EP1220606A4 - Methods of producing a target molecule in a transgenic animal and purification of the target molecule - Google Patents

Methods of producing a target molecule in a transgenic animal and purification of the target molecule

Info

Publication number
EP1220606A4
EP1220606A4 EP00970945A EP00970945A EP1220606A4 EP 1220606 A4 EP1220606 A4 EP 1220606A4 EP 00970945 A EP00970945 A EP 00970945A EP 00970945 A EP00970945 A EP 00970945A EP 1220606 A4 EP1220606 A4 EP 1220606A4
Authority
EP
European Patent Office
Prior art keywords
target molecule
methods
purification
producing
transgenic animal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP00970945A
Other languages
German (de)
French (fr)
Other versions
EP1220606A1 (en
Inventor
Harry Meade
Scott P Fulton
Yann Echelard
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
rEVO Biologics Inc
Original Assignee
GTC Biotherapeutics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GTC Biotherapeutics Inc filed Critical GTC Biotherapeutics Inc
Publication of EP1220606A1 publication Critical patent/EP1220606A1/en
Publication of EP1220606A4 publication Critical patent/EP1220606A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/15—Humanized animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The invention provides systems and methods for the production and purification of target molecules present in biological systems. The systems and methods according to the invention utilize transgenic expression of multivalent binding polypeptides, as affinity media, to purify such target molecules. The transgenic multivalent binding polypeptides bind both the target molecules, e.g., a bindable epitope of a target molecule, and a matrix.
EP00970945A 1999-10-14 2000-10-16 Methods of producing a target molecule in a transgenic animal and purification of the target molecule Withdrawn EP1220606A4 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US15974899P 1999-10-14 1999-10-14
US159748P 1999-10-14
US20466200P 2000-05-17 2000-05-17
US204662P 2000-05-17
PCT/US2000/028589 WO2001026455A1 (en) 1999-10-14 2000-10-16 Methods of producing a target molecule in a transgenic animal and purification of the target molecule

Publications (2)

Publication Number Publication Date
EP1220606A1 EP1220606A1 (en) 2002-07-10
EP1220606A4 true EP1220606A4 (en) 2004-04-14

Family

ID=26856252

Family Applications (1)

Application Number Title Priority Date Filing Date
EP00970945A Withdrawn EP1220606A4 (en) 1999-10-14 2000-10-16 Methods of producing a target molecule in a transgenic animal and purification of the target molecule

Country Status (14)

Country Link
EP (1) EP1220606A4 (en)
JP (1) JP2003511048A (en)
KR (1) KR100772465B1 (en)
CN (1) CN1322003C (en)
AU (1) AU781944B2 (en)
BR (1) BR0014860A (en)
CA (1) CA2382741A1 (en)
HU (1) HUP0203537A2 (en)
IL (1) IL149003A0 (en)
MX (1) MXPA02003702A (en)
NO (1) NO20021686L (en)
NZ (1) NZ518263A (en)
RU (1) RU2002112460A (en)
WO (1) WO2001026455A1 (en)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030049694A1 (en) * 2001-09-10 2003-03-13 Chung-Hsiun Wu Production of fusion proteins and use for identifying binding molecules
US6800462B2 (en) * 2001-09-10 2004-10-05 Abgenomics Corporation Production of recombinant proteins in vivo and use for generating antibodies
CN1283785C (en) * 2002-05-20 2006-11-08 上海杰隆生物工程股份有限公司 Method of producing human forcing erythrogenin using transgene animal mammary gland
CA2612479A1 (en) * 2005-06-29 2007-05-10 Cameron Malcolm Lang Clokie Production of bone morphogenic proteins (bmps) in transgenic mammals
CN101802210A (en) * 2007-06-13 2010-08-11 斯特林贝尔德生物技术北美公司 In milk, produce the transgene mammal of foreign protein
WO2010036976A2 (en) 2008-09-25 2010-04-01 Transgenrx, Inc. Novel vectors for production of antibodies
WO2010036978A2 (en) 2008-09-25 2010-04-01 Transgenrx, Inc. Novel vectors for production of growth hormone
EP2417263B1 (en) 2009-04-09 2015-09-23 ProteoVec Holding L.L.C. Production of proteins using transposon-based vectors
CN104302312A (en) * 2011-12-19 2015-01-21 Lfb美国股份有限公司 Recombinant human alpha-1-antitrypsin for the treatment of inflammatory disorders
EP2956480B1 (en) 2013-02-13 2019-09-04 Laboratoire Français du Fractionnement et des Biotechnologies Highly galactosylated anti-tnf-alpha antibodies and uses thereof
EP2956003A2 (en) 2013-02-13 2015-12-23 Laboratoire Français du Fractionnement et des Biotechnologies Proteins with modified glycosylation and methods of production thereof
CN105358228A (en) 2013-07-05 2016-02-24 法国血液分割暨生化制品实验室 Affinity chromatography matrix
FR3025515B1 (en) 2014-09-05 2016-09-09 Lab Francais Du Fractionnement PROCESS FOR PURIFYING MONOCLONAL ANTIBODY
FR3034420A1 (en) 2015-03-31 2016-10-07 Lab Francais Du Fractionnement ANTI-CD303 MONOCLONAL ANTIBODIES
JP6712125B2 (en) * 2015-09-04 2020-06-17 株式会社フジクラ Connection structure of oxide superconducting wire and method for manufacturing the same
FR3060395B1 (en) 2016-12-16 2019-05-24 Laboratoire Francais Du Fractionnement Et Des Biotechnologies COMBINATION OF ANTI-CD303 AND ANTI-HER2 ANTIBODIES
FR3060394B1 (en) 2016-12-16 2019-05-24 Laboratoire Francais Du Fractionnement Et Des Biotechnologies COMBINATION OF ANTI-CD303 AND ANTI-AMHRII ANTIBODIES
FR3076294B1 (en) 2017-12-29 2022-01-28 Lab Francais Du Fractionnement METHOD FOR PURIFYING ANTIBODIES FROM RAW MILK
WO2023118241A1 (en) 2021-12-20 2023-06-29 Vetoquinol Sa Anti-canine interleukine-31-receptor a (il-31ra) antibodies and the uses thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991007428A1 (en) * 1989-11-20 1991-05-30 Novo Nordisk A/S A protein purification method

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5639940A (en) * 1994-03-03 1997-06-17 Pharmaceutical Proteins Ltd. Production of fibrinogen in transgenic animals
CN1114357A (en) * 1994-07-01 1996-01-03 唐勇炜 Process for producing recombinative polypeptide using transgene animal

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991007428A1 (en) * 1989-11-20 1991-05-30 Novo Nordisk A/S A protein purification method

Also Published As

Publication number Publication date
CA2382741A1 (en) 2001-04-19
MXPA02003702A (en) 2002-09-18
KR20030022759A (en) 2003-03-17
CN1322003C (en) 2007-06-20
NO20021686L (en) 2002-06-12
NZ518263A (en) 2004-03-26
CN1387399A (en) 2002-12-25
IL149003A0 (en) 2002-11-10
AU781944B2 (en) 2005-06-23
AU8025400A (en) 2001-04-23
HUP0203537A2 (en) 2003-02-28
RU2002112460A (en) 2004-02-27
EP1220606A1 (en) 2002-07-10
JP2003511048A (en) 2003-03-25
BR0014860A (en) 2003-07-08
NO20021686D0 (en) 2002-04-10
WO2001026455A1 (en) 2001-04-19
KR100772465B1 (en) 2007-11-02

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