EP1135210B1

EP1135210B1 - Pipette with improved pipette tip and mounting shaft combination

Info

Publication number: EP1135210B1
Application number: EP99971738A
Authority: EP
Inventors: James S. Petrek
Original assignee: Rainin Instrument Co Inc
Current assignee: Rainin Instrument Co Inc
Priority date: 1998-11-06
Filing date: 1999-11-05
Publication date: 2010-07-21
Anticipated expiration: 2019-11-05
Also published as: TW431912B; EP1135210A1; PL366207A1; EP1135210A4; CN1146473C; WO2000027528A1; CN1348397A; PL192753B1; BR9915086A

Abstract

An air displacement pipette (10) having axially spaced annular sealing and frusto-conical lateral support zones (36, 38) and regions (46, 48) on the pipette"s mounting shaft (32) and tip (40), respectively, in combination with means (52) for insuring uniform depth of mounting shaft penetration into the pipette tip to maintain uniform tip interference with the mounting shaft as successive tips are mounted on and ejected from the mounting shaft whereby the pipette tip is easily and firmly mountable on and ejectable from the pipette tip mounting shaft by the application of reduced user generated axial forces.

Description

The present invention relates to an air displacement pipette in accordance with the preamble of claim 1. An air displacement pipette of this kind is disclosed in US-A-4,824,641 . US-A-4,824,641 is directed to an automated sample handling device where the problems related to repetitive stress injury and possible rocking of the tip on the mounting shaft during "touching off" do not arise. Furthermore, as stated in the said US-A-4,824,641 , there is substantial resistance to mounting of the tip on the pipette because of the relative angles of the taper and the seal surface. The seal is formed not at the open proximal end of the tip but adjacent to a step transition between the proximal and distal ends of the tip which is relatively very stiff and thus increases the forces needed for mounting and ejecting of the tip.
Reference should also be made to US-A-5,200,151 . This reference also relates to an automatic pipetting system where again there is no need to pay attention to low force levels or to the special requirements of touching off. The reference is actually directed to ensuring that the relative (vertical) position of the tip orifice and of the holder (pipette) on which the tip is carried is always accurately established.
In this design a lip at the bottom of the proximal portion of the tip that engages the bottom surface of the pipette shaft and provides a seal which blocks all flow of air from the tip bowl into the tip chamber. Thus, the seal is again provided adjacent the horizontal step in the tip which is undesirable from a force consideration.
It is the object of the present invention to provide an improved air displacement pipette including a unique pipette tip tailored to the distal end of the pipette's tip mounting shaft such that the tip is easily insertable by a pipette user onto the mounting shaft to a fluid tight position in which the tip is secured against undesired lateral rocking on or displacement from the shaft and, after use, is easily ejectable from the shaft by the pipette user.
During the development of the unique pipette tip described in United States patent application serial number 09/188,030, filed November 6,1998 and entitled "Easy Eject Pipette Tip" and the development of the novel pipette tip and tip mounting shaft combination described in United States patent application serial number 09/188,031, filed November 6,1998 and entitled "Pipette With Improved Pipette Tip and Mounting Shaft", both applications being incorporated herein by this reference, a special pipette tip and mounting shaft combination, namely the combination underlying the present invention, was discovered which provides for easier tip mounting, improved lateral tip stability and easier tip ejection than standard commercially available pipette tip and tip/shaft combination.
Thus, in order to satisfy the above object, there is provided an air displacement pipette of the above kind which is characterized by

(i) the annular sealing region of the tip and the annular sealing zone of the shaft having a frusto-conical shape, with the outer surface of the frusto-conical distal end of the shaft being substantially parallel to the inner frusto-conical surface of the proximal end portion of the tip,
(ii) the sealing region of the tip being disposed adjacent the open proximal end of the tip and nearer to said open proximal end than is said lateral support region ,
(iii) said sealing region being formed by an inwardly extending bead extending radially inward from the sidewall of the pipette tip to form an axially narrow inner sealing band or line, the outer diameter of said annular sealing zone of the shaft being slightly greater than the inner diameter of the annular sealing region of the tip and the sidewall of the tip in the annular sealing region being made sufficiently thin that the sealing region expands slightly to form an interference fit and said airtight seal,
(iv) the lateral support region of the tip having a frusto-conical inner surface that cooperates with a frusto-conical support zone on the shaft, with the outer diameters of the shaft in the axially tapering lateral support zone being slightly less than or substantially equal to corresponding inner diameters of the proximal end portion of the tip n at least some circumferential portions of the axially tapering lateral support region whereby to provide lateral support for the tip that prevents transverse rocking of the tip on the shaft as might otherwise occur during touching off of the pipette tip on the mounting shaft, without creating an air-tight seal between the tip and the shaft.

Thus, the present invention incorporates in an air displacement pipette the concept of similar frusto-conical distal and proximal end portions on a pipette tip mounting shaft and pipette tip respectively. The frusto-conical distal and proximal end portions have substantially parallel inwardly and downwardly tapering surfaces incorporating axially spaced annular sealing and lateral support zones and regions on the mounting shaft and tip, respectively. Further, the present invention incorporates cooperative means on the shaft and tip for insuring uniform depth of mounting shaft penetration into the pipette tip to maintain uniform tip interference with the mounting shaft as successive tips are mounted on and ejected from the mounting shaft.
Thus, the present teaching relates to a combination of a pipette tip mounting shaft and pipette tip in an air displacement pipette. The mounting shaft comprises an axially elongated body including a frusto-conical distal end portion having an axially tapering outer surface with annular axially spaced outer surface regions defining an annular sealing zone and an axially tapering annular lateral support zone. The pipette tip is an elongated tube comprising an open frusto-conical proximal end portion, an open conical distal end and annular axially spaced inner surface regions on the axially tapering frusto-conical inner surface of the proximal end portion defining an annular sealing region and an axially tapering annular lateral support region. The axial tapering of the mounting shaft and pipette tip are one and one-half degrees or more from the longitudinal axis of the shaft and preferably between one and one-half and five or six degrees. The outer diameter of the annular sealing zone on the mounting shaft is slightly greater than the inner diameter of the annular sealing region on the pipette tip and the sidewall of the tip in the area of the annular sealing region is sufficiently thin that the annular sealing region expands slightly to form an interference fit and air tight seal between the mounting shaft and the pipette tip when the sealing zone penetrates the sealing region. The axial spacing of the sealing and support zones is substantially equal to the axial spacing of the sealing and support regions. Also, the outer diameters of the mounting shaft in the axially tapering lateral support zone are slightly less than or substantially equal to corresponding inner diameters of the proximal end portion of the tip in at least some circumferential portions of the axially tapering lateral support region such that, as the sealing zone penetrates the sealing region, this allows for some minimal contact between the support zone and region without creating a secondary air tight seal which would result in an undesired increase in the axial forces required to mount and eject the pipette on and from the shaft. With such a structural configuration, as the sealing zone penetrates the sealing region, the support region receives the support zone and provides lateral support therefor which prevents transverse rocking of the pipette tip on the mounting shaft as might otherwise occur during touching off of the pipette tip and an accompanying undesired dislodging of the tip from the shaft.
Further, the preferred embodiment of the present invention includes the aforementioned controlled interference air tight fit and mating annular lateral support zone and region as well as cooperative means on the pipette and pipette tip for limiting the axial travel of the tip on the mounting shaft. This insures uniform depth of mounting shaft penetration into the pipette tip to maintain uniform the desired tip interference with the mounting shaft as successive tips are mounted on and ejected from the mounting shaft.
Still further, for pipette tip and shaft combinations wherein the interference fit between the sealing zone and region is about .075 mm to about .2 mm and the wall thickness of the pipette tip in the sealing region is between .2 and .5 mm, it has been discovered that the desired minimal tip mounting and ejection forces associated with the present invention still may be achieved and the lateral stability of the tip on the shaft further enhanced when there is a small interference fit between the support region and zone. The small interference is provided by the lateral support region of the tip having an inner diameter which is slightly less than the outer diameter of the lateral support zone of the shaft, e.g. less than .075 mm.
The invention will now be described in more detail by way of example with reference to the accompanying drawings in which

Fig. 1 is a side view of a standard prior art manual pipette having a pipette tip mounted on a mounting shaft adjacent a lower end of a tip ejector mechanism of the pipette.
Fig. 2 is a cross sectional side view of one embodiment of the pipette tip and mounting shaft combination of the present invention showing the fluid tight seal between the sealing region and sealing zone, the mating relationship of the lateral support region and zone and a preferred embodiment of the cooperative means including a shoulder on the pipette tip for limiting mounting shaft penetration into the tip.
Fig. 3 is an enlarged fragmentary section side view of the sealing region within the circle 3 for the pipette tip of Fig. 2.
Fig. 4 is an enlarged fragmentary side view of an upper portion of the pipette tip and mounting shaft combination similar to Fig. 2 showing a first alternative embodiment of the cooperative means including a shoulder on the mounting shaft for limiting mounting shaft penetration into the tip.

Turning now to the figures, Fig. 1 illustrates a standard manual pipette resembling the PIPETMAN pipette sold exclusively in the United States by the Rainin Instrument Co. Inc., assignee of the present invention. The manual pipette is designated in Fig. 1 by the number 10 and includes a pipette tip ejector mechanism 12 described in United States patent 3,991,617 issued November 16, 1976 , which is incorporated herein by this reference.
The pipette 10 comprises a push button 14 connected by a rod 16 to a piston (not shown) located in the body or housing 18 of the pipette. The push button 14 may be depressed by a user exerting a downward force on the push button to cause downward movement of the piston of the pipette. When the push button 14 is released, a quantity of liquid to be sampled is sucked into a disposable pipette tip 20 releasably secured to a lower end of a pipette tip mounting shaft 22 of the pipette. The sample then may be transferred into another vessel by once more exerting a downward force on the push button 14. After such use, it is common practice to eject the pipette tip 20 from the mounting shaft 22 and replace it with a new pipette tip for repeated operation of the pipette 10 in aspirating and dispensing a new sample fluid.
The pipette tip mechanism ejector 12 is employed to eject the tip 20 from the mounting shaft 22. In this respect, the mechanism 12 comprises a push button 24 connected to a rod located in a passage (not shown) provided in an upper part of the hand holdable housing 18 of the pipette 10. The passage and rod are arranged so as to be able to impart to the rod a movement of translation parallel to an axis of the pipette in opposition to a spring (not shown) normally urging the rod in an upward position. A removable tip ejector member or arm 26 including a tubular upper end extends from a lower end of the rod and from the rod follows the general exterior contour of the housing 18 of the pipette to terminate in a sleeve 28. The sleeve 28 encircles a conical lower end 30 of the pipette tip mounting shaft 22 which tightly receives the upper end of the disposable pipette tip 20. To eject the pipette tip 20 from the lower end of the mounting shaft 22, a user grips the pipette housing 18 and using his or her thumb presses downward on the push button 24. The downward force on the push button is translated by the rod to the tip ejector arm 26 and hence to the sleeve 28 which presses down on an upper end of the pipette tip. When the downward force transferred by the sleeve 28 exceeds the friction between the pipette tip 20 and the mounting shaft 22, the pipette tip is propelled from the mounting shaft. Upon a release of the push button 24, the spring returns the tip ejector mechanism 12 to its normal position with the sleeve spaced slightly from the upper end of a replacement pipette tip which is inserted onto the mounting shaft 22 readying the pipette 10 for its next aspiration and dispensing operation.
A preferred embodiment of the structure of the pipette tip and mounting shaft combination of the present invention is depicted in Fig. 2. As there illustrated, the mounting shaft 32 comprises an axially elongated body including a frusto-conical distal end portion 34 having an outer surface which tapers axially inwardly from a main or upper portion of the shaft. The axially tapering outer surface of the distal end portion 34 comprises annular axially spaced outer surface regions defining an annular sealing zone 36 adjacent an upper end of the distal end 34 and an annular axially and downwardly and inwardly tapering lateral support zone 38 on the distal end portion 34 near the lower end of the mounting shaft 32.
The pipette tip is represented by the numeral 40 and is an elongated plastic tube comprising an open frusto-conical proximal end portion 42, an open conical distal end portion 44 and annular and axially spaced inner surface regions on the axially downwardly and inwardly tapering inner surface 43 of the proximal end portion 42 defining an annular sealing region 46 and an axially tapering annular lateral support region 48 for mating with the sealing and support zones 36 and 38 respectively, on the mounting shaft 32. As illustrated, the frusto-conical inner surface of the proximal end portion 42 of the tip 40 is similar to and slightly larger than the frusto-conical outer surface of the distal end portion 34 of the shaft 32. Also, in any axial vertical plane, the outer surface of the frusto-conical distal end portion of the shaft 32 is substantially parallel to the inner frusto-conical surface of the proximal end portion 42 of the tip 40. As used herein, "substantially parallel" means that the outer surface of the axially tapering outer surface of the distal end portion 34 is within one and one-half degrees of the axial taper of the inner surface 43 of the proximal end portion 42 of the tip 40.
Fig. 3 illustrates in enlarged detail a preferred embodiment of the sealing region 46 and comprises the portion of the pipette tip 40 of Fig. 2 within the circle 3. As shown, the sealing region 46 is formed by an inwardly extending substantially V-shaped bead 49 extending radially inward from the sidewall 50 of the pipette tip 40. The innermost surface of the bead 49 forms a very narrow annular sealing band or line for engaging the sealing zone 36 of the pipette tip mounting shaft 32 to form the previously described air-tight seal between the tip and mounting shaft.
As illustrated in Fig. 2, the outer diameter of the annular sealing zone 36 is slightly greater than the inner diameter of the annular sealing region 46 on the pipette tip 40 and the sidewall 50 of the tip in the area of the annular sealing region 46 is sufficiently thin that the annular sealing region expands slightly to form an interference fit and air tight seal between the mounting shaft 32 and the pipette tip 40 when the sealing zone 36 penetrates the sealing region 46. In practice, it has been found that the desired interference fit is formed when the difference in the outer diameter of the annular sealing zone and the inner diameter of the annular sealing region is at least .075 millimeters (mm). Further, it has been found in practice the wall thickness of the pipette tip in the area of the sealing region 46 is preferably between .20 and .50 mm.
Also as illustrated in Fig. 2, the axial spacing of the sealing zone and region is substantially equal to the axial spacing of the support zone and region. Also, the outer diameters of the shaft 32 within the axially tapering lateral support zone 38 are slightly less than or substantially equal to corresponding inner diameters of the proximal end portion within at least some circumferential portions of the axially tapering lateral support region 48. This allows for some minimal contact between the support zone and region without creating a secondary air tight seal which would result in an undesired increase in the axial forces required to mount and eject the pipette tip on and from the shaft. With such a structural configuration, as the sealing zone 36 penetrates the sealing region 46, the support region 48 receives the support zone 38 and provides lateral support therefor which prevents transverse rocking of the pipette tip 40 on the mounting shaft 32 as might otherwise occur during "touching off" of the pipette tip and an accompanying undesired dislodging of the tip from the shaft. In these regards, it is preferred that the axial spacing of the mating lateral support zone 38 and region 48 from the sealing zone and region (36,46) is substantially equal to the inner diameter of the pipette tip 40 in the portion of the support region engaging the support zone. Such a length relationship provides excellent lateral stability for the pipette tip 40 on the mounting shaft 32.
Further, as illustrated in Fig. 2, the present invention includes cooperative means 52 on the pipette of the present invention and the pipette tip 40 for limiting the axial travel of the tip on the mounting shaft 32. This insures uniform depth of mounting shaft penetration into the pipette tip to maintain uniform tip interference with the mounting shaft as successive tips are mounted on and ejected from the mounting shaft. In the embodiment illustrated in Fig. 2, such cooperative means 52 comprises an annular, upwardly facing, inwardly directed shoulder 53 on the inner surface of the pipette tip 40 immediately adjacent the lateral support region 48. The shoulder 53 is designed such that an upper surface thereof engages a downwardly facing surface such as the bottom 54 of the distal end 34 of the mounting shaft 32 at an outer circumferential portion thereof.
An alternate embodiment of the cooperative means 52 is depicted in Fig. 4 as comprising an outwardly directed downwardly facing annular shoulder 53' on the pipette tip mounting shaft 32 which upon insertion of the shaft into the open proximal end 42 of the tip engages the upper annular edge 56 of the tip to halt further penetration of the shaft into the tip.

Claims

An air displacement pipette (10) comprising:
a pipette tip mounting shaft (32) in the form of an axially elongate body and a pipette tip (40) in the form of an elongated plastic tube and having an open proximal end (42) mountable onto a distal end (34) of the tip mounting shaft (32) and an open distal sample receiving end, the shaft (32) including an annular sealing zone (36) and a lateral support zone (38) axially spaced from the sealing zone (36) and the tip (40) including an annular sealing region (46) mating with the annular sealing zone (36) of the shaft (32) and a lateral support region (48) axially spaced from the annular sealing region (46) and cooperating with the lateral support zone (38) of the shaft (32), the annular sealing region (46) being adapted to form an interference fit and air tight seal with the sealing zone (36) when the sealing zone penetrates the sealing region, there being means (52) for insuring uniform depth of mounting shaft penetration into the pipette tip to maintain a uniform tip interference with the mounting shaft as successive tips are mounted on and ejected from the mounting shaft, characterized by:
(i) the annular sealing region (46) of the tip (40) and the annular sealing zone (36) of the shaft (32) having a frusto-conical shape, with the outer surface of the frusto-conical distal end of the shaft being substantially parallel to the inner frusto-conical surface of the proximal end portion (42) of the tip (40),

(ii) the sealing region (46) of the tip (40) being disposed adjacent the open proximal end (42) of the tip (40) and nearer to said open proximal end than is said lateral support region (48),

(iii) said sealing region being formed by an inwardly extending bead (49) extending radially inward from the sidewall (50) of the pipette tip to form a narrow inner sealing band or line, the outer diameter of said annular sealing zone (36) of the shaft (32) being slightly greater than the inner diameter of the annular sealing region (46) of the tip and the sidewall of the tip in the annular sealing region (46) being made sufficiently thin that the sealing region (46) expands slightly to form an interference fit and said airtight seal

(iv) the lateral support region (48) of the tip (40) having a frusto-conical inner surface that cooperates with a frusto-conical support zone (38) on the shaft (32), with the outer diameters of the shaft (32) in the axially tapering lateral support zone (38) being slightly less than or substantially equal to corresponding inner diameters of the proximal end portion of the tip (40) in at least some circumferential portions of the axially tapering lateral support region (48) whereby to provide lateral support for the tip (40) that prevents transverse rocking of the tip (40) on the shaft (32) as might otherwise occur during touching off of the pipette tip (40) on the mounting shaft (32), without creating an air-tight seal between the tip (40) and the shaft (32).
The pipette of claim 1 and further characterized by axial spacing of the sealing zone (36) and the support zone (38) that is substantially equal to the axial spacing of the sealing region (46) and the support region (48).
The pipette of claim 1 or claim 2 and further characterized by the sidewall (50) having a thickness of between 0.2 and 0.5 mm.
The pipette of claim 3 and further characterized by the sealing region (46) having an inner diameter which is about 0.075 mm to about 0.2 mm less than corresponding outer diameters of the sealing zone (36).
The pipette of any one of the claims 1 to 4 and further characterized by the sidewalls of the pipette tip in the sealing region (46) and in the lateral support region (48) having a thickness of between 0.2 mm and 0.5 mm, by the sealing region having an inner diameter which is about 0.075 mm to about 0.2 mm less than corresponding outer diameter of the sealing zone (36) and by the support region having an inner diameter which is less than the outer diameter of the lateral support zone (38) by 0.075 mm or less.
The pipette of claim 5 and further characterized by the shaft (32) and the tip being concentric.
The pipette of any one of the preceding claims and further characterized by the pipette tip (40) having an inwardly directed shoulder (53) between the proximal end (42) and the distal end (44) and wherein the sealing region (46) is further removed from the shoulder (53) than the support region (48).
The pipette of any one of the preceding claims and further characterized by similar frusto-conical distal and proximal end portions on the pipette tip (40) mounting shaft (32) and pipette tip (40) respectively with substantially inwardly and downwardly tapering surfaces with a taper between one and a half degrees and six degrees from the longitudinal axis of the shaft (32).