCCTCGAGGGTCGGTCCCGCCACGTCTCTTCCCGGTCTCTCTGGCTCTGGAGTGCTTTCCCCCCGCGGAGGGTAAAGCGCTCCTCGAGGGTCGGTCCCGCCACGTCTCTTCCCGGTCTCTCTGGCTCTGGAGTGCTTTCCCCCCGCGGAGGGT
GGGAGCGCGGGGACAGACGGGCGAGATGAGCACCATGTTTGCAGACACGCTGCTCATCGTCTTTATCTCCGTGTGCACCGGGGAGCGCGGGGACAGACGGGCGAGATGAGCACCATGTTTGCAGACACGCTGCTCATCGTCTTTATCTCCGTGTGCACCG
CGCTGCTCGCCGAGGGCATAACCTGGGTCCTGGTTTACAGGACAGACAAATACAAGAGATTGAAGGCAGAAGTGGAAAAACGCTGCTCGCCGAGGGCATAACCTGGGTCCTGGTTTACAGGACAGACAAATACAAGAGATTGAAGGCAGAAGTGGAAAAA
CAAAGTAAAAAATTAGAGAAGAAGAAGGAAACTATAACAGAGTCAGCTGGTCGACAACAGAAGAAGAAGATAGAGAGACACAAAGTAAAAAATTAGAGAAGAAGAAGGAAACTATAACAGAGTCAGCTGGTCGACAACAGAAGAAGAAGATAGAGAGACA
AGAAGAGAAATTAAAGAACAACAATAGAGACCTGTCAATGGTACGGATGAAATCCATGTTCGCGATTGGCTTTTGTTTTAAGAAGAGAAATTAAAGAACAACAATAGAGACCTGTCAATGGTACGGATGAAATCCATGTTCGCGATTGGCTTTTGTTTTA
CTGCCTTAATGGGAATGTTTAATTCCATATTTGATGGTAGAGTGGTGGCAAAGCTCCCCTTCACTCCTCTGTCTTACATCCTGCCTTAATGGGAATGTTTAATTCCATATTTGATGGTAGAGTGGTGGCAAAGCTCCCCTTCACTCCTCTGTCTTACATC
CAAGGACTATCTCATCGGAACCTGCTGGGAGATGACACCACGGACTGCTCCTTCATCTTCCTGTATATCCTCTGTACCATCAAGGACTATCTCATCGGAACCTGCTGGGAGATGACACCACGGACTGCTCCTTCATCTTCCTGTATATCCTCTGTACCAT
GTCAATTCGACAAAACATCCAGAAGATTCTTGGCCTCGCCCCTTCACGAGCTGCCACCAAGCAGGCTGGTGGATTTCTTGGTCAATTCGACAAAACATCCAGAAGATTCTTGGCCTCGCCCCTTCACGAGCTGCCACCAAGCAGGCTGGTGGATTTCTTG
GCCCACCACCTYCGTCTGGGAAGTTTTCCTGAAGGAAAGCAGAATTCTGAATTTCCTGTCATACTTTTTAGACATTCACAGCCCACCACCTYCGTCTGGGAAGTTTTCCTGAAGGAAAGCAGAATTCTGAATTTCCTGTCATACTTTTTAGACATTCACA
TCAGACTTACMGAGCACCTGGCCACAWTCTAGGTNKGKGTWATTTCTCAGCTATGGATATGA CMAATGAGAACCCTGMTTCAGACTTACMGAGCACCTGGCCACAWTCTAGGTNKGKGTWATTTCTCAGCTATGGATATGA CMAATGAGAACCCTGMT
TTAACTAA GGGA ARTGCTATGGTCACCGGATGGCTTCTTTNAGTAATAAGTGS CYCNNTCTGGKTACCATTTGGA GTTAACTAA GGGA ARTGCTATGGTCACCGGATGGCTTCTTTNAGTAATAAGTGS CYCNNTCTGGKTACCATTTGGA G
GCTTAATGTAACCCCMAACCATCAATCTTTCTTGTCTYACTTGAGTATTTCAGTGTATGTGCCATATACACAGAAGCAGAGCTTAATGTAACCCCMAACCATCAATCTTTCTTGTCTYACTTGAGTATTTCAGTGTATGTGCCATATACACAGAAGCAGA
CTGTCCATGAACATTAGGAGAAAAATAGTTTATGTAACAACTTCACTGTAATTAGTTGTTGTGTTTTTTAAAAAGCCAAACTGTCCATGAACATTAGGAGAAAAATAGTTTATGTAACAACTTCACTGTAATTAGTTGTTGTGTTTTTTAAAAAGCCAAA
TATGTCACTTTGTGTTCGACAATGAGCCAAATTTTTAGATGTTTCAGTTAGAGAAATGTTTGATTTTTTTGCATATTAAATATGTCACTTTGTGTTCGACAATGAGCCAAATTTTTAGATGTTTCAGTTAGAGAAATGTTTGATTTTTTTGCATATTAAA
AAGAGTATTTTCTGTAATCTGAACATAAGGATAAGCATTGGCCCAAAAAGCTAAAATAAAGTTTTGTCATGTTCTTTTACAAGAGTATTTTCTGTAATCTGAACATAAGGATAAGCATTGGCCCAAAAAGCTAAAATAAAGTTTTGTCATGTTCTTTTAC
CAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAA
Klon 0082-ContigClone 0082 contig
AGGCGACATGGGGAGGCGCGGAGGCGACACCGGAAGTGGCTGTGGTCCGGGTCGGCCCGAGGGGGACTCAGCGCCCGCAGAGGCGACATGGGGAGGCGCGGAGGCGACACCGGAAGTGGCTGTGGTCCGGGTCGGCCCGAGGGGGACTCAGCGCCCGCAG
CAACCACCCGCGCGGCGGCGGTCAGAGCTCATACCCGTGCGGGTGGGCGTGGCGGGCGCCGGGACACAGCTCCGACTGTTCAACCACCCGCGCGGCGGCGGTCAGAGCTCATACCCGTGCGGGTGGGCGTGGCGGGCGCCGGGACACAGCTCCGACTGTT
CGCTGTCGGCGCGGRGMGKCGCCGCGTCGCTCGCCTTNACCAGCTGCCATGAGCGAGCGCCTCCGCCCCAGAAAAAGGAGCGCTGTCGGCGCGGRGMGKCGCCGCGTCGCTCGCCTTNACCAGCTGCCATGAGCGAGCGCCTCCGCCCCAGAAAAAGGAG
AAGAAATGGCAGTGATGATGACAACCACCCTCCTCCCCAGACCAAAAGGAGCAGTAGGAACCCCATCTTCCAGGACTCCTAAGAAATGGCAGTGATGATGACAACCACCCTCCTCCCCAGACCAAAAGGAGCAGTAGGAACCCCATCTTCCAGGACTCCT
GGGACACAGAGTCTTCAAGCAGCGACAGTGGTGGGAGCAGCAGCAGCAGCAGCATCAACAGCCCAGACAGGGCCAGTGGG CCAGAGAGCAGCCTGAGCCACACCATCCCCGGATCCTGCCCCAGCACCCCCCAGCCGATGCCTGAGCAGTCTGCACTATG CCAAGGCCCTTACTTCCACATCAACCAGACCCTGAAGGAGGCTCACTTTCATAGCCTACAGCACCGAGGCCGGCCGCCGA CATGATGCTCCTCCGGCAGTTTCTTGCCTTCTGTGAAGGGACAGCACTGTGCAGATTGGÄTATTTCAACTTAATGATTTG TT TTAAAAGTTGCACACAGAAAAAAAAAAAAAAAAAGGGACACAGAGTCTTCAAGCAGCGACAGTGGTGGGAGCAGCAGCAGCAGCAGCATCAACAGCCCAGACAGGGCCAGTGGG CCAGAGAGCAGCCTGAGCCACACCATCCCCGGATCCTGCCCCAGCACCCCCCAGCCGATGCCTGAGCAGTCTGCACTATG CCAAGGCCCTTACTTCCACATCAACCAGACCCTGAAGGAGGCTCACTTTCATAGCCTACAGCACCGAGGCCGGCCGCCGA CATGATGCTCCTCCGGCAGTTTCTTGCCTTCTGTGAAGGGACAGCACTGTGCAGATTGGÄTATTTCAACTTAATGATTTG TT TTAAAAGTTGCACACAGAAAAAAAAAAAAAAAAA
Klon 0083-ContigClone 0083 contig
TGCTGAGGTGGGAGTGGGCCCAGCGCCCGGTGGCCGCAGCTCACGCGCAACCTGCGCCATGGCCGCCTCCGCCGCCTCCT CCGAGCATTTCGAGAAGCTGCACGAGATCTTCCGCGGCCTCCTTGAAGACTTACAÄGGGGTGCCGGAGCGGCTGCTGGGG ACCGCGGGGACAGAAGAGAAGAAGAAGCTGGTCAGAGATTTTGATGAAAAGCAACAGGAAGCAAATGAAACGTTGGCAGA GATGGAGGAAGAACTACGATΆTGCACCCCTGACTTTCCGTAACCCCΆTGATGTCTAAGCTGCGAAACTACCGGAAGGACC TTGCTAΆACTCCACCGTGAGGTGAGAAGTACACCTCTGACAGCCGCACCTGGAGGCCGΆGGAGACCTGAAGTATGGCACG TATGCCTTGGAGAÄCGAGCATTTGAATCGACTACAGTCTCAAAGAGCATTACTCCTACAAGGCACTGAAAGCCTGAACCG GGCTACCCAAAGCATTGAGCGTTCTCATCGGATTGCCACAGAAACTGATCAAATTGGTACAGAAATCATAGAAGAGTTGK GGGAGCAACGAGACCAGTTGGAACGTACTAAGAGCAGACTGGTAAATACAAATGΆAAATTTGAGCAAAAGCCGGAAGATT CTTCGCTCAATGTCCAGAAAAGTGATAACCAACAAGTTGCTGCTCTCCGTCATCATCTTGCTGGAGCTAGCCATCCTGGT CGGTCTGGTGTATTACAAATTCTTTCGACACCATTGAACTTCTGTAGGGAAAGCCTTGTGGACCAGCATCTTACTCTGAA TGAATGGTTTTACATAGGGATATGTGTTGCTGCTGTGGGCTAACAGTTCAAGAATGTATTGTCTTGTCAGACTATGGGAG GGAGGGCAATGGAAAΆCCTCGAAAATGTGAAGGAACAGCAACAAGACCAGTATGATATACTAAGGTAATAAATGTTGTTT ATGACTCCTTCAAACTTAAAAAAAAAAAAAAAAAAAAAAAAATGCTGAGGTGGGAGTGGGCCCAGCGCCCGGTGGCCGCAGCTCACGCGCAACCTGCGCCATGGCCGCCTCCGCCGCCTCCT CCGAGCATTTCGAGAAGCTGCACGAGATCTTCCGCGGCCTCCTTGAAGACTTACAÄGGGGTGCCGGAGCGGCTGCTGGGG ACCGCGGGGACAGAAGAGAAGAAGAAGCTGGTCAGAGATTTTGATGAAAAGCAACAGGAAGCAAATGAAACGTTGGCAGA GATGGAGGAAGAACTACGATΆTGCACCCCTGACTTTCCGTAACCCCΆTGATGTCTAAGCTGCGAAACTACCGGAAGGACC TTGCTAΆACTCCACCGTGAGGTGAGAAGTACACCTCTGACAGCCGCACCTGGAGGCCGΆGGAGACCTGAAGTATGGCACG TATGCCTTGGAGAÄCGAGCATTTGAATCGACTACAGTCTCAAAGAGCATTACTCCTACAAGGCACTGAAAGCCTGAACCG GGCTACCCAAAGCATTGAGCGTTCTCATCGGATTGCCACAGAAACTGATCAAATTGGTACAGAAATCATAGAAGAGTTGK GGGAGCAACGAGACCAGTTGGAACGTACTAAGAGCAGACTGGTAAATACAAATGΆAAATTTGAGCAAAAGCCGGAAGATT CTTCGCTCAATGTCCAGAAAAGTGATAACCAACAAGTTGCTGCTCTCCGTCATCATCTTGCTGGAGCTAGCCATCCTGGT CGGTCTGGTGTATTACAAATTCTTTCGACACCATTGAACTTCTGTAGGGAAAGCCTTGTGGACCAGCATCTTACTCTGAA TGAATGGTTTTACATAGGGATATGTGTTGCTGCTGTGGGCTAACAGTTCAAGAATGTATTGTCTTGTCAGACTATGGGAG GGAGGGCAATGGAAAΆCCTCGAAAATGTGAAGGAACAGCAACAAGACCAGTATGATATACTAAGGTAATAAATGTTGTTT ATGACTCCTTCAAACTTAAA ÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁ
Klon 0084-ContigClone 0084 contig
CTTTTTTTTTTTTTTTTTTTTCCGTTATAATAGGCATCTTTATTTGTAAAAATCCAGATATAAAACGTAATCTTTCAGTCCTTTTTTTTTTTTTTTTTTTTCCGTTATAATAGGCATCTTTATTTGTAAAAATCCAGATATAAAACGTAATCTTTCAGTC
TTTCCAGGTTTTCCTTTTTTACAAAAACAAAAAGGCACGTATAAACCTTGCCCGCTGTCGTCCCCGTACACGGTGTTTCTTTTCCAGGTTTTCCTTTTTTACAAAAACAAAAAGGCACGTATAAACCTTGCCCGCTGTCGTCCCCGTACACGGTGTTTCT
CAGGCAGCCCTCCCCCCCGCCCCGCCCCCCGTTACAGCTACATGCTTCATTCCAGGACGTCTGCATCCCCACATGCTTTGCAGGCAGCCCTCCCCCCCGCCCCGCCCCCCGTTACAGCTACATGCTTCATTCCAGGACGTCTGCATCCCCACATGCTTTG
GTGCTTTCCTACCAGGGTAGAGTTCCGAGCTCCAAGACTTGAAGTACACAAAGAGGGGGTAGGGGTGGGTGCAGTGTGTGGTGCTTTCCTACCAGGGTAGAGTTCCGAGCTCCAAGACTTGAAGTACACAAAGAGGGGGTAGGGGTGGGTGCAGTGTGTG
GCACAATGTTCCACGGCGTGCAGGGCAGTGGGCTAGTAGTAGGTCTCCTTCTCCACCCAGCCGCCAGGGCGCCGCCTGATGCACAATGTTCCACGGCGTGCAGGGCAGTGGGCTAGTAGTAGGTCTCCTTCTCCACCCAGCCGCCAGGGCGCCGCCTGAT
AATGAGCTTCCGCACCTCGTCATACACAAAGATGAGAAGGGAGTAGGGGAAGGCACAGAACCACCATGTÄGGTTTGAGGGAATGAGCTTCCGCACCTCGTCATACACAAAGATGAGAAGGGAGTAGGGGAAGGCACAGAACCACCATGTÄGGTTTGAGGG
GATACATCCTAARGGCTGCCCCCATCCCGGGGCAGTAGGATAAGAAAGCAGCAAGGGCTGTCTCTTCAAAGAGGCCAAATGATACATCCTAARGGCTGCCCCCATCCCGGGGCAGTAGGATAAGAAAGCAGCAAGGGCTGTCTCTTCAAAGAGGCCAAAT
ATCAAGATCTTGKTCTTCATTCCCTGCTGGAAGACAGAATTCCTTCTGGTCTTGCAGATGACCAAGTCGGCCCACTGCACATCAAGATCTTGKTCTTCATTCCCTGCTGGAAGACAGAATTCCTTCTGGTCTTGCAGATGACCAAGTCGGCCCACTGCAC
TACCACAATACTGACAAAGAACGCTGTATGGCAGGTGAACTCCACGATCTTCCTCTGCTCGTAGGTCCACTGCTGCCCGTTACCACAATACTGACAAAGAACGCTGTATGGCAGGTGAACTCCACGATCTTCCTCTGCTCGTAGGTCCACTGCTGCCCGT
AGCTGTCCTCCACATCGTTGACCCAGCGGTCATCCCAGGTCTCTCGGATGCCCAACAGGTGAAAGGGCAGGAAACCGTTCAGCTGTCCTCCACATCGTTGACCCAGCGGTCATCCCAGGTCTCTCGGATGCCCAACAGGTGAAAGGGCAGGAAACCGTTC
TCAGCCAGAATCACAAAGTAAGTGAAGAAGCCTCCCAGGGCCTGGATCATACCGATCTGTCCATAGGCCATGCTGATCAGTCAGCCAGAATCACAAAGTAAGTGAAGAAGCCTCCCAGGGCCTGGATCATACCGATCTGTCCATAGGCCATGCTGATCAG
ACGCTCGTTCACAAGTTTGTCCGTTTTGGGGTTTCTGGGCTGCCTCTTCATGATGTCGCTCTCAGCTTGCTCGTAGGCCAACGCTCGTTCACAAGTTTGTCCGTTTTGGGGTTTCTGGGCTGCCTCTTCATGATGTCGCTCTCAGCTTGCTCGTAGGCCA
GGGAGATGGCAGGAACCATGTCAGTGCCCAAGTCAATGCAGAGGATGGTCACAGTCCCCAGGGGCAGTGGAATGTTTGCAGGGAGATGGCAGGAACCATGTCAGTGCCCAAGTCAATGCAGAGGATGGTCACAGTCCCCAGGGGCAGTGGAATGTTTGCA
ATAATAAATATCAAGAAGGGGGTGATTTCCGGAATGTTACTGGTTAGGGTGTAAGCGATGGATTTCTTCAAGTTATCAAAATAATAAATATCAAGAAGGGGGTGATTTCCGGAATGTTACTGGTTAGGGTGTAAGCGATGGATTTCTTCAAGTTATCAAA
AATCAGACGACCTTCCTCTACTCCAGTCACAATGGAGGCAAAGTTGTCATCCAGAAGGATCATGTCAGCAGCTTGCTTGGAATCAGACGACCTTCCTCTACTCCAGTCACAATGGAGGCAAAGTTGTCATCCAGAAGGATCATGTCAGCAGCTTGCTTGG
ACACATCAGAGCCAACAATCCCCAACACATCAGAGCCAACAATCCCCA
Klon 0085-ContigClone 0085 contig
AGGTCTGCGGCCCTCGCAGAACTTCCAGCAGCGACATGTTGGGCCAGAGTATCCGGAGGTTCACGACCTCCGTGGTCCGT CGCAGCCACTATGAGGAGGGTCCGGGGAAGAATTTGCCATTTTCAGTGGAAAACAAGTGGCGGTTGCTGGCTATGATGAC CGTGTACTTTGGATCTGGGTTTGCCGCACCTTTCTTTATAGTAAGACACCAGCTACTTAAAAAATAAGGATATTTAATTC ATCCCTTTAACAGAΆTGAAGAAAGTTTAAGAGGTGATCTGAAAATTGGATTAAACTCTTGAACTCTTATACTAGAAAAAA TTGTAAATAAACTAATGACATAAAGATTCAAAAAAAAAAAAAAAAAAAAAAGGTCTGCGGCCCTCGCAGAACTTCCAGCAGCGACATGTTGGGCCAGAGTATCCGGAGGTTCACGACCTCCGTGGTCCGT CGCAGCCACTATGAGGAGGGTCCGGGGAAGAATTTGCCATTTTCAGTGGAAAACAAGTGGCGGTTGCTGGCTATGATGAC CGTGTACTTTGGATCTGGGTTTGCCGCACCTTTCTTTATAGTAAGACACCAGCTACTTAAAAAATAAGGATATTTAATTC ATCCCTTTAACAGAΆTGAAGAAAGTTTAAGAGGTGATCTGAAAATTGGATTAAACTCTTGAACTCTTATACTAGAAAAAA TTGTAAATAAACTAATGACATAAAGATTCAAAAAAAAAAAAAAAAAAAAA
Klon 0086-T7-SequenzClone 0086 T7 sequence
AGATGAATTCAAAGAGTGCCCAGGGTCTGGCTGGTCTTCGAAACCTTGGGAACACGTGCTTCATGAACTCAATTCTTCAGAGATGAATTCAAAGAGTGCCCAGGGTCTGGCTGGTCTTCGAAACCTTGGGAACACGTGCTTCATGAACTCAATTCTTCAG
TGCCTGAGCAACACCCGAGAGCTGAGAGATTACTGCCTCCAGAGGCTGTACATGCGGGACCTCGGCCACACCAGCAGCGCTGCCTGAGCAACACCCGAGAGCTGAGAGATTACTGCCTCCAGAGGCTGTACATGCGGGACCTCGGCCACACCAGCAGCGC
TCACACGGCCCTCATGGAAGAGTTTGCAAAACTAATCCAGACCATATGGACGTCGTCCCCCAATGATGTGGTGAGCCCATTCACACGGCCCTCATGGAAGAGTTTGCAAAACTAATCCAGACCATATGGACGTCGTCCCCCAATGATGTGGTGAGCCCAT
CTGAGTTCAAGACCCAGATCCAGAGATATGCGCCACGCTTCATGGGCTATAATCAGCAGGATGCTCAGGAATTCCTTCGTCTGAGTTCAAGACCCAGATCCAGAGATATGCGCCACGCTTCATGGGCTATAATCAGCAGGATGCTCAGGAATTCCTTCGT
TTCCTTCTGGATGGTCTCCACAATGAGGTGAACCGGGTGGCAGCAAGGCCTAAGGCCAGCCCTGAGACCCTTGATCATCTTTCCTTCTGGATGGTCTCCACAATGAGGTGAACCGGGTGGCAGCAAGGCCTAAGGCCAGCCCTGAGACCCTTGATCATCT
CCCTGATGAAGAAAAGGGGCGACAGATGTGGAGGAAGTATCTGGAAAGGGAAGACAGTCGGATTGGGGATCTCTTCGTTGCCCTGATGAAGAAAAGGGGCGACAGATGTGGAGGAAGTATCTGGAAAGGGAAGACAGTCGGATTGGGGATCTCTTCGTTG
GGCAGCTGAAGAGCTCCCTCACATGCACCGATTGTGGCTACTGCTCTACAGTCTTCGATCCCTTCTGGGATCTCTCGTTGGGCAGCTGAAGAGCTCCCTCACATGCACCGATTGTGGCTACTGCTCTACAGTCTTCGATCCCTTCTGGGATCTCTCGTTG
CCCATCGCAAAGAGAGGTTACCCTGAGGTGACGTTAATGGATTGTATGAGGCTCTTCACCAAASAGGACATATTGGATGGCCCATCGCAAAGAGAGGTTACCCTGAGGTGACGTTAATGGATTGTATGAGGCTCTTCACCAAASAGGACATATTGGATGG
GTGATGAGAAGCCAACTTGCTGCCGCTGCCGAGCCAGAAAACGATGCATAAAAAAGNTCTCTGTCCAGAGGGTGATGAGAAGCCAACTTGCTGCCGCTGCCGAGCCAGAAAACGATGCATAAAAAAGNTCTCTGTCCAGAGG
Klon 0087-Contig AGGGCGCCTGAGTGAAAAGTGTGGCACCATGGCCTCTGTGCTGTCCTACGAAAGTCTGGTACACGCCGTGGCCGGAGCCG TGGGAAGTGTGACTGCCATGACAGTGTTCTTCCCCTTGGATACTGCTAGACTTCGGCTTCAGGTCGATGAGAAAAGAAAG TCAAAAACGACGCATGCAGTGCTCCTGGAGATAATTAAGGAAGAAGGCCTCCTGGCACCATACCGAGGATGGTTTCCAGY TATTTCCAGTCTCTGCTGCTCCAATTTTGTCTATTTTTACACTTTTAATAGCCTCAAAGCAGTGTGGGTCAAAGGTCAGC GTTCTTCTACAGGAAAAGATCTCGTGGTTGGGTTTGTAGCAGGAGTGGTGAATGTGCTGCTGACGACTCCGCTCTGGGTG GTAAACACCAGACTGAAGCTGCAGGGGGCAAAATTTCGGAATGAAGMCATTATACCARCTAACTACAAAGGCATTATCGA TGCATTCCACCAGATTATTCGAGATGAAGGGATCTTGGCTCTGTGGAATGGCACCTTCCCCTCCTTGCTGWTGG CTTCA ACCCTGCCATCCAATTCATGTTCTATGAAGGCTTAAAACGGCAGCTTCTAAAGAANCGGATGAÄGCTCTCTTCTCTGNAT GTGTTCATCATTGGCGCAATAGCCANAGCGATTGCCNCCNCAGTCACCTATCCCATGCAGACGGTACAGTCAÄTTCTGAG GTTTGGACGTCATAGACTGAACCCAGAAAACAGGACCCTGGGAAGTCTTCGGAATGTTCTCTCTCTTCTTCACCAGCGAG TCAAGCGCTTTGGAATAATGGGACTCTACAAAGGCCTGGAAGCTAAGCTGCTGCAGACAGTGCTCACAGCCGCCCTCATG TTCCTTGTGTACGAGAAACTGACAGCTGCTACCTTCNCCGTAATGGGCCTGAAGAGCACACATAAGCNCTGAGGCCCGCC CNCAGGACAGTGTGGAGACGCAGGTGCTGCACAGGGAGAAGAGGAACAGTTCTCCTTTCCCGTTGGTCCCATCCNCNCCN CAGACATTCCAGTCATTGGCTGTAAAGGCATCAAAGGCTAAGCAGGGAGTATAGCCAGCTAAGCCTGTCACCTTTATGGG GTTTTTTGGTGTTTTTATGGTGTTTGGTTGTATTTGGGGGTAGAGGTTAGACTGATATGAAAATATTGAAACCCTGAAAA AAAAAAAAAAAClone 0087 contig AGGGCGCCTGAGTGAAAAGTGTGGCACCATGGCCTCTGTGCTGTCCTACGAAAGTCTGGTACACGCCGTGGCCGGAGCCG TGGGAAGTGTGACTGCCATGACAGTGTTCTTCCCCTTGGATACTGCTAGACTTCGGCTTCAGGTCGATGAGAAAAGAAAG TCAAAAACGACGCATGCAGTGCTCCTGGAGATAATTAAGGAAGAAGGCCTCCTGGCACCATACCGAGGATGGTTTCCAGY TATTTCCAGTCTCTGCTGCTCCAATTTTGTCTATTTTTACACTTTTAATAGCCTCAAAGCAGTGTGGGTCAAAGGTCAGC GTTCTTCTACAGGAAAAGATCTCGTGGTTGGGTTTGTAGCAGGAGTGGTGAATGTGCTGCTGACGACTCCGCTCTGGGTG GTAAACACCAGACTGAAGCTGCAGGGGGCAAAATTTCGGAATGAAGMCATTATACCARCTAACTACAAAGGCATTATCGA TGCATTCCACCAGATTATTCGAGATGAAGGGATCTTGGCTCTGTGGAATGGCACCTTCCCCTCCTTGCTGWTGG CTTCA ACCCTGCCATCCAATTCATGTTCTATGAAGGCTTAAAACGGCAGCTTCTAAAGAANCGGATGAÄGCTCTCTTCTCTGNAT GTGTTCATCATTGGCGCAATAGCCANAGCGATTGCCNCCNCAGTCACCTATCCCATGCAGACGGTACAGTCAÄTTCTGAG GTTTGGACGTCATAGACTGAACCCAGAAAACAGGACCCTGGGAAGTCTTCGGAATGTTCTCTCTCTTCTTCACCAGCGAG TCAAGCGCTTTGGAATAATGGGACTCTACAAAGGCCTGGAAGCTAAGCTGCTGCAGACAGTGCTCACAGCCGCCCTCATG TTCCTTGTGTACGAGAAACTGACAGCTGCTACCTTCNCCGTAATGGGCCTGAAGAGCACACATAAGCNCTGAGGCCCGCC CNCAGGACAGTGTGGAGACGCAGGTG CTGCACAGGGAGAAGAGGAACAGTTCTCCTTTCCCGTTGGTCCCATCCNCNCCN CAGACATTCCAGTCATTGGCTGTAAAGGCATCAAAGGCTAAGCAGGGAGTATAGCCAGCTAAGCCTGTCACCTTTATGGG GTTTTTTGGTGTTTTTATGGTGTTTGGTTGTATTTGGGGGTAGAGGTTAGACTGATATGAAAATATTGAAACCCTGAAAA AAAAAAAAAAA
Klon 0088-ContigClone 0088 contig
AGTAGAGCCCCTACGGTCATGGCGGCTACCGCCCCCAAGGCCGGGGGTTCAGCTCCTGAGGCAGCGGGTTCTGCCGAAGCAGTAGAGCCCCTACGGTCATGGCGGCTACCGCCCCCAAGGCCGGGGGTTCAGCTCCTGAGGCAGCGGGTTCTGCCGAAGC
TCCGTTGCAGTACAGCCTTCTTCTTCAGTACCTGG GGGCGACAAGCGTCAGCCCCGGCTTCTGGAGCCTGGCAGCCTGGTCCGTTGCAGTACAGCCTTCTTCTTCAGTACCTGG GGGCGACAAGCGTCAGCCCCGGCTTCTGGAGCCTGGCAGCCTGG
GCGGGATCCCGAGTCCTGCCAAGAGCGAGGAGCAGAAGATGATCGAGAGGGCAATGGAAAGTTGCGCCTTCAAGGCTG GGCGGGATCCCGAGTCCTGCCAAGAGCGAGGAGCAGAAGATGATCGAGAGGGCAATGGAAAGTTGCGCCTTCAAGGCTG G
CTGGCCTGTGTAGGAGGGTTTGTCTTGGGAGGCGCGTTTGGTATCTTTACTGCTGGCATTGATACCAACGTAGGCTTTGACTGGCCTGTGTAGGAGGGTTTGTCTTGGGAGGCGCGTTTGGTATCTTTACTGCTGGCATTGATACCAACGTAGGCTTTGA
CCCAAAGGACCCTTACCGGACACCAACTGCAAAAGAAGTCCTGAAAGACATGGGACAGAGAGGAATGTCCTATGCCAAAACCCAAAGGACCCTTACCGGACACCAACTGCAAAAGAAGTCCTGAAAGACATGGGACAGAGAGGAATGTCCTATGCCAAAA
ACTTTGCCATTG GGGCGCCATGTTTTCATGTÄCTRAGTGTCTGGTAGAGTCTTACCGGGGAAAGTCGGRCTGGAAGAACACTTTGCCATTG GGGCGCCATGTTTTCATGTÄCTRAGTGTCTGGTAGAGTCTTACCGGGGAAAGTCGGRCTGGAAGAAC
AGCGTCATCAGTGGCTGCATCACTGGCGGAGCCATCGGCTTCCGAGCTGGAGTAAAGGCCGGGGCCATAGGTTGTGGAGGAGCGTCATCAGTGGCTGCATCACTGGCGGAGCCATCGGCTTCCGAGCTGGAGTAAAGGCCGGGGCCATAGGTTGTGGAGG
GTTTGCTGCTTTCTCTGCTGCAATCGATTATTACCTACGGTGAAGGAANCAGCCGGGAAGGANAGAGGACGCCAGCCNCCGTTTGCTGCTTTCTCTGCTGCAATCGATTATTACCTACGGTGAAGGAANCAGCCGGGAAGGANAGAGGACGCCAGCCNCC
TTCANAGCTGCTCTGTGGANCAGTTTCTACACACTTGGACTGCTTCAGGGGTGAAGCCAAGTGCATCCCTCTACAGTTNATTCANAGCTGCTCTGTGGANCAGTTTCTACACACTTGGACTGCTTCAGGGGTGAAGCCAAGTGCATCCCTCTACAGTTNA
TGGTGTNAAGGACNCNCCTCGCTGCTCGATCGCTCTCTGCAGCCNTTGGAACAACCATACTTTTCCMCTTGAATTCCAACTGGTGTNAAGGACNCNCCTCGCTGCTCGATCGCTCTCTGCAGCCNTTGGAACAACCATACTTTTCCMCTTGAATTCCAAC
CAGGTTTTTCAGGGRAGGTGGTATGGCAGATTGTCTCCTTGCAGTAAMCAGATCAGGAGSCAGTGTGTGGMCTGTTACGC TTAAGCTTTGAACCAGGCTCAGTTCTGMCCTGGCTCAGCTCCCCAGCTTTGAATAGGGAAGTGSCAGTGTTTGCCCATACAGGTTTTTCAGGGRAGGTGGTATGGCAGATTGTCTCCTTGCAGTAAMCAGATCAGGAGSCAGTGTGTGGMCTGTTACGC TTAAGCTTTGAACCAGGCTCAGTTCTGMCCTGGCTCAGCTCCCCAGCTTTTGAATAGGATAAG
SCCGGGAAGCTCTTCTTCCTTCTGTGTGCAAGTATCCATCATGAGTCCCAGGGCAGGSCAAAGTAAACTTATGCCATCGASCCGGGAAGCTCTTCTTCCTTCTGTGTGCAAGTATCCATCATGAGTCCCAGGGCAGGSCAAAGTAAACTTATGCCATCGA
GGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Klon 0089-T7-SequenzClone 0089-T7 sequence
AAGGCCGGGAGGATGCGGCCGGAGCCCGGAGGCTGCTGCTGCCGCCGCCCGATGCGGGCGAACGGCTGCGTCAAGAACGGAAGGCCGGGAGGATGCGGCCGGAGCCCGGAGGCTGCTGCTGCCGCCGCCCGATGCGGGCGAACGGCTGCGTCAAGAACGG
GGAAGTGAGGAACGGGTACTTGAGGAGCAGCACCGCCACCGTCGCGGCTGCCGGCCAGATTCATCATGTAACAGAAAATGGGAAGTGAGGAACGGGTACTTGAGGAGCAGCACCGCCACCGTCGCGGCTGCCGGCCAGATTCATCATGTAACAGAAAATG
GAGGACTGTACAAAÄGACCGTTTAATGAAGCTTTTGAAGAAACACCCATGCTGGYTGCTGTGCTCACATATGTGGGCTATGAGGACTGTACAAAÄGACCGTTTAATGAAGCTTTTGAAGAAACACCCATGCTGGYTGCTGTGCTCACATATGTGGGCTAT
GGCGTACTCACCCTCTTTGGATATCTTCGAGATTTCTTGAGGCATTGGAGAATTGAAAAGTGCCACCATGCAACAGAAAGGGCGTACTCACCCTCTTTGGATATCTTCGAGATTTCTTGAGGCATTGGAGAATTGAAAAGTGCCACCATGCAACAGAAAG
AGAAGAACAAAAGGACTTTGYGTCCTTGTATCAGGATTTTGAAAACTTCTATACAAGGAACCTCTACATGAGAATCAGAGAGAAGAACAAAAGGACTTTGYGTCCTTGTATCAGGATTTTGAAAACTTCTATACAAGGAACCTCTACATGAGAATCAGAG
ACAACTGGAATCGACCTATCTGTAGTGTGCCTGGAGCCAAGGTGGATATCATGGAGAGAAAATCTCATGACTATAACTGGACAACTGGAATCGACCTATCTGTAGTGTGCCTGGAGCCAAGGTGGATATCATGGAGAGAAAATCTCATGACTATAACTGG
TCATTCAAGTACACAGGGAATATAATTAAAGGTGTAATAAACATGGGTTCCTACAACTATCTTGGATTTGCGAGGAACACTCATTCAAGTACACAGGGAATATAATTAAAGGTGTAATAAACATGGGTTCCTACAACTATCTTGGATTTGCGAGGAACAC
TGGATCATGTCAGGAAGCAGCTGCTGAAGTCCTCÄAGGAGTATGGAGCANGGGTGTGCAGCACTCGTCAGGAAATTGGAATGGATCATGTCAGGAAGCAGCTGCTGAAGTCCTCÄAGGAGTATGGAGCANGGGTGTGCAGCACTCGTCAGGAAATTGGAA
ACCTGGACAAGCATGAAGACCTGGACAAGCATGAAG
Klon 0090-ContigClone 0090 contig
AGCGGATCTTCGGGCCGGGAGGACATTCGGCCTCTGTGAGCCGCAACCTTGCCCAGCGAGCGGTTGGGNGTTCGCCATCTAGCGGATCTTCGGGCCGGGAGGACATTCGGCCTCTGTGAGCCGCAACCTTGCCCAGCGAGCGGTTGGGNGTTCGCCATCT
TAGGAGGATGTTCTCGTCCGTAGCGCACCTGGCGCGGGCGAACCCCTTCAACGCGCCACACCTGCAGCTGGTGCACGATGTAGGAGGATGTTCTCGTCCGTAGCGCACCTGGCGCGGGCGAACCCCTTCAACGCGCCACACCTGCAGCTGGTGCACGATG
GCCTGTCGGGTCCCCGCAGTCCCCCAGCTCCGCCCCGGCGTTCCCGCCACCTGGCCGCCGCCGCCGNGGAAGAGTACAGTGCCTGTCGGGTCCCCGCAGTCCCCCAGCTCCGCCCCGGCGTTCCCGCCACCTGGCCGCCGCCGCCGNGGAAGAGTACAGT
TGNGAATTTGGCTCCATGAAGTATTATGCACTGAGNGGCTTTGGNGGGGTCTTAAGTTGAGGGCTGACACACACTGCTGTTGNGAATTTGGCTCCATGAAGTATTATGCACTGAGNGGCTTTGGNGGGGTCTTAAGTTGAGGGCTGACACACACTGCTGT
TGTTCCCCTGGACTTAGTAAAGYGCCGCATGCAGGTGGACCCTCAGAAGTACAAAGGCATATTTAATGGATTCTCCATTATGTTCCCCTGGACTTAGTAAAGYGCCGCATGCAGGTGGACCCTCAGAAGTACAAAGGCATATTTAATGGATTCTCCATTA
CACTGAAAGAAGATGGCGTTCGNGGKTYGGCTAAAGGATGGGCCCCAACTTTGATTGGCTATTCCATGCAAGGGCTCTGCCACTGAAAGAAGATGGCGTTCGNGGKTYGGCTAAAGGATGGGCCCCAACTTTGATTGGCTATTCCATGCAAGGGCTCTGC
AAATTCGGCTTTTATGAAGTCTTCAAAGCCTTATATAGCAACATACTTGGTGAGGAAAACACCTACCTGTGGCGCACATCAAATTCGGCTTTTATGAAGTCTTCAAAGCCTTATATAGCAACATACTTGGTGAGGAAAACACCTACCTGTGGCGCACATC
ACTGTATTTAGCTTCTTCTGCCAGAGCTGAATTCTTCGCTGACATTGCCCTGGACTCCTATGGAAGCTGCTAAAGTTCGAACTGTATTTAGCTTCTTCTGCCAGAGCTGAATTCTTCGCTGACATTGCCCTGGACTCCTATGGAAGCTGCTAAAGTTCGA
ATTCAAAACCCAGCCTGGKTATGCCAACACCTTGAGGGAAGCTGTTCCCAAAATGTATAAAGAGGAAGGCTTAAATGCGTATTCAAAACCCAGCCTGGKTATGCCAACACCTTGAGGGAAGCTGTTCCCAAAATGTATAAAGAGGAAGGCTTAAATGCGT
TCTACAAGGGCG TGCTCCTCTGTGGATGAGACAGATCCCSTACACCATGATGAAGTTCGCCTGCTTTGAACGTACTGTTTCTACAAGGGCG TGCTCCTCTGTGGATGAGACAGATCCCSTACACCATGATGAAGTTCGCCTGCTTTGAACGTACTGTT
GAAGCTTTGTACAAATTTGTGGTTCCCAAGCCCCGAAGTGAATGTACAAAGGCAGAGCAGCTGGTTGTGACATTTGTGGCGAAGCTTTGTACAAATTTGTGGTTCCCAAGCCCCGAAGTGAATGTACAAAGGCAGAGCAGCTGGTTGTGACATTTGTGGC
AGGTTACATAGCTGGAGTCTTCTGTGCGATCGTCTCCCNCCCTGCTGACTCTGTGGTCTCTGTGCTGAATAAAGAGAAAGAGGTTACATAGCTGGAGTCTTCTGTGCGATCGTCTCCCNCCCTGCTGACTCTGTGGTCTCTGTGCTGAATAAAGAGAAAG
GCAGCTCCGCGTCTCAGGTCCTGCAGAGGCTGGGCTTCAGAGGTGTGTGGAAGGGGCTCTTCGCCCGAATCATCATGATTGCAGCTCCGCGTCTCAGGTCCTGCAGAGGCTGGGCTTCAGAGGTGTGTGGAAGGGGCTCTTCGCCCGAATCATCATGATT
GGCACTCTGACTGCNCTACAGTGGTTCATCTACGACTCTGTGAAGGTCTACTTCAGGCTCCCTCGCCCTCCTCCCCCTGAGGCACTCTGACTGCNCTACAGTGGTTCATCTACGACTCTGTGAAGGTCTACTTCAGGCTCCCTCGCCCTCCTCCCCCTGA
GATGCCAGAGTCTCTGAAGAAGAAGCTTGGGTTAACTGAGTAGATACATCAAAGTAACATGGACTGAATCTGCTTGTTGA TCAGTGTTGAAAGTGCAAAGGAACTTTTATATATTTGACAGTGTAGGAAATGTCTGTTCCTGATATAATTACTGTAGTAC CCTTGCTTAAAGCAAGAGTTTCAAACTTAATGCTAAATAAACCCATCTATTCCTGAAAAAAAAAAAAAAAGATGCCAGAGTCTCTGAAGAAGAAGCTTGGGTTAACTGAGTAGATACATCAAAGTAACATGGACTGAATCTGCTTGTTGA TCAGTGTTGAAAGTGCAAAGGAACTTTTATATATTTGACAGTGTAGGAAATGTCTGTTCCTGATATAATTACTGTAGTAC CCTTGCTTAAAGCAAGAGTTTCAAACTTAATGCTAAATAAACCCATCTATTCCTGAAAAAAAAAAAAAAA
Klon 0091-ContigClone 0091 contig
AGGCACTGCTGGCGACATGGCCGACACGGACCCGGGCTATCCCCGCTCGTCCATCGAGGATGACTTCAACTACGGCAGCT GCGTGGCGTCGGCCAGCGTGCACATCCGCATGGCCTTTCTCAGAAAAGTCTACAGTATCCTCTCTCTGCAAGTCCTCCTG ACTACAGTGACCTCTGCCCTGTTCCTGTATTTCCAAGCTCTGCGGACATTTGTCCATGAAAGCCCTGCCTTAATTG GG GTTWGCTCTGGGATCTCTGGGCTTGATCTTTGCACTGACTCTGCACAGACACACGCATCCTCTGAACCTCTATCTACTCT TTGCATTTACACTGTCAGAATCCCTGGCCG GGCAGCTGTTG TACCTTCTATGATGTATATCTGGTTCTGCAAGCGTTT ATAATGACTACTGCAGTCTTTCTTGGCTTGACTGCCTATACTCTACAATCAAAGAGAGATTTCACCAAATTCGGAGCAGG GTTGWTWGCTGGTWTG GGATTTTGWGCTTGGCAGGATTCTTGAAGCTG TTTTTTACAG GAGACGATGGAGCTGGTCT TGGCCTCTCTAGGCGCCCTCCTCTTCTGTGGGTTCATCATCTATGATACACACTCGCTGATGCACAGACTCTCTCCCGAA GAGTACGTGAWCGCTGCCATCAGTCTCTACATGGATATCATCAACCTCTTCCTGCACCTGKTGAAGTTTCTGGAAGCAGT TAAATAAAΆΆGTAACCGAGCAGTNGTTCAGAGACAGKTCTATTATGAΆAGGACGCTTTGGAATTAAACTTTAAATGGTTA ATAATTAAAAGCCAWATGTGAWCTTTAAAAAAAAAAAAAΆAAAAAAAGGCACTGCTGGCGACATGGCCGACACGGACCCGGGCTATCCCCGCTCGTCCATCGAGGATGACTTCAACTACGGCAGCT GCGTGGCGTCGGCCAGCGTGCACATCCGCATGGCCTTTCTCAGAAAAGTCTACAGTATCCTCTCTCTGCAAGTCCTCCTG ACTACAGTGACCTCTGCCCTGTTCCTGTATTTCCAAGCTCTGCGGACATTTGTCCATGAAAGCCCTGCCTTAATTG GG GTTWGCTCTGGGATCTCTGGGCTTGATCTTTGCACTGACTCTGCACAGACACACGCATCCTCTGAACCTCTATCTACTCT TTGCATTTACACTGTCAGAATCCCTGGCCG GGCAGCTGTTG TACCTTCTATGATGTATATCTGGTTCTGCAAGCGTTT ATAATGACTACTGCAGTCTTTCTTGGCTTGACTGCCTATACTCTACAATCAAAGAGAGATTTCACCAAATTCGGAGCAGG GTTGWTWGCTGGTWTG GGATTTTGWGCTTGGCAGGATTCTTGAAGCTG TTTTTTACAG GAGACGATGGAGCTGGTCT TGGCCTCTCTAGGCGCCCTCCTCTTCTGTGGGTTCATCATCTATGATACACACTCGCTGATGCACAGACTCTCTCCCGAA GAGTACGTGAWCGCTGCCATCAGTCTCTACATGGATATCATCAACCTCTTCCTGCACCTGKTGAAGTTTCTGGAAGCAGT TAAATAAAΆΆGTAACCGAGCAGTNGTTCAGAGACAGKTCTATTATGAΆAGGACGCTTTGGAATTAAACTTTAAATGGTTA ATAATTAAAAGCCAWATGTGAWCTTTAAAAAAAAAAAAAΆAAAAAA
Klon 0094-ContigClone 0094 contig
AGGGCCGCAGCAGGCGCGAGCGCCCAGGACCTGCCAGCTGAGCCTTCCGCCGYCGCCATGGGACAGAACGACCTGATGGG CACGGCCGAGGACTTCGCCGACCAGTTCCTTCGAGTCACCAAGCAGTACCTGCCTCATGTGGCGCGCCTCTGCCTGATCA GCACCTTCCTGGAGGATGGCATCCGCATGTGGTTCCAGWGGAGTGAGCAGYGTGACTATATCGACACCACCTGGAGCTGT GGCTACCTGTTGGCCTCATCCTTCGTGTTCCTCAACCTGCTGGGACAATTGACTGGCTGTGTTTTGGTGCTGAGCAGGAA CTTCGTGCAGTATGCCTGCTTTGGGCTGTTTGGAATCATCGCACTGCAGACAATTGCCTATAGCATTTTGTGGGACTTGA AGTTTCTCATGAGGAACCTGGCTTTAGGAGGAGGTTTGCTGCTGCTCTTGGCAGAATCCCGCTCAGAAGGGAAGAGCATG TTTGCTGGTGTCCCAACCATGCGTGAGAGCTCCCCCAAGCAGTACATGCAGCTTGGAGGΆAGGGTCCTGCTGGTCCTGAT GTTCATGACCCTCCTTCACTTTGATGCCAGCTTCTTTTCTATCATCCΆGAACATTGTGGGCACAGCTCTGATGATCTTAG TAGCCATCGGTTTTAAAACCAAGCTGGCAGCTTTGACΤCTCGTCGTCTGGCTGTTTGCCATCAACGTGTATTTCAACGCC TTCTGGACAATCCCGGTATATAAACCCATGCATGACTΤCCTGAAATACGACTTCTTCCAGACCATGTCAGTGATTGGAGG CCTGCTCCTGRTGGTGGCTCTTGG CCAGGGGGTGTGTCCATGGATGAGAΆGAARΆΆAGAGTGGTAACACAC GATCCCT CCCCTCCCTGGCTGAGGCRCAGGGCCCTGGCCTGGTTCAGGGCÄRAGTCAACAAACTGCCGGCGTTTGTGTGTCCTTCTC CCTTCCCCTCCCTTGGTAAAGGCACAGATGTTTTGAGAACTTTATTTGCAGACACCTGAAAATTGGAGATAAAATCTTTG GAAAAAAAAΆAAAAAΆAAAGGGCCGCAGCAGGCGCGAGCGCCCAGGACCTGCCAGCTGAGCCTTCCGCCGYCGCCATGGGACAGAACGACCTGATGGG CACGGCCGAGGACTTCGCCGACCAGTTCCTTCGAGTCACCAAGCAGTACCTGCCTCATGTGGCGCGCCTCTGCCTGATCA GCACCTTCCTGGAGGATGGCATCCGCATGTGGTTCCAGWGGAGTGAGCAGYGTGACTATATCGACACCACCTGGAGCTGT GGCTACCTGTTGGCCTCATCCTTCGTGTTCCTCAACCTGCTGGGACAATTGACTGGCTGTGTTTTGGTGCTGAGCAGGAA CTTCGTGCAGTATGCCTGCTTTGGGCTGTTTGGAATCATCGCACTGCAGACAATTGCCTATAGCATTTTGTGGGACTTGA AGTTTCTCATGAGGAACCTGGCTTTAGGAGGAGGTTTGCTGCTGCTCTTGGCAGAATCCCGCTCAGAAGGGAAGAGCATG TTTGCTGGTGTCCCAACCATGCGTGAGAGCTCCCCCAAGCAGTACATGCAGCTTGGAGGΆAGGGTCCTGCTGGTCCTGAT GTTCATGACCCTCCTTCACTTTGATGCCAGCTTCTTTTCTATCATCCΆGAACATTGTGGGCACAGCTCTGATGATCTTAG TAGCCATCGGTTTTAAAACCAAGCTGGCAGCTTTGACΤCTCGTCGTCTGGCTGTTTGCCATCAACGTGTATTTCAACGCC TTCTGGACAATCCCGGTATATAAACCCATGCATGACTΤCCTGAAATACGACTTCTTCCAGACCATGTCAGTGATTGGAGG CCTGCTCCTGRTGGTGGCTCTTGG CCAGGGGGTGTGTCCATGGATGAGAΆGAARΆΆAGAGTGGTAACACAC GATCCCT CCCCTCCCTGGCTGAGGCRCAGGGCCCTGGCCTGGTTCAGGGCÄRAGTCAACAAACTGCCGGCGTTTGTGTGTCCTTCTC CCTTCCCCTCCCTTGGTAAA GGCACAGATGTTTTGAGAACTTTATTTGCAGACACCTGAAAATTGGAGATAAAATCTTTG GAAAAAAAAΆAAAAAΆAA
Klon 0095-ContigClone 0095 contig
AGCTTCTTCAGTCGGGACGGGGTCAGCGAGCGGCTGCTTCGTGGAGCAGAGAGGTGCATCACCAGGTTCCCGATGAACCC AGAGAACCCTCCACCGTAΓCCGGGCCCCGGGCCAACAGCCCCATACCCACCTTATCCACAACAGCCAAWGGGGCCAATGG GGCCTATGGGAGCCCCACCTCCTCAGGGGTACCCCTACCCACCACCTCAGGGGTACCCCTATCAAGGATACCCACAGTAC GGCTGGCAGGGTGGACCTCAGGAGCCTCCTAAGACCACAGTGTATGTGGTGGAAGACCAAAGAAGAGACGACCTGGGCCC ATCCACCTGCCTCACAGCCTGCTGGACTGCTCTGTGTTGCTGCTGCCTCTGGGACATGCTCACCTGATCACCTGATGAGC CCAGCTCTTCCGCTTGGCCGCTCTGTGCCACCTCCGATAΆGTGTGCCTGGCCCCATCTCTTCTGATTGCTATAAAGTGGC TAGCTCTGCGCAGACACCTCTACTTTCTGTCCTATGGAGTTTTAGATTAGTGAGGGTTACTGCTATTTAGTTCAGTGACT TGATCTTTTTAATGTTCAAAACTCATTTCTTACTGATCTTTAAAAAATGTGCTAAATAATTTACTTTTTTGGCCAAAGGC TTAGTTATGAAATATATATTTATAATGTTTAAATTATACACTCAAGGTAATGGCAATATGTGACACACTACTAGATCTCT CTGCTCATACCCTTTATGTTTCAATAAATCTGTCCAAAATCAAAAAAAAAAAAAAAAAAAAAGCTTCTTCAGTCGGGACGGGGTCAGCGAGCGGCTGCTTCGTGGAGCAGAGAGGTGCATCACCAGGTTCCCGATGAACCC AGAGAACCCTCCACCGTAΓCCGGGCCCCGGGCCAACAGCCCCATACCCACCTTATCCACAACAGCCAAWGGGGCCAATGG GGCCTATGGGAGCCCCACCTCCTCAGGGGTACCCCTACCCACCACCTCAGGGGTACCCCTATCAAGGATACCCACAGTAC GGCTGGCAGGGTGGACCTCAGGAGCCTCCTAAGACCACAGTGTATGTGGTGGAAGACCAAAGAAGAGACGACCTGGGCCC ATCCACCTGCCTCACAGCCTGCTGGACTGCTCTGTGTTGCTGCTGCCTCTGGGACATGCTCACCTGATCACCTGATGAGC CCAGCTCTTCCGCTTGGCCGCTCTGTGCCACCTCCGATAΆGTGTGCCTGGCCCCATCTCTTCTGATTGCTATAAAGTGGC TAGCTCTGCGCAGACACCTCTACTTTCTGTCCTATGGAGTTTTAGATTAGTGAGGGTTACTGCTATTTAGTTCAGTGACT TGATCTTTTTAATGTTCAAAACTCATTTCTTACTGATCTTTAAAAAATGTGCTAAATAATTTACTTTTTTGGCCAAAGGC TTAGTTATGAAATATATATTTATAATGTTTAAATTATACACTCAAGGTAATGGCAATATGTGACACACTACTAGATCTCT CTGCTCATACCCTTTATGTTTCAATAAATCTGTCCAAAATCAAAAAAAAAAAAAAAAAAAA
Klon 0097-T7-SequenzClone 0097-T7 sequence
GTTTCCAACAAGGACGACATGAAGACCTCACTCAAGAAAGTTGTGAAGGAGACATCGTATGAGATGATGATGCAGTGTGTGTTTCCAACAAGGACGACATGAAGACCTCACTCAAGAAAGTTGTGAAGGAGACATCGTATGAGATGATGATGCAGTGTGT
ATCGCGAATGCTGGCCCATCCCTTACACGTGATCTCGATGCGATGCATGGTGCAGTTTGTGGGACGGGAGGCCAAGTACAATCGCGAATGCTGGCCCATCCCTTACACGTGATCTCGATGCGATGCATGGTGCAGTTTGTGGGACGGGAGGCCAAGTACA
G GGTGTGCTGAGTTCTATTGGGAAGATCTTCAAGGAAGAGGGGCTGCTGGGATTCTTCGTTGGCτTAATCCCTCACCTCG GGTGTGCTGAGTTCTATTGGGAAGATCTTCAAGGAAGAGGGGCTGCTGGGATTCTTCGTTGGCτTAATCCCTCACCTC
CTGGGCGATG GGTTTTCTTGTGGGGCTGTAACCTGCTGGCCCACTTCATCAATGCCTACTTGGTGGACGACAGCGTGAGCTGGGCGATG GGTTTTCTTGTGGGGCTGTAACCTGCTGGCCCACTTCATCAATGCCTACTTGGTGGACGACAGCGTGAG
TGACACCCCAGGGGGGCTGGGAAACGACCAGAATCCAGGTTCCCAGTTTAGCCAGGCCCTGGCCATCCGGAGCTACACCATGACACCCCAGGGGGGCTGGGAAACGACCAGAATCCAGGTTCCCAGTTTAGCCAGGCCCTGGCCATCCGGAGCTACACCA
AGTTTGYGATGGGGATTGCAGYGAGCATGCTGACCTACCCCCTTYCTGCTCGCTGGAGATCTCATGGCAGTGAACAACTGAGTTTGYGATGGGGATTGCAGYGAGCATGCTGACCTACCCCCTTYCTGCTCGCTGGAGATCTCATGGCAGTGAACAACTG
TGGGCTGCGGGCTGGACTCCCTCCGTATTCCCCTGTGTTCAAGTCCTGGATCCACTGCTGGAAGTACCTGAGTGTGCAGGTGGGCTGCGGGCTGGACTCCCTCCGTATTCCCCTGTGTTCAAGTCCTGGATCCACTGCTGGAAGTACCTGAGTGTGCAGG
GCCAGCTCTTCCGCGGCTCCAGCCTGCTTTTCCGCCGGGTGTCATCGGGGTCATGCTTTGCCCTGGAGTAACCTAAGCTGGCCAGCTCTTCCGCGGCTCCAGCCTGCTTTTCCGCCGGGTGTCATCGGGGTCATGCTTTGCCCTGGAGTAACCTAAGCTG
CCCGACCAAACATTTATGGGGTCTTAGCCTACCCCTGGTGAGGACCCATCATCTCAGATGCCCCÄAGGGTGACTCCAGCCCCCGACCAAACATTTATGGGGTCTTAGCCTACCCCTGGTGAGGACCCATCATCTCAGATGCCCCÄAGGGTGACTCCAGCC
CAGCCCTGGCTTCATGTCCATATTTGCCATGTGTCTGTCCAGATGTCAGCCCTGGCTTCATGTCCATATTTGCCATGTGTCTGTCCAGATGT
Klon 0097-BGH-Sequenz CTTTTTTTTTTTTTTTTTTTCACCACCAATACATTTATTCGAGGAGATGGGTCTATCTTACCACGAGGGGAGGACTAGAT GTCGGYCTATGTAACCTGTGCGTATTCGCACCCAGCACAGTGACTGAACCCTCACACCTGGCGTCACCAGCACAGACAAG CAGATGAGGGGATGGTCTGAGGAGAACATGATTTCCTATTCAGGAGAAGGCACCACCCTTGTATAAGAAAATTAGTGTTGClone 0097 BGH sequence CTTTTTTTTTTTTTTTTTTTCACCACCAATACATTTATTCGAGGAGATGGGTCTATCTTACCACGAGGGGAGGACTAGAT GTCGGYCTATGTAACCTGTGCGTATTCGCACCCAGCACAGTGACTGAACCCTCACACCTGGCGTCACCAGCACAGACAAG CAGATGAGGGGATGGTCTGAGGAGAACATGATTTCCTATTCAGGAGAAGGCACCACCCTTGTATAAGAAAATTAGTGTTG
GGAACATAGCGCCAGCCTCCCATGGCCCAGGTGTGATGGCGCCCAATTTACAAAGCAGGAAGTGGGGGGCGGSGGTGCTTGGAACATAGCGCCAGCCTCCCATGGCCCAGGTGTGATGGCGCCCAATTTACAAAGCAGGAAGTGGGGGGCGGSGGTGCTT
CTGGCTGACTGGCAGGATGAGCTGGGCTAGAGGTGCAGGGAÄGCCTTGCCACΓGAGTGACGTTTGCCTCTGCAGCCTGCC TCTGCCΓGAGTACAAGATGGACTCCAGTACCTCTAGGCAGGAAGGGGATGCCACCCCACCACTGCTCCTGGCTGACTGGCAGGATGAGCTGGGCTAGAGGTGCAGGGAÄGCCTTGCCACΓGAGTGACGTTTGCCTCTGCAGCCTGCC TCTGCCΓGAGTACAAGATGGACTCCAGTACCTCTAGGCAGGAAGGGGATGCCACCCCACCACT
Klon 0098-T7-SequenzClone 0098-T7 sequence
AGGGCGGAGGAAGCGGACTGTTCCGGAGCTCTGCCTAGCCGGGCCCAACCTTTGCTCCAGAGATCATGGCTGCCGAGGAT GTGGTGGCGACTGGCGCCGACCCTGAGCGAGCTAGAGGGCGGCGGGCTGCTGCACGAGATTTTCACGTCCTCCTCTCAAC CCTGCTCCTCCTGGGCCTCTGCATCTTCCTGCTCTACAAGATCGTTCCGCGGGGACCAGCCCGGTGCYAGTGGCGACAAC GACGAYGACGAACCACCCCCGCTGCCCCGCCTCAAGCGGCGCGACTTCACCCCTGCCGAGCTGAGGCGTTTCGATGGCGT CCAGGACCCGCGCATTCTCATGGCCATCAACGGYAAGGTGΓTCGACGTGACCΆAAGGCCGCAAGTTCTACGGGCCTGAGG GGCCATATGGGGTCTTTGCCGGAAGAGATGCATCCAGGGGCCTTGCCACATTTTGCCTGGACAAAGAAGCACTGAAGGAT GAGTATGACGACCTTTCTGACCTCACCCCTGCACAGCAGGAGACCCTGAGTGACTGGGACTCTCAGTTCACTTTCAAGTA TCATCACGTGGGAAAACTGCTGAAGGAAGGGGAGGAGCCTACTGTGTACTCAGATGATGAAGAACCAAAAGAYGAGACAG CTCGGAAGAATGAATGAAGCATTCGGTGGAGCATATCTATTTTTGΓATTTTGCAAAATCATTTGTAACATTCCAGTCTGT CTTTACAACATGGTGATTTCAATATTTAGAAAAGNTTTGAACTTGCTGTAAATTTTTTAAGTAACATCACTAGTGACACC GATAAAATCAACTCGAGGGCGGAGGAAGCGGACTGTTCCGGAGCTCTGCCTAGCCGGGCCCAACCTTTGCTCCAGAGATCATGGCTGCCGAGGAT GTGGTGGCGACTGGCGCCGACCCTGAGCGAGCTAGAGGGCGGCGGGCTGCTGCACGAGATTTTCACGTCCTCCTCTCAAC CCTGCTCCTCCTGGGCCTCTGCATCTTCCTGCTCTACAAGATCGTTCCGCGGGGACCAGCCCGGTGCYAGTGGCGACAAC GACGAYGACGAACCACCCCCGCTGCCCCGCCTCAAGCGGCGCGACTTCACCCCTGCCGAGCTGAGGCGTTTCGATGGCGT CCAGGACCCGCGCATTCTCATGGCCATCAACGGYAAGGTGΓTCGACGTGACCΆAAGGCCGCAAGTTCTACGGGCCTGAGG GGCCATATGGGGTCTTTGCCGGAAGAGATGCATCCAGGGGCCTTGCCACATTTTGCCTGGACAAAGAAGCACTGAAGGAT GAGTATGACGACCTTTCTGACCTCACCCCTGCACAGCAGGAGACCCTGAGTGACTGGGACTCTCAGTTCACTTTCAAGTA TCATCACGTGGGAAAACTGCTGAAGGAAGGGGAGGAGCCTACTGTGTACTCAGATGATGAAGAACCAAAAGAYGAGACAG CTCGGAAGAATGAATGAAGCATTCGGTGGAGCATATCTATTTTTGΓATTTTGCAAAATCATTTGTAACATTCCAGTCTGT CTTTACAACATGGTGATTTCAATATTTAGAAAAGNTTTGAACTTGCTGTAAATTTTTTAAGTAACATCACTAGTGACACC GATAAAATCAACTCG
Klon 0099-ContιgClone 0099-Contιg
AGCTCGAAÄGCGACATGGCGGTTCTCTTAAAGCTGGGCGTTCTCTGCAGTGGCCAAGGAGCTCGAGCTCTCCTACTCCGAAGCTCGAAÄGCGACATGGCGGTTCTCTTAAAGCTGGGCGTTCTCTGCAGTGGCCAAGGAGCTCGAGCTCTCCTACTCCGA
AGCCGGGTGGTCAGACCCGCTTATGTGTCAGCATTTCTCCAGGACCAGCCTACCCAAGGACGGTGTGGTACCCAGCACATAGCCGGGTGGTCAGACCCGCTTATGTGTCAGCATTTCTCCAGGACCAGCCTACCCAAGGACGGTGTGGTACCCAGCACAT
TCACCTGTCACCAAGCCACCACTCTGGTTCCAAGGCTGCATCTCTCCACTGGACCAGTGAGAGGGTTGTCAGTGTTCTGCTCACCTGTCACCAAGCCACCACTCTGGTTCCAAGGCTGCATCTCTCCACTGGACCAGTGAGAGGGTTGTCAGTGTTCTGC
TCTTGGGGCTGATCCCTGCTGGGTACTTGAATCCCTGCTCTGTGGTGGACTACTCTCTGGCTGCAGCCCTCACCCTGCACTCTTGGGGCTGATCCCTGCTGGGTACTTGAATCCCTGCTCTGTGGTGGACTACTCTCTGGCTGCAGCCCTCACCCTGCAC
AGTCACTGGGGCCTTGGACAAGTGGTTACCGACTACGTTCATGGGGACACCCTGCCGAAGGCTGCCAGGGCAGGCCTCTT GGCACTCTCAGCTTTGACCTTTGCTGGGCTTTGCTACTTCAATTACCACGATGTCGGCÄTCTGCAGAGCGGTTGCCATGC TGTGGAAGCTCTGACCTGGGTGCAGCACTTTGATTGTGTGCCTCCTTGCCTCTGCTΓTACCAATGCCGTTCACCTCGCAG TGAGGGGGGATGAAGGATAAGCCCAΓTGGTGGGCAGAΆTGTCTTCTAATTACATGGTTATTTTCAGAATTTAΓTTGTTGA GGAAGAGGTTΓGAGGAGTTAGGTTCGACCATTCGTGAGTCTGTGTTCCATACTCCACTGAGTGTGGGCACTAGCTCACAG CCTCGCGGTGAGACTGAACATTTCATGAGCTCATGKTGCCTΓTGACCACCATTTTCTTAAGGAGAGCCAGCTGATTGCTG TCAGGATAAGAGCATCTCTTCAGCCAGGAGGGAGGCCTGTΓCCCTCCTGAGTTAGACTTTGCATGAAGCTCGAAAGTATT CCCTTTGGAACCTCCCATTCTTGTTCΆGGTGACACCAGCTCTGTTGATGGCTCTGCΓTCTAGGGAACATTTAATCAGGAG ATGCTCΓCAATGACTAATTTGTCTAAGTCTTAGGAAGGAGGTTGAGGAAAGCTGGATTTAGACAAGTTCAATTTAGGGAG TTCTCCTTGTTTGTGGATTAAAÄTATGACAGATTGCΆAACAGACTACTCTTCAAATGTATCTCAATTGTGCAGAAGTGAG CTGTCCAAAAGTATAAGACTAAGTGATAAACTGTCTTCCCACCGTGGGAGTTGTTAATGAGAAAGAAAGTGTACTCTGAA AAAACAAAATTTTTAAATAAAATATΤATATAATGAAAAAAAAAAAAAAAAAAAGTCACTGGGGCCTTGGACAAGTGGTTACCGACTACGTTCATGGGGACACCCTGCCGAAGGCTGCCAGGGCAGGCCTCTT GGCACTCTCAGCTTTGACCTTTGCTGGGCTTTGCTACTTCAATTACCACGATGTCGGCÄTCTGCAGAGCGGTTGCCATGC TGTGGAAGCTCTGACCTGGGTGCAGCACTTTGATTGTGTGCCTCCTTGCCTCTGCTΓTACCAATGCCGTTCACCTCGCAG TGAGGGGGGATGAAGGATAAGCCCAΓTGGTGGGCAGAΆTGTCTTCTAATTACATGGTTATTTTCAGAATTTAΓTTGTTGA GGAAGAGGTTΓGAGGAGTTAGGTTCGACCATTCGTGAGTCTGTGTTCCATACTCCACTGAGTGTGGGCACTAGCTCACAG CCTCGCGGTGAGACTGAACATTTCATGAGCTCATGKTGCCTΓTGACCACCATTTTCTTAAGGAGAGCCAGCTGATTGCTG TCAGGATAAGAGCATCTCTTCAGCCAGGAGGGAGGCCTGTΓCCCTCCTGAGTTAGACTTTGCATGAAGCTCGAAAGTATT CCCTTTGGAACCTCCCATTCTTGTTCΆGGTGACACCAGCTCTGTTGATGGCTCTGCΓTCTAGGGAACATTTAATCAGGAG ATGCTCΓCAATGACTAATTTGTCTAAGTCTTAGGAAGGAGGTTGAGGAAAGCTGGATTTAGACAAGTTCAATTTAGGGAG TTCTCCTTGTTTGTGGATTAAAÄTATGACAGATTGCΆAACAGACTACTCTTCAAATGTATCTCAATTGTGCAGAAGTGAG CTGTCCAAAAGTATAAGACTAAGTGATAAACTGTCTTCCCACCGTGGGAGTTGTTAATGAGAAAGAAAGTGTACTCTGAA AAAACAAAATTTTTAAATAAAATATΤATATAATGAAAAAAAAAAAAAAAAAA
Klon 0100-ContigClone 0100 contig
AGCTAGGATCTTTAGTCTTCAACTCCTACTGCTCCTTCTAACCCAGCAGCCCCGGATAATGCAGCCCAGGAGGAGCTCAT GATCACCCTGATCACAGGATTGGCGΓCCCTCACGTCGAGAACCTCCATGGGCATCAΓCGKTGKTGGGGGCCGGTRATTTG GAAAACAGGTGGGCTGGAAACTAATCTCTGTCACCTΓAAGTATGTACGGAGCTCTGTΆCCTTTATGAGAGGCTGACGTGG ACGACCCGTGCGAAAGAGAGAGCGTTTAAGCAGCAGTTTGTAAACTATGCAACTGAGAAGCTGCAGATGATTGTGAGCTT CACCAGTGCAAÄCTGCAGCCACCAAGTACAGCAAGAAATGGCCACTACTTTTGCTCGACTGTGCCAACAAGTCTGATGTT ACTCAGAAACATCTGGAAGAGGAAAΓTGCAAGATTATCCAAAGAGATAGACCAACTGGAGAAAATACAGAACAACTCAAA GCTCTTAAGAAATAAAGCTATTCAACTCTGAAAGTGAGCTGGAGAATTTTTSGAAGCAGTTTCTACACCCGAGCAGTGGA GAATCCTAACGGCAGAGGCACTGTAGGAGGAAGCGGACTTGGAAGATGGGAAATGTTACTTTATGAAATGACCTCAGTAC AAATTACTAACTCTTAGTATCGATGCCTTGCGGAGATTGTGGTAATGACCTGTCTCAGGGGTTGCACCTTTGGAAGTGTT GTGATTCGCCTTGTCTTAGCATTAGTTTGGAGTAAAGACTGAATTGTTAAGGTTAAATGATGAATTCCTTTAGAAACAGT GGAACCGGCTGTKCGGCCCCTGAGGGTGGGTCCTGCAGCTCCTCACCAGGCTGGCTGTCTGCGGCTCTCAGAAGCTGCTT CCTGGCATCCAGGAGTTAGAGACCTTTTCATCCTTTCTCAGTGCTAGTTCTTGATGCTTCTTTAATGGGAATAGTGAACT TGTTTATAASCCGATTTGCTCAAACGAGGGGTGTGGGCTGCTCCTGGGGGGGTCCTGCAATCACTCTGTCCTCACAGCAA GGATGTAACCMCTACTAAACAGTTTTTACTTTCTTTTATTCCCATTAAAGCTGATGTGAAATAGTAAAAAAAAAAAAAAA AAAAAAAAAGCTAGGATCTTTAGTCTTCAACTCCTACTGCTCCTTCTAACCCAGCAGCCCCGGATAATGCAGCCCAGGAGGAGCTCAT GATCACCCTGATCACAGGATTGGCGΓCCCTCACGTCGAGAACCTCCATGGGCATCAΓCGKTGKTGGGGGCCGGTRATTTG GAAAACAGGTGGGCTGGAAACTAATCTCTGTCACCTΓAAGTATGTACGGAGCTCTGTΆCCTTTATGAGAGGCTGACGTGG ACGACCCGTGCGAAAGAGAGAGCGTTTAAGCAGCAGTTTGTAAACTATGCAACTGAGAAGCTGCAGATGATTGTGAGCTT CACCAGTGCAAÄCTGCAGCCACCAAGTACAGCAAGAAATGGCCACTACTTTTGCTCGACTGTGCCAACAAGTCTGATGTT ACTCAGAAACATCTGGAAGAGGAAAΓTGCAAGATTATCCAAAGAGATAGACCAACTGGAGAAAATACAGAACAACTCAAA GCTCTTAAGAAATAAAGCTATTCAACTCTGAAAGTGAGCTGGAGAATTTTTSGAAGCAGTTTCTACACCCGAGCAGTGGA GAATCCTAACGGCAGAGGCACTGTAGGAGGAAGCGGACTTGGAAGATGGGAAATGTTACTTTATGAAATGACCTCAGTAC AAATTACTAACTCTTAGTATCGATGCCTTGCGGAGATTGTGGTAATGACCTGTCTCAGGGGTTGCACCTTTGGAAGTGTT GTGATTCGCCTTGTCTTAGCATTAGTTTGGAGTAAAGACTGAATTGTTAAGGTTAAATGATGAATTCCTTTAGAAACAGT GGAACCGGCTGTKCGGCCCCTGAGGGTGGGTCCTGCAGCTCCTCACCAGGCTGGCTGTCTGCGGCTCTCAGAAGCTGCTT CCTGGCATCCAGGAGTTAGAGACCTTTTCATCCTTTCTCAGTGCTAGTTCTTGATGCTTCTTTAATGGGAATAGTGAACT TGTTTATAASCCGATTTGCTCA AACGAGGGGTGTGGGCTGCTCCTGGGGGGGTCCTGCAATCACTCTGTCCTCACAGCAA GGATGTAACCMCTACTAAACAGTTTTTACTTTCTTTTATTCCCATTAAAGCTGATGTGAAATAGTAAAAAAAAAAAAAAA AAAAAAAA
Klon 0101-T7-SequenzClone 0101-T7 sequence
AGTCTGCGCCCCTGTACCCTAGGTCΓAGTAGTGAACCCGCACCCGRKCAAAGAAGTCTAGGCCGGRGTCCGCAGMATCAG CCATCACTTCGCACAAAGCAGCGCAGCTCCGGGACCGCCGAGGACCACAGCGGCGCGGCAGCGGYGCGGMGACACTCAGT GCACCGTATGCCCCTGCGMCCCTGCCGAGGCAACCGGAGCGCCCCGAGAGACCGCGCCGCCGCGGGGWCCAGGTGCAGYT AGCGAGCCTAGCCCGCAGCGCGCAGTCGCGGGAGAGCGGGGAGCGGCAAGCAACAGGGAGCGGGACGGCGGCGAGGCGCT CGCGGGCCCCTCCTGCTGCCCGCGCCCGGCGAGCTCATGGCGGCCATCCGCAAGAAGCTGGNGGNGGNGGGCGACGGCGC GTGCGGCAAGACGTGCCTGCTGATCGTGTTCAGTAAAGACGAATTCCCCGAGGTGTACGTGCCCACCGTGTTCGAGAACT ATGTGGCGGACATCGAGGTGGΆCGGCAAGCAGGTGGAGCTGGCGCTGNGGGACACGGCAGGCCAGGAGGACTACGATCGT TTACGGCCGCTCTCCTATCCGGACACCGACGTCATCCTTATGTGCTTCTCGGTGGACAGCCCGGACTCTCTCGAGAACAT CCCCGAGAAGTGGGTGCCCGAGGTAAAGCACTTCTGCCCCAATGTGCCCATCATCCTGGTGGCCAACAAAAAAGACCTGN TCAGCGACNAGCATGTCCGACGGAGCTGGCCCGCATGAAGCAGGAGCCAGTNCNGCACGGATTGACGGAGTCTGCGCCCCTGTACCCTAGGTCΓAGTAGTGAACCCGCACCCGRKCAAAGAAGTCTA GG CCGGRGTCCGCAGMATCAG CCATCACTTCGCACAAAGCAGCGCAGCTCCGGGACCGCCGAGGACCACAGCGGCGCGGCAGCGGYGCGGMGACACTCAGT GCACCGTATGCCCCTGCGMCCCTGCCGAGGCAACCGGAGCGCCCCGAGAGACCGCGCCGCCGCGGGGWCCAGGTGCAGYT AGCGAGCCTAGCCCGCAGCGCGCAGTCGCGGGAGAGCGGGGAGCGGCAAGCAACAGGGAGCGGGACGGCGGCGAGGCGCT CGCGGGCCCCTCCTGCTGCCCGCGCCCGGCGAGCTCATGGCGGCCATCCGCAAGAAGCTGGNGGNGGNGGGCGACGGCGC GTGCGGCAAGACGTGCCTGCTGATCGTGTTCAGTAAAGACGAATTCCCCGAGGTGTACGTGCCCACCGTGTTCGAGAACT ATGTGGCGGACATCGAGGTGGΆCGGCAAGCAGGTGGAGCTGGCGCTGNGGGACACGGCAGGCCAGGAGGACTACGATCGT TTACGGCCGCTCTCCTATCCGGACACCGACGTCATCCTTATGTGCTTCTCGGTGGACAGCCCGGACTCTCTCGAGAACAT CCCCGAGAAGTGGGTGCCCGAGGTAAAGCACTTCTGCCCCAATGTGCCCATCATCCTGGTGGCCAACAAAAAAGACCTGN TCAGCGACNAGCATGTCCGACGGAGCTGGCCCGCATGAAGCAGGAGCCAGTNCNGCACGGATTGACGG
Klon 0102-ContigClone 0102 contig
AGGTTTCCTCGCCGCCGGCCAAGATGAACCGATTCTTCGGAAAAGCGAAACCCAAGGCTCCGCCACCTAGCTTGACGGAC TGCATTGGGACGGTGGATAGCAGGGCAGAATCCATTGACAAAAAGATTTCCCGGCTGGATGCTGAACTAGTGAAATATAA GGATCAAATCAAGAΆGATGAGAGAGGGTCCTGCTAAGAACATGGTCAAACAGAAAGCCCTGAGΆGTTTTAAAGCAAAAGC GGATGTATGAGCAACAGCGAGACAACCTGGCCCAACAGTCCTTTAACATGGAGCAAGCTAATTACACCATCCAGTCACTA AAGGACACCAAGACCACGGTTGATGCCATGAAGTTGGGAGTAAAGGAAATGAAGAAGGCATATAAGGAAGTAAAAATTGA CCAGATTGAGGACTTACAAGACCAGCTGGAGGATATGATGGAAGATGCAAATGAGATCCAGGAAGCCCTGGGCCGCAGCT ACGGCACCCCAGAGTTAGATGAGGACGACCTGGAAGCAGAGTTAGATGCGCTGGGCGATGAGCTTCTGGCTGATGAAGAT AGCTCCTACTTGGATGAGGCAGCTTCCGCTCCTGCAATTCCGGAAGGTGTTCCCACTGACACAAAAAACAAGGATGGCCG TGCTGNNGGATGAATTTGNACTGCCGCAGATTCCCGCTTCGTAGACTTACAACATTCAGCACGTGATGTGAAACAACAAG AGAAGTATTCTGGGACTAGGAAATAGTTCCCCGATCTGCCAACCAGATTTAGGTTTCTTTCCTTTCTTTGAWGAAAAAGC TNTNTACACTGCTCCNGNATTTTTATTTTTTCCGNTAAGGAATTCATTGCTTTΤGGGAAACTGTCTTTACTAAAACTTGA TTCNTTTTTTTTTTTTTCTCTTAGGAAAGACTAATTGAAAAGTACCCTTGACTTTGTATGACTTGTTTTCATTCATTAAC AATAATCTGAAATTAAACCAAGGAGATGAGACTCTGAATTCTATGGTAGNGTAAGTACAGTCTCAGTGNGCTGATACATT GATAAGATAAAAGTGATTGATGAGATTGGGACTGCTGATAGTATGCTTCAGAACCCTTGTCTGTTGTGGTATTGTANATG GGTTTAAGTCATGGCCTCTTTTGATANATTTTGTTGTGTCATGTGAGCAAGTCATTNCACGATNTACTGTTGGAATGAAC TGTCTCTTCGGTATCATGAGTTACTATTTTGATTCCATGGTTCCCTCCAGTATACTAGCCTGRCTTGTAATGAATAATGA ATATTTCTTGATATTTÄATGKATAGGRCMTTTATTTATACTCAAT AATATTTTTCMAAAGGAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAGGTTTCCTCGCCGCCGGCCAAGATGAACCGATTCTTCGGAAAAGCGAAACCCAAGGCTCCGCCACCTAGCTTGACGGAC TGCATTGGGACGGTGGATAGCAGGGCAGAATCCATTGACAAAAAGATTTCCCGGCTGGATGCTGAACTAGTGAAATATAA GGATCAAATCAAGAΆGATGAGAGAGGGTCCTGCTAAGAACATGGTCAAACAGAAAGCCCTGAGΆGTTTTAAAGCAAAAGC GGATGTATGAGCAACAGCGAGACAACCTGGCCCAACAGTCCTTTAACATGGAGCAAGCTAATTACACCATCCAGTCACTA AAGGACACCAAGACCACGGTTGATGCCATGAAGTTGGGAGTAAAGGAAATGAAGAAGGCATATAAGGAAGTAAAAATTGA CCAGATTGAGGACTTACAAGACCAGCTGGAGGATATGATGGAAGATGCAAATGAGATCCAGGAAGCCCTGGGCCGCAGCT ACGGCACCCCAGAGTTAGATGAGGACGACCTGGAAGCAGAGTTAGATGCGCTGGGCGATGAGCTTCTGGCTGATGAAGAT AGCTCCTACTTGGATGAGGCAGCTTCCGCTCCTGCAATTCCGGAAGGTGTTCCCACTGACACAAAAAACAAGGATGGCCG TGCTGNNGGATGAATTTGNACTGCCGCAGATTCCCGCTTCGTAGACTTACAACATTCAGCACGTGATGTGAAACAACAAG AGAAGTATTCTGGGACTAGGAAATAGTTCCCCGATCTGCCAACCAGATTTAGGTTTCTTTCCTTTCTTTGAWGAAAAAGC TNTNTACACTGCTCCNGNATTTTTATTTTTTCCGNTAAGGAATTCATTGCTTTΤGGGAAACTGTCTTTACTAAAACTTGA TTCNTTTTTTTTTTTTTCTCTTAGGAAAGACTAATTGAAAAGTACCCTTGACTTTGTATGACTTGTTTTCATTCATTAAC AATAATCTGAAATTAAACCAAGGAG ATGAGACTCTGAATTCTATGGTAGNGTAAGTACAGTCTCAGTGNGCTGATACATT GATAAGATAAAAGTGATTGATGAGATTGGGACTGCTGATAGTATGCTTCAGAACCCTTGTCTGTTGTGGTATTGTANATG GGTTTAAGTCATGGCCTCTTTTGATANATTTTGTTGTGTCATGTGAGCAAGTCATTNCACGATNTACTGTTGGAATGAAC TGTCTCTTCGGTATCATGAGTTACTATTTTGATTCCATGGTTCCCTCCAGTATACTAGCCTGRCTTGTAATGAATAATGA ATATTTCTTGATATTTÄATGKATAGGRCMTTTATTTATACTCAAT AATATTTTTCMAAAGGAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAA
Klon 0107-ContigClone 0107 contig
AGGGCGGCGGCCTTGGGCCGCGGGGAATGGGAGTCCCAGGGCCCTGACTGAGCACCGCCCCCGCCTCCCGGCCGCGCCATAGGGCGGCGGCCTTGGGCCGCGGGGAATGGGAGTCCCAGGGCCCTGACTGAGCACCGCCCCCGCCTCCCGGCCGCGCCAT
GGCTCAGGAGAAGATGGAGCTGGACCTGGAGTTGCCCGCGGGCGCGAGCCCGGCGGAGGGCGGCGGCCCTGGCGGCGGGGGGCTCAGGAGAAGATGGAGCTGGACCTGGAGTTGCCCGCGGGCGCGAGCCCGGCGGAGGGCGGCGGCCCTGGCGGCGGGG
GCCTGCGGAGGTCTAACAGCGCCCCCCTGATCCACGGCCTCAGCGACTCGTCGCCGGTGTTCCAGGCCGAGGCGCCCAGCGCCTGCGGAGGTCTAACAGCGCCCCCCTGATCCACGGCCTCAGCGACTCGTCGCCGGTGTTCCAGGCCGAGGCGCCCAGC
GCCAGACGGAACAGCACGACGTTCCCGAGCCGCCACGGCCTGCTGCTCCCGGCCTCGCCGGTCCGAATGCACAGCAGCCGGCCAGACGGAACAGCACGACGTTCCCGAGCCGCCACGGCCTGCTGCTCCCGGCCTCGCCGGTCCGAATGCACAGCAGCCG
CCTGCACCAGATCAAACAGGAGGAGGGCATGGACCTGATCAACCGAGAGACCGTTCACGAGCGCGAGGTGCAGACCGCAACCTGCACCAGATCAAACAGGAGGAGGGCATGGACCTGATCAACCGAGAGACCGTTCACGAGCGCGAGGTGCAGACCGCAA
TGCAGATAAGCCACTCCTGGGAGGAAAGTTTCAGCCTGAGTGACAACGACGTGGAGAAATCCGCCTCCCCGAAGCGCATCTGCAGATAAGCCACTCCTGGGAGGAAAGTTTCAGCCTGAGTGACAACGACGTGGAGAAATCCGCCTCCCCGAAGCGCATC
GATTTCATTCCGGTGTCTCCGGCACCGTCCCCGACCCGGGGAATTGGGAAGCAGTGTTTCTCACCGTCCTTGCAAAGTTTGATTTCATTCCGGTGTCTCCGGCACCGTCCCCGACCCGGGGAATTGGGAAGCAGTGTTTCTCACCGTCCTTGCAAAGTTT
TGTGAGTAGCAACGGACTGCCTCCAAGTCCCATTCCCAGCCCAACCACCCGATTTACCACCCGGAGAAGCCAGAGTCCCATGTGAGTAGCAACGGACTGCCTCCAAGTCCCATTCCCAGCCCAACCACCCGATTTACCACCCGGAGAAGCCAGAGTCCCA
TCAACTGCATTAGACCAAGTGTTCTTGGACCATTGAAAAGAAAATGTGAAATGGAAACTGATTATCAGCCAAAGAGATTTTCAACTGCATTAGACCAAGTGTTCTTGGACCATTGAAAAGAAAATGTGAAATGGAAACTGATTATCAGCCAAAGAGATTT
TTCCAGGGCATCACCAACATGCTGTCTTCTGACGTTGCACAGCTGTCAGACCCCGGTGTGTGCGTATCTTCCGATACCCTTTCCAGGGCATCACCAACATGCTGTCTTCTGACGTTGCACAGCTGTCAGACCCCGGTGTGTGCGTATCTTCCGATACCCT
GGATGGAAACAGCAGCAGTGCCGGATCTTCCTGTAACTCACCAGCGAAAGTCAGCACTACCACCGACTCTCCTGTGTCGCGGATGGAAACAGCAGCAGTGCCGGATCTTCCTGTAACTCACCAGCGAAAGTCAGCACTACCACCGACTCTCCTGTGTCGC
CCGCCCAAGCAGCCTCCCCCTTTATTCCAGTAGATGAACTTTCCTCAAAGTGACTCACTCATCTTGAATCCCCCTCCCCCCCGCCCAAGCAGCCTCCCCCTTTATTCCAGTAGATGAACTTTCCTCAAAGTGACTCACTCATCTTGAATCCCCCTCCCCC
AATGGAGAGACAAAAAAACAAAAACAAACAAAAAAAAAAAAAAAAATGGAGAGACAAAAAAACAAAAACAAACAAAAAAAAAAAAAAA
Klon 0111-ContigClone 0111 contig
AGGCAATGGCGGACGTGTCTGAGAGGACGCTGCAGGTGTCCGTGCTAGTGGCTTTCGCCTCTGGAGTGGTCCTGGGCTGGAGGCAATGGCGGACGTGTCTGAGAGGACGCTGCAGGTGTCCGTGCTAGTGGCTTTCGCCTCTGGAGTGGTCCTGGGCTGG
CAAGCGAATCGGCTGCGGAGGCGTTACCTAGACTGGAGGAAGCGGAGGCTGCAGGACAAGCTGGCAACGACTCAGAAAAACAAGCGAATCGGCTGCGGAGGCGTTACCTAGACTGGAGGAAGCGGAGGCTGCAGGACAAGCTGGCAACGACTCAGAAAAA
GCTGGACCTGGCCTGAGCACGCGCTGCAGCCCGAGTCCGCCGGGTTCTCACTCCCTAAGCCCAACGCAGCCCGGATCGTGGCTGGACCTGGCCTGAGCACGCGCTGCAGCCCGAGTCCGCCGGGTTCTCACTCCCTAAGCCCAACGCAGCCCGGATCGTG
GGAGCCGCGCGACCCAGGAGTCGTCCTTGCACGGCTTGCAAGAACATGGCTTGCTTCAGAAAGAAAATAGTTTTGTCTTCGGAGCCGCGCGACCCAGGAGTCGTCCTTGCACGGCTTGCAAGAACATGGCTTGCTTCAGAAAGAAAATAGTTTTGTCTTC
TCTAACAACTTACTTTCAGCTTGTCGAAGATGAAAATAAAAAGCACTGGAGAGAAATAATTTCTTGCACTTTATGAATCTTCTAACAACTTACTTTCAGCTTGTCGAAGATGAAAATAAAAAGCACTGGAGAGAAATAATTTCTTGCACTTTATGAATCT
ATTTTTAAAATAAAAAAATTAAMCATCAAAAAAAAAAAAAAAAAAAAAAAAAAAATTTTTAAAATAAAAAAATTAAMCATCAAAAAAAAAAAAAAAAAAAAAAAAAAA
Klon 0112-ContigClone 0112 contig
AGGGACTTTGTTCAGATCTTTTTCTGATCGTGCATCAGCTGCTGGTTCATTATAGCCTTCTCTTAAGCACTGAATAAGACAGGGACTTTGTTCAGATCTTTTTCTGATCGTGCATCAGCTGCTGGTTCATTATAGCCTTCTCTTAAGCACTGAATAAGAC
AAAAAATGAATCTTGGAAGAACAAATTCAGACATCATCAGTAAGTCTTTGGGGACACAGGGAATATTTGAACTTGATTTAAAAAAATGAATCTTGGAAGAACAAATTCAGACATCATCAGTAAGTCTTTGGGGACACAGGGAATATTTGAACTTGATTTA
ATCTGATGTCTTCTACAAAACCCGCTCTCCCGCATTACGTTGCTGTCCCCGCAGTCGCAGGCGCCCCCGGCCTGGCTGCGATCTGATGTCTTCTACAAAACCCGCTCTCCCGCATTACGTTGCTGTCCCCGCAGTCGCAGGCGCCCCCGGCCTGGCTGCG
GAACATGTTGAAGTCGTGTCCAGTGTGGTCGCCCTGGTGGAAGCACTCCGCGCACAGCGACATGCAGGGGGAGATGCCGCGAACATGTTGAAGTCGTGTCCAGTGTGGTCGCCCTGGTGGAAGCACTCCGCGCACAGCGACATGCAGGGGGAGATGCCGC
ACGTCCGGCAGCGGTAGGCCACGAAGTTGGCCGTCCAGACCAGGCCGCAGAGCGCCGCGGGGTCGTAGGCCCGCACGGCCACGTCCGGCAGCGGTAGGCCACGAAGTTGGCCGTCCAGACCAGGCCGCAGAGCGCCGCGGGGTCGTAGGCCCGCACGGCC
GCGCAGAACTCCTCGTAGCCGCCGCCGCCGGCCAGAAGGCACTTACACCACTCCAGGGCATCCTCCTCCGCCTCGCCCGGGCGCAGAACTCCTCGTAGCCGCCGCCGCCGGCCAGAAGGCACTTACACCACTCCAGGGCATCCTCCTCCGCCTCGCCCGG
GCCGCCGCCGCCGCCGCCCGCCGCCTCCTCGCCGCCCGCAGCCCCGGCCAGC Klon 0113-T7-SequenzGCCGCCGCCGCCGCCGCCCGCCGCCTCCTCGCCGCCCGCAGCCCCGGCCAGC Clone 0113-T7 sequence
AGGCGACGCGCGCATGGAGGCCGGCTGAGGAGCGCCGCCGCCTCTCTCGGTAAGGACTGTGTCTGTGTCCCCAGGCATCCAGGCGACGCGCGCATGGAGGCCGGCTGAGGAGCGCCGCCGCCTCTCTCGGTAAGGACTGTGTCTGTGTCCCCAGGCATCC
TACATCAATCAGGAAGCTGCTGTCCAGCCATGGAGGGAGAGGAGAAGCCAGCTCAAGAGGCTGACGTGGAACCTGTGGTATACATCAATCAGGAAGCTGCTGTCCAGCCATGGAGGGAGAGGAGAAGCCAGCTCAAGAGGCTGACGTGGAACCTGTGGTA
ACAGCAGGCACCTCAGAAGCAGTGCCAAGGGTGCTTGCTGGAGACCCTCAGAACATCTCTGATGTGGATGCCTTCAACTTACAGCAGGCACCTCAGAAGCAGTGCCAAGGGTGCTTGCTGGAGACCCTCAGAACATCTCTGATGTGGATGCCTTCAACTT
GCTCCTGGAGATGAAACTGAAACGACGGCGTGAACGCCCCAACCTTCCACGTACAGTGACCCAGCTAGTGGCCGAGGATGGCTCCTGGAGATGAAACTGAAACGACGGCGTGAACGCCCCAACCTTCCACGTACAGTGACCCAGCTAGTGGCCGAGGATG
GGAGCAGGGTGTATGTGGTGGGCACTGCTCACTTCAGTGATGATAGCAAGAGAGATGTAGTAAAGACTATCCGGGAGGTGGGAGCAGGGTGTATGTGGTGGGCACTGCTCACTTCAGTGATGATAGCAAGAGAGATGTAGTAAAGACTATCCGGGAGGTG
CAACCGGATGTGGTCGTGGNGGAGCTCTGTCAGTACCGGGGTGTCCATGCTCAAGATGGACGAGAGGACGCTGCTGCGAGCAACCGGATGTGGTCGTGGNGGAGCTCTGTCAGTACCGGGGTGTCCATGCTCAAGATGGACGAGAGGACGCTGCTGCGAG
AGGCCATGGAGGTCAGCCTGGAGAAGCTGCAGCAGGCTGTCAGGCAGAATGGNTTATGTCTGGACTCATGAGATGTTGCTAGGCCATGGAGGTCAGCCTGGAGAAGCTGCAGCAGGCTGTCAGGCAGAATGGNTTATGTCTGGACTCATGAGATGTTGCT
GCNTGNAGGTGTCTGCTCACATCACTGAGCAGCTGGGCATGGCCCCTGGTGNCGAGTTCAGGGAGGCCTTCAAAGAGGCCGCNTGNAGGTGTCTGCTCACATCACTGAGCAGCTGGGCATGGCCCCTGGTGNCGAGTTCAGGGAGGCCTTCAAAGAGGCC
AGCAAGGTACCATTCTGCAAATTCCACCTGGGNGACCGACCAATCCCAGNCAGCAAGGTACCATTCTGCAAATTCCACCTGGGNGACCGACCAATCCCAGNC
Klon 0115-T7-SequenzClone 0115-T7 sequence
AGCCCGTCCCCAGCGCTCCGGCGAGCTGGCCTCCGCTGCAGTTGAGCCGGGCTCGCACTATGCAGCGCCTGGCCATGGACAGCCCGTCCCCAGCGCTCCGGCGAGCTGGCCTCCGCTGCAGTTGAGCCGGGCTCGCACTATGCAGCGCCTGGCCATGGAC
CTGCGGGTGCTGTCCCGGGAGCTGGCGCTCTACCTGGAGCACCAGGTTCGCGTCGGCTTCTTCGGCTCGGGCGTGGGCTTCTGCGGGTGCTGTCCCGGGAGCTGGCGCTCTACCTGGAGCACCAGGTTCGCGTCGGCTTCTTCGGCTCGGGCGTGGGCTT
GTCACTGATCTTGGGATTCAGCGTTGCCTATGCTTGCTACTACCTGAGTAGCATTGCCAAGAAACCCCAGTTAGTGATTGGTCACTGATCTTGGGATTCAGCGTTGCCTATGCTTGCTACTACCTGAGTAGCATTGCCAAGAAACCCCAGTTAGTGATTG
GAGGGGAGAGTTTCAGCNCGCTTCCTACAGGACCACTGTCCCGTGGTGACAGAAACCTATTACCCGACGGTCTGGTGCTGGAGGGGAGAGTTTCAGCNCGCTTCCTACAGGACCACTGTCCCGTGGTGACAGAAACCTATTACCCGACGGTCTGGTGCTG
GGAGAGCCGAGGACAGACATTGCTGAGACCCTTCÄTCACTTCCAAACCCCCGGTGCAATACAGGAACGAACTCATTAAAAGGAGAGCCGAGGACAGACATTGCTGAGACCCTTCÄTCACTTCCAAACCCCCGGTGCAATACAGGAACGAACTCATTAAAA
CTGCAGACGGAGGACAGATCTCACTGGACTGGTTTGATAACAATAACAGTGCGTACTATGNGGATGCCAGCACCAGACCNCTGCAGACGGAGGACAGATCTCACTGGACTGGTTTGATAACAATAACAGTGCGTACTATGNGGATGCCAGCACCAGACCN
TACTATCTTGCTCTTGCCTGGCCTNACTGNAACAAGCAAGGAATCCTACATCCTTCATATGATCCATCTCAGTGAAGAATTACTATCTTGCTCTTGCCTGGCCTNACTGNAACAAGCAAGGAATCCTACATCCTTCATATGATCCATCTCAGTGAAGAAT
TNGGATACAGGTGNGNGGNTTTTAATAACANAGGAGTAGCANGAGAAAGTCTCTTGACACCACCGGACTTACTGCTGNGCTNGGATACAGGTGNGNGGNTTTTAATAACANAGGAGTAGCANGAGAAAGTCTCTTGACACCACCGGACTTACTGCTGNGC
GAACACTGAAGAACTTGGAGGCCGNNGTCCACCACGTGCACAGCCTGTGAACACTGAAGAACTTGGAGGCCGNNGTCCACCACGTGCACAGCCTGT
Klon 0116-ContigClone 0116 contig
AGGGCGCAGCCGGCCTAGCGAGGTCAACATGCCGGTCGCCAGAAGCTGGGTTTGTCGCAAAACCTATGTGACCCCACGGAAGGGCGCAGCCGGCCTAGCGAGGTCAACATGCCGGTCGCCAGAAGCTGGGTTTGTCGCAAAACCTATGTGACCCCACGGA
GACCCTTCGAGAAGTCGCGTCTCGACCAGGAGCTAAAGTTGATTGGAGAGTATGGACTCCGGAACAAACGTGAGGTTTGGGACCCTTCGAGAAGTCGCGTCTCGACCAGGAGCTAAAGTTGATTGGAGAGTATGGACTCCGGAACAAACGTGAGGTTTGG
AGGGTCAAGTTTACCCTGGCCAAGATCCGTAAGGCGGCCCGGGAGCTGTTGACGCTGGACGAGAAGGATCCCCGGCGTCTAGGGTCAAGTTTACCCTGGCCAAGATCCGTAAGGCGGCCCGGGAGCTGTTGACGCTGGACGAGAAGGATCCCCGGCGTCT
GTTTGAAGGCAATGCTCTCCTGCGGCGGCTTGTTCGCATTGGGGTGCTGGACGAGGGCAAGATGAAGCTGGATTACATCCGTTTGAAGGCAATGCTCTCCTGCGGCGGCTTGTTCGCATTGGGGTGCTGGACGAGGGCAAGATGAAGCTGGATTACATCC
TGGGACTGAAGATTGAGGATTTCTTGGAGAGGCGGCTGCAGACCCAGGTCTTTAAGCTGGGCCTGGCCAAATCTATTCACTGGGACTGAAGATTGAGGATTTCTTGGAGAGGCGGCTGCAGACCCAGGTCTTTAAGCTGGGCCTGGCCAAATCTATTCAC
CATGCCCGTGTGCTCATCCGCCAACGTCACATTAGGGTCCGCAAGCAGGTGGTGAACATCCCATCCTTTATTGTTCGCCTCATGCCCGTGTGCTCATCCGCCAACGTCACATTAGGGTCCGCAAGCAGGTGGTGAACATCCCATCCTTTATTGTTCGCCT
GGACTCGCAGAAGCACATCGACTTCTCCCTCCGTTCTCCTTATGGTGGCGGCCGTCCAGGCCGAGTGAAGAGGAAGAATGGGACTCGCAGAAGCACATCGACTTCTCCCTCCGTTCTCCTTATGGTGGCGGCCGTCCAGGCCGAGTGAAGAGGAAGAATG
CCAAGAAAGGCCAGGGCGGGGCTGGAGCTGGTGATGATGAGGAAGAGGATTAATTCTTGGCTGAACTGGAGGATTGTCTACCAAGAAAGGCCAGGGCGGGGCTGGAGCTGGTGATGATGAGGAAGAGGATTAATTCTTGGCTGAACTGGAGGATTGTCTA
GTTTTCCAGCTGAAAAATAAAAAAGAATTGATACTTGGAAAAAAAAAAAAAAAAAAAAGTTTTCCAGCTGAAAAATAAAAAAGAATTGATACTTGGAAAAAAAAAAAAAAAAAAAA
Klon 0117-T7-SequenzClone 0117-T7 sequence
AGCGGCGGCTCTGAGTCGCCGGAGCCGCCCCGGGACÄTCATGGCGGAGGTCTGCGAGCCCACCCGCCCCTCGGAGGACGAAGCGGCGGCTCTGAGTCGCCGGAGCCGCCCCGGGACÄTCATGGCGGAGGTCTGCGAGCCCACCCGCCCCTCGGAGGACGA
GGACGAGGAGCGGGAGCCGCTGCTGCCTCGCGTTGCCTGGGCCCAGCCGCGGAGGGTCGCGCCCGGGAGCGCCGTGAGAAGGACGAGGAGCGGGAGCCGCTGCTGCCTCGCGTTGCCTGGGCCCAGCCGCGGAGGGTCGCGCCCGGGAGCGCCGTGAGAA
TGCAGGCGGACGAGGGCGCGGATGTCCTCCGCGAGCCCGCTACCGACGAGCCGCCGGCGGTGTCCGGGGAAGGGTCGATCTGCAGGCGGACGAGGGCGCGGATGTCCTCCGCGAGCCCGCTACCGACGAGCCGCCGGCGGTGTCCGGGGAAGGGTCGATC
TCCGCGAGCTTGTCCACCGAGCTGGATGGGACCCGCACTACGAGCTCAGAAACAAATACATTCTTGGAAGATCCAGAATTTCCGCGAGCTTGTCCACCGAGCTGGATGGGACCCGCACTACGAGCTCAGAAACAAATACATTCTTGGAAGATCCAGAATT
TGCTGATATTGTATTGAAAGCAGAGCAAGCAATAGAAATTGGAGTTTTCCCAGAAAGAATTTCTTGCTGATATTGTATTGAAAGCAGAGCAAGCAATAGAAATTGGAGTTTTCCCAGAAAGAATTTCT
Klon 0119-ContigClone 0119 contig
CATCATCGAGACAAGCACCATTAAGCCTTACCGTCGAGGGTTTTACTGCAATGACGAGAGCATCAAGTATCCCCTGAAAGCATCATCGAGACAAGCACCATTAAGCCTTACCGTCGAGGGTTTTACTGCAATGACGAGAGCATCAAGTATCCCCTGAAAG
TCAGTGAGACTATAAACGATGCTGNGCTCTGNGCGGNGGGGATCGTCATCGCCATCCTGGCGATCATTACAGGGGAATTCTCAGTGAGACTATAAACGATGCTGNGCTCTGNGCGGNGGGGATCGTCATCGCCATCCTGGCGATCATTACAGGGGAATTC
TACCGGATCTATTACCTCAAGGAGAAGTCCCGCTCCACCACTCAGAACCCGTATGTGGCAGCNCNCNATAAGCAAGTGGNTACCGGATCTATTACCTCAAGGAGAAGTCCCGCTCCACCACTCAGAACCCGTATGTGGCAGCNCNCNATAAGCAAGTGGN
ATGCNTCCTTTNNGNTGTGCCAATTAGCAAGTCCTTCANNGNCATCGCCAAAGTGTCCATCGGGCGCCTAAGGCCTCACTATGCNTCCTTTNNGNTGTGCCAATTAGCAAGTCCTTCANNGNCATCGCCAAAGTGTCCATCGGGCGCCTAAGGCCTCACT
TCCTNAGCGTCTGTGACCCTGATTTCAGTCAGATCAATTGCTCCGAGGGCTACATTCAGANCTACAGGTGCAGAGGAGAATCCTNAGCGTCTGTGACCCTGATTTCAGTCAGATCAATTGCTCCGAGGGCTACATTCAGANCTACAGGTGCAGAGGAGAA
GNCAGCANAGTACAGGAGGCCAGGAAGTCCTTCTTCTCGGGCCACGCCTCCTTCTCCATGTTCACTATGCTGTATCTGGTGNCAGCANAGTACAGGAGGCCAGGAAGTCCTTCTTCTCGGGCCACGCCTCCTTCTCCATGTTCACTATGCTGTATCTGGT
GCTCTACCTTCAGGCCCGCTTCACCTGGCGCGGGGCCCGACTGCTCCGCCCCCTCCTGCAGTTCACTTTGCTCATGATGGGCTCTACCTTCAGGCCCGCTTCACCTGGCGCGGGGCCCGACTGCTCCGCCCCCTCCTGCAGTTCACTTTGCTCATGATGG
CCTTCTACACGGGATTGTCACGGGTATCTGACTACAAGCATCATCCTAGCGATGTCCTGGCAGGATTTGCCCAAGGAGCTCCTTCTACACGGGATTGTCACGGGTATCTGACTACAAGCATCATCCTAGCGATGTCCTGGCAGGATTTGCCCAAGGAGCT
CTGGTGGCCTGCTGCATAGTGTTCTTCGTGTCCGACCTCTTCAAGACTAAGACGAGCCTCTCACTGCCCGCCCCTGCGATCTGGTGGCCTGCTGCATAGTGTTCTTCGTGTCCGACCTCTTCAAGACTAAGACGAGCCTCTCACTGCCCGCCCCTGCGAT
CAGGAGGGAGATCCTGTCTCCCGTGGACATCATCGACAGGÄACAATCACCATAACATGGTGTAGATGCTGCGGCCTCCGGCAGGAGGGAGATCCTGTCTCCCGTGGACATCATCGACAGGÄACAATCACCATAACATGGTGTAGATGCTGCGGCCTCCGG
AGCGCTTTCTCTGAAGCGACTGCACGTTCCTGCTGCTCTCCGATCTCATCAGACAGTAGAATGTAGGGAAAAGCTTTTGCAGCGCTTTCTCTGAAGCGACTGCACGTTCCTGCTGCTCTCCGATCTCATCAGACAGTAGAATGTAGGGAAAAGCTTTTGC
CCGATGGATTTTGAAAACATTTAAAAAAAAAAAAAAAAACCGATGGATTTTGAAAACATTTAAAAAAAAAAAAAAAAA
Klon 0121-BGH-SequenzClone 0121 BGH sequence
TGCAGGGAAGACAGAAAGGCCTCCCAGGCCACTTGGTTTATTAGATCCTGAAGAGAGGTGTAGGCAGTGCCCCTGGGCCG CTGCCACCTCCTGGGGGAGGACCTGTGGGAGGCACAGGGCCGAACCTCGTTTTGATACACACAACCCCATTTGAGGGAAA ACAGGCTGCTTCGAAGCCTGAGGGATGGTGAGGGGTGATGCCTGCCATACAGGAAGCCAGGTCCTGGGAGGGCACAAACG ATGAATCCATCACTGCCCCAGCTCTGCCAGCATGCCCACCTGGCCCTGGGGAAGCCAGGCAΆGGGAGGGCACAGGCGTGT GAGGGACACAGACAGTTCCTGGTGACGGCAGTAGCTGCTGAGCAGGAGGGTTCAGCAAAACTGACCATTAGAGCAGCCAA GGCTGCATATAGGAGGTGCGCTCGGGAACCCCAGGCACTTTCTCTGGACTCCACGGTCATGGCTTCTGCTGGTGATCTGC ACTGCCTGCCTGTCCCCTCTCCTGAAGGCACTACCTTCCAGAACACAGCACGGTGGTCCCTCTTGTGACAAAGTGCTTGT GTGTGTGCTCTAGTCATTTGGTAΆGCAGAAGCTGCCACGGGCCATACCCTGCCACACTACCCAAGTTCTGGGCNGGAAAC TGCTCCCTGCACAGAGGGCCAGCGGGAGCAGGAAACGAACTCAACTTCGCTGGGCTTGCCAGGCANGGCACGGTAGCAGC CAGGGAGGTTGGGACAGTGACÄNCAGNCAGGCAGACTTTTTTGTGCAGGGAAGACAGAAAGGCCTCCCAGGCCACTTGGTTTATTAGATCCTGAAGAGAGGTGTAGGCAGTGCCCCTGGGCCG CTGCCACCTCCTGGGGGAGGACCTGTGGGAGGCACAGGGCCGAACCTCGTTTTGATACACACAACCCCATTTGAGGGAAA ACAGGCTGCTTCGAAGCCTGAGGGATGGTGAGGGGTGATGCCTGCCATACAGGAAGCCAGGTCCTGGGAGGGCACAAACG ATGAATCCATCACTGCCCCAGCTCTGCCAGCATGCCCACCTGGCCCTGGGGAAGCCAGGCAΆGGGAGGGCACAGGCGTGT GAGGGACACAGACAGTTCCTGGTGACGGCAGTAGCTGCTGAGCAGGAGGGTTCAGCAAAACTGACCATTAGAGCAGCCAA GGCTGCATATAGGAGGTGCGCTCGGGAACCCCAGGCACTTTCTCTGGACTCCACGGTCATGGCTTCTGCTGGTGATCTGC ACTGCCTGCCTGTCCCCTCTCCTGAAGGCACTACCTTCCAGAACACAGCACGGTGGTCCCTCTTGTGACAAAGTGCTTGT GTGTGTGCTCTAGTCATTTGGTAΆGCAGAAGCTGCCACGGGCCATACCCTGCCACACTACCCAAGTTCTGGGCNGGAAAC TGCTCCCTGCACAGAGGGCCAGCGGGAGCAGGAAACGAACTCAACTTCGCTGGGCTTGCCAGGCANGGCACGGTAGCAGC CAGGGAGGTTGGGACAGTGACÄNCAGNCAGGCAGACTTTTTTG
Klon 0123-T7-SequenzClone 0123-T7 sequence
AGCAACCGCAACCTGACCTTGACCAAGAAGGAACCTGTTGGGGTCTGTGGTATTGTCATCCCCTGGAACTATCCCTTAAT GATGCTGTCCTGGAAGACAGCAGCCTGCCTGGCTGCCGGG ACACCGTGKTGATCAΆGCCTGCCCAGGTGACCCCACTCA CAGCCTTGAAGTTTGCAGAGCTGACACTGAAGGCTGGCATTCCCAAGGGTGTGGTCAACATCCTCCCAGGATCTGGCTCG CTGGTTGGCCAGAGACTCTCAGACCACCCTGATGTGAGGAAAATAGGGTTCACAGGCTCCACGGAGGTGGGAAAACACAT CATGAAAAGCTGTGCCCTGAGTAATGTGAAGAΆGGTCTCCCTGGAGCTGGGTGGAAAGTCACCCCTTATCATCTTTGCTG ACTGTGACCTCAACAAAGCTGAGCAACCGCAACCTGACCTTGACCAAGAAGGAACCTGTTGGGGTCTGTGGTATTGTCATCCCCTGGAACTATCCCTTAAT GATGCTGTCCTGGAAGACAGCAGCCTGCCTGGCTGCCGGG ACACCGTGKTGATCAΆGCCTGCCCAGGTGACCCCACTCA CAGCCTTGAAGTTTGCAGAGCTGACACTGAAGGCTGGCATTCCCAAGGGTGTGGTCAACATCCTCCCAGGATCTGGCTCG CTGGTTGGCCAGAGACTCTCAGACCACCCTGATGTGAGGAAAATAGGGTTCACAGGCTCCACGGAGGTGGGAAAACACAT CATGAAAAGCTGTGCCCTGAGTAATGTGAAGAΆGGTCTCCCTGGAGCTGGGTGGAAAGTCACCCCTTATCATCTTTGCTG ACTGTGACCTCAACAAAGCTG
Klon 0124-ContigClone 0124 contig
TGGAAAGGCGATGGGCATCTCTCGGGACAACTGGCACAAGCGCCGCAAGACCGGGGGTAAGCGAAAACCCTACCACAAGA AGCGAAAGTATGAGCTGGGACGGCCCGCTGCCAACACGAAGATTGGCCCTCGCCGCATACACACAGTCCGAGTTCGAGGA GGCAATAAGAAGTACCGTGCCCTGAGATTGGATGTGGGGAACTTTTCCTGGGGCTCTGAGTGTTGTACTCGCAAAACAAG GATCATTGATGTTGTCTACAATGCATCCAACAACGAGCTTGTCCGCACCAAGACCCTGGTGAAGAACTGCATCGTGCTTA TTGACAGCACGCCGTACCGACAGTGGTACGAGTCCCACTATGCACTGCCCCTGGGCCGCAAGAAGGGGGCCAAGCTGACT CCTGAGGAGGAAGAGATTTTAACAAAAAACGATCAAAGAAAATTCAAAAGAAAT CGATGAAAGGAAAAAGAATGCCAΆ ATCAGCAGCCTCCTGGAGGAGCAGTTCCAGCAGGGCAAGCTTYTAGCCTGTATTGCCTCMCGACCAGGCCAGTGTGGCA GAGCAGACGGCTΆTGTGCTCGAAGGCAAGGAGCTGGAGTTCTATYTGCGGAAGATCAAAGCCCGGAAAGGCAATGAGCC TCATGTGTAGTGTAATAAAGGTGTYTGCTGTTCTATYTTAAAAAAAAAAAAAAAAAAAAAAATGGAAAGGCGATGGGCATCTCTCGGGACAACTGGCACAAGCGCCGCAAGACCGGGGGTAAGCGAAAACCCTACCACAAGA AGCGAAAGTATGAGCTGGGACGGCCCGCTGCCAACACGAAGATTGGCCCTCGCCGCATACACACAGTCCGAGTTCGAGGA GGCAATAAGAAGTACCGTGCCCTGAGATTGGATGTGGGGAACTTTTCCTGGGGCTCTGAGTGTTGTACTCGCAAAACAAG GATCATTGATGTTGTCTACAATGCATCCAACAACGAGCTTGTCCGCACCAAGACCCTGGTGAAGAACTGCATCGTGCTTA TTGACAGCACGCCGTACCGACAGTGGTACGAGTCCCACTATGCACTGCCCCTGGGCCGCAAGAAGGGGGCCAAGCTGACT CCTGAGGAGGAAGAGATTTTAACAAAAAACGATCAAAGAAAATTCAAAAGAAAT CGATGAAAGGAAAAAGAATGCCAΆ ATCAGCAGCCTCCTGGAGGAGCAGTTCCAGCAGGGCAAGCTTYTAGCCTGTATTGCCTCMCGACCAGGCCAGTGTGGCA GAGCAGACGGCTΆTGTGCTCGAAGGCAAGGAGCTGGAGTTCTATYTGCGGAAGATCAAAGCCCGGAAAGGCAATGAGCC TCATGTGTAGTGTAATAAAGGTGTYTGCTGTTCTATYTTAAAAAAAAAAAAAAAAAAAAAAA
Klon 0125-ContigClone 0125 contig
CTGGAAAGCCHGGATGAGGCTCTTCATCGCTCTTCCTGTCCTGATTGTGGTCGTAGCCATGACCTTGGAAGGCCCAGCCCCTGGAAAGCCHGGATGAGGCTCTTCATCGCTCTTCCTGTCCTGATTGTGGTCGTAGCCATGACCTTGGAAGGCCCAGCCC
CCGCCCAGGCGGCCCCGGATTTGTCCGGAACATTGGAGAGCATACCGGATAÄACTGAAGGAGTTTGGGAACACTTTGGAACCGCCCAGGCGGCCCCGGATTTGTCCGGAACATTGGAGAGCATACCGGATAÄACTGAAGGAGTTTGGGAACACTTTGGAA
GACAAGGCCCGGGCAGCCATTGAACATATCAAACAGAAGGAAATTTTGACCAAGACCCGGGCCTGGTTCTCAGAGGCATTGACAAGGCCCGGGCAGCCATTGAACATATCAAACAGAAGGAAATTTTGACCAAGACCCGGGCCTGGTTCTCAGAGGCATT
TGGCAAAGTGAAGGAGAAGTTGAAGACCACGTTCTCCTGAGCACCTGGCGGGCCACCTCGAAGCATCAAGGACATCCACGTGGCAAAGTGAAGGAGAAGTTGAAGACCACGTTCTCCTGAGCACCTGGCGGGCCACCTCGAAGCATCAAGGACATCCACG
TAATGTGCCAGGTCCCTCTTCATCACAGACCAATAAAAAACGTGTAGAAGGCAAAATAATGTGCCAGGTCCCTCTTCATCACAGACCAATAAAAAACGTGTAGAAGGCAAAA
Klon 0127-ContigClone 0127 contig
AGGTTTGAACAGCTTTCTGAATCTGCAAAAGAAGAGCTGATAAACTTCAAACGGAAGAGAGTGGCAGCATTTCGAAAGAAAGGTTTGAACAGCTTTCTGAATCTGCAAAAGAAGAGCTGATAAACTTCAAACGGAAGAGAGTGGCAGCATTTCGAAAGAA
CCTAATCGAAATGTCTGAACTGGAAATAAAGCATGCCAGAAACAACGTCTCCCTGTTGCAGAGCTGCATCGACTTATTCACCTAATCGAAATGTCTGAACTGGAAATAAAGCATGCCAGAAACAACGTCTCCCTGTTGCAGAGCTGCATCGACTTATTCA
AGAACAACTGACCTGTCTAYTTTAAAGGCCCCCAWTGTGAAAGCCAGCATCACTTGCACTTAAATCATTACTGCAAAAGAAGAACAACTGACCTGTCTAYTTTAAAGGCCCCCAWTGTGAAAGCCAGCATCACTTGCACTTAAATCATTACTGCAAAAGA
AATAGCTTTGACTAGTTTAAAATCATGTGAATAAATATTTTGATTTCTAAAAATCTTAACACTTAACCATGTTGGTTTAAAATAGCTTTGACTAGTTTAAAATCATGTGAATAAATATTTTGATTTCTAAAAATCTTAACACTTAACCATGTTGGTTTAA
AAATATTTTCATTGCATGTTACTTGGACATAACTAATCCTTTTCTTATGCATTTAATACCTCAAAGTAGGTAGAATCTAAAAATATTTTCATTGCATGTTACTTGGACATAACTAATCCTTTTCTTATGCATTTAATACCTCAAAGTAGGTAGAATCTAA
CAGATTCTCCTATAGACTGTCAGGAATTACTAAGAAATGGTATGGANATGTGTGCCTTCTGGAAATGAGAAGCAATAGCCCAGATTCTCCTATAGACTGTCAGGAATTACTAAGAAATGGTATGGANATGTGTGCCTTCTGGAAATGAGAAGCAATAGCC
TGGCCCATGTCTGTCTGAACATTTCCTGTGTCTGCTCCTACAGACATGTCAGGTGTGAATTACAGCAAACAGTGACTGCTTGGCCCATGTCTGTCTGAACATTTCCTGTGTCTGCTCCTACAGACATGTCAGGTGTGAATTACAGCAAACAGTGACTGCT
TGTGGGCAGGTGGCAGACACTCTTCTTTATTTAGGTANATTTTGCTCCCTCTTGTCAAGATCACTTCANATGCTTGTACGTGTGGGCAGGTGGCAGACACTCTTCTTTATTTAGGTANATTTTGCTCCCTCTTGTCAAGATCACTTCANATGCTTGTACG
GTAACAAGCAAGAAGATCTCATATGTCTTTTTACATCCTTTACAGCTCCATTTTTGTAACTGGGATATAAATACTTGAGAGTAACAAGCAAGAAGATCTCATATGTCTTTTTACATCCTTTACAGCTCCATTTTTGTAACTGGGATATAAATACTTGAGA
GAAAGGAGAATCTANAGTTNTGACACTCCCTGATGGTGGGGCATGTGTGTTTGTGCTTTGGATGTTTTTATAAAGTATATGAAAGGAGAATCTANAGTTNTGACACTCCCTGATGGTGGGGCATGTGTGTTTGTGCTTTGGATGTTTTTATAAAGTATAT
AATAAAGATAATCCATGTTAATAAAGATAATCCATGTT
Klon 0128-T7-SequenzClone 0128-T7 sequence
GGCGTGCTGACGTCAGGCGCGTGCCCCTGTCCGGCAGCCGAGGAGGCCCCGCGCAGTGCTGCCAACGCCCCGGCGGAGAA GCTGAGGACAAGATCTAATTTGAAATATTAAAAGTGGATACAAAACTGTTTCCGAAATGCAGACAATTAAGTGTGTTGTT GTTGGTGATGGTGCTGTTGGTAAAACATGTCTCCTGATATCCTACACAACAAACAAATTCCCATCGGAATATGTACCAAC TGTTTTTGACAACTATGCAGTCACAGTTATGATTGGTGGAGAGCCATACACTCTTGGACTTTTTGATACTGCAGGGCAAG AGGATTATGACAGACTACGACCGCTAAGTTATCCACAGACAGATGTTTTTCTAGTATGTTTCTCAGTGGTCTCTCCATCC TCATTTGAAAATGTGAAAGAAAAGTGGGTGCCTGAGATAACTCACCACTGTCCAAΆGACTCCTTTCTTGCTTGTTGGGAC CCAAATTGATCTCAGAGATGACCCCTCTACTATTGAGAAACTTGCCAAGACAAACAGAAGCGGCGTGCTGACGTCAGGCGCGTGCCCCTGTCCGGCAGCCGAGGAGGCCCCGCGCAGTGCTGCCAACGCCCCGGCGGAGAA GCTGAGGACAAGATCTAATTTGAAATATTAAAAGTGGATACAAAACTGTTTCCGAAATGCAGACAATTAAGTGTGTTGTT GTTGGTGATGGTGCTGTTGGTAAAACATGTCTCCTGATATCCTACACAACAAACAAATTCCCATCGGAATATGTACCAAC TGTTTTTGACAACTATGCAGTCACAGTTATGATTGGTGGAGAGCCATACACTCTTGGACTTTTTGATACTGCAGGGCAAG AGGATTATGACAGACTACGACCGCTAAGTTATCCACAGACAGATGTTTTTCTAGTATGTTTCTCAGTGGTCTCTCCATCC TCATTTGAAAATGTGAAAGAAAAGTGGGTGCCTGAGATAACTCACCACTGTCCAAΆGACTCCTTTCTTGCTTGTTGGGAC CCAAATTGATCTCAGAGATGACCCCTCTACTATTGAGAAACTTGCCAAGACAAACAGAAGC
Klon 0131-T7-Sequenz AGAGGGAGGCTCTCAGCGATACGTGCCCGGCCCAGCTTTTTTTTTCTTCAAAATGTCTACTGTCCACGAAATCCTGTGCA AGCTCAGCCTGGAGGGTGATCATTCTACACCCCCAAGTGCCTACGGGTCAGTCAAACCCTACACCAACTTCGATGCTGAG AGGGATGCTCTGAACATTGAGACAGCAGTCAAGACCAAAGGAGTGGATGAGGTCACCATTGTCAACATCCTGACAAACCG CAGCAATGTGCAGAGGCAGGACATTGCCTTCGCCTATCAGAGAAGGACCAAAAAGGAGCTCCCGTCAGCGCTGAAGTCAG CCTTATCTGGCCACCTGGAGACGGTGATTTTGGGCCTATTGAAGACACCTGCCCΆGTATGATGCTTCGGAACTΆAΆAGCT TCCATGAAGGGCCTGGGGACTGACGAGGACTCCCTCATTGAGATCATCTGCTCACGAACCAACCAGGAGCTGCAAGAGAT CAACAGAGTGTACAAGGAAATGTACAAGACTGATCTGGAGAΆGGACATCATCTCTGACACATCTGGAGACTTCCGAAAGC TGATGGTCGCCCTClone 0131-T7 sequence AGAGGGAGGCTCTCAGCGATACGTGCCCGGCCCAGCTTTTTTTTTCTTCAAAATGTCTACTGTCCACGAAATCCTGTGCA AGCTCAGCCTGGAGGGTGATCATTCTACACCCCCAAGTGCCTACGGGTCAGTCAAACCCTACACCAACTTCGATGCTGAG AGGGATGCTCTGAACATTGAGACAGCAGTCAAGACCAAAGGAGTGGATGAGGTCACCATTGTCAACATCCTGACAAACCG CAGCAATGTGCAGAGGCAGGACATTGCCTTCGCCTATCAGAGAAGGACCAAAAAGGAGCTCCCGTCAGCGCTGAAGTCAG CCTTATCTGGCCACCTGGAGACGGTGATTTTGGGCCTATTGAAGACACCTGCCCΆGTATGATGCTTCGGAACTΆAΆAGCT TCCATGAAGGGCCTGGGGACTGACGAGGACTCCCTCATTGAGATCATCTGCTCACGAACCAACCAGGAGCTGCAAGAGAT CAACAGAGTGTACAAGGAAATGTACAAGACTGATCTGGAGAΆGGACATCATCTCTGACACATCTGGAGACTTCCGAAAGC TGATGGTCGCCCT
Klon 0133-ContigClone 0133 contig
CTGGAAAGGCTCCTCGAGGGTCGGTCCCGNCACGTCTNTTCCCGGTCTCTCTGGCTCTGGAGTGCTTTCCCCCCGCGGAGCTGGAAAGGCTCCTCGAGGGTCGGTCCCGNCACGTCTNTTCCCGGTCTCTCTGGCTCTGGAGTGCTTTCCCCCCGCGGAG
GGTGGGAGCGCGGGGACAGACGGGCGAGATGAGCACCATGTTTGCAGACACGCTGCTCATCGNCTTTATCTCCGTGTGCAGGTGGGAGCGCGGGGACAGACGGGCGAGATGAGCACCATGTTTGCAGACACGCTGCTCATCGNCTTTATCTCCGTGTGCA
CCGCGCTGCTCGCCGAGGGCATAACCTGGGTCCTGGTTTACAGGACAGACAAATACAAGAGATTGAAGGCAGAAGTGGAACCGCGCTGCTCGCCGAGGGCATAACCTGGGTCCTGGTTTACAGGACAGACAAATACAAGAGATTGAAGGCAGAAGTGGAA
AAACARAGTRAAAAATTANAGAAGAAGAAGGAAACTATAACAGAGTCAGCTGGTCGACAACAGAAGAAGAAGATAGAGAGAAACARAGTRAAAAATTANAGAAGAAGAAGGAAACTATAACAGAGTCAGCTGGTCGACAACAGAAGAAGAAGATAGAGAG
ACAAGAAGAGAAATTAAAGAACAACAATAGAGACCTGTCAATGGTACGGATGAAATCCATGTTCGCGATTGGCTTTTG TACAAGAAGAGAAATTAAAGAACAACAATAGAGACCTGTCAATGGTACGGATGAAATCCATGTTCGCGATTGGCTTTTG T
TTACTGCCTTAATGGGAATGTTTAATTCCATATTTGATGGTAGAGTGGTGGCAAAGCTCCCCTTCACTCCTCTGTCTTACTTACTGCCTTAATGGGAATGTTTAATTCCATATTTGATGGTAGAGTGGTGGCAAAGCTCCCCTTCACTCCTCTGTCTTAC
ATCCAAGGACTATCTCATCGGAACCTGCTGGGAGATGACACCACGGACTGCTCCTTCATCTTCCTGTATATCCTCTGTACATCCAAGGACTATCTCATCGGAACCTGCTGGGAGATGACACCACGGACTGCTCCTTCATCTTCCTGTATATCCTCTGTAC
CATGTCAATTCGACAAAACATCCAGAAGATTCTTGGCCTCGCCCCTTCACGAGCTGCCACCAAGCAGGCTGGTGGATTTCCATGTCAATTCGACAAAACATCCAGAAGATTCTTGGCCTCGCCCCTTCACGAGCTGCCACCAAGCAGGCTGGTGGATTTC
TTTGGCCCACCACCTCCGTCTGGGAAGTTTTCCTGAAGGAAAGCAGAATTCTGAATTTCCTGTCATACTTTTTAGACATTTTTGGCCCACCACCTCCGTCTGGGAAGTTTTCCTGAAGGAAAGCAGAATTCTGAATTTCCTGTCATACTTTTTAGACATT
CACATCAGACTTACAGAGCACCTGGCCACATTCTAGGTTTGTGTTATTTCTCAGCTATGGATATGATCAAATGAGANCACCACATCAGACTTACAGAGCACCTGGCCACATTCTAGGTTTGTGTTATTTCTCAGCTATGGATATGATCAAATGAGANCAC
TGCTTAACTAATGGGATAGTGCTATGGTCACGGATGGCTTCTTTAGTAATAAGTGCACTCACTGGTTACATTTGGATGGCTGCTTAACTAATGGGATAGTGCTATGGTCACGGATGGCTTCTTTAGTAATAAGTGCACTCACTGGTTACATTTGGATGGC
TTATGTAACCACAAACATCATCTTTCTTGTCTCACTTGAGTATTTCAGTGTATGTGCCATATACACAGAAGCAGACTGTCTTATGTAACCACAAACATCATCTTTCTTGTCTCACTTGAGTATTTCAGTGTATGTGCCATATACACAGAAGCAGACTGTC
CATGAACATTAGGAGAAAAATAGTTTATGTAACAACTTCACTGTAATTAGTTGTTGTGTTTTTTAAAAAGCCAAATATGTCATGAACATTAGGAGAAAAATAGTTTATGTAACAACTTCACTGTAATTAGTTGTTGTGTTTTTTAAAAAGCCAAATATGT
CACTTTGTGTTCGACAATGAGCCAAATTTTTAGATGTTTCAGTTAGAGAAATGTTTGATTTTTTTGCATATTAAAAAGAGCACTTTGTGTTCGACAATGAGCCAAATTTTTAGATGTTTCAGTTAGAGAAATGTTTGATTTTTTTGCATATTAAAAAGAG
TATTTTCTGTAATCTGAACATAΆGGATAAGCATTGGCCCAAAAAGCTAAAATAAAGTTTTGTCATGTTCTTTTTATTTTCTGTAATCTGAACATAΆGGATAAGCATTGGCCCAAAAAGCTAAAATAAAGTTTTGTCATGTTCTTTT
Klon 0134-ContigClone 0134 contig
ANCCTCTTCTTGGGNCTAACCTAAAAAAAACCCCCCCCTGGTTTACCTGGGCTTTTTCGGAAATTTNATTNCGGNCCCCC CCTNTTAAGGGAGNCCCCNAAGCTTTGGGTNCCCAAAGCTCGGGATTCCCCTTATTTACCGGCCCGCCCCATTGTGCTGG AAAAGGTGGAASCCSCCGCCACGATGCAGATTCTTTGTGAAGACCCCTTMCGGGGAAAACCCWTCACGCTCGAGGTTGAA CCCTCGGACACTATAGAAAATGTAAAGGCCAAGATCCAGGATAAGGAAGGAATTCCTCCTGATCAGCARAGGCTGATCTT TGCTGGTAAGCAGCTGGAAGATGGCCGGACTTTGTCTGACTACAACATTCAAAAGGAGTCCACCCTTCATCTGGTGTTGA GACTTCGGGGTGGTGCTAAGAAAAGGAAGAAGAAGTCTTACACCACTCCCAAGAAGAMCAAGCATAAGAGGAAGAAGGTT AAGTTGGCTGTGCTGAAATACTATAAGGTGGATGAAAATGGCAAAATTAGCCGACTTCGTCGAGAGTGTCCTTCTGATGA ATGTGGTGCTGGAGTTTTCATGGGAAGCCACTTTGACAGGCATTACTGTGGCAAGTGTTGTCTGACTTACTGCTTTCAAC AAACCAGAAGACAAGTAGTTGTGTATGCGTTAATAAAAAGAAGGAACTCGTAAAAΆAAAAAAAAAAAANCCTCTTCTTGGGNCTAACCTAAAAAAAACCCCCCCCTGGTTTACCTGGGCTTTTTCGGAAATTTNATTNCGGNCCCCC CCTNTTAAGGGAGNCCCCNAAGCTTTGGGTNCCCAAAGCTCGGGATTCCCCTTATTTACCGGCCCGCCCCATTGTGCTGG AAAAGGTGGAASCCSCCGCCACGATGCAGATTCTTTGTGAAGACCCCTTMCGGGGAAAACCCWTCACGCTCGAGGTTGAA CCCTCGGACACTATAGAAAATGTAAAGGCCAAGATCCAGGATAAGGAAGGAATTCCTCCTGATCAGCARAGGCTGATCTT TGCTGGTAAGCAGCTGGAAGATGGCCGGACTTTGTCTGACTACAACATTCAAAAGGAGTCCACCCTTCATCTGGTGTTGA GACTTCGGGGTGGTGCTAAGAAAAGGAAGAAGAAGTCTTACACCACTCCCAAGAAGAMCAAGCATAAGAGGAAGAAGGTT AAGTTGGCTGTGCTGAAATACTATAAGGTGGATGAAAATGGCAAAATTAGCCGACTTCGTCGAGAGTGTCCTTCTGATGA ATGTGGTGCTGGAGTTTTCATGGGAAGCCACTTTGACAGGCATTACTGTGGCAAGTGTTGTCTGACTTACTGCTTTCAAC AAACCAGAAGACAAGTAGTTGTGTATGCGTTAATAAAAAGAAGGAACTCGTAAAAΆAAAAAAAAAAA
Klon 0136-BGH-SequenzClone 0136 BGH sequence
TTTTTTTTTTTTTTTTTTTTTTTTTTTGAAAATTAAATCGAGATATTTAATAGACGGKGCAAACCATAATTTCATCTGAA TATAΆATGTATGCACACGTTTCCAATGAGCTATGTATCAATTATCACAACAGCAATGAGGACCTTAGAGCTACGTTAGAA AACTGGATAGCTGGAATTAACTCTAACAATCTTATTCGCTTGCAAGAGAACACATCCATTTCAGATCCGTGTCACAAACT CCAAAAGACCAGGGGGGACAGGAGTGACAAGGGCCAGGATGGTAGACATGGGGCCAGTACACAGGTGACAGACAGAAACA CAGCTCTTGAGGGAACCAAAACTAGATGTGTTATTACAGCTAAGAACAAGGAAAAGCCTATCTATGGCTTTTAAAAAACG ACTTTTCTGCCAGTACCGTACACATCTTACAGAGTGAGACACGTCAGCTGCAGGGCTACATTTCCAGTTCGCTTCAACTG CAAACGGCCGACCCTCCACAGAGGACGCAGCAGTGGGCTTAGCGCAGCCCAGCAACTCAGTGTTGGATTTGCAAATACAG TTTATTTTACAGGAAATTCAACATTATAAAMNGGGGAACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAAAATTAAATCGAGATATTTAATAGACGGKGCAAACCATAATTTCATCTGAA TATAΆATGTATGCACACGTTTCCAATGAGCTATGTATCAATTATCACAACAGCAATGAGGACCTTAGAGCTACGTTAGAA AACTGGATAGCTGGAATTAACTCTAACAATCTTATTCGCTTGCAAGAGAACACATCCATTTCAGATCCGTGTCACAAACT CCAAAAGACCAGGGGGGACAGGAGTGACAAGGGCCAGGATGGTAGACATGGGGCCAGTACACAGGTGACAGACAGAAACA CAGCTCTTGAGGGAACCAAAACTAGATGTGTTATTACAGCTAAGAACAAGGAAAAGCCTATCTATGGCTTTTAAAAAACG ACTTTTCTGCCAGTACCGTACACATCTTACAGAGTGAGACACGTCAGCTGCAGGGCTACATTTCCAGTTCGCTTCAACTG CAAACGGCCGACCCTCCACAGAGGACGCAGCAGTGGGCTTAGCGCAGCCCAGCAACTCAGTGTTGGATTTGCAAATACAG TTTATTTTACAGGAAATTCAACATTATAAAMNGGGGAACT
Klon 0137-T7-SequenzClone 0137-T7 sequence
AGCAAGGACCCTGAAAATAAACAGCCGCTGCTTTGCGAGTCGCCTTCTTGGTTCTTCGTCCGAGTCTCCTCCGCTGTGGGAGCAAGGACCCTGAAAATAAACAGCCGCTGCTTTGCGAGTCGCCTTCTTGGTTCTTCGTCCGAGTCTCCTCCGCTGTGGG
CAGCTCAGACGCCGAAGCTCTAÄCTGCAGCCTATGAGCAGCAACGAATGCTTCAAGTGTGGACGATCTGGCCACTGGGCCCAGCTCAGACGCCGAAGCTCTAÄCTGCAGCCTATGAGCAGCAACGAATGCTTCAAGTGTGGACGATCTGGCCACTGGGCC
AGGGAGTGCCCTACTGGTGGAGGTCGGGGTCGTGGAATGAGAAGCCGCGGCAGAGGGTTCCAGTTTGTTTCCTCGTCTCTAGGGAGTGCCCTACTGGTGGAGGTCGGGGTCGTGGAATGAGAAGCCGCGGCAGAGGGTTCCAGTTTGTTTCCTCGTCTCT
CCCTGACATCTGCTACCGCTGTGGTGAGTCTGGTCATCTTGCCAAGGATTGTGATCTGCAGGAGGATGCCTGCTATAACTCCCTGACATCTGCTACCGCTGTGGTGAGTCTGGTCATCTTGCCAAGGATTGTGATCTGCAGGAGGATGCCTGCTATAACT
GCGGTAGAGGTGGCCACATTGCCAAGGACTGCAAGGAGCCCAAGAGAGAGCGAGAGCAATGCTGCTACAATTGTGGCAAGGCGGTAGAGGTGGCCACATTGCCAAGGACTGCAAGGAGCCCAAGAGAGAGCGAGAGCAATGCTGCTACAATTGTGGCAAG
CCAGGCCATCTGGCTCGTGACTGTGACCACGCGGATGAGCAGAAGTGCTATTCTTGTGGTGAATTTGGACATATTCAAAACCAGGCCATCTGGCTCGTGACTGTGACCACGCGGATGAGCAGAAGTGCTATTCTTGTGGTGAATTTGGACATATTCAAAA
AGACTGCACCAAGGTGAAGTGCTATAGGTGTGGTGAAACTGGTCATGT GCCATCAATTGCAGCAAGACAAGTGAAGTCAAGACTGCACCAAGGTGAAGTGCTATAGGTGTGGTGAAACTGGTCATGT GCCATCAATTGCAGCAAGACAAGTGAAGTCA
ACTGTTACCGCTGACTGTTACCGCTG
Klon 0137-BGH-Sequenz TTTTTTTTTTTTTTTTTTCAGTCCTCCACATTCCCCTCTTTAATATGGWATTTTGTTCTATATACATTGATGAAAATTTA AAACAAACAAAAGGAACTTTAGTCAAAACTCCCCTTCCTCCTCCAGCTTTATTGGAATAGTTTAAAATTACATTTTCTAT TTTCTAGGACTTCAGTTGCTTTTTCCATCTGACTTTTAAAAACTGATTACAGCAAATGAAACACAGTTGTCTAAGTGATA TAGTATACAAAAATAACATCTTGATTTCTGTGAAAATGCATTTCTCTGCAATTCCTGAATAGCTCCAAATTATGCTAACT CTGAGCATCGGATGTTTACTCTGGGTTTTAGATTTAGTCTTTGAAAAAATGTGTTCTACACCTTTGCCATCACCATCTGT ACGTCCAGCATCGTCACTGGGATGAAGCTGCTTCTGTGTTAGGCTTTGAGCCAGATTCCCTGGCATAGCCTTCAACACAT TTGTCTTTTCAGTTTACTCCCAACTCAACTGTATATTCTGAGGGCCTGATGCAGATGTTACTCGATGTTATTTAATAGCT ACTGAGGGTCAGACAATCCAAGGCCGNCATTATGCTAANAÄTTAAAGGNTATTTATGGAAGCTTAAATGCTTNCAGAATT GGNTTTACCTTCTACATGGGGAACACACTClone 0137 BGH sequence TTTTTTTTTTTTTTTTTTCAGTCCTCCACATTCCCCTCTTTAATATGGWATTTTGTTCTATATACATTGATGAAAATTTA AAACAAACAAAAGGAACTTTAGTCAAAACTCCCCTTCCTCCTCCAGCTTTATTGGAATAGTTTAAAATTACATTTTCTAT TTTCTAGGACTTCAGTTGCTTTTTCCATCTGACTTTTAAAAACTGATTACAGCAAATGAAACACAGTTGTCTAAGTGATA TAGTATACAAAAATAACATCTTGATTTCTGTGAAAATGCATTTCTCTGCAATTCCTGAATAGCTCCAAATTATGCTAACT CTGAGCATCGGATGTTTACTCTGGGTTTTAGATTTAGTCTTTGAAAAAATGTGTTCTACACCTTTGCCATCACCATCTGT ACGTCCAGCATCGTCACTGGGATGAAGCTGCTTCTGTGTTAGGCTTTGAGCCAGATTCCCTGGCATAGCCTTCAACACAT TTGTCTTTTCAGTTTACTCCCAACTCAACTGTATATTCTGAGGGCCTGATGCAGATGTTACTCGATGTTATTTAATAGCT ACTGAGGGTCAGACAATCCAAGGCCGNCATTATGCTAANAÄTTAAAGGNTATTTATGGAAGCTTAAATGCTTNCAGAATT GGNTTTACCTTCTACATGGGGAACACACT
Klon 0138-BGH-SequenzClone 0138 BGH sequence
TTTTTTTTTTTTTTTTTCGCATATAAATGATTCTTTATGCCCTTCCCCCATGCAGDGGGGGGCGGAGCAGGGGGAAGTCCTTTTTTTTTTTTTTTTTCGCATATAAATGATTCTTTATGCCCTTCCCCCATGCAGDGGGGGGCGGAGCAGGGGGAAGTCC
CCCGCCTCATGCAGGGAACGGGAGGTCAATGTTTAGTAGTCTTATTGCCAGATGCCCTGTGGCCATGGGGTGGGGAGTGGCCCGCCTCATGCAGGGAACGGGAGGTCAATGTTTAGTAGTCTTATTGCCAGATGCCCTGTGGCCATGGGGTGGGGAGTGG
GGGGTGTCWACAGCCCCTTGTTTTCTGGCATTGGTCTCCCTTTACCAAGTCACCATGGTGGAGCCAGGGGACTGGAGGCTGGGGTGTCWACAGCCCCTTGTTTTCTGGCATTGGTCTCCCTTTACCAAGTCACCATGGTGGAGCCAGGGGACTGGAGGCT
TAAAGCTGAGGTAAAGATGGTTCTCTCCAGGAAGAAAAGTGGTTCTCCCCGCTTGCCTGCTGGCCATCCTCAAGGTGAGTTAAAGCTGAGGTAAAGATGGTTCTCTCCAGGAAGAAAAGTGGTTCTCCCCGCTTGCCTGCTGGCCATCCTCAAGGTGAGT
ATGAGGCGGNGTCTGTGGGAANTGGAACGTCACCCAGGGGTCTCTACCCTGTGGNCAGTANGCCGGACAATGTCTCATGAGGCGGNGTCTGTGGGAANTGGAACGTCACCCAGGGGTCTCTACCCTGTGGNCAGTANGCCGGACAATGTCTC
Klon 0139-T7-SequenzClone 0139-T7 sequence
AGGGACCCCGCATCTTATTAKCAACCAGGGAGATTTCTCCATTTTCCTCTTGTACTACAGTGCGGCCTACAAATCTGGGAAGGGACCCCGCATCTTATTAKCAACCAGGGAGATTTCTCCATTTTCCTCTTGTACTACAGTGCGGCCTACAAATCTGGGA
TTTTTTTTTATTACTTCTTTTTTTAAAAAAACTACACT GGGCTCCTTTTTTGTGCTCGACTTTTCCACCTTTTTCCCTCTTTTTTTTTATTACTTCTTTTTTTAAAAAAACTACACT GGGCTCCTTTTTTGTGCTCGACTTTTCCACCTTTTTCCCTC
CTTCCTGCGCTGCTGCTTTTTTGATCTCTTCGACTAAAAATTTTTTATCCGGAGTATTTAATCGGGTCTCTTCTGTCCTCCTTCCTGCGCTGCTGCTTTTTTGATCTCTTCGACTAAAAATTTTTTATCCGGAGTATTTAATCGGGTCTCTTCTGTCCTC
CTCGCCACCCCCACCCCCTCCCTCCGGTGTGTGTGCCGCCGCCGCTGNATGCTGCTGCTGCTGCTCGCCCCGTCGTTACACTCGCCACCCCCACCCCCTCCCTCCGGTGTGTGTGCCGCCGCCGCTGNATGCTGCTGCTGCTGCTCGCCCCGTCGTTACA
CCAACCGAAGGCTCTTTGTTTCCTCTCTTGGATCTGCCGAGTTTCTTTGTTGAAGAAGCCAGCATGGGTGCCCAGNTCTCCCAACCGAAGGCTCTTTGTTTCCTCTCTTGGATCTGCCGAGTTTCTTTGTTGAAGAAGCCAGCATGGGTGCCCAGNTCTC
NAAGACCGCAGCGAAGGGAGAAGCCACCGTCGAGÄGGCCCCGNAAGACCGCAGCGAAGGGAGAAGCCACCGTCGAGÄGGCCCCG
Klon 0139-BGH-SequenzClone 0139 BGH sequence
TTTTTTTTTTTTTTTAAATCTGGGTATTGGTGTTTTTATTTTTTTACTCTTTCCTTCTTATTCAAAACGTGTAGTGTTGTTTTTTTTTTTTTTTTAAATCTGGGTATTGGTGTTTTTATTTTTTTACTCTTTCCTTCTTATTCAAAACGTGTAGTGTTGT
AAACCTGCCTCACAAAATACATCGTAATAATTTTTCTTTAAAAAAAAAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAACCTGCCTCACAAAATACATCGTAATAATTTTTCTTTAAAAAAAAAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA
AAGAAAGAAAGGACAAGAACCCTAAGCCTTTACACCCACCACCATTTTAGTGGCATATTATCTTGGCAATGTTATGCACTAAGAAAGAAAGGACAAGAACCCTAAGCCTTTACACCCACCACCATTTTAGTGGCATATTATCTTGGCAATGTTATGCACT
GCTTCAATTTCCCATAGTGGCCCCTATCACTTCATTTGTTATTCCTTTTGACCCACCCATCTCCTTCATATATGGGTATGGCTTCAATTTCCCATAGTGGCCCCTATCACTTCATTTGTTATTCCTTTTGACCCACCCATCTCCTTCATATATGGGTATG
TCTATAGATCTGACAAAGAAAGTTTACACTTTTTTAATAAAGATGCAAAGTATGCAAAAAACATTAATACTGATGCCAAA AAAAAAAAAAAAAAAGTAAAAGTAAAGAAAGAAGGCAGAGGAAGCTGTCTAACACGTCCTCGGCCTGTCGGAATGGTGGA AACAACTATCTGAGATGGGATCTGTGGGGAAGGGGGCTTAAAAAAAACANAAAACAAAAAATTTGCTGNTTAANAΆAAAT GTGAAATTTAAAANANAATNTTTAAAATNAATCCCTGGTTGTAGACANGTTNCCAAAAACCAGTACCTGGCACCACTCCATCTATAGATCTGACAAAGAAAGTTTACACTTTTTTAATAAAGATGCAAAGTATGCAAAAAACATTAATACTGATGCCAAA AAAAAAAAAAAAAAAGTAAAAGTAAAGAAAGAAGGCAGAGGAAGCTGTCTAACACGTCCTCGGCCTGTCGGAATGGTGGA AACAACTATCTGAGATGGGATCTGTGGGGAAGGGGGCTTAAAAAAAACANAAAACAAAAAATTTGCTGNTTAANAΆAAAT GTGAAATTTAAAANANAATNTTTAAAATNAATCCCTGGTTGTAGACANGTTNCCAAAAACCAGTACCTGGCACCACTCCA
ACAC
Klon 0140-Contig CTGGAAAGCTCGGTTCGGTGTCTGGGGTATGAAGGTCCTGCAAACCCGAGGCTTCGTCTCGGACTCGTCGGATAGCATGGClone 0140-Contig CTGGAAAGCTCGGTTCGGTGTCTGGGGTATGAAGGTCCTGCAAACCCGAGGCTTCGTCTCGGACTCGTCGGATAGCATGG
ATACGGGCGCTGGCTCCATCCGAGAAGCTGGTGGAGCCTTCGGAΆAACGAGAAAAGGCTGAAGAGGATCGGTACTTCCGA GAGAAGACTAAAGAACAGCTGGCTGCCCTGAGGAAACACCATGAAGATGAGATTGACCACCATTCGAAGGAGATAGAGCG TCTGCAGAAGCAAATTGAACGCCATAAGAAGAAGATCCAACAACTAAAGAATAATCATTGAATGCGCGCAGTCGGTCCCT CACAGAGTGGCCCGTATCACTCCCCACGTCTGTAGACACATGGCTTTGAATGATTACTATTTGGTCTGTGTGCTACTAAC AGATAATAAACGATCACCAGGAAAAAAAAAAAAAAAAAATACGGGCGCTGGCTCCATCCGAGAAGCTGGTGGAGCCTTCGGAΆAACGAGAAAAGGCTGAAGAGGATCGGTACTTCCGA GAGAAGACTAAAGAACAGCTGGCTGCCCTGAGGAAACACCATGAAGATGAGATTGACCACCATTCGAAGGAGATAGAGCG TCTGCAGAAGCAAATTGAACGCCATAAGAAGAAGATCCAACAACTAAAGAATAATCATTGAATGCGCGCAGTCGGTCCCT CACAGAGTGGCCCGTATCACTCCCCACGTCTGTAGACACATGGCTTTGAATGATTACTATTTGGTCTGTGTGCTACTAAC AGATAATAAACGATCACCAGGAAAAAAAAAAAAAAAAA
Klon 0141-ContigClone 0141 contig
AGGGAGGTAGAGGTGMCCGATCCTGGGGGTCGGTGTTAGGCCGTGGGAGGTTTTTCGCGAGACTGAGCCACGCGTCTATC TTCTGTCCCAGGGCCGCCGTCACCATGGGCCGCGAGTTTGGGAΆCCTGGCGCGGATACGGCACGTGATCTCCTACAGCTT GTCGCCCTTTGAGCAGCGCGCCTTCCCAAGCTATTTCAGCAAAGGCATCCCCAACGTGCTGCGCCGCACTCGCGAGCGCA TCCTGCGCGTGGCGCCGCCGTTTGTAGTGGTCTACCTGATCTACACATGGGGCAACCAGGAGTTTGAGCAGTCGAAAAGG AAGAATCCAGCCATGTATGAAAATGACAAGTAGACGGCCTGCACCTGGGTGACAGTCCCCTGCCTCTGAAAGACCCTTCT CTGGGAGAGGAATCC CMCTGTAGTCTTGAAGMCAATAAACTACTTATGGMCTTCCAAAAAAAAAAAAAAAAAAAAAAAGGGAGGTAGAGGTGMCCGATCCTGGGGGTCGGTGTTAGGCCGTGGGAGGTTTTTCGCGAGACTGAGCCACGCGTCTATC TTCTGTCCCAGGGCCGCCGTCACCATGGGCCGCGAGTTTGGGAΆCCTGGCGCGGATACGGCACGTGATCTCCTACAGCTT GTCGCCCTTTGAGCAGCGCGCCTTCCCAAGCTATTTCAGCAAAGGCATCCCCAACGTGCTGCGCCGCACTCGCGAGCGCA TCCTGCGCGTGGCGCCGCCGTTTGTAGTGGTCTACCTGATCTACACATGGGGCAACCAGGAGTTTGAGCAGTCGAAAAGG AG A A A T G AAA CCAGCC ATGTAT ATGACAAGTAGACGGCCTGCACCTGGGTGACAGTCCCCTGCCTCTGAAAGACCCTTCT CTGGGAGAGGA AT CC C C T M GTAGTCTTGAAGMCAATAAACTACTTATGGMCTTCCAAAAAAAAAAAAAAAAAAAAAA
Klon 0142-T7-SequenzClone 0142-T7 sequence
CGTTGTCTTGCTCCGGCTGCGCGCATTGTCCTCAGGGTCCTCCGACAGGGCTGCTGCGGGGCCCGGGACCCGCGCCCTAG GGACGCGCCCCCGCTGCCGGTCGGCCTGGCGCGGGGCTCTGCTAGTCTGTTGGCGAGCCCGTGCTACCGGGCTAGTCTCG CCGGGGTTTTTCCTGCGAAGTTGAGGAAGGGGAGAAGTCCCACCCGTCCGCCCAGCCCAGCCTTNCCCGGCGCGCAGCCT CGACGGGGCCGTGGCAGGCGCGACGAGGGCCGCCGACGGAGCCCTGAGAGAAGTACAAAGTGGTTNGTAACTGGGCTCGN Klon 0142-BGH-Sequenz CGTTGTCTTGCTCCGGCTGCGCG CATTGTCC CAGGGTCCTCCGACAGGGCTGCTGCGGGGCCCGGGACCCGCGCCCTAG T GG A C CGCGCCC CGCTGCCGGTCG GCCTGGCGCGGGGCTCTGCTAGTCTGTTGGCGAGCCCGTGC TA CCGGGCTAGTCTCG CCGGG GTT TT CC T G A TGCGAAGTT GGAAGGGGAGAAG CCCACCCGTCCGCCCAGCCC T A T GCC TNCCCGGCGCGCAGCCT CG A CG GG GCCGTG G AG C GC GC GACGAGGGCCGCCGACGGAGCCCTGAGAGAAGTACAAAGTGGTTNGTAACTGGGCTCGN Clone 0142 BGH sequence
TTTTTTTTTTTTTTTCAGGTTTGAAACTTTTGCTTGCCGGTGTCΆAGCAGCCAATACAGAGCCTTAATGGCCTATTTAAA CGGGGAAATATGCATGAACCAAAATCAACGAGGAACGTACACGCTGTATAGAGCCATTTTTCCACCGGGGTCTCAGATGC TCTGCAACGTACACCCTCTCAGACCCTGGCTGAAGGCTGGGGATGCTGGCTACACAAGTCAGCCAGGTTCCTCGGACATC CCAGCCAAGACAACAAAGTCGTCTCCAATCTCAAΆGCAAGAAGACAATCACATTAAGAGAATCGGGTGCGTTTGGCTTTG TGCACAGAGCGTGGCCAGCGCCCGGCGGCAGACGGCGCCTCACAGGATCACGCAGGCCGAGCAGCAGCCTCGTCGCCGTT GGGCTGTGCCGCGTAGTTCATCTGCCTCACGATCTCTGCGAATAGCTCATCCACTGAGGCTTTGTTTTTGGCCCGATGTC TCCATGAAGGGGCAGCTCCACTCCTCGGCCAGGGCCTTACCCTCGCCATAGGAGACCTCACGTTCACCCTCCAAGTCCAC CTTGTTGCCTACCAGGATCATGGGGTACGCGCTCGTACCGCTTCACGCGGATGATCTGGGCCCGCATGGGCTTGATGNTTTTTTTTTTTTTTTCAGGTTTGAAACTTTTGCTTGCCGGTGTCΆAGCAGCCAATACAGAGCCTTAATGGCCTATTTAAA CGGGGAAATATGCATGAACCAAAATCAACGAGGAACGTACACGCTGTATAGAGCCATTTTTCCACCGGGGTCTCAGATGC TCTGCAACGTACACCCTCTCAGACCCTGGCTGAAGGCTGGGGATGCTGGCTACACAAGTCAGCCAGGTTCCTCGGACATC CCAGCCAAGACAACAAAGTCGTCTCCAATCTCAAΆGCAAGAAGACAATCACATTAAGAGAATCGGGTGCGTTTGGCTTTG TGCACAGAGCGTGGCCAGCGCCCGGCGGCAGA CG G CGCCTC ACAGGATCACG C AGGCCGAGCAGCAGCCTCGTCGCCGTT GGGCT G TGCC GCG TAGTT C ATCTGCCTCAC G AT C T CTGCG AATAGCTCATCCA C TGAGGCTTTGTTTTTGGCCCGATGTC TCCATGAAGGGGCAGCTCCACTCCTCGGCCAGG G C C TTA CC CTCGCCATAGGAGACCTCACGTTCACCCTCCAAGTCCAC CTT G TT GCC T ACCAGG AT CATG G GG TA CGCGCTCGTACCGCT T CACGCG GAT GA T C TGGG C CCGCATGGGCTTGATGN
Klon 0143-T7-SequenzClone 0143-T7 sequence
TGGNAAGGGGGGACTTTTGGTATAGCATTGGAAATGTAAATGAGCTAAATACCTAATAAAAAATGGAAAAAAAAAAAAAA AAAAAAAAAAAGAAMTAGAAAAAATGTTTACTGAGGTCTTTΆTGTCTGTTGTTTTGTTTTGTTTTAGAAGTTTCTGAACG TTGTTTTAGGACTAAACTGGGAAGCCATGTGTCTTCGTTTTGGATTTTATAGATAACCTGAAGCATTATTGAGATTCCTT CAAAGTCAGTAACAACTTATTGGGTGTTTCAAGCCAGTTGTGAGTCTCTGAGTATTTGCCCAGNTTCACATTTATTGGTA AAGGTCCCTTACAAGTGGNAAGGGGGGACTTTTGGTATAGCATTGGAAATGTAAATGAGCTAAATACCTAATAAAAAATGGAAAAAAAAAAAAAA AAAAAAAAAAAGAAMTAGAAAAAATGTTTACT G AG G T T T CT ΆTGT C TGTTGTTTTGTTTTGTTTTAGAAGTTTCTGAACG TTGTTTTAGGACTAAACTGGGAAGCCATGTGTCTTCGTTTTGGATTTTATAGATAACCTGAAGCATTATTGAGATTCCTT CAAAGTCAGTAACAACTTATTGGGTGTTTCAAGCCAGTTGTGAGTCTCTGAGTATTTGCCCAGNTTCACATTTATTGGTA AAGGTCCCTTACAAG
Klon 0143-BGH-SequenzClone 0143 BGH sequence
TTTTTTTTTTTTTTTGACTCTTGCTACTTGTGAGGCTCAGCAAAAAGAATTTTTTTTTTTTTTTTGACTCTTGCTACTTGTGAGGCTCAGCAAAAAGAAT
ACTATAGATGGAATCAGTCTGTACCACTCTGTGCCATTGTCCTCTGAGTAGGTTCTCTGTGACTGTAGATTATTTTTCCA GTCTATAAAAGGTCATGGGTGCTATTGGAACTCTAGTCAGGCATCAACTTTAGGCCATCATTTCTCCATCAAAGGAAAGA GAGCTTCTACATTTCGCAGACÄTGAGCATCCTAGATTACCCATAGTGCTCAAGTGTCATTTGAGTAATTTATTGAGATAG CAAAGGCCTTTTGCATCTGATCATGCTGGGAAGAATGGAGTAAGTTTGGCATCCTTACACCTACCGAGTTGGCGACAGAC ATATTCCTCAGGAGGTTGGAATGAAATTTTTGAATGTTCAGAGGAAACCTATATACATTTAAGGTGAAATCTAAAGAATA AGAGAGAAAAACAAAACAAAACAACAAAAGAAACTCTTACCATTTGAGCACTAAGAATAGTCAAAGCAGAGGAAACAGGC TAGTGCAAAGGTCCATCTAGTACAAGAGAGNTTATGGCTTGCTCCAGGAACGGACAGAAGGCTAGCGNGGCTGGCGACTATAGATGGAATCAGTCTGTACCACTCTGTGCCATTGTCCTCTGAGTAGGTTCTCTGTGACTGTAGATTATTTTTCCA GTCTATAAAAGGTCATGGGTGCTATTGGAACTCTAGTCAGGCATCAACTTTAGGCCATCATTTCTCCATCAAAGGAAAGA GAGCTTCTACATTTCGCAGACÄTGAGCATCCTAGATTACCCATAGTGCTCAAGTGTCATTTGAGTAATTTATTGAGATAG CAAAGGCCTTTTGCATCTGATCATGCTGGGAAGAATGGAGTAAGTTTGGCATCCTTACACCTACCGAGTTGGCGACAGAC ATATTCCTCAGGAGGTTGGAATGAAATTTTTGAATGTTCAGAGGAAACCTATATACATTTAAGGTGAAATCTAAAGAATA AGAGAGAAAAACAAAACAAAACAACAAAAGAAACTCTTACCATTTGAGCACTAAGAATAGTCAAAGCAGAGGAAACAGGC TAGTGCAAAGGTCCATCTAGTACAAGAGAGNTTATGGCTTGCTCCAGGAACGGACAGAAGGCTAGCGNGGCTGGCG
Klon 0144-ContigClone 0144 contig
AGGGACAGCTCCCCTTTCTGGAAGGTGAAATTTTTGATTCTGTGAAGCCGGGACTTTCTGCTTTTGTGGATCAGCCCAAAAGGGACAGCTCCCCTTTCTGGAAGGTGAAATTTTTGATTCTGTGAAGCCGGGACTTTCTGCTTTTGTGGATCAGCCCAAA
CAGGGTGCTGAGACTGTCCAGGAGCTCTTGGAGGTGGCCAAAGACTCGATCCCCAGAAGCCACTGGGAAAGGACCCCGGTCAGGGTGCTGAGACTGTCCAGGAGCTCTTGGAGGTGGCCAAAGACTCGATCCCCAGAAGCCACTGGGAAAGGACCCCGGT
GGTTCTGAAAGCAACGGCCGGACTCCGTTTGCTGCCTGAGCAGAAAGCCCAGGCTCTGCTCTTGGAGGTAGAGGAGATCTGGTTCTGAAAGCAACGGCCGGACTCCGTTTGCTGCCTGAGCAGAAAGCCCAGGCTCTGCTCTTGGAGGTAGAGGAGATCT
TCAAGAATTCACCTTTCCTGGTCCCAGATGGCAGCGTTAGCATCATGGATGGGTCCTATGAAGGCATACTAGCCTGGGTTTCAAGAATTCACCTTTCCTGGTCCCAGATGGCAGCGTTAGCATCATGGATGGGTCCTATGAAGGCATACTAGCCTGGGTT
ACCGTGAACTTTCTAACAGGTCAGCTGCATGGTCGTGGCCAGGAGACTGTGGGGACCCTTGACCTGGGGGGTGCCTCCACACCGTGAACTTTCTAACAGGTCAGCTGCATGGTCGTGGCCAGGAGACTGTGGGGACCCTTGACCTGGGGGGTGCCTCCAC
CCAAATCACGTTTCTACCCCAGTTTGAGAAAACCCTGGAACAAACACCTAGGGGCTACCCTCACTTCCTTTGAGATGTTTCCAAATCACGTTTCTACCCCAGTTTGAGAAAACCCTGGAACAAACACCTAGGGGCTACCCTCACTTCCTTTGAGATGTTT
AACAGCACTTTTAAGCTCTATACACATAGTTACTTGGGATTTGGACTGAAAGCTGCAAGACTGGCÄACTCTGGGAGCCCTAACAGCACTTTTAAGCTCTATACACATAGTTACTTGGGATTTGGACTGAAAGCTGCAAGACTGGCÄACTCTGGGAGCCCT
GGAAGCAAAAGGGACTGATGGACATACGTTTCGAAGTGCCTGTTTACCAAGATGGTTGGAAGCAGAGTGGATCTTTGGGGGGAAGCAAAAGGGACTGATGGACATACGTTTCGAAGTGCCTGTTTACCAAGATGGTTGGAAGCAGAGTGGATCTTTGGGG
GTGTGAAATACCAGTATGGTGGTAACCAAGAAGGGGAGATGGGCTTTGAACCCTGCTATGCGGAAGTGCTGAGGGTAGTAGTGTGAAATACCAGTATGGTGGTAACCAAGAAGGGGAGATGGGCTTTGAACCCTGCTATGCGGAAGTGCTGAGGGTAGTA
CAGGGGAAACTTCACCAGCCAGAAGAÄGTCCGAGGAAGCGCCTTCTACGCTTTCTCTTACTACTACGATCGAGCCGCTGACAGGGGAAACTTCACCAGCCAGAAGAÄGTCCGAGGAAGCGCCTTCTACGCTTTCTCTTACTACTACGATCGAGCCGCTGA
CACACACTTGATCGATTATAAAAACACACACTTGATCGATTATAAAAA
Klon 0145-ContigClone 0145 contig
AAAAGGGCAGCCATGGCGCCCAGCCGGAATGGCATGATACTGAAGCCCCACTTCCACAAGGATTGGCAGCAGCGAGTGGA CACTTGGTTCAACCAGCCGGCGCGCAAGATCCGCAGGCGCAAGGCCCGGCAGGCGAAAGCGCGTCGCATCGCCCCTCGCC CCGCGTCCGGCCCCATCAGGCCCATCGTGAGGTGCCCTACAGTGAGATACCACACCAAGGTCCGGGCTGGCAGGGGCTTC AGCCTGGAGGAGCTCAGGGTGGCTGGCATCCACAAGAAAGTGGCTCGCACCATCGGCATCTCTGTGGACCCGAGGAGGCG AAACAAGTCCACGGAGTCACTGCAGGCCAACGTGCAGCGCCTGAAGGAGTACCGCTCCAAGCTCATCCTGTTCCCCAGGA AGCCTTCTGCTCCGAAGAAGGGAGACAGTTCTGCTGAAGAACTTAAGCTGGCCACCCAGCTGACAGGACCTGTGATGCCC ATCCGGAATGTGTACAAAAAGGAAAAGGCCAGAGTTATCACAGAAGAAGAGAAGAACTTTAAGGCTTTTGCCAGTCTCCG AΆTGGCCCGTGCCAATGCCCGACTCTTTGGCATCCGAGCAAΆAAGGGCGAAGGAAGCTGCAGAGCAAGATGTTGAAAAGA AAAAATAATTCTGTGTTGGAGAGCTGCAATAAATTTTCCATAAAGCAAAAAAAAAAAAAAAAAAAAAAAGGGCAGCCATGGCGCCCAGCCGGAATGGCATGATACTGAAGCCCCACTTCCACAAGGATTGGCAGCAGCGAGTGGA CACTTGGTTCAACCAGCCGGCGCGCAAGATCCGCAGGCGCAAGGCCCGGCAGGCGAAAGCGCGTCGCATCGCCCCTCGCC CCGCGTCCGGCCCCATCAGGCCCATCGTGAGGTGCCCTACAGTGAGATACCACACCAAGGTCCGGGCTGGCAGGGGCTTC AGCCTGGAGGAGCTCAGGGTGGCTGGCATCCACAAGAAAGTGGCTCGCACCATCGGCATCTCTGTGGACCCGAGGAGGCG AAACAAGTCCACGGAGTCACTGCAGGCCAACGTGCAGCGCCTGAAGGAGTACCGCTCCAAGCTCATCCTGTTCCCCAGGA AGCCTTCTGCTCCGAAGAAGGGAGACAGTTCTGCTGAAGAACTTAAGCTGGCCACCCAGCTGACAGGACCTGTGATGCCC ATCCGGAATGTGTACAAAAAGGAAAAGGCCAGAGTTATCACAGAAGAAGAGAAGAACTTTAAGGCTTTTGCCAGTCTCCG AΆTGGCCCGTGCCAATGCCCGACTCTTTGGCATCCGAGCAAΆAAGGGCGAAGGAAGCTGCAGAGCAAGATGTTGAAAAGA AAAAATAATTCTGTGTTGGAGAGCTGCAATAAATTTTCCATAAAGCAAAAAAAAAAAAAAAAAAA
Klon 0146-ContlgClone 0146-Contlg
AGGGCGAAGAGTGAGTGCCAAGGTTCATATGGGAAGGACTTTGGGGTGAGCATCTTCTCTATTTCCAGCTGGCTTTTCTG ATTTTCAGAAAGAAGACTCATCAAAGATGTCCAGCAAGACGGCAAGCACCAACAGCATAGCCCAAGCCAGGAGAACTGTG CAGCAGCTGAGATTGGAΆGCCTCCATCGAAAGAATAAAGGTCTCAAAAGCATCAGCAGACCTGATGTCATACTGTGAGGA GCATGCCCGGAGCGACCCTCTGCTGATGGGCATÄCCGACCTCAGAAAACCCGTTCAAGGATAÄGAAGACCTGCATCATCT TATAGTGGACCAGGAAGCGCCCCTTGCCTCTTAACGCAAACCACAGCAGCAACCTGAAGGGATTCCTTCAGCTTACCTGG TAACCACAGCTAGTAACTAAAACACCCTTCTCTCGGAATAATAGACCCTGAAGTCTCTCTTTTTCAAGTTGTCCTTTCTT CACCCTTTACTGATTTAATACAGAATAGCAATCTTATTTTCTATTTGATAACTATGGTATCATATTGGGTTACTGTATAA GGAAAATGGCAGGGGAGTTGTGGGAAGCTTGTCTTTACAAAATATAATTGATTAAGATATGTCAAGACCTACATTGTCTA AGCACCGGCAAATTAAAATGTCGAGAATCACTTCAGTCAAAAACCTTTATATTCTGTTCTTAATAATGTTTGTGCCAACC TATATCCCATGTAAGGGATCTGGGGAGGAGGCATGTGTCTACAACCATACCTTTTTGCACTATGGGCACTAACCACCCTG AAACTTCCTGCGGTAGCTCCCTCCCTTCAGAGTTACATCATTATCCTGACTCTGTGTAGGTAAATTTTCGTGAAATTTTT GTACAAAAAAAAAAAAAAAA AG GGCGA A GAGTGAGTGCCAAGGTTCATATGGGAAGGACTTTGGGGTGAGCATCTTCTCTA TTT CC AGC TGGCTTTTCTG A TT TTCAG A AAG AA G A C T CATCAAAGATGTCCAGCAAGACGGCAAGCACC AA CAGCATAG C CC AA GC CA GGAGAACTGTG CA GCAGC T GAGATTGG AΆ GCCTCCATCGAAAGAATAAAGGTCTCAAAAGCA T CAGC A G AC C TGATGTCATA C T G T GAGGA GCA TGCCCGGAGCGACCCTCTGCTGATGGGCATÄCCGACCTCAGAAAACCCGTTC AA GGAT AÄGAA GACC T GC AT CATCT T ATAGTGG A CCAGG AA GCGCCCCT T GCCTCTTAACGCAAACCACAGCAGC A ACCTGAAGG GATT CC TTC AGCTT A CCTGG T AACCACAGCTAGTAACTAAAACACCCTTCTCTCGGAATAATAGACCCTGAAGTCTCTCTCTTTTT CACCCTTTACTGATTTAATACAGAATAGCAATCTTATTTTCTATTTGATAACTATGGTATCATATTGGGTTACTGTATAA GGAAAATGGCAGGGGAGTTGTGGGAAGCTTGTCTTTACAAAATATAATTGATTAAGATATGTCAAGACCTACATTGTCTA AGCACCGGCAAATTAAAATGTCGAGAATCACTTCAGTCAAAAACCTTTATATTCTGTTCTTAATAATGTTTGTGCCAACC TATATCCCATGTAAGGGATCTGGGGAGGAGGCATGTGTCTACAACCATACCTTTTTGCACTATGGGCACTAACCACCCTG AAACTTCCTGCGGTAGCTCCCTCCCTTCAGAGTTACATCATTATCCTGACTCTGTGTAGGTAAATTTTCGTGAAATTTTT GTACAAAAAAAAAAAAAAAA

Claims

Patentansprüche Patent claims
1 . Apoptose-assoziierte Nukleinsäuren umfassend: (a) die in Tabelle 1 oder SEQ ID NO: 1 -225 gezeigten1 . Apoptosis-associated nucleic acids comprising: (a) those shown in Table 1 or SEQ ID NO: 1-225
Nukleinsäuren der Klone 1 - 1 24 oder die in Tabelle 2 gezeigten Nukleinsäuren der Klone 1 -124, 1 25, 127, 133, 1 34, 140, 141 , 1 44, 145, 146, dazu komplementäre Nukleinsäuren oder Fragmente davon, (b) den Sequenzen gemäß (a) im Rahmen der Degeneration des genetischen Codes entsprechende Nukleinsäuren und (c) mit den Sequenzen gemäß (a) oder/und (b) unter stringenten Bedingungen hybridisierende Nukleinsäuren.Nucleic acids of clones 1 - 1 24 or the nucleic acids of clones 1 - 124, 1 25, 127, 133, 1 34, 140, 141, 1 44, 145, 146 shown in Table 2, complementary nucleic acids or fragments thereof, (b ) nucleic acids corresponding to the sequences according to (a) in the context of the degeneration of the genetic code and (c) nucleic acids hybridizing with the sequences according to (a) and/or (b) under stringent conditions.
2. Nukleinsäuren nach Anspruch 1 , dadurch gekennzeichnet, dass sie nach Expression in einer Zelle Apoptose induzieren.2. Nucleic acids according to claim 1, characterized in that they induce apoptosis after expression in a cell.
3. Nukleinsäuren nach Anspruch 1 oder 2, dadurch gekennzeichnet, dass sie für ein Apoptose-assoziiertes Polypeptid codieren.3. Nucleic acids according to claim 1 or 2, characterized in that they code for an apoptosis-associated polypeptide.
4. Nukleinsäuren nach einem der Ansprüche 1 bis 3, dadurch gekennzeichnet, dass sie von einem eukaryontischen Organismus stammen.4. Nucleic acids according to one of claims 1 to 3, characterized in that they come from a eukaryotic organism.
5. Nukleinsäuren nach Anspruch 4, dadurch gekennzeichnet, dass sie von einem Säuger stammen.5. Nucleic acids according to claim 4, characterized in that they come from a mammal.
Nukleinsäuren nach Anspruch 5, dadurch gekennzeichnet, dass sie von humanem Ursprung sind.Nucleic acids according to claim 5, characterized in that they are of human origin.
7. Nukleinsäuren nach Anspruch 1, dadurch gekennzeichnet, dass die Teilfragmente eine Länge von mindestens 15 Nukleotiden aufweisen.7. Nucleic acids according to claim 1, characterized in that the partial fragments have a length of at least 15 nucleotides.
8. Nukleinsäuren nach einem der Ansprüche 1 bis 7, dadurch gekennzeichnet, dass sie in operativer Verknüpfung mit einer Expressionkontrolisequenz sind.8. Nucleic acids according to one of claims 1 to 7, characterized in that they are operatively linked to an expression control sequence.
9. Nukleinsäuren nach Anspruch 8, dadurch gekennzeichnet, dass die Expressionkontrolisequenz eine heterologe Expressionkontrolisequenz ist.9. Nucleic acids according to claim 8, characterized in that the expression control sequence is a heterologous expression control sequence.
10. Rekombinanter Vektor, dadurch gekennzeichnet, dass er eine Nukleinsäure nach einem der Ansprüche 1 bis 9 enthält.10. Recombinant vector, characterized in that it contains a nucleic acid according to one of claims 1 to 9.
11. Rekombinante Zelle, dadurch gekennzeichnet, dass sie mit einer Nukleinsäure nach einem der Ansprüche 1 bis 9 oder einem Vektor nach Anspruch 8 transformiert oder transfiziert ist.11. Recombinant cell, characterized in that it is transformed or transfected with a nucleic acid according to one of claims 1 to 9 or a vector according to claim 8.
12. Polypeptid, dadurch gekennzeichnet, dass es von einer Nukleinsäure nach Anspruch 1 codiert ist. 12. Polypeptide, characterized in that it is encoded by a nucleic acid according to claim 1.
3. Pharmazeutische Zusammensetzung umfassend eine Nukleinsäure nach einem der Ansprüche 1 bis 9, einen Vektor nach Anspruch 10 oder ein Polypeptid nach Anspruch 1 2, gegebenenfalls zusammen mit pharmazeutisch üblichen Träger- und Hilfsstoffen.3. Pharmaceutical composition comprising a nucleic acid according to one of claims 1 to 9, a vector according to claim 10 or a polypeptide according to claim 1 2, optionally together with pharmaceutically customary carriers and auxiliaries.
1 4. Verwendung einer Nukleinsäure nach einem der Ansprüche 1 bis 9, eines Vektors nach Anspruch 10, einer Zelle nach Anspruch 1 1 oder eines Polypeptids nach Anspruch 1 2 zur Herstellung eines diagnostischen oder therapeutischen Mittels.1 4. Use of a nucleic acid according to one of claims 1 to 9, a vector according to claim 10, a cell according to claim 1 1 or a polypeptide according to claim 1 2 for producing a diagnostic or therapeutic agent.
1 5. Verwendung nach Anspruch 1 4 zur Diagnose, Therapie oder Prävention von Apoptose-assoziierten Erkrankungen.1 5. Use according to claim 1 4 for the diagnosis, therapy or prevention of apoptosis-associated diseases.
1 6. Verwendung einer Nukleinsäure nach einem der Ansprüche 1 bis 9, eines Vektors nach Anspruch 1 0, einer Zelle nach Anspruch 1 1 oder eines Polypeptids nach Anspruch 12 zur Identifizierung von Wirksubstanzen für die Therapie oder Prävention von Apoptose- assoziierten Erkrankungen.1 6. Use of a nucleic acid according to one of claims 1 to 9, a vector according to claim 1 0, a cell according to claim 1 1 or a polypeptide according to claim 12 for identifying active substances for the therapy or prevention of apoptosis-associated diseases.
1 7. Verwendung nach Anspruch 1 6, dadurch gekennzeichnet, dass die Identifizierung in einem Hochdurchsatz-Verfahren erfolgt.1 7. Use according to claim 1 6, characterized in that the identification takes place in a high-throughput process.
1 8. Verwendung nach Anspruch 1 6 oder 1 7, dadurch gekennzeichnet, dass die Wirksubstanzen Signalwege aktivieren oder inhibieren, die durch die Expression der Nukleinsäure induziert werden.1 8. Use according to claim 1 6 or 1 7, characterized in that the active substances activate or inhibit signaling pathways which are induced by the expression of the nucleic acid.
19. Transgenes nicht-humanes Tier, (i) welches das Gen einer Nukleinsäure nach einem der Ansprüche 1 bis 9 oder das ANT-1 -Gen konstitutiv oder induzierbar überexprimiert, (ii) welches das endogene Gen einer Nukleinsäure nach einem der Ansprüche 1 bis 9 oder das ANT-1 -Gen in inaktivierter Form enthält, (iii) bei dem das endogene Gen einer Nukleinsäure nach einem der Ansprüche 1 bis 9 oder das ANT-1 -Gen vollständig oder teilweise duch ein mutiertes Gen einer Nukleinsäure nach einem der Ansprüche 1 bis 9 oder ein mutiertes ANT-1 -Gen ersetzt ist, (iv) welches eine konditionale und gewebsspezifische Über- oder Unterexpression des Gens einer Nukleinsäure nach einem der Ansprüche 1 bis 9 oder des ANT-1 -Gens aufweist oder (v) welches einen konditionalen und gewebsspezifischen Knockout des Gens einer Nukleinsäure nach einem der Ansprüche 1 bis 9 oder des ANT-1 -Gens aufweist.19. Transgenic non-human animal, (i) which constitutively or inducibly overexpresses the gene of a nucleic acid according to one of claims 1 to 9 or the ANT-1 gene, (ii) which overexpresses the endogenous gene of a nucleic acid according to one of claims 1 to 9 or the ANT-1 gene in inactivated form, (iii) in which the endogenous gene of a nucleic acid according to one of claims 1 to 9 or the ANT-1 gene is completely or partially replaced by a mutated gene of a nucleic acid according to one of claims 1 to 9 or a mutated ANT 1 gene is replaced, (iv) which has a conditional and tissue-specific over- or under-expression of the gene of a nucleic acid according to one of claims 1 to 9 or the ANT-1 gene or (v) which has a conditional and tissue-specific knockout of the gene Nucleic acid according to one of claims 1 to 9 or the ANT-1 gene.
20. Transgenes Tier nach Anspruch 19, dadurch gekennzeichnet, dass der endogene Promotor des Gens einer Nukleinsäure nach einem der Ansprüche 1 bis 9 oder des ANT-1 -Gens eine genetische Veränderung aufweist, die zu einer veränderten Expression des Gens führt.20. Transgenic animal according to claim 19, characterized in that the endogenous promoter of the gene of a nucleic acid according to one of claims 1 to 9 or of the ANT-1 gene has a genetic change which leads to an altered expression of the gene.
21 . Transgenes Tier nach Anspruch 19 oder 20, dadurch gekennzeichnet, dass es ein Nager, insbesondere eine Maus ist.21. Transgenic animal according to claim 19 or 20, characterized in that it is a rodent, in particular a mouse.
22. Verwendung eines transgenen Tiers nach einem der Ansprüche 19 bis 21 zur genetischen und/oder pharmakologischen Untersuchung von apoptotischen Prozessen, insbesondere von Krankheiten, die mit erhöhter oder verminderter Apoptose assoziiert sind.22. Use of a transgenic animal according to one of claims 19 to 21 for genetic and / or pharmacological examination of apoptotic processes, particularly diseases associated with increased or decreased apoptosis.
23. Zellkultur23. Cell culture
(i) welche das Gen einer Nukleinsäure nach einem der Ansprüche 1 bis 9 oder das ANT-1 -Gen konstitutiv oder induzierbar überexprimiert, (ii) welche das endogene Gen einer Nukleinsäure nach einem der Ansprüche 1 bis 9 oder das ANT-1 -Gen in inaktivierter Form enthält, (iii) bei der das endogene Gen einer Nukleinsäure nach einem der Ansprüche 1 bis 9 oder das ANT-1 -Gen vollständig oder teilweise duch ein mutiertes Gen einer Nukleinsäure nach einem der Ansprüche 1 bis 9 oder ein mutiertes ANT-1 -Gen ersetzt ist, (iv) welche eine konditionale und gewebsspezifische Über- oder Unterexpression des Gens einer Nukleinsäure nach einem der Ansprüche 1 bis 9 oder des ANT-1 -Gens aufweist oder (v) welche einen konditionalen und gewebsspezifischen Knockout des Gens einer Nukleinsäure nach einem der Ansprüche 1 bis 9 oder des ANT-1 -Gens aufweist.(i) which constitutively or inducibly overexpresses the gene of a nucleic acid according to one of claims 1 to 9 or the ANT-1 gene, (ii) which overexpresses the endogenous gene of a nucleic acid according to one of claims 1 to 9 or the ANT-1 gene in inactivated form, (iii) in which the endogenous gene of a nucleic acid according to one of claims 1 to 9 or the ANT-1 gene is completely or partially replaced by a mutated gene of a nucleic acid according to one of claims 1 to 9 or a mutated ANT 1 gene is replaced, (iv) which has a conditional and tissue-specific over- or under-expression of the gene of a nucleic acid according to one of claims 1 to 9 or the ANT-1 gene or (v) which has a conditional and tissue-specific knockout of the gene Nucleic acid according to one of claims 1 to 9 or the ANT-1 gene.
24. Zellkultur nach Anspruch 23, dadurch gekennzeichnet, dass sie aus humanen Zellen besteht.24. Cell culture according to claim 23, characterized in that it consists of human cells.
25. Verwendung einer Zellkultur nach Anspruch 23 oder 24 zur genetischen und/oder pharmakologischen Untersuchung von apoptotischen Prozessen, insbesondere von Krankheiten, die mit erhöhter oder verminderter Apoptose assoziiert sind. 25. Use of a cell culture according to claim 23 or 24 for the genetic and/or pharmacological examination of apoptotic processes, in particular diseases that are associated with increased or decreased apoptosis.
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