EP1238290A1 - Ultraschalltrichteranordnung - Google Patents

Ultraschalltrichteranordnung

Info

Publication number
EP1238290A1
EP1238290A1 EP00993016A EP00993016A EP1238290A1 EP 1238290 A1 EP1238290 A1 EP 1238290A1 EP 00993016 A EP00993016 A EP 00993016A EP 00993016 A EP00993016 A EP 00993016A EP 1238290 A1 EP1238290 A1 EP 1238290A1
Authority
EP
European Patent Office
Prior art keywords
probe
face
horn
assembly defined
cup member
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP00993016A
Other languages
English (en)
French (fr)
Inventor
Ronald R. Manna
Dan Voic
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Misonix LLC
Original Assignee
Misonix LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Misonix LLC filed Critical Misonix LLC
Publication of EP1238290A1 publication Critical patent/EP1238290A1/de
Withdrawn legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B06—GENERATING OR TRANSMITTING MECHANICAL VIBRATIONS IN GENERAL
    • B06B—METHODS OR APPARATUS FOR GENERATING OR TRANSMITTING MECHANICAL VIBRATIONS OF INFRASONIC, SONIC, OR ULTRASONIC FREQUENCY, e.g. FOR PERFORMING MECHANICAL WORK IN GENERAL
    • B06B3/00—Methods or apparatus specially adapted for transmitting mechanical vibrations of infrasonic, sonic, or ultrasonic frequency

Definitions

  • This invention relates to ultrasonic vibration probes. More particularly,
  • this invention relates to such an ultrasonic probe or horn assembly which is
  • devices of this type include an electronic generator for producing electrical signals with
  • the cavitation bubbles produced by such ultrasonic vibration devices can be utilized to effect changes in the fluid or upon particles suspended therein. Such changes include biological cell disruption, deagglomeration of
  • the cells may be disrupted without the heat or cellular changes
  • the acoustic waves will propagate through the membrane and transfer the cavitation forces to the second liquid
  • Cup Horn One requirement for use of the Cup Horn is that the beaker or test tube diameter be significantly smaller than the distal diameter of the Cup Horn probe
  • liquid is forced to surround the entire probe end in order to provide the transfer fluid for the acoustic wave.
  • FIG 1 shows the relationship of the Cup Horn probe 12, transfer fluid 14 and
  • a cup 16 having a cylindrical sidewall 18, an inwardly
  • sample vessel takes the form of a multi-well microtiter plate or tray.
  • a multi-well microtiter plate or tray is typically
  • the tray is fairly shallow and may contain up to approximately 96 depressions
  • the outer edges of the trays received irregular ultrasonic energy and therefore inconsistent cell breakdown in successive samples.
  • An object of the present invention is to provide an ultrasonic device
  • Another object of the present invention is to provide such an ultrasonic
  • a further object of the present invention is to provide such an ultrasonic device which does not heat the fluid or the sample liquids, and which require minimum energy to operate, thereby allowing the use of the device on existing
  • the present invention is directed to an ultrasonic sonication device which
  • magnetostnctive type resonates in sympathy with the transducer and either
  • An ultrasonic horn assembly comprises, in accordance with the present
  • an ultrasonic horn or probe having an axis and a distal end with an
  • probe is disposed at least approximately at an antinode of ultrasonic vibration of
  • a cup member is attached to the horn or probe at least
  • the reservoir as a shallow reservoir covering essentially only the end face of the probe.
  • a small or marginal circumferential surface of the probe, contiguous with the end face thereof, may be submerged in the coupling liquid, as well.
  • the load placed upon the probe is decreased owing to the reduction in the area
  • the cup member is attached to the horn or probe via a flexible coupling element such as an O-ring or an annular elastomeric membrane.
  • a flexible coupling element such as an O-ring or an annular elastomeric membrane.
  • the lower wall is provided with at least one port for feeding liquid to the reservoir.
  • the port is one of at least a pair of ports
  • the end face of the probe is disposed in a first plane and an upper
  • This spacing optimizes the acoustic effects of the ultrasonic energy on specimens contained
  • the probe end face is provided with a plurality of grooves for receiving peripheral lower edges of the
  • the end face of the probe is circular, the end face has a diameter
  • the probe is
  • an annular concavity for providing or enhancing uniformity of the ultrasonic wave field
  • Figure 1 is a cross-sectional view, taken along an axial plane, of an ultrasonic sonication device in accordance with the prior art.
  • Figure 2 is a cross-sectional view, taken along an axial plane, of an ultrasonic sonication device in accordance with the present invention.
  • Figure 3 is a cross-sectional view, taken along an axial plane, of another ultrasonic sonication device in accordance with the present invention.
  • Figure 4 is a top plan view of the ultrasonic sonication device of Figure 2
  • Figure 5 is a partial cross-sectional view taken along line V-V in Figure 4.
  • Figure 6 is a detail, on a larger scale, of a portion VI of Figure 5.
  • Figure 7 is an enlarged top plan view similar to Figure 4, showing flow
  • an ultrasonic sonication device comprises a
  • horn or probe 32 having an axis 34 defining a direction of ultrasonic standing
  • Probe 32 has a distal end portion 36 formed with an active end face 38 oriented transversely to axis 34 and provided with at least one pair
  • Distal end portion 36 of probe 32 is further formed with an annular groove 44 receiving an elastomeric O-ring seal 46.
  • the ultrasonic sonication device of Figure 2 additionally comprises a cup member 48 having a vertical cylindrical sidewall 50 and a horizontal annular flange 52 extending inwardly from a lower end of the sidewall.
  • An inner cup member 48 having a vertical cylindrical sidewall 50 and a horizontal annular flange 52 extending inwardly from a lower end of the sidewall.
  • a modified ultrasonic sonication device comprises a cup member 60 having a sidewall 50' with a larger diameter than sidewall 50 of cup member 48.
  • An inner periphery of an annular flange 52' is spaced from and connected to the outer periphery of distal horn portion 36 by
  • Membrane 62 is sealingly fixed along an
  • Figures 4, 5, and 6 depict the use of the sonication device of Figure 2 with a microtiter tray or plate 64 having a plurality of specimen-receiving wells
  • tray 64 or cells 66 disposed in a rectangular array.
  • Four corners 68 of tray 64 rest on
  • Tray 64 is conventionally configured to have a peripheral lower rim 72
  • Probe 32 functions in part as a velocity transformer which amplifies the
  • Probe 32 can be designed and constructed using standard techniques known to the art. However, several important operating characteristics must be used.
  • distal end face 38 of probe 32 must be large enough to cover the entire area of bottom surface 70 of
  • microtiter tray 64 In the embodiment described herein, distal end face 38 is
  • microtiter tray wells 66 must not be less than 0.125 inches from an outer edge 74 of probe end face 38. If a tray cell 66 is located at edge 74 or within 0.125
  • Second is that it is advantageous if a uniform amplitude of vibration is generated across the entire end face 38 of probe 32. If significantly non-uniform vibrations are present, then non-uniformity of processing in the microtiter wells 66 will result. In order to obtain this uniform
  • Grooves or reliefs 40, 42, etc. are machined or otherwise formed in probe end face 38 ( Figure 6) to allow microtiter tray edge or rim 72 to sit in these recesses. In this way, the bottom surface 70 of microtiter tray 64 sits within 0.100 inches (preferably between about 0.001 and 0.100 inches) of the
  • probe 32 is particularly shown in Figures 4-6. Of course, probe 32
  • connection to the transducer can be accomplished by a threaded stud (not shown) or other techniques well known to the art.
  • the seal provided by O-ring 46 or membrane 62 is elastomeric to
  • cup member 48 or 60 provide a compliant joint between cup member 48 or 60 and probe 32. This seal is liquid tight and yet isolates cup member 48 or 60 from the vibrations transmitted by probe 32. This isolation prevents loading and possible detuning
  • Cup members 48 and 60 are fabricated alternatively from clear acrylic and clear polyvinylchloride. However, other materials such as thermoplastics,
  • metals, ceramics or thermosets may be used with equal results.
  • cup member 48 and 60 Several features of cup member 48 and 60 are important to the operation of the device. First, cup members 48 and 60 must have an internal diameter just slightly greater than the diagonal dimension of the microtiter tray 64. This centers the tray 64 with respect to the end face 38 of probe 32, as
  • Microtiter tray 64 sits on cup surface 76 and does not contact probe 32 at any point. If tray 64 is allowed
  • cup member 48 or 60 must incorporate liquid fittings or ports 54 and 56, to allow coupling fluid 58 to be pumped in and out of the cup member.
  • FIG. 7 shows general paths 78 of fluid flow under microtiter tray 64 from one port or fitting 54 to the other port 56.
  • the coupling fluid 58 has maximum cooling effect and reduces or eliminates splashing onto the top of the tray 64, thereby preventing contamination of the
  • port elements 54, 56 are standard
  • the coupling fluid or liquid can be plain tap water, saline, distilled water or, if
  • glycol and water may be employed.
  • a thin plastic film (not shown) should be applied to the top
  • microtiter tray 64 in a fashion known to the art. This thin film prevents loss of
  • the film is deemed essential in use of the ultrasonic sonication
  • Cup member 48, 60 must incorporate features such as a counterbore to prevent slippage of the cup relative to probe 32. This prevents the cup from lowering with respect to the probe end face 38 and maintains the clearance between the bottom surface 70 of microtiter tray 64 and the probe end face.

Landscapes

  • Engineering & Computer Science (AREA)
  • Mechanical Engineering (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Transducers For Ultrasonic Waves (AREA)
  • Apparatuses For Generation Of Mechanical Vibrations (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
EP00993016A 1999-12-10 2000-12-01 Ultraschalltrichteranordnung Withdrawn EP1238290A1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US17014199P 1999-12-10 1999-12-10
US170141P 1999-12-10
PCT/US2000/042517 WO2001046714A1 (en) 1999-12-10 2000-12-01 Ultrasonic horn assembly

Publications (1)

Publication Number Publication Date
EP1238290A1 true EP1238290A1 (de) 2002-09-11

Family

ID=22618712

Family Applications (1)

Application Number Title Priority Date Filing Date
EP00993016A Withdrawn EP1238290A1 (de) 1999-12-10 2000-12-01 Ultraschalltrichteranordnung

Country Status (4)

Country Link
EP (1) EP1238290A1 (de)
JP (1) JP2003517848A (de)
CA (1) CA2393607A1 (de)
WO (1) WO2001046714A1 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT1712920E (pt) * 2000-07-07 2009-04-09 Merck Serono Sa Diagnóstico precoce de doenças conformacionais
DE10148916A1 (de) * 2001-10-04 2003-04-17 Beatrix Christa Meier Ultraschallvorrichtung
US7611840B2 (en) 2004-08-03 2009-11-03 Agency For Science, Technology And Research Method and device for the treatment of biological samples
FR2948585A1 (fr) * 2009-07-31 2011-02-04 Inst Pierre Vernier Sonotrode a gorge et machine a usinage ultrasonore integrant la sonotrode
EP2489427A1 (de) 2011-02-16 2012-08-22 Helmholtz-Zentrum für Infektionsforschung GmbH Vorrichtung und Verfahren zur Herstellung und Analyse von Prionen
US20230264116A1 (en) * 2022-02-22 2023-08-24 Sonics & Materials, Inc. Flow Cell Horn And Method Of Tuning

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3937236A (en) * 1974-10-07 1976-02-10 Mdt Chemical Company Ultrasonic cleaning device
US4930532A (en) * 1989-02-17 1990-06-05 Ipco Corporation Beaker holder for use with ultrasonic cleaning device
US5185728A (en) * 1990-10-31 1993-02-09 Cyber Scientific Omnidirectional ultrasonic transducer
US5459699A (en) * 1992-08-25 1995-10-17 Industrial Sound Technologies Method and apparatus for generating high energy acoustic pulses
US6071480A (en) * 1994-12-22 2000-06-06 Abbott Laboratories Method for generating a standing sonic wave, methods of sonication with a standing sonic wave, and a standing sonic wave sonicator

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO0146714A1 *

Also Published As

Publication number Publication date
JP2003517848A (ja) 2003-06-03
WO2001046714A1 (en) 2001-06-28
CA2393607A1 (en) 2001-06-28

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