EP1194574A4 - High efficiency transfection based on low electric field strength, long pulse length - Google Patents

High efficiency transfection based on low electric field strength, long pulse length

Info

Publication number
EP1194574A4
EP1194574A4 EP99928868A EP99928868A EP1194574A4 EP 1194574 A4 EP1194574 A4 EP 1194574A4 EP 99928868 A EP99928868 A EP 99928868A EP 99928868 A EP99928868 A EP 99928868A EP 1194574 A4 EP1194574 A4 EP 1194574A4
Authority
EP
European Patent Office
Prior art keywords
electric field
high efficiency
field strength
pulse length
low electric
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
EP99928868A
Other languages
German (de)
French (fr)
Other versions
EP1194574A1 (en
Inventor
Ed Nolan
Robin Filshie
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genetronics Inc
Original Assignee
Genetronics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genetronics Inc filed Critical Genetronics Inc
Priority to EP07010185A priority Critical patent/EP1829973A3/en
Publication of EP1194574A1 publication Critical patent/EP1194574A1/en
Publication of EP1194574A4 publication Critical patent/EP1194574A4/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
EP99928868A 1999-06-25 1999-06-25 High efficiency transfection based on low electric field strength, long pulse length Ceased EP1194574A4 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP07010185A EP1829973A3 (en) 1999-06-25 1999-06-25 High efficienty transfection based on low electric field strength, low pulse length

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US1999/014447 WO2001000856A1 (en) 1999-06-25 1999-06-25 High efficiency transfection based on low electric field strength, long pulse length

Related Child Applications (1)

Application Number Title Priority Date Filing Date
EP07010185A Division EP1829973A3 (en) 1999-06-25 1999-06-25 High efficienty transfection based on low electric field strength, low pulse length

Publications (2)

Publication Number Publication Date
EP1194574A1 EP1194574A1 (en) 2002-04-10
EP1194574A4 true EP1194574A4 (en) 2003-04-02

Family

ID=22273056

Family Applications (2)

Application Number Title Priority Date Filing Date
EP99928868A Ceased EP1194574A4 (en) 1999-06-25 1999-06-25 High efficiency transfection based on low electric field strength, long pulse length
EP07010185A Withdrawn EP1829973A3 (en) 1999-06-25 1999-06-25 High efficienty transfection based on low electric field strength, low pulse length

Family Applications After (1)

Application Number Title Priority Date Filing Date
EP07010185A Withdrawn EP1829973A3 (en) 1999-06-25 1999-06-25 High efficienty transfection based on low electric field strength, low pulse length

Country Status (4)

Country Link
EP (2) EP1194574A4 (en)
AU (1) AU783432B2 (en)
CA (1) CA2377565A1 (en)
WO (1) WO2001000856A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE426015T1 (en) 2001-08-14 2009-04-15 Univ St Louis SYSTEMS AND METHODS FOR SCREENING PHARMACEUTICAL CHEMICALS
WO2013075131A1 (en) * 2011-11-17 2013-05-23 Brigham Young University Cytoplasm to organelle delivery system and associated methods

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997007826A1 (en) * 1995-08-29 1997-03-06 Cbr Laboratories, Inc. In vivo electroporation of cells
WO1999001158A1 (en) * 1997-06-30 1999-01-14 Rhone-Poulenc Rorer S.A. Improved method for transferring nucleic acid into the striped muscle and combination therefor

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0398960B1 (en) * 1988-01-21 1995-12-06 Massachusetts Institute Of Technology Transport of molecules across tissue using electroporation
US5944710A (en) * 1996-06-24 1999-08-31 Genetronics, Inc. Electroporation-mediated intravascular delivery
JP4664450B2 (en) * 1997-06-30 2011-04-06 アンステイテユ・ギユスタブ・ルシー Improved methods for introducing nucleic acids into multicellular eukaryotic cells and combinations thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997007826A1 (en) * 1995-08-29 1997-03-06 Cbr Laboratories, Inc. In vivo electroporation of cells
WO1999001158A1 (en) * 1997-06-30 1999-01-14 Rhone-Poulenc Rorer S.A. Improved method for transferring nucleic acid into the striped muscle and combination therefor

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
AIHARA H ET AL: "GENE TRANSFER INTO MUSCLE BY ELECTROPORATION IN VIVO", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 16, no. 9, September 1998 (1998-09-01), pages 867 - 870, XP001061636, ISSN: 1087-0156 *
See also references of WO0100856A1 *

Also Published As

Publication number Publication date
EP1829973A2 (en) 2007-09-05
WO2001000856A1 (en) 2001-01-04
EP1829973A3 (en) 2008-01-09
AU4584299A (en) 2001-01-31
EP1194574A1 (en) 2002-04-10
CA2377565A1 (en) 2001-01-04
AU783432B2 (en) 2005-10-27

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Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20020118

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

A4 Supplementary search report drawn up and despatched

Effective date: 20030218

17Q First examination report despatched

Effective date: 20050613

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION HAS BEEN REFUSED

18R Application refused

Effective date: 20070524