EP1194574A4 - Transfection tres efficace utilisant une faible intensite de champ electrique et une longue duree d'impulsion - Google Patents

Transfection tres efficace utilisant une faible intensite de champ electrique et une longue duree d'impulsion

Info

Publication number
EP1194574A4
EP1194574A4 EP99928868A EP99928868A EP1194574A4 EP 1194574 A4 EP1194574 A4 EP 1194574A4 EP 99928868 A EP99928868 A EP 99928868A EP 99928868 A EP99928868 A EP 99928868A EP 1194574 A4 EP1194574 A4 EP 1194574A4
Authority
EP
European Patent Office
Prior art keywords
electric field
high efficiency
field strength
pulse length
low electric
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
EP99928868A
Other languages
German (de)
English (en)
Other versions
EP1194574A1 (fr
Inventor
Ed Nolan
Robin Filshie
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genetronics Inc
Original Assignee
Genetronics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genetronics Inc filed Critical Genetronics Inc
Priority to EP07010185A priority Critical patent/EP1829973A3/fr
Publication of EP1194574A1 publication Critical patent/EP1194574A1/fr
Publication of EP1194574A4 publication Critical patent/EP1194574A4/fr
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
EP99928868A 1999-06-25 1999-06-25 Transfection tres efficace utilisant une faible intensite de champ electrique et une longue duree d'impulsion Ceased EP1194574A4 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP07010185A EP1829973A3 (fr) 1999-06-25 1999-06-25 Transfection à fort rendement selon une faible intensité de champ électrique et une faible longueur de l'impulsion

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US1999/014447 WO2001000856A1 (fr) 1999-06-25 1999-06-25 Transfection tres efficace utilisant une faible intensite de champ electrique et une longue duree d'impulsion

Related Child Applications (1)

Application Number Title Priority Date Filing Date
EP07010185A Division EP1829973A3 (fr) 1999-06-25 1999-06-25 Transfection à fort rendement selon une faible intensité de champ électrique et une faible longueur de l'impulsion

Publications (2)

Publication Number Publication Date
EP1194574A1 EP1194574A1 (fr) 2002-04-10
EP1194574A4 true EP1194574A4 (fr) 2003-04-02

Family

ID=22273056

Family Applications (2)

Application Number Title Priority Date Filing Date
EP07010185A Withdrawn EP1829973A3 (fr) 1999-06-25 1999-06-25 Transfection à fort rendement selon une faible intensité de champ électrique et une faible longueur de l'impulsion
EP99928868A Ceased EP1194574A4 (fr) 1999-06-25 1999-06-25 Transfection tres efficace utilisant une faible intensite de champ electrique et une longue duree d'impulsion

Family Applications Before (1)

Application Number Title Priority Date Filing Date
EP07010185A Withdrawn EP1829973A3 (fr) 1999-06-25 1999-06-25 Transfection à fort rendement selon une faible intensité de champ électrique et une faible longueur de l'impulsion

Country Status (4)

Country Link
EP (2) EP1829973A3 (fr)
AU (1) AU783432B2 (fr)
CA (1) CA2377565A1 (fr)
WO (1) WO2001000856A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60231625D1 (de) 2001-08-14 2009-04-30 Univ St Louis Systeme und verfahren zum screening pharmazeutischer chemikalien
WO2013075131A1 (fr) * 2011-11-17 2013-05-23 Brigham Young University Système de distribution de cytoplasme à un organite et procédés associés

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997007826A1 (fr) * 1995-08-29 1997-03-06 Cbr Laboratories, Inc. Electroporation in vivo de cellules
WO1999001158A1 (fr) * 1997-06-30 1999-01-14 Rhone-Poulenc Rorer S.A. Amelioration du transfert d'acide nucleique dans le muscle strie et combinaison permettant la mise en oeuvre du procede

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE68925030T2 (de) * 1988-01-21 1996-07-25 Massachusetts Inst Technology Molekültransport durch gewebe mit der verwendung von elektroporation.
US5944710A (en) * 1996-06-24 1999-08-31 Genetronics, Inc. Electroporation-mediated intravascular delivery
KR20010020570A (ko) * 1997-06-30 2001-03-15 자끄 사비나 다세포 진핵 생물 세포중으로 개선된 핵산 전달방법 및이를 위한 콤비네이션

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997007826A1 (fr) * 1995-08-29 1997-03-06 Cbr Laboratories, Inc. Electroporation in vivo de cellules
WO1999001158A1 (fr) * 1997-06-30 1999-01-14 Rhone-Poulenc Rorer S.A. Amelioration du transfert d'acide nucleique dans le muscle strie et combinaison permettant la mise en oeuvre du procede

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
AIHARA H ET AL: "GENE TRANSFER INTO MUSCLE BY ELECTROPORATION IN VIVO", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 16, no. 9, September 1998 (1998-09-01), pages 867 - 870, XP001061636, ISSN: 1087-0156 *
See also references of WO0100856A1 *

Also Published As

Publication number Publication date
AU4584299A (en) 2001-01-31
CA2377565A1 (fr) 2001-01-04
EP1194574A1 (fr) 2002-04-10
AU783432B2 (en) 2005-10-27
EP1829973A2 (fr) 2007-09-05
WO2001000856A1 (fr) 2001-01-04
EP1829973A3 (fr) 2008-01-09

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Legal Events

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PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

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