EP1349929A2

EP1349929A2 - Apoptosis-inducing dna sequences

Info

Publication number: EP1349929A2
Application number: EP01954041A
Authority: EP
Inventors: Stefan Grimm; Nicole SCHÖNFELD; Erik Braziulis; Ursula Cramer; Andreas Gewies; Frank Voss; Thomas Mund; Timur Albayrak; Hendrik Gille; Matthias Klein; Manuel Bauer
Original assignee: Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Current assignee: Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Priority date: 2000-07-14
Filing date: 2001-07-13
Publication date: 2003-10-08
Also published as: AU2001276406A1; WO2002006479A3; WO2002006479A2

Abstract

The invention relates to novel apoptosis-associated and especially apoptosis-inducing nucleic acid sequences, polypeptides which are coded by said sequences, and the use of the same for preparing diagnostic and therapeutic agents. The invention also relates to cell systems, transgenic animals and the use thereof for genetic and/or pharmacological research into apoptosis-associated illnesses.

Description

Apoptosis-inducing DNA sequences

description

The invention relates to new apoptosis-associated and in particular apoptosis-inducing nucleic acid sequences, polypeptides encoded thereof and their use for the provision of diagnostic and therapeutic agents. In addition, the invention relates to transgenic cell systems and animals and their use for the genetic and / or pharmacological investigation of apoptosis-associated diseases.

Apoptosis is the genetically encoded suicide program that is induced in eukaryotic cells under certain physiological or pathological conditions. The induction of apoptosis must be regulated extremely precisely because hyperactivity can lead to degenerative diseases. On the other hand, reduced apoptosis induction can contribute to tumor progression.

Various low-molecular inducers of apoptosis have already been described. An important class is tumor cytostatics. How these cytostatics or other substances can induce apoptosis is, however, in most cases unknown.

The identification of apoptosis-inducing genes or other dominant genes with a non-selectable activity is problematic since stable recombinant expression of such genes in a target cell is either not possible or is very difficult. It is therefore necessary to use special screening methods to identify such genes. Various in vitro methods have already been developed for this (King et al., Science 277 (1 997), 973-974 and Lustig et al., Meth. Enzymol. 283 (1 997), 83-99). From other working groups transgenic mice containing multiple transgenes, the functions of which are determined by examining the phenotype (Simonet et al., Cell 89 (1997), 309-319 and Smith et al., Nat. Genet. 16 (1997), 28-36 ). A disadvantage of the in vitro methods is that the results obtained do not readily correlate with complex regulated cell biological effects. In turn, studies on transgenic animals are very complex and tedious.

Grimm and Leder (J. Exp. Med. 185 (1997), 1 137-1 142) describe a method for identifying and isolating dominant apoptosis-inducing nucleic acid sequences. Small plasmid pools corresponding to 20 clones from normalized cDNA expression libraries are transiently introduced into the human kidney cell line 293. The apoptosis-inducing activity of a nucleic acid sequence is determined manually by microscopic inspection for morphological features characteristic of apoptosis. The apoptosis-inducing adenine nucleotide translocase 1 (ANT-1) gene was identified using this method. The ANT-1 gene is considered to be the cause of the degenerative cardiac disease dilatory cardiomyopathy (DCM) (PCT / EP00 / 08812).

In the described method 293 plasmid pools of 20 clones are used for transfection into the kidney cell line. The transfection of plasmid pools, however, can only achieve a low sensitivity for the screening method, since those expression plasmids which contain apoptosis-inducing genes are present in each case in a low ratio to the expression plasmids which do not carry apoptosis-inducing genes. In order to avoid this problem, aliquots which contained only one clone statistically were used instead of plasmid pools in the screening method used for transfection. In addition, a particularly effective way of purifying plasmid DNA was used (Neudecker and Grimm, Biotechniques 28 (2000), 107-109). This procedure enables Reduction of lipopolysaccharides by a factor of 900 compared to previously published methods and thus enables for the first time the purification of plasmid DNA for high throughput in the "96 well" format. The use of specially purified DNA for transfection in combination with the use of individual clones instead of plasmid pools enabled a particularly effective and sensitive screening process to be carried out, which made it possible to determine a large number of apoptosis-inducing sequences.

The present invention relates to new apoptosis-associated and in particular apoptosis-inducing nucleic acids comprising: (a) the nucleic acids of clones 1 - 1 6 shown in Table 1, in Table 2 or in SEQ ID NO: 1 - 225, and nucleic acids complementary thereto or fragments thereof, (b) nucleic acids corresponding to the sequences according to (a) in the context of the degeneration of the genetic code and (c) nucleic acids hybridizing with the sequences according to (a) or / and (b) under stringent conditions.

The nucleic acids according to the invention are apoptosis-associated nucleic acids, ie nucleic acids which are associated with the occurrence of apoptotic processes in a cell, in particular in a mammalian cell. The nucleic acids are preferably apoptosis-inducing nucleic acids, ie nucleic acids which can induce and / or promote apoptotic processes. The nucleic acids according to the invention are particularly preferably dominant apoptosis-inducing nucleic acids which are capable of inducing apoptosis when expressed in a cell and which produce the characteristics characteristic of apoptosis, such as DNA fragmentation, morphological peculiarities etc. The nucleic acids can be in double-stranded or single-stranded form, for example as DNA or RNA. The isolated nucleic acids can develop their cellular effect by expression, in particular by overexpression in cells. This makes them inducible and defines their use as a therapeutic agent.

In addition to the nucleic acids or partial fragments thereof shown in Table 1 or the corresponding sequence protocols, with a length of preferably at least 15, particularly preferably at least 20 and most preferably at least 25 nucleotides, variants of these sequences are also covered by the present invention. In addition to the nucleic acids which correspond to the sequences according to (a) in the context of the degeneration of the genetic code and which code for a polypeptide with the same amino acid sequence, nucleic acids are also recorded which are stringent with the sequences according to (a) or / and (b) Hybridize conditions. In the context of the present application, hybridization under stringent conditions means that after pre-hybridization and hybridization under suitable conditions and washing in 1 × SSC and 0.1% SDS at 55 ° C., preferably at 62 ° C. and particularly preferably at 68 ° C. and in particular a hybridization signal is also found in 0.2 x SSC and 0.1% SDS at 55 ° C, preferably at 62 ° C and particularly preferably at 68 ° C (see also Sambrook et al., Molecular Cloning. A Laboratory Manual (1 989), Cold Spring Harbor Laboratory Press, 1 .101 -1 .104).

The apoptosis-associated nucleic acids according to the invention preferably code for an apoptosis-associated polypeptide or a functional fragment thereof. The nucleic acids can originate from any organism, eukaryotic organisms such as nematodes, for example C. elegans, arthropods such as Drosophila, Cordata and vertebrates, for example mammals, being preferred. Sequences from mammals, for example from the mouse or from humans, are particularly preferred, these sequences optionally being able to be modified by known molecular biological techniques, such as site-specific mutagenesis, PCR, restriction cleavage and ligation. The nucleic acids according to the invention are preferably operatively linked to an expression control sequence so that they can be transcribed and, if necessary, translated in a suitable host cell. Expression control sequences usually comprise a promoter and optionally regulatory sequences such as operators or enhancers. Translation initiation sequences may also be present. Suitable expression control sequences for prokaryotic or eukaryotic host cells are known to the person skilled in the art (see, for example, Sambrook et al., Supra).

Another object of the invention is a recombinant vector which contains a nucleic acid according to the invention, preferably in operative linkage with an expression control sequence. The recombinant vector can also contain common elements such as an origin of replication and a selection marker gene. Examples of suitable recombinant vectors, e.g. Plasmids, cosmids, phages, viruses etc. are known to the person skilled in the art (see e.g. Sambrook et al., Supra).

The invention further relates to recombinant cells which can be transformed or transfected with a nucleic acid or a vector according to the invention. The transformation or transfection can be carried out according to known methods, e.g. by calcium phosphate coprecipitation, lipofection, electroporation, particle bombardment or viral infection. The cell according to the invention can contain the recombinant nucleic acid in extrachromosomal or chromosomally integrated form.

Another object of the invention is apoptosis-associated polypeptides which are encoded by a nucleic acid according to the invention. Apoptosis-associated polypeptides can be obtained by expression of the apoptosis-associated nucleic acids according to the invention, by chemical synthesis or by combinations of both methods. Another object of the invention is a pharmaceutical composition which contains a nucleic acid according to the invention, a vector according to the invention or a polypeptide according to the invention optionally together with pharmaceutically customary excipients and auxiliaries. The nucleic acids, vectors, cells and polypeptides described above can be used to produce a diagnostic or therapeutic agent, in particular an agent for the diagnosis, therapy or prevention of apoptosis-associated diseases. Apoptosis-associated diseases can be characterized on the one hand by an abnormally reduced apoptosis and thus by hyperproiiferation, for example tumor diseases, autoimmune diseases and viral infections (Thompson, Science 267 (1995), 1456-1462). On the other hand, apoptosis-associated diseases can also be characterized by abnormally increased apoptosis and thus by degenerative symptoms, such as Alzheimer's disease, Huntington's disease, Parkinson's disease, reperfusion damage, stroke and alcohol damage to the liver (Thompson (1995), supra ).

The diagnostic application comprises a qualitative and / or quantitative detection of the apoptosis-associated nucleic acid, for example in the form of a transcript, or the polypeptide encoded thereby in a sample, in particular a sample, which was taken from a diseased organism, for example a patient. The detection can be carried out in the usual way, for example by nucleic acid hybridization or amplification reactions such as PCR or by protein detection using antibodies. Numerous techniques for this are known to the person skilled in the art. The detection can also be done by using the isolated genes on a DNA chip. This allows several, eg all genes to be examined simultaneously in one experiment. The therapeutic or preventive application comprises the administration of an active substance to a diseased organism in a sufficient dosage to alleviate or cure the apoptosis-associated disease or to prevent the onset of an apoptosis-associated disease. In one embodiment of the invention, an apoptosis-associated nucleic acid is administered on a gene therapy vector, for example an adenovirus, a retrovirus, an adeno-associated virus, etc., in order to bring about increased expression of the apoptosis-associated nucleic acid in a diseased target cell. Alternatively, an antisense nucleic acid can also be administered, for example on a gene therapy vector or also directly, provided that the expression of the apoptosis-associated nucleic acid is to be reduced. In a further embodiment of the invention, apoptosis-associated polypeptides or modulators of the activity of such apoptosis-associated polypeptides, for example activators or inhibitors, can be administered. The active substances are administered by known methods, for example in gene therapy (Anderson, Nature 392 (1998), 25-30) or protein therapy (Schwarze et al., Science 285 (1999), 1569-1572).

Finally, the nucleic acids, vectors, cells and polypeptides according to the invention can also be used to identify new active substances for the therapy or prevention of apoptosis-associated diseases. The use in known cellular or molecular screening assays is conceivable here, in a high throughput format. The invention naturally also relates to the active substances identified by the use of such screening methods or substances derived therefrom. The active substances identified by the screen are able to activate or inhibit signaling pathways which are induced by the expression of the nucleic acids. The present invention further provides transgenic non-human animals which (i) constitutively or inducibly overexpress the gene of a nucleic acid according to the invention or the ANT-1 gene, (ii) the endogenous gene of a nucleic acid according to the invention or the ANT-1 gene in inactivated form Contain form, (iii) contain the endogenous gene of a nucleic acid according to the invention or the ANT-1 gene completely or partially replaced by a mutated gene of a nucleic acid according to the invention or a mutated ANT-1 gene, (iv) conditional and tissue-specific overexpression or underexpression of the gene of a nucleic acid according to the invention or of the ANT-1 gene or (v) have a conditional and tissue-specific knock-out of the gene of a nucleic acid according to the invention or of the ANT-1 gene.

The transgenic animal can preferably additionally contain an exogenous gene of a nucleic acid according to the invention or an exogenous ANT-1 gene under the control of a promoter which allows overexpression. Alternatively, the endogenous gene of a nucleic acid according to the invention or the endogenous ANT-1 gene can be overexpressed by activation or / and exchange of the own promoter. The endogenous promoter of the gene of a nucleic acid according to the invention or of the ANT-1 gene preferably has a genetic change which leads to a changed expression of the gene. The genetic modification of the endogenous promoter includes a mutation of individual bases as well as deletion and insertion mutations.

A first embodiment relates to a transgenic animal which constitutively or inducibly overexpresses the gene of a nucleic acid according to the invention or the ANT-1 gene. The introduced gene of a nucleic acid according to the invention or the introduced ANT-1 gene can optionally have additional mutations. A second embodiment relates to a transgenic animal which contains the endogenous gene of a nucleic acid according to the invention or the endogenous ANT-1 gene in inactivated form. The inactivation of the gene of a nucleic acid of the invention or of the ANT-1 gene is preferably effected by introducing a knock-out mutation ^"by homologous recombination or by introducing an antisense construct or an RNAi Kontrukts.

A third embodiment relates to a transgenic animal in which the endogenous gene of a nucleic acid according to the invention or the endogenous

ANT-1 gene is completely or partially replaced by a mutated gene of a nucleic acid of the invention or a mutated ANT-1 gene.

A fourth embodiment relates to a transgenic animal which has a conditional and tissue-specific overexpression or underexpression of the gene of a nucleic acid according to the invention or of the ANT-1 gene.

In a fifth embodiment, the transgenic animal has a conditional and tissue-specific knock-out of the gene of a nucleic acid according to the invention or of the ANT-1 gene.

The transgenic animal is preferably a mammal, such as a rodent, for example a mouse. Mice have numerous advantages over other animals. They are easy to hold and their physiology is considered a model system for that of humans. The production of such gene-manipulated animals is well known to the person skilled in the art and is carried out by customary methods (Hogan, B., Beddington, R., Costantini, F. and Lacy, E. (1994), Manipulating the Mouse-Embryo; A Laboratory Manual, 2nd ed., Could Spring Harbor Laboratory, Cold Spring Harbor, NY). The invention further relates to the use of such a transgenic animal for the genetic and / or pharmacological examination of diseases which are associated with excessive or reduced or no expression of a gene of a nucleic acid according to the invention or of an ANT-1 gene.

The transgenic animals according to the invention can serve as a model for the diseases associated with the gene of a nucleic acid or ANT-1 gene according to the invention in humans or also in farm animals. For example, the effects of drugs or gene therapies on the course of the disease can be determined. The animals can also be useful for diagnosis or early detection of a disease.

For example, a transgenic animal according to the invention which contains the ANT-1 gene can serve as a model for the degenerative heart disease dilatoric cardiomyopathy (DCM). This degenerative heart disease is associated with excessive apoptosis in a patient's heart cells. A first indication that the apoptosis inducer ANT-1 plays an important role in the induction of apoptosis in DCM was the observation that the expression pattern of the ANT-1 isoforms in the patient developed very early in the course of DCM Heart shifts. There is an increased expression of ANT-1 mRNA and ANT-1 protein (PCT / EP00 / 0881 2). For this purpose, the ANT-1 gene can be expressed in transgenic mice under the control of the heart-specific αr-myosin heavy chain promoter (Subramaniam, A. (1 991), J. Biol. Chem. 266 (36), pages 24613-24620) become. This promoter is well characterized and is only activated at the time of birth. The expression construct can be produced, for example, by inserting the ANT-1 gene into the Sall restriction site of the third non-coding exon of the 5.5 kB promoter. The hearts of the transgenic animals according to the invention thus produced should contain some of the cellular ones which are specific to DCM Changes such as fibrinization, apoptosis and hypertrophy, or functional disorders such as left ventricular pressure, end diastolic pressure, contractility, left ventricular ejection fraction and left ventricular filling pressure.

Alternatively or additionally, cell culture systems, in particular human cell culture systems, can also be used for the applications which are described for the transgenic animal.

The invention is further illustrated by the following example.

The sequence listing contains the sequences SEQ ID No. 1-225, which include the T7 sequences, BGH sequences and internal primer sequences of the identified apoptosis-inducing genes of clones 1-124 listed in Table 1. Table 2 shows the sequences of clones 1-124 and 125, 127, 133, 134, 140, 141, 144, 145 and 146.

Example isolation of apoptosis-inducing genes

1. General

Apoptosis-inducing genes were found by a genetic screen in the human cell line HEK 293T (Grimm and Leder (1997), supra), which is based on the iterative transfection of small expression plasmid pools from a normalized gene library and the subsequent microscopic determination of the programmed cell death the phenotype of the apoptotic cells. The transfection of individual clones from a positive plasmid pool then allows the apoptosis-inducing gene to be determined. This screen was done in a 96-well format. Furthermore, a particularly effective way of purifying the plasmid DNA was used (Neudecker and Grimm, Biotechniques 28 (2000), 107-109).

2. Experimental protocols

2.1 Cell culture and transfections

Human HEK 293T cells were supplemented in DMEM with 5% fetal calf serum (Sigma, Deisenhofen, Germany) in a humidified 5% CO ₂ atmosphere. For transfections, the cells were placed in 24-well plates and treated with 2 μg plasmid DNA using the calcium phosphate coprecipitation method as described by Roussel et al. (Mol. Cell. Biol. 4 (1984), 1999-2009). For this, 25 μ \ DNA solution with 25 μ \ 2 x HBS buffer pH 6.9 (274 mM NaCl, 10 mM KCI, 40 mM Hepes, 1, 4 mM Na ₂ HPO ₄ ) at 4 ° C in a 96- Perforated plate mixed with a 1 2-channel pipetting machine (Eppendorf, Hamburg, Germany). After adding 20 μl of a 0.25 M CaCl ₂ solution (4 ° C.) and mixing, 38 μl were added to the cells after incubation for 25 min at room temperature.

2.2 Generation of a normalized library and cDNA Screeninα The normalization and construction of a kidney cDNA library was carried out as described by Grimm and Leder (J. Exp. Meth. 185 (1997), 1 137-1 142) and Sasaki et al. (Nucleic Acids Res. 22 (1994), 987-992).

Kidney mRNA from 10 week old CD1 mice was normalized by association of abundant mRNA species with antisense cDNA molecules covalently coupled to latex beads and subsequent separation by centrifugation. After two rounds of hybridization, 200 ng (from originally 2 μg) of mRNA were obtained and used to prepare a cDNA library using a cDNA synthesis kit (Gibco BRL, Gaithersburg, MD) used. After ligating a BstXI adapter (Invitrogen, San Diego, CA) and cleaving with Notl, the cDNA molecules were inserted into a modified pcDNA3 vector (Invitrogen) under the control of the cytomegalovirus (CMV) promoter in which the neomycin resistance gene had been deleted. The DNA was introduced by electroporation into E. coli SURE cells (Stratagene, Corp. La Jolla, CA), which were then immediately frozen.

By plating aliquots of the transformation mixture on agar, it was found that the library contained approximately 2.5x10 ⁵ clones. Aliquots containing statistically single clones were inoculated in holes of 96-hole blocks (Qiagen, Hilden, Germany) in 900 μ \ LB medium and cultured for 30 hours with shaking at 300 rpm. The remaining DNA was used to transform bacteria for large-scale plasmid isolation and to sequence the inserted DNA. On the basis of the DNA sequence, a sequence comparison with commercial sequence databases was carried out with the aid of the "Blast" computer program

2.3 Determination of the Apoptosis-Inducing Nucleic Acids The apoptosis-inducing activity of the transfected nucleic acids was carried out by microscopic determination of the cell phenotype. In the case of apoptotic cells, the optical density of the cells increases because the cytoplasmic nucleus volume ratio decreases and as a result of the breakdown of the cytoskeleton, bubbles form in the cytoplasmic membrane.

2.4 Plasmid isolation

96-hole blocks with bacteria were centrifuged for 5 min at 3000 g (Sigma centrifuges, Osterode am Harz, Germany). The supernatant was decanted and the blocks were inverted for 2-3 minutes. Then 1 70 μ \ buffer P1 (50 mM Tris-HCl / 1 OmM EDTA pH 8.0) was added and the bacterial pellets were resuspended by complete vortex treatment for 10 to 20 min. After adding 1 70 μ \ buffer P2 (200 mM NaOH, 1% SDS), the block was sealed with film, mixed by inverting and incubated for 5 min at room temperature. The lysis was terminated by adding 170 μl of 4 ° C. cold buffer P3 (3 M potassium acetate pH 5.5). Then 10 μ RNaseA solution (1.7 mg / ml) were added, incubated for 5 min at room temperature and then at -20 ° C. and centrifuged again for 10 min at 6000 rpm. The supernatant was decanted into new blocks and 100 μl buffer P4 (2.5% SDS in isopropanol) was added. The block was vortexed for 5 min and incubated first for 15 min at 4 ° C and then for 15 min at -20 ° C.

The supernatant after centrifugation for 10 min at 6000 rpm was placed in 96-hole polyoxymethylene microtiter blocks. 1 50 ul silica suspension was added and incubated for 20 min at room temperature. The plates were centrifuged for 5 minutes at 6000 rpm. The supernatant was carefully decanted and 400 ul acetone (-20 ° C) was added. The plates were again vortexed (30 sec) and centrifuged for 3 min at 6000 rpm. This acetone washing process was repeated once. The plates were first dried at room temperature for 5 minutes and then for 5 minutes in a vacuum chamber. The pellets were resuspended in 75 ul water (60 ° C) and centrifuged at 6000 rpm and 4 ° C for 10 min. The supernatant was stored in a 96-well microtiter plate at -20 ° C.

Results

Clone 1 is composed of the following sequences: T7, BGH sequence, internal primer on the left and internal primer on the right (Seq. 1-4). The overall sequence is given under Contig (Seq. 5). Clone 1 shows homology with the following ESTs (expressed sequence tags): uc81 e12.y1 Sugano mouse kidney mkia Mus musculus cDNA clone (Genbank Acc. No AA986577.1 | AA986577) with an identity of 99%; mq28c01.r1 Barstead MPLRB1 Mus musculus cDNA clone (Genbank Acc. No AA1 37597. 1 J AA1 37597) with an identity of 100%; vc94d05.r1 Barstead MPLRB1 Mus musculus cDNA clone (Genbank Acc. No AA388338.1 | AA388338) with an identity of 99%; vg82f08.r1 Barstead MPLRB1 Mus musculus cDNA clone (Genbank Acc. No AI098009.1 | AI098009) with an identity of 99%; uc81 f01 .x1 Sugano mouse kidney mkia Mus musculus cDNA clone (Genbank Acc. No AA986094.1 | AA986094) with an identity of 100%.

Clone 2 is composed of the T7 and BGH sequences. The overall sequence is given under Contig. Clone 2 shows homology with the following database entries: Homo sapiens cDNA FLJ20625 fis, clone KAT04008 (DNA Data Bank of Japan (DDBJ) Acc. No AK000632.1 and AK000632) with an identity of 91%; mf71 g09.r1 Soares mouse embryo NbME1 3.5 14.5 Mus musculus cDNA clone (Genbank Acc. No W89554.1 J W89554) with an identity of 99%; G0103H 1 2-3 Mouse E7.5 Embryonic Portion cDNA Library Mus musculus cDNA clone (Genbank Acc. No AW536394.1 j AW536394) with an identity of 99%.

Clone 3 is composed of the T7 and BGH sequences. The overall sequence is given under Contig. Clone 3 shows homology with EMBL Acc. No X78936 | MMPHRPR: M. musculus mRNA for parathyroid hormone / parathyroid hormone related peptide receptor with an identity of 94%.

Clone 4 is composed of the T7 and BGH sequences. The overall sequence is given under Contig. Clone 4 shows homology with Mus musculus mRNA for calcium Channel gamma 5 subunit (CACNG5 gene) (EMBL Acc. No AJ272046.1 J MMU272046) with an identity of 97%.

Clone 5 shows homology with RefSeq database Acc. No NM_00821 8.1: Muscle hemoglobin alpha, adult chain 1 (Hba-a1), mRNA with one Length of 564bp and an identity of 97% as well as Genbank Acc. No L75940.1 1 MUSALGL: Mus muscle alpha-globin mRNA, complete cds with an identity of 98%.

Clone 6 is composed of T7 or R2 and BGH or 4SP6 sequence. The overall sequence is given under Contig. Clone 6 shows homology with the following database entries: Mus musculus domesticus mitochondrial carrier homolog 1 isoform a mRNA, complete cds; nuclear gene for mitochondrial (Genbank Acc. No AF176007.2 J AF176007) with an identity of 99%; Homo sapiens CGI-64 protein mRNA, complete cds (Genbank Acc. No AF151822.1 | AF151822) with an identity of 90%; Homo sapiens presenilin-associated protein mRNA, complete cds (Genbank Acc. No AF189289.1 | AF189289) with an identity of 89%.

Clone 7 shows homology to Genbank Acc. No AF151893.1 | AF151893: Homo sapiens CGI-135 protein mRNA, complete cds with an identity of 86%.

Clone 8 shows homology to RefSeq Acc. No NM_012504.1: Rattus norvegicus ATPase, Na + K -t-transporting, alpha 1 polypeptide (Atp1 a1), mRNA with an identity of 94%.

Clone 9 shows homology to the following database entries: Mus musculus solute carrier family 27 (fatty acid transporter), member 2 (Slc27a2), mRNA (RefSeq Acc. No NM_01 1978.1) with an identity of 99%; Mus muscle fatty acid transport protein 2 mRNA, complete cds (Genbank Acc. No AF072757.1 J AF072757) with an identity of 99%; Mus muscle mRNA for very-long-chain acyl-CoA synthetase (VLACS) (EMBL Acc. No AJ223958.1 J MMAJ3958) with an identity of 99%.

Clone 10 is composed of the T7 and BGH sequences. The overall sequence is given under Contig. Clone 10 shows homology EMBL Acc. No AL080066.1 J HSM800567: Homo sapiens mRNA; cDNA DKFZp564J 142 (from clone DKFZp564J142) with an identity of 89-95% as well as to DDBJ Acc. No AK001993.1 | AK001993: Homo sapiens cDNA FLJ1 1 131 fis, clone PLACE 1006325, highly similar to Homo sapiens mRNA; cDNA DKFZp564J142 with an identity of 89-95%.

Clone 1 1 shows homology to the following database entries: RefSeq Acc. No NM_009984.1: Mus muscle cathepsin L (Ctsl), mRNA with an identity of 96%; EMBL Acc. No X06086.1 1 MMMEPR: Mouse mRNA for major excreted protein (MEP) with an identity of 96%; Genbank Acc. No J02583.1 j MUSCPR: with an identity of 96%.

Clone 12 is composed of the T7 and BGH sequences. The overall sequence is given under Contig. Clone 12 shows homology to the following database entries: EMBL Acc. No AL137721.1 | HSM802233: Homo sapiens mRNA; cDNA DKFZp761 H221 with an identity of 89%; Genbank Acc. No L34839.1 j HUMTUM: Homo sapiens over-expressed breast tumor protein mRNA with an identity of 89%; Genbank Acc. No AF216754.1 J AF216754: Homo sapiens over-expressed breast tumor protein (OBTP) mRNA, complete cds with an identity of 89%; Genbank Acc. No U16813 j HSU16813: Human Bak-3 pseudogenic, complete cds with an identity of 88%. Clone 12 shows homology with the following ESTs: DDBJ Acc. No C88895 | C88895: Mus musculus early blastocyst cDNA, clone 01 B00051 IK19 with an identity of 97% and Genbank Acc. No AA268719 | AA268719: Soares mouse NML Mus musculus cDNA clone 747619 5 'with an identity of 97%:

Clone 13 is composed of the T7 and BGH sequences. The overall sequence is given under Contig. Clone 13 shows homology to Genbank Acc. No AF151807.1 JAF151807: Homo sapiens CGI-49 protein mRNA, complete cds with an identity of 87-90%. Clone 14 shows homology to Genbank Acc. No AF080469] AF080469: Mus muscle putative glycogen storage disease type 1b protein mRNA, complete cds with an identity of 94%.

Clone 15 shows an identity to Genbank Acc. No U31241 | CGU31241: Cricetulus griseus integral membrane protein CII-3 mRNA, nuclear gene encoding mitochondrial protein, complete cds with an identity of 85% and to Genbank Acc. No S74803JS74803: CII-3 succinate-ubiquinone oxidoreductase complex II membrane-intrinsic subunit [cattle, heart, mRNA with an identity of 83%.

Clone 16 is composed of T7 or 4SP6 and BGH sequences. The overall sequence is given under Contig. Clone 16 shows homology to Genbank Acc. No AF116911.1 | AF116911: Mus musculus thymic dendritic cell-derived factor 1 mRNA, complete cds with an identity of 96% and for RefSeq Acc. No NM_004872.1: Homo sapiens mouse tropomyosin homolog (HSPC001) mRNA with an identity of 89-92%.

Clone 17 shows an identity to Genbank Acc. No AF056031 JAF056031: Rattus norvegicus kynurenine 3-hydroxylase mRNA, complete cds with an identity of 90% and EMBL Acc. No Y1.3153JHSKYNU3MO: Homo sapiens mRNA for kynurenine 3-monooxygenase with an identity of 82%.

Clone 18 is composed of the T7 and BGH sequences. The overall sequence is given under Contig. Clone 18 shows homology to Genbank Acc. No AW109849.11 AW109849: MT2475 mouse liver, dioxin treated Mus musculus cDNA clone MT24753 'with an identity of 94%; Genbank Acc. No AA277327.1 | AA277327: va81 e12.r1 Soares mouse NML Mus musculus cDNA clone IMAGE: 7378065 'similar to WP: C02F5.3 0.CE00039 GTP-BINDING PROTEIN with an identity of 93%; Genbank Acc. No AI 646762.1 | AI 646762: u b65e01. x 1 Soares_mammary_gland_NMLMG Mus musculus cDNA clone IMAGE: 13826163 'similarto WP: C41D11.5 CE08662 ENDONUCLEASE with an identity of 94%.

Clone 19 shows homology to DDBJ Acc. No AB005451 | AB005451: Mus musculus mRNA for RST, complete cds with an identity of 92%.

Clone 20 shows homology to Genbank Acc. No AA109018 | AA109018; mp37f03.r1 Barstead MPLRB1 Mus musculus cDNA clone 571421 5 'similar to SW: ACY2_HUMAN P45381 ASPARTOACYLASE with an identity of 96%.

Clone 21 shows homology to the following database entries: Genbank Acc. No M12673JRATGNPAS: RATGNPAS Rat guanine nucleotide-binding protein G-s, alpha subunit mRNA complete cds with an identity of 87% and EMBL Acc. No Y00703 | MMGTPAMU: Mouse uncoupled S49 cells mRNA for stimulatory GTP-binding protein alpha subunit with an identity of 87%; Genbank Acc. No M17525JRATBPGTPD: RATBPGTPD Rat GTP-binding protein (G-alpha-8) mRNA, complete cds with an identity of 87%; Genbank Acc. No AF116268 | AF116268: Mus musculus G-protein XLAS (Xlas) mRNA, altematively spliced, complete cds with an identity of 94%.

Clone 22 shows homology to the following database entries: Genbank Acc. No AF061026 | AF061026: Mus musculus leucine zipper-EF-hand containing transmembrane protein 1 (Letml) mRNA, complete cds with an identity of 93-95%.

Clone 23 shows homology to the following database entries: Genbank Acc. No AA086895JAA086895: mk19c02.r1 Soares mouse p3NMF19.5 Mus musculus cDNA clone 4933465 'similar to WP: F17C11.8 CE05655 and to Genbank Acc. No AA881548 | AA881548: vx20b03.r1 Soares 2NbMT Mus musculus cDNA clone 1264973 5 'with an identity of 88% and to Genbank Acc. No AA073042 J AA073042: mm78b04.r1 Stratagene mouse embryonic carcinomaRA (# 937318) Mus musculus cDNA clone 53451 1 5 'similar to WP: F17C1 1.8 with an identity of 89%;

Clone 24 shows homology to Genbank Acc. No U22465 | MMU22465: Mus musculus Na / Pi-cotransporter (NaPi-7) mRNA, complete cds with an identity of 93% and to Genbank Acc. No L33878 | MUSSPT: Mus musculus renal sodium phosphate (Na + / Pi) transporter mRNA complete cds with an identity of 93%.

Clone 25 shows homology to Genbank Acc. No AF049882: Rattus norvegicus metastasis suppressor homolog (KAU) mRNA, complete cds and for DDBJ Acc.No D14883 | MUSC33R2IA: Mouse mRNA for C33 / R2 / IA4, complete cds with an identity of 94%. For clone 25, it was shown that C33-induced apoptosis is mediated by the induction of oxygen radicals, which lead to proapoptotic activation of mitochondria. These oxygen radicals are not generated by the mitochondrial respiratory chain, since cells with a genetic defect in the respiratory chain still respond to C33 expression with apoptosis.

The proapoptotic activity of C33 is not dependent on substrate or cell-cell interaction, since suspension cells also become apoptotic by C33. The rapid kinetics of the apoptosis induction of C33 also differs from the previously claimed proapoptotic effects of C33. Furthermore, it was shown that an extracellular loop in C33, which apparently is responsible for the substrate interaction, is not necessary for the induction of apoptosis.

Clone 26 shows homology to Genbank Acc. No U50987 | BTU50987: Bos taurus succinate-ubiquinone reductase membrane anchor subunit precursor QPs3 mRNA, complete cds with an identity of 86% and DDBJ AB006202: Homo sapiens mRNA for cytochrome b small subunit of complex II, complete cds with an identity of 82%.

Clone 27 shows homology to Genbank Acc. No M31775 | MUSCYTB558: Mouse cytochrome beta-558 mRNA, 3 'end with an identity of 88%.

Clone 28 shows homology to Genbank Acc. No U76253 J MMU76253: Mus musculus E25B protein mRNA, complete cds with an identity of 88%.

Clone 29 shows homology to EMBL Acc. No Z98200.81 HS1 1 1 B22: Human DNA sequence from clone RP1-1 1 1 B22 on chromosome 6q16-21 Contains a novel pseudogene, a pseudogene similar to ribosomal protein L3, ESTs, STSs, GSSs and CpG Islands, complete sequence [ Homo sapiens] with an identity of 85% and Genbank Acc. No AW489000.1 | AW489000: UI-M-BH3-asd-a-10-0-Ul.s1 NIH_BMAPJV1_S4 Mus musculus cDNA clone UI-M-BH3-asd-a-10-0-UI 3 'with an identity of 85% and to Genbank Acc. No AW488401 .1 | AW488401: UI-M-BH3-art-b-02-0-Ul.s1 NIH_BMAP_M_S4 Mus musculus cDNA clone UI-M-BH3-art-b-02-0-UI 3 'with an identity of 98%.

Clone 30 shows homology to the following database entries: Genbank Acc. No AI1 15883 | AI1 15883: ue96a12.y1 Sugano mouse embryo mewa Mus musculus cDNA clone 1498942 5 ', mRNA sequence [Mus musculus] with an identity of 97%; Genbank Acc. No AA052396 J AA052396: mb67c07.r1 Soares mouse p3NMF19.5 Mus musculus cDNA clone 334476 5 'with an identity of 96%; Genbank Acc. No AA777720 JAA777720: zj06a08.s1 Soares fetal liver spieen 1 NFLS S1 Homo sapiens cDNA clone 449462 3 'with an identity of 91%; Genabank Acc. No AA9601 16JAA9601 16: ub54e08.s1 Soares mouse mammary gland NMLMG Mus musculus cDNA clone 1381574 3 'with an identity of 91%. Clone 31 shows homology to the following database entries: Genbank Acc. No AI31 5450] AI31 5450: uj46g05.y1 Sugano mouse liver mlia Mus musculus cDNA clone IMAGE-.1 923032 5 ', mRNA sequence [Mus musculus] with an identity of 89%; Genbank Acc. No AI01 96781 AI01 9678: ua92d1 1 .r1 Soares mouse mammary gland NbMMG Mus musculus cDNA clone 1 364949 5 'with an identity of 89%;

Clone 32 shows homology to Genbank Acc. No U071 59 | MMU071 59: Muscle medium-chain acyl-CoA dehydrogenase mRNA, complete cds with an identity of 94%.

Clone 33 shows homology to Genbank Acc. No M761 31 {MUSEF2: Mouse elongation factor 2 (ef-2) mRNA, 3 'end with an identity of 89)%.

Clone 34 shows homology to Genbank Acc. No AF072757 J AF072757: Mus musculus fatty acid transport protein 2 mRNA, complete cds with an identity of 96%.

Clone 35 shows homology to EMBL Acc. No X76453 J RNHREV1 07: R.norvegicus (Sprague Dawley) H-rev107 mRNA with an identity of 90% and EMBL Acc. No X92814J HSHREV107: H. sapiens mRNA for rat HREV107-Iike protein with an identity of 85%.

Clone 36 shows homology to Genbank Acc. No AW107362.1 | AW107362: um1 5a04.x1 Sugano mouse kidney mkia Mus musculus cDNA clone IMAGE: 21 92334 3 'similar to SW: ACY2_H UMAN P45381 ASPARTOACYLASE with an identity of 93%.

Clone 37 shows homology to the following database entries: Genbank Acc. No AI31 5920 | AI31 5920: uj27f1 1 .y1 Sugano mouse kidney mkia Mus musculus cDNA clone IMAGE: 1 921 1 97 5 'similar to TR: Q14521 Q14521

GIANT LARVAE HOMOLOGUE; mRNA sequence [Mus musculus] with one Identity of 87%; Genbank Acc. No A1315072 J AI315072: uj ^' 23e1 1.x1 Sugano mouse kidney mkia Mus musculus cDNA clone IMAGE: 1920812 3', mRNA sequence [Mus musculus] with an identity of 94%; Genbank Acc. No AF1099051 MMHC213L3: Mus musculus major histocompatibility locus class III regions Hsc70t gene, partial cds; smRNP, G7A, NG23, MutS homolog, CLCP, NG24, NG25, and NG26 genes, complete cds; and unknown genes with an identity of 82-96%; Genbank Acc. No AF1 10520 | MMHC425O18: Mus muscle major histocompatibility complex region NG27, NG28, RPS28, NADH oxidoreductase, NG29, KIFC1, Fas-binding protein, BING 1, tapasin, RalGDS-like, KE2, BING4, beta 1, 3-galactosyl transferase, and RPS18 genes with an identity of 82%.

Clone 38 shows homology to Genbank Acc. No S45663 J S45663: SC2 = synaptic glycoprotein [rats, brain, mRNA, 1 178 nt] with an identity of 91%.

Clone 39 shows homology to Genbank Acc. No AA763399 J AA763399: vw53h02.r1 Soares mouse mammary gland NMLMG Mus musculus cDNA clone 1247571 5 'similar to WP: F32D8.4 CE05783 LACTATE DEHYDROGENASE with an identity of 96%.

Clone 40 shows homology to Genbank Acc. No U00677 | U00677: Mus musculus syntrophin-1 gene, complete cds with an identity of 92%.

Clone 41 shows homology to Genbank Acc. No AC003043 J AC003043: Homo sapiens chromosome 17, clone HRPC1067M6, complete sequence [Homo sapiens] with an identity of 87% and to Genbank Acc. No AA109006 | AA109006: ml63d04.r1 Stratagene mouse testis (# 937308) Mus musculus cDNA with an identity of 95% and to Genbank Acc. No AA985996 J AA985996: Sugano mouse liver mlia Mus musculus cDNA clone 1431282 3 'similar to TR: O14589 014589 SIMILARITY TO Q09461 with an identity of 93%. Clone 42 shows homology to Genbank Acc. No U30838 | MMU30838: Mus musculus voltage dependent anion Channel 2 mRNA, nuclear gene encoding mitochondrial protein, complete cds with an identity of 94% as well as Genabank Acc. No L08666 J HUMPORIN: Homo sapiens porin (por) mRNA, complete cds and truncated cds with an identity of 94%:

Clone 43 shows homology to the following database entries: Genbank Acc. No M27071 J MUSDIS2M1 A: Mus muscle protein phosphatase type 1 (dis2m1) mRNA, complete cds with an identity of 92%; Genbank Acc. No U53456 | MMU53456: Muscle protein phosphatase 1 cgamma (PP1 cgamma) mRNA, complete cds with an identity of 98-100%; EMBL Acc. No X56438 J DMPP1A1: D.melanogaster PP1 -alpha 96A gene for protein phosphatase 1 with an identity of 80%; Genbank Acc. No M27067 J EMEBIMG: Aspergillus nidulans phosphoprotein phosphatase 1 mRNA, complete cds with an identity of 80%; Genbank Acc. No M60215 J MZEZMPP1: Z.mays protein phosphatase-1 (ZmPPD mRNA, complete cds with an identity of 83%; Genbank Acc. No U00063 J CELF56C9: Caenorhabditis elegans cosmid F56C9 with an identity of 81 -86%.

Clone 44 shows homology to Genbank Acc. No U52842 | MMU52842: Muscle kidney-specific transport protein mRNA, complete cds with an identity of 99% and on DDBJ Acc. No AB004559] AB004559: Rattus norvegicus mRNA for multispecific organic anion transporter, complete cds with an identity of 93%.

Clone 45 shows homology to the following database entries: Genbank Acc. No AF080252 J AF080252: Mus muscle serine / threonine protein kinase 51 PK (S) mRNA, complete cds with an identity of 94%; Genbank Acc. No AF080253 | AF080253: Mus muscle serine / threonine protein kinase 51 PK (L) mRNA, complete cds with an identity of 94%; DDBJ Acc. No AB000449 J AB000449: Homo sapiens mRNA for VRK1, complete cds with an identity of 83%.

Clone 46 shows homology to the following database entries: Genbank Acc. No J04806 j MUSOSP: Muscle osteopontin mRNA, complete cds with an identity of 95%; EMBL Acc. No X1 3986 | MMPONTIN: Mouse mRNA for minopontin with an identity of 96%; EMBL Acc. No X 1 61 51 | MMETA1: Mouse mRNA for early T-lymphocyte activation 1 protein (ETa-1) with an identity of 95%; Genbank Acc. No S781 77 1 S781 77: Eta-1 / Op (Eta-1 b) = early T-lymphocyte activator-1 [mice, C3H / HeJ, spieen, mRNA, 1087 nt] with an identity of 98%.

Clone 47 shows homology to DDBJ Acc. No AB006451 | AB006451: Rattus norvegicus mRNA for Tim23, complete cds with an identity of 94% and Genbank Acc. No AF0301 62 J AF0301 62: Homo sapiens inner mitochondrial membrane translocase Tim23 (TIM23) mRNA, nuclear gene encoding mitochondrial protein complete cds with an identity of 89%.

Clone 48 shows homology to DDBJ Acc. No D38549 J HUMHA1025A: Human mRNA for KIAA0068 genes, partial cds with an identity of 86% and to Genbank Acc. No AF072697 J AF072697: Mus muscle SHYC (Shyc) mRNA, complete cds with an identity of 96%.

Clone 49 shows homology to Genbank Acc. No U07971 j RNU07971: Rattus norvegicus Sprague-Dawley L-arginine: glycine amidinotransferase mRNA, partial cds with an identity of 85%.

Clone 50 shows homology to DDBJ Acc. No AU035342 j AU035342: Sugano mouse brain mncb Mus musculus cDNA clone MNCb-0343, mRNA sequence [Mus musculus] with an identity of 87% and to Genbank

Acc. No AI286459 J AI286459: ui77d03.y1 Sugano mouse liver mlia Mus musculus cDNA clone IMAGE: 1888421 5 'similar to WP: T06D8.9 CE02330, mRNA sequence [Mus musculus] with an identity of 87%.

Clone 51 shows homology to Genbank Acc. No M22998 | MUSGLUTRN: MUSGLUTRN Mouse facilitated glucose transport protein mRNA, complete cds with an identity of 93% as well as Genabnk Acc. No S77924J S77924: Glut-1 = glucose transporter isoform 1 [mice, embryo, mRNA partial, 321 nt] with an identity of 93%.

Clone 52 shows homology to EMBL Acc. No AJ010953 | HSA010953: Homo sapiens mRNA for putative Ca2 + -transporting ATPase, partial with an identity of 79%.

Clone 53 shows homology to Acc. No U94593: Mus muscle uncoupling protein homolog (UCPH) mRNA.

Clone 54 shows homology to Acc. No M2731 5.1: Rattus norvegicus mitochondrial cytochrome c oxidase subunits I, II and III, and ATPase subunit 6 genes, complete cds.

Clone 55 is composed of the T7 and BGH sequences. The total sequence is given under total sequence. Clone 12 shows homology to the following database entries: Acc. No AL080317.1 1: Human DNA sequence from clone RP5-1 1 12D6 on chromosome 6q21-22.2. Contains the gene for a PUTATIVE novel protein similar to bacterial NARK (nitrite extrusion protein, nitrite facilitator), the 3 'end of the REV3L gene for REV3 (yeast homologHike, catalytic subunit of DNA polymerase zeta (EC 2.7.7.7, POLZ), ESTs, STSs, GSSs and a putative CpG island, complete sequence. Clone 56 shows homology to Acc. No AW106096.1: um23a10.y1 Sugano mouse embryo mewa Mus musculus cDNA clone 1MAGE: 2225370 5 ', mRNA sequence.

Clone 57 shows homology to Acc. No NM_000210.1 Homo sapiens integrin, alpha 6 (ITGA6) mRNA.

Clone 58 shows homology to Acc. No AW106096. 1: um23a 10.y1 Sugano mouse embryo mewa Mus musculus cDNA clone IMAGE: 2225370 5 ', mRNA sequence.

Clone 59 shows homology to Acc. No NM_007748.1: Mus muscle cytochrome c oxidase, subunit VI a, polypeptide 1 (Cox6a1), mRNA.

Clone 60 shows homology to Acc. No AC005403.1: Mus musclulus clone UWGC: ma53a068 from 14D1 -D2 (T-Cell Receptor Alpha Locus), complete sequence.

Clone 61 shows homology to Acc. No L07095.1: Mus domesticus strain NZB / B1 NJ mitochondrion genome, complete sequence.

Clone 62 shows homology to Acc. No NM_0091 55.1: Mus muscle selenoprotein P, plasma, 1 (SeppD, mRNA complete cds.

Clone 63 shows homology to Acc. No J03297.1: Mouse ERp99 mRNA encoding an endoplasmic reticulum transmembrane protein.

Clone 64 shows homology to Acc. No AF047431 .1: Homo sapiens AAPT1-Iike protein mRNA, partial cds.

Clone 65 shows homology to EMBL Acc. No U79287 Human clone 23867 mRNA sequence 1 396 bp. Clone 66 shows homology to Acc. No C88489, AA073437, AI048028 and to ESTs with the DDBJ Acc. No C88489; Genbank Acc. No AA073437; AI048028.

Clone 67 shows homology to Acc. No AK001441: Homo sapiens cDNA FLJ 10579 fis, clone NT2RP2003446 2251 bp mRNA and to ESTs with the Acc. No AI663355 and AU080732.

Clone 68 shows homology to Acc. No AF005038: Homo sapiens Secretory Carrier Membrane Protein (SCAMP2) mRNA

Clone 69 shows homology to Acc. No U44731: Putative purine nucleotide binding protein mRNA muscle.

Clone 70 shows homology to Acc. No U 1 681 8: Muscle UDP glucuronosyltransferase (UGT1 -06) mRNA

Clone 71 shows homology to Acc. No NM_004256: Homo sapiens organic cationic transporter-like 3 (ORCTL3) mRNA.

Clone 72 shows homology to Acc. No AK000559: Homo sapiens cDNA FLJ20552 fis, clone KAT1 1 732.

Clone 73 shows homology to Acc. No X89968: Rattus norvegicus mRNA for alpha-soluble NSF attachment protein.

Clone 74 shows homology to Acc. No D851 37: Mouse mRNA for PPI gamma (protein phosphatasel gamma).

Clone 75 shows homology to Acc. No NM_001089: Homo sapiens ATP-binding cassette, sub-family A (ABC1), member 3 (ABCA3) mRNA. Clone 76 shows homology to mouse ESTs with the Acc. No AI315969, AI930239, A1666299 and AV141 103.

Clone 77 shows homology to Acc. No AB025405: Mus musculus mRNA for sid2895p (Mouse microsomal signal peptidase)

Clone 78 shows homology to Acc. No AK000427. The clone is partially homologous to: Homo sapiens cDNA FLJ20420 fis, clone KAT02462.

Clone 79 shows homology to Acc. No NM_009127: Mus musculus stearoyl-Coenzyme A desaturase 1 (Scd1)

Clone 80 shows homology to Acc. No U21049: Human DD96 mRNA (a gene selectively upregulated in human carcinomas).

Clone 81 shows homology to Acc. No AF070626 or AB020980: Homo sapiens clone 24483 unknown mRNA (AF070626), or Homo sapiens mRNA for putative membrane protein (AB020980).

Clone 82 shows partial homology to Acc. No U92989: Homo sapiens clone DT1 P1 E1 1 mRNA, CAG repeat region.

Clone 83 shows homology to Acc. No AF035208: Mus muscle putative v-SNARE Vtil b mRNA (soluble NSF attachment protein receptor).

Clone 84 shows homology to Acc. No NM_012504: Rattus norvegicus ATPase, Na + K + transporting, alpha 1 polypeptide (Atp1 a1), mRNA.

Clone 85 shows homology to Acc No NM_007749: Mus musculus cytochrome c oxidase subunit Vllc (Cox7c), mRNA. Clone 86 shows homology to Acc No AF079565: Mus ubiquitin-specific protease UBP41 (Ubp41) mRNA.

Clone 87 shows homology to Acc No AJ006341: Mus musculus mRNA for peroxisomal integral membrane protein PMP34.

Clone 88 shows homology to Acc No AF223950: Mus musculus TIM22 preprotein translocase (Tim22) mRNA, complete cds; nuclear gene for mitochondrial product.

Clone 89 shows homology to Acc No NM_01 1479: Mus muscle serine palmitoyltransferase, long chain base subunit 2 (Sptlc2), mRNA.

Clone 90 shows homology to Acc No M23984: Rat mitochondrial proton / phosphate symporter mRNA, complete cds and Acc. No X60036: H. sapiens mRNA for mitochondrial phosphate carrier protein.

Clone 91 shows homology to Acc No AF1 13127: Homo sapiens S1 R protein (S1 R) mRNA, complete cds (human cowpox virus homolog).

Clone 92 is composed of the T7 and BGH sequences. The entire sequence is shown as a merging of several EST sequences (“EST assembly”) in Contig. Clone 92 shows homology to UniGene Mm.86545.

Clone 93 shows homology to human genomic DNA (PAC 69E1 1 on chromosome 1 q23-24).

Clone 94 shows homology to Acc No NM_01 1512: Mus musculus surfeit gene 4 (Surf4), mRNA. Clone 95 is composed of the T7 and BGH sequences. The entire sequence is shown as a combination of several EST sequences (“EST assembly”) under total cDNA EST clusters. Clone 95 shows homology to UniGene: Mm.27841 (mouse) and Hs.100132 (human).

Clone 96 shows homology to UniGene Hs.133494, Mm.31778 and Rn.1 1778.

Clone 97 shows homology to Acc No AF192558: DEFINITION Mus musculus domesticus mitochondrial carrier homolog 1 isoform b (Mtchl) mRNA, complete cds; nuclear gene for mitochondrial product and Acc. No AF192558 / AF192559: Homo sapiens mitochondrial carrier homolog 1 isoform b (MTCH1) mRNA, partial cds; nuclear gene for mitochondrial product.

Clone 98 shows homology to Acc No NM_016783: Mus musculus progesterone receptor membrane component (Pgrmc-pending), mRNA.

Clone 99 shows homology to Acc No NM_003002: Homo sapiens succinate dehydrogenase complex, subunit D, integral membrane protein (SDHD) mRNA.

Clone 100 shows homology to Acc No U95822: Human putative transmembrane GTPase mRNA, partial cds.

Clone 101 shows homology to Acc No X99963: M. musculus rhoB gene.

Clone 102 shows homology to Acc No AF161 525: Homo sapiens HSPC177 mRNA, complete cds. The gene contains a BH3 domain that is found in many Bcl-2-like genes. Genes with such "BH3-only" domains are pro-apoptotic and have the evolutionarily conserved function of inducing apoptosis. The experiments showed that CGl-135 with Bcl-2 gene family members can interact. The BH3 domain is necessary for efficient apoptosis induction by CGl-135.

Clone 103 shows no homology to known sequences.

Clone 104 shows homology to Unigene Acc No AW682500.30119 Mm.30119jMm.30119: Mus musculus cDNA with an identity of 91%.

Clone 105 shows homology to EMBL Acc No X98475.1 jMMVASP: M. musculus VASP gene with an identity of 90%.

Clone 106 shows homology to EMBL Acc No X03369.1 | RNTUBB15: Rat mRNA for beta-tubulin T beta15 with an identity of 90%.

Clone 107 shows homology to Genbank Acc No BE226644.1 JBE226644: ia23h11.y1 Mouse E10 5 12 5 Pancreas cDNA Library Mus musculus cDNA 5 'similar to SW: NUML_BOVIN Q01321 NADH-UBIQUINONE OXIDOREDUCTASE MLRQ SUBUNIT with an identity of 87%.

Clone 108 shows homology to Genbank Acc No AF157317.1 | AF157317: Homo sapiens AD-015 protein mRNA, complete cds with an identity of 83%.

Clone 109 shows homology to Genbank Acc No M88136.1 | CRUSTSTA: Cricetulus griseus seryl tRNA synthetase mRNA, partial cds with an identity of 91%.

Clone 110 lacks homology to known sequences.

Clone 111 shows homology to Genbank Acc No AW106096.27359 j Mm.27359: um23a10.y1 Mus musculus cDNA, 5 'end with an identity of 99%. Clone 112 shows homology to Genbank Acc No AI413025.1 | AI413025 EST241326 Normalized rat brain, Bento Soares Rattus sp. cDNA cloneRBRDZ283 'end with 97% identity.

Clone 113 shows homology to EMBL Acc No Human DNA sequence from clone RP3-402G11 on chromosome 22q13.31 -13.33 "Contains the MAPK12 gene for mitogen activated protein kinase 12 (SAPK3), the MAPK11 gene for mitogen activated protein kinase 11 (PRKM11) gene KIAA0315, the gene for a novel protein "with an identity of 86%.

Clone 114 shows homology to DDBJ Acc No AK000823.11 AK000823 Homo sapiens cDNA FLJ20816 fis, clone ADSE00693 with an identity of 88%.

Clone 115 shows homology to Genbank Acc No AF007152.11 AF007152 Homo sapiens clone 23649 and 23755 unknown mRNA, partial cds with an identity of 83%.

Clone 116 shows homology to EMBL Acc No X66370.1 JRNRPS9 R. norvegicus mRNA for ribosomal protein S9 with an identity of 94%.

Clone 117 shows homology to Genbank Acc No BE161116.11 BEI 61116 PM3-HT0424-170200-001-b 11 HT0424 Homo sapiens cDNA with an identity of 100%.

Clone 118 shows homology to DDBJ Acc No AK000559.1 JAK000559 Homo sapiens cDNA FLJ20552 fis, clone KAT11732 with an identity of 86%.

Clone 119 shows homology to RefSeq Acc No NM_003713.1 | Homo sapiens phosphatidic acid phosphatase type 2b (PPAP2B) mRNA, and translated products with an identity of 87%. Clone 120 shows homology to Genbank Acc No M30773.1 (HUMCNR: Human calcineurin B mRNA, complete cds with an identity of 88%.

Clone 121 shows homology to RefSeq Acc No NM_013770.1 or Genbank Acc. No AF188712.KAF188712: Mus muscle solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 10 (Slc25a10), mRNA and Mus muscle mitochondrial dicarboxylate carrier mRNA, complete cds; nuclear gene for mitochondrial product with an identity of 98%.

Clone 122 shows homology to RefSeq Acc No NM_008525.1: Mus musculus delta-aminolevulinate dehydratase (Lv), mRNA with an identity of 100%.

Clone 123 shows homology to Genbank Acc No M59861.KRAT10HCO: Rattus norvegicus 10 formyltetrahydrofolate dehydrogenase mRNA, complete cds with an identity of 93%.

Clone 124 shows homology to the following database entries: EMBL Acc No X00525.1 (MMRNA02: Mouse 28S ribosomai RNA with an identity of 84%; Genbank Acc. No AF061799.KAF061799: Hydrolagus colliei internal transcribed spacer 1, partial sequence; 5.8S ribosomai RNA gene and internal transcribed spacer 2, complete sequence; and 28S ribosomai RNA gene, partial-sequence with an identity of 83%; Genbank Acc. No AF061800.1 (AF061800: Squalus acanthias internal transcribed spacer 2; and 28S ribosomai RNA gene, partial sequence with an identity of 83%.

Clone 125 shows homology to RefSeq Acc. No NM_007469.1: Mus muscle apolipoprotein Cl (Apod), mRNA as well

AK011358.1 | AK011358 Mus musculus 10 days embryo cDNA, RIKEN full-length enriched library, clone: 261 0009E08, fill insert sequence with an identity of 1 00%.

Clone 1 27 shows homology to Genbank Acc. No AK007676: Mus musculus 10 day old male pancreas cDNA, RIKEN full-length enriched library, clone: 1 81 0032P22, fill insert sequence with an identity of 1 00%; to Genbank Acc. No BC002242: Mus musculus, Similar to sorting nexin 5, clone MGC: 7534, mRNA, complete cds with an identity of 100% and Genbank Acc. No AC008066: Homo sapiens BAC clone RP1 1 -293D9 from 8, complete sequence.

Clone 1 33 shows homology to Genbank Acc. No AK01 5068.1 J AK01 5068: Mus musculus adult male testis cDNA, RIKEN full-length enriched library, clone: 4930403O06, fill insert sequence with an identity of 90% and to DDBJ Acc. No AK004504.1 1 AK004504: Mus musculus 18 days embryo cDNA, RIKEN full-length enriched library, clone: 1 1 90006A08, fill insert sequence

Clone 1 34 shows homology to Genbank Acc. No BC002108.1 {BC002108: Mus musculus, Similar to ribosomai protein

S27a, clone MGC: 6601, mRNA, complete cds with 98% identity.

Clone 140 shows homology to DDBJ Acc. No AK008346.1 | AK008346 Mus muscle adult male small intestine cDNA, RIKEN full-length enriched library, clone: 2010107K03, fill insert sequence with an identity of 99% and for RefSeq Acc. No NM_007512.1 j Mus muscle ATPase inhibitor (Atpi), mRNA with an identity of 99%.

Clone 141 shows no homology to known sequences.

Clone 1 44 shows homology to Genbank Acc. No AF006482.1 J AF006482 Mus nucleoside triphosphatase muscle (NTPase) mRNA, complete cds with 99% identity; to Genbank Acc. No AF084569. 1 | AF084569 Mesocricetus auratus cph proto-oncogene product (cph) mRNA, complete cds with an identity of 90%.

Clone 145 shows homology to DDBJ Acc. No AK002787.1 J AK002787 Mus musculus adult male kidney cDNA, RIKEN full-length enriched library, clone: 0610038B1 2, fill insert sequence with an identity of 1 00%; to DDBJ Acc. No AK010989.1 | AK010989 Mus musculus 1 3 days embryo liver cDNA, RIKEN full-length enriched library, clone: 2510022D06, fill insert sequence with an identity of 100%; to RefSeq Acc. No NM_031 101 .1 | Rattus norvegicus ribosomai protein L13 (Rpl13), mRNA with an identity of 93%.

Clone 146 shows homology to Refseq Acc. No NM_025278.1 | Mus muscle guanine nucleotide binding protein (G protein), gamma 1 2 (Gng1 2), mRNA with an identity of 99%.

The apoptosis-inducing genes identified by the genetic screen (clones 1 - 1 24) are listed in Table 1 (pages 1 - 125) attached as an illustration:

The information in Table 1 is defined as follows:

"T7 sequence": 5 'side sequence of the clone "BGH sequence": 3' side sequence of the clone

"Internal primer": internal sequences of the clone identified using primers obtained from the T7 sequence (left) or the BGH sequence (right) "identity": comparison with sequences from the computer program

"BLAST" (Altschul et al. (1,997), Nucleic Acids Res. 25: 3389-3402). In addition to completely identical sequences (Identity 100%) partially identical sequences (identity of preferably ≥ 85%) are also given, which show allelic variants of the specifically shown sequence or homologous sequences from other species, in particular from humans.

Such variants or homologous sequences are of course also covered by the present invention.

"contig" complete sequence; put together from the "T7 sequence", "BGH sequence and optionally from the" internal primer "sequences

"Properties" Information about the predicted properties of the resulting peptide

, Prediction "An analysis with the computer program" PSORT II "(Nakai, K. and Kanehisa, M., Genomics 14: 897-91 1 (1 992)) determines possible properties and the localization in the resulting peptide from the sequence Cell with a predicted probability. For clone 1, for example, two transmembrane domains and a location in the ER (endoplasmic reticulum) with a probability of 33%, in the vacuole with a probability of 22%, in the mitochondria with a probability of 1 1% and in the Golgi apparatus with a probability determined by 1 1% "DNA leader" Biochemical detection for apoptosis (Bitzer et al.

(1999) J. Virol. 73: 702-708)

"PI-Facs" Propidium iodide-Facs ": Detection for apoptosis (Bitzer et al. (1999) J. Virol. 73: 702-708)

"Possible ORF" Specification of a possible "open reading frame" (ORF) in the one-letter code

The apoptosis-induced genes identified by the genetic screen (clones 1-124, 125, 127, 133, 134, 140, 141, 144, 145, 146) are listed in Table 2 (pages 1-28) attached as an illustration.

Table 1

- -

4- 'Jl

- see

'Jl

00

it

-4

©

'Jl

-4 -4

©

Convention for the evaluation of the phenotypic 'cell death effect':

- negative, - / + and - / ++ weakly positive, + positive, ++ strongly positive, +++ very strongly positive

Clone # 53

T7 sequence GCTTGACCTTGGAGGCCTCAGCCTGAGACCTCAAAGCAGCCTCCAGAACTCCGGCAGAGTTCCTCTGTCTCGTCTTGCCG

ATTGMGGTCCCCGTTTCTCC TTTCTCTCCATCTTCTGGGAGGTAGCAGGAAATCAGAATCATGGTTGGTTTCAAGGCC

CAGATGTGCCCCCAACAGCCACTGTGAAGTTCCTGGGGGCTGGGACAGCTGCCTGCATTGCAGATCTCATCACTTTCCCT

CTGGATACCGCCAAGGTCCGGCTGCAGATCCAAGGGGAGAGTCAAGGGCTAGTGCGCACCGCAGCCAGCGCCCAGTAC

GTGGCGTTCTGGGTACCATCCTAACCATGGTGCGCACTGAGGGTCCACGCAGCCTCTACAATGGGCTGGACGCCGGCCT

GCAGCGCCAGATGAGCTTTGCCTCCGTCCGCATTGGCCTCTACGACTCTGTCAAACAGTTCTACACCAAGGGCTCAGAGC

ATGCAGGCATCGNGAGCCGCTTCTGGCAGGTAGCACCACAGGTGCCTTGNCGTGGCTGTNGCCCNAGCCTACAGATGTN

NAAAGGCCCGTTTC

BGH sequence: GCCAGCCTAACACTTCTATGACAAACCGANGAAAATTATAAAAACTTTTCAAATATATTGCTCCTCAGACCCCCTATCCACA

CATTAATTATTTTAACAGCCTGATTACTGCCACTAATATTAATAGCTAGCCAA CCACCTAAAAAAAGATAATAACNGTACT

CAAA CTCTACATCTC TACT TCAGCTTACAAATTCTCCT TCATMCCTTTTCAGCAACTGAACTAATTATATTTTAT

TTTTATTTGAAGCAACCTTAATCCCAACACTTATTATTATTACCCGATGAGGGAACCAAACTGAACGCCTAAACGCAGGGAT

TATTTCCTATTTTATACCCTAATCGGTTCTATTCCACTGCTAATTGCCCTCATCTTAATCCAAAACCATGTAGGAACCCTAAA

CTCATMTTTTATCATTCACAACACACACCTTAGACGCTTCATGATCTAACAACTTACTATGGTTGGCATGCATAATAGCATT

CTTATTANAATACCATTATATGGAGTTCACCTATGACTACCAAAAGCCCATGTTGAAGCTCCAATTGCTGGGTCAATAATTCT

AGCAGCTATTCTTCTAAAATTAGGTAGTTACGGAATAATTCGCATCTCC

Running clone number: # 7 ACCESS No .: M27315.1 GL343181 definition: Rattus norvegicus mitochondrial cytochrome c oxidase subunits I, II and III, and ATPase subunit 6 genes, complete cds, and Trp-, Ala- Asn-, Cys-, Tyr, Ser, Asp, Lys, Gly, Arg tRNA genes.

LOCUS: RATMTCYTOC 7632 bp DNA ROD 29-MAY-1996 Effect: ++

Clone # 55

T7 sequence GGATTGGCGGGGTTCTCTTTGACCACATAAATCAATTCTTTCTTTTGGGAGCCTCAATGGTGGCAACTGCAGCGGGACTTT

ATCTCATCCCGTTCTGCAAGACAGCAGTCTTATTGATCATCACCATGTCTGTCTTTGGAGCTTCCGTTGGTGTTGTGGATAC

AGGTGCAAATGTTCTCATCTTGGATCTCTGGGGGGACAAAGGAGCCCCACAAATGCAGGCCTTGCACTTCAGTTTCGCCTT

GGGTGCCTTTCTGGCTCCCCTGCTGGCTAAGTTGGCCTGGGGTACAGCACCTGCTCAGAACCACACCGAGTCCGACCTTG

ACACTCTGATGCTGAACCGATCCTCCAACGGCACCTCAGACTCCGTGTTTGCGGTACCCGATGACATGAATCTGCTGNGG

GCATATGCTTCCATTGGCACCTTTATTTTAGTAGTTTCTGTCTTTCTGNNNGGTCTGTTTTGTAAGAAACATTCAAGGCAGAA

AAAACCCAGAGCATCTGCTGAGGGAGCTCGAAGGGCTAAATATCACAGGGCCCTGCTATGCCTGCTCTTCCTCTTCTTCTT

CTTCTATGTGGGAGCCGAGATAACATACGGCTCTTACATATTCTCCTTCGCCACCACCCATGTTGGCATGGAAGAGAGCGA

GGCAGCTGGCTTGAATTCCATCTTCTGGGGAACCTTTGCAGCCTGCAGGGGCCTGGCCATCTTCTTTGCGACATTCTTACA

GCCTGGAACCATGATCGTGCTGAGCAACATTGGCAGCCTGGNCTCATGTTTCTTTCTGGTACT

BGH sequence: AAATATCACA GGGCCNTGNT AGCCTGCTNT TCCCTCTTCT TCTTCTTCTA NTGGAANCCG AAGNTACCAT NNCGNTTCTT ANCAGTTTCT CCTTTCGGCC NCCNACCCAG TGGCATGGAA GAGAGCGAGG CAGCTGGCTT GAATTCCCAT CTTCTGGGGA ACCTTTGCAG CCTGCAGGGG CCTGGCCATC TTCTTTGCGA CATTTCTTAC AGCCTGGANC CATGATCGTG CTGAGCAACA TTGGCAGCCT GGTCTCATGT TTCTTTCTGG TACTTTTTGA CAAGAGCCCT CTTTGTCTCT GGATCGCGAC TTCTGTGTAT GGAGCCTCAA TGGCAGCCAC GTTTCCCAGC GGCATCTCCT GGATTGAGCA GTACNCCNCC TTAACTGGGA AATCTGCAGC ATTCTTTGTA ATTGGCTCTG CCCTGGGAGA TATGGCCATT CCAGCGGTGA TCGGAATTCT TCAGGGACNC TACCCAGATC TGCCAGTAGT TCTGTACACA TGTCTGGGCT CAGCCATATT CACAGCTATT TTATTTCCTG TGATGTATAA ATTAGCTACC TTGCCCCTGA AGAGAGAGGA CCAGAAAGCT TTGCCCACTA GTTCTAGACT GTGAGGAAGA GACTACATGA GAACTTAAAA AAAAAAAAAA AGGGCGGCCG CTCGAGCATG CATCTAGAGG GCCCTATTCT ATAGTGTCAC CTAAATGCTA GAGCNCNNCT GTCA

Overall sequence GGATTGGCGGGGTTCTCTTTGACCACATAAATCAATTCTTTCTTTTGGGAGCCTCAATGGTGGCAACTGC composed (from T7 AGCGGGACTTTATCTCATCCCGTTCTGCAAGACAGCAGTCTTATTGATCATCACCATGTCTGTCTTTGGA and BGH) GCTTCCGTTGGTGTTGTGGATACAGGTGCAAATGTTCTCATCTTGGATCTCTGGGGGGACAAAGGAGCCC

CACAAATGCAGGCCTTGCACTTCAGTTTCGCCTTGGGTGCCTTTCTGGCTCCCCTGCTGGCTAAGTTGGC

CTGGGGTACAGCACCTGCTCAGAACCACACCGAGTCCGACCTTGACACTCTGATGCTGAACCGATCCTCC

AACGGCACCTCAGACTCCGTGTTTGCGGTACCCGATGACATGAATCTGCTGNGGGCATATGCTTCCATTG

GCACCTTTATTTTAGTAGTTTCTGTCTTTCTGNNNGGTCTGTTTTGTAAGAAACATTCAAGGCAGAAAAA

ACCCAGAGCATCTGCTGAGGGAGCTCGAAGGGCTAAATATCACAGGGCCCTGCTATGCCTGCTCTTCCTC

TTCTTCTTCTTCTATGTGGGAGCCGAGATAACATACGGCTCTTACATATTCTCCTTCGCCACCACCCATG

TTGGCATGGAAGAGAGCGAGGCAGCTGGCTTGAATTCCATCTTCTGGGGAACCTTTGCAGCCTGCAGGGG

CCTGGCCATCTTCTTTGCGACATTCTTACAGCCTGGAACCATGATCGTGCTGAGCAACATTGGCAGCCTG

GTCTCATGTTTCTTTCTGGTAC I I I I 1 GACAAGAGCCCTCTTTGTCTCTGGATCGCGACTTCTGTGTATG

GAGCCTCAATGGCAGCCACGTTTCCCAGCGGCATCTCCTGGATTGAGCAGTACNCCNCCTTAACTGGGAA

ATCTGCAGCATTCTTTGTAATTGGCTCTGCCCTGGGAGATATGGCCATTCCAGCGGTGATCGGAATTCTT

CAGGGACNCTACCCAGATCTGCCAGTAGTTCTGTACACATGTCTGGGCTCAGCCATATTCACAGCTATTT

TATTTCCTGTGATGTATAAATTAGCTACCTTGCCCCTGAAGAGAGAGGACCAGAAAGCTTTGCCCACTAG

TTCTAGACTGTGAGGAAGAGACTACATGAGAACTTAAAAAAAAAAAAAAAGGGCGGCCGCTCGAGCATGC

ATCTAGAGGGCCCTATTCTATAGTGTCACCTAAATGCTAGAGCNCNNCTGTCA

Running clone number: # 13 ACCESS No .: AL080317.11 Gϊ.5830430 Definition: Human DNA sequence from äone RP5-1 12D6 on chromosome 6q2 -22.2. Contains the gene for a PUTATIVE novel protein similar to bacterial NARK (nitrite extrusion protein, nitrite facilitator), the 3 'end of the REV3L gene for REV3 (yeast homolog) -like, catalytic subunit of DNA polymerase zeta (EC 2.7.7.7, POLZ), ESTs, STSs, GSSs and a putative CpG island, complete sequence.

LOCUS HSJ1112D6 135305 bp DNA PRI 21-FEB-2000 Effect:

Running clone number: # 20 (EST) ACCESS No .: AW106096.1 Gl: 6076832 Definition: um23a10.y1 Sugano mouse embryo mewa Mus musculus cDNA clone

IMAGE: 2225370 5 ', mRNA sequence

LOCUS: AW106096 539 bp mRNA EST 20-OCT-1999

Effect: ++

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Clone # 57

T7 sequence GATAGTTTTGAACTTCCGATTCCCCTGCCTCCCCACCCCAGGGCTGTGATTGCAGGTGTGCGCTTGGGACTGACCCCAG

CTTTGTCGGTGCTAGGGCAGCACTCTGCGACTGMTTAGGTCCCAGCCACTTCTCTGTCTTTTAAAAGAACAAAACATTGC

AAATGTGCCATTGTTGCTTTGAGTTTTAATTCTTTTTTTTTTCTTTCTTTCATAAAAC

TATTCTGGTTCTATTΓCTGTCTTTTCACTCAAAACTGGTTTTACAAATGATGCCTTGTTTACAGAAAGCTCTCTACCACAGGG

CCTAGTCATGTGTAAAGTCTCAGTTTCTCTCTGGAGTATCTTGGAGCCTAGCACACTGGCTTTAAAGGACACAGCTAAGAA

CTGATATCTTGACAGTGTTTGTAGACCTTTGTTATAAAAATGAATGTCCTGGAAAGGGTTGGGAGGGAGTTCAACAACAAA

AAACAAGMTGTCATGTTTAAATTTAATAGTTGTCTAAAATGTCATCTC GTCAAGTCACTGGTCTGTTTGCATTTGATAGG

TTTTATACTAACTAGCATTATAAGATTATTTCATAATTAGAAAATACCTGTGGATATTTGTATAAAAGTGTGAAATAAAT ^

ACAAAAGTGCTCATCGCTTGTTAACACAGCATCATGTATGTGAAAGCAAACTCTAAGATTATAAATGACAACCTGAGTTGCC

TTCTTΓGTATTTCATCAAGCCNAAGTAAAGCTTTCANTATTTAAA

BGH sequence: n.d.

Running clone number: # 21

EST with low homology to human integrin

ACCESS No .: NM_000210.1 Gl: 4557674

Definition: Homo sapiens integrin, alpha 6 (ITGA6) mRNA.

LOCUS: NM_000210 5611 bp mRNA PRI 15-MAY-2000

Effect:

Clone # 58

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Clone # 59

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Clone # 60 T7 sequence GCATCCCTCCATGGCCTCTGCATTGGCTTCTGCTTTCTGACCTGCTTGAGTTCCAGTCCTGACTTCCTTGGNGATGAACAG

CAGTATGGAAGTGTAAGCCGMTAAACCCTTTCCTCTCCAACTTGTTTCTTGGTCATGATGNNTGTGCAGGAATAGAAACC

TGACT GACAAATTGGTACCAGCAGAGTGGGGTATTCCTGTGACAACCTGACCATGTTTTGGGGAGGACTGTGGATGGA

TTTGGAACTTTGGGCTTAAAGATCCATCCGNTGNTAAGAGCTCTGTCAGATGTTGTGTAGGAGCTTGGAAGATAATGTTGA

AACACTGNAGAANATGGAGGNCTGGTNTGNGAAATTTNAGAGGGAAAANTAAAGACTCTTTCAGGGCCATTGCTGTTTTGA

ATGTGAAGATTCTGTAGNNCTGGATAGCTGGGGCTNAANAATCANCTGTGNTTAACANGATCCAGAACTACCAANGCANAA

ACTTTGCATTACTGGGACTATTGATGCNTNNNTAGCTGNAGCTAACAAATTANCGGTGATTAANAAAGAGACCAGCNTCATT

NAGGGTGACATCTTTCTGGGNANGTGTTTCNTGAAAGCACANNGATNCNTGTGNTTNCAGAGATGGCCAAANGCTGTACC

CCTGCTTGCAANNANGGACTTGGGNATAANTGTAANNGGGTCNACCCNCNTNGNTACTGGGGTTTTTTAANGGCCNTTTA

CNGGGNTCNACGGCCACAGNCANCCTTCAGGGCCTNNGGCANCCTGGTTAAAAANGCCCCATTNNGAAANGGNCCAAAT

GNCTTTNAAAGGGTACNAGGCCTNCAAACTTGCCCAAATTGTANAGGGCCNCCCGGG

BGH sequence: GACCTGGCTT TNGCNCGCNN TNCANCCCTA GTCCCCCGGG TTCTCACTCC CTAAGCCCAT CGCAGNCCGG NTNGTGGANC CGCGCGTCCC AGGNTTCGTC CTTNCNCGGC CTNCAAGAAC ATGGCTTGCT TCAGAAAGAA AATAGTTTTG TCTTCTCTAA NAACTTACNT TCAGCTTGTC GAAGATGAAA ATAAAAAGCC CTGGAGAGGA ATAATTTCTT GCNCTTTATG AATCTATTTT TAAAATAAAA AAATTTACCN NCTTTNAATC TTTTTCCTCC TCNCAAAAGN AACCAGTATT TTTGCCTNCC ATTCANTTTG CNNCANTAAG ANNTTTGGAG CCTGAAACCN NAGNCTTTNT NANGGANTNT CNCCTTGGTT CAGCCTGNAG GCAAATCTGA TCAACGGACC TTTATGAGTC ATTTTTCCTA GACATATTCA GAAAACCTAG GAGCTGTGTC AAATGCCTGA ATTAAGCATT ACAAATGCAA GATNTTTGCN CTCTTGAAGA ATGTAGAGAG TAAAAGAACT ANAATTAAAA ANAATAANGC NTGTGATATA ACGGAATATA TATNTNAAAA ANAA

Clone # 62

T7 sequence GCAAACATGGNCAGGAGCATCTTGGCAGCNTTAAGCCTTCANAGAATTATCAACCANGGNCATNAGAGNCGACTCTGNCTC

NTTCAGGCTTGCACCACCACCACAGGCNTAGGGGCCAGCACAGGCAGGGTCACTTANAGAGCTGAGACACCACAGCAAG

NGAAGGCTNGCACCTTTCACTTGCCCAGAGGANGCTCTGACNAAGGGGGTGCATCAACCANCTCCNGTGTAAGCNGNCTA

AGGAGTCCGAGGCAGCCCCCANCAGCTGCTGCTGTCACTGCCGCCACCTCATATTTGANAAGTCANGGTCTGCANTATGC

TTGACAGTNTGCGNAAAACCTCCCATCCTTATGTANCTGACAGGNGCTTTTNCGCGNANTNACAAAAGCCACCTTGAACCC

TGTCANTNCTAGGTCACCTTCNAGCTTGCCTNGACNNAAANTCNNGTCCNTTGAAACCCCCNNTGGCANGCCCAAACCCCA

NNNTGAAGCTTNAGATNCTCAGGACNNCNNAANTTANANNNTGNCCATTTCNNACCTTANNNTTAATTTNAANNCTAAGGNC

ATTACTCTTNTTCCCCCCAAACNTNTAACNCCTTANANAGNACCNCCNNCTCTTTCCAATTNNTTNTNNCCAAATNGTNTNTN

NACCAGGTCCCCCAANTCTCAANNTNTAAATTTCTNNCTGNCAAACNCTTANANANTANGNTTTCCCTNNCCNAACAANTTN

TGNNCANACTNTNTNCGGNGNCCC

BGH sequence: n.d.

Running clone number:

# 48

ACCESS No .: Selenoprotein P

Definition: NM_009155.1 Gl: 7110716

LOCUS: Mus musculus selenoprotein P, plasma, 1 (Seppl), mRNA complete cds.

Seppl 2075 bp mRNA ROD 04-JAN-2000

Effect:

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-4

Clone # 63

T7 sequence GTTTTGTTTGAAACGGCAACACTTCGGTCANGATATCTTCTACCAGACACCAAGGCGTATGGAGATAGAATANAAAGAATG

TTCGCCTCAGNTTAAACATTGACCCTGAAGCACAGGNGGAGGAAGAACCNGAAGAAGAGCCTGAAGACACCTCAGAAGAC

GCANAAGACTCAGAGCAGGATGAGGNAGAAGAGATGGATGCAGGNACAGAANAAGAAGAGNGAGGAAACANATAAGGAA

CTACNGANAAGGATGAATTGTAANTTATACTCTCGCTATGAATCCCGNGTGGAGAGGNAATGNGAAGTTNTGAAGTCATTT

TTTTGAGAGACTTGNTTTGNATGCTTCCCCNNGCCTCCTTCTCCCCTGCNCTGTNAAATGNTTGGGATTNTGGGTCACAGG

AAGAAGTGNN I I I I IANCTGNANTNTTTTTNNCATTCCTCCTGAATGTANATTNNGTNCTATTTAACTGACTATTGGCGTCN

NAATCTTGTCNTGTGTNTNAACCCTCCCCANNCATCCCCANCTCCCCNACNTNCCCTCCNCCCCTCCNCCCTCTCTCTCNN

CTCCCCTCCNNGNNCNCNCCCCNCNCATCTTCNTNNACNNGNGNCTNCCCNCCNNNTNTNCTNCCCNNTGCNCTCTCTAN

ANNNGGGGNCCCCCTNAATTCCNTATTANCNTGNCCCCCCN

BGH sequence: n.d.

Running clone number: # 51

ER transmembrane protein

ACCESS No .: J03297.1 G 193094 Definition: Mouse ERp99 mRNA encoding an endoplasmic reticulum transmembrane protein.

LOCUS: MUSERPX 2759 bp mRNA ROD 12-JUN-1993 3 remark:

Effect:

Convention for the evaluation of the phenotypic 'cell death effect':

Clone # 65

Clone # 66

Clone # 67

Clone # 68

Clone # 70

BGH sequence: TTTTTTTTTTNTNGATATTAGNTANGTTTTATTATTTNTTATCTNTATGAGGAAGGGGTATCCCAGACAGGGAGACTGNTGAG

GNNACAATCCTAGAGAGANTGTCNGAGAAGACTGANAGCGACCCTGACTCANCCGTGGTACTGGGGTGCAGTTNGTGTAT

GANAAGTGTGGAANAGGGAAGGGTACTGTCCCTGATACTCCCTCTGGGCTCTGCAAAAGGGAGTAGGGAGCATGAACACG

GCTGATCTGATCCTGTGNGCTANAGATGCCATGTGCGCTGCCTCTGCTCTTGATAGGCTGGNCCTCANCAGCCACAGTCA

NAANTNCGANCTCTTCACAACANCNACNCTCANACTNNAAGATCTTCCCAAAGCCTNCANGTCTTGNCTNNGNNNTGCNGA

TNNGAGGGACCCTGCAAGGAGCCCCTGNTCNAGGANCTGGAGGNTGTNCTNCAGGGTCNGGCCCTGGGTCTCAGGAAGT

AGAGCGNACAGTANGCCTGCTNCAATGGNGAGGCTGCNAAAGATGACNATGGGAATCGNCTGGTGGACTGTTCTAGTAGC

ATCACAANGGGTGTGATGATGCCACCGACCCTGTAGAAGATGCTNACCAACCCCATGCCTGTTTGCCTGAτNATGGATGGT

NGANGAGCTNAGCTAGTGNACACATANGAGATGGTGAANGCCGCAACCGAGNCAANATTTCNCNATCATAGGCAGNANAN

AGGCNCACCGNGGGANGAACTCCTGGGATGNAAAATATAATGCCGNCCATGANCCCGNCTGGGACNGCANTCNACAGNTT

GTTTTCNNA

Running clone number: UI-14 ACCESS No .: NM_004256 Definition: Homo sapiens organic cationic transporter-like 3 (ORCTL3) mRNA. LOCUS:

Effect: - / ++

Clone # 72

Clone # 73

Clone # 74

Clone # 75 T7 sequence GCCGCCAGTGTGCTGGAAAGNGAGACTCGGCGATACTGCACTTCCTCAGAGCTTGCTGCCCACTACTGCAAGAAGTACAA

CATCCAGTACCAGGAGAGCTTCTATGCCTGGAGCACCCCAGGCGTGGGCAAGTTNGTGACTTCCATGGCTGCCTCAGGGG

GCATCTATCTCACCCTGCTGTTCCTCATTGAGACCAACCTGCTGNGGCGACTGAGAACCTTCATCTGTGCCTTCCGGAGGA

GGNGGACTCTGGCAGAACTGCAGAACCGGACATCAGAGCTGCCCGAGGACCANGATGTAGCTGANGAGAGGAGCCGAAT

CCτGGTCCCTAGCTTGGACTCCATGCTCGACACACCACTGATTATCAACGAGCTCTCCAAGGNGTATGACCAGCGAGCACC

GCTCCTTGCCGNGGACAGGATCTCCCTTGCGGTCCAGAAAGGGGAGTGCTTCGGCCTGTTGGGTTTCAATGGAGCTGGAA

AAACCACAACATTCAAAATGCTGACTGGGGAGGAGACCATCACCTCAGGGGACGCCTTTGTTGGTGGTTACAGCATCAGTT

CTGACATTGGGAAGGTGCGGCAGCGGATGGGCTACTGCCCCCAGTTTGATGCACTGCTTGATCACATGACTGGCAGGGAG

ATGCTGGTTATGTATGCACGGCTCCGAGGCATCCCANAGCGGCTCATCAATGCCTGTGTGGAGAATACTCTGCGGGGTCT

GCTGCTGGAACCCGCACGCCAACAAACTAGTCAAGACTTACAGTGGTGGTAACAAACGCAGCTAAGCACTGGCATTGCCT

CATTGGAAAGCCTGCNGTTATCTTCTGGA

BGH sequence: 1 1 I 1 I 1 I ICATGTCCCCTTATCCTTTATTTTTNATANAATCACAATATGTTTAAGNGTATCCTCAANANATGACACTACTCAGC

CCTGNANNGGTCCCTGACAATCCTGNGGCCAGNGACTTNTTGAAGGCACANAGCTAGGAACAGAGGATGNGCTTCAAGTC

TGGCCACAGCTCTCTCTTGNGCTGCCGATGCTCTGNGGGATGACATCTGGCTCAGGATCAGATCAGCCGGGTCCACTGTC

CATACTGGAAGGAACATTGACATCCAAGGATGCCTNTGCAGTTGCATTGGGAGCCAACGGTCTCAGCACCTAGTCATAGCC

AGTATCTTCCCTTCATGAAGGNATTTGCAACTTTGCTGCTCATTCCTGGCAGCCTGGAAGTCCTCATTGCTGCTNAACCCTG

TNGGCTTTCTTCCCCCCCCCCCNAGGGGAGCTTAGNGTCATATGTAAACATAGACAGGTCCCTGGGGNCTCTCTGGATAA

NCACTGGAAAGAAGCTCAATCCGCCAGGCTAGCTGCAGAACCAGCTGGGACAGTTCCCCATCCAAAGACTAAGGCCTGTC

TGTGTGCACAGCAGGATGGGGNCTGGGACTGNGGTCCAGANACCTGACTTCAGGAGTGGAAACCTGCATGCTGACTCCT

GCCTGCAGGCACTGACAGACACACATGGTCCACTTTGNGCATATCACCTTGANTGGTTTTCATGNCCATCATAGAAAAGAG

TGATTAAAAATGANNGAAAGGAGAAAACTGGGAGGGAAAGGACCCACGGGNTAAGCNCGAAATNGCCCCTTNCACGCCCN

GGGG

Running clone number: UI-25 ACCESS No .: NM_001089 definition. Homo sapiens ATP-binding cassette, sub-family A (ABC1), member 3 (ABCA3) mRNA LOCUS:

Effect:

Clone # 76

T7 sequence CGNCACCCCCTTGGTACCGAGCTCGGATCCCTAGTAACGGCCGCCAGTGTGCTGGAAAGGATCATCCGTCTCGCCTCCAG

TTCTTCACCGGCTGAGCTGGGTACGGACATCTCAGTGACCACGAGCTAACATAGCACGTGAAACTGACCTCAGGAAGAAC

GAAGCCTGGAGACCCGGAGTGCGACAGCGACCTCCGGACTGAAGTGGAAGAAGCGTAGCGAGGCCTTCGTGTCCTCGCT

GTGGGGGGCGGGGCTTGTTGTGCAGGCGGGGCTCACGTGGTACTTTGGTACTTTGGATGACTGAAGGGAAGCTTGGTAC

CCGCCTGGTCACTACCCGGAAGTCCAGCCGGAGTAGCACTACCCTGGAGTCCACCCGGCTGTGAAGCGGNGGTGCTCTT

AGGGGTGCTTGACGCCCTCAπGTTAGAAACTGATGGGTACTGTTGGCCGATTCCTAGACCCGCTTTTGACCTACGTGATA

AATGTGTTTCCGGAACTCTGCCAAAAAGTGTTCTGGAGGTTTTTGTTTGTTTCGGAGCCAAGGCCTCCCTCGTAGCTCTTAA

TGGCCTGAAACACTCTTTGTAGCCTAGACTGGCATTGAAGTCAAGTTAGCCCTGCCTTTGCCTCCCTGGGCTCTGGGATTA

CCCTGCCTTTCTTGATTATTTCTATTTGAACAAATATGAACAAACTATGTGGCCACCACAAA

BGH sequence: TT I I I I I l I I l IGTGGNGGCCACATAGTTTGTTCATATTTGTTCAAATAGAAATAATCAAGAAAGGCAGGGTAATCCCAGAGC

CCANGGAGGCAAAGGCAGGGCTAACTTGACTTCAATGCCAGTCTAGGCTACAAAGAGTGTTTNAGGCCATTAAGAGCTACG

AGGGAGGCCTTGGCTCCGAMCAMCAAAAACCTTCAGAACACTTTTTGGCAGAGTTCCGGAAACACATTTATCACGTAGG

TCAAAAGCGGGTCTAGGAATCGGCCAACAGTACCCATCAGTTTCTAACAATGAGGGCGTNAAGCACCCCTAAGAGCACCAC

CGCTTCACAGCCGGGTGGACTCCAGGGTAGTGCTACTCCGGCTGGACTTCCGGGTAGTGACCAGGCGGGTACCAAGCTT

CCCTTCAGTCATCCAAAGTACCAAAGTACCACGTGAGCCCCGCCTGCACAACAAGCCCCGCCCCCCACAGCGAGGACACG

AAGGCCTCGCTACGCTTCTTCCACTTCAGTCCGGAGGTCGCTGTCGCACTCCGGGTCTCCAGGCTTCGTTCTTCCTGAGG

TCAGTTTCACGTGCTATGTTAGCTCGNGGNCACTGAGAT

Current clone number: UI-26

ACCESS No .: ESTs AI315969, AI930239, AI666299, AV141103

Definition: mouse ESTs

LOCUS:

Effect: - / +

Clone # 77

T7 sequence TTCACCCCGCTTGGTACCGAGCTCGGATCCCTAGTAACGGCCGCCAGTGTGCTGGAAAGATGGAGCCTGCGTTTCACAGA

GGGGATCTCCTTTTCCTCACGAACCGAGTTGAAGATCCTATACGCGTGGGGGAGATCGTTGTTTTCAGGATAGAAGGAAGA

GAGATTCCTATAGTGCACCGAGTCCTGAAGATCCATGAAAAGCAAGATGGGCATATCAAGTTTTTAACCAAAGGAGATAATA

ATGCTGTTGATGACCGAGGTCTCTATAAACAAGGACAACACTGGCTGGAGAAGAAAGATGTTGTGGGGAGAGCAAGAGGG

TTTGTTCCTTACATTGGAATTGTGACGATCCTCATGAATGACTATCCTAAATTT GTATGCAGTACTGTTTCTGCTCGGTTT

ATΓTGTGCTGGTCCATCGTGAGTAAGAAGTCGGACTCCCTGTTCCTAGGAAGCTGCTGTGCTTGTTGTTACTGAATGTTGG

AGTAGAΤCCTGATCTGTGATΤGCGGATTTTCGGAGGACACACACGTTGGCACTTCTTGGTAGCCCTGGTTTGCATTGCTTT

GTGTTTCCACACCAGAGGCTGTGTGGGCGGGTGCATGTGCACCGTGGAGTGCACACAAGGGGACTGTCAATCACAGGGT

TTCATATGTTGTCATTGTCACTCTTTCACATTTTTGTCATCAGTGMTTTTTTATATTAA GGTTGAGCCAA ^

TTTGTATTTTGAAGCCNAGCTTCACTTTAA GTGCCTACAGAGTTCTGTAAATGAAAACACAGCTCTGCATGAGTTCAAACC

TGNCGGTCCTTCTTACAGTAGGAATGGCNCATANTGAGGCGGGCATAAGTCTTACTTT

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H P CD p c α o z cυ

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Clone # 79

Clone # 80

-4

Clone # 81

Clone # 82

Clone # 83

Clone # 84 T7 sequence TNTGACGCCGTTCTAGCATTNAGGTGACACTATAG TAGGGCCCTCTAGATGCATGCTCGAGCGGCCGCCCTTTTTTTT

I I I I I I I I I CCGTTATAATAGCCATCTTTATTTGTAAAAATCCAGATATAAAACGTAATCTTTCAGTCTTTCCAGGTTTTCCT

TTTTACAAAAACAAAAAGGCACGTATAAACCTTGCCCGCTGTCGTCCCCGTACACGGNGTTTCTCAGGCAGCCCTCCCCC

CGCCCCGCCCCCCGTTACAGCTACATGCTTCATTCCAGGACGTCTGCATCCCCACATGCTTTGGNGCTTTCCTACCAGG

AGAGTTCCGAGCTCCAAGACTTGAAGTACACAAAGAGGGGGTAGGGGTGGGTGCAGNGTGTGGCACAATGTTCCACGG

GTGCAGGGCAGNGGGCTAGTAGTAGGTCTCCTTCTCCACCCAGCCGCCAGGGCGCCGCCTGATAATGAGCTTCCGCAC

TCGTCATACACAAAGATGAGAAGGGAGTAGGGGAAGGCACAGAACCACCATGTAGGTTTGAGGGGATACATCCTAAAGG

TGCCCCCATCCCGGGGCAGTAGGATAAGAAAGCAGCAAGGGCTGTCTCTTCAAAGAGGCCAAATATCAAGATCTTGGTC

CATTCCCTGCTGGA

BGH sequence: TTCCANCCCCCCTTGGACCGAGCTCGGATCCTCTAGTAACGTCCGCCAGTGTGCTGGAAAGGGGTTGCCATGGGGATTG

TGGCTCTGATGTGTCCAAGCAAGCTGCTGACATGATCCTTCTGGATGACAACTTTGCCTCCATTGTGACTGGAGTAGAGG

AGGTCGTCTGATTTTTGATAACTTGAAGAAATCCATCGCTTACACCCTAACCAGTAACATTCCGGAAATCACCCCCTTCTT

TATTTATTATTGCAAACATTCCACTGCCCCTGGGGACTGTGACCATCCTCTGCATTGACTTGGGCACTGACATGGTTCCTG

CATCTCCCTGGCCTACGAGCAAGCTGAGAGCGACATCATGAAGAGGCAGCCCAGAAACCCCAAAACGGACAAACTTGTG

ACGAGCGTCTGATCAGCATGGCCTATGGACAGATCGGTATGATCCAGGCCCTGGGAGGCTTCTTCACTTACTTTGNGATT

TGGCTGAGAACGGTTTCCTGCCCTTTCACCTGTTGGGCATCCGAGAGACCTGGGATGACCGCTGGGTCAACGATGTGGA

GACAGCTACGGGCAGCAGTGGACCTACGAGCAGAGGAAGATCGTGGAGTTCACCTGCCATACAGCGTTCTTTGTCAGTA

TGTGGTAGNGCAGTGGGCCGACTTGGTCATCTGC

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3V9N999W39V3V99V39NV9VN9V9V999 99913J.1V1311V319199V19V9131911V3931V3911VVV99V91VVVV1VV133111331913V33313V339199VV 991333V9JJL33113391911991391399933913913933913913913933913913913933913913913913913913913913913913913913913913913913913913913933913913913913923

9V9V1391VW133V31919V1V1311V1333999V9V131V391V39V9313933993999W ^^ VV3JL1V9VVV1V3V91VV13VVV1VW1911VVVVVV9V13V1V11313W 1119VW9W91W9V3W.LU3331V311W1.UV1V99 9.Lα999131V991113V191933V91V91V1399139119939919 \ Λ 3VVVV9919V3 l I I IV3391HW9W99993

31999V99V91V13V339V39319331991933133V93V31199V99331V19V9V339991191V3V939V39V331 13W9V3931333993913199VW991391919V33933993W19V11331V993139V933V199J93L933V199JJ

98 # uo |

BGH sequence: I I I I I I I I I I I I I I 1 I I I I I GAATCTTTATGTCATTAGTTTATTTACAATTTTTTCTAGTATAAGAGTTCAAGAGTTTAATCCAAT

TTCAGATCACCTCTTA CTTTCTTCATTCTGTTAAAGGGATGAATTAAATATCCTTATTTTTTAAGTAGCTGGTGTCTTACTA

A GAAAGGTGCGGCA CCCAGATCCAMGTACACGGTCATCATAGCCAGCAACCGCCACTTGTTTTCCACTGAAAATGG

CAAATTCTTCCCCGGACCCTCCTCATAGNGGCTGCGACGGACCACGGAGGTCGTGAACCTCCGGATACTCTGGCCCAACA

TGTCGCTGCTGGAAGTTCTGCGAGGGCCGCAGACCTTTCCAGCACACTGGCGGCCGTTACTAGTGGATCCGAGCTCGGT

CCAAGCTTGGGTCTCCCTATAGNGAGTCGTATTAATTTCGATAAGCCAGTAAGCAGNGGGTTCTCTAGTTAGCCAGAGAGC

TCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTC TGGGGCGGAGTTGTTACGACATTTTGGAAA

GTCCCGTTGATT

Current clone number: UI-61 ACCESS No .: NM_007749 Definition: Mus musculus cytochrome c oxidase subunit Vllc (Cox7c), mRNA LOCUS:

Effect: - / ++ (moderately fast)

Clone # 86

4

©

CVI

Beach Plus / Plus

Clone # 122 T7 sequence ATGCACCACCAGTCTGTTCTGCACAGCGGCTACTTTCACCCACTGCTTCGGAGCTGGCAGACTGCTGCCTCCACCGTCA

Table 2

Clone 0001-T7 sequence

AGCTCATGTTGGACAGTCGTGTGAGGAGCTATGGAGCACAGCAGTAATCGCCCAGAGGACTTCCCGCTTAACGTGTTCTC TGTCACTCCGTACACACCCAGTACCGCCGACATCCAGGTGTCCGACGACGACAÄGGCAGGGGCCACTTTGCTΓTTCTCAG GCATCTTTCTAGGACTGGTGGGGATCACTTTCACTGTCATGGGCTGGATCAAATACCÄAGGTGTCTCCCACTTTGTANAT GGACCCAGCTCCTCGGACCCATCCTTCTGTCGGTCGGAGTGACATTCATCCTGATCGCTGTGTGCAAATTCAAAAYGCTA TCCTGCCAGTTGTGCTCAGATAACGAGGAGAGGGTCCCGGGACTCCGGACCAGGACTTCCSGAGGACAGTCGTTCGTTTT CACTGGCATCAATCAGCCCATCACCTTCCACGGGGCCACCGTGGTGCAGTATATCCCTCCTCCTTACGGTTCTCAGGAGC CCCTGGGAATGAACGCCACCTACCTGCAACCCATGATGAATCCTTGCGGTCTCATACCTCCTAGTGGAGCAGCGGCTGCC GCACCAAGTCCCCCTCAGTACTACACCATCTACCCTCAAGACAATGCTGCGTTCGTNGAGAGNTGAGGGCTTCTCTCCTT TCGTGGGCACTGGATATGACAGGCCCGACTCTGATGCTGACCAGCTAGAAGGGACGGAGTTGGAAGAGGAGGACTGCGTA TGTTTCTCTYCTCCACCGTATGAGGAGATATACGCTCTACCTCGCTAGAGACTGCAATGCTAAGGGGACGGACATTTAAG CCCCTGNGATGTGATACTTGGAGAGTTTATCGCTGAGTTCTTCAGAAGTTAGGTGTCAAAGCAGCTNAGGAGATCTTACA GATGTCATTNAAGGNGGGAAAGAAGTGCCCNGAGACTGCTAAATTAAGCTGCCCTGGTTAAATTCCCCTCTGCTCTGGTT TTGAATTCTCTCAGCTAAGAAACCC TCTGCAGCTGGAGAGTCGCTCTGAGATAGAGAGATTTNGGAGCCCACGCAGNGCC

T

Clone 0001 BGH sequence

ATAGACATGTCTAGTTTTTTATTACTAGTTATCATCCAAGTGAAATGTCCCTGAGGCATAATATGAATCACAATAATAAA

CCAAGATTGTTTTGCTGATATCCTTGAAAAACCTGGACTCTTCTGACAGAGAGTAAAAGCAATCCCACATATAAGCACAG

TACCAAAACCTTCAAGACCTGACAATTCCTGTATCTCTCTTGGGGGCAGCCACCTTTACCTTGAGTGGCCTTTGATCTTT

GTAAAACTGTTTTCTTTCTAATTCTTTGGACACCTCCTAAAGTCTGCAGCTTTGGGCTGNGGTTGGTGACACCGTTCAGA

CTTAGGAAGCTGAAGGCTACCAGCTTTTCTTTGTTCAAATGAAGGTGÄGAGC ACGCCCGGGGGCGGAGGKAGRGGGAGA

CGGGATGACTGATCATCTCCATGATTCATGACTACAAAGCGACACGGCAGNATGGCTCTGNGGAACAGATCAGAGGCATA

CCTGTGTTCCATAAGCNACTCAGCTGTACCTGCGTTCTGTAAGCCACTCGGCTGAGCTAGTTCGNGCCTGGGAATCACAG

CCTGGGGGNGGGCAGAGGGAGCAGGCGCTNACCNATGTACATTACGCCTTGAACTTCANCCTTGCAAAGGAAAACNATCA NGAGCAGGGGTCACTNTAACTCGGNGGACAGCACATGGNGACATTCATTATCCTCCTGNTACCTCGTCCACAGTAGGATC GGCCACCCAACACTATTCTAACATGGGGCACTGTTTCATCCNACTCTACTTATTCATTTATTTCTTAATGGATTTTATTT CTTTAACAAACTCTTNAAGATCCAAAGGCTTCCAGNGAGACCANATAANAGTCNAAGTTGTCTAANATAAGTNACTCTGC TGCGGAAGGTTCAGNGCCGTGCAGGGAAATTTCTACCTGAGCCTGCTCTCTTCCCTGCTTGCTTGCCAGCCTCCCTTTNA TCANTCTNAGAGCTGCCATGGCTGCCCGGATTTAAΆACTAΆNACAATCTTTNAAAACTAAGATNTCCTTTAAATGGATAT TCCGCATGG

Clone 0002 contig

CGCCGCCTGAGCGCCCGGCCCGACCCCGCCATGGGGTGCTGCTATAGCAGCGAAAACGAGGACTCGGACCAGGATCGGGA

GGAGAGGAAGCTGCTGCTGGACCCCAGTAGCACCCCTACCAAAGCCCTCAATGGAGCCGAGCCCAACTACCATAGCCTAC

CTTCAGCTCGCACAGATGAGCAGGCCCTGCTTTCCTCCATCCTTGCCAAGACAGCTAGCAACATCATTGATGTGTCTGCC

GCAGACTCCCAGGGCATGGAACAGCATGAGTACATGGACCGGGCAAGGCAGTACAGTACCCGCTTGGCTGTGCTTAGCAG

CAGTCTGACCCATTGGAAGAAGCTGCCACCGTTGCCATCTCTCACCAGCCAGCCCCÄCCAAGTGCTGGCCAGTGAGCCTA

TCCCCTTCTCTGACTTGCAGCAGGTCTCCAGGATAGCTGCGTATGCCTATAGTGCACTTTCTCAGATCCGCGTGGATGCG AAAGAAGAGCTGGTTGTACAGTTTGGGATCCCATGAAGAGAGGGGCCCTAGGACAGCTCTTCCCTCGTCTTCACCCCGTC TCCACCCCACCTCTTCTGGCCCCCAGCCTCACTGTGGCTCTCTACAGTACCTAACCTGCTACTAΆTCACGGAGAAGAATG TGGAGGGAAAGAACAAGGCTGGAGGCCGGAGCAAGTGAGGACTAAGCAAGGGAAGGGAGGACCGATTGCCATCGGCCTTC ATGCTCTGGTTAGGGTGAGGTTGGGGCCAAGAGGACAGGGCCTGGCAGATCTTCAGTCATTGGGAAGATGGAGATACCNC TGTAGGGGTGACACCGGGAGACCTAGGAGATCCCTTCCTGCCCTCTTTCTCTTGGCCTCCGATTCACTCCTGTCCCCTTC CCTGACTTGGTGCTCACAGGCACCTCACTGGGGATTATGACCAGGGTCTAGACGAGCTTGAGTCTGAATTGAGTTTGTAT TTCTAGCACCCTGGGTTTTTACATGTTTGCTCTTTTTGTTTTTGTTTGTCACCCCTCGATAAAGAAAGTATATTCATTTG ÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁ

Clone 0003-T7 sequence

AGGTTCCTGTCGTTTATGCTGCGCGCCGCGAGCATCTTCGTGAAGGACGCTGTGCTCTASTCTGGCTTCACGCTGGATGA GGCCGAGCGCCTCACGGAGGAAGAGTTGCATATCATCGCGCAGGTGCCGCCTCCACCCGCCGCTGCCGCCGTTGGCTACG CTGGCTGCCGTGTGGCTGTGACCTTCTTCCTCTACTTCCTGGCTACCAACTACTACTGGATTCTGGTGGAGGGACTGTAC TTACACAGCCTCATCTTCATGGCCTTTTTCTCAGAGAAGAAGTATCTGTGGGGCTTCACCAΓCTTTGGCTGGGGTCTGCC GGCTGTCTTCGTGGCTGTGTGGGTCGGTGTCAGAGCAACCTTGGCCAACACTGGGTGCTGGGACCTGAGCTCTGGGCACA AGAAGTGGATCATCCAGGTGCCCATCCTGGCATCTGTTGTGCTCAACTTCATCCTCTTTATCAACATCATCCGGGTGCTT GCCACTAAGCTTCGGGAGAC AATGCGGGCCGGTGTGACACCAGGCAGYAGTACCGGAAGCTGCTCACGTCCACGTTGGT GCYNGCGCCACTCTTTKGTGTCCACTACACCCGTCTTCATGGCCTTGCCGTACACCCGAGGTCTTAGGGACACTGGGGCA GATCCAGATGCACTATGAGATGCTCTTTAACTCCTTTCAGG

Clone 0004 contig

AGGCGCTGCCTACCAGAGCGCAGCATGACGGCCΆTCGGCGCGCAGGCCCACAAGCTGTTGGGCCTTAAGAGGCCCCACCG GTCTTTCTTTGAGTCCTTCATCCGGACACTCATCATCGTGTGCACTGCCCTGGCTGTGGTCCTTTCTTCAGTCTCCATCT GCGATGGCCACTGGCTCCTAGTGGAGGATCATCTCTTTGGGCTGTGGTACTTCTGCACCATCGGCAACCACAGTGAACCA CACTGTCTGAGAGACCTGAGCCAGGCCCATATGCCCGGGCTGGCTGTAGGCATGGGCCTAGCACGCAGTGTGGCCGCCAT GGCAGTGGTGGCTGCCATCTTCGGCTTGGAGATGCTCATTGTGTCCCAGGTGTGTGAAGATGTCCGCTCACGGCGCAÄGT GGGCCATCGGTTCCTACCTCCTCCTGGTTGCCTTTATCCTCTCCTCTGGGGGCCTCCTCACCTTCATCATCCTGCTCAAG AATCAGATCAACCTCCTGGGCTTCACCCTGATGTTCTGGTGTGAATTCACTGCCTCCTTCCTCTTCTTCCTCAATGCCGC CAGCGGCCTTCACATCAACAGCCTCACTCAGCCCTGGGACCCTCCAGCAGGGACCCTGGCTTACAGAAAGCGAGGTTATG ATGGGACTTCTCTGATATAGGACCTCTGGCTGAGATTGAATAGGACAΆCCAATGACCTTGACACTGCCTCTTΆGGCACTT AGCTCTAGCAATGCCCTGGAAGTCTCTTCAGCTGAGCTCCAGGGCAAAGGCAGAAGGGTGCCTCTGTACGACGGCACAGT GAGCTGGATAGGTTAGTCATGCTATTAAAATCTCAATTTGAAAGTAAAAAAAAAAAAAAA

Clone 0005 contig

AGCAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGC CATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACTT TGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTCACGGCAAGAΆGGTCGCCGATGCTCTGGCCAATGCTGCAGGCCACC TCGATGACCTGCCCGGTGCCCTGTCTGCTCTGAGCGACCTGCATGCCCACAAGCTGCGTGTGGATCCCGTCAACTTCAAG CTCCTGAGCCACTGCCTGCTGGTGACCTTGGCTAGCCACCACCCTGCCGATTTCACCCCCGCGGTGCATGCCTCTCTGGA CAAATTCCTTGCCTCTGTGAGCACCGTGCTGACCTCCAAGTACCGTAAGCTGCCTTNTGCGGGGCTTGCCTTCTGGCCAT GCCCTTCTTCTCTCCTTGCACCTGTACCTCTTTGGTTTTGAATAAAGCCTGAGTAGGAAGAAAAAAAAAAAAAAA

Clone 0006-T7 sequence

AGGGGCTTCGGACCCGGAAGTGGCGCCTTGGGCTCCCGGCGGCGCCGCGGGGATGGCGGGAGCCGGAGCTGGTGCAGGAG CTCGGGGCGGCGCGCCGGCCGGAGTCGAGGCCCGCGCTCGGGACCCGCCACCCGCGCACCGCGCGCACCCTCGCCATCCT CGGCCCGCGGCTCAGCCGTCGGCGCGCAGGATGGACGGCGGCCCGGGCGCCCCGGGCTCCGGGGACAACGCCCCGACCAC CGAGGCGCTGTTCGTGGCGCTGGGCGCGGGCGTGACGGCTCTCAGTCACCCGCTGCTCTACGTGAAGCTGCTGATCCAGG TGGGTCATGAGCCGATGCCCCCCACCCTTGGGACCAATGTGCTGGGGAGGAAGGTCCTCTACCTGCCGAGCTTCTWCAAC CTATGCCAAGTACATTGTGCAGGTGGATGGGAAGATAGGGCTCTTCCGGGGCCTGAGCCCCCGCCTTATGTCCAACGCCT TGTCCACTGTGACCCGCGGCAGCATGAAGAAGGTTTTCCCTCCAGATGAGATGGAGCAGGTTTCCAAACAAGGACGACAT GAAGACCTCACTCAAGAAAGTTGTGAAGGAGACATCGTATGAGATGATGATGCAGTGTGTATCGCGAATGCTGGCCCATC CCTTACACGTGATCTCGATGCGATGCATGGTGCAGTTTGTGGGACGGGAGGCCAΆGTACAGTGGTGTGCTGAGTTCTATT GGGAAGATCTTCAAGGAAGAGGGGCTGCTGGGATTCTTCGTTGGCTTAATCCCTCACCTCCTGGGCGATGTGGTTTTCTT GTGGGGCTGTAACCTGCTGGCCCACTTCATCAATGCCTACTTGGTGGACGACAGCTTTAGCCAGGCCCTGGCCATCCGGA GCTACACCAAGTTTGTGATGGGGATTGCAGTGAGCATGCTGACCTACCCCTTCCTGCTCGTTGGAGATCTCATGGCAGTG AACAACTGTGGGCTGCGGGCTGGACTC CCTCCGAATTCCCCTGAGTTCAAGTC

Clone 0006 BGH sequence

GAGCAGTGTTGGGGTGGCATCCCCCTTCCTGCCTAGAGGTACTGGAGTCCATCTTGTACTCAGGCAGAGGCAGGCTGCAG AGGCAAACGTCACTCAGTGGCAAGGCTTCCCTGCACCTCTAGCCCAGCTCATCCTGCCAGTCAGCCAGAAGCACCCCCGC CCCCCACTTCCTGCTTTGTAAATTGGGCGCCATCACACCTGGGCCATGGGAGGCTGGCGCTATGTTCCCAACACTAATTT TCTTATACAAGGGTGGTGCCTTCTCCTGAATAGGAAATCATGTTCTCCTCAGACCATCCCCTCATCTGCTTGTCTGTGCT GGTGACGCCAGGTGTGAGGGTTCAGTCACTGTGCTGGGTGCGAATACGCACAGGTTACATAGGCCGACÄTCTAGTCCTCC CCTCGTGGTAAGATAGACCCATCTCCTCGAATAΑATGTATTGGTGGTGATTTGGAAAAAAAAAAAAÄAAAAA

Clone 0007 contig

AGCTCCGCCCCTGCTACTGGACCATGGARACTGTGGCCCAGTAGAGACCTTAGTGTGAGGCTTTCAGGGGCGGCGGCCAT

GGAGGCCGTGCTGAACGAGCTGGTGTCTGTGGAGGATCTGAAGAATTTTGAAAGGAAATTTCAGTCTGAGCAGGCAGCTG

GTTCTGTGTCCAAGAGCACGCAATTTGAATATGCCTGGTGCCTGGTTCGAAGCAAATACAATGAGGACATCCGCAGAGGC

ATCGTGCTGCTGGAGGAGCTGTTGCCCAAAGGGAGCAAAGAGGAACAGCGGGACTATGTCTTCTACCTGGCCGTGGGCAA

CTACCGGCTCAAGGAATATGAAAAGGCTCTAAAGTATGTGCGAGGGCTGTTGCAGACTGAGCCCCAGAACAACCAGGCCA

AGGAGCTGGAACGCCTGATTGATAAGGCCATGAAGAAAGATGGACTGGTAGGCATGGCCATCGTTGGTGGCATGGCCCTG

GGCGTGGCAGGCCTGGCTGGACTCATTGGACTGGCTGTCTCCAAGTCCAAATCCTGAAGGCAGCCTCACCTGCTCTCTGC

CCCGGGACGCCTAGGAGCCTGGGGGACACTGGAAGAGGGGCCTGTCCATCCTCACCATCGCCTTCCCTTTTCTCCTGCAC

CCCTGTAGTCTACCTCTACAGTCTCCATGACCCCCAGCCTYTTAGCCCCTGCACCTGTCGTTTAACCCTGTCATGCTTTG

CAATGAGTGTAAATAAAATTGGGCCGTGGCTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA Clone 0008 contig

AGATTGACTTGGGCACTGACATGGTTCCTGCCATCTCCCTGGCCTACGAGCAAGCTGAGAGCGACATCATGÄAGAGGCAG CCCAGAAACCCCAAAACGGACAAACTTGTGAACGAGCGTCTGATCAGCATGGCCTATGGACAGATCGGTATGATCCAGGC CCTGGGAGGCTTCTTCACTTACTTTGTGATTCTGGCTGAGAACGGTTTCCTGCCCTTTCACCTGTTGGGCATCCGAGAGA CCTGGGATGACCGCTGGGTCAACGATGTGGAGGACAGCTACGGGCAGCAGTGGACCTACGAGCAGAGGAAGATCGTGGAG TCACCTGCCATACAGCGTTCTTTGTCAGTATTGTGGTAGTGCAGTGGGCCGACTTGGTCATCTGCAAGACCAGAAGGAA TTCTGTCTTCCAGCAGGGAATGAAGAACAAGATCTTGATATTTGGCCTCTTTGAAGAGACAGCCCTTGCTGCTTTCTTAT CCTACTGCCCCGGGATGGGGGCAGCCCTTAGGATGTATCCCCTCAAACCTACATGGTGGKTCTGTGCCTTYCCCTACTCC CTTCTYATCTTTGTGTATGACGAGGTGCGGAAGCTYATTATYAGGCGGSGCCCTGGCGGCTGGGTGGAGAAGGAGACCTA CTACTAGCCCYACTGCCCTGSACGCCGKGGAACATTGTGCCACACACTGCACCCACCCCWACCCCCTCTWYSYSYMCTTY AAGTYTTGGAGCTCGGRACTCTWCCCTSRWAGGRWWKYWSSRARGCMTKTGGGRATKYWKΆCSYCYTGGRATGRAGSAWR TAGCTGYAMCSSRRRRCSGKKYGKKGGGGAGGRYTGCCTGAGAAACNCCGTGTMCKKGGACGACAKCGGRMAAGRATTK TACGTGCCTTTTTGTTTTTGTAAAAAAGGAAAACCTGGAAAGACTGAAAGATTACGTTTTATATCTGGATTTTTACAAAT AAAGATGGCTATTATAACGG

Clone 0009-T7 sequence

AGCCGGAGGCCGAGCCCAGTCGCCAGCTCCTGCTCTGCTCCTCTCCCGCCTGCCGCCGCGCTGCACGCCTCGAGCACTCC CTCGGCCCCGGCGGGGACCGGGGACCCCGCAGCTACCGCCATGCTGCCAGTGCTCTACACCGGCCTGGCGGGGCTGCTGC TGCTGCCTCTGCTGCTCACCTGCTGCTGCCCCTACCTCCTCCAAGATGTGCGGTACTTCCTGCGGCTGGCCAACATGGCC CGGCGGGTGCGCAGCTACCGGCAGCGGCGACCCGTGCGTACCATCCTGCGGGCCTTCCTGGAACAAGCGCGCAAGACCCC ACACAAGCCCTTCCTGCTGTTCCGAGACGAGACGCTCACCTACGCCCAGGTGGACCGGCGCAGCAACCAAGTGGCGCGGG CGCTGCACGATCAACTGGGCCTACGACAGGGGGATTGCGTAGCCCTCTTCATGGGCAATGAGCCGGCCTACGTGTGGATC TGGCTGGGACTGCTCAAACTGGGCTGTCCCATGGCGTGCCTCAACTACAACATTCGTGCCAAGTCTCTGCTGCACTGCTT TCAATGCTGCGGGGCGAAGGTGCTGCTGGCCTCCCCAGATCTACAAGAAGCTGTGGAGGAGGTTCTTCCAACCCTGAAAA ANGATGCCGTGTNCCGTCTTTTACGTAAGCAGAACTTCTAACACAAATGGTGTGGACACAATACTGGACAAAAGTAGA

Clone 0009 BGH sequence

TTTTTTTTTTTTTTTAGTGTTAATATAGTTTATTATGTCTTTAAAAAAATAAGGCCCTCTCTCCAAGAAGCTTAGTTTGC

AAGGACAAATGGCAGGTGCACATTGAAAAATAATTGTTTCTAAATCTTTTTACTTGCAAAGGTTCAGGTGTAATTTAAAA

AAAAAACAAACAAACTATCCTTTTAATGAATAATTTCCTAAAAATAAAATCGCACCTTATAGCCTTCAATCAAGTTAAAG

TTGGATTCTACGTATGAAGTGGCTCTGCGAGGTCTATCGAGTTTCTTTCTGGAAATGTCATGAGCTAAACCACCAGGGAA

TATTCAGAGCTTCAGAGTTTTATCAATTATGGCATTATAAATGTTCTCAGTCATGGGCACAAATGTTTTCTCTGCATCAT

CCATGAAATACAAGGTATCTTTGATGACTGTGGGATTGAAGCCCTCTTCCATCAGGGTCACTTTGCGGTGTTTAAAAGTC

CCAGTGATCTCAATGGTATCTTGTATCCTCAGGAACCGAGGCCTCGCGTAACTGGGCAGGTACTCCGCGATGTGTTGAAA

GAGTTTCTTTCCATTGAACTCGTAGTTTTCTTTGATCTTGA

Clone 0010 contig

AGCGCGGGAGGGGCATGGCGGGCATGGCGCTGGCGCGAGCATGGAAGCAGATGTCCTGGTTCTACTACCAGTACCTGCTG

GTCACTGCGCTCTACATGCTGGAGCCCTGGGAGCGAACCGTGTTCAATTCGATGCTGGTTTCCGTGGTGGGGATGGCCCT

GTACACTGGCTACGTCTTCATGCCCCAGCACATCATGGCTATTCTGCATTACTTTGAAATTGTACAGTGACGAAGATGTG

ACCAGGATCCAGAGGTTCCTGGGGAAGATCTGCCTTGTGAAGTTGGAATGAGACCTCATCAGATGTAAGATGTGCTACGG

ATGTCCACGTGACCAACCTTATAAATACAAAGACTTTAAAAAAAACTTCATGAGTAGAACAGGAAAAATCATCCTGGCTC

ATGTGTTG GTTCTTTCTTTTTGATTTTAACAGAGGCTCTTATATAGTAGCTTTTATCTATTTTAACATTGTAGTCATTT

GTACTTTGATATCAGTATTTTCTTAACCTTTGTGACTGTTTCAATATTATCCAGTGAAAGCTTTTCTTAATGTAACTTTG

AGTACATCTCAATTGCCTTCTATTTTTAAAACCTAAGGTCATTAGTTGGGCTTTACTGTTCTTGCTATCATATGGCATAT

ACATCTGCCTGGATATATTTCTACTCTTGACCAAAGTTTTGTAAGAAACAATACAGAATTTGGGGACGGGAGGGAGGATA

CTTTGAGAACTACTTACAAAAGATTTATCTGCTTGCTTGAACTCAGGAGTACGGTTTTAGCTCCCTAGACTCTTAACAGC

TTTTGCTCTAAAACTATTAAAGTGTTTCTTAGTAATGAAAAAGTAAAGATCTTGCTAACATTAATACAAGGAACATTTCN

CCTTTTAGATATTAAATTCTTATGTGGACTTATTTCCAACCAACTTTGATGATTCCTGTGATTGGTGATTGGTGGCCGGC

TTGTATGACATATGCTGAGATACACTGTGGAGCTTTTCATAGAAGGCTTATCAGATCCTCTGAGGCAGTATTGACCCTGT

ATACGCTCAAGAGTTAATTTGATGTTGTAACAGGCGTTGTGAAATGCATTTGCACGTTGTGATAAAGACMCAATGAGATG

AATTGTGCAGAACCATAACCAAAAGAAATGTAAACTTGCTTAAAAATCCTTTCACTCTTTGTATTTTTTTTAAAACGGTT

TTTATTCCTTAGATGTAAAATGACTACTTTTTTTTGATGTAAACTCATTAAATTCAAAGAAAAATGTAAAAAAAAAAAAA

ÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁ

Clone OOU-Contig

AGCGAGTTTGCAGACTTCTTGTGCGCAGCTAGCCGCCTNAGGTGTTNGAACCATGAÄTCTTTTACTCCTTTNGGCTGTCC TCTGCTTGGGAACAGCCTTAGCTACTCCAAAATTTGATNAAACCTTTAGTGCANAGTGGCACCAGTGGAAGTCNACGCAC AGAAGACTGTATGGCACGAATGAGGAANAGTGGAGGANAGCGATATGGGAGAAGAACATGAGAATGATCCAGCTACACAA CGGGGAATACAGCAACGGGCAGNACGGCTTTTCCATGGAGATGAACGCCTTTGGTGACATGACCAATGAGGAATTCAGGC AGGNGGNGAATGGCTATCGCCACCAGAAGCACAAGAAGGGGAGGCTTTTTCAGGAACCGCTGATGCTTAAGATCCCCAAG TCTGNGGACTGGAGAGAAAAGGGTTGNGTGACTCCTGTGAAGAACCAGGGCCAGNGCGGGTCTNGNNGGGCGTTTAGCGC ATCGGGTTGCCTAGAAGGACAGATGTTCCTTAAGACCGGCAAACTGATCTNACTGAGTNAACAGAACCTTGTGGACTGTT CTCACGCTCAAGGCAATCAGGGCTGTAACGGAGGCCTGATGGATTTTGCTTTCCAGTACATTAAGGAAAATGGAGGTCTG NACTCGGAGGAGTCTTACCCCTATGAAGCAAAGGACNGG TCTTGTAAATACAGAGCCGAGTTCSCTGTGGCTAATGACA CAGGGTTCCGTGGATATCCCTYAGCMAGGAGAAAGCCCTCATGAAGGCTGTGCGACTGTGGGGCCTATTTCTGTTGCTAT GGACGCAAGCCATCCGTCTCTCCAGTTCTATAGTTCAGGCATCTACTATNAA.CCCNACTGTAGCAGCAAGATCCTCGACC ATGGGGTTCTGTTGNTNGGCTATGGCTATNAAGGANCAGATTCAAATAAGAATAAATATTGGCTTGTCAAGANCAGCTGG GGAAGTGAATGGGGTATGGAAGGCTACATCNAAATAGCCNAAGACCGGGNCNACCTCTGTGGACTTGCCNCCGCGNCCAN CTATCCTGTCNTNAATTNATNGGTAGCGNTAATGAGGACTTATGGNCTCTATGTCCNAAGGAATTCAGCTTAAAACTGAC CNAACCCTTATTNANTCNAACCNTGGTNCTTNAATCATTNAGGATCCAANTCATNATTTNAATTCTGTTGCCATTTTTAC ATGGGTTAAATGTTACCNCTACTTAAA ACTCCTNTTATAAACANCTTTATAATATTGAAAACTTAGTGCTTAATTCTNAG TCTNNAATATTTNTTTTATATAAAGGTTGTATAAAACTTTCTTTACCTCTTAAAAATAAATTTTAGCTCAGTGTGTGTGT AA

Clone 0012 contig

GAGCGCCGCCGGGTCGGCCAGCGAGGCCTCAGAGGTTCCAGACAACGTGGGAGACTGGCTCCGCGGCG CTTCCGCTTCG

CCACCGATCGAAACGACTTCCGGAGGAACTTGATCCTYAATTTGGGACTCTTTGCTGCGGGAGTCTGGCTGGCCAGGAAC

TTGAGTGACATTGATTTGATGGCCCCTCAGCCAGGGGTGTAGCCAGAGAATGGAACTCCTGTGTATTCAGACTTTCCAAA

GACAGCCTACTGTCTGTGACCACAAGATCCTACCTGAGTGGCAGCTGAAGTTGACTCCCTCTCCKTGCCTGAACCCCCCC

CC CTGCCCCCCCATCCCCCAGTGTCGGCTRAGATGTTGCCTCTGCACGGTTCTGTGTGCAGTTCCCNACTTTCTGCAGA

AGATGGTCCTTGCCCTTGTCCTNAAGAGTAATAATGGTTCTTGAAAAAGATTTCAAATAAAGCCTGCNCAT

Clone 0014-T7 sequence

AAAGGAAAAÄCACAGCTCAGCAGATCCAGGCACTAAAGAGAGCTAGCTGCAAGCAGGAGCAGTCAAGAATCTGTGGTCAG

AAGTACTGGAGTGGGCCAGCAGGGCCAGCTTTTTCTACCATGGCAGCCCAAGGCTACGGCTACTATCGCACTGTCATATT

TGCGGCCATGTTTGGAGGCTACAGCCTGTACTATTTCAACCGCAAAACCTTCTCCTTTGTCATGCCCTCCTTGGTGGATG

AGATCGCTCTGGACAAGGACGATTTGGGGCTCATCACAAGCAGCCAGTCGGCAGCCTACGCCATCAGCAAGTTTGTGAGC

GGGGTTCTGTCAGATCAGATGAGCGCCCGCTGGCTCTTCTCCTCTGGGCTGCTCCTGGTTGGTCTGGTCAACGTAGTCTT

CTYATGGAGCTCCACAGTGTCAGCCTTWGCTGCTCTTTGGTTTCTTAATGGTCTGGCACAGGGGCTGGGCTGGCCCCCCT

GTGGGAAGATCCTGAGGAAGTGGTTTGAGCCATCCCAGTTTGGCACTTGGTGGGCTGTGTTGTCAACCAGCATGAACCTG

GCTGGAAGTTTGGGACCTATCTTGGCAACGATCCTCGCCCAGAGCTACAGCTGGCGCAGCACACTGGCCCTGTCTG

Clone 0014 BGH sequence

TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGDAACAAATCTAGCTTTATTGATCTATACAA

GKCGGTTTGCCCTCTATATTCTTCCCTTTTGKCCCAGGGTATTCATGAGAGGGGGGGÄCYCCTYTYCTCCCTTAGGGACT

GRGGTATTGGCAACAATTTTGCCCCCTGCCAAGAAGCCTTGGGGTACAGCAGAAACCACAGGCCATTAATACTCACTAGG

AGATCAGGACCTAGGAGCAAGAAGAAGGGTATAGGAGACACTCTGAATTCAGGAGTTGCCTGTCTGCCAGAAGGKAGAAA

CAGAAGCTGA GGAGTCTAGAACCAAACATCATCATTTTAAATAGAGCATGGGAAGGGAGT

Clone 0015-T7 sequence

AGCGGAACTCAAGATGGCTGCGCTCTTGCTGAGTGCTGCTCCTTTGGGAACCACAGCTAAGGAGGAGATGGAGCGGTTCT

GGAAGAAGAACACGAGTTCAAACCGTCCTCTGTCTCCCCATTTGACTATCTACAAATGGTCTCTTCCTATGGCACTGTCC

GTTTGCCACCGAGGCTCTGGAATAGCCTTGAGTGGAGGGGTCTCTCTTTTTGGCCTGTCGGCACTGGTGCTTCCTGGGAA

CTTTGAGTCGTATTTGATGTTTGTGAAGTCCCTGTGTTTGGGGCCAACACTGATCTACTCGGCTAAGTTTGTGCTTGTCT

TCCCGCTCATGTACCACTCACTGAATGGGATCCGACACTTGCTATGGGACCTAGGAAAAGGCCTGGCAATACCCCAGGTC

TGGCTGTCTGGAGTGGCGGTCGTGGTTCTTGCTGTGTTGTCCT

Clone 0015 BGH sequence

CTTYTTTTTTTTTTTTTTTTTTTTAAAAKGAGTAGTACTTTAATCGTTTCCAAAGAAGATGGTACATGAATTTAGATTAG

AAAATATGACATTAAAGGTGTATTTTCCTAGAAATGAAAAACAGAACAGAAACTTAATACAATTTTTTTTGATCTTCCTC

ATACATCAAAAGCTGTGTATCTGTTCCCAGGCTTGAGCTCCAAGCTCCCAGGCTGCTCTTTATCTCCCTCTAGCTGAGCA

GGACAATCCCTCCTCCTCCACCTCTCTCCTCTCAGTCATGAGGTGTGGGTGCTAAAGGCCCTAAGCTGACCAATGGACAG

AAGTGTCCTTTAAGCCAGCAGCCCACAGGCAGTGGCACTCGGGGCGGTCACAGAACTGGCATTGTTTMYACTGTCTCAGC

AGAAAGCAGCGGAAAGCTGACCCTCAGCACAAATCAGTCAAATGTGGAACCGGAGCAAGGGCTCCCAGGCCCAGGGAGGA

TGACCCTTTCGTACTACAACAGGTCTTTAAGCCGCTCTACTGCACTCCAAGGGGATTGAAAGCATGTGGCATCTAAACAA

ATCAGGAGAACCTGGGTGGT Clone 0016 contig

GAGGGGAACAAGATGGCGGCGCCAAAGGGGAAGCTTTGGGTCCAGGCCCAACTGGGGCTCCCGCCGCTGCTGCTGTTGAC

TATGGCGCTGGCCGGAGGCTCGGGGACTGCAGCGGCCGAAGCCTTTGACTCGGTCCTGGGAGACACAGCGTCCTGTCACC

GGGCCTGTCAGCTGACCTACCCCTTGCACACCTACCCGAAGGAAGAGGAGTTATACGCATGCCAGAGAGGCTGCAGGCTG

TTTTCAATTTGCCAGTTTGTGGATGATGGGCTTGATTTAAATCGGACCAAGCTGGAATGTGAATCTGCGTGCACAGAAGC

ATATTCCCAACCTGATGAGCAGTATGCTTGTCATCTTGGCTGCCAGGATCAGTTGCCATTTGCTGAACTGAGACAAGAAC

AACTCATGTCCCTGATGCCAAGAATGCATCTCCTCTTCCCTCTGACTCTGGTGAGGTCGTTCTGGAGTGACATGATGGAC

TCTGCACAGAGCTTCATAACCTCTTCATGGACTTTTTATCTTCAAGCCGATGACGGAAAAATAGTTATATTCCAGTCTAA

GCCAGAAATTCAGTATGCACCGCAGTTGGAGCAGGAGCCTACAAACTTGAGAGAATCATCTTTAAGCAAAATGTCCTATC

TGCAGATGAGAAACTCACAAGCACACAGGAACTACCTTGAAGAGGAAGAAAGCGATGGCTTTTTAAGATGTCTATCTCTT

AACTCTGGATGGATTTTAACCACAACCCTTGTCCTCTCGGTGATGGTGTTGCTCTGGATCTGTTGTGCAGCTGTTGCTAC

AGCTGTAGAACAGTATGTTCCCCCTGAGAAGCTGAGTATCTATGGTGACTTGGAATTTATGAATGAACAAAAGCTGAGCA

GATACCCAGCTCCTTCTCTTGTGATTGTTAGGTCTCAGACTGAAGAACATGAGGAGGCAGGGCCCCTGCCCACCAAGGTG

AACCTTGCTCACTCAGAAATCTAAGCTTTTTAAAAGAGTCGTGGACACATAAACTTCCATTCCTCATAGAGCTTTTTAAG

ATGGTTTCATTGGACATAGGCCTTAAGAAATCACTATAAAATGCAAATAAAGTTACCAAACTCTGTGAAGACTTTATTTG

CTGTGACTTTACCTGTATTTTTCTAGTCATTTAAGATGGACATTGGGTTGTATTTTTATTTTACTAATATCTGTAGCTAC

TTAGTTAGTTGCATTGGTTTTGGTTTTTTTCCTCTCTTCGCCAAATTCTATGAGCTGATCATTGTGGCCCCGCCCCTGCC

ATGCCCCCCGTCAGTCATCTCACTTAATAACCGAAACCTTAGGGTGTGATGCTTCTGCCCGGAAATGGCCTCCAAACTGT

CCTCTGGATTATAGCAGAAATGTTATTTAATGACWCTACATTTTCAGTTGTATTGAATTGAAATCATTAAAATCTATTTG

AATAATTATGTTCTGAAAAAAAAAAAAAAAAAAAA

Clone 0017-T7 sequence

AAAGGTACGAAGCTAGGGAAGATATTCGCGTGGCTAAATCTGCACGTGGAAGGAGCATTAACTTGGCCCTTTCTTATAGA

GGACGGCAGGCCTTGAAAGCCATTGGTCTGGAAGATCAGATCGTTTCCAAAGGTGTGCCCATGAAAGCCAGAATGATCCA

CTCTCTTTCTGGAAAGAAGTCTGCAATTCCCTATGGGAACAAGTCACAGTATATCCTTTCAATAAGCAGAGAAAACTTAA

ACAAGGACCTGCTGACTGCCGWGGAGTCCTATGCCAATGCGAAGGTGCACTTTGGCCACAAGCTGTCGAAATGCATTCCG

GAGGAAGGGGTACTCACAGWGCTCGGACCTGACAAGGTTCCCCGAGATGTCACATGTGACCTTGTTGTAGGGTGTGATGG

AGCCTATTCAACTGTCAGAGCCCACCTCATGAAGAAGCCCCGCTTTGATTACACTCAGCAATATATCCCTCATGGATACA

TGGAGTTGACAATTCCACCTAAGAATGGGGAGTACGCCATGGAACCTAACTGTCTTCACATTTGGCCTAGAAATGCCTAT

ATGATGATCGCCCTTCCGAÄCATGGACAAATCTTTCACATGCACCTTGNTCATGCCCTTTGAGGAGTTTGAAAGACTTCC

AACGCGCAGCGATGTGCTGGACTTCTTCCAGAAGAACTTTNCAGATGCTATCCCTCTGATGGGAGAGCAAGCCCTCATGA

GAGA

Clone 0018 contig

CCCTTTTNNCCCCAATTGGGCCGTTAGGCGTTTCCGGTGGAAAGGTCCTANNTTAAGCAGANGCCNCTTCGGGGCTAACC

TANAAGNNCCCCNCTTGGNTTNCCNGGCCTTNTCGGAAATTTNATTTCGGACCNCCCCTTTAAGGGAGNCCCCAAGCTTG

GGTCCCAAACCTCGGNTTCCCCTAGTTCCGNGCGGCCAATTGTGCTGAAAGCGRCTGCAAGTGAAGCCCTCTGTTACCT

GTGTCGATCAAGACCTCAACCCCAGAGGAACTTCGTCATCAACATGACTTGCAGGTTTTGCTGGCAGCTTCCTGAAACA

GACTACGAGTGTTCAAATTCCACCACCTGCATGACCGTGGCTTGCCCTCGGCAGCGCTATTTCGCCAACTGCACCGTGCG

TGACCACATTCACTGCCTGGGCAACCGGACTTTCCCTAAGCTGCTGTACTGCAACTGGACAGGTGGCTACAAGTGGTCGA

CAGCCCTGGCTCTCAGCATCACCCTTGGGGGGTTTGGAGCCGATCGCTTCTACCTGGGCCAGTGGCGAGAAGGCCTCGGC

AAGCTCTTCAGCTTTGGCGGCCTGGGAATATGGACCCTAATCGATGTCTTGCTGATTGGAGTTGGCTATGTGGGACCAGC

GGATGGCTCTTTGTACATTTAGCCGAGGTTATGTGCTTCAGAGAGCAGTGTAGAGTCCTGTGTGTGGAGATGGATGCGGT

GTGAGAGGGGGATTTATGTTTTTAATGTACAGCATCTGTACTTGGCTTGCCTTGATGAAGGTAAAATAAAGAAATCGAAC

MCTGAAAAAAAAAAAAAAAAAAAAAAA

Clone 0019-T7 sequence

CCTGGTAGGAGCCGCAGTGTGTGGCCATGCCTCAGATAGGTTTGGGCGCAGAAGGGTGCTGACCTGGAGCTATCTTCTGG

TGTCCGTGTCCGGCACAGYAGCTGCCTTCATGCCCACCTTCCCCCTCTACTGCCTGTT SGTTTCCTGCTGGCCTCTGCA

GTGGCAGGTGTCATGATGAACACAGCCAGTCTCTTGATGGAGTGGACATCAGCCCAGGGTAGCCCTTTGGTGATGACCTT

GAACGCCTTGGGCTTCAGCTTTGGGCAGGTCCTGACAGGTTCTGTGGCCTATGGTGTGCGCAGCTGGAGGATGCTACAGC

TGGCTGTCTCTGCCCCCTTCTTCCTCTTCTTTGTTTATTCATGGTGGCTACCAGAATCGGCACGCTGGCTCATCACGGTA

GGCAAGCTGGACCAGGGCCTGCAGGAACTACAGAGGGTAGCTGCTGTCAACAGGAGGAAGGCAGAGGGAGACACGTTGAC

CATGGAGGTGCTGCGGTCAGCTATGGAGGAGGAACCAAGCAGGGACAAAGCTGGTGCCAGTCTGGGCACTCTACTCCACA

CACCTGGGCTGCGCCACCGAACCATCATCTCCATGCTGTGCTGGTTTGCCTTTGGCTTCACCTTCTACGGCCTGGCCCTT

GACCTGCAAGCCCTAGGAAGCAATATCTTCCTGCTCCAGGCACTCATCGGGATTGTGGACTTCCCGGTGAAGACAGGCAG

CCTGCTGCTGATCAGCCGCTTGGGCCGGCGCCTCTGCCAGGTCAGCTTCCTGGTGCTCCCCGGACTCTGCATCCTGTCCA

ACATACTGGTGCCCCATGGGATGGGGGTCCTTCGCTCGGCCCTGGCTGTGCTGGGGCTGGGCTGCCTGGGGGGCGCCTTC

ACCTGCATCACCATCTTTTCCAGTGAGCTCTTTCCCCACTGNGATCAGGGATGACTGCAGNGGGCC Clone 0019 BGH sequence

TTTTTTTTTTTTTTTTTTTTYTCTTTAAAAATTATTTATTTGKGTGTATCACCGGGGGCGGGTATGTGTCCATGCGGAGC TCAGAGGACAACTTTGTGAAGTCTGTTGTCACATGAGTTTCAGAAÄTTTAGGCCCGGAGGCAGGTGTCTTTACCCGCCGT CGCCAGCCCTGCCCTTTCCTCCTCCTCCTCCTCTTCCTCCTCCTCCTCCTCT CCTCCTCC

Clone 0020 contig

GAAAGGGGACGGAGACCTTCAGCGTGGAATCTATATCCAAGAATGGAATCTGTCTGGAGATGGGCCCACAGCCTCAGGGC

GTGCTGCGGGCCGACCTGTTCTCCCGGATGCGAGCTCTGGTGGCATCCATTCTGGACTTCATCGAGCTCTTCAACCAAGG

CATGGACTTACCCGCCTTTGAGATGGATATCTACAGGAACTTGGGCAGTGTGGACTTCCCACGCACTGCGGATGGTGACC

TGGCTGGCACTGTGCACCCTCAACTGCAGGACCATGACTTTGAGCCACTGAGGCCTGGTGAACCCATCTTCAAGCTTTTC

AGCGGAGAAGACGTACTGTATGAGGGGGACTCCATTGTGTACCCTGTGTTCATTAATGAGGCTGCCTATTATGAGAAGCA

CGTGGCATTCCTGAAGTCTGAGAAGATCAGGGTCACAGTGCCTGCCCTGCTGAGGTTGACCCCCCGCTCCACCCAGACTC

CCTAACCCAAATCATACCTGGCCTGATCTCAGTTTTCCTATCTGAACAATGGGTCCCAAGCCCAGGGTCCCCTATCTCAC

TCTGGACACCATATCCCTTTCCAGACAACTGATCTCATTATATAAAATAGACTGTGCCCCAAAAAAAAAAAAAAA

Clone 0022-T7 sequence

AGCGGACGTTTGTGCCGGGACATGGCCGCTGCGGATGCCGGGTCCTTGAGCTGAGCGCTTGCTGCCGGAGCCAACCTCTG

CCGTCAACCGTCCGCGCGGGCTGGGCCCAGGCGCCGGGACGGCCAAGATCCGCCGAGGAAGCTGAGGCAGCTATAGAACG

CCGCCGCGGCGGGCGCATGGCGTCCATCTTGCTCAGGAGCTGCCGGGGCCGGGGGCCTGCCCGCCTCGCGCCACCTCGGG

CCGCCTCCCCGCGGGGTAGTCTGAGGGATCGAGCTTGTCTCAGCTGTACCAGGACCCTGGGGTTGACGAGCCGTGAGAGT

GTTCTGTCTCGTTGCTGTACTCCAGCCCACCCTGTGTACCTCTGCTTCAAAGGTGAGCCCCTCAGCTGTTGGACTCAGAG

GCCTGAGTGCCAGGGCACCGCAGCAAGAACAACATGGACACCTGCCTCTGCAAGGCTGGTGGTTACGGGACCTCAGTACC

TTCCTGTGCGCGGCTGGCACTCATCATCTYCGCTAGGAGAGGACTCTGTGATAGAGAAGTCCCTTAAGTCCTTAAAAGAC

AAGAATAAGAAGCTGGAGGAGGGCGGCCCCGTGTACAGCCCGGCCGCGCAGGTGGCTGGTGAGGAAATCCCTNGGGCAGA

AGGTACTGGATGAGCTGAGGCACTACTACCATGGCTTTCCGCCTGCTCTGGGATNGACACCAAAGANCGCTTG

Clone 0022 BGH sequence

TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATTATGGAAGTTTCTGATTTATTCCAGACAAAATATTAGATTTGCC

ACTAAGAATCACCTCAAAGCAATCACTCTTGNGTGTCATGCCCCTGNGGCTGGCACGGCATGATGAAGGCAGACCTGNGG

GCCAGAAGCTGGCAGCTCTAATTCTTCACTTCTGCGGCCTCTTTCTCAGCCTTCTCCTTGGCCTTCTCTTTCTCTTCTAT

CTTTTCTTCTTTCTCTAGNGNGGCCACAATTTCAGCTACCTGGGNGGNAGAGATCTGAACATCTTCTTTGTTCACCAAGT

CGATTACCTTGACAAGGTCATCGATGTTGATATTGCCGNCCTTATTATCATCCAGGGCTGAGGTCAAACTGATGAGCTTG

AGCTCTGGAATGNGCTTGATTTGCTTCATGGCGCTGATGAGCTCAGTGATGCTGATGACACTCTCCCCTGTWGGAGNGCT

CTGGCTGGGGCCCAGCTTGCCATCCTGWTGAGAGGWCTCCAGCTGTGTGATCAGGCCATCGATCTGCCCGATCATCTGCT

GCACTCGCTTTGACAGCCTCTTGCTCGCTGCARA TCTTCTATGNACTTTCTCCTCACCCGTCTNTTGAAAGKTCCTTCT

TTGA CTCCTGTAAGTCCTCACNTGTAGGCCTGGACGCCCTCTTTTGAGMRSRSNTCCAGGCTTCCTCCTTTCTCCCTTG

GTCAGGGACTTCTTCTGCTNCTGCAGCTTGGAACAGGCGTACTGAGGATGTNAA

Clone 0023-T7 sequence

ATGGACCGCTTCGTGTGGACCAGCGGCCTCCTGGAGATCAACGAGACCCTGGTTATCCAGCAGCGCGGGGTGCGCGTCTA

CGACGGCGAGGAGAAGATAAAATTTGATGCCGGGACTCTTCTTCTTAGTACACACCGGCTGATTTGGAGAGACCAGAAGA

ATAATGAGTGCTGCATGGCCATTCCCCTGTCTCAGATTGTGTTCATCGAGGAGCAGGCAGCTGGAATCGGGAAGAGCGCC

AAAATCGTGGTTCACCTGCACCCAGCTCCTTCTAACAAAGAGCCCGGTCCGTTCCAGAGCAGTAAGAACTCCTACATCAG

ACTCTCCTTCAAAGAACATGGCCAGATTGAGTTTTACAGGCGTTTATCAGAAGAAATGACACAGAGAAGATGGGAGACTG

TGCCAGTTTCCCAGTCACTACAAACAAATAAAGGACCCCAGCCAGGAAGAGTAÄGAGCTGTTGGAATTGTAGGTATTGAA

AGGAAACTTGAAGAAAAAAGAAAAGAAACCGATAAAAACATTTCTGAGGCCTTTGAAGACCTGAGCAAGCTGATGATTAA

GGCTAAGGAAATGGTGGAGTTATCCAAATCAATCGCTAATAAGATTAAAGAGAAGCAAGGCGATGTCACAGAGGATGAGA

CCATCAGGTTTAAGTCGTACCTGCTGAGTATGGGAATAGCCAACCCAGTCACCAGAGAAACCTATGGCTCCGGCACACAG

TACCACATGCAGCTGGCCAAACAGGCTGGCTGGAATATTGCAGGCACCTTTGGAGGAGCGTGGGGGAATAATGNCCCTCA

CAGAGGNG

Clone 0024-T7 sequence

AAAGTGGGGATCCTGGWGACAGTTCTGGTGCAGAGTTCCAGTACCTCTACATCCATCATTGTCAGCATGGTCTCCTCCGG

CTTGTTGGAGGTGAGCTCCGCCATTCCGATCATCATGGGCTCCAACATCGGAACCTCTGTCACCAACACCATTGTGGCCC

TGATGCAGGCAGGGGACAGGACTGACTTCAGGCGGGCTTTTGCAGGGGCGACCGTGCATGACTGTTTTAACTGGCTGTCT

GTTCTGGTCCTACTGCCCCTGGAGGCTGCCACGGGCTACCTACACCATGTCACCGGGCTTGAGGTTGCTTCCTTCAACAT

CCGAGGTGGCCGTGATGCCCCCGACCTTCTCAAAGTCATCACAGAGCCCTTCACAAGACTCATCATCCAGCTGGACAAGT

CTGTGATCACCAGCATTGCCGTGGGGGATGAGTCCCTGAGGAATCACAGTCTCATTCGGATTTGGTGTCACCCAGACACA ACAGAGGCTTCCACTTCTATGTCCAGGGTAGAGGCCATCGGCAGCCTTGCAAACACCACCATGGAGAAATGCAACCATAT CTTCGTGGATACGGGGCTGCCCGACCTGGCCCGTGGGGCTCATTCTGCTGGCGGGCTCCCTGGTGGTTCTCTGCACTTGT CTTATCCTCCTTGNGAAGATGCTCAACTCCCTGCTCAAGGGCCAAGTGGCCAATGTCATCCAGAAGGTCATCAACACAGA CT

Clone 0025-T7 sequence

AGGGCGCCAGCTGAAGACGCGGGACTTAAAGCGCGTAGCCAGAACCCAGGCACCAGTGTGTCCATTGTCCAGAACTCATC

TGAAAAACTGCCACAGGAATTGCTTCTCTGCTCCAGGCTGGTCACTGAACAGGTTGCTCCAGGACCTGCAGAATGGGGGC

AGGCTGTGTCAAAGTCACCAAGTATTTCCTCTTCCTCTTCAACTTGCTGTTCTTTATCCTGGGTGCTGAGATCCTGGGCT

TCGGGGWGTGGATTCTTGCAGACAAGAACAGCTTCATTTCCGTCCTACAAACCTCATCCAGCTCGCTGCAGGTGGGGGCT

TACGTCTTCATCGG GTGGGCGCCATCACCATAGTGATGGGCTTCCTGGGCTGTATCGG GCTGTCAATGAGGTCCGCTG

CTTGCTGGGTCTGTACTTTGTCTTCCTTCTGCTGATCCTCATCGCACAGGTGACCGTAGGGGTCCTCTTCTACTTCAACG

CTGACAAGCTGAAGAAGGAGATGGGGAACACAGTGATGGACATCÄTTCGCAACTACACTGCCAATGCCACCAGTAGCCGC

GAGGAGGCCTGGGACTACGTGCAGGCGCAGGTCAAGTGCTGTGGCTGNGTCAGCCACTACAACTGNACAGAGAACGAGGA

GCTCATGGGCTTTACCAAGACCACTTACCCATGCTCCTGCGAGAAGGATCAAGGNAAGAGGACAACCAGCTCATTGTGAA

GAAAGGATTCTTGCGAGGCTGATAACAGCACCTGTGAGCGAAAACAACCCCTGAGGATT

Clone 0026-T7 sequence

AGGAGCCCTCGAAAGCGACATGGCGGTTCTCTTAAAGCTGGGCGTTCTCTGCAGTGGCCAAGGAGCTCGAGCTCTCCTAC

TCCGAAGCCGGGTGGTCAGACCCGCTTATGTGTCAGCATTTCTCCAGGACCAGCCTACCCAAGGACGG GTGGTACCCAG

CACATTCACCTGTCACCAAGCCACCACTCTGGTTYCAAGGCTGCATCTCTCCACTGGACCAGTGAGAGGG WGTCAGTGT

TCTGCTCTTGGGGCTGATCCCTGCTGGGTACTTGAATCCCTGCTCTGTGGTGGACTACTCTCTGGCTTCAGCCCTCACCC

TGCACAGTCACTGGGGCCTTGGACAAGTGGTTACCGACTACGTTCATGGGGACACCCTGCCGAAGGCTGCCAGGGCAGGC

CTCTTGGCACTCTCAGCTTTGACCTTTGCTGGGCTTTGCTACTTCAATTACCACGATGTCGGCATCTGCAGAGCGGTTGC

CATGCTGTGGAAGCTCTGACCTGGGTGCAGCACTTTGATTGTGTGCCTCCTTGCCTCTGCTTTACCAATGCCGTTCACCT

CGCAGTGAGGGGGGATGAAGGATAAGCCCATTGGTGGGCAGAATGTCTTCTAATTACATGGGTATTTTCAGAATTATTTG

NTGAGGAAGAGGNTT

Clone 0028 BGH sequence

TTTTTTTTTTTTTTTTTCTTTTTTTAAGATAATGGTCTTTTAATTGAGTTATTGAGATGAAGAGACAGTGAAGCCCTGTT

TGCTACTTACATGAAAAGAAGATTTTAAAAAACAATCACTGCACAAAATACAAAGGGGCAGGGTATGCTGWGGCATTGAA

TTCCTCCCCACGTTTTTTCTTGACTTCTCAAGAACAAATTAAAGTCTCCACAGCAAATTTGTTCTCAAAATGCCGAATGG

TGAAACAGTTACTGGCTTCCCGCTTCTGAATACCTCTAATTGTTTCCCGGCGCTGCAGTTTGTAGGTCTCCTTGTCGTGA

CACAGTCGGTAGATGAAGAAGCCCAGGTTGTCCACGTTCTCGATGCGGTCGGTGATCACCATGTGCTCATGGATAAGGTA

GGACTGAGGCAGGTAGGTCCCGGCCTTAATGTTAATAAGGAGCTCCAGCAGGTTTTTGGGCGGCATAACGATGGAAGTGT

TCAGAGGAATCACGTAGCACTTGTCCAGGTTAAGGT

Clone 0029-T7 sequence

AAAGAGCGGCTGCTGTCGGAAGCACCGGGCGAGCTATCTGTTACAGTCCGGCCCGGGGATGGCTCGGGACGCGGAGCTGG

CGCGCAGTAGCGGGTGGCCGTGGCGG GGCTGCCGGCGCTGCTGCTGCTGCAGCTGCTGCGGTGGAGGTGCGCCCTGTGC

GCGCTCCCCTTCACCAGCAGTCGGCACCCAGGCTTTGCGGACCTGCTGTCGGAGCAGCAGCTGTTGGAGGTGCAGGACTT

GACCCTGTCTTTGCTGCAGGGCGGAGGTCTAGGGCCGCTGTCACTGCTACCTCCGGACCTGCCGGATCTGGAGCCTGAGT

GCCGGGAGCTGCTGATGGACTTCGCCAATAGCAGCGCCGAGCTGACCGCCTGTATGGTGCGCAGCGCTCGGCCCGTGCGC

CTCTGCCAGACCTGCTACCCGCTCTTCCAACAGGTCGCAATCAAGATGGACAACATCAGCCCGAAACATCGGGAATACCT

CCGAGGGCCCGCGCTGAGGCGGAAGTCTCCTGACGGCAGACAGAATGCAGATAGTTCTCATGGTCTCTGAGTTTTTCAAC

AGCACGTGGCAGGAGGCGAACTGCGCAAATTGCCTAACAAACAATGGTGAGGATTTGTCAAACAACACAGAGGACTTCCT

CAGTCTGTTTAACAAGACTTTGGCCTGCTTTGAGCATAACCTGCAGGGGCACACATACAGNCTCCTTCCACCAAAAAATT

ACTCCGAAGTGTGCAGAAACTTGTAAAGAGGCATATAAAAACCTGAGCCTNCTGTACAGTCAAATGNA

Clone 0029 BGH sequence

TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTGTTATTTCCTTTATTGGAAAGCATAAGGAAAAAACAGGTTTT

CTTGDGCACACACATAACCCTATGKGCCTMAGGATTCAGAACTATGTACATTTTTTTAATATGACCACAAGATGAAATTC

TTTGGCACATTTTCAAATATATTTCTAATGCAACCTCTAGAGAGCCAGACCCTGATCAGGAACAGAGATGGCTGGCTTGT

TAAAGGGCTCTCCAGCTTCTTAGCCAAAAGCAGGGGTTTGTACACACAGTACTGAAAGGNACCCGAGGAAGTCGCTACTC

ACAGGTTTAAATATGTCACTTCACTCACTTTGCACATGTAAATAAGNTTTACATGTACTGATGAAGATGGNTTCCAATGA

CCCTNAACCATGNGCTTCAAATCAAGACAGGAACAATGACAGCNCAATGAACCCCGGCACÄTNTAGGGGATCACAGCGNC

GNCTGATTGTCACATACCCGGGGTGACACACTCTGGGACTAAGACTAGCCTGCTNTCACACTCTGCANATGTGGNAAACA

TACAAAAAATACCCAAACACTCCTGCCTTCCTGTAGGGCAAANACAGGTTTTNAAGG Clone 0030 contig

AAAGGCGCGGAGCCTGCTGCTGCCATGGTGGCTGGTGGCTGGGTACACTATGTCTAGACTGGGGGCCCTGGGCGGCTCCC

GCGCCGGGTTGGGACTGTTACTTGGTACTGCCGCCGGCCTTGGATTCCTGTGCGTCCTTTACAGCCAGCGATGGAAACGG

ACCCAGCGCCATGGCCGGAGTCACAGTCTGCCCAACTCCCTGGACTATGCGCAGGCTTCAGAGCGTGGACGCCAGGTGAC

ACAGTTTCGGGCTATCCCAGGTGAAGCTGGAGATGCTGCCATACTGCCCAGCCTCTCACAGGAAGGGCAGGAGAAGGTGC

TGGACCGCCTGGACTTTGTGCTGACCAGTCTTATGGCGCTGCGGCGCGAGGTGGAGGAGCTTCAGAGAAGCCTGCAAGGA

CTAGCTGGGGAGATTGTCGGGGAGGTCCGCTCTCATATAGÄAGAGAACCAGAGAGTGGCCCGGCGGCGCAGGTTCCCTTT

TGCCAGAGAGAGGAGTGACTCCACGGGCTCCAGCTCTGTCTACTTCACCGCCTCCTCAGGGGCCGCACTCACAGACGCCG

AGAGCGAGGGAGGCTATACAACAGCCAACGCGGAGTCTGATTACGAGCGGGACTCCGACAAGGAGAGTGGAGATGCTGAG

GACGAAGTGAGCTGCGAGACCGTGAGGATGGGGAGGAAGGACTCTCTGGACCTGGATGTGGAGGCGGCGTCCAGTCCGTC

Clone 0031-T7 sequence

AGGCTTGCCGTCCAGGCCATGCGGCTGGWGGTCTGCATCG GGCGTTTCCCATGTTTCTGCTGAACCTTCTAGGCATGTG

GAGCTGGGTATGCAAAAAGTGCTTTCCCTACTTCCTGAAGCGGTTCGCCATGATATACAATTGGAAGATGGCGAGCCTAA

AGCGGGAGCTCTTCAGCAATCTGCAGGAGTTCGCCGGCCCCTCGGGGAAGCTAACTCTGCTGGAGGTGGGCTGCGGCACC

GGGGCCAACTTCAAGTTCTATCCCCCCGGG GCAGGGTCACTTGTATCGACCCYAACCCCAACTTTGAGAAGTTCTTGTT

CAAGAGCGTCGCAGAGAACCGGCAGCTGCAGTTCGAGCGCTTCGTGGTGGCAGCCGGGGAGGACATGCACCAGGTGACCG

ATGGCTCTGTGGACGTGGTGGTCTGCACCCTGGTGCTGTGCTCGGTGAAGAACCAGGAGAAGATTCTGCGCGAGGTGTGC

CGAGTGCTGAAGCCGGGAGGGGCTTTTTACTTCATAGACCATGTGGCAGATGAACGGTCTACCTGGAATTACTTCTGGCA

GCAGGTCCTGGCTCGTGTGTGGTTCCTTGCTTTTGACGGATGCAATCTGACCAGAGAGAGCTGGAAGGCCATAGAGCAGG

CCAACTTCTCCAAGCTGAACCTGCAGCACATNCAGGCCCCGCTCCCCTTGACCTTGGTGCGGNCCCACATCTATGGCTAT

GCTGTGAAATAGAGT

Clone 0031 BGH sequence

TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAATATAGAAACCATTTATCAAATGAATATAAATGTATT

GATCAACATTTAAAATATAACTTCTGCAAAATCATCTTGAAAAATATACATTTGTTTAGATCCATACATACAAATGCAGC

TGAAACCCTTGGGCCCACCCAGACTTGCTCTCTGTATGAACACAATGATATCCATGGTTTTGTTTCAGGACCAGKGGAAT

TTTTCTTCTTCTTCAATACAGGGTTTATTTGTGTAGCCCTGGCGGKCCTGGAACTCCATCTGTAGATCAGGCTGTACTCA

CAGAGATCCACCTACCTCTGCCACCTGAGAGCTGGGATTGAGGCTCTGCCACCAACCACTCAGGACCAGTGATATTTGAC

CAGAAGAATCCCTCCCCCCCCGSACCCCGAGTCCTTGGTAACTACTCTCGGGCATTACTTTTAGGGKCCCCTACATACTG

GACCCATTTCCCTAACTATAGNGGCCTTCTACTGCCTACGGTAATTAT

Clone 0032 contig

AAAGAAGGAACTAAACATGGGCCAGCGATGCTCTGACACCAGAGGAATTGCTTTCGAAGACGTCAGAGTGCCTAAGGAAA

ATGTGTTAATCGGTGAAGGAGCAGKTTTCAAGATCGCAATGGGTGCTTTTGATAGAACCAGACCTACAGTCGCAGCTGGC

GCTGTCGGGCTAGCCCAGAGAGCTCTGGACGAAGCCACGAAGTATGCCCTGGATAGGAAGACATTTGGAAAGCTGCTAGT

GGAGCACCAAGGAGTTTCATTTCTGCTCGCAGAAATGGCGATGAAGGTTGAACTCGCTAGGCTCAGTTACCAGAGAGCAG

CCTGGGAGGTTGACTCCGGTCGCCGGAACACTTACTATGCCTCGATTGCAAAGGCCTTTGCTGGAGACATTGCCAATCAG

CTAGCCACTGACGCCGTGCAGATTTTCGGAGGCTATGGATTCAACACAGAGTACCCTGTGGAGAAGCTGATGAGGGACGC

CAAGATCTATCAGATTTATGAAGGTACTGCACAAATTCAGAGGCTGATCATAGCTCGTGAGCACATTGAAAAGTATAAAA

ATTAACAGGAATTACTATTGAACGATGCATCACCCTCGTGTAACTAAGCTCCAAGCACTGTTGCTGCTTCAGGGGAAAAG

GGCTTTACTGTCTTCCCAAGGAAATGAGATAAAAGACGAGTTTGGATCTATGCGGCGGATTCCCATGGCGGAGGAACCTG

TCTTCAGCTCTATGGTGACCCTTTCTAGATAGGTTTGGCTTTTGGACAATGATTGGTCCTTAGCCCCGAATTGTGTTAGT

TTGCTCTTTGATCACTTAAAATGGAAAAACACCCTGGACTTTTAATGTTCATTCAAGTGACAGGAAAGGCGGCTTGTCAA

GGAAGAACTCATGATTCTAACATAAACACTGAAAATTTGTGGTAGATTGGACACGTCAGACTGTGACATAGCAGCATTTC

TGTGCTGAACTGTTAATTTTATAATTTTGATTATATTTGCTTTGTTTTGCACAAAAGAGTAAAAAGTTTATATTCATATT

CTCCCATTATAAAACTAAAACTTTTCTGGGAATCTTAGTACTGAACGAGAATTTTATTTGTCTTGTTTAAAATAATTCAA

TAAAGCTAACCTTAACTTAAAAAAAAAAAAAAAAAAAAAAAAA

Clone 0033 contig

AGCGTCGCCATCCGCCACCATGGTGAACTTCACAGTÄGATCAGATCCGTGCCATCATGGACAAGAAAGCCAACATCGATA

AGGGTGAGGTGTCTGCCCGCCAGGAGCTCAAGGCACGTGCCCGCTACCTGGCCGAAAAGTATGAGTGGGACGTTGCTGAA

GCCCGCAAGATCTGGTGCTTTGGCCCTGATGGCACTGGCCCCAACATTCTCACCGACATCACCAAGGGTGTGCAGTACCT

GAATGAGATCAAGGACAGTGTGGTGGCTGGCTTCCAGTGGGCTACTAAGGAGGGCGCTCTCTGTGAGGAAAACATGCGTG

GTGTGCGGTTTGATGTTCATGATGTGACCCTGCATGCTGATGCCATTCACCGGGGAGGTGGCCAGATCATCCCCACAGCA

CGCCGCTGCCTGTATGCCAGTGTGCTGACCGCACAGCCCCGCCTCATGGAGCCTATCTATCTGGTGGAGATCCAGTGTCC

TGAGCAAGTGGTGGGTGGCATCTACGGTGTCCTGAACAGGAAGCGTGGCCATGTGTTTGAGGAGTCCCAGGTGGCTGGTA

CCCCCATGTTTGTGGTCAAGGCATACCTGCCTGTCAATGAGTCCTTTGGCTTCACCGCTGATCTGCGATCCAACACCGGC

GGCCAGGCCTTCCCCCAGTGCGTGTTTGACCACTGGCAGATCCTGCCTGGGGATCCTTTTGACAACAGCAGCCGCCCCAG CCAAGTGGTAGNTGAGACGCGCAAGCGCAAGGGCCTGAAAGAGGGCATCCCAGCGCTGGACAACTTCCTGGACAAACTGT AGGCAGCNTGATACTGCCACATGTTGCACAGTGCCCACCCATCAGAAGACCCCTTGAGACTGTCCCACAGTGCTCCTNTA GAGGCTGCTGGGGCCACCCTGACATCACTCAGCACTCACTTGCTACCAATTCTATTTATTTCGGAATT CAAGATΆGCGG GAATCTCTCTGCAGGCTGGACTGGCAGGCTGTGGGGTGGGCGGGACMCGGCTCTTAMCATTTTCARAGGGAAACGCGCAG ATGTCCAAAAGTYTAAATAAATGCATTCAGAGGTTTTTGGGGTCCATGGCCAAGTGGAGTTCCCCCARAGGGGGAGGTGG GGTAAGTGCCTCCAGGAAGGCAGGCAGCCTGCCTTAGMCTTGCAGCCCGGCTGTGGGAATGAAYCATTGGAGTAATAAAC TMCAGTGGTTGATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAΆAAAAAAAAAAAAA

Clone 0034-T7 sequence

AAAGGCCAGCTCCTGCTCTGCTCCTCTCCCGCCTGCCGCCGCGCTGCACGCCTCGAGCACTCCCTCGGCCCCGGCGGGGA CCGGGGACCCCGCAGCTACCGCCΆTGCTGCCAGTGCTCTACACCGGCCTGGCGGGGCTGCTGCTGCTGCCTCTGCTGCTC ACCTGCTGCTGCCCCTACCTCCTCCAΆGATGTGCGGTACTTCCTGCGGCTGGCCAACATGGCCCGGCGGGTGCGCAGCTA CCGGCAGCGGCGACCCGTGCGTACCATCCTGCGGGCCTTCCTGGAACAΆGCGCGCAAGACCCCACACAAGCCCTTCCTGC TGTTCCGAGACGAGACGCTCACCTACGCCCAGGWGGACCGGCGCAGCAACCAAGTGGCGCGGGCGCTGCACGATCAACTG GGCCTACGACAGGGGGATTGCGTAGCCCTCTTCATGGGCAATGAGCCGGCCTACGTGTGGATCTGGCTGGGACTGCTCAA ACTGGGCTGTCCCATGGCGTGCCTCAACTACAACATTCGTGCCAAGTCTCTGCTGCACTGCTTTCAATGCTGCGGGGCGA AGGTGCTGCTGGCCTCCCCAGATCTACAAGAAGCTGTGGAGGAGGTTCTTCCAACCCTGAAAAAGGATGCCGTGTCCGTC TTTTACGTAAGCAGAACTTCTAACACAAATGGTGTGGACACÄATACTGGACAAAGTAGACGGAGTGTCGGCGGAACCCAC CCCGGAGTC

Clone 0034 BGH sequence

CTTTTTTTTTTTTTTTTTTTTAGTGTTAATATAGTTTATTATGTCTTTAAAAA

GTTTGCAAGGACAAATGGCAGGTGCACATTGAAAAATAATTGTTTCTAAATCTTTTTACTTGCAAAGGTTCAGGTGTAAT TTAAAAAAAAAACAAACAAACTATCCTTTTAATGAATAATTTCCTAAAAATAAAATCGCACCTTATAGCCTTCAATCAAG TTAAAGTTGGATTCTACGTATGAAGTGGCTCTGCGAGGTCTATCGAGTTTCTTTCTGGAAATGTCATGAGCTAAACCACC AGGGAATATTCAGAGCTTCAGAGTTTTATCAATTATGGCATTATAAATGTTCTCAGTCATGGGCACAAATGTTTTCTCTG CATCATCCATGAAATACAAGGTATCTTTGATGACTGTGGGATTGAAGCCCTCTTCCATCAGGGTCACTTTGCGGTGTTTA AAAGTCCCAGTGATCTCAATGGTATCTTGTATCCTCAGGAACCGAGGCCTCGCGTAACTGGGCAGGTACTCCGCGATGTG TTGAAAGAGTTTCTTTCCATTGAACTCGTAGTTTTCTTTGATCTTGAGGGAGGCCATCCCAATTCGACCCTCATGACCTG GCACAGGCACGCCATACACATTCACTTCTTCAACAAAATCTACCAGTCCCACGATGTCAGCGACCTTCTGNGGTAGCTAC GNTCTCTTCTTTTCACCGGAAAGTATCTNCAACCCTG

Clone 0036 contig

AAAAGGTGGAATCTATATCCAAGAATGGAATCTGTCTGGAGATGGGCCCRCAGCCTCAGGGCGTGCTGCGGGCCGACCTG TTCTCCCGGATGCGAGCTCTGGTGGCATCCATTCTGGACTTCATCGAGCTCTTCAACCAAGGCATGGACΓTACCCGCCTT TGAGATGGATATCTACAGGAACTTGGGCAGTGTGGACTTCCCACGCACTGCGGATGGTGACCTGGCTGGC CTGTGCACC CTCAACTGCAGGACCATGACTTTGAGCCACTGAGGCCTGGTGAACCCATCTTCAAGCTTTTCAGCGGAGAAGACGTACTG TATGAGGGGGACTCCATTGTGTACCCTGTGTTCATTAATGAGGCTGCCTATTATGAGAAGCACGTGGC TTCCTGAAGTC TGAGAAGATCAGGGTCACAGTGCCTGCCCTGCTGAGGTTGACCCCCCGCTCCACCCAGACTCCCTAACCCAAATCATACC TGGCCTGATCTCAGTTTTCCTATCTGAACAATGGGTCCCAAGCCCAGGGTCCCCTATCTCACTCTGGACACCATATCCCT TTCCAGACAACTGATCTCATTATATAAAATAGACTGTGCCCCAAAAAAAAAAAAAAAAAAAAAA

Clone 0037-T7 sequence

AGCTGCATCGTGGCGTTTCCCATGTTTCTGCTGAACCTTCTAGGCATGTGGAGCTGGGTATGCAAAAAGTGCTTTCCCTA

CTTCCTGAAGCGGTTCGCCATGATATACAATTGGAAGATGGCGAGCCTAAAGCGGGAGCTCTTCAGCAATCTGCAGGAGT

TCGCCGGCCCCTCGGGGAAGCTAACTCTGCTGGAGGTGGGCTGCGGCACCGGGGCCAACTTCAAGTTCTATCCCCCCGGG

TGCAGGGTCACTTGTATCGACCCCAACCCCAACTTTGAGAAGTTCTTGTTCAAGAGCGTCGCAGAGAACCGGCAGCTGCA

GTTCGAGCGCTTCGTGGTGGCAGCCGGGGAGGACATGCACCAGGTGACCGATGGCTCTGTGGACGTGGTGGTCTGCACCC

TGGTGCTGTGCTCGGTGAAGAACCAGGAGAAGATTCTGCGCGAGGTGTGCCGAGTGCTGAAGCCGGGAGGGGCTTTTTAC

TTCATAGACCATGTGGCAGATGAACGGTCTACCTGGAATTACTTCTGGCAGCAGGTCCTGGCTCGTGTGTGGTTCCTTGC

TTTTGACGGATGCAATCTGACCAGAGAGAGCTGGAAGGCCATAGAGCAGGCCAACTTCTCCAAGCTGAACCTGCAGCACA

TNCAGGCCCCGCTCCCCTTGACCTTGGTGCGGGCCCACATCTATGGCTATGCTGNGAA

Clone 0037 BGH sequence

TTTTTTTTTTTTTTTTTTTTTTTGGCAAATATAGAAACCATTTATCAAATGAATATAAATGTATTGATCAACATTTAAAA TATAACTTCTGCAAAATCATCTTGAAAAATATACATTTGTTTAGATCCATACATACAAATGCAGCTGAAACCCTTGGGCC CACCCAGACTTGCTCTCTGTATGAACACAATGATATCCATGGTTTTGTTTCAGGACCAGTGGAATTTTTCTTCTTCTTCA ATACAGGGTTTATTTGTGTAGCCCTGGCGGTCCTGGAACTCCATCTGTAGATCAGGCTGTACTCACAGAGATCCACCTAC CTCTGCCACCTGAGAGCTGGGATTGAGGCTCTGCCACCAACCACTCAGGACCAGTGATATTTGACCAGAAGAAT

Clone 0038-Contιg

AAAGGTGGAGCTGGGTGGTGTTTGGTGCGGTACGGCGGCCACTCAGTTGCAGCAGAGCAGGTGCCATCCTGTGGAAGAAC

CATGAAGCACTACGAGGTGGAGATTCGGGATGCAAAGACGAGGGAGAAGCTGTGCTTCCTGGACAAGGTAGAGCCTCAGG

CCACCATTTCTGAAATCAAGACCCTTTTCACCAAGACACACCCGCAGTGGTATCCTGCCCGCCAGTCCCTCCGCCTGGAC

CCCAAGGGGAAGTCCCTGAAAGATGAAGATGTCTTACAGAAGCTTCCTGTGGGCACCACAGCCACACTCTACTTCCGGGA

CCTCGGGGCCCAGATCAGCTGGGTGACGGTCTTCCTGACGGAGTATGCCGGGCCCCTTTTCATCTACCTGCTCTTCTACT

TCCGGGTACCCTTCATTTATGGCCGCAAATACGACTTTACGTCCAGTCGGCATACGGTGGTGCACCTCGCCTGCATGTGC

CACTCGTTCCACTACATCAAGCGCCTGCTGGAGACTCTCTTCGTGCACCGATTCTCTCACGGAACCATGCCTTTGCGAAA

CATCTTCAAAAACTGCACCTACTATTGGGGCTTTGCTGCATGGATGGCTTATTACATCAACCACCCTCTCTACACACCCC

CTACCTATGGAGTTCAGCAGGTTAAGCTGGCACTGGCCGTTTTTGTGATCTGCCAGCTTGGGAACTTCTCCATCCACATG

GCTCTTCGGGACCTTCGGCCTGCTGGGTCGAAAACCAGGAAGATCCCATACCCCACCAAGAACCCCTTCACCTGGCTGKT

CCTGTTGGTGTCCTGTCCCAACTACACTTATGAGGTGGGCTCCTGGATTGGCTTTGCSATCTTGACTCAGTGTGTCCCAG

TGGCCCTCTTCTCCCTGGTGGGCTTCACCCAGATGACTATCTGGGCCAAGGGCAAACACCGCAGCTACCTGAAGGAGTTC

CGCGACTACCCGCCCCTGCGCATGCCCATTATTCCCTTCCTGCTCTGAGCAGCTCCTCACGGCTCTGCCCAGTAATACTC

TCCACCCCTCACTGCCCCTGTCCTGATGTGTGGCTGGCCATGGCTCTCCAGCAGCAACAATAAAACCTGCTTACCCAAAA

AAAAAAAAAAAAAAAA

Clone 0039 T7 sequence

AAAGCGCTGGAGCTACAGCCGTTACTGCCGCCGCCGCCGCCGCCGCCGCCGAGGCGTTTGATCGTTGGCAATGTCAGGCT TTGATAACTTAAACAGCGGTTTCTACCAGACGAGTTACAGCATCGACGAGCAATCTCAGCAGTCCTATGACTATGGAGGA AGTGGAGGACCCTACAGCAAGCAGTATGCTGGCTGWGACTACTCGCAGCAAGGCCGATTTGTCCCTCCAGACATGATGCA GCCACAGCAGACATACACTGGGCAGATTTACCAGCCAACTCAGGCCTATCCTCCAACAACACCTCAGCCATTCTATGGAG ACAGCTTTGAGGAGGAGCCCCCTCTGTTAGAΆGAGTTGGGTATCAATTTTGACCACATTTGGCAAAAAACACTAACGG G CTACACCCCCCTGAGGGCAGCTGACGGCAGCATCATGAATGAKNACGGACTTGGCAGGGCCAGTGGTGTTCTGCCTTGCC TTTGGGGCCACACTGTTACTGGCTGGCAAAATCCAGTTTGGCTACGTATACGGGATCAGTGCGATTGGATGTTTAGGAAT GNTTTGNT

Clone 0040-T7 sequence

CTCTCCTTTGCCCTGCGCACGGGTACACGGCACGGTGTGGACACTCACCTGTTCAGTGTGGAGTCGCCGCAAGAGCTGGC

AGCCTGGACCCGACÄGTTGG GGATGGCTGTCATCGGSCTGCTGAAGGCGTACAAGAAGTGTCTACAGCCTGCACGTGGA

ACGGCCGTCCCTGCAGCCTGTCTGTGCACATCGACAAGGGCTTCACCCTGTGGGCAGCTGAGCCTGGAGCAGCCCGAGCC

ATGCTGCTCCGACAGCCCTTCGAGAAACTTCAGATGTCATCAGATGATGGCACGAGTCTCCTTTTCCTGGACTTTGGGGG

TGCTGAAGGAGAGATCCAGCTGGACCTGCACTCGWGTCCCAAAACGATGGTCCTTTATCATCCACTCTTTCCTGTCCGCC

AAGGTCACCCGCTTGGGGCTCTTGGCCTAGAAGCTGTCAGATGCACCAGCCCTGAAGAGGATCGTTCATCTTGTGGCCTG

ACCTGTCCTTCTGCTGACTGCCTGCTCGTCTGGGGGCTGAGGGAAGGGAGAGGAGGGGAACTAGGGCCTCANAGACCAGC

CTGAGGAAGCAGAACTCAGACCTGGGA

Clone 0041-T7 sequence

AGGCTGTGCGAAGCGGGCACGCGCGGACCCTKCACCGGCCGCGGAGCCGCTATGGGCCCGCCAGGCCCCTGGCGTGGCGC

GGCGCAAGGACCGGCTCCGAGCCAGGATTGAAATTCCAAGATGGATGATCAGGACCCTGGGGGCATTAGCCCCCTTCAGC

AAATGGTGGCCTCAGGAGCCGGGGCTGTGGWCACCTCCCTCTTCATGACACCCCTGGATGTGGTGAAGGTCCGCCTTCAG

TCTCAGAGACCCTCGGCAACCAGCGAATTGACAACTCCCTCCAGATTCTGGAGTCTCTCCTACACCAAATCATCCTCCGC

TCTACAGTCCCCAGGGAAGTGCCTCCTATACTGCAATGGAGTCCTGGAGCCCCTGTACCTGTGCCCAAATGGTACCCGCT

GTGCCACCTGGTTTCAGGACCCCACACGGTTCACTGGCACCTTGGATGCCTTTG GAAGATTGTGCGGCATGAGGGCACT

AGGACCCTGTGGAGCGGCCTCCCAGCCACCCTGGTGATGACCGYGCCAGCTACTGCTATCTACTTCACTGCTTACGACCA

ACTCAAGGCCTTCCTGTGTGGTCAGTCCTTGACCTCTGACCTCTACGCACCCATGGYGGCTGNTGCCCCTCGCCCCGAAT

GGGCACCGTGACAGTTGCAGCCCCTTGNAGCTCGTGCGGACCAAGCTGCAGGCTCAGCATGTGTCATACCGTGAGCTGGC

TTNCTCTGTTAÄGCTGCNG

Clone 00 1 BGH sequence

TTTTTTTTTTTTTTTTTTTTTTTTCACAGAAGACCATCATCTTTAGACAAGGAATGAATGGGGCCAAGATACTGCCTGGC

CCAAGCTCCTCGGGCCAAGCTATAACAAACATGGGGATACAAAAAAGGGCGATCATCAGATTTGGTCTTGAATTCGGGAG

GCGGGAGAGGGGCAATCCTGGGAAGGGAAACAGCAGGAATCCAGGTCTGAGGACAGCCTGGG GACTGGACTGGGAGGGA

AGGCACTTGGCTCAGTCTCCTGATCCCACCCGTGCAAGAGCCGGTTGCTCCCCGACTTTCAGCGGCCCANAGGCTGCTCC TGGTTGAGCCTCTGGAAGAAGCTTTTGCCAAACTCGTAAGTGCTGATCATGATGGCACΑGGAGGGGTGCAGCCTTGATGA TCCTGGGGAGGAAAGCCTGCAAAGAGTCCCCTGGTGCCAGATTCAGCCCGG

Clone 0042-T7 sequence

AAAGCTTCACTTCGCCCTCCAGCCGCGG GGCTGCAGCGCAACTTCCAGATAGCGGAGTGGCCTCAGCTGCGAGCCGAGC

GGWGGCGGCAGCGYYCCTCAGGACACCCGCAGATCACCTTTTCCCCGCGACTTCGCCATGGCTGAGTGCTGWGTACCGGT

ATGCCCACGGCCGATGTGTATCCCTCCACCCTATGCTGACCTCGGCAÄAGCTGCCAGAGACATTTTCAACAAAGGATTTG

GCTTWGGGCTGGTGAAGCTGGATGTGAAGACGAAGTCATGCAGCGGTGTGGAATTTTCAACATCTGGCTCATCTAATACA

GACACTGGTAAAGTTAGCGGGACCTTGGAGACCAAGTACAAATGGTG GAGTATGGTCTGACTTTCACAGAGAAGTGGAA

CACCGATAACACTCTGGGGACAGAGATTGCAATTTGAAGACCAGATTTGTCAAGGWT GAAACTTGACTTTTGACACCCA

CCTTTTCACCGAACACAGGAAAGAAAAGTGGTAAAATCAAGTCTGCTTACAAGAGGGAGTGTATAAACCTCGGCTGTGAT

GTTGACTTTGATTTTGCTGGACCTGCCATCCATGGGTCAGCTGTCTTTGGTTACGAGGGCTGGCTTGCTGGGTACCAAAT

GACCTTTGACAGTGCCAAGTCAAAGCTGACAAGGAGT

Clone 0042 BGH sequence

TTTTTTTTTTTTTTTTCGGTTTTCATAAACGTCTATTTCATCATTGGTGGGTAGCACATTTAACAGTTAAATACATTTAA

ATAATGTATAGGAGGCCGCACCACGGCAGCACTGATAACCATCCAACTAGGAACCAGCCAACAGTGACTGTCTAAATATT

TAAAATACAGCTCTTGCTTCATCATCCTTTGATGTGATCACCTTCTGGGGGAAGGAAGGGGAGCCTGCTGGTCGCATGGA

AATATATTAAGGCCAAATCTTCTGATATTCGTTCCCAGAGGTTTCTTTTAACTGGATTAAGCCTCCAATTCCAAGGCAAG

CCCAAGTTTGTGGCCTCCAGCATTAAAGCTCTTCCCGTCTACCAGAGCAGACAGTGTAAGCTTCACACCAGGCCTCAGAG

TCTGAGTATAGCCCACTCCAATTAAACTAGAGTTGTTGACCTTTGCAGAGATAGAAGCAGTAGGATCCAACTGGTATTTA

GCTGCAATGCCAAAACGAGTGCAGCTGGTACCCTGATGTCCAAGCGAGGNTTACTGAAGTGTCAAAATCTTCACATACTT

TCTGATAAATTTGATCCTCCAAATTCTGTCCCATTATTTACATTTGTGTGNAGCTGGAAGTCCCCAGTCCTGTAGCCGAC

NGCA

Clone 0043 BGH sequence

_{TTTTTTTT TTTTTTTTTTTTAAGTT} ^

CGGGCATCTGTGCAΆACAGATTCATTTAACAGGTCGTAGTTTAAAAAAGTCATAGATACTGTGAGTTCTGTATAAACCGG TGGACGGCAAGTTAGTTCCTTTTGATTTATAAGCCTCAATGTCACCGCAGAATAAΆGAATGTAGCCAAAGAΆAGCATTAT CGGTCACTCGTATAGGACAGWGTTGTTTCTATAATTTGAAGCTTTCTGAATGGACGGGTTCAGGCCTGATCCAACTGTAA AAAGATCACTCAGTGAATAGACTATATGGGAACTGTACAAAGTGTCATTAACTTCCATCATTAATAGCTTACTCAGCACT ATACCACTATTGCTAGTTAAAATAACCTGCTTCTGAGGCCCCACGGAGGGAGGCGGCCTGTGCACGCAGCCTCGATGCCC TGGCCACCCTCATCCCCAGGGCGTGCCATACAGTCCAACAGAAAACTTTGGCTTTAGGAAGGAATCACAGACATTGAAAA GAATGGCTTTAATCATTATTAAATGTGCAGTGGGAAGGAGTGTGCTTCAGATAGTCTGGGCAGGGCTGGCGGCAGGCAGG TCACTCCTGCTGCACAGCTGCAGACACTAGTTTGTCATGACAAGACAATGAGGGAAAGCAGNC

Clone 0044 contig

AAAGAAGAAGAGGGGGCTAAGCTGAGTATAGAGGTGCTCCAGACCAGCCTGCAGAAGGAACTGACTCTAAACAAAGGCCA

GGCCTCCGCCATGGAGCTGCTGCGCTGCCCCACGCTTCGGCGCCTCTTCCTCTGCCTCTCTATGCTGTGGTTTGCCACTA

GCTTTGCCTACTACGGGCTGGTCATGGACCTGCAGGGCTTTGGGGTCAGCATGTACCTTATCCAGGTGATTTTCGGCGCT

GTGGACCTGCCTGCCAAGTTTGTGTGCTTCCTAGTCATCAATTCCATGGGCCGCCGGCCTGCACAGTTGGCCTCCCTGCT

GCTGGCAGGCATCTGTATCCTAGTGAATGGCATAATACCGAGGGGCCATACAATCATTCGCACATCCCTGGCTGTACTAG

GGAAAGGCTGTCTGGCTTCCTCTTTCAACTGCATCTTCCTGTACACCGGAGAGCTGTACCCCACAATGATTCGGCAGACG

GGCCTGGGCATGGGCAGCACCATGGCCCGGGTGGGCAGCATAGTGAGCCCACTGATAAGCATGACTGCCGAGTTCTACCC

CTCCATACCTCTCTTCATCTTCGGCGCTGTCCCCGTGGCCGCCAGCGCTGTCACTGCCCTGCTGCCAGAGACCTTGGGCC

AGCCGCTGCCTGATACAGTGCAGGACCTGAAGAGCAGGAGCAGAGGAAAGCAGAAGCAACAGCAGCTGGAACAGCAGAAG

CAGATGATACCACTCCAGGTCTCAACACAAGAGAAGAACGGACTCTGAAAATGGAGAGGGCGTCACTCAGCACTAAAGGG

AGTGGGGTTCTGCAGGTCCCACCATCTATGAGGAGGGGGAGTGAGTGGAGGGACTGGGCCATCTAAATGCGGAGCCTGCC

ATTCAGAGAAATGCCTCCCCAAAGGTCACTTCAATAGACCTACTAGGAACAAAAGCTCTAACTGTGTGCAGCTTCTTAAG

CAGAATGAGCTCTTCCTCAGCCATCTTCCAGCTCAGTGGTCACTCCTCCAGCTCCTGTCCCTTCCACCTCCAGGACATTG

CAAAGAATCCCAGACAATTAAATAAATTTCTTCTACCTTGAAAAAAAAAAAAAAAAAA

Clone 0045 contig

AGCAGGAACTGAAGTCTTTATGGCCTTCCCACTGGAGACCAGCCGCCTGGAAGGCAGATGGGAGCCTCTTTTTCCTGCAA

TACAGTGGATACAGTTTGCTGTGGCTACTCTGAGTGTTATAGGCTCCAGTTCACTCCTCACCTACATCGCGTTCCAGAGC

CCACAGAAGTCTGCAGAGATAAAGCCACTCTTGTATCTGAGCTTCTCTGACCTGCTCCTGGGAATTTGCTGGCTCGTAAA

GGCACTTCTCTATGGAACTGCAGCAGCCCACAAGGACAGCATCTGCTATAACCTACAGACCGTGGGGGAGATTTTCTACC

TTGCCTCACTCCTCTACACCGTCAGCTACATCTGGTGTCTGTACTCGGAGCTGAGGAGGAAGAGCGGCCAGAGTGAGAGG AGCACGGTTGCCCAGGTGACAGATTATGTTTGCCAAGGTGGTCACATATTGTTCCTTTTGTCAAGCCTGATACCTCCGCT GCTGATGACGCCTGTGTTCCTTTTGGGCAATGTCAATGAATGTTTCCACAACTTCAGTGAGAGCCACAGGTGTATCCTAA TGCACTCACCGCCATCAGCCACGCCTGAGCCCCTGCCTTCCACCAATACATCTGTCTGCAGCACACTCTATTTTTATGGT GTCACCATTTTCCTGGCCAGCTTTTTCTTCAGCTTCCTCACCATCATGGTCTTACTCGTCCATGCCCAGATGTTATATAA GAAGTTTTGTRAÄGTCGACGGGCCTTCTGGAGAGTGAGCAGTGGGCAGTGATTCACACTGTGGGCCAGCGAGTGCGTTTC TTNNCAGTGGCCTTTGTGTGCTGCTGGGGTNCAGCTGTCTCCCTGCTGATCATGAAGCTGACAGAGCCTCAGGAGACCTT CCTGCACATGGCCCTCTCTGTGCTCCAGGCTCTCTCAGCAGCATCCCAGGGGCTGCTCAACTGTGGCWTTTATGGCTGGA CNCAGTGCAAGTTCCAGCAGATAAAGCGTGAGCCTCACCGTGACGCAGACACGCAGACCCCWCTGCTGTGCTCTCAGAAG AGAATTTATAGTAGAAGCCCAAACCTACTGGAGTCTCCTCTTGCCCTTGCTTCCAGCTCCTCCACCGTTCTTTGAGACCC TAGAATGGGAATGTCAGGGAGTGAATGTTTCACATGAGTGGACTCTCCTCTGGCCAAACCCGAGACTGGAGAGCTGAAAG GAAATGTGGGCTCAGGGTCAGTAGCAATTCTGGGTGAATTCAGAAGCAGCTCTCTAACTGCTCCTTCAGAGATATACAGC CGCTTTCCAT TAAGAGACTCGTTACTTTGACAAGAAAAaATAAATCTCAGGTTCATAAAAAAAAAAAAAAAAAAA

Clone 0046 contig

AAAAGTGGGCCCCGAGGTCGCGTCTTGCCCAAGTCTCCGCGGTCCCCAGCGCTCGCTCGCGCGGTCCTGCCACGGCCTTC

CTGCTGCCCGCGCCATGGGCCTCAGCTCCAACTCCGCTGTGCRAGTTGAGTGGATCGCGGCCGTCACCTTTGCTGCTGGC

ACAGCCGCTCTCGGTTACCTGGCTTACAAGAAGTTCTACGCTAAAGAGAATCGC CCAAAGCTATGGTGAATCTTCAGAT

CCAGAAAGWCAACCCGAAGGTGGTGCATGCCTTCGACATGGAGGATCTGGGGGATAAGGCCGTGTACTGCCGATGCTGGA

GGTCTAAAAAGTTCCCCTTCTGCGATGGGGCTCACATAAAGCACAACGAAGAGACTGGCGACAACGTAGGACCTCTGATC

ATCAAGAAAAAGGAAACCTAATGGACAGTTGCGAGGCTGCACCCAGCGTGTTGTGATGTCACCTGCTGATTTACGTAGAA

TGGCACCCAACCCACMBGTCTGATTGGCCTCCCCGGTTCTAGATGTGGTTGGTCCCTGCAAATCACAGCTCTCATATCCA

TGGCATCGGCCTTGCTACTGAAACATGTGGTGCACGTTTGTTGAAAGAAGAAGAAAGGCTAAACCAACCTCGTGCTATAT

GGGTTATTTTGGTCTTGTAAGGATCCGTTCCTTTAAAATAATGGTCTTAGAATATAGTTGTATCTTGAGGTTAAAGTATT

AAATTATTCCAAAATCATG ACA G AAAAAAAAAAAAAAAAAA

Clone 0047 contig

AAAGGAGCAACGCGGGTCTTCCCGCTGTTGCTTGTCGCGGCCACGGCCGAGCATACGTCCCCGGCCTGAGGTGGTGGTGG

CGAGCCCACCGCCGTTTGCTGCGACCTCATGGAAGGTGGCGGAGGAAGTAGCAACAAATCCACCAGCGGGTTAGCTGGCT

TCTTCGGAGCCGGAGGAGCGGGTTACTCGAACGCTGATTTGGCCGGCGTCCCGCTGACTGGTATGAACCCCCTGTCTCCT

TATTTAAATGTGGATCCACGCTATCTCGTTCAGGATACTGATGAATTTATTTTGCCAACTGGAGCTAATAAAACCCGAGG

CAGATTTGAACTAGCTTTCTTTACCATTGGAGGATGTTGCATGACAGGGGCCGCATTCGGGGCAATGAACGGTCTTCGTT

TAGGATTGAAGGAAACCCAGAGCATGGCCTGGTCCAAACCAAGAAATGTACAGATTTTGAATATGGTGACTAGGCAAGGA

GCACTTTGGGCTAATACTCTAGGCTCCCTGGCTTTGCTCTATAGTGCTTTTGGTGTTATCATTGAGAAAACACGGGGTGC

AGAAGATGACCTCAACACAGTAGCAGCTGGAACCATGACCGGGATGYTGTATAAATGTACAGGTGGTCTTCGAGGAATAG

CACGCGGTGGCCTGGCAGGACTGACACTCACCAGTCTCTATGCACTGTATAACAÄCTGGGAGCACATGAAAGGTTCCCTG

CTCCAACAGTCACTCTGAAGATTTTTGGCACCTCAGGAATGGGGGACACTTCATAGTCATCCAGATCAATTAATAAGCCA GTCTGGAGTTGCTTTCTGTCTTGTACCTACAGTTACTTTGAACAATTGGAGACTCTGATTTGCTGTGACAAAGATCATGG ATGGTTAGCCTGGACTGCACTGCTTGCCCTCAGTGAGTGGAAGCCAACCCCTTGGTGGCTCACTGAGTATGTGTTCTCTT CTCCTTTGGAGATGATCTTGCACCCCAAACTGGAAAACCACTTACTCTCCAAATTCAGGCCATACTTTTTATTACCATGT TAATTCATTTAAAAAATCTAAAΆCTTAAATGTTGCCCTGTTTTCCAAATAAAGGGTGAAAACAGAAAAAAAAAAAAAAAA AAΆAAAA

Clone 0048 contig

AGGGCCTCCGCACTTCCAAGTCATCTGCCGGCTACTTGGCTACCAGGGCATTGCAGTAGTCATGGAAGAGCTGCTGAAGG TTGTCAAGAGCCTGCTGCAAGGCACAATCCTACAGTACGTGAAAACTCTGATGGAAGTGATGCCCAAGATCTGCCGGCTT CCGCGACATGAGTACGGCTC CCTGGCATCCTGGAG TCTTCCACCACCAGCTGAAGGACATTGWGGAGATGCASNAGC TGAAAACCGTATGCTTCCAGAACCTGCGGSAGGTGGGMAATGCTGYYCTCTTCTGCCTGCTTAT GAGCAAAGCCTGTCT TTAGAAGAAGTCTGTGACCTGTTGCATGCAGCTCCTTTCCAGAATATCTTACCTCGAATCCATGTAAAAGAGGGGGAGAG AGTTGATGCCAÄAATGAAΆAGACTAGAATCCAAGTATGCCCCACTGCACCTTGTCCCACTGATTGAAAGGCTGGGGACCC CACAGCAAATTGCAATTGCAAGAGAGGGGGACTTGCTGACCAAGGAGCGTCTCTGTTGTGGTCTGTCCATGTTTGAAGTC ATCCTGACACGGATCCGGACCTTTCTGGATGATCCCATCTGGCGTGGGCCCCTACCCAGCAATGGTGTCATGCACGTGGA TGAGTGTGTGGAGTTTCACAGÄCTATGGAGTGCCATGCAGTTTGTTTACTGCATTCCTGTAGGGACACACGAGTTTACAG TGGGAGCAGTGTTTTGGAGATGGGCNTCCWCTGGGCTGGCTGCATGATCATTGTACTTCTTGGACAGCAGCGGCGCTTTG CTGTGTTGGATTTCTGCTATCATCNTCTCAAAGTTCAGAANCATGATGGCΆAAGATGAGATCATCAΆAAATGTGCCATTG AAGAAGATGGTGGAGAGGATCCGCAAGTTCCAGATTCTCAACGATGAAATCATCACTATCCTGGACAAGTACTTGAAATC TGGΓGATGGCGAGAGCNCNCCTG TGGAGCATGTACGCTGCTTCCAGCCNCCCNTCCTCCAGTCCCTAGCCNGTAGCTGAG GGCTAAGCTCNCAGCATCCCAGTAACTGATGGCATGTTTGTCTTTATGTAAACTATATTGAAATTTTTAGGGGCTATTTT CCNTTATGTCTGAACCTACTTTTGATCTGAAAGCTTAACTTTATAAAATTTACTTATTTTTCTATACTAAAATTGTATAT GCCTTTGGAATTGGGAAGTCTGAGAATACGGTTACTAGTTGTTGCCNTTACGGTATTAACAAGTATTTTCATGCCGAATA TTACAGCAGNTA Clone 0051-T7 sequence

AAAGCCTGTGTACGCCACCATCGGCTYYNGGTATCGTCAACACGGCCTTCACTGTGGWGTCGCTGTTTGTTGTAGAGCGA

GCTGGACGACGGACCCTGCACCTCATTGGCCTGGCTGGCATGGCAGGCTGWGCTGTGCTCATGACCATCGCCCTGGCCTT

GCTGGAACGGCTGCCTTGGATGTCCTATCTGAGCATCGTGGCCATCTTTGGCT GTGGCCTTCTTTGAAGTAGGCCCTG

GTCCTATTCCATGGYTCATTGTGGCCGAGCTGTTCAGCCAGGGGCCCCGTCCTGCTGCTATTGCTGAGGCTGGCTTCTCC

AACTGGACCTCAAACTTCATTGTGGGCATGTGCTTCCAGTATGTGGAGCAACTGTGCGGCCCCTACGTCCTTCATCATCT

TCACGG GCTCCTCGTGCTCTTCTTCATCTTCACCTACTTCAAAGTCCCTGAGACCAAAGGCCTGAACCTTCGATGAGAT

CGCTTCCGGCTTCCGGCAGGGGGGTGCCAGCCAAAGTGACAAGACACCCGAGGAGCTCTTCCACCCTCTGGGGGCGGACT

CCCAAGTGTGAGGAGCCCCACACCCAGCCCCGGCCTGCTCCCTGCAGCCCAAGGATCTCTCTGGAGCACAGGCAGCTAGA

TGAGACCTCTTNCGAACCGACAGATCTCGGGCAAGCCGGGCCTGGGCGCCTTTCCTCAG

Clone 0052-T7 sequence

AAAGCTTACCCAGCAGSRNGAGTAGATTTGTATCAACAAGAGAAGGCACGGATGGGCTCAGCGGGACTCAGAGTTCTTGC

CTTGGCGTCTGGTCCGGAACTGGGGCAGCTGACCTTCCTTGGCCTGG CGGAATCATTGACCCTCCTCGAACTGGTGTGA

AGGAAGCTGTCACAACACTCATTGCCTCAGGAGTCTCCATYAAAATGATCACTGGAGATTCTCAGGAGACTGCAATTGCC

ATCGCTAGWCGTCTGGGATTGTACTCTAAGACTTCACAGTCCG GTCTGGGGAAGAAGTCGATACAATGGAGGTGCAGCA

CCTTTCACAGATAG GCCAAAGGTTGYAGTATTTTACAGAGCAAGCCCAAGACACAAGATGAAAATTATTAAGTCTCTAC

AGAAGAACGGGGCAGTTGTAGCCATGACAGGAGATGGGGTAAATGATGCAGTTGCTCTCAAGGCTGCAGACATTGGAGTT

GCGATGGGCCAGACTGGCACCGATGTTTGCAAAGAGGCTGCAGACATGATCTTGGTGAATGATGATTTCCAAACCATCAT

GTCTGCAATAGAAGAGGGTAAAGGCATTTATAATAACATTAAAAATTTTGCTAGATTTCAACTGAGCACGAGTATAGCAG

CATTAACTTTAATCTCATTGGCTACGTTAATGAACTTTCCTAACCCTCTCAATGCAATGCAGATTTTGTGGATCAATATT

ATAATGGATGGACCTCCAGCTCAGAGCCTTGGAGTAGAGCCAGNGGATAAAGATGTCATTCGAAAACCGNCTCGAAA

Clone 0052 BGH sequence

TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCCYTTAAATGATTGAGTTTATTTGTGKGTTAGTAAGAAA

GCCCYAYAACCATAAATAGTACAATATTTAAAAGTAAAAAAAATATTTATATCCATCTAAGACAGACAGTGTATTTGTAC

ATTAGAATTCTTTAAGTGCAGAAGGTGGTTCAGGTTTTGCCTTTTGKATTTTTTAATTCAGTACTTGCCCATATGTTGAA

GTTCACTCAATTGCAAAAGCCAATTCAAATCAAGGAATGTGTTATTCAAAGKTGCATAATTTCATTTGGGACTACCAACA

AAGTTCAAATCAAGTCTTTAACATTAATGGGTCATTTTTAGTCAATGGAAAGTTAAAAAGTCCTTAAGTCTTCATTATCT

TCTTGAACTTGCCCTTCTAAATGATTCTNAGACTGCANTNCCTAGTTTGCNAATTAAAAAAAAATGCNNTATGCATCATA

CTTNAAGGAAAGACGATGAANGCTNAACCAGCNTTCTTCCTGGACCTTCTCCCGGCTCCTTTCANCCTTCTTTATAACCC

TCAGNCACA

Clone 0055 contig

AGGATTGGCGGGGTTCTCTTTGACCACATAAATCAATTCTTTCTTTTGGGAGCCTCAATGGTGGCAA.CTGCAGCGGGACT

TTATCTCATCCCGTTCTGCAAGACAGCAGTCTTATTGATCATCACCATGTCTGTCTTTGGAGCTTCCGTTGGTGTTGTGG

ATACAGGTGCAAATGTTCTCATCTTGGATCTCTGGGGGGACAAAGGAGCCCCACAAATGCAGGCCTTGCACTTCAGTTTC

GCεTTGGGTGCCTTTCTGGCTCCCCTGCTGGCTAAGTTGGCCTGGGGTACAGCACCTGCTCAGAACCACACCGAGTCCGA

CCTTGACACTCTGATGCTGAACCGATCCTCCAACGGCACCTCAGACTCCGTGTTTGCGGTACCCGATGACATGAATCTGC

TGTGGGCATATGCTTCCATTGGCACCTTTATTTTAGTAGTTTCTGTCTTTCTGTTTGGTCTGTTTTGTAAGAAACATTCA

AGGCAGAAAAAACCCAGAGCATCTGCTGAGGGAGCTCGAAGGGCTAAATATCACAGGGCCCTGCTATGCCTGCTCTTCCT

CTTCTTCTTCTTCTATGTGGGAGCCGAGATAACATACGGCTCTTACATATTCTCCTTCGCCACCACCCATGTTGGCATGG

AAGAGAGCGAGGCAGCTGGCTTGAATTCCATCTTCTGGGGAACCTTTGCAGCCTGCAGGGGCCTGGCCATCTTCTTTGCG

ACATTCTTACAGCCTGGAACCATGATCGTGCTGAGCAACATTGGCAGCCTGGTCTCÄTGTTTCTTTCTGGTACTTTTTGA

CAAGAGCCCTCTTTGTCTCTGGATCGCGACTTCTGTGTATGGAGCCTCAATGGCAGCCACGTTTCCCAGCGGCATCTCCT

GGATTGAGCAGTACACCACCTTAACTGGGAAATCTGCAGCATTCTTTGTAATTGGCTCTGCCCTGGGAGATATGGCCATT

CCAGCGGTGATCGGAATTCTTCAGGGACACTACCCAGATCTGCCAGTAGTTCTGTACACATGTCTGGGCTCAGCCATATT

CACAGCTATTTTATTTCCTGTGATGTATAAATTAGCTACCTTGCCCCTGAAGAGAGAGGACCAGAAAGCTTTGCCCACTA

GTTCTAGACTGTGAGGAAGAGACTACATGAGAACTTAAAAAAAAAAAAAA

Clone 0056 contig

ATGCAATGGCGGACGTGTCTGAGAGGACGCTGCAGGTGTCCGTGCTAGTGGCTTTCGCCTCTGGAGTGGTCCTGGGCTGG

CAAGCGRATCGGCYGYGGAGGCGTTACCTAGACTGGAGGAAGCGGAGGCTGCAGGWCRRGCTGGCAACGACTCAGAAAAA

GCTGGACCTGGCCTGAGCACGCGCTGCAGCCCGAGTCCGCCGGGTTCTCACTCCCTAAGCCCAACGCAGCCCGGATCGTG

GGAGCCGCGCGACCCAGGAGTCGTCCTTGCACGGCTTGCÄAGAACATGGCTTGCTTCAGAAAGAAÄATAGTTTTGTCTTC

TCTAACAACTTACτTTCAGCTTGTCGAAGATGAAAATAAAAAGCACTGGAGAGÄÄATAATTTCTTGCACTTTATGAATCT

ATTTTTAAAATAAAAAAATTAAACATCTTTGAATCTTTTTCCTCCTCACAAAAGAAAGCAGTATTTTTGCCTACCATTCA

GTTTGCTGCAGTAAGAGÄTTTGGAGCCTGAAAGCAGAGACTTTCTGATGGAATCTCACCTTGGTACÄGCCTGGAGGCAGA

TCTGATCAACGGACCATTATGAGTCATTTTTCTAGACATATTCAGAAAACCTAGGAGCTGTGTCAAATGCCTGAATTAAG C _ATTACAA A _Τ GCAAGATA _TTT GC _A CT _T TGAAGAATGTAGAGAGTAAAAAAACTAAAATTAAAAAAAATAATGCATGTGAT ATAACGGAATATATATGTGAAAGΆGAAAAAAAAAAAAAAAAAAAAAAA

Clone 0057 contig

AG _ATAGTT T _T GAAC _T TCCG _ATT CCCCTGCCTCCCCACCCCAGGGCTGTGATTGCAGGTGTGCGCTTGGGACTGACCCCAG GC _TT TG _T CGGTGCT _A GGGC _A GC _ACT CTGCGACTGAATTAGGTCCCAGCCACTTCTCTGTCTTTTAAAAGAACAAΆACATT GC _TAAAT GTGCCATTGTTGCTT _T GAGTTTTAATTCTTTTTTTTTTCTTTCTTTCATAAAΆCATTACAGTCTTAAGATATT AA _A GACTTT _T ATTCTGGTTCTATTTCTGTCTTTTCACTCAAΆACTGGTTTTACAAATGATGCCTTGTTTACAGAAAGCTC _T C _TA CC _A CAGGGCCTAGTCATGTGTAAAGTCTCAGTTTCTCTCTGGAGTATCTTGGAGCCTAGCACACTGGCTTTAAAGG AC _A CAGCTAAGAAGCTGATATCTTGACAGTGTTTGTAGACCTTTGTTATAAAAATGAATGTCCTGGAAAGGGTTGGGAGG GAGTTCAΆCA _A CAAAGAAACAAGAATGTCATGTTTAAATTTAATAGTTGTCTAAAATGTCATCTCAAGTCAΆGTCACTGG _T C _TGTTT GC _ATT TGATAGGTTTTTATACTAACTAGCATTATAAGATTATTTCATAATTAGAAAATACCTGTGGATATTTG _TATAAAA G _T G _T GA _AA TAAATTTTTTACAAAAGTGCTCATCGCTTGTTAACACAGCATCATGTATGTGAAAGCAAACTCTA _A G _ATTATAAAT GAC _ΆA CC _T G _A G _TT GCCTTTCTTTGTATTTCATCAΆGCCAAAGTAAAGCTTTCAATATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Clone 0060-T7 sequence

_A GC _ATC CC _TC C _A TGGCCTC _T GC _AT TGGCTYCTGCTTTCTGACCTGCTTGAGTTCCAGTCCTGACTTCCTTGGTGATGAAC

AGCAGTATGGAAGTGTAAGCCGAATAAACCCTTTCCTCTCCAACTTGTTTCTTGGTCATGATGTTTGTGCAGGAATAGAA

ACCCTGACTAAGACAAATTGGTACCAGCΆGAGTGGGGTATTCCTGTGACAACCTGACCATGTTTTGGGGAGGACTGTGGA GGACTTTGGAACTTTGGGCTTAAAGATCCATCCGTTGTTAAGAGCTCTGTCAGATAGGGAGGGG

TGTTGAGAACACTGCAGAAGATGGAGGTCTGGTTTGTGAAATTTCAGAGGGAAAATTAAAGACTCTTTTCAGGGCCATTG

CTGTTTTGAATGTGAAGATTCTGTAGTTCTGGWTAGCTGGGGCTGAΆGAATCAGCTGTGATTAACAAGATACCAGAACTA

CCAAAGCAAAAACTTTGCATTAC _T GGGACTATTGATGCTGGTTAGCTGGAGCTAAGAAATTAGCGGTGATTAAGAAGAGA

CCAGC _A TCATTGAGGTGACATCTTCTGGGAAGTGTTTTCTGAAAGCACAAAGATGCTGTGTTCCAGAGATGGCCAAGGTT

GTACTCCTGCTGCAGCAGGACTTGGTAATATGTAAGGGTCACCCAGGTGGTACTGGTTTTGAAGGCATGAANGGGTCACG

CAAAGCAGCTGANGCTCGGCACTGTGAGAGGCCATGGAAGGCCATTGCTGAAAGTACAGCCTCAGTT

Clone 0063 contig

AAACGGCAACACTTCGGTCAGGATATCTTCTACCAGACACCAAGGCGTATGGAGATAGAATAGAAAGAATGCTTCGCCTC AGTTTAΆACATTGACCC _T GA _A GC _A CAGGTGGAGGAAGAACCAGAAGAAGAGCCTGAAGACACCTCAGAAGACGCAGAAGA CTCAGAGCAGGATGAGGGAGAAGAGATGGATGCAGGGACAGAAGAAGAAGAGGAGGAAACAGAAAAGGAATCTACAGAGA AGGATGAATTGTAAATTATACTCTCGCTATGAATCCCGTGTGGAGAGGGAATGTGAAGTTTTGAAGTCATTTCTTTTGAG AGACTTGTTTTGGATGCTTCCCCAAGCCYCCTTCTCCCCTGCMCTGTAAAATGTTGGGATTATGGGTCACAGGAARAAGT GGTTTTTTTAGTTGAATTTTTTTTAACATTCCTCCTGAATGTAAATTTGTACTATTTAACTGACTATTGGTGTAAAATCT TGTCATGTGTATAAAAATAAAAAAAGRTCCCCAATWAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAΆAAAAAAAAAA AAAAAAAAAAAAAAAA

Clone 0065 contig

AGGGGAGATCCTGAGAACAGACCAGTGGCCTCGGAGGCTCTTCATGCAGCTCATTCCGCAGCAGTTGCTGACCACCCTCG TGCCACTGTTCCGGAATTCACGCCTGGTACAGTTCCACTTCΆCTAAGGACATGGAGACTCTGAAGAGCCTTTGCCGGATC ATGGACAATGGCTTCGCGGGCTGCGTGCACTTCTCCTΆCAAGGCATCGTGTGAGGTGCGTGTGCTCATGCTCCTGTACTC CTCGGAGAAGAAGATCTTCATCGGCCTCATCCCGCACGACCAGAGCAACTTTGTCAACGGCATCCGTCGCGTCATCGCCA ACCAGCAGCAGGTCCTGCAGCGAAGCCTGGAGCAGGAGCAGCAGCAGCGAGGGATGGGTGGCTAGAGGATGCCTGGGCTG GGCGGGCCACAGTCCCAGACGAGGCCCCAGTGGAGACTGGTCAGCTGCTTCTGAGCAGGGGCTCCTGGCGACTGTACCTG CCCAGCAACCTGGAGGACAGCGTCCTGAGGTCTCCCAAGGATGGTCCTTGCCCCTGTATGTTTCCCTAATAAAGCCTTTT ACCCCAAAAAAAAAAAAAAAAAAAAAΆAA

Clone 0066 contig

GGCTGCTTCGTGGAGCAGAGAGGTGCATCACCAGGTTCCCGATGAACCCAGAGAACCCTCCACCGTATCCGGGCCCCGGG CCAACAGCCCCATACCCACCTTATCCACAACAGCCAATGGGGCCAATGGGGCCTATGGGAGCCCCACCTTCCTCAGGGGT ACCCCTACCCACCACCTCAGGGGTACCCCTATCAAGGATACCCACAGTACGGCTGGCAGGGTGGACCTCAGGAGCCTCCT AΆGACCACAGTGTÄTGTGGTGGAΆGACCAAΆCGAAGAGACGACCTGSGCCCATCCACCTGCCTCACAGCCTGCTGGACTG CTCTGTGTTGCTGCTGCCTCTGGGACATGCTCACCTGATCACCTGATGAGCCCAGCTCTTCCGCTTGGCCGCTCTGTGCC ACCTCCGATAAGTGTGCCTGGCCCCATCTCTTCTGATTGCTATAAAGTGGCTAGCTCTGCGCAGACACCTCTACTTTCTG TCCTATGGAGTTΤTAGATTAGTGAGGGTTACTGCTATTTAGTTCAGTGACTTGATCTTTTTAATGTTCAAAACTCATTTC TTACTGATCTTTAAAAAATGTGCTAAATAATTTACTTTTTTGGCCAAAGGCTTAGTTATGAAATATATATTTATAATGTT TAAATTATACACTCAAGGTAATGGCA _A TATGTGACACACTACTAGATCTCTCTGCTCATACCCTTTATGTTTCAATAAAT CTGTCCAAAATCTGGAAΆAAAAAAAA _A AΆAAAAAA

Clone 0067 contig

CTGGAAAGGCGCGGAGCCTGCTGCTGCCATGG GGCTGGTGGCTGGGTΆCACTATGTCTAGACTGGGGGCCCTGGGCGGC TCCCGCGCCGGGTTGGGACTGTTACTTGGTACTGCCGCCGGCCTTGGATTCCTGTGCGTCCTTTACAGCCAGCGATGGAΆ ACGGACCCAGCGCCATGGCCGGAGTCACAGTCTGCCCAACTCCCTGGACTATGCGCAGGCTTCAGAGCGTGGACGCCAGG TGACACAGTTTCGGGCTΆTCCCAGGTGAAGCTGGAGATGCTGCCATACTGCCCAGCCTCTCACAGGAAGGGYAGGAGAAG GTGCTGGACCGCCTGGACTTTGYGCTGACCAGTCTTATGGCGCTGCGGCGCGAGGTGGAGGAGCTTCAGAGAAGCCTGCA AGGACTAGCTGGGGAGATTGTCGGKGAGG CCGCTCTCATATAKAAGAGAACCAGAGAGWGGCCCGGCGGCGCASGTTCC CTTTTGCCAGAGAGAGGAGTGACTCCACGGGCTCCAGCTCTGTCTACTTCACCGCCTCCTCAGGGGCCGCACTCACAGAC GCCGAGAGCGAGGGAGGCTATACAACAGCCAACGCGGAGTCTGATTACGAGCGGGACTCCGACAAGGAGAGTGGAGATGC TGAGGACGAAGTGAGCTGCGΆGACCGTGAGGATGGGGAGGAAGGACTCTCTGGACCTGGATGTGGAGGCGGCGTCCAGTC

CGTC

Clone 0068 contig

AGCGCTGGGTCTGAGTGACCAAAGGCAGTAGCGCTCGCGGAGATCACCCGCTGGCCCTCGATCACCATGTCGGCCTTCGA

CACTAACCCCTTCGCGGACCCAGTGGACGTAAACCCCTTCCAGGATCCCTCTGTGACCCAGCTGACCAATGCTCCTCAGA

GTGGCCTGGCTGAGTTCAATCCCTTCTCAGAGACAAATGCAGCGACAACAGTTCCTGCCACACAAGCTCCTGGGCCCTCC

CAGCCAGCAGTTCTCCAGCCCTCAGTGGAACCAGCACAGCCAACGCCCCAGGCTGTTGCAGCTGCGGCCCAGGCAGGCTT

GCTTCGACAGCAGGAAGAACTAGACAGGAAAGCTGCCGAGCTGGAACGCAAGGAGCGAGAGTTGCAGAACACTGCAGCGA

ATTTGCATGTGCGAGACAACAACTGGCCGCCACTCCCCTCATGGTGCCCTGTGAAACCCTGCTTCTATCAGGACTTCTCC

ACGGAGATCCCTGCTGACTACCAGCGGATTTGCAAGATGCTTTACTATCTCTGGATGTTGCATTCAGYGACTCTGTTTCT

AAACCTGCTTGCGTGCCTGGCCTGGTTCACAAGTGACGCAGCCAATGGAACAGCCTTTGGCCTCTCCATCCTTTGGTTTC

TGATCTTCACTCCCTGTGCCTTCCTTTGTTGGTACCGACCCATCTACAAGGCTTTCAGGTCTGACAACTCTTTCAGCTTC

TTCGTGTTCTTTTTTGTATTTTTTTGTCAAATAGGGATCTACTTCATCCAGTTGATTGGCTTGCCTAACTTGGGGACCAG

TGGTTGGCTTGCAGCCCTATCTACGATGAAAAATGGGCCCTTAGCTGTGACCATCATCATGATGGTAGTGGCTGGCTTCT

TTACCCTCTGTGCTGGGCTTTCACTCTTCCTCCTGCAGCGGGTACACGCCTTCTACCGCCGAACAGGAGCCAGCTTCCAG

CAGGCCCAGGAAGAGTTCTCCCAGGGTATCTTCAGCAGCAGAACCTTTCGCGGGGCTGCCTCATCTGCTGCCCGAGGAGC

CTTTCAGGGGAATTAGGCTTCCCGACTCTCCTTCCCTGAGCCCTGGCCTCTCTGTCAACTGTCTTCTGAGCTGCACTTTC

CTCGGGTGCCTTATGCAGTGACTACACCAGCACAGACCTGGCAGGGATTTGTCTTGTCCCTGCTCCTCCCTCAGTAGTCA

GCTTTCTCCTCTACAAGAGGGAAGGAGAGGAAGGGAGAAGGACTTTAAAÄAAAAAAAAAAAAA

Clone 0069 contig

TTCGTCACTGCCAGGCTGAACTGGAGAGGCTTTCAGAGTCCCTGAGGAAGAGCATCTCCTGTGGAGCTTTCTCTGTTCCT GGGGGTCACAGCCTCTACTTAGAAGCCAGGAAGAAGATTGAGCTGGGCTACCAGCAAGTGCCGAGGAAGGGAGTGAAGGC AAAAGAGGTTCTCAAGAGTTTCCTACAGTCACAGGCTATTATGGAGGACTCTATCTTGCAGTCAGACAAAGCTCTTACAG ATGGAGAGAGAGCCATAGCAGCTGAGCGGACAAAGAAGGAAGTGGCTGAGAAGGAACTAGAGCTGCTGAGGCAGAGACAG AAGGAGCAAGAGCAAGTGATGGAGGCTCAGGAGAGAAGCTTCCGAGAAAACATTGCTAAACTTCAGGAGAAGATGGAGAG CGAAAAGGAGATGCTGCTGAGGGAGCAGGAGAAGATGCTGGAGCACAAGCTGAAGGTCCAAGAAGAACTGCTTATTGAAG GATTCAGAGAGAAATCTGATATGTTAAAGAATGAAATAAGTCACCTGAGAGAAGAGATGGAAAGAACAAGAAGGAAACCC TCACTGTTTGGTCAAATCCTTGACACCCGTTGGCAATGCGTTCATTATGATTTTACCAGGAGCTGGTAAACTATTTGGTG TGGGGCTGAAATTCCTCGGCTCACTAAGTAGTTAGTCTAAAGAGTTTCTGGGAAGGAATTTATAAAATCCAGGCTTCTGG TATTTTGATACCAA _A C _T G GC _TTTTT _K G _A TTTTTAAGTACACATTCTAGTTTAATAACCACGGTTACMAAGAAAGTAAGAA CTTYCATTAATAGCTAΆATTGTCAG _AT ACTGCTTCCTCGGAGTCTAATTCTGTGTCΆGTTCATTACCTATTATATTTAAG GTTÄAAGATNCAAACCAATGATTGAAGAATATAAGGGTCTGGCGTCCATAAΆTCCTGTCAAATTTCAGTGATGTTATCTA AATAATAAAG _A GGGAGGCC _A G _T C _A G _AAT ATAGTGAAATTATTTCTAGAAAAGATGTAAGTAAATCAAACAGAAACATGGC CAAATGAAGACMCATTGGCATATGTATCCCAGTTGTACACTACAGATAAATTGTTAAAAAGAGCTCTTGTTAAAACGCMC AAGTGGGTTTTCATAAGAGAATGTATGAGGAAAATGTTTGATTGATAATAAAAGGCATGCAGATTAAAATAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Clone 0070 contig

AGAACGCCTCTGGGGAGCATGGCATCGTGGTTTTCTCTTTGGGATCCATGGTCTCAGAGATTCCGGAGAAGAAAGCCATG GAAATTGCTGAGGCTTTGGGCAGAA _T TCCTCAGACGGTCCTGTGGCGCTACACCGGAACTAGACCATCGAATCTTGCAAA GAACACAATTCTTGTCAAATGGCTACCCCAAAATGATCTGCTTGGTCATCCAAAGACTCGGGCATTCATCACACACTCTG GCTCCCATGGTATTTATGAAGGAATATGCAATGGAGTTCCGATGGTGATGATGCCCCTATTTGGCGATCAGATGGACAAT GCCAAGCGCATGGAAAC _T CGGGGAGC _T GGGGTGACCCTGAATGTCCTTGAAATGACTGCTGATGATTTGGAAAATGCCCT TAAAACTGTCATCAACAACAAGAGCTACAAGGAGAACATCATGCGCCTCTCCAGCCTTCACAAGGÄCCGTCCTATAGAGC CTCTGGACCTGGCTGTGTTCTGGGTGGAATACGTGATGAGGCACAAGGGGGCACCACACCTGCGCCCGGCCGCCCATGAC CTCACCTGGTATCAGTACCACTCCTTGGATGTGATTGGCTTCCTCCTGGCCATTGTGTTGACAGTGGTCTTCATTGTCTT TAAATGTTGTGCCTATGGCTGCCGGAΆATGCTTTGGGGGAAAGGGGCGAGTGAAGAAATCACACAAATCCAAGACCCATT GAGAAGTGGGGGGAAGTGAAGGAGAAGTATTAGTTCATTATCTGATCAGTTGAACYTTGGAAACAΆGTGTTAGATCCATA TTGTTTTTGTTAGGGAAATAATTCACCATACATTATACATTCAGCACATTTAAAAATAATAATAATAACAATCTAATTGC TGGCCACACCCATCAGGGAAGGTTCTAGTATATGTGATGTGCTTTTCCAGTATCTTCAGTCTAGACAACTCTTGCCATCT GTTGGTAATTTACAGAAAGTCTGGCACTCTGCTTTCAGTGACAGCCCCACAGTTTCCCCTGCTCCCGCCAGCTGATGGTT TCTCCCCTGGATTCTCAGACTGCCGTGGCCTTCTCCAGTGTTAGTCATTCTTCATTGTGTTCATGCATTATGGGTAGCA AGACCTTTGGAGCTTTGGGAGAAGAGATGAGGCTGTGACACTGATGGCCCTGTGTTTGAGATAATAATTGTTGCTTGTGC CGGAATTTGATGAAAACCAAAGTATGTTCTAAGGCAAGTACATCTTCTATTGTGTTCCCAAACCAAGAΆCTTATCΆATAA ATTCATATAAATTGTATCACTAAAAAAAAAAAAAAAAA

Clone 0071-T7 sequence

AGAGAAGCAGACATGGCTCAATTTGCACAGGTCATGGCTGAAGTGGGCGACTTTGGTCGCTTTCAGGTGCGGTTGACCAT CCTGATGGGCATTCCCAATTTCCTGGCTGCATTCTTCATATTTGGCCAAGTCTTCATGGTCCTTGATGAGGCTCACCACT GTTCAGTGTCCTGGGTTAAGAACCACACTTTCAACCTAAGTGCCGCTGAGCAGCTGGCTATAAGCATCCCCAACGACACA GCGGGCAGACCCGAGTCCTGCCTCATGTTCCGGCCACCTCCTGACAGTGCCAGCCTGGAAGACATGCTGAGCCACCGCTT CAATGAGACACAGGCCTGTGACTCAGGCTGGGACTATCCTGAGAACCGGCCTCAGTCCCTAAAGAAAGAGTTTGACCTGG TGTGTGATCGAAAGAACCTGAAGAAGACCTCCCAGTCGGTGTTCATGGCTGGTCTCCTTGTTGGGGCCCTGGTCTTTGGG CCTGTCTGTGACTGGATTGGCCGCAGACCCTCCCTCCTGATGCAGGTGCTTCTGTCAGGCATCACAAGCATGGCCACAGC CTTCGTGTCCAGCTTTGAGCTCTACTTGGCCCTACGCTTTGTCTTGGCTACTGCCAATGCTGGATTTTTACTAAGTACCA ATGTCCTGATTTCAGAGTGGGTGGGGCCΆTCTTGGAGAACACAAGCCGTGGTCTTTGGCCCANAGCAΆCGTTGCCCTTGG GCAGATGGTGTTAGCAGGACTGGCCTATGGTGTCCGAAACTTGGAGACTTCTTCAGATACANGGACCCGACCCGCTTACT GNTCTTCTTCTATTTCT

Clone 0073-T7 sequence

AGCGGGCCTGAGCCGAGCAGCTGCGCGACGTCATGGACAACTCCGGGAAGCAGGCTGAGGCTATGGCGCTGCTGGCTGAA

GCGGAGCGCAAGGTGAAGAACTCGCAGTCCTTCTTCTCCGGCCTCTTTGGAGGCTCATCCAAAATAGAGGAAGCATGCGA

GATCTATGCCAGAGCGGCGAACATGTTCAAGATGGCCAAGAACTGGAGCGCTGCTGGGAACGCTTTCTGCCAGGCTGCCC

AACTACACCTACAGCTCCAAÄGCAAGCACGATGCAGCCACCTGCTTTGTGGACGCTGGCAATGCTTTCAAGAAAGCTGAC

CCCCAAGAGGCCATTAACTGTCTGATGAGAGCAATTGAGATCTATACAGACATGGGCAGATTCACAATCGCAGCCAAGCA

CCACATCTCCATCGCTGAGATCTATGAGACAGAACTGGTGGATGTAGAGAAGGCCATCGCCCACTATGAGCAATCCTGCA

GACTACTACAAAAGGGAGAAGAGTCCAACAGCTNAGCCAACAAGYGTCTGCTGAAGGTGGCTGGCTACGCCCGAACAGCT

GGAGCAGTACCAGAAGGCTATCGACATCTATTGAGCAGGGGGGGACCAGTGCC

Clone 0074-T7 sequence

AAAGTTGTTCTTCTCGTGGTTCCCAGTGGCGAGAGGAGGAGGAAGCCCGGAGCGGAGCGGGGCGKCTGGGGGGGGTGGAC

CCGCCGCGGCTGCTGCTGCCACCGCCGCCGCCGCCACCACCGCTCGTGGGGCTCGTGGCGTGÄGGAAGGAGGACGAGTGA

GACCCCGGGGCGAGCGGGCGGCGGCGCCGCTGCTGCTGCTGCTGCTGCGGGAGGGTCGGCGGCGGGACGGCGATGGCGGA

TATCGACAAACTCAACATCGACAGCATCATCCAACGGCTGCTGGAAGTGAGAGGGTCCAAGCCAGGCAAGAATGTCCAGC

TCCAGGAGAACGAGATCCGAGGACTCTGCCTGAAGTCTCGGGAGATCTTCCTCAGTCAGCCTATCCTTTTAGAACTTGAA

GCACCACTCAAGATATGTGGNGACATCCACGGGCAGTACTATGATTTGCTCCGTCTGTTTGAATACGGTGGCTTTCCTCC

AGAGAGCAACTATTTGTTTCTCGGGGACTATGTGGACAGGGGCAAGCAGTCCCTGGAGACAATCTGCCTCTTGCTGGCCT

ACAAAATCAAGTATCCGGAGAACTTCTTTCTTCTCAGAGGGAACCACGAGTGCGCCAGCATCAATAGGATCTACSGATTT

TATGATGAGTGTAAAAGAAGATACAMCATTAAGCTTGTGGAAAACGTTCACAGACTGKTTTAACTGCTTGCCGATAGCAA

GCCATCGTGGACNAAGAAGATAWTCTGCTGTCATGGAGGGTTATCACCAGAWCTTCAATCTATGGAGCAGAATTCSGCSG

AAATATKAGACCAAYTGATGTACCAGAWCAAGGGCCTTCTTTKGGAACCNTTGGGGGCCTGANCCCCATAAAGAGGCNTT

AAGCTTGGGTGGAAAATGCCCGAGGAGNGGCCTTCCAATTGGTGCCAAAT

Clone 0076 contig

AGGATCATCCGTCTCGCCTCCAGTTCTTCACCGGCTGAGCTGGGTACGGACATCTCAGTGACCACGAGCTAACATAGCAC

GTGAAACTGACCTCAGGAAGAACGAAGCCTGGAGACCCGGAGTGCGACAGCGACCTCCGGACTGAAGTGGAAGAAGCGTA

GCGAGGCCTTCGTGTCCTCGCTGTGGGGGGCGGGGCTTGTTGTGCAGGCGGGGCTCACGTGGTACTTTGGTACTTTGGAT

GACTGAAGGGÄAGCTTGGTACCCGCCTGGTCACTACCCGGAAGTCCAGCCGGAGTAGCACTACCCTGGAGTCCACCCGGC

TGTGAAGCGGTGGTGCTCTTAGGGGTGCTTGACGCCCTCATTGTTAGAAACTGATGGGTACTGTTGGCCGATTCCTAGAC

CCGCTTTTGACCTACGTGATAAATGTGTTTCCGGAACTCTGCCAAAAAGTGTTCTGGAGGTTTTTGTTTGTTTCGGAGCC

AAGGCCTCCCTCGTAGCTCTTAATGGCCTGAAACACTCTTTGTAGCCTAGACTGGCATTGAAGTCAAGTTAGCCCTGCCT

TTGCCTCCCTGGGCTCTGGGATTACCCTGCCTTTCTTGATTATTTCTATTTGAACAAATATGAACAAACTATGTGGCCAC

CACAAAAAAAAAAAAAAAAAAAAAAAAA Clone 0077 contig

AGATGGAGCCTGCGTTTCACAGAGGGGATCTCCTTTTCCTCACGAACCGAGTTGAAGATCCTATACGCGTGGGGGAGATC GTTGTTTTCAGGATAGAAGGAAGAGAGATTCCTATAGTGCACCGAGTCCTGAAGATCCATGAAAAGCAAGATGGGCATAT CAAGTTTTTAACCAAAGGAGATAATAATGCTGTTGATGACCGAGGTCTCTATAAACAAGGACAACACTGGCTGGAGAAGA AAGATGTTGTGGGGAGAGCAAGAGGGTTTGTTCCTTACATTGGAATTGTGACGATCCTCATGAATGACTATCCTAAATTT AAGTATGCAGTACTGTTTCTGCTCGGTTTATTTGTGCTGGTCCATCGTGAGTAAGAAGTCGGACTCCCTGTTCCTΆGGAA GCTGCTGTGCTTGTTGTTACTGAATGTTGGAGTAGATCCTGATCTGTGATTGCGGATTTTCGGAGGACACACACGTTGGC ACTTCTTGGTAGCCCTGGTTTGCATTGCTTTGTGTTTCCACACCAGAGGCTGTGTGGGCGGGTGCATGTGCACCGTGGAG TGCACACAAGGGGACTGTCAATCACAGGGTTTCATATGTTGTCATTGTCACTCTTTCACATTTTTGTACATCΆGTGAATT TTTTATATTAAAAGGTTGAGCCAAAGCCCCCAGTGTTTGTATTTTGAAGCCAAGCTTCACTTTAAAΆGTGCCTACAGAGT TCTGTAAATGAAAACACAGCTCTGCATGAGTTCAAACCTGTCGKTCCTYCTTACAGTAGGAATGGCACATATTGAGGCGG KCATAAGTCTTAACTTTTCAAAATTTTAΆATAAAAGACTTTGCACΆTTTAAAAAAAAAAAAAAAAAAAAAAAAAAA

Clone 0079 contig

AAAGTTCTTCTCTCACGTGGGTTGGCTGCTTGTGCGCAAACACCCGGCTGTCAAAGAGAAGGGCGGAAAACTGGACATGT CTGACCTGAAAGCCGAGAAGCTGGTGATGTTCCAGAGGAGGTACTACAAGCCCGGCCTCCTGCTGATGTGCTTCATCCTG CCCACGCTGGTGCCCTGGKACTGCTGGGGCGAGACTTTTGTAAACAGCCTGTTCGTTAGCACCTTCTTGCGATACACTCT GGTGCTCAAACGCCACCTGGCTGGTGAACAGTGCCGCGCATCTCTATGGATATCGCCCCTACGACAAGAACATTCAATCC CGGGAGAAATATCCTGGKTTCCCTGGGTGCCGTGGGCGAGNGGCTTCCACAACTACCACCACACCTTYCCCTTCGACTAC TCTGCCAGTGAGTACCGCTGGCACATCAACTTCACCACGTTCTTCATCGACTGCATGGCTGCCCTGGGCCTGGCTTACGA CCGGAAGAAAGTTTCTAARGCTACTGTCTTAGCCAGGATTAAGAGAACTGGAGACGGGAGTCACAAGAGTAGCTGAGCTT

TGGGCTTYTGAGWTCCTGTTTYAAACGKTTTCTGGCAGAGATTTAATATTCTGTTGATTAACTAACAACTGGATATTGCT ATCGGGGTGTTAATGATGCATTTAACCTATTCCGGKACAGTATTCTTATAAAATGAGAAAGGTTTGT

Clone 0080-T7 sequence

TCATACAAACACCCCAGTACCTCCAGCAGCTCCAGGAGTCAAAAACACCAGCAGTGGACCGAGGTCCTACAGCTGCCTGC

AGCCATGTTGGCCTTCAGTCTGCTCGTGCTGGGTCTGCTCGCGGAAGTGGCACCTGCCAGCTGTCAACAAGGTCTAGGAA

ACCTTCAGCCCTGGATGCAAGGCCTCATTGCTGTCGCYGTGTTCTTGGTCCTYGTTGCAATCGYCTTYGC GTCAACCAC

TTCTGGTGCCAGGAGGAGCCGGAGCCTGSGAGCACAGTSMTGAYCRTYGGAAACAAGGCAGATGGRGTCCTGGTGGGAAY

RGATGGMAGRTACTCYTCRATGGCRKCYRGTTTYAGGTCCAGYGAGCAYRAGAATGCCTAYGAGAATGTKCYSGAGGARG

ARGGCARGGTCCGCAGCACMCCSATGTGRCRAGCYKCTMYSYRK CS GTCCCCAGRCTACGCWTAGAGTTGATCCCCA

CCATCTCACCCAGCCACTGCTCTACAGGAATCTACTGAAACAAGCTGACTYCCTCACCTCTCTAGAATCACAGTCATCTY

AGGCCAGGACTCAGACCAAGCCCAGCACACCAATGTGCTGTGACCCYKGGCTTTCC

Clone 0081 contig

AAAGCGCTCCTCGAGGGTCGGTCCCGCCACGTCTCTTCCCGGTCTCTCTGGCTCTGGAGTGCTTTCCCCCCGCGGAGGGT

GGGAGCGCGGGGACAGACGGGCGAGATGAGCACCATGTTTGCAGACACGCTGCTCATCGTCTTTATCTCCGTGTGCACCG

CGCTGCTCGCCGAGGGCATAACCTGGGTCCTGGTTTACAGGACAGACAAATACAAGAGATTGAAGGCAGAAGTGGAAAAA

CAAAGTAAAAAATTAGAGAAGAAGAAGGAAACTATAACAGAGTCAGCTGGTCGACAACAGAAGAAGAAGATAGAGAGACA

AGAAGAGAAATTAAAGAACAACAATAGAGACCTGTCAATGGTACGGATGAAATCCATGTTCGCGATTGGCTTTTGTTTTA

CTGCCTTAATGGGAATGTTTAATTCCATATTTGATGGTAGAGTGGTGGCAAAGCTCCCCTTCACTCCTCTGTCTTACATC

CAAGGACTATCTCATCGGAACCTGCTGGGAGATGACACCACGGACTGCTCCTTCATCTTCCTGTATATCCTCTGTACCAT

GTCAATTCGACAAAACATCCAGAAGATTCTTGGCCTCGCCCCTTCACGAGCTGCCACCAAGCAGGCTGGTGGATTTCTTG

GCCCACCACCTYCGTCTGGGAAGTTTTCCTGAAGGAAAGCAGAATTCTGAATTTCCTGTCATACTTTTTAGACATTCACA

TCAGACTTACMGAGCACCTGGCCACAWTCTAGGTNKGKGTWATTTCTCAGCTATGGATATGA CMAATGAGAACCCTGMT

TTAACTAA GGGA ARTGCTATGGTCACCGGATGGCTTCTTTNAGTAATAAGTGS CYCNNTCTGGKTACCATTTGGA G

GCTTAATGTAACCCCMAACCATCAATCTTTCTTGTCTYACTTGAGTATTTCAGTGTATGTGCCATATACACAGAAGCAGA

CTGTCCATGAACATTAGGAGAAAAATAGTTTATGTAACAACTTCACTGTAATTAGTTGTTGTGTTTTTTAAAAAGCCAAA

TATGTCACTTTGTGTTCGACAATGAGCCAAATTTTTAGATGTTTCAGTTAGAGAAATGTTTGATTTTTTTGCATATTAAA

AAGAGTATTTTCTGTAATCTGAACATAAGGATAAGCATTGGCCCAAAAAGCTAAAATAAAGTTTTGTCATGTTCTTTTAC

CAAAAAAAAAAAAAAAAAAAAAAAA

Clone 0082 contig

AGGCGACATGGGGAGGCGCGGAGGCGACACCGGAAGTGGCTGTGGTCCGGGTCGGCCCGAGGGGGACTCAGCGCCCGCAG

CAACCACCCGCGCGGCGGCGGTCAGAGCTCATACCCGTGCGGGTGGGCGTGGCGGGCGCCGGGACACAGCTCCGACTGTT

CGCTGTCGGCGCGGRGMGKCGCCGCGTCGCTCGCCTTNACCAGCTGCCATGAGCGAGCGCCTCCGCCCCAGAAAAAGGAG

AAGAAATGGCAGTGATGATGACAACCACCCTCCTCCCCAGACCAAAAGGAGCAGTAGGAACCCCATCTTCCAGGACTCCT

GGGACACAGAGTCTTCAAGCAGCGACAGTGGTGGGAGCAGCAGCAGCAGCAGCATCAACAGCCCAGACAGGGCCAGTGGG CCAGAGAGCAGCCTGAGCCACACCATCCCCGGATCCTGCCCCAGCACCCCCCAGCCGATGCCTGAGCAGTCTGCACTATG CCAAGGCCCTTACTTCCACATCAACCAGACCCTGAAGGAGGCTCACTTTCATAGCCTACAGCACCGAGGCCGGCCGCCGA CATGATGCTCCTCCGGCAGTTTCTTGCCTTCTGTGAAGGGACAGCACTGTGCAGATTGGÄTATTTCAACTTAATGATTTG TT TTAAAAGTTGCACACAGAAAAAAAAAAAAAAAAA

Clone 0083 contig

TGCTGAGGTGGGAGTGGGCCCAGCGCCCGGTGGCCGCAGCTCACGCGCAACCTGCGCCATGGCCGCCTCCGCCGCCTCCT CCGAGCATTTCGAGAAGCTGCACGAGATCTTCCGCGGCCTCCTTGAAGACTTACAÄGGGGTGCCGGAGCGGCTGCTGGGG ACCGCGGGGACAGAAGAGAAGAAGAAGCTGGTCAGAGATTTTGATGAAAAGCAACAGGAAGCAAATGAAACGTTGGCAGA GATGGAGGAAGAACTACGATΆTGCACCCCTGACTTTCCGTAACCCCΆTGATGTCTAAGCTGCGAAACTACCGGAAGGACC TTGCTAΆACTCCACCGTGAGGTGAGAAGTACACCTCTGACAGCCGCACCTGGAGGCCGΆGGAGACCTGAAGTATGGCACG TATGCCTTGGAGAÄCGAGCATTTGAATCGACTACAGTCTCAAAGAGCATTACTCCTACAAGGCACTGAAAGCCTGAACCG GGCTACCCAAAGCATTGAGCGTTCTCATCGGATTGCCACAGAAACTGATCAAATTGGTACAGAAATCATAGAAGAGTTGK GGGAGCAACGAGACCAGTTGGAACGTACTAAGAGCAGACTGGTAAATACAAATGΆAAATTTGAGCAAAAGCCGGAAGATT CTTCGCTCAATGTCCAGAAAAGTGATAACCAACAAGTTGCTGCTCTCCGTCATCATCTTGCTGGAGCTAGCCATCCTGGT CGGTCTGGTGTATTACAAATTCTTTCGACACCATTGAACTTCTGTAGGGAAAGCCTTGTGGACCAGCATCTTACTCTGAA TGAATGGTTTTACATAGGGATATGTGTTGCTGCTGTGGGCTAACAGTTCAAGAATGTATTGTCTTGTCAGACTATGGGAG GGAGGGCAATGGAAAΆCCTCGAAAATGTGAAGGAACAGCAACAAGACCAGTATGATATACTAAGGTAATAAATGTTGTTT ATGACTCCTTCAAACTTAAA ÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁÁ

Clone 0084 contig

CTTTTTTTTTTTTTTTTTTTTCCGTTATAATAGGCATCTTTATTTGTAAAAATCCAGATATAAAACGTAATCTTTCAGTC

TTTCCAGGTTTTCCTTTTTTACAAAAACAAAAAGGCACGTATAAACCTTGCCCGCTGTCGTCCCCGTACACGGTGTTTCT

CAGGCAGCCCTCCCCCCCGCCCCGCCCCCCGTTACAGCTACATGCTTCATTCCAGGACGTCTGCATCCCCACATGCTTTG

GTGCTTTCCTACCAGGGTAGAGTTCCGAGCTCCAAGACTTGAAGTACACAAAGAGGGGGTAGGGGTGGGTGCAGTGTGTG

GCACAATGTTCCACGGCGTGCAGGGCAGTGGGCTAGTAGTAGGTCTCCTTCTCCACCCAGCCGCCAGGGCGCCGCCTGAT

AATGAGCTTCCGCACCTCGTCATACACAAAGATGAGAAGGGAGTAGGGGAAGGCACAGAACCACCATGTÄGGTTTGAGGG

GATACATCCTAARGGCTGCCCCCATCCCGGGGCAGTAGGATAAGAAAGCAGCAAGGGCTGTCTCTTCAAAGAGGCCAAAT

ATCAAGATCTTGKTCTTCATTCCCTGCTGGAAGACAGAATTCCTTCTGGTCTTGCAGATGACCAAGTCGGCCCACTGCAC

TACCACAATACTGACAAAGAACGCTGTATGGCAGGTGAACTCCACGATCTTCCTCTGCTCGTAGGTCCACTGCTGCCCGT

AGCTGTCCTCCACATCGTTGACCCAGCGGTCATCCCAGGTCTCTCGGATGCCCAACAGGTGAAAGGGCAGGAAACCGTTC

TCAGCCAGAATCACAAAGTAAGTGAAGAAGCCTCCCAGGGCCTGGATCATACCGATCTGTCCATAGGCCATGCTGATCAG

ACGCTCGTTCACAAGTTTGTCCGTTTTGGGGTTTCTGGGCTGCCTCTTCATGATGTCGCTCTCAGCTTGCTCGTAGGCCA

GGGAGATGGCAGGAACCATGTCAGTGCCCAAGTCAATGCAGAGGATGGTCACAGTCCCCAGGGGCAGTGGAATGTTTGCA

ATAATAAATATCAAGAAGGGGGTGATTTCCGGAATGTTACTGGTTAGGGTGTAAGCGATGGATTTCTTCAAGTTATCAAA

AATCAGACGACCTTCCTCTACTCCAGTCACAATGGAGGCAAAGTTGTCATCCAGAAGGATCATGTCAGCAGCTTGCTTGG

ACACATCAGAGCCAACAATCCCCA

Clone 0085 contig

AGGTCTGCGGCCCTCGCAGAACTTCCAGCAGCGACATGTTGGGCCAGAGTATCCGGAGGTTCACGACCTCCGTGGTCCGT CGCAGCCACTATGAGGAGGGTCCGGGGAAGAATTTGCCATTTTCAGTGGAAAACAAGTGGCGGTTGCTGGCTATGATGAC CGTGTACTTTGGATCTGGGTTTGCCGCACCTTTCTTTATAGTAAGACACCAGCTACTTAAAAAATAAGGATATTTAATTC ATCCCTTTAACAGAΆTGAAGAAAGTTTAAGAGGTGATCTGAAAATTGGATTAAACTCTTGAACTCTTATACTAGAAAAAA TTGTAAATAAACTAATGACATAAAGATTCAAAAAAAAAAAAAAAAAAAAA

Clone 0086 T7 sequence

AGATGAATTCAAAGAGTGCCCAGGGTCTGGCTGGTCTTCGAAACCTTGGGAACACGTGCTTCATGAACTCAATTCTTCAG

TGCCTGAGCAACACCCGAGAGCTGAGAGATTACTGCCTCCAGAGGCTGTACATGCGGGACCTCGGCCACACCAGCAGCGC

TCACACGGCCCTCATGGAAGAGTTTGCAAAACTAATCCAGACCATATGGACGTCGTCCCCCAATGATGTGGTGAGCCCAT

CTGAGTTCAAGACCCAGATCCAGAGATATGCGCCACGCTTCATGGGCTATAATCAGCAGGATGCTCAGGAATTCCTTCGT

TTCCTTCTGGATGGTCTCCACAATGAGGTGAACCGGGTGGCAGCAAGGCCTAAGGCCAGCCCTGAGACCCTTGATCATCT

CCCTGATGAAGAAAAGGGGCGACAGATGTGGAGGAAGTATCTGGAAAGGGAAGACAGTCGGATTGGGGATCTCTTCGTTG

GGCAGCTGAAGAGCTCCCTCACATGCACCGATTGTGGCTACTGCTCTACAGTCTTCGATCCCTTCTGGGATCTCTCGTTG

CCCATCGCAAAGAGAGGTTACCCTGAGGTGACGTTAATGGATTGTATGAGGCTCTTCACCAAASAGGACATATTGGATGG

GTGATGAGAAGCCAACTTGCTGCCGCTGCCGAGCCAGAAAACGATGCATAAAAAAGNTCTCTGTCCAGAGG

Clone 0087 contig AGGGCGCCTGAGTGAAAAGTGTGGCACCATGGCCTCTGTGCTGTCCTACGAAAGTCTGGTACACGCCGTGGCCGGAGCCG TGGGAAGTGTGACTGCCATGACAGTGTTCTTCCCCTTGGATACTGCTAGACTTCGGCTTCAGGTCGATGAGAAAAGAAAG TCAAAAACGACGCATGCAGTGCTCCTGGAGATAATTAAGGAAGAAGGCCTCCTGGCACCATACCGAGGATGGTTTCCAGY TATTTCCAGTCTCTGCTGCTCCAATTTTGTCTATTTTTACACTTTTAATAGCCTCAAAGCAGTGTGGGTCAAAGGTCAGC GTTCTTCTACAGGAAAAGATCTCGTGGTTGGGTTTGTAGCAGGAGTGGTGAATGTGCTGCTGACGACTCCGCTCTGGGTG GTAAACACCAGACTGAAGCTGCAGGGGGCAAAATTTCGGAATGAAGMCATTATACCARCTAACTACAAAGGCATTATCGA TGCATTCCACCAGATTATTCGAGATGAAGGGATCTTGGCTCTGTGGAATGGCACCTTCCCCTCCTTGCTGWTGG CTTCA ACCCTGCCATCCAATTCATGTTCTATGAAGGCTTAAAACGGCAGCTTCTAAAGAANCGGATGAÄGCTCTCTTCTCTGNAT GTGTTCATCATTGGCGCAATAGCCANAGCGATTGCCNCCNCAGTCACCTATCCCATGCAGACGGTACAGTCAÄTTCTGAG GTTTGGACGTCATAGACTGAACCCAGAAAACAGGACCCTGGGAAGTCTTCGGAATGTTCTCTCTCTTCTTCACCAGCGAG TCAAGCGCTTTGGAATAATGGGACTCTACAAAGGCCTGGAAGCTAAGCTGCTGCAGACAGTGCTCACAGCCGCCCTCATG TTCCTTGTGTACGAGAAACTGACAGCTGCTACCTTCNCCGTAATGGGCCTGAAGAGCACACATAAGCNCTGAGGCCCGCC CNCAGGACAGTGTGGAGACGCAGGTG CTGCACAGGGAGAAGAGGAACAGTTCTCCTTTCCCGTTGGTCCCATCCNCNCCN CAGACATTCCAGTCATTGGCTGTAAAGGCATCAAAGGCTAAGCAGGGAGTATAGCCAGCTAAGCCTGTCACCTTTATGGG GTTTTTTGGTGTTTTTATGGTGTTTGGTTGTATTTGGGGGTAGAGGTTAGACTGATATGAAAATATTGAAACCCTGAAAA AAAAAAAAAAA

Clone 0088 contig

AGTAGAGCCCCTACGGTCATGGCGGCTACCGCCCCCAAGGCCGGGGGTTCAGCTCCTGAGGCAGCGGGTTCTGCCGAAGC

TCCGTTGCAGTACAGCCTTCTTCTTCAGTACCTGG GGGCGACAAGCGTCAGCCCCGGCTTCTGGAGCCTGGCAGCCTGG

GCGGGATCCCGAGTCCTGCCAAGAGCGAGGAGCAGAAGATGATCGAGAGGGCAATGGAAAGTTGCGCCTTCAAGGCTG G

CTGGCCTGTGTAGGAGGGTTTGTCTTGGGAGGCGCGTTTGGTATCTTTACTGCTGGCATTGATACCAACGTAGGCTTTGA

CCCAAAGGACCCTTACCGGACACCAACTGCAAAAGAAGTCCTGAAAGACATGGGACAGAGAGGAATGTCCTATGCCAAAA

ACTTTGCCATTG GGGCGCCATGTTTTCATGTÄCTRAGTGTCTGGTAGAGTCTTACCGGGGAAAGTCGGRCTGGAAGAAC

AGCGTCATCAGTGGCTGCATCACTGGCGGAGCCATCGGCTTCCGAGCTGGAGTAAAGGCCGGGGCCATAGGTTGTGGAGG

GTTTGCTGCTTTCTCTGCTGCAATCGATTATTACCTACGGTGAAGGAANCAGCCGGGAAGGANAGAGGACGCCAGCCNCC

TTCANAGCTGCTCTGTGGANCAGTTTCTACACACTTGGACTGCTTCAGGGGTGAAGCCAAGTGCATCCCTCTACAGTTNA

TGGTGTNAAGGACNCNCCTCGCTGCTCGATCGCTCTCTGCAGCCNTTGGAACAACCATACTTTTCCMCTTGAATTCCAAC

CAGGTTTTTCAGGGRAGGTGGTATGGCAGATTGTCTCCTTGCAGTAAMCAGATCAGGAGSCAGTGTGTGGMCTGTTACGC TTAAGCTTTGAACCAGGCTCAGTTCTGMCCTGGCTCAGCTCCCCAGCTTTTGAATAGGATAAG

SCCGGGAAGCTCTTCTTCCTTCTGTGTGCAAGTATCCATCATGAGTCCCAGGGCAGGSCAAAGTAAACTTATGCCATCGA

GGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Clone 0089-T7 sequence

AAGGCCGGGAGGATGCGGCCGGAGCCCGGAGGCTGCTGCTGCCGCCGCCCGATGCGGGCGAACGGCTGCGTCAAGAACGG

GGAAGTGAGGAACGGGTACTTGAGGAGCAGCACCGCCACCGTCGCGGCTGCCGGCCAGATTCATCATGTAACAGAAAATG

GAGGACTGTACAAAÄGACCGTTTAATGAAGCTTTTGAAGAAACACCCATGCTGGYTGCTGTGCTCACATATGTGGGCTAT

GGCGTACTCACCCTCTTTGGATATCTTCGAGATTTCTTGAGGCATTGGAGAATTGAAAAGTGCCACCATGCAACAGAAAG

AGAAGAACAAAAGGACTTTGYGTCCTTGTATCAGGATTTTGAAAACTTCTATACAAGGAACCTCTACATGAGAATCAGAG

ACAACTGGAATCGACCTATCTGTAGTGTGCCTGGAGCCAAGGTGGATATCATGGAGAGAAAATCTCATGACTATAACTGG

TCATTCAAGTACACAGGGAATATAATTAAAGGTGTAATAAACATGGGTTCCTACAACTATCTTGGATTTGCGAGGAACAC

TGGATCATGTCAGGAAGCAGCTGCTGAAGTCCTCÄAGGAGTATGGAGCANGGGTGTGCAGCACTCGTCAGGAAATTGGAA

ACCTGGACAAGCATGAAG

Clone 0090 contig

AGCGGATCTTCGGGCCGGGAGGACATTCGGCCTCTGTGAGCCGCAACCTTGCCCAGCGAGCGGTTGGGNGTTCGCCATCT

TAGGAGGATGTTCTCGTCCGTAGCGCACCTGGCGCGGGCGAACCCCTTCAACGCGCCACACCTGCAGCTGGTGCACGATG

GCCTGTCGGGTCCCCGCAGTCCCCCAGCTCCGCCCCGGCGTTCCCGCCACCTGGCCGCCGCCGCCGNGGAAGAGTACAGT

TGNGAATTTGGCTCCATGAAGTATTATGCACTGAGNGGCTTTGGNGGGGTCTTAAGTTGAGGGCTGACACACACTGCTGT

TGTTCCCCTGGACTTAGTAAAGYGCCGCATGCAGGTGGACCCTCAGAAGTACAAAGGCATATTTAATGGATTCTCCATTA

CACTGAAAGAAGATGGCGTTCGNGGKTYGGCTAAAGGATGGGCCCCAACTTTGATTGGCTATTCCATGCAAGGGCTCTGC

AAATTCGGCTTTTATGAAGTCTTCAAAGCCTTATATAGCAACATACTTGGTGAGGAAAACACCTACCTGTGGCGCACATC

ACTGTATTTAGCTTCTTCTGCCAGAGCTGAATTCTTCGCTGACATTGCCCTGGACTCCTATGGAAGCTGCTAAAGTTCGA

ATTCAAAACCCAGCCTGGKTATGCCAACACCTTGAGGGAAGCTGTTCCCAAAATGTATAAAGAGGAAGGCTTAAATGCGT

TCTACAAGGGCG TGCTCCTCTGTGGATGAGACAGATCCCSTACACCATGATGAAGTTCGCCTGCTTTGAACGTACTGTT

GAAGCTTTGTACAAATTTGTGGTTCCCAAGCCCCGAAGTGAATGTACAAAGGCAGAGCAGCTGGTTGTGACATTTGTGGC

AGGTTACATAGCTGGAGTCTTCTGTGCGATCGTCTCCCNCCCTGCTGACTCTGTGGTCTCTGTGCTGAATAAAGAGAAAG

GCAGCTCCGCGTCTCAGGTCCTGCAGAGGCTGGGCTTCAGAGGTGTGTGGAAGGGGCTCTTCGCCCGAATCATCATGATT

GGCACTCTGACTGCNCTACAGTGGTTCATCTACGACTCTGTGAAGGTCTACTTCAGGCTCCCTCGCCCTCCTCCCCCTGA

GATGCCAGAGTCTCTGAAGAAGAAGCTTGGGTTAACTGAGTAGATACATCAAAGTAACATGGACTGAATCTGCTTGTTGA TCAGTGTTGAAAGTGCAAAGGAACTTTTATATATTTGACAGTGTAGGAAATGTCTGTTCCTGATATAATTACTGTAGTAC CCTTGCTTAAAGCAAGAGTTTCAAACTTAATGCTAAATAAACCCATCTATTCCTGAAAAAAAAAAAAAAA

Clone 0091 contig

AGGCACTGCTGGCGACATGGCCGACACGGACCCGGGCTATCCCCGCTCGTCCATCGAGGATGACTTCAACTACGGCAGCT GCGTGGCGTCGGCCAGCGTGCACATCCGCATGGCCTTTCTCAGAAAAGTCTACAGTATCCTCTCTCTGCAAGTCCTCCTG ACTACAGTGACCTCTGCCCTGTTCCTGTATTTCCAAGCTCTGCGGACATTTGTCCATGAAAGCCCTGCCTTAATTG GG GTTWGCTCTGGGATCTCTGGGCTTGATCTTTGCACTGACTCTGCACAGACACACGCATCCTCTGAACCTCTATCTACTCT TTGCATTTACACTGTCAGAATCCCTGGCCG GGCAGCTGTTG TACCTTCTATGATGTATATCTGGTTCTGCAAGCGTTT ATAATGACTACTGCAGTCTTTCTTGGCTTGACTGCCTATACTCTACAATCAAAGAGAGATTTCACCAAATTCGGAGCAGG GTTGWTWGCTGGTWTG GGATTTTGWGCTTGGCAGGATTCTTGAAGCTG TTTTTTACAG GAGACGATGGAGCTGGTCT TGGCCTCTCTAGGCGCCCTCCTCTTCTGTGGGTTCATCATCTATGATACACACTCGCTGATGCACAGACTCTCTCCCGAA GAGTACGTGAWCGCTGCCATCAGTCTCTACATGGATATCATCAACCTCTTCCTGCACCTGKTGAAGTTTCTGGAAGCAGT TAAATAAAΆΆGTAACCGAGCAGTNGTTCAGAGACAGKTCTATTATGAΆAGGACGCTTTGGAATTAAACTTTAAATGGTTA ATAATTAAAAGCCAWATGTGAWCTTTAAAAAAAAAAAAAΆAAAAAA

Clone 0094 contig

AGGGCCGCAGCAGGCGCGAGCGCCCAGGACCTGCCAGCTGAGCCTTCCGCCGYCGCCATGGGACAGAACGACCTGATGGG CACGGCCGAGGACTTCGCCGACCAGTTCCTTCGAGTCACCAAGCAGTACCTGCCTCATGTGGCGCGCCTCTGCCTGATCA GCACCTTCCTGGAGGATGGCATCCGCATGTGGTTCCAGWGGAGTGAGCAGYGTGACTATATCGACACCACCTGGAGCTGT GGCTACCTGTTGGCCTCATCCTTCGTGTTCCTCAACCTGCTGGGACAATTGACTGGCTGTGTTTTGGTGCTGAGCAGGAA CTTCGTGCAGTATGCCTGCTTTGGGCTGTTTGGAATCATCGCACTGCAGACAATTGCCTATAGCATTTTGTGGGACTTGA AGTTTCTCATGAGGAACCTGGCTTTAGGAGGAGGTTTGCTGCTGCTCTTGGCAGAATCCCGCTCAGAAGGGAAGAGCATG TTTGCTGGTGTCCCAACCATGCGTGAGAGCTCCCCCAAGCAGTACATGCAGCTTGGAGGΆAGGGTCCTGCTGGTCCTGAT GTTCATGACCCTCCTTCACTTTGATGCCAGCTTCTTTTCTATCATCCΆGAACATTGTGGGCACAGCTCTGATGATCTTAG TAGCCATCGGTTTTAAAACCAAGCTGGCAGCTTTGACΤCTCGTCGTCTGGCTGTTTGCCATCAACGTGTATTTCAACGCC TTCTGGACAATCCCGGTATATAAACCCATGCATGACTΤCCTGAAATACGACTTCTTCCAGACCATGTCAGTGATTGGAGG CCTGCTCCTGRTGGTGGCTCTTGG CCAGGGGGTGTGTCCATGGATGAGAΆGAARΆΆAGAGTGGTAACACAC GATCCCT CCCCTCCCTGGCTGAGGCRCAGGGCCCTGGCCTGGTTCAGGGCÄRAGTCAACAAACTGCCGGCGTTTGTGTGTCCTTCTC CCTTCCCCTCCCTTGGTAAA GGCACAGATGTTTTGAGAACTTTATTTGCAGACACCTGAAAATTGGAGATAAAATCTTTG GAAAAAAAAΆAAAAAΆAA

Clone 0095 contig

AGCTTCTTCAGTCGGGACGGGGTCAGCGAGCGGCTGCTTCGTGGAGCAGAGAGGTGCATCACCAGGTTCCCGATGAACCC AGAGAACCCTCCACCGTAΓCCGGGCCCCGGGCCAACAGCCCCATACCCACCTTATCCACAACAGCCAAWGGGGCCAATGG GGCCTATGGGAGCCCCACCTCCTCAGGGGTACCCCTACCCACCACCTCAGGGGTACCCCTATCAAGGATACCCACAGTAC GGCTGGCAGGGTGGACCTCAGGAGCCTCCTAAGACCACAGTGTATGTGGTGGAAGACCAAAGAAGAGACGACCTGGGCCC ATCCACCTGCCTCACAGCCTGCTGGACTGCTCTGTGTTGCTGCTGCCTCTGGGACATGCTCACCTGATCACCTGATGAGC CCAGCTCTTCCGCTTGGCCGCTCTGTGCCACCTCCGATAΆGTGTGCCTGGCCCCATCTCTTCTGATTGCTATAAAGTGGC TAGCTCTGCGCAGACACCTCTACTTTCTGTCCTATGGAGTTTTAGATTAGTGAGGGTTACTGCTATTTAGTTCAGTGACT TGATCTTTTTAATGTTCAAAACTCATTTCTTACTGATCTTTAAAAAATGTGCTAAATAATTTACTTTTTTGGCCAAAGGC TTAGTTATGAAATATATATTTATAATGTTTAAATTATACACTCAAGGTAATGGCAATATGTGACACACTACTAGATCTCT CTGCTCATACCCTTTATGTTTCAATAAATCTGTCCAAAATCAAAAAAAAAAAAAAAAAAAA

Clone 0097-T7 sequence

GTTTCCAACAAGGACGACATGAAGACCTCACTCAAGAAAGTTGTGAAGGAGACATCGTATGAGATGATGATGCAGTGTGT

ATCGCGAATGCTGGCCCATCCCTTACACGTGATCTCGATGCGATGCATGGTGCAGTTTGTGGGACGGGAGGCCAAGTACA

G GGTGTGCTGAGTTCTATTGGGAAGATCTTCAAGGAAGAGGGGCTGCTGGGATTCTTCGTTGGCτTAATCCCTCACCTC

CTGGGCGATG GGTTTTCTTGTGGGGCTGTAACCTGCTGGCCCACTTCATCAATGCCTACTTGGTGGACGACAGCGTGAG

TGACACCCCAGGGGGGCTGGGAAACGACCAGAATCCAGGTTCCCAGTTTAGCCAGGCCCTGGCCATCCGGAGCTACACCA

AGTTTGYGATGGGGATTGCAGYGAGCATGCTGACCTACCCCCTTYCTGCTCGCTGGAGATCTCATGGCAGTGAACAACTG

TGGGCTGCGGGCTGGACTCCCTCCGTATTCCCCTGTGTTCAAGTCCTGGATCCACTGCTGGAAGTACCTGAGTGTGCAGG

GCCAGCTCTTCCGCGGCTCCAGCCTGCTTTTCCGCCGGGTGTCATCGGGGTCATGCTTTGCCCTGGAGTAACCTAAGCTG

CCCGACCAAACATTTATGGGGTCTTAGCCTACCCCTGGTGAGGACCCATCATCTCAGATGCCCCÄAGGGTGACTCCAGCC

CAGCCCTGGCTTCATGTCCATATTTGCCATGTGTCTGTCCAGATGT

Clone 0097 BGH sequence CTTTTTTTTTTTTTTTTTTTCACCACCAATACATTTATTCGAGGAGATGGGTCTATCTTACCACGAGGGGAGGACTAGAT GTCGGYCTATGTAACCTGTGCGTATTCGCACCCAGCACAGTGACTGAACCCTCACACCTGGCGTCACCAGCACAGACAAG CAGATGAGGGGATGGTCTGAGGAGAACATGATTTCCTATTCAGGAGAAGGCACCACCCTTGTATAAGAAAATTAGTGTTG

GGAACATAGCGCCAGCCTCCCATGGCCCAGGTGTGATGGCGCCCAATTTACAAAGCAGGAAGTGGGGGGCGGSGGTGCTT

CTGGCTGACTGGCAGGATGAGCTGGGCTAGAGGTGCAGGGAÄGCCTTGCCACΓGAGTGACGTTTGCCTCTGCAGCCTGCC TCTGCCΓGAGTACAAGATGGACTCCAGTACCTCTAGGCAGGAAGGGGATGCCACCCCACCACT

Clone 0098-T7 sequence

AGGGCGGAGGAAGCGGACTGTTCCGGAGCTCTGCCTAGCCGGGCCCAACCTTTGCTCCAGAGATCATGGCTGCCGAGGAT GTGGTGGCGACTGGCGCCGACCCTGAGCGAGCTAGAGGGCGGCGGGCTGCTGCACGAGATTTTCACGTCCTCCTCTCAAC CCTGCTCCTCCTGGGCCTCTGCATCTTCCTGCTCTACAAGATCGTTCCGCGGGGACCAGCCCGGTGCYAGTGGCGACAAC GACGAYGACGAACCACCCCCGCTGCCCCGCCTCAAGCGGCGCGACTTCACCCCTGCCGAGCTGAGGCGTTTCGATGGCGT CCAGGACCCGCGCATTCTCATGGCCATCAACGGYAAGGTGΓTCGACGTGACCΆAAGGCCGCAAGTTCTACGGGCCTGAGG GGCCATATGGGGTCTTTGCCGGAAGAGATGCATCCAGGGGCCTTGCCACATTTTGCCTGGACAAAGAAGCACTGAAGGAT GAGTATGACGACCTTTCTGACCTCACCCCTGCACAGCAGGAGACCCTGAGTGACTGGGACTCTCAGTTCACTTTCAAGTA TCATCACGTGGGAAAACTGCTGAAGGAAGGGGAGGAGCCTACTGTGTACTCAGATGATGAAGAACCAAAAGAYGAGACAG CTCGGAAGAATGAATGAAGCATTCGGTGGAGCATATCTATTTTTGΓATTTTGCAAAATCATTTGTAACATTCCAGTCTGT CTTTACAACATGGTGATTTCAATATTTAGAAAAGNTTTGAACTTGCTGTAAATTTTTTAAGTAACATCACTAGTGACACC GATAAAATCAACTCG

Clone 0099-Contιg

AGCTCGAAÄGCGACATGGCGGTTCTCTTAAAGCTGGGCGTTCTCTGCAGTGGCCAAGGAGCTCGAGCTCTCCTACTCCGA

AGCCGGGTGGTCAGACCCGCTTATGTGTCAGCATTTCTCCAGGACCAGCCTACCCAAGGACGGTGTGGTACCCAGCACAT

TCACCTGTCACCAAGCCACCACTCTGGTTCCAAGGCTGCATCTCTCCACTGGACCAGTGAGAGGGTTGTCAGTGTTCTGC

TCTTGGGGCTGATCCCTGCTGGGTACTTGAATCCCTGCTCTGTGGTGGACTACTCTCTGGCTGCAGCCCTCACCCTGCAC

AGTCACTGGGGCCTTGGACAAGTGGTTACCGACTACGTTCATGGGGACACCCTGCCGAAGGCTGCCAGGGCAGGCCTCTT GGCACTCTCAGCTTTGACCTTTGCTGGGCTTTGCTACTTCAATTACCACGATGTCGGCÄTCTGCAGAGCGGTTGCCATGC TGTGGAAGCTCTGACCTGGGTGCAGCACTTTGATTGTGTGCCTCCTTGCCTCTGCTΓTACCAATGCCGTTCACCTCGCAG TGAGGGGGGATGAAGGATAAGCCCAΓTGGTGGGCAGAΆTGTCTTCTAATTACATGGTTATTTTCAGAATTTAΓTTGTTGA GGAAGAGGTTΓGAGGAGTTAGGTTCGACCATTCGTGAGTCTGTGTTCCATACTCCACTGAGTGTGGGCACTAGCTCACAG CCTCGCGGTGAGACTGAACATTTCATGAGCTCATGKTGCCTΓTGACCACCATTTTCTTAAGGAGAGCCAGCTGATTGCTG TCAGGATAAGAGCATCTCTTCAGCCAGGAGGGAGGCCTGTΓCCCTCCTGAGTTAGACTTTGCATGAAGCTCGAAAGTATT CCCTTTGGAACCTCCCATTCTTGTTCΆGGTGACACCAGCTCTGTTGATGGCTCTGCΓTCTAGGGAACATTTAATCAGGAG ATGCTCΓCAATGACTAATTTGTCTAAGTCTTAGGAAGGAGGTTGAGGAAAGCTGGATTTAGACAAGTTCAATTTAGGGAG TTCTCCTTGTTTGTGGATTAAAÄTATGACAGATTGCΆAACAGACTACTCTTCAAATGTATCTCAATTGTGCAGAAGTGAG CTGTCCAAAAGTATAAGACTAAGTGATAAACTGTCTTCCCACCGTGGGAGTTGTTAATGAGAAAGAAAGTGTACTCTGAA AAAACAAAATTTTTAAATAAAATATΤATATAATGAAAAAAAAAAAAAAAAAA

Clone 0100 contig

AGCTAGGATCTTTAGTCTTCAACTCCTACTGCTCCTTCTAACCCAGCAGCCCCGGATAATGCAGCCCAGGAGGAGCTCAT GATCACCCTGATCACAGGATTGGCGΓCCCTCACGTCGAGAACCTCCATGGGCATCAΓCGKTGKTGGGGGCCGGTRATTTG GAAAACAGGTGGGCTGGAAACTAATCTCTGTCACCTΓAAGTATGTACGGAGCTCTGTΆCCTTTATGAGAGGCTGACGTGG ACGACCCGTGCGAAAGAGAGAGCGTTTAAGCAGCAGTTTGTAAACTATGCAACTGAGAAGCTGCAGATGATTGTGAGCTT CACCAGTGCAAÄCTGCAGCCACCAAGTACAGCAAGAAATGGCCACTACTTTTGCTCGACTGTGCCAACAAGTCTGATGTT ACTCAGAAACATCTGGAAGAGGAAAΓTGCAAGATTATCCAAAGAGATAGACCAACTGGAGAAAATACAGAACAACTCAAA GCTCTTAAGAAATAAAGCTATTCAACTCTGAAAGTGAGCTGGAGAATTTTTSGAAGCAGTTTCTACACCCGAGCAGTGGA GAATCCTAACGGCAGAGGCACTGTAGGAGGAAGCGGACTTGGAAGATGGGAAATGTTACTTTATGAAATGACCTCAGTAC AAATTACTAACTCTTAGTATCGATGCCTTGCGGAGATTGTGGTAATGACCTGTCTCAGGGGTTGCACCTTTGGAAGTGTT GTGATTCGCCTTGTCTTAGCATTAGTTTGGAGTAAAGACTGAATTGTTAAGGTTAAATGATGAATTCCTTTAGAAACAGT GGAACCGGCTGTKCGGCCCCTGAGGGTGGGTCCTGCAGCTCCTCACCAGGCTGGCTGTCTGCGGCTCTCAGAAGCTGCTT CCTGGCATCCAGGAGTTAGAGACCTTTTCATCCTTTCTCAGTGCTAGTTCTTGATGCTTCTTTAATGGGAATAGTGAACT TGTTTATAASCCGATTTGCTCA AACGAGGGGTGTGGGCTGCTCCTGGGGGGGTCCTGCAATCACTCTGTCCTCACAGCAA GGATGTAACCMCTACTAAACAGTTTTTACTTTCTTTTATTCCCATTAAAGCTGATGTGAAATAGTAAAAAAAAAAAAAAA AAAAAAAA

Clone 0101-T7 sequence

AGTCTGCGCCCCTGTACCCTAGGTCΓAGTAGTGAACCCGCACCCGRKCAAAGAAGTCTA _GG CCGGRGTCCGCAGMATCAG CCATCACTTCGCACAAAGCAGCGCAGCTCCGGGACCGCCGAGGACCACAGCGGCGCGGCAGCGGYGCGGMGACACTCAGT GCACCGTATGCCCCTGCGMCCCTGCCGAGGCAACCGGAGCGCCCCGAGAGACCGCGCCGCCGCGGGGWCCAGGTGCAGYT AGCGAGCCTAGCCCGCAGCGCGCAGTCGCGGGAGAGCGGGGAGCGGCAAGCAACAGGGAGCGGGACGGCGGCGAGGCGCT CGCGGGCCCCTCCTGCTGCCCGCGCCCGGCGAGCTCATGGCGGCCATCCGCAAGAAGCTGGNGGNGGNGGGCGACGGCGC GTGCGGCAAGACGTGCCTGCTGATCGTGTTCAGTAAAGACGAATTCCCCGAGGTGTACGTGCCCACCGTGTTCGAGAACT ATGTGGCGGACATCGAGGTGGΆCGGCAAGCAGGTGGAGCTGGCGCTGNGGGACACGGCAGGCCAGGAGGACTACGATCGT TTACGGCCGCTCTCCTATCCGGACACCGACGTCATCCTTATGTGCTTCTCGGTGGACAGCCCGGACTCTCTCGAGAACAT CCCCGAGAAGTGGGTGCCCGAGGTAAAGCACTTCTGCCCCAATGTGCCCATCATCCTGGTGGCCAACAAAAAAGACCTGN TCAGCGACNAGCATGTCCGACGGAGCTGGCCCGCATGAAGCAGGAGCCAGTNCNGCACGGATTGACGG

Clone 0102 contig

AGGTTTCCTCGCCGCCGGCCAAGATGAACCGATTCTTCGGAAAAGCGAAACCCAAGGCTCCGCCACCTAGCTTGACGGAC TGCATTGGGACGGTGGATAGCAGGGCAGAATCCATTGACAAAAAGATTTCCCGGCTGGATGCTGAACTAGTGAAATATAA GGATCAAATCAAGAΆGATGAGAGAGGGTCCTGCTAAGAACATGGTCAAACAGAAAGCCCTGAGΆGTTTTAAAGCAAAAGC GGATGTATGAGCAACAGCGAGACAACCTGGCCCAACAGTCCTTTAACATGGAGCAAGCTAATTACACCATCCAGTCACTA AAGGACACCAAGACCACGGTTGATGCCATGAAGTTGGGAGTAAAGGAAATGAAGAAGGCATATAAGGAAGTAAAAATTGA CCAGATTGAGGACTTACAAGACCAGCTGGAGGATATGATGGAAGATGCAAATGAGATCCAGGAAGCCCTGGGCCGCAGCT ACGGCACCCCAGAGTTAGATGAGGACGACCTGGAAGCAGAGTTAGATGCGCTGGGCGATGAGCTTCTGGCTGATGAAGAT AGCTCCTACTTGGATGAGGCAGCTTCCGCTCCTGCAATTCCGGAAGGTGTTCCCACTGACACAAAAAACAAGGATGGCCG TGCTGNNGGATGAATTTGNACTGCCGCAGATTCCCGCTTCGTAGACTTACAACATTCAGCACGTGATGTGAAACAACAAG AGAAGTATTCTGGGACTAGGAAATAGTTCCCCGATCTGCCAACCAGATTTAGGTTTCTTTCCTTTCTTTGAWGAAAAAGC TNTNTACACTGCTCCNGNATTTTTATTTTTTCCGNTAAGGAATTCATTGCTTTΤGGGAAACTGTCTTTACTAAAACTTGA TTCNTTTTTTTTTTTTTCTCTTAGGAAAGACTAATTGAAAAGTACCCTTGACTTTGTATGACTTGTTTTCATTCATTAAC AATAATCTGAAATTAAACCAAGGAG ATGAGACTCTGAATTCTATGGTAGNGTAAGTACAGTCTCAGTGNGCTGATACATT GATAAGATAAAAGTGATTGATGAGATTGGGACTGCTGATAGTATGCTTCAGAACCCTTGTCTGTTGTGGTATTGTANATG GGTTTAAGTCATGGCCTCTTTTGATANATTTTGTTGTGTCATGTGAGCAAGTCATTNCACGATNTACTGTTGGAATGAAC TGTCTCTTCGGTATCATGAGTTACTATTTTGATTCCATGGTTCCCTCCAGTATACTAGCCTGRCTTGTAATGAATAATGA ATATTTCTTGATATTTÄATGKATAGGRCMTTTATTTATACTCAAT AATATTTTTCMAAAGGAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAA

Clone 0107 contig

AGGGCGGCGGCCTTGGGCCGCGGGGAATGGGAGTCCCAGGGCCCTGACTGAGCACCGCCCCCGCCTCCCGGCCGCGCCAT

GGCTCAGGAGAAGATGGAGCTGGACCTGGAGTTGCCCGCGGGCGCGAGCCCGGCGGAGGGCGGCGGCCCTGGCGGCGGGG

GCCTGCGGAGGTCTAACAGCGCCCCCCTGATCCACGGCCTCAGCGACTCGTCGCCGGTGTTCCAGGCCGAGGCGCCCAGC

GCCAGACGGAACAGCACGACGTTCCCGAGCCGCCACGGCCTGCTGCTCCCGGCCTCGCCGGTCCGAATGCACAGCAGCCG

CCTGCACCAGATCAAACAGGAGGAGGGCATGGACCTGATCAACCGAGAGACCGTTCACGAGCGCGAGGTGCAGACCGCAA

TGCAGATAAGCCACTCCTGGGAGGAAAGTTTCAGCCTGAGTGACAACGACGTGGAGAAATCCGCCTCCCCGAAGCGCATC

GATTTCATTCCGGTGTCTCCGGCACCGTCCCCGACCCGGGGAATTGGGAAGCAGTGTTTCTCACCGTCCTTGCAAAGTTT

TGTGAGTAGCAACGGACTGCCTCCAAGTCCCATTCCCAGCCCAACCACCCGATTTACCACCCGGAGAAGCCAGAGTCCCA

TCAACTGCATTAGACCAAGTGTTCTTGGACCATTGAAAAGAAAATGTGAAATGGAAACTGATTATCAGCCAAAGAGATTT

TTCCAGGGCATCACCAACATGCTGTCTTCTGACGTTGCACAGCTGTCAGACCCCGGTGTGTGCGTATCTTCCGATACCCT

GGATGGAAACAGCAGCAGTGCCGGATCTTCCTGTAACTCACCAGCGAAAGTCAGCACTACCACCGACTCTCCTGTGTCGC

CCGCCCAAGCAGCCTCCCCCTTTATTCCAGTAGATGAACTTTCCTCAAAGTGACTCACTCATCTTGAATCCCCCTCCCCC

AATGGAGAGACAAAAAAACAAAAACAAACAAAAAAAAAAAAAAA

Clone 0111 contig

AGGCAATGGCGGACGTGTCTGAGAGGACGCTGCAGGTGTCCGTGCTAGTGGCTTTCGCCTCTGGAGTGGTCCTGGGCTGG

CAAGCGAATCGGCTGCGGAGGCGTTACCTAGACTGGAGGAAGCGGAGGCTGCAGGACAAGCTGGCAACGACTCAGAAAAA

GGAGCCGCGCGACCCAGGAGTCGTCCTTGCACGGCTTGCAAGAACATGGCTTGCTTCAGAAAGAAAATAGTTTTGTCTTC

TCTAACAACTTACTTTCAGCTTGTCGAAGATGAAAATAAAAAGCACTGGAGAGAAATAATTTCTTGCACTTTATGAATCT

ATTTTTAAAATAAAAAAATTAAMCATCAAAAAAAAAAAAAAAAAAAAAAAAAAA

Clone 0112 contig

AGGGACTTTGTTCAGATCTTTTTCTGATCGTGCATCAGCTGCTGGTTCATTATAGCCTTCTCTTAAGCACTGAATAAGAC

AAAAAATGAATCTTGGAAGAACAAATTCAGACATCATCAGTAAGTCTTTGGGGACACAGGGAATATTTGAACTTGATTTA

ATCTGATGTCTTCTACAAAACCCGCTCTCCCGCATTACGTTGCTGTCCCCGCAGTCGCAGGCGCCCCCGGCCTGGCTGCG

GAACATGTTGAAGTCGTGTCCAGTGTGGTCGCCCTGGTGGAAGCACTCCGCGCACAGCGACATGCAGGGGGAGATGCCGC

ACGTCCGGCAGCGGTAGGCCACGAAGTTGGCCGTCCAGACCAGGCCGCAGAGCGCCGCGGGGTCGTAGGCCCGCACGGCC

GCGCAGAACTCCTCGTAGCCGCCGCCGCCGGCCAGAAGGCACTTACACCACTCCAGGGCATCCTCCTCCGCCTCGCCCGG

GCCGCCGCCGCCGCCGCCCGCCGCCTCCTCGCCGCCCGCAGCCCCGGCCAGC Clone 0113-T7 sequence

AGGCGACGCGCGCATGGAGGCCGGCTGAGGAGCGCCGCCGCCTCTCTCGGTAAGGACTGTGTCTGTGTCCCCAGGCATCC

TACATCAATCAGGAAGCTGCTGTCCAGCCATGGAGGGAGAGGAGAAGCCAGCTCAAGAGGCTGACGTGGAACCTGTGGTA

ACAGCAGGCACCTCAGAAGCAGTGCCAAGGGTGCTTGCTGGAGACCCTCAGAACATCTCTGATGTGGATGCCTTCAACTT

GCTCCTGGAGATGAAACTGAAACGACGGCGTGAACGCCCCAACCTTCCACGTACAGTGACCCAGCTAGTGGCCGAGGATG

GGAGCAGGGTGTATGTGGTGGGCACTGCTCACTTCAGTGATGATAGCAAGAGAGATGTAGTAAAGACTATCCGGGAGGTG

CAACCGGATGTGGTCGTGGNGGAGCTCTGTCAGTACCGGGGTGTCCATGCTCAAGATGGACGAGAGGACGCTGCTGCGAG

AGGCCATGGAGGTCAGCCTGGAGAAGCTGCAGCAGGCTGTCAGGCAGAATGGNTTATGTCTGGACTCATGAGATGTTGCT

GCNTGNAGGTGTCTGCTCACATCACTGAGCAGCTGGGCATGGCCCCTGGTGNCGAGTTCAGGGAGGCCTTCAAAGAGGCC

AGCAAGGTACCATTCTGCAAATTCCACCTGGGNGACCGACCAATCCCAGNC

Clone 0115-T7 sequence

AGCCCGTCCCCAGCGCTCCGGCGAGCTGGCCTCCGCTGCAGTTGAGCCGGGCTCGCACTATGCAGCGCCTGGCCATGGAC

CTGCGGGTGCTGTCCCGGGAGCTGGCGCTCTACCTGGAGCACCAGGTTCGCGTCGGCTTCTTCGGCTCGGGCGTGGGCTT

GTCACTGATCTTGGGATTCAGCGTTGCCTATGCTTGCTACTACCTGAGTAGCATTGCCAAGAAACCCCAGTTAGTGATTG

GAGGGGAGAGTTTCAGCNCGCTTCCTACAGGACCACTGTCCCGTGGTGACAGAAACCTATTACCCGACGGTCTGGTGCTG

GGAGAGCCGAGGACAGACATTGCTGAGACCCTTCÄTCACTTCCAAACCCCCGGTGCAATACAGGAACGAACTCATTAAAA

CTGCAGACGGAGGACAGATCTCACTGGACTGGTTTGATAACAATAACAGTGCGTACTATGNGGATGCCAGCACCAGACCN

TACTATCTTGCTCTTGCCTGGCCTNACTGNAACAAGCAAGGAATCCTACATCCTTCATATGATCCATCTCAGTGAAGAAT

TNGGATACAGGTGNGNGGNTTTTAATAACANAGGAGTAGCANGAGAAAGTCTCTTGACACCACCGGACTTACTGCTGNGC

GAACACTGAAGAACTTGGAGGCCGNNGTCCACCACGTGCACAGCCTGT

Clone 0116 contig

AGGGCGCAGCCGGCCTAGCGAGGTCAACATGCCGGTCGCCAGAAGCTGGGTTTGTCGCAAAACCTATGTGACCCCACGGA

GACCCTTCGAGAAGTCGCGTCTCGACCAGGAGCTAAAGTTGATTGGAGAGTATGGACTCCGGAACAAACGTGAGGTTTGG

AGGGTCAAGTTTACCCTGGCCAAGATCCGTAAGGCGGCCCGGGAGCTGTTGACGCTGGACGAGAAGGATCCCCGGCGTCT

GTTTGAAGGCAATGCTCTCCTGCGGCGGCTTGTTCGCATTGGGGTGCTGGACGAGGGCAAGATGAAGCTGGATTACATCC

TGGGACTGAAGATTGAGGATTTCTTGGAGAGGCGGCTGCAGACCCAGGTCTTTAAGCTGGGCCTGGCCAAATCTATTCAC

CATGCCCGTGTGCTCATCCGCCAACGTCACATTAGGGTCCGCAAGCAGGTGGTGAACATCCCATCCTTTATTGTTCGCCT

GGACTCGCAGAAGCACATCGACTTCTCCCTCCGTTCTCCTTATGGTGGCGGCCGTCCAGGCCGAGTGAAGAGGAAGAATG

CCAAGAAAGGCCAGGGCGGGGCTGGAGCTGGTGATGATGAGGAAGAGGATTAATTCTTGGCTGAACTGGAGGATTGTCTA

GTTTTCCAGCTGAAAAATAAAAAAGAATTGATACTTGGAAAAAAAAAAAAAAAAAAAA

Clone 0117-T7 sequence

AGCGGCGGCTCTGAGTCGCCGGAGCCGCCCCGGGACÄTCATGGCGGAGGTCTGCGAGCCCACCCGCCCCTCGGAGGACGA

GGACGAGGAGCGGGAGCCGCTGCTGCCTCGCGTTGCCTGGGCCCAGCCGCGGAGGGTCGCGCCCGGGAGCGCCGTGAGAA

TGCAGGCGGACGAGGGCGCGGATGTCCTCCGCGAGCCCGCTACCGACGAGCCGCCGGCGGTGTCCGGGGAAGGGTCGATC

TCCGCGAGCTTGTCCACCGAGCTGGATGGGACCCGCACTACGAGCTCAGAAACAAATACATTCTTGGAAGATCCAGAATT

TGCTGATATTGTATTGAAAGCAGAGCAAGCAATAGAAATTGGAGTTTTCCCAGAAAGAATTTCT

Clone 0119 contig

CATCATCGAGACAAGCACCATTAAGCCTTACCGTCGAGGGTTTTACTGCAATGACGAGAGCATCAAGTATCCCCTGAAAG

TCAGTGAGACTATAAACGATGCTGNGCTCTGNGCGGNGGGGATCGTCATCGCCATCCTGGCGATCATTACAGGGGAATTC

TACCGGATCTATTACCTCAAGGAGAAGTCCCGCTCCACCACTCAGAACCCGTATGTGGCAGCNCNCNATAAGCAAGTGGN

ATGCNTCCTTTNNGNTGTGCCAATTAGCAAGTCCTTCANNGNCATCGCCAAAGTGTCCATCGGGCGCCTAAGGCCTCACT

TCCTNAGCGTCTGTGACCCTGATTTCAGTCAGATCAATTGCTCCGAGGGCTACATTCAGANCTACAGGTGCAGAGGAGAA

GNCAGCANAGTACAGGAGGCCAGGAAGTCCTTCTTCTCGGGCCACGCCTCCTTCTCCATGTTCACTATGCTGTATCTGGT

GCTCTACCTTCAGGCCCGCTTCACCTGGCGCGGGGCCCGACTGCTCCGCCCCCTCCTGCAGTTCACTTTGCTCATGATGG

CCTTCTACACGGGATTGTCACGGGTATCTGACTACAAGCATCATCCTAGCGATGTCCTGGCAGGATTTGCCCAAGGAGCT

CTGGTGGCCTGCTGCATAGTGTTCTTCGTGTCCGACCTCTTCAAGACTAAGACGAGCCTCTCACTGCCCGCCCCTGCGAT

CAGGAGGGAGATCCTGTCTCCCGTGGACATCATCGACAGGÄACAATCACCATAACATGGTGTAGATGCTGCGGCCTCCGG

AGCGCTTTCTCTGAAGCGACTGCACGTTCCTGCTGCTCTCCGATCTCATCAGACAGTAGAATGTAGGGAAAAGCTTTTGC

CCGATGGATTTTGAAAACATTTAAAAAAAAAAAAAAAAA

Clone 0121 BGH sequence

TGCAGGGAAGACAGAAAGGCCTCCCAGGCCACTTGGTTTATTAGATCCTGAAGAGAGGTGTAGGCAGTGCCCCTGGGCCG CTGCCACCTCCTGGGGGAGGACCTGTGGGAGGCACAGGGCCGAACCTCGTTTTGATACACACAACCCCATTTGAGGGAAA ACAGGCTGCTTCGAAGCCTGAGGGATGGTGAGGGGTGATGCCTGCCATACAGGAAGCCAGGTCCTGGGAGGGCACAAACG ATGAATCCATCACTGCCCCAGCTCTGCCAGCATGCCCACCTGGCCCTGGGGAAGCCAGGCAΆGGGAGGGCACAGGCGTGT GAGGGACACAGACAGTTCCTGGTGACGGCAGTAGCTGCTGAGCAGGAGGGTTCAGCAAAACTGACCATTAGAGCAGCCAA GGCTGCATATAGGAGGTGCGCTCGGGAACCCCAGGCACTTTCTCTGGACTCCACGGTCATGGCTTCTGCTGGTGATCTGC ACTGCCTGCCTGTCCCCTCTCCTGAAGGCACTACCTTCCAGAACACAGCACGGTGGTCCCTCTTGTGACAAAGTGCTTGT GTGTGTGCTCTAGTCATTTGGTAΆGCAGAAGCTGCCACGGGCCATACCCTGCCACACTACCCAAGTTCTGGGCNGGAAAC TGCTCCCTGCACAGAGGGCCAGCGGGAGCAGGAAACGAACTCAACTTCGCTGGGCTTGCCAGGCANGGCACGGTAGCAGC CAGGGAGGTTGGGACAGTGACÄNCAGNCAGGCAGACTTTTTTG

Clone 0123-T7 sequence

AGCAACCGCAACCTGACCTTGACCAAGAAGGAACCTGTTGGGGTCTGTGGTATTGTCATCCCCTGGAACTATCCCTTAAT GATGCTGTCCTGGAAGACAGCAGCCTGCCTGGCTGCCGGG ACACCGTGKTGATCAΆGCCTGCCCAGGTGACCCCACTCA CAGCCTTGAAGTTTGCAGAGCTGACACTGAAGGCTGGCATTCCCAAGGGTGTGGTCAACATCCTCCCAGGATCTGGCTCG CTGGTTGGCCAGAGACTCTCAGACCACCCTGATGTGAGGAAAATAGGGTTCACAGGCTCCACGGAGGTGGGAAAACACAT CATGAAAAGCTGTGCCCTGAGTAATGTGAAGAΆGGTCTCCCTGGAGCTGGGTGGAAAGTCACCCCTTATCATCTTTGCTG ACTGTGACCTCAACAAAGCTG

Clone 0124 contig

TGGAAAGGCGATGGGCATCTCTCGGGACAACTGGCACAAGCGCCGCAAGACCGGGGGTAAGCGAAAACCCTACCACAAGA AGCGAAAGTATGAGCTGGGACGGCCCGCTGCCAACACGAAGATTGGCCCTCGCCGCATACACACAGTCCGAGTTCGAGGA GGCAATAAGAAGTACCGTGCCCTGAGATTGGATGTGGGGAACTTTTCCTGGGGCTCTGAGTGTTGTACTCGCAAAACAAG GATCATTGATGTTGTCTACAATGCATCCAACAACGAGCTTGTCCGCACCAAGACCCTGGTGAAGAACTGCATCGTGCTTA TTGACAGCACGCCGTACCGACAGTGGTACGAGTCCCACTATGCACTGCCCCTGGGCCGCAAGAAGGGGGCCAAGCTGACT CCTGAGGAGGAAGAGATTTTAACAAAAAACGATCAAAGAAAATTCAAAAGAAAT CGATGAAAGGAAAAAGAATGCCAΆ ATCAGCAGCCTCCTGGAGGAGCAGTTCCAGCAGGGCAAGCTTYTAGCCTGTATTGCCTCMCGACCAGGCCAGTGTGGCA GAGCAGACGGCTΆTGTGCTCGAAGGCAAGGAGCTGGAGTTCTATYTGCGGAAGATCAAAGCCCGGAAAGGCAATGAGCC TCATGTGTAGTGTAATAAAGGTGTYTGCTGTTCTATYTTAAAAAAAAAAAAAAAAAAAAAAA

Clone 0125 contig

CTGGAAAGCCHGGATGAGGCTCTTCATCGCTCTTCCTGTCCTGATTGTGGTCGTAGCCATGACCTTGGAAGGCCCAGCCC

CCGCCCAGGCGGCCCCGGATTTGTCCGGAACATTGGAGAGCATACCGGATAÄACTGAAGGAGTTTGGGAACACTTTGGAA

GACAAGGCCCGGGCAGCCATTGAACATATCAAACAGAAGGAAATTTTGACCAAGACCCGGGCCTGGTTCTCAGAGGCATT

TGGCAAAGTGAAGGAGAAGTTGAAGACCACGTTCTCCTGAGCACCTGGCGGGCCACCTCGAAGCATCAAGGACATCCACG

TAATGTGCCAGGTCCCTCTTCATCACAGACCAATAAAAAACGTGTAGAAGGCAAAA

Clone 0127 contig

AGGTTTGAACAGCTTTCTGAATCTGCAAAAGAAGAGCTGATAAACTTCAAACGGAAGAGAGTGGCAGCATTTCGAAAGAA

CCTAATCGAAATGTCTGAACTGGAAATAAAGCATGCCAGAAACAACGTCTCCCTGTTGCAGAGCTGCATCGACTTATTCA

AGAACAACTGACCTGTCTAYTTTAAAGGCCCCCAWTGTGAAAGCCAGCATCACTTGCACTTAAATCATTACTGCAAAAGA

AATAGCTTTGACTAGTTTAAAATCATGTGAATAAATATTTTGATTTCTAAAAATCTTAACACTTAACCATGTTGGTTTAA

AAATATTTTCATTGCATGTTACTTGGACATAACTAATCCTTTTCTTATGCATTTAATACCTCAAAGTAGGTAGAATCTAA

CAGATTCTCCTATAGACTGTCAGGAATTACTAAGAAATGGTATGGANATGTGTGCCTTCTGGAAATGAGAAGCAATAGCC

TGGCCCATGTCTGTCTGAACATTTCCTGTGTCTGCTCCTACAGACATGTCAGGTGTGAATTACAGCAAACAGTGACTGCT

TGTGGGCAGGTGGCAGACACTCTTCTTTATTTAGGTANATTTTGCTCCCTCTTGTCAAGATCACTTCANATGCTTGTACG

GTAACAAGCAAGAAGATCTCATATGTCTTTTTACATCCTTTACAGCTCCATTTTTGTAACTGGGATATAAATACTTGAGA

GAAAGGAGAATCTANAGTTNTGACACTCCCTGATGGTGGGGCATGTGTGTTTGTGCTTTGGATGTTTTTATAAAGTATAT

AATAAAGATAATCCATGTT

Clone 0128-T7 sequence

GGCGTGCTGACGTCAGGCGCGTGCCCCTGTCCGGCAGCCGAGGAGGCCCCGCGCAGTGCTGCCAACGCCCCGGCGGAGAA GCTGAGGACAAGATCTAATTTGAAATATTAAAAGTGGATACAAAACTGTTTCCGAAATGCAGACAATTAAGTGTGTTGTT GTTGGTGATGGTGCTGTTGGTAAAACATGTCTCCTGATATCCTACACAACAAACAAATTCCCATCGGAATATGTACCAAC TGTTTTTGACAACTATGCAGTCACAGTTATGATTGGTGGAGAGCCATACACTCTTGGACTTTTTGATACTGCAGGGCAAG AGGATTATGACAGACTACGACCGCTAAGTTATCCACAGACAGATGTTTTTCTAGTATGTTTCTCAGTGGTCTCTCCATCC TCATTTGAAAATGTGAAAGAAAAGTGGGTGCCTGAGATAACTCACCACTGTCCAAΆGACTCCTTTCTTGCTTGTTGGGAC CCAAATTGATCTCAGAGATGACCCCTCTACTATTGAGAAACTTGCCAAGACAAACAGAAGC

Clone 0131-T7 sequence AGAGGGAGGCTCTCAGCGATACGTGCCCGGCCCAGCTTTTTTTTTCTTCAAAATGTCTACTGTCCACGAAATCCTGTGCA AGCTCAGCCTGGAGGGTGATCATTCTACACCCCCAAGTGCCTACGGGTCAGTCAAACCCTACACCAACTTCGATGCTGAG AGGGATGCTCTGAACATTGAGACAGCAGTCAAGACCAAAGGAGTGGATGAGGTCACCATTGTCAACATCCTGACAAACCG CAGCAATGTGCAGAGGCAGGACATTGCCTTCGCCTATCAGAGAAGGACCAAAAAGGAGCTCCCGTCAGCGCTGAAGTCAG CCTTATCTGGCCACCTGGAGACGGTGATTTTGGGCCTATTGAAGACACCTGCCCΆGTATGATGCTTCGGAACTΆAΆAGCT TCCATGAAGGGCCTGGGGACTGACGAGGACTCCCTCATTGAGATCATCTGCTCACGAACCAACCAGGAGCTGCAAGAGAT CAACAGAGTGTACAAGGAAATGTACAAGACTGATCTGGAGAΆGGACATCATCTCTGACACATCTGGAGACTTCCGAAAGC TGATGGTCGCCCT

Clone 0133 contig

CTGGAAAGGCTCCTCGAGGGTCGGTCCCGNCACGTCTNTTCCCGGTCTCTCTGGCTCTGGAGTGCTTTCCCCCCGCGGAG

GGTGGGAGCGCGGGGACAGACGGGCGAGATGAGCACCATGTTTGCAGACACGCTGCTCATCGNCTTTATCTCCGTGTGCA

CCGCGCTGCTCGCCGAGGGCATAACCTGGGTCCTGGTTTACAGGACAGACAAATACAAGAGATTGAAGGCAGAAGTGGAA

AAACARAGTRAAAAATTANAGAAGAAGAAGGAAACTATAACAGAGTCAGCTGGTCGACAACAGAAGAAGAAGATAGAGAG

ACAAGAAGAGAAATTAAAGAACAACAATAGAGACCTGTCAATGGTACGGATGAAATCCATGTTCGCGATTGGCTTTTG T

TTACTGCCTTAATGGGAATGTTTAATTCCATATTTGATGGTAGAGTGGTGGCAAAGCTCCCCTTCACTCCTCTGTCTTAC

ATCCAAGGACTATCTCATCGGAACCTGCTGGGAGATGACACCACGGACTGCTCCTTCATCTTCCTGTATATCCTCTGTAC

CATGTCAATTCGACAAAACATCCAGAAGATTCTTGGCCTCGCCCCTTCACGAGCTGCCACCAAGCAGGCTGGTGGATTTC

TTTGGCCCACCACCTCCGTCTGGGAAGTTTTCCTGAAGGAAAGCAGAATTCTGAATTTCCTGTCATACTTTTTAGACATT

CACATCAGACTTACAGAGCACCTGGCCACATTCTAGGTTTGTGTTATTTCTCAGCTATGGATATGATCAAATGAGANCAC

TGCTTAACTAATGGGATAGTGCTATGGTCACGGATGGCTTCTTTAGTAATAAGTGCACTCACTGGTTACATTTGGATGGC

TTATGTAACCACAAACATCATCTTTCTTGTCTCACTTGAGTATTTCAGTGTATGTGCCATATACACAGAAGCAGACTGTC

CATGAACATTAGGAGAAAAATAGTTTATGTAACAACTTCACTGTAATTAGTTGTTGTGTTTTTTAAAAAGCCAAATATGT

CACTTTGTGTTCGACAATGAGCCAAATTTTTAGATGTTTCAGTTAGAGAAATGTTTGATTTTTTTGCATATTAAAAAGAG

TATTTTCTGTAATCTGAACATAΆGGATAAGCATTGGCCCAAAAAGCTAAAATAAAGTTTTGTCATGTTCTTTT

Clone 0134 contig

ANCCTCTTCTTGGGNCTAACCTAAAAAAAACCCCCCCCTGGTTTACCTGGGCTTTTTCGGAAATTTNATTNCGGNCCCCC CCTNTTAAGGGAGNCCCCNAAGCTTTGGGTNCCCAAAGCTCGGGATTCCCCTTATTTACCGGCCCGCCCCATTGTGCTGG AAAAGGTGGAASCCSCCGCCACGATGCAGATTCTTTGTGAAGACCCCTTMCGGGGAAAACCCWTCACGCTCGAGGTTGAA CCCTCGGACACTATAGAAAATGTAAAGGCCAAGATCCAGGATAAGGAAGGAATTCCTCCTGATCAGCARAGGCTGATCTT TGCTGGTAAGCAGCTGGAAGATGGCCGGACTTTGTCTGACTACAACATTCAAAAGGAGTCCACCCTTCATCTGGTGTTGA GACTTCGGGGTGGTGCTAAGAAAAGGAAGAAGAAGTCTTACACCACTCCCAAGAAGAMCAAGCATAAGAGGAAGAAGGTT AAGTTGGCTGTGCTGAAATACTATAAGGTGGATGAAAATGGCAAAATTAGCCGACTTCGTCGAGAGTGTCCTTCTGATGA ATGTGGTGCTGGAGTTTTCATGGGAAGCCACTTTGACAGGCATTACTGTGGCAAGTGTTGTCTGACTTACTGCTTTCAAC AAACCAGAAGACAAGTAGTTGTGTATGCGTTAATAAAAAGAAGGAACTCGTAAAAΆAAAAAAAAAAA

Clone 0136 BGH sequence

TTTTTTTTTTTTTTTTTTTTTTTTTTTGAAAATTAAATCGAGATATTTAATAGACGGKGCAAACCATAATTTCATCTGAA TATAΆATGTATGCACACGTTTCCAATGAGCTATGTATCAATTATCACAACAGCAATGAGGACCTTAGAGCTACGTTAGAA AACTGGATAGCTGGAATTAACTCTAACAATCTTATTCGCTTGCAAGAGAACACATCCATTTCAGATCCGTGTCACAAACT CCAAAAGACCAGGGGGGACAGGAGTGACAAGGGCCAGGATGGTAGACATGGGGCCAGTACACAGGTGACAGACAGAAACA CAGCTCTTGAGGGAACCAAAACTAGATGTGTTATTACAGCTAAGAACAAGGAAAAGCCTATCTATGGCTTTTAAAAAACG ACTTTTCTGCCAGTACCGTACACATCTTACAGAGTGAGACACGTCAGCTGCAGGGCTACATTTCCAGTTCGCTTCAACTG CAAACGGCCGACCCTCCACAGAGGACGCAGCAGTGGGCTTAGCGCAGCCCAGCAACTCAGTGTTGGATTTGCAAATACAG TTTATTTTACAGGAAATTCAACATTATAAAMNGGGGAACT

Clone 0137-T7 sequence

AGCAAGGACCCTGAAAATAAACAGCCGCTGCTTTGCGAGTCGCCTTCTTGGTTCTTCGTCCGAGTCTCCTCCGCTGTGGG

CAGCTCAGACGCCGAAGCTCTAÄCTGCAGCCTATGAGCAGCAACGAATGCTTCAAGTGTGGACGATCTGGCCACTGGGCC

AGGGAGTGCCCTACTGGTGGAGGTCGGGGTCGTGGAATGAGAAGCCGCGGCAGAGGGTTCCAGTTTGTTTCCTCGTCTCT

CCCTGACATCTGCTACCGCTGTGGTGAGTCTGGTCATCTTGCCAAGGATTGTGATCTGCAGGAGGATGCCTGCTATAACT

GCGGTAGAGGTGGCCACATTGCCAAGGACTGCAAGGAGCCCAAGAGAGAGCGAGAGCAATGCTGCTACAATTGTGGCAAG

CCAGGCCATCTGGCTCGTGACTGTGACCACGCGGATGAGCAGAAGTGCTATTCTTGTGGTGAATTTGGACATATTCAAAA

AGACTGCACCAAGGTGAAGTGCTATAGGTGTGGTGAAACTGGTCATGT GCCATCAATTGCAGCAAGACAAGTGAAGTCA

ACTGTTACCGCTG

Clone 0137 BGH sequence TTTTTTTTTTTTTTTTTTCAGTCCTCCACATTCCCCTCTTTAATATGGWATTTTGTTCTATATACATTGATGAAAATTTA AAACAAACAAAAGGAACTTTAGTCAAAACTCCCCTTCCTCCTCCAGCTTTATTGGAATAGTTTAAAATTACATTTTCTAT TTTCTAGGACTTCAGTTGCTTTTTCCATCTGACTTTTAAAAACTGATTACAGCAAATGAAACACAGTTGTCTAAGTGATA TAGTATACAAAAATAACATCTTGATTTCTGTGAAAATGCATTTCTCTGCAATTCCTGAATAGCTCCAAATTATGCTAACT CTGAGCATCGGATGTTTACTCTGGGTTTTAGATTTAGTCTTTGAAAAAATGTGTTCTACACCTTTGCCATCACCATCTGT ACGTCCAGCATCGTCACTGGGATGAAGCTGCTTCTGTGTTAGGCTTTGAGCCAGATTCCCTGGCATAGCCTTCAACACAT TTGTCTTTTCAGTTTACTCCCAACTCAACTGTATATTCTGAGGGCCTGATGCAGATGTTACTCGATGTTATTTAATAGCT ACTGAGGGTCAGACAATCCAAGGCCGNCATTATGCTAANAÄTTAAAGGNTATTTATGGAAGCTTAAATGCTTNCAGAATT GGNTTTACCTTCTACATGGGGAACACACT

Clone 0138 BGH sequence

TTTTTTTTTTTTTTTTTCGCATATAAATGATTCTTTATGCCCTTCCCCCATGCAGDGGGGGGCGGAGCAGGGGGAAGTCC

CCCGCCTCATGCAGGGAACGGGAGGTCAATGTTTAGTAGTCTTATTGCCAGATGCCCTGTGGCCATGGGGTGGGGAGTGG

GGGGTGTCWACAGCCCCTTGTTTTCTGGCATTGGTCTCCCTTTACCAAGTCACCATGGTGGAGCCAGGGGACTGGAGGCT

TAAAGCTGAGGTAAAGATGGTTCTCTCCAGGAAGAAAAGTGGTTCTCCCCGCTTGCCTGCTGGCCATCCTCAAGGTGAGT

ATGAGGCGGNGTCTGTGGGAANTGGAACGTCACCCAGGGGTCTCTACCCTGTGGNCAGTANGCCGGACAATGTCTC

Clone 0139-T7 sequence

AGGGACCCCGCATCTTATTAKCAACCAGGGAGATTTCTCCATTTTCCTCTTGTACTACAGTGCGGCCTACAAATCTGGGA

TTTTTTTTTATTACTTCTTTTTTTAAAAAAACTACACT GGGCTCCTTTTTTGTGCTCGACTTTTCCACCTTTTTCCCTC

CTTCCTGCGCTGCTGCTTTTTTGATCTCTTCGACTAAAAATTTTTTATCCGGAGTATTTAATCGGGTCTCTTCTGTCCTC

CTCGCCACCCCCACCCCCTCCCTCCGGTGTGTGTGCCGCCGCCGCTGNATGCTGCTGCTGCTGCTCGCCCCGTCGTTACA

CCAACCGAAGGCTCTTTGTTTCCTCTCTTGGATCTGCCGAGTTTCTTTGTTGAAGAAGCCAGCATGGGTGCCCAGNTCTC

NAAGACCGCAGCGAAGGGAGAAGCCACCGTCGAGÄGGCCCCG

Clone 0139 BGH sequence

TTTTTTTTTTTTTTTAAATCTGGGTATTGGTGTTTTTATTTTTTTACTCTTTCCTTCTTATTCAAAACGTGTAGTGTTGT

AAACCTGCCTCACAAAATACATCGTAATAATTTTTCTTTAAAAAAAAAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA

AAGAAAGAAAGGACAAGAACCCTAAGCCTTTACACCCACCACCATTTTAGTGGCATATTATCTTGGCAATGTTATGCACT

GCTTCAATTTCCCATAGTGGCCCCTATCACTTCATTTGTTATTCCTTTTGACCCACCCATCTCCTTCATATATGGGTATG

TCTATAGATCTGACAAAGAAAGTTTACACTTTTTTAATAAAGATGCAAAGTATGCAAAAAACATTAATACTGATGCCAAA AAAAAAAAAAAAAAAGTAAAAGTAAAGAAAGAAGGCAGAGGAAGCTGTCTAACACGTCCTCGGCCTGTCGGAATGGTGGA AACAACTATCTGAGATGGGATCTGTGGGGAAGGGGGCTTAAAAAAAACANAAAACAAAAAATTTGCTGNTTAANAΆAAAT GTGAAATTTAAAANANAATNTTTAAAATNAATCCCTGGTTGTAGACANGTTNCCAAAAACCAGTACCTGGCACCACTCCA

AC

Clone 0140-Contig CTGGAAAGCTCGGTTCGGTGTCTGGGGTATGAAGGTCCTGCAAACCCGAGGCTTCGTCTCGGACTCGTCGGATAGCATGG

ATACGGGCGCTGGCTCCATCCGAGAAGCTGGTGGAGCCTTCGGAΆAACGAGAAAAGGCTGAAGAGGATCGGTACTTCCGA GAGAAGACTAAAGAACAGCTGGCTGCCCTGAGGAAACACCATGAAGATGAGATTGACCACCATTCGAAGGAGATAGAGCG TCTGCAGAAGCAAATTGAACGCCATAAGAAGAAGATCCAACAACTAAAGAATAATCATTGAATGCGCGCAGTCGGTCCCT CACAGAGTGGCCCGTATCACTCCCCACGTCTGTAGACACATGGCTTTGAATGATTACTATTTGGTCTGTGTGCTACTAAC AGATAATAAACGATCACCAGGAAAAAAAAAAAAAAAAA

Clone 0141 contig

AGGGAGGTAGAGGTGMCCGATCCTGGGGGTCGGTGTTAGGCCGTGGGAGGTTTTTCGCGAGACTGAGCCACGCGTCTATC TTCTGTCCCAGGGCCGCCGTCACCATGGGCCGCGAGTTTGGGAΆCCTGGCGCGGATACGGCACGTGATCTCCTACAGCTT GTCGCCCTTTGAGCAGCGCGCCTTCCCAAGCTATTTCAGCAAAGGCATCCCCAACGTGCTGCGCCGCACTCGCGAGCGCA TCCTGCGCGTGGCGCCGCCGTTTGTAGTGGTCTACCTGATCTACACATGGGGCAACCAGGAGTTTGAGCAGTCGAAAAGG AG _A _A A _T G _AAA CCAGCC _ATGTAT ATGACAAGTAGACGGCCTGCACCTGGGTGACAGTCCCCTGCCTCTGAAAGACCCTTCT CTGGGAGAGGA _AT CC C C _T _M GTAGTCTTGAAGMCAATAAACTACTTATGGMCTTCCAAAAAAAAAAAAAAAAAAAAAA

Clone 0142-T7 sequence

_{CGTTGTCTTGCTCCGGCTGCGCG} CATTGTCC CAGGGTCCTCCGACAGGGCTGCTGCGGGGCCCGGGACCCGCGCCCTAG _T _GG A C _CGCGCCC _CGCTGCCGGTCG GCCTGGCGCGGGGCTCTGCTAGTCTGTTGGCGAGCCCGTGC _TA CCGGGCTAGTCTCG CCGGG _GTT TT CC _T G _A _TGCGAAGTT GGAAGGGGAGAAG CCCACCCGTCCGCCCAGCCC _T _A _T GCC TNCCCGGCGCGCAGCCT CG _A CG _GG GCCGTG _G _AG C GC _GC GACGAGGGCCGCCGACGGAGCCCTGAGAGAAGTACAAAGTGGTTNGTAACTGGGCTCGN Clone 0142 BGH sequence

TTTTTTTTTTTTTTTCAGGTTTGAAACTTTTGCTTGCCGGTGTCΆAGCAGCCAATACAGAGCCTTAATGGCCTATTTAAA CGGGGAAATATGCATGAACCAAAATCAACGAGGAACGTACACGCTGTATAGAGCCATTTTTCCACCGGGGTCTCAGATGC TCTGCAACGTACACCCTCTCAGACCCTGGCTGAAGGCTGGGGATGCTGGCTACACAAGTCAGCCAGGTTCCTCGGACATC CCAGCCAAGACAACAAAGTCGTCTCCAATCTCAAΆGCAAGAAGACAATCACATTAAGAGAATCGGGTGCGTTTGGCTTTG TGCACAGAGCGTGGCCAGCGCCCGGCGGCAGA ^CG G ^CGCCTC ACAGGATCACG ^C AGGCCGAGCAGCAGCCTCGTCGCCGTT GGGCT ^G TGCC ^GCG TAGTT ^C ATCTGCCTCAC ^G AT ^C T ^CTGCG AATAGCTCATCCA ^C TGAGGCTTTGTTTTTGGCCCGATGTC TCCATGAAGGGGCAGCTCCACTCCTCGGCCAGG ^G C ^C TTA ^CC CTCGCCATAGGAGACCTCACGTTCACCCTCCAAGTCCAC CTT ^G TT ^GCC T ^ACCAGG AT ^CATG G ^GG TA ^{CGCGCTCGTACCGCT} T ^CACGCG GAT ^GA T ^C TGGG ^C CCGCATGGGCTTGATGN

Clone 0143-T7 sequence

TGGNAAGGGGGGACTTTTGGTATAGCATTGGAAATGTAAATGAGCTAAATACCTAATAAAAAATGGAAAAAAAAAAAAAA AAAAAAAAAAAGAAMTAGAAAAAATGTTTACT ^G AG ^G T T ^T ^CT ΆTGT ^C TGTTGTTTTGTTTTGTTTTAGAAGTTTCTGAACG TTGTTTTAGGACTAAACTGGGAAGCCATGTGTCTTCGTTTTGGATTTTATAGATAACCTGAAGCATTATTGAGATTCCTT CAAAGTCAGTAACAACTTATTGGGTGTTTCAAGCCAGTTGTGAGTCTCTGAGTATTTGCCCAGNTTCACATTTATTGGTA AAGGTCCCTTACAAG

Clone 0143 BGH sequence

_{TTTTTTTTTTTTTTTGACTCTTGCTACTTGTGAGGCTCAGCAAAAAGAATTTTTTTTTTTTTTTTGACTCTTGCTACTTGTGAGGCTCAGCAAAAAGAAT}

ACTATAGATGGAATCAGTCTGTACCACTCTGTGCCATTGTCCTCTGAGTAGGTTCTCTGTGACTGTAGATTATTTTTCCA GTCTATAAAAGGTCATGGGTGCTATTGGAACTCTAGTCAGGCATCAACTTTAGGCCATCATTTCTCCATCAAAGGAAAGA GAGCTTCTACATTTCGCAGACÄTGAGCATCCTAGATTACCCATAGTGCTCAAGTGTCATTTGAGTAATTTATTGAGATAG CAAAGGCCTTTTGCATCTGATCATGCTGGGAAGAATGGAGTAAGTTTGGCATCCTTACACCTACCGAGTTGGCGACAGAC ATATTCCTCAGGAGGTTGGAATGAAATTTTTGAATGTTCAGAGGAAACCTATATACATTTAAGGTGAAATCTAAAGAATA AGAGAGAAAAACAAAACAAAACAACAAAAGAAACTCTTACCATTTGAGCACTAAGAATAGTCAAAGCAGAGGAAACAGGC TAGTGCAAAGGTCCATCTAGTACAAGAGAGNTTATGGCTTGCTCCAGGAACGGACAGAAGGCTAGCGNGGCTGGCG

Clone 0144 contig

AGGGACAGCTCCCCTTTCTGGAAGGTGAAATTTTTGATTCTGTGAAGCCGGGACTTTCTGCTTTTGTGGATCAGCCCAAA

CAGGGTGCTGAGACTGTCCAGGAGCTCTTGGAGGTGGCCAAAGACTCGATCCCCAGAAGCCACTGGGAAAGGACCCCGGT

GGTTCTGAAAGCAACGGCCGGACTCCGTTTGCTGCCTGAGCAGAAAGCCCAGGCTCTGCTCTTGGAGGTAGAGGAGATCT

TCAAGAATTCACCTTTCCTGGTCCCAGATGGCAGCGTTAGCATCATGGATGGGTCCTATGAAGGCATACTAGCCTGGGTT

ACCGTGAACTTTCTAACAGGTCAGCTGCATGGTCGTGGCCAGGAGACTGTGGGGACCCTTGACCTGGGGGGTGCCTCCAC

CCAAATCACGTTTCTACCCCAGTTTGAGAAAACCCTGGAACAAACACCTAGGGGCTACCCTCACTTCCTTTGAGATGTTT

AACAGCACTTTTAAGCTCTATACACATAGTTACTTGGGATTTGGACTGAAAGCTGCAAGACTGGCÄACTCTGGGAGCCCT

GGAAGCAAAAGGGACTGATGGACATACGTTTCGAAGTGCCTGTTTACCAAGATGGTTGGAAGCAGAGTGGATCTTTGGGG

GTGTGAAATACCAGTATGGTGGTAACCAAGAAGGGGAGATGGGCTTTGAACCCTGCTATGCGGAAGTGCTGAGGGTAGTA

CAGGGGAAACTTCACCAGCCAGAAGAÄGTCCGAGGAAGCGCCTTCTACGCTTTCTCTTACTACTACGATCGAGCCGCTGA

CACACACTTGATCGATTATAAAAA

Clone 0145 contig

AAAAGGGCAGCCATGGCGCCCAGCCGGAATGGCATGATACTGAAGCCCCACTTCCACAAGGATTGGCAGCAGCGAGTGGA CACTTGGTTCAACCAGCCGGCGCGCAAGATCCGCAGGCGCAAGGCCCGGCAGGCGAAAGCGCGTCGCATCGCCCCTCGCC CCGCGTCCGGCCCCATCAGGCCCATCGTGAGGTGCCCTACAGTGAGATACCACACCAAGGTCCGGGCTGGCAGGGGCTTC AGCCTGGAGGAGCTCAGGGTGGCTGGCATCCACAAGAAAGTGGCTCGCACCATCGGCATCTCTGTGGACCCGAGGAGGCG AAACAAGTCCACGGAGTCACTGCAGGCCAACGTGCAGCGCCTGAAGGAGTACCGCTCCAAGCTCATCCTGTTCCCCAGGA AGCCTTCTGCTCCGAAGAAGGGAGACAGTTCTGCTGAAGAACTTAAGCTGGCCACCCAGCTGACAGGACCTGTGATGCCC ATCCGGAATGTGTACAAAAAGGAAAAGGCCAGAGTTATCACAGAAGAAGAGAAGAACTTTAAGGCTTTTGCCAGTCTCCG AΆTGGCCCGTGCCAATGCCCGACTCTTTGGCATCCGAGCAAΆAAGGGCGAAGGAAGCTGCAGAGCAAGATGTTGAAAAGA AAAAATAATTCTGTGTTGGAGAGCTGCAATAAATTTTCCATAAAGCAAAAAAAAAAAAAAAAAAA

Clone 0146-Contlg

_AG GGCGA _A GAGTGAGTGCCAAGGTTCATATGGGAAGGACTTTGGGGTGAGCATCTTCTCTA _TTT CC _AGC TGGCTTTTCTG A _TT TTCAG _A AAG _AA G _A C _T CATCAAAGATGTCCAGCAAGACGGCAAGCACC _AA CAGCATAG _C CC _AA GC _CA GGAGAACTGTG _CA GCAGC _T GAGATTGG _AΆ GCCTCCATCGAAAGAATAAAGGTCTCAAAAGCA _T CAGC _A G _AC C _TGATGTCATA C _T G _T GAGGA _GCA TGCCCGGAGCGACCCTCTGCTGATGGGCATÄCCGACCTCAGAAAACCCGTTC _AA GGAT _AÄGAA GACC _T GC _AT CATCT _T ATAGTGG _A CCAGG _AA GCGCCCCT _T GCCTCTTAACGCAAACCACAGCAGC _A ACCTGAAGG _GATT CC _TTC AGCTT _A CCTGG _T AACCACAGCTAGTAACTAAAACACCCTTCTCTCGGAATAATAGACCCTGAAGTCTCTCTCTTTTT CACCCTTTACTGATTTAATACAGAATAGCAATCTTATTTTCTATTTGATAACTATGGTATCATATTGGGTTACTGTATAA GGAAAATGGCAGGGGAGTTGTGGGAAGCTTGTCTTTACAAAATATAATTGATTAAGATATGTCAAGACCTACATTGTCTA AGCACCGGCAAATTAAAATGTCGAGAATCACTTCAGTCAAAAACCTTTATATTCTGTTCTTAATAATGTTTGTGCCAACC TATATCCCATGTAAGGGATCTGGGGAGGAGGCATGTGTCTACAACCATACCTTTTTGCACTATGGGCACTAACCACCCTG AAACTTCCTGCGGTAGCTCCCTCCCTTCAGAGTTACATCATTATCCTGACTCTGTGTAGGTAAATTTTCGTGAAATTTTT GTACAAAAAAAAAAAAAAAA

Claims

Patent claims

1 . Apoptosis-associated nucleic acids comprising: (a) those shown in Table 1 or SEQ ID NO: 1-225

Nucleic acids of clones 1 - 1 24 or the nucleic acids of clones 1 - 124, 1 25, 127, 133, 1 34, 140, 141, 1 44, 145, 146 shown in Table 2, complementary nucleic acids or fragments thereof, (b ) nucleic acids corresponding to the sequences according to (a) in the context of the degeneration of the genetic code and (c) nucleic acids hybridizing with the sequences according to (a) and/or (b) under stringent conditions.

2. Nucleic acids according to claim 1, characterized in that they induce apoptosis after expression in a cell.

3. Nucleic acids according to claim 1 or 2, characterized in that they code for an apoptosis-associated polypeptide.

4. Nucleic acids according to one of claims 1 to 3, characterized in that they come from a eukaryotic organism.

5. Nucleic acids according to claim 4, characterized in that they come from a mammal.

Nucleic acids according to claim 5, characterized in that they are of human origin.

7. Nucleic acids according to claim 1, characterized in that the partial fragments have a length of at least 15 nucleotides.

8. Nucleic acids according to one of claims 1 to 7, characterized in that they are operatively linked to an expression control sequence.

9. Nucleic acids according to claim 8, characterized in that the expression control sequence is a heterologous expression control sequence.

10. Recombinant vector, characterized in that it contains a nucleic acid according to one of claims 1 to 9.

11. Recombinant cell, characterized in that it is transformed or transfected with a nucleic acid according to one of claims 1 to 9 or a vector according to claim 8.

12. Polypeptide, characterized in that it is encoded by a nucleic acid according to claim 1.

3. Pharmaceutical composition comprising a nucleic acid according to one of claims 1 to 9, a vector according to claim 10 or a polypeptide according to claim 1 2, optionally together with pharmaceutically customary carriers and auxiliaries.

1 4. Use of a nucleic acid according to one of claims 1 to 9, a vector according to claim 10, a cell according to claim 1 1 or a polypeptide according to claim 1 2 for producing a diagnostic or therapeutic agent.

1 5. Use according to claim 1 4 for the diagnosis, therapy or prevention of apoptosis-associated diseases.

1 6. Use of a nucleic acid according to one of claims 1 to 9, a vector according to claim 1 0, a cell according to claim 1 1 or a polypeptide according to claim 12 for identifying active substances for the therapy or prevention of apoptosis-associated diseases.

1 7. Use according to claim 1 6, characterized in that the identification takes place in a high-throughput process.

1 8. Use according to claim 1 6 or 1 7, characterized in that the active substances activate or inhibit signaling pathways which are induced by the expression of the nucleic acid.

19. Transgenic non-human animal, (i) which constitutively or inducibly overexpresses the gene of a nucleic acid according to one of claims 1 to 9 or the ANT-1 gene, (ii) which overexpresses the endogenous gene of a nucleic acid according to one of claims 1 to 9 or the ANT-1 gene in inactivated form, (iii) in which the endogenous gene of a nucleic acid according to one of claims 1 to 9 or the ANT-1 gene is completely or partially replaced by a mutated gene of a nucleic acid according to one of claims 1 to 9 or a mutated ANT 1 gene is replaced, (iv) which has a conditional and tissue-specific over- or under-expression of the gene of a nucleic acid according to one of claims 1 to 9 or the ANT-1 gene or (v) which has a conditional and tissue-specific knockout of the gene Nucleic acid according to one of claims 1 to 9 or the ANT-1 gene.

20. Transgenic animal according to claim 19, characterized in that the endogenous promoter of the gene of a nucleic acid according to one of claims 1 to 9 or of the ANT-1 gene has a genetic change which leads to an altered expression of the gene.

21. Transgenic animal according to claim 19 or 20, characterized in that it is a rodent, in particular a mouse.

22. Use of a transgenic animal according to one of claims 19 to 21 for genetic and / or pharmacological examination of apoptotic processes, particularly diseases associated with increased or decreased apoptosis.

23. Cell culture

(i) which constitutively or inducibly overexpresses the gene of a nucleic acid according to one of claims 1 to 9 or the ANT-1 gene, (ii) which overexpresses the endogenous gene of a nucleic acid according to one of claims 1 to 9 or the ANT-1 gene in inactivated form, (iii) in which the endogenous gene of a nucleic acid according to one of claims 1 to 9 or the ANT-1 gene is completely or partially replaced by a mutated gene of a nucleic acid according to one of claims 1 to 9 or a mutated ANT 1 gene is replaced, (iv) which has a conditional and tissue-specific over- or under-expression of the gene of a nucleic acid according to one of claims 1 to 9 or the ANT-1 gene or (v) which has a conditional and tissue-specific knockout of the gene Nucleic acid according to one of claims 1 to 9 or the ANT-1 gene.

24. Cell culture according to claim 23, characterized in that it consists of human cells.

25. Use of a cell culture according to claim 23 or 24 for the genetic and/or pharmacological examination of apoptotic processes, in particular diseases that are associated with increased or decreased apoptosis.