EP1263976A4 - Expression of an antimicrobial peptide via the plastid genome to control phytopathogenic bacteria - Google Patents

Expression of an antimicrobial peptide via the plastid genome to control phytopathogenic bacteria

Info

Publication number
EP1263976A4
EP1263976A4 EP01913116A EP01913116A EP1263976A4 EP 1263976 A4 EP1263976 A4 EP 1263976A4 EP 01913116 A EP01913116 A EP 01913116A EP 01913116 A EP01913116 A EP 01913116A EP 1263976 A4 EP1263976 A4 EP 1263976A4
Authority
EP
European Patent Office
Prior art keywords
expression
antimicrobial peptide
peptide via
plastid genome
phytopathogenic bacteria
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
EP01913116A
Other languages
German (de)
French (fr)
Other versions
EP1263976A1 (en
Inventor
Henry Daniell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Auburn University
University of Central Florida
Original Assignee
Auburn University
University of Central Florida
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Auburn University, University of Central Florida filed Critical Auburn University
Publication of EP1263976A1 publication Critical patent/EP1263976A1/en
Publication of EP1263976A4 publication Critical patent/EP1263976A4/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8281—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for bacterial resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP01913116A 2000-02-29 2001-02-28 Expression of an antimicrobial peptide via the plastid genome to control phytopathogenic bacteria Ceased EP1263976A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US18566200P 2000-02-29 2000-02-29
US185662P 2000-02-29
PCT/US2001/006287 WO2001064927A1 (en) 2000-02-29 2001-02-28 Expression of an antimicrobial peptide via the plastid genome to control phytopathogenic bacteria

Publications (2)

Publication Number Publication Date
EP1263976A1 EP1263976A1 (en) 2002-12-11
EP1263976A4 true EP1263976A4 (en) 2004-12-01

Family

ID=22681933

Family Applications (1)

Application Number Title Priority Date Filing Date
EP01913116A Ceased EP1263976A4 (en) 2000-02-29 2001-02-28 Expression of an antimicrobial peptide via the plastid genome to control phytopathogenic bacteria

Country Status (5)

Country Link
EP (1) EP1263976A4 (en)
AU (1) AU2001241813A1 (en)
CA (1) CA2401957A1 (en)
NZ (1) NZ521660A (en)
WO (1) WO2001064927A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100251425A9 (en) 1998-05-15 2010-09-30 University Of Central Florida Expression of human interferon in transgenic chloroplasts
EP1539965A4 (en) 2002-07-03 2006-02-15 Univ Central Florida Expression of the human igf-1 in transgenic plastids
WO2007053183A2 (en) 2005-05-27 2007-05-10 University Of Central Florida Chloroplasts engineered to express pharmaceutical proteins
CA2682199C (en) 2007-03-30 2016-06-28 University Of Central Florida Research Foundation, Inc. Chloroplasts engineered to express pharmaceutical proteins in edible plants
US10689633B2 (en) 2008-02-29 2020-06-23 The Trustees Of The University Of Pennsylvania Expression of β-mannanase in chloroplasts and its utilization in lignocellulosic woody biomass hydrolysis
WO2011057243A2 (en) 2009-11-09 2011-05-12 University Of Central Florida Research Foundation, Inc. Administration of plant expressed oral tolerance agents
WO2013063049A1 (en) 2011-10-24 2013-05-02 University Of Central Florida Research Foundation, Inc. Orally-administered plastid expressed cholera toxin b subunit-exendin 4 as treatment for type 2 diabetes

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998006860A1 (en) * 1996-08-14 1998-02-19 Novartis Ag Peptide with inhibitory activity towards plant pathogenic fungi
WO1999006564A1 (en) * 1997-07-31 1999-02-11 Sanford Scientific, Inc. Expression of antimicrobial peptide genes in plants, and their use in creating resistance to multiple plant pathogens
WO1999010513A1 (en) * 1997-08-07 1999-03-04 Auburn University Universal chloroplast integration and expression vectors, transformed plants and products thereof

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990011770A1 (en) * 1989-04-11 1990-10-18 Calgene, Inc. Use of animal-derived anti-microbial peptides for control of plant pathogens
US5451513A (en) * 1990-05-01 1995-09-19 The State University of New Jersey Rutgers Method for stably transforming plastids of multicellular plants
US5877402A (en) * 1990-05-01 1999-03-02 Rutgers, The State University Of New Jersey DNA constructs and methods for stably transforming plastids of multicellular plants and expressing recombinant proteins therein

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998006860A1 (en) * 1996-08-14 1998-02-19 Novartis Ag Peptide with inhibitory activity towards plant pathogenic fungi
WO1999006564A1 (en) * 1997-07-31 1999-02-11 Sanford Scientific, Inc. Expression of antimicrobial peptide genes in plants, and their use in creating resistance to multiple plant pathogens
WO1999010513A1 (en) * 1997-08-07 1999-03-04 Auburn University Universal chloroplast integration and expression vectors, transformed plants and products thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DEGRAY GERALD ET AL: "Expression of an antimicrobial peptide via the chloroplast genome to control phytopathogenic bacteria and fungi", PLANT PHYSIOLOGY (ROCKVILLE), vol. 127, no. 3, November 2001 (2001-11-01), pages 852 - 862, XP002299090, ISSN: 0032-0889 *
See also references of WO0164927A1 *

Also Published As

Publication number Publication date
AU2001241813A1 (en) 2001-09-12
WO2001064927B1 (en) 2002-02-07
NZ521660A (en) 2004-05-28
EP1263976A1 (en) 2002-12-11
WO2001064927A1 (en) 2001-09-07
CA2401957A1 (en) 2001-09-07

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