EP1217893A1 - Methode permettant de renforcer l'immunite de vegetaux et d'animaux - Google Patents

Methode permettant de renforcer l'immunite de vegetaux et d'animaux

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Publication number
EP1217893A1
EP1217893A1 EP99945792A EP99945792A EP1217893A1 EP 1217893 A1 EP1217893 A1 EP 1217893A1 EP 99945792 A EP99945792 A EP 99945792A EP 99945792 A EP99945792 A EP 99945792A EP 1217893 A1 EP1217893 A1 EP 1217893A1
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EP
European Patent Office
Prior art keywords
solution
vitamin
plants
salicylic acid
mineral
Prior art date
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Application number
EP99945792A
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German (de)
English (en)
Inventor
Steve Wood
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Individual
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Individual
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Publication date
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Publication of EP1217893A1 publication Critical patent/EP1217893A1/fr
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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
    • A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
    • A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
    • A01N37/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/06—Aluminium; Calcium; Magnesium; Compounds thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/03—Algae

Definitions

  • the present invention relates to a method for improving the immunity of plants and animals .
  • Various methods are known for improving the vigour of seedlings. For example, it is known to pre-soak seeds in a solution of colchicine . It is thought that such treatment increases the number of chromosome sets in cells of the seeds, thus inducing a change known as polyploidy. It is also known to improve the growth of seedlings with growth hormones, e.g. cytokinin. Furthermore, it is known to trigger a plants' defences against disease by adding salicylic acid, as reported in Science Volume 237, p.1601, Science Volume 250, p.1002 and 1004. Notwithstanding these techniques, however, it is believed that no-one has successfully developed a method for improving the immunity of plants from one generation to another.
  • the present invention is also directed towards assisting the body to fight off disease .
  • the present invention is directed to compositions and methods for improving immunity in plants and animals.
  • compositions for treating plants or animals comprising an aqueous solution of i) a mixture of mineral-based nutrients, ii) a mixture of B vitamins, iii) acetyl salicylic acid or a salt thereof, and iv) at least one plant growth inducing hormone or hormone-like compound.
  • the plant growth inducing hormone or hormone-like compound is selected from the group consisting of cytokinins, auxins, gibberellins, naphthalenic acid, naphthalene acetic acid, indole 3 butyric acid, indole 3 acetic acid and salts thereof, and mixtures thereof .
  • the plant growth hormone is a cytokinin, preferably a cytokinin derived from ascophyllum nodosum.
  • the plant growth hormone is present in the form of kelp.
  • the plant growth inducing hormone or hormone-like compound is selected from the group consisting of naphthalene acetic acid, indole 3 butyric acid, water soluble salts thereof, and mixtures thereof .
  • the mineral-based nutrients are selected from the group consisting of nitrates, nitric acid, phosphates, citrates, and sulphates, and mixtures thereof.
  • the mineral-based nutrients are selected from the group consisting of nitrates, phosphates, citrates and sulphates of alkali metals, ammonia, alkaline earth metals and magnesium, and mixtures thereof .
  • the mineral-based nutrients are selected from the group consisting of calcium nitrate, potassium sulphate, potassium nitrate, monopotassium phosphate, magnesium sulphate and ammonium nitrate, and mixtures thereof.
  • the mineral-based nutrients are supplemented with at least one supplementary ingredient selected from the group consisting of minerals, vitamins and plant extracts.
  • the minerals are selected from the group consisting of minerals of zinc, manganese, iron, chromium, cobalt, copper, vanadium, molybdenum, selenium, fluorine, chlorine and iodine, and mixtures thereof.
  • the plant extract is selected from the group consisting of yucca extract, wheat grass juice, barley juice, gingko biloba, pine bark extract and milk thistle, and mixtures thereof.
  • the vitamins are selected from the group consisting of vitamin C, vitamin D, vitamin E, vitamin K and ⁇ -carotene, and mixtures thereof .
  • the B vitamins are selected from the group consisting of vitamin Bl
  • vitamin B2 riboflavin
  • vitamin B6 pyridoxine
  • vitamin B3 niacin and/or niacinamide
  • vitamin B5 pantothenic acid
  • vitamin B12 cobalamin and/or cyanocobalamin
  • B complex and mixtures thereof B complex and mixtures thereof .
  • the composition may contain spirulina, chlorella, alfalfa, vegetable enzymes, dom quai, grape seed extract, ginseng, hemp oil, safflower oil carrot powder, flax seed, bilberry, lecithin, celanine, silymarin, royal jelly, co-enzyme Q10, pectin, bran, cinnamon and vegetable enzymes, and mixtures thereof.
  • the mixture of mineral-based nutrients is in a concentration of less than 1000 g per 100 litres, especially less than 500 g per 100 litres of solution
  • the mixture of B vitamins is in a concentration of less than 1200 mg per 100 litres of solution
  • the acetyl salicylic acid or a salt thereof is in a concentration of less than 1 g of solution
  • the plant growth inducing hormone or hormone-like compound is in a concentration of less than 50 g, especially less than 5 g per 100 litres of solution.
  • Another aspect of the present invention provides a method for treating plants comprising feeding the plants or seedlings thereof with an aqueous solution of i) a mixture of mineral-based nutrients, ii) a mixture of B vitamins, iii) acetyl salicylic acid or a salt thereof, and iv) at least one plant growth inducing hormone or hormone-1ike compound.
  • the mineral-based nutrients are selected for root development. In another embodiment, the mineral-based nutrients are selected for growth. In yet another embodiment, the mineral-based nutrients are selected for flower development.
  • the mineral-based nutrients are selected for seed or fruit development.
  • the solution of the present invention has the components in low concentrations in water. Preferably, the total concentration of ingredients is less than about
  • the plant growth inducing hormone or hormone-like compound is selected from the group consisting of naphthalene acetic acid, indole 3 butyric acid, water soluble salts thereof, and mixtures thereof .
  • the pH of the solution is from 5.5 to 8.0, especially from 6.5 to 7.5.
  • the plant is fed a solution comprising up to about 500 grams of each nutrient compound, up to about 1000 milligrams of each B vitamin, up to about 10 grams of the plant growth hormone or plant growth hormone-like compound, and up to about 2000 milligrams of acetyl salicylic acid or a salt thereof per 200 litres of solution.
  • the present invention also provides a method of treating plants comprising feeding the plants with a composition of the present invention by at least one of the procedures selected from the group consisting of root feeding and spraying leaves of the plants .
  • the plant is selected from the group consisting of leaf vegetables, fruiting vegetables, flowering plants, and seeding plants .
  • the plant is selected from cucumbers, lettuce, tomatoes, peppers, cabbage, corn, grass, flax and hemp.
  • composition is fed to the plants hydroponically.
  • Another aspect of the present invention provides a method for improving the immunity in plants, comprising the steps of:
  • a first portion of acetyl salicylic acid or a salt thereof is added to the solution during a first time period of application of the solution, and a second portion of acetyl salicylic acid or a salt thereof is added to the solution during a second time period of application of the solution, the second time period being separated from the first time period by a third time period, all of the acetyl salicylic acid or salt thereof being administered before the plants flower.
  • the seedlings are from seeds which have been pre-soaked in colchicine solution.
  • the method comprises : (a) soaking seeds of a plant in a 0.05 to 0.15 wt./wt.% solution of colchicine in water and allowing the seeds to sprout into seedlings;
  • the nutrients are selected from the group consisting of calcium nitrate, potassium sulphate, potassium nitrate, monopotassium phosphate, magnesium sulphate, ammonium nitrate, nitric acid, trace elements .
  • the first solution contains ingredients selected from the group consisting of carrot powder, flax seed, bilberry, lecithin, milk thistle, celanine, silymarin, royal jelly, gingko biloba, co- enzyme Q10, pine bark extract, PYCNOGENOL (trade mark) French maritime pine bark extract, pectin, bran, cinnamon and vegetable enzymes .
  • the plant growth hormone is selected from cytokinins, auxins, gibberellins and mixtures thereof.
  • the plant growth hormone is a cytokinin, preferably a cytokinin derived from ascophyllum nodosum.
  • the plant growth hormone or plant growth hormone-like compound is selected from the group consisting of auxins, gibberellins and naphthalenic acid, naphthalene acetic acid, indole 3 butyric acid, indole 3 acetic acid and water soluble salts thereof, and mixtures thereof.
  • the solution of nutrients, at least one plant growth hormone or plant growth hormone-like compound, and a mixture of B vitamins is administered substantially continuously to the seedlings and plants . The method is preferably carried out hydroponically.
  • a solution for administration to animals in the form of a drink or an injectable solution, which comprises an aqueous solution of i) a mixture of mineral-based nutrients, ii) a mixture of B vitamins, iii) acetyl salicylic acid or a salt thereof, and iv) at least one plant growth inducing hormone or hormone-like compound.
  • the solution may contain ingredients for the treatment or alleviation of certain animal diseases or conditions or be taken with ingredients for the treatment or alleviation of certain diseases or conditions.
  • ingredients include dom quai, gingko biloba, medical marijuana, serotonin, L-dopamine, psyllum, red ginseng.
  • At least one ingredient selected from whey, creatine, glutamine may be added to the solution, in drink form.
  • the present invention also provides a method of treating animals comprising feeding the animal, in drink form, the solution of the present invention.
  • the invention provides a method for treating animals comprising administrating by injection the solution of the present invention.
  • the animal is a human.
  • the human is fed from 1 to 6 fluid ounces of the solution daily, wherein the solution has up to about 500 grams of each nutrient compound, up to about 1000 milligrams of each B vitamin, up to about 10 grams of the plant growth hormone or plant growth hormone-like compound, and up to about 2000 milligrams of acetyl salicylic acid or a salt thereof per 200 litres of solution.
  • the solution of the present invention improves the immunity of plants .
  • it important to feed the plants with a solution which comprises nutrients, a plant growth hormone or hormone-like compound, a mixture of B vitamins, and acetyl salicylic acid or a salt thereof.
  • the invention is first discussed in relation to root feeding.
  • acetyl salicylic acid it will be understood that salts thereof could be used instead.
  • root feeding seedlings although the acetyl salicylic acid may be added continuously, it is preferable that, after the initial feeding with acetyl salicylic acid, there be a period of time when feeding of the seedlings with the acetyl salicylic acid is discontinued. Disease-inducing pests may be introduced to "inoculate" the plants.
  • the seeds first be soaked in a solution of colchicine until the seed just start to sprout.
  • the colchicine increases the vigour and symmetry of the resulting plant .
  • a suitable concentration of colchicine in water is from about 0.05 to about 0.15 wt . /wt . % .
  • the sprouting seeds are preferably put into a rooting medium.
  • a rooting medium which is useful and commonly used, especially in hydroponic methods, is known as rockwool .
  • Each sprouting seed is placed on a small rockwool cube, eg. 2.5cm x 2.5cm.
  • a plurality of such rockwool cubes are placed on a table which permits water or nutrient solutions to feed the seedlings.
  • a preferred table is set up to provide an ebb and flow hydroponic system. It is believed that an ebb and flow system promotes better growth than a continuous feeding system.
  • the seeds on the rockwool cubes the sprouting seeds are placed on the table, the seeds are then root fed until the plants which grow from the seeds reach maturity. The duration depends on the type of seeds being grown.
  • the growing seedlings and plants are root fed with a solution of nutrients, at least one plant growth hormone or plant growth hormonelike compound, a mixture of B Vitamins, and acetyl salicylic acid or its salts.
  • acetyl salicylic acid is added to the solution, wither for the whole period or time, or preferably in spaced apart segments of time.
  • the nutrients are selected from those commonly used to fertilize plants. Generally the nutrients are selected from ammonium nitrate, potassium nitrate, calcium nitrate, potassium sulphate, magnesium sulphate, and monopotassium phosphate. There may also be nitric acid and/or trace elements . The ratios and concentrations of each nutrient in the nutrient solution will depend on the type of plant that is being fed, as will be understood by those skilled in the art. The nutrient solution is selected to initially provide optimum conditions for rooting and then for blooming. After blooming, the nutrient solution may be changed to one which is more conducive to providing optimum conditions for fruit and seed production. If the plants are being grown without regard to blooming, i.e.
  • concentrations of ammonium nitrate may be in the range of 0 to 0.006wt . /wt . %, potassium nitrate in the range of 0 to 0.1 wt./wt%, potassium sulphate in the range of 0 to 0.03 wt./wt.%, magnesium sulphate in the range of 0 to 0.05 wt . /wt . % and monopotassium phosphate in the range of 0 to 0.03 wt./wt%.
  • Other ingredients may be added to control pH, and trace elements may also be present .
  • the growth hormone may be a cytokinin, auxin or gibberellins, naphthalenic acid, naphthalene acetic acid, indole 3 butyric acid, indole 3 acetic acid, water soluble salts thereof, and mixtures thereof. Cytokinins are preferable .
  • a suitable cytokinin may be extracted from ascophyllum nodosum. Suitable sources of ascophyllum nodosum are MICRO MIST 300 (trade mark) , available from Kelp Products International, Concord, Ontario and NITROZYME (trade mark) .
  • the growth hormone is preferably present in a concentration of from 0.5 to 5 grams per 10 litres (10 to 100 grams per 50 U.S. gallons) of solution.
  • ⁇ -naphthalenic acid ⁇ -naphthalene acetic ac ' id, sometimes known as INAA or NAA
  • indole 3 butyric acid sometimes known as IBA
  • water soluble salts thereof and mixtures thereof.
  • the root feeding solution also contains a mixture of B vitamins .
  • the B vitamins are selected from Bl (thiamin) , B2 (riboflavin) , vitamin B3 (niacin, niacinamide) , B5 (pantothenic acid) , B6 (pyridoxine) , B complex and B12 (cobalamin, cyanocobalamin) .
  • Typical quantities useful in the present invention are 7 to 22 ml Vitamin Bl, 100 to 300 mg Vitamin B2 , 250 to 750 mg Vitamin B5 , 250 to 750 mg Vitamin B6 and 1000 to 3000 mg Vitamin B12 per 200 litres of solution.
  • the B Vitamins are also believed to be useful for maintaining the pH balance. Omission of the B Vitamins from the root-feeding solution tends to cause the pH to go too high, with resulting stunting of growth.
  • Minerals may also be added, e.g. iron, and trace quantities of other minerals, e.g. chromium, copper, iodine, manganese, sulphur, zinc, boron.
  • other minerals e.g. chromium, copper, iodine, manganese, sulphur, zinc, boron.
  • ingredients of the root-feeding solution may be selected from chlorella, spirulina, wheat grass juice, barley juice, yucca extract, barley juice, alfalfa, carrot powder, flax seed, bilberry, lecithin, milk thistle, royal jelly, gingko biloba, co-enzyme Q10, pine bark extract, PYCNOGENOL (trade mark) pine bark extract, pectin, bran, cinnamon and vegetable enzymes.
  • GREEN STUFF. (trade mark) supplement available from New Sun Canada.
  • salicylic acid or its salts e.g. acetyl salicylic acid.
  • acetyl salicylic acid may be applied during the second week of growth of the seedling.
  • An effective concentration is about 0.5 to 4 grams acetyl salicylic acid per 200 litres of solution.
  • the first introduction is believed to trigger the immune system of the plant .
  • a second application of acetyl salicylic acid may be made in the fifth or sixth week of growth.
  • a higher concentration of acetyl salicylic acid is used, e.g. 1 to 9 grams per 200 litres of solution.
  • the acetyl salicylic acid preferably is not administered constantly, but at intervals as described above, for best results. Too much acetyl salicylic acid leads to mutation of the plant.
  • disease- inducing pests are introduced early in the growth cycle. Although they can be introduced prior to the first application of acetyl salicylic acid, it is best to introduce them during or after the first application of acetyl salicylic acid.
  • the pests must be introduced before buds start to flower. Pests such as mealy bugs, white flies, moulds, spider mites and other predators may be introduced to the plants, depending on the immunity trait that is wished to be induced in the plant.
  • the pests become a nuisance, e.g. in a greenhouses, then they can be removed using natural predators such as bees, hover flies, tachinid flies, green lace wings, praying mantis, ladybugs (ladybirds), ground beetles.
  • natural predators such as bees, hover flies, tachinid flies, green lace wings, praying mantis, ladybugs (ladybirds), ground beetles.
  • pyrethrum was used in an attempt to remove pest insects and a large proportion of the seedlings died.
  • the seeds are collected for planting future generations . Such seeds have been found to be resistant to the diseases which had been introduced in the process of the present invention.
  • the present method be applied in hydroponic growing situations for best results.
  • the method can be used in field situations, especially where there is an irrigation system which is adapted to feed the seedlings and plants relatively continuously .
  • Test have been conducted in field situations without treating the seeds with colchicine but with regularly feeding the plants with a solution of the present invention and without deliberate introduction of pests. In such situations, the plants have been more vigorous than similar plants which have not been fed with solution of the present invention.
  • the plants When leaf feeding plants, the plants may be sprayed with a solution of the present invention, at intervals, e.g. daily.
  • a patch of hemp plants were grown using a solution of the present invention and compared with a control patch of hemp plants which were watered only, relying on soil ' nutrients only to supply the nutrients for growth.
  • the hemp plants which were sprayed with a solution of the present invention were more vigorous, grew faster and taller, less prone to disease, were greener and generally more healthy than the control plants.
  • the present invention provides disease-resistant seeds . Clearly they may be used in any situation but in locations where plants are grown hydroponically, infestations with spider mites, moulds and other pests have been a particular problem.
  • the present invention should reduce the need for crop insurance because of the greater immunity and desirability of the plants.
  • the solution of the present invention has utility in the treatment of seedlings and plants.
  • the inventor believes that there is a corollary with respect to animals in that damaged or cancerous cells are akin to the introduction of the disease-inducing pests in plants and that improved immunity can be obtained by adding suitable nutrients, plant growth hormones and acetylsalicylic acid.
  • aqueous solutions of the present invention may be administered as a drink or administered intravenously or by injection.
  • a substantially neutral pH e.g. between about 6.5 and 7.5.
  • fruit juice or blended fruit may be added.
  • various fruits e.g. bananas, grapes, orange, apple
  • the consistency may be like yoghurt or a fruit juice.
  • Other compounds may be added according to the intended effect or the disease being treated. For example gingko biloba, ginseng, dom quai and other natural herbal remedies may be added.
  • whey and/or creatine and/or glutamine may be added.
  • oestrogen may be added. It is believed that testosterone is beneficial as part of the solution for males, and oestrogen and/or progesterone for females.
  • other substances such as mood enhancing substances are added to improve the effect. Examples of such other substances include serotonin, norepinephrine, dopamine, norethin, vasopressin and oxytosin.
  • the mineral-based nutrients are selected from those commonly used to protect and enhance the animal metabolism.
  • Typical minerals include calcium, potassium, magnesium, zinc, manganese, selenium, boron, chromium, molybdenum, vanadium, iron, copper, phosphorus, iodine, as will be understood by those skilled in the art.
  • suitable mineral-based nutrients are calcium nitrate, calcium citrate, potassium sulphate, potassium nitrate, potassium citrate, monopotassium phosphate, magnesium sulphate, zinc citrate, manganese citrate, potassium iodide, chelated calcium, magnesium, iron, chromium, molybdenum and selenium.
  • the preferred mineral-based nutrients are potassium citrate, monopotassium phosphate, calcium citrate and chelated magnesium.
  • the plant growth hormone may be a cytokinin, auxin or gibberellin. Cytokinins are preferable. A suitable cytokinin may be extracted from ascophyllum nodosum. Suitable sources of ascophyllum nodosum are MICRO MIST 300 (trade mark) , available from Kelp Products International, Concord, Ontario and NITROZYME (trade mark) . It is expected that the plant growth hormone preferably will be present in a concentration of from about 10 to 100 grams per 200 litres. Other plant growth hormones or hormone-like substances are also beneficial, e.g. ⁇ -naphthalenic acid, sometimes known as INAA, and indole 3 butyric acid, sometimes known as IBA. One source of ⁇ -naphthalenic acid is LIQUINOX START
  • LIQUINOX START also contains vitamin B-l, chelated iron, phosphoric acid and a yucca extract base.
  • the solution also contains a mixture of B-vitamins.
  • the B-vitamins are selected from Bl (thiamin) , B2 (riboflavin) , B6 (pyridoxine) , B3 (niacin, niacinamide) , B5 (pantothenic acid) , B complex and B12 (cobalamin, cyanocobalamin) and mixtures thereof. It is expected that typical quantities useful in the present invention will be 7 to 22 ml of vitamin Bl, 100 to 300 mg Vitamin B2 , 250 to 750 mg vitamin B5, 250 to
  • Vitamin B6 750 mg Vitamin B6 and 1000 to 3000 meg Vitamin B12 per 200 litres.
  • the B Vitamins are also useful for maintaining the pH balance.
  • ingredients of the- solution may be selected from chlorella, spirulina, wheat grass juice, yucca extract, barley juice, alfalfa, carrot powder, flax seed, bilberry, lecithin, milk thistle, royal jelly, ginkgo biloba, co-enzyme Q10, pine bark extract, pectin, bran, dom quai, ginseng, cinnamon and vegetable enzymes.
  • GREEN STUFF trade mark
  • French maritime bark extract is available under the trade mark PYCNOGENOL.
  • acetyl salicylic acid or its salts, e.g. acetyl salicylic acid.
  • An effective concentration is expected to be from about 0.5 to 4 grams acetyl salicylic acid per 200 litres.
  • the introduction of acetyl salicylic acid is believed to trigger the immune system of the animal .
  • One of the effects of the drink appears to be an ability to stabilize the pH of the body to neutral, i.e. a pH of about 7.
  • the present invention appears to enhance disease-resistance and enable the body to recuperate at a faster rate, especially when used in combination with compounds which are directed to alleviating or curing the disease.
  • the present invention can be used to help combat attention deficit disorders, and can be used to minimize or remove the need to rely on drug treatments such as methylphenidate (trade mark,
  • RITALIN RITALIN
  • Deficiencies of several essential fatty acids and, sometimes, vitamin B6 have been observed in children with attention deficit disorder. It is believed that intake of omega-3 and omega-6 fatty acids, along with the present aqueous solution would be helpful in combatting attention deficit disorder. Omega-3 and omega-6 fatty acids are available from flax seed. However, hemp seed gives a higher yield than flax seed. About 3 ounces hemp seed oil per 200 litres of solution is recommended.
  • Lettuce seeds were first soaked in colchicine solution before allowing to sprout. After sprouting the seedlings were transferred to rockwool cubes and placed on an ebb and flow hydroponic table.
  • the lettuce seedlings were continuously treated with a solution, described hereinafter, until the lettuce seeded.
  • the solution was made from a nutrient solution, a vitamin solution, a plant hormone and acetyl salicylic acid. These ingredients were combined with water into a 200 litre solution. The following quantities are the amounts in the 200 litre (50 U.S. gallon) solution.
  • the nutrients consisted of 165 g calcium nitrate, 22 g potassium sulphate, 87 g potassium nitrate, 26 g monopotassium phosphate, 12 g ammonium nitrate, 10 mL nitric acid and 3 g trace elements.
  • the B-vitamin mixture comprised 2000 meg vitamin B12, 500 mg vitamin B6, 500 mg vitamin B5, 200 mg vitamin B6 and 200 mg vitamin B2.
  • 30 mL (two tablespoons) LIQUINOX START (trade mark) solution which contained 2.00% phosphoric acid, 0.1% iron, 0.1% vitamin Bl and 0.04% ⁇ -naphthalene acetic acid, was also added.
  • Twenty five grams MICRO MIST 300 ascophyllum nodosum extract and 1 g acetyl salicylic acid were also added. Aphids were introduced after leaves appeared. The plants remained healthy.
  • Example 2 Example 2 :
  • Example 2 The experiment of Example 1 was repeated except that tomato seeds were used, and the nutrient solution contained 153 g calcium nitrate, 45 g potassium sulphate, 116 g potassium nitrate, 44 g monopotassium phosphate, 91 g magnesium sulphate, 10 mL nitric acid and 6 g trace elements.
  • Spider mites were introduced after leaves appeared. The plants remained healthy.
  • Example 4 The experiment of Example 1 was repeated except that cucumber seeds were used, and the nutrient solution contained 167 g calcium nitrate, 47 g potassium sulphate, 90 g potassium nitrate, 35 g monopotassium phosphate, 71 g magnesium sulphate, 6 g ammonium nitrate, 10 mL nitric acid and 6 g trace elements. The cucumbers were exposed to downy mildew and aphids but remained healthy.
  • Example 4 Example 4 :
  • Example 5 The experiment of Example 1 was repeated except that hemp seeds were used, and the nutrient solution contained 204 g calcium nitrate, 139 g potassium sulphate, 87 g monopotassium phosphate, 342 g magnesium sulphate and 28 g trace elements. This solution is particularly well adapted to production of flowers and seeds .
  • Example 5 Example 5:
  • Example 2 The experiment of Example 1 was repeated except that hemp seeds were used, and the nutrient solution contained 300 g calcium nitrate, 23 g potassium sulphate, 69 g monopotassium phosphate, 104 g potassium nitrate, 224 g magnesium sulphate and 20 g trace elements in 200 litres. To this solution was added 1 gram acetyl salicylic acid, 25 grams ascophyllum nodosum and two tablespoonsful (30 ml) of a solution of 2% phosphoric acid, 0.10% chelated iron, 0.1% vitamin Bl, 0.04% alpha naphthalene acetic acid with yucca extract. This solution is particularly well adapted to the growth of the hemp plants .
  • Example 6 Example 6 :
  • the seeds from Experiment 4 were taken and fed a growth solution.
  • the solution was made from a nutrient solution, a plant hormone, LIQUINOX START solution and acetyl salicylic acid. These ingredients were combined with water into a 200 litre solution. The following quantities are the amounts in the 200 litre solution and the nutrient solution contained 300 g calcium nitrate, 23 g potassium sulphate, 104 g potassium nitrate, 69 g monopotassium phosphate, 224 g magnesium sulphate and 20 g trace elements.
  • Example 7 The plants were vigorous and healthy and withstood infestation.
  • Example 7 The plants were vigorous and healthy and withstood infestation.
  • a first aqueous solution was prepared from 400 ml warm water, 1000 mg pantothenic acid, 400 mg riboflavin, 400 mg niacin, 500 mg pyridoxine, 2 g vitamin B12, two 500 mg multivitamin tablets, 800 IU vitamin D and 800 IU vitamin E.
  • a second solution was made with 25 ml LIQUINOX START, 1300 mg acetyl salicylic acid and 100 ml kelp.
  • the first and second solutions, and 1000 mg vitamin C were added to 200 litres of water to form a stock solution.
  • the stock solution had a pH of 7.0.
  • Mixture A comprising 3 teaspoonsful (about 15 ml) of calcium nitrate and 0.5 teaspoonsful (about 2.5 ml) of chelated trace elements was mixed in a 20 litre pail with Mixture B, comprising 0.125 teaspoonsful (about 0.625 ml) of potassium sulphate, 1.25 teaspoonsful (about 6.25 ml) of potassium nitrate, 0.75 teaspoonsful (about 3.75 ml) of monopotassium phosphate and 3 teaspoonsful (about 15 ml) of magnesium sulphate.
  • This solution had a pH of about 5.5.
  • the pail was then filled with stock solution and the resulting solution stirred.
  • the solution in the pail was split between two pails and each pail was topped up with water.
  • One of the pails was diluted 20:1 with water, and the other pail was diluted 10:1 with water.
  • Each of the diluted solutions pails had a pH of 7.0.
  • the ingredients in the diluted solutions were intended to provide a solution adapted for root development and general growth of the plants.
  • Solution A which comprised 4 litres of the new stock solution, 3 teaspoonsful (about 15 ml) of calcium nitrate and 1 teaspoonful (about 5 ml) of chelated trace elements was mixed in a 20 litre pail with Solution B, which comprised 4 litres of the new stock solution, 1 teaspoonful (about 5 ml) of potassium sulphate, 0.75 teaspoonsful (about 3.75 ml) of monopotassium phosphate and 4 teaspoonsful (about 20 ml) of magnesium sulphate.
  • the pail was then filled with new stock solution and the resulting solution stirred.
  • the solution in the pail was split between two pails and each pail was topped up with water.
  • One of the pails was diluted 20:1 and the other pail was diluted 10:1.
  • the diluted solutions had a pH of 7.0.
  • the crop of hemp was sprayed once with the 20:1 solution. Every 10 days thereafter, until the plants had bloomed and seeded, the hemp crop was sprayed with the 10:1 solution. The plants reached a height of about 3.5 to 3.7 metres, were vigorous, healthy and green in colour; and there was no yellowing as in a control crop which had not been sprayed with the solution.
  • Example 8 Two hundred litres of a drink solution were prepared with 153 g calcium citrate, 116 g potassium citrate, 91 g magnesium sulphate, 445 g potassium sulphate, 44 g monopotassium phosphate, 10 g trace elements, 28 g NITROZYME ascophyllum nodosum, 21 g LIQUINOX START solution (with ⁇ -naphthalene acetic acid) , 1 tablet MULTI-MINERAL PLUS (trade mark) multi minerals, 1000 meg vitamin B12 , 500 mg vitamin B6, 500 mg vitamin B5, 200 mg vitamin B3 , 200 mg vitamin B2 , 400 IU vitamin D, 1000 mg vitamin C, 1600 IU vitamin E oil, 1500 mg safflower oil, 1000 mg hemp oil, 1000 mg grape seed oil, 4 ml St. John's wort, 4 ml gingko biloba and 1200 mg acetyl salicylic acid.

Abstract

Cette solution aqueuse destinée au traitement de végétaux ou d'animaux contient, (i), un mélange d'éléments nutritifs à base de minéraux, (ii), un mélange de vitamines B, (iii), de l'acide acétylsalicylique ou un sel de celui-ci, et, (iv), au moins une hormone stimulant la croissance végétale ou un composé de type hormone. Parmi les hormones idoines figurent les cytokinines, les auxines, les gibbérellines, l'acide naphtalénique-α, l'acide naphtalène-α acétique et leurs mélanges, notamment de la cytokinine dérivée d'ascophyllum nodosum. Les éléments nutritifs à base de minéraux peuvent être des nitrates, des phosphates, des citrates et des sulfates de métaux alcalins, d'ammoniac, de métaux alcalino-terreux et de magnésium. Parmi les vitamines B, on peut noter les vitamines B1, B2, B6, B3, B12 et un complexe de la vitamine B. Les solutions, qui ont un pH neutre, peuvent être administrées à des végétaux par les racines ou les feuilles. Elle peuvent également être administrées à des animaux, à des humains notamment, sous forme buvable, par intraveineuse ou par injection.
EP99945792A 1999-09-24 1999-09-24 Methode permettant de renforcer l'immunite de vegetaux et d'animaux Withdrawn EP1217893A1 (fr)

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KR20010103157A (ko) * 2000-03-24 2001-11-23 이용환 식물병 방제물질로서 비타민 ｂ1의 신규한 용도
FR2826547B1 (fr) * 2001-06-29 2005-09-30 Agronomique Inst Nat Rech Utilisation en phytoprotection d'esters d'acide salicylique et de leurs associations avec des elicitines
JP3849025B2 (ja) * 2002-06-12 2006-11-22 国立大学法人広島大学 ビタミンｂ１２を含有する植物
GB0512336D0 (en) 2005-06-17 2005-07-27 Pi Bioscience Ltd Agricultural composition
GB0520726D0 (en) * 2005-10-12 2005-11-23 Plant Impact Plc Agricultural composition
EP2744346A4 (fr) * 2011-08-15 2015-12-16 Jr James Leahy Compositions et procédé de lutte contre les pathogènes foliaires fongiques
CN104542709B (zh) * 2015-01-13 2017-08-11 山西省农业科学院棉花研究所 小麦抗干热风制剂
ITUB20156248A1 (it) * 2015-12-04 2017-06-04 Alpha Biopesticides Ltd ?composition for crop care and protection? (?composizione per la cura e la protezione delle coltivazioni?)
FR3119514A1 (fr) 2021-02-08 2022-08-12 Agro Innovation International Composition pour la nutrition ou la boisson d’un animal non humain
CN113880632A (zh) * 2021-07-28 2022-01-04 安徽瑞邦生物科技有限公司 一种添加b族维生素的水溶型肥料

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CA2071767A1 (fr) * 1991-06-20 1992-12-21 Praveen K. Saxena Hyperproduction de pousses lors de la regeneration in vitro de plantes
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WO1999000016A1 (fr) * 1997-06-30 1999-01-07 Colegio De Postgraduados Utilisation de salicylates pour augmenter la bioproductivite chez les plantes
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