EP1185368A1 - Pipette with tip ejector - Google Patents
Pipette with tip ejectorInfo
- Publication number
- EP1185368A1 EP1185368A1 EP99917735A EP99917735A EP1185368A1 EP 1185368 A1 EP1185368 A1 EP 1185368A1 EP 99917735 A EP99917735 A EP 99917735A EP 99917735 A EP99917735 A EP 99917735A EP 1185368 A1 EP1185368 A1 EP 1185368A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- cylinder
- pipette
- ejection member
- ejection
- piston
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/0275—Interchangeable or disposable dispensing tips
- B01L3/0279—Interchangeable or disposable dispensing tips co-operating with positive ejection means
Definitions
- the invention relates to a pipette for transferring liquids, comprising at least one piston-cylinder assembly arranged so that it can be provided with a transfer nozzle.
- Pipettes of this type are generally known, an example being described in American patent US Pat. No. 5,614,153.
- Such a pipette allows small amounts of liquid to be transferred into a replaceable tip mounted at the free end of the pipette cylinder. This transfer takes place without contamination of the pipette, the used tips being replaced by new tips.
- the pipette is provided with a nozzle ejector which can be actuated manually and comprises an ejection member sliding along the cylinder to abut against the nozzle and push it so as to separate it from the cylinder. In this pipette, the ejection member travels an invariable distance and its final position is fixed.
- the tips used can come from different manufacturers or have different shapes, so their dimensions can vary from one to the other.
- the position of the tips on the cylinder can differ depending on the variants available on the market and depending on the engagement force. It follows a variation in the position of the edge of the tip on which abuts the ejection member.
- the position variations being of the order of magnitude of the stroke of the ejection member, certain end pieces are not ejected or can abut on the ejector before creating a seal between the end piece and the cylinder of the pipette.
- An object of the invention is to provide a pipette which makes it possible to attach and eject the tips in a reliable manner, in particular taking into account the variable position of the edge of the tip relative to the pipette.
- a pipette is preferably designed so as to be easy to use and robust.
- the present pipette for transferring liquids comprises at least one piston-cylinder assembly, the cylinder of which may be provided with a replaceable tip, a piston actuation device for causing suction. and the liquid delivery and a nozzle ejector device comprising an ejection member movable along the cylinder, has the particularity that the ejector device comprises a position adjustment device arranged so as to allow the limit position reached to be modified at the end of the stroke by the end of the ejection member during an ejection operation from the end piece. It should be noted that in practice, the adjustment will generally be made by adjusting the starting position of the free edge of the ejection member intended to come up against the upper edge of the end piece. In all embodiments, the present pipette allows attaching and ejecting, reliably, tips of different shapes and sizes.
- the position adjustment device may include a sleeve disposed on the cylinder of the piston-cylinder assembly, the cooperation between this sleeve and the ejection member making it possible to adjust the position of the end of the ejection from the cylinder.
- the socket and the ejection member can be coupled by threaded parts or by the support of a lug or another element against a crown having an inclined surface, so that the adjustment of the position of the member d ejection takes place by a relative rotation between the sleeve and this member.
- the ejection member is rotatable and the socket is kept fixed
- the socket is rotary and the ejection member is fixed.
- the socket and / or the ejection member may be provided with notches, a lug or similar means for defining its angular position relative to the counterpart.
- the sleeve which can be mounted on a part of the cylinder, and the ejection member are easily accessible and adjustable.
- Variants of the position adjustment device consist in using, for example, a socket whose position is adjustable relative to the ejection member and which is mounted on this member so as to form the ejection end of this one.
- the sleeve and the ejection member may have respective threaded parts, or may comprise other fixing means making it possible to adjust the position of the sleeve as a function of the endpiece used.
- Another variant consists in using a position adjustment device which makes it possible to modify the limit position of the ejection member by a variation of the stroke of the ejection member relative to the cylinder.
- the ejection member can for example be coupled to its actuating device via a member of variable length so as to modify at least one limit position of the actuating member.
- Fig. 1 is a partial sectional view of a first embodiment of a pipette according to the invention
- Fig. 2 is a partial sectional view of a piston-cylinder assembly of the pipette of FIG. 1;
- Fig. 3 is an enlarged view, in section, along the line III-III of FIG. 2;
- Fig. 4 is a sectional view of a second embodiment of a pipette according to the invention.
- Fig. 5 is a partial sectional view of a piston-cylinder assembly of a third embodiment.
- Figures 6 to 9 are partial views, in section, of variants of a position adjustment device disposed near the end of the pipette cylinder.
- FIGS. 1 to 3 and Fig. 4 show, respectively, pipettes 1 and 1 ′, the analogous parts of which have been designated by the same reference numeral.
- a pipette comprises a pipette body 2 provided with a usual piston-cylinder assembly 3, 4, a piston actuation device 5 and an ejector device 6.
- the piston actuation device 5 comprises a pusher 7 acting on the piston 3 of the piston-cylinder assembly which makes it possible to aspirate and / or pumping liquid through a replaceable nozzle 8 mounted at the end 9 of the cylinder 4 of the piston-cylinder assembly.
- the cylinder 4 communicates with the end piece 8 through an opening 10 at its end 9.
- the plunger 7 of the piston actuation device 5 comprises a button 11 and a rod 12.
- the rod 12 is coupled to the button 11 by means of a threaded part 13, so as to allow the position of the rod 12 and therefore of the piston 3 relative to the cylinder to determine the volume of liquid sucked.
- Other devices for actuating the piston 12 can be provided without departing from the scope of the invention, the actuation possibly being, for example, hydraulic or electric.
- the rod 12 is coupled at its end 14 in a separable manner to the piston 3.
- the piston 3 is provided with elastic arms 15 having recesses 56 on their internal surface so as to be able to cooperate with the end 14 of the rod 12, which is shaped like a ball.
- the coupling between the elastic arms 15 and the end 14 is therefore quickly separable by application of a certain tensile force on the piston-cylinder assembly relative to the body 2. This makes it possible, for example, to exchange the piston assemblies -cylinder having different suction volumes or of different shape and dimensions to comply with different standards, or to exchange defective assemblies without having to replace the entire device.
- the connection between the elastic arms 15 and the ball 14 form an advantageous plug and socket assembly because it is very simple and quick to disconnect and reconnect the devices.
- the ejector device 6 comprises an ejection member 16 or 16 ′ having a tubular part arranged so as to be able to slide on the cylinder 4, and an actuating device 17 of this ejection member.
- the actuating device 17 comprises a pusher 18 and a rod 19 connecting the pusher to a coupling member 20 or 20 '.
- the coupling member makes a separable connection between the ejection member 16 or 16 'and the actuating device 17 by snap-fastening, for example by the interaction of protuberances and grooves formed on complementary parts.
- the ejection member 16 or 16 ′ is moved over a distance determined by the movement of this pusher.
- the end 22 of the ejection member abuts on the upper edge 23 of the nozzle to separate it from the cylinder 4.
- the pusher 18 is advantageously placed on the same side of the pipette body as the claw 21, this which facilitates the actuation of the ejection pusher easily accessible to the operator.
- this arrangement makes the opposite side of the pipette body free to easily arrange a display device there.
- the ejector device comprises a position adjustment device 24 comprising a threaded part 25 at the upper end of the rod 19 which is engaged in a threaded part complementary to a bore 26 of the pusher 18.
- the head of the part threaded 25 is accessible from the outside to allow adjustment of the position of the rod 19 relative to the pusher 18, the coupling member 20 allowing the rotation of the rod 19.
- This rotation displaces the ejection member 16 relative to the cylinder 4.
- the position of the end 22 of the ejection member 16 can therefore be adjusted to allow the ejection of end pieces 8 having upper edges situated at different heights relative to the end 9 of the cylinder.
- the position of the pusher 18 is adjusted to vary the stroke of the actuating member determined by the distance between the upper limit position of the pusher and the sleeve or the claw 21.
- the variation of the stroke of the pusher 18 modifies thus also the limit position of the end 22 of the ejection member reached during actuation of the ejector. This produces a reliable ejection of tips having different positions and / or dimensions.
- Figures 1 to 3 show a preferred embodiment of the invention.
- the position adjustment device 24 ′ comprises a socket 27 mounted around the cylinder 4 near the upper end of the latter.
- the socket 27 has a peripheral groove 28 into which a projecting part of the coupling member 20 'connected to the actuating rod 19 penetrates.
- the socket 27 can rotate around its axis, while being integral, in the axial direction, with the actuating device of the ejection member 16 '.
- An internal thread 29 of the sleeve 27 cooperates with a thread 30 on the upper part of the ejection member 16 ', so that a rotation of the sleeve makes it possible to move the end 22 of the ejection member away from or closer to the end 9 of the cylinder 4.
- the inner surface of the sleeve has a series of axial notches 31 in which a godron 32 of the periphery of the upper part of the member 16 'can engage so as to define the angular position of the sleeve with respect to to this organ.
- the member 16 ' is prevented from rotating about its axis and therefore moves in the axial direction relative to the cylinder 4 when adjusting its position by rotation of the sleeve 27.
- the sleeve 27 is fixed and the member 16 'is rotatably mounted, so that a rotation of the ejection member makes it possible to move the end 22 away from or closer to the end 9 of the cylinder 4.
- the ejector device comprises a position adjustment device 24 "provided with a fixed socket 27 'and an ejection member 16" mounted so as to allow its rotation on the cylinder 4.
- the member ejection is provided with a part 32 with a lug 32 "which engages in notches 31 'of a crown 29' having an annular inclined engagement surface 29".
- the notches 31 ′ arranged at intervals around the periphery of the crown thus define different height positions for adjusting the position of the end 22 of the ejection member 16 ".
- a spring 55 acting on the bush 27 'and on the member 16 "ensures the engagement of the lug 32" in one of the notches 31'.
- the piston-cylinder device of FIG. 5 can also include a piston and means for allowing rapid coupling to a pipette body similar to the means described above in relation to the embodiment of Fig. 2.
- an adjustment sleeve can be mounted on an end part of the ejection member, according to the variants shown in Figures 6 to 9, to form an ejection edge 22 'whose position can be adjusted relative to cylinder 4 of the pipette.
- a socket 33 or 33 ' is mounted on the part 34 or 34' of the ejection member which has a thread 35 or 35 ' cooperating with a complementary thread of the sleeve.
- notching grooves 36 are formed on the bottom of the thread to allow a good definition of the angular position of the sleeve 33.
- the notching is moved on the cylindrical part 37 of the member ejection and formed by a set of notches-gadroons 38 on this part and the socket 33 '.
- Fig. 7 shows a variant in which a socket 39 is mounted on an end portion 40 of the ejection member by means of lugs, such as 41, which are positioned in corresponding grooves arranged on the periphery of the part 40.
- lugs such as 41
- These grooves such as 42, 43, 44, have different heights which make it possible to define corresponding positions of the sleeve relative to the ejection member.
- the guide of the sleeve is provided by the cylinder 4 on which it slides.
- a socket 39 ' is provided with longitudinal tabs 50, provided at their free ends with protrusions 51 arranged so as to be able to penetrate in dormers, such as 52, 53, 54, located at different heights in the part 40' of the ejection member.
- the limit position of the end of the ejection member is adjustable at this end so as to obtain an effective ejection for end pieces whose upper edge is located at different heights from the pipette cylinder.
Abstract
Description
Claims
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/CH1999/000210 WO2000069562A1 (en) | 1999-05-18 | 1999-05-18 | Pipette with tip ejector |
Publications (2)
Publication Number | Publication Date |
---|---|
EP1185368A1 true EP1185368A1 (en) | 2002-03-13 |
EP1185368B1 EP1185368B1 (en) | 2003-09-17 |
Family
ID=4551679
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP99917735A Expired - Lifetime EP1185368B1 (en) | 1999-05-18 | 1999-05-18 | Pipette with tip ejector |
Country Status (7)
Country | Link |
---|---|
US (1) | US6833114B1 (en) |
EP (1) | EP1185368B1 (en) |
AT (1) | ATE249885T1 (en) |
AU (1) | AU3593199A (en) |
DE (1) | DE69911474T2 (en) |
ES (1) | ES2207204T3 (en) |
WO (1) | WO2000069562A1 (en) |
Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2807342B1 (en) * | 2000-04-07 | 2002-07-12 | Gilson Sa | CONCEAL EJECTOR PIPETTE |
FR2807345B1 (en) * | 2000-04-07 | 2002-07-12 | Gilson Sa | SIMPLIFIED DISASSEMBLY PIPETTE |
FR2807340B1 (en) | 2000-04-07 | 2002-12-06 | Gilson Sa | PIPETTE FOR TAKING LIQUID SAMPLES WITH CONE EJECTOR MECHANISM |
FR2807341B1 (en) | 2000-04-07 | 2002-07-12 | Gilson Sa | PIPETTE FOR COLLECTING LIQUID SAMPLES WITH REMOVABLE EJECTOR |
EP1329262B1 (en) * | 2002-01-16 | 2009-08-19 | Brand Gmbh + Co Kg | Pipette and method of manufacturing a pipette |
FI20031683A0 (en) * | 2003-11-19 | 2003-11-19 | Thermo Electron Oy | Pipette with tip removal mechanism |
DE102004003433B4 (en) * | 2004-01-21 | 2006-03-23 | Eppendorf Ag | Pipetting device with a discharge device for pipette tips |
US7641859B2 (en) * | 2004-02-11 | 2010-01-05 | Matrix Technologies Corporation | Pipette tip mounting and ejection assembly and associated pipette tip |
ES2330948T3 (en) * | 2005-02-04 | 2009-12-17 | F. Hoffmann-La Roche Ag | PIPETTE FOR DISPOSABLE TIPS OF DIFFERENT SIZE. |
US20090071267A1 (en) * | 2007-09-17 | 2009-03-19 | Greg Mathus | Pipette tip ejection mechanism |
PL233500B1 (en) * | 2011-05-31 | 2019-10-31 | Pz Htl Spolka Akcyjna | Pipette for use with a pipette tip |
FI123652B (en) * | 2011-10-20 | 2013-08-30 | Thermo Fisher Scientific Oy | Lockable pipette |
US9815053B2 (en) * | 2013-01-15 | 2017-11-14 | Mettler-Toledo Rainin, LLC | Liquid end assembly for a multichannel air displacement pipette |
CN103769254B (en) * | 2014-01-10 | 2015-12-16 | 浙江大学 | Harmless cultivation picks up transfer device and method thereof |
USD963886S1 (en) * | 2021-03-12 | 2022-09-13 | Mettler-Toledo Rainin, LLC | Pipette body |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2287941A1 (en) * | 1974-10-15 | 1976-05-14 | Marteau D Autry Eric | DEVICE FOR EJECTING THE REMOVABLE TIP OF A PIPETTE |
US4965050A (en) * | 1989-01-18 | 1990-10-23 | Ballard Medical Products | Adaptation of pipetter |
GB2249172A (en) * | 1990-10-26 | 1992-04-29 | N Proizv Ob Biolog Priborostro | Multichannel pipette |
WO1996037302A1 (en) * | 1995-05-26 | 1996-11-28 | Rainin Instrument Co., Inc. | Pipette tip ejector |
DE19708151A1 (en) * | 1997-02-28 | 1998-09-10 | Eppendorf Geraetebau Netheler | Pipette |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4009611A (en) * | 1976-05-13 | 1977-03-01 | Oxford Laboratories Inc. | Hand-held micropipettor with improved pipette tip ejector |
US5320810A (en) * | 1992-05-13 | 1994-06-14 | Integrated Instrument Services, Inc. | Pipette with an axially stationary volume adjusting wheel |
FR2807342B1 (en) * | 2000-04-07 | 2002-07-12 | Gilson Sa | CONCEAL EJECTOR PIPETTE |
US6749812B2 (en) * | 2000-06-26 | 2004-06-15 | Vistalab Technologies | Automatic pipette detipping |
EP1438138B1 (en) * | 2001-10-16 | 2010-09-08 | Matrix Technologies Corporation | Hand-held pipettor |
EP1329262B1 (en) * | 2002-01-16 | 2009-08-19 | Brand Gmbh + Co Kg | Pipette and method of manufacturing a pipette |
-
1999
- 1999-05-18 EP EP99917735A patent/EP1185368B1/en not_active Expired - Lifetime
- 1999-05-18 AU AU35931/99A patent/AU3593199A/en not_active Abandoned
- 1999-05-18 US US09/979,323 patent/US6833114B1/en not_active Expired - Lifetime
- 1999-05-18 WO PCT/CH1999/000210 patent/WO2000069562A1/en active IP Right Grant
- 1999-05-18 AT AT99917735T patent/ATE249885T1/en active
- 1999-05-18 ES ES99917735T patent/ES2207204T3/en not_active Expired - Lifetime
- 1999-05-18 DE DE69911474T patent/DE69911474T2/en not_active Expired - Lifetime
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2287941A1 (en) * | 1974-10-15 | 1976-05-14 | Marteau D Autry Eric | DEVICE FOR EJECTING THE REMOVABLE TIP OF A PIPETTE |
US4965050A (en) * | 1989-01-18 | 1990-10-23 | Ballard Medical Products | Adaptation of pipetter |
GB2249172A (en) * | 1990-10-26 | 1992-04-29 | N Proizv Ob Biolog Priborostro | Multichannel pipette |
WO1996037302A1 (en) * | 1995-05-26 | 1996-11-28 | Rainin Instrument Co., Inc. | Pipette tip ejector |
DE19708151A1 (en) * | 1997-02-28 | 1998-09-10 | Eppendorf Geraetebau Netheler | Pipette |
Also Published As
Publication number | Publication date |
---|---|
ES2207204T3 (en) | 2004-05-16 |
ATE249885T1 (en) | 2003-10-15 |
DE69911474T2 (en) | 2004-07-22 |
EP1185368B1 (en) | 2003-09-17 |
DE69911474D1 (en) | 2003-10-23 |
AU3593199A (en) | 2000-12-05 |
WO2000069562A1 (en) | 2000-11-23 |
US6833114B1 (en) | 2004-12-21 |
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