EP1135210A1 - Pipette with improved pipette tip and mounting shaft combination - Google Patents
Pipette with improved pipette tip and mounting shaft combinationInfo
- Publication number
- EP1135210A1 EP1135210A1 EP99971738A EP99971738A EP1135210A1 EP 1135210 A1 EP1135210 A1 EP 1135210A1 EP 99971738 A EP99971738 A EP 99971738A EP 99971738 A EP99971738 A EP 99971738A EP 1135210 A1 EP1135210 A1 EP 1135210A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- pipette tip
- mounting shaft
- sealing
- region
- annular
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/0275—Interchangeable or disposable dispensing tips
- B01L3/0279—Interchangeable or disposable dispensing tips co-operating with positive ejection means
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0689—Sealing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/12—Specific details about materials
- B01L2300/123—Flexible; Elastomeric
Definitions
- the present invention relates to improvements in pipettes and, more particularly, to air displacement pipettes including a unique pipette tip tailored to the distal end of the pipette's tip mounting shaft such that the tip is easily insertable by a pipette user onto the mounting shaft to a fluid tight position in which the tip is secured against undesired lateral rocking on or displacement from the shaft and, after use, is easily ejectable from the shaft by the pipette user.
- the present invention incorporates in an air displacement pipette the concept of similar frusto-conical distal and proximal end portions on a pipette tip mounting shaft and pipette tip respectively.
- the frusto-conical distal and proximal end portions have substantially parallel inwardly and downwardly tapering surfaces incorporating axially spaced annular sealing and lateral support zones and regions on the mounting shaft and tip, respectively.
- the present invention incorporates cooperative means on the shaft and tip for insuring uniform depth of mounting shaft penetration into the pipette tip to maintain uniform tip interference with the mounting shaft as successive tips are mounted on and ejected from the mounting shaft.
- the present invention comprises a combination of a pipette tip mounting shaft and pipette tip in an air displacement pipette.
- the mounting shaft comprises an axially elongated body including a frusto-conical distal end portion having an axially tapering outer surface with annular axially spaced outer surface regions defining an annular sealing zone and an axially tapering annular lateral support zone.
- the pipette tip is an elongated tube comprising an open frusto-conical proximal end portion, an open conical distal end and annular axially spaced inner surface regions on the axially tapering frusto-conical inner surface of the proximal end portion defining an annular sealing region and an axially tapering annular lateral support region.
- the axial tapering of the mounting shaft and pipette tip are one and one-half degrees or more from the longitudinal axis of the shaft and preferably between one and one-half and five or six degrees.
- the outer diameter of the annular sealing zone on the mounting shaft is slightly greater than the inner diameter of the annular sealing region on the pipette tip and the sidewall of the tip in the area of the annular sealing region is sufficiently thin that the annular sealing region expands slightly to form an interference fit and air tight seal between the mounting shaft and the pipette tip when the sealing zone penetrates the sealing region.
- the axial spacing of the sealing and support zones is substantially equal to the axial spacing of the sealing and support regions.
- the outer diameters of the mounting shaft in the axially tapering lateral support zone are slightly less than or substantially equal to corresponding inner diameters of the proximal end portion of the tip in at least some circumferential portions of the axially tapering lateral support region such that as the sealing zone penetrates the sealing region.
- the support region receives the support zone and provides lateral support therefor which prevents transverse rocking of the pipette tip on the mounting shaft as might otherwise occur during touching off of the pipette tip and an accompanying undesired dislodging of the tip from the shaft.
- the preferred embodiment of the present invention includes the aforementioned controlled interference air tight fit and mating annular lateral support zone and region as well as cooperative means on the pipette and pipette tip for limiting the axial travel of the tip on the mounting shaft. This insures uniform depth of mounting shaft penetration into the pipette tip to maintain uniform the desired tip interference with the mounting shaft as successive tips are mounted on and ejected from the mounting shaft.
- the interference fit between the sealing zone and region is about .075 mm to about .2 mm and the wall thickness of the pipette tip in the sealing region is between .2 and .5 mm
- the desired minimal tip mounting and ejection forces associated with the present invention still may be achieved and the lateral stability of the tip on the shaft further enhanced when there is a small interference fit between the support region and zone.
- the small interference is provided by the lateral support region of the tip having an inner diameter which is slightly less than the outer diameter of the lateral support zone of the shaft, eg. less than .075 mm.
- Fig. 1 is a side view of a standard manual pipette having a pipette tip mounted on a mounting shaft adjacent a lower end of a tip ejector mechanism of the pipette.
- Fig. 2 is a cross sectional side view of one embodiment of the pipette tip and mounting shaft combination of the present invention showing the fluid tight seal between the sealing region and sealing zone, the mating relationship of the lateral support region and zone and a preferred embodiment of the cooperative means including a shoulder on the pipette tip for limiting mounting shaft penetration into the tip.
- Fig. 3 is an enlarged fragmentary section side view of the sealing region within the circle 3 for the pipette tip of Fig. 2.
- Fig. 4 is an enlarged fragmentary side view of an upper portion of the pipette tip and mounting shaft combination similar to Fig. 2 showing a first alternative embodiment of the cooperative means including a shoulder on the mounting shaft for limiting mounting shaft penetration into the tip.
- Fig. 5 resembles Fig. 2 and shows an embodiment where the axial location of the sealing zone and region is reversed relative to the axial location of the support zone and region shown in Fig. 2.
- Fig. 6 resembles Fig. 4 and shows an embodiment where the axial location of the sealing zone and region is reversed relative to the axial location of the support zone and region shown in Fig. 4.
- Fig. 7 resembles Fig. 2 and is a cross sectional side view of an embodiment of the pipette tip and mounting shaft combination of the present invention showing the fluid tight seal formed by an interference fit between the sealing region and sealing zone, a small interference fit between the lateral support region and zone and a preferred embodiment of the cooperative means including a shoulder on the pipette tip for limiting mounting shaft penetration into the tip.
- Fig. 8 is an enlarged fragmentary side view of an upper portion of the pipette tip and mounting shaft combination similar to Fig. 7 showing a first alternative embodiment of the cooperative means including a shoulder on the mounting shaft for limiting mounting shaft penetration into the tip.
- Fig. 9 resembles Fig. 7 and shows an embodiment where the axial location of the sealing zone and region is reversed relative to the axial location of the support zone and region shown in Fig. 7.
- Fig. 10 resembles Fig. 8 and shows an embodiment where the axial location of the sealing zone and region is reversed relative to the axial location of the support zone and region shown in Fig. 8.
- Fig. 1 illustrates a standard manual pipette resembling the
- PIPETMAN pipette sold exclusively in the United States by the Rainin Instrument Co. Inc., assignee of the present invention.
- the manual pipette is designated in Fig. 1 by the number 10 and includes a pipette tip ejector mechanism 12 described in United States patent 3,991,617 issued November 16, 1976, which is incorporated herein by this reference.
- the pipette 10 comprises a push button 14 connected by a rod 16 to a piston (not shown) located in the body or housing 18 of the pipette.
- the push button 14 may be depressed by a user exerting a downward force on the push button to cause downward movement of the piston of the pipette.
- a quantity of liquid to be sampled is sucked into a disposable pipette tip 20 releasably secured to a lower end of a pipette tip mounting shaft 22 of the pipette.
- the sample then may be transferred into another vessel by once more exerting a downward force on the push button 14.
- the pipette tip mechanism ejector 12 is employed to eject the tip 20 from the mounting shaft 22.
- the mechanism 12 comprises a push button 24 connected to a rod located in a passage (not shown) provided in an upper part of the hand holdable housing 18 of the pipette 10.
- the passage and rod are arranged so as to be able to impart to the rod a movement of translation parallel to an axis of the pipette in opposition to a spring (not shown) normally urging the rod in an upward position.
- a removable tip ejector member or arm 26 including a tubular upper end extends from a lower end of the rod and from the rod follows the general exterior contour of the housing 18 of the pipette to terminate in a sleeve 28.
- the sleeve 28 encircles a conical lower end 30 of the pipette tip mounting shaft 22 which tightly receives the upper end of the disposable pipette tip 20.
- a user grips the pipette housing 18 and using his or her thumb presses downward on the push button 24.
- the downward force on the push button is translated by the rod to the tip ejector arm 26 and hence to the sleeve 28 which presses down on an upper end of the pipette tip.
- the pipette tip is propelled from the mounting shaft.
- the spring Upon a release of the push button 24, the spring returns the tip ejector mechanism 12 to its normal position with the sleeve spaced slightly from the upper end of a replacement pipette tip which is inserted onto the mounting shaft 22 readying the pipette 10 for its next aspiration and dispensing operation.
- the mounting shaft 32 comprises an axially elongated body including a frusto-conical distal end portion 34 having an outer surface which tapers axially inwardly from a main or upper portion of the shaft.
- the axially tapering outer surface of the distal end portion 34 comprises annular axially spaced outer surface regions defining an annular sealing zone 36 adjacent an upper end of the distal end 34 and an annular axially and downwardly and inwardly tapering lateral support zone 38 on the distal end portion 34 near the lower end of the mounting shaft 32.
- the pipette tip is represented by the numeral 40 and is an elongated plastic tube comprising an open frusto-conical proximal end portion 42, an open conical distal end portion 44 and annular and axially spaced inner surface regions on the axially downwardly and inwardly tapering inner surface 43 of the proximal end portion 42 defining an annular sealing region 46 and an axially tapering annular lateral support region 48 for mating with the sealing and support zones 36 and 38 respectively, on the mounting shaft 32.
- the frusto-conical inner surface of the proximal end portion 42 of the tip 40 is similar to and slightly larger than the frusto-conical outer surface of the distal end portion 34 of the shaft 32.
- the outer surface of the frusto-conical distal end portion of the shaft 32 is substantially parallel to the inner frusto-conical surface of the proximal end portion 42 of the tip 40.
- substantially parallel means that the outer surface of the axially tapering outer surface of the distal end portion 34 is within one and one-half degrees of the axial taper of the inner surface 43 of the proximal end portion 42 of the tip 40.
- Fig. 3 illustrates in enlarged detail a preferred embodiment of the sealing region 46 and comprises the portion of the pipette tip 40 of Fig. 2 within the circle 3.
- the sealing region 46 is formed by an inwardly extending substantially V- shaped bead 49 extending radially inward from the sidewall 50 of the pipette tip 40.
- the innermost surface of the bead 49 forms a very narrow annular sealing band or line for engaging the sealing zone 36 of the pipette tip mounting shaft 32 to form the previously described air-tight seal between the tip and mounting shaft.
- the outer diameter of the annular sealing zone 36 is slightly greater than the inner diameter of the annular sealing region 46 on the pipette tip 40 and the sidewall 50 of the tip in the area of the annular sealing region 46 is sufficiently thin that the annular sealing region expands slightly to form an interference fit and air tight seal between the mounting shaft 32 and the pipette tip 40 when the sealing zone 36 penetrates the sealing region 46.
- the desired interference fit is formed when the difference in the outer diameter of the annular sealing zone and the inner diameter of the annular sealing region is at least .075 millimeters (mm) .
- the wall thickness of the pipette tip in the area of the sealing region 46 is preferably between .20 and .50 mm.
- the axial spacing of the sealing zone and region is substantially equal to the axial spacing of the support zone and region.
- the outer diameters of the shaft 32 within the axially tapering lateral support zone 38 are slightly less than or substantially equal to corresponding inner diameters of the proximal end portion within at least some circumferential portions of the axially tapering lateral support region. This allows for some minimal contact between the support zone and region without creating a secondary air tight which would result in an undesired increase in the axial forces required to mount and eject the pipette tip on and from the shaft.
- the support region 48 receives the support zone 38 and provides lateral support therefor which prevents transverse rocking of the pipette tip 40 on the mounting shaft 32 as might otherwise occur during "touching off" of the pipette tip and an accompanying undesired dislodging of the tip from the shaft.
- the axial spacing of the mating lateral support zone 38 and region 48 from the sealing zone and region (36,46) is substantially equal to the inner diameter of the pipette tip 40 in the portion of the support region engaging the support zone.
- the present invention includes cooperative means 52 on the pipette of the present invention and the pipette tip 40 for limiting the axial travel of the tip on the mounting shaft 32. This insures uniform depth of mounting shaft penetration into the pipette tip to maintain uniform tip interference with the mounting shaft as successive tips are mounted on and ejected from the mounting shaft.
- cooperative means 52 comprises an annular, upwardly facing, inwardly directed shoulder 53 on the inner surface of the pipette tip 40 immediately adjacent the lateral support region 48.
- the shoulder 53 is designed such that an upper surface thereof engages a downwardly facing surface such as the bottom 54 of the distal end 34 of the mounting shaft 32 at an outer circumferential portion thereof.
- FIG. 4 An alternate embodiment of the cooperative means 52 is depicted in Fig. 4 as comprising an outwardly directed downwardly facing annular shoulder 53' on the pipette tip mounting shaft 32 which upon insertion of the shaft into the open proximal 42 of the tip engages the upper annular edge 56 of the tip to halt further penetration of the shaft into the tip.
- FIGS. 5 and 6 show alternative embodiments of the combinations illustrated in Figs. 2 and 4 respectively wherein the axial location of the sealing zone 36 and region 38 is reversed relative to the axial location of the support zone 46 and region 48 respectively.
- Figs. 7, 8, 9 and 10 resemble Figs. 2, 4, 5 and 6 respectively and show alternative embodiments of the present invention where there is a small interference fit between the lateral support region 48 and support zone 38 to further enhance the lateral stability of the tip 40 on the shaft 32 without introducing an undesired increase in the axial forces required to mount and eject the tip from the shaft.
- Figs. 7, 8, 9 and 10 resemble Figs. 2, 4, 5 and 6 respectively and show alternative embodiments of the present invention where there is a small interference fit between the lateral support region 48 and support zone 38 to further enhance the lateral stability of the tip 40 on the shaft 32 without introducing an undesired increase in the axial forces required to mount and eject the tip from the shaft.
- the lateral stability of the tip 40 on the shaft 32 can be further enhanced while maintaining the desired minimal tip mounting and ejection forces associated with the present invention when their is a small interference fit between the support region and zone.
- the small interference is provided by the lateral support region 48 of the tip 40 having an inner diameter which is slightly less than the outer diameter of the lateral support zone 38 of the shaft 32, eg. less than .075 mm.
- a secondary air tight seal may be created between the support zone and region without creating an undesired increase in the axial forces required to mount and eject the tip on and from the shaft.
Landscapes
- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Devices For Use In Laboratory Experiments (AREA)
- Sampling And Sample Adjustment (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Mirrors, Picture Frames, Photograph Stands, And Related Fastening Devices (AREA)
Abstract
Description
Claims
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US234195 | 1994-04-28 | ||
US18803298A | 1998-11-06 | 1998-11-06 | |
US188032 | 1998-11-06 | ||
US09/234,195 US6248295B1 (en) | 1998-11-06 | 1999-01-20 | Pipette with improved pipette tip and mounting shaft combination |
PCT/US1999/026210 WO2000027528A1 (en) | 1998-11-06 | 1999-11-05 | Pipette with improved pipette tip and mounting shaft combination |
Publications (3)
Publication Number | Publication Date |
---|---|
EP1135210A1 true EP1135210A1 (en) | 2001-09-26 |
EP1135210A4 EP1135210A4 (en) | 2006-11-02 |
EP1135210B1 EP1135210B1 (en) | 2010-07-21 |
Family
ID=26883655
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP99971738A Expired - Lifetime EP1135210B1 (en) | 1998-11-06 | 1999-11-05 | Pipette with improved pipette tip and mounting shaft combination |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP1135210B1 (en) |
CN (1) | CN1146473C (en) |
BR (1) | BR9915086A (en) |
PL (1) | PL192753B1 (en) |
TW (1) | TW431912B (en) |
WO (1) | WO2000027528A1 (en) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6596240B2 (en) | 2001-01-12 | 2003-07-22 | Porex Corporation | Pipette tip for easy mounting and ejecting from a pipette |
WO2013170538A1 (en) * | 2012-05-16 | 2013-11-21 | Gene Era Biotech Co. Ltd. | Pipette tip member |
CN107398310A (en) * | 2017-02-22 | 2017-11-28 | 佛山市工芯精密机械有限公司 | A kind of pipettor is interrupted |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4023716A (en) * | 1976-04-20 | 1977-05-17 | Justin Joel Shapiro | Micro-dispensing liquid pipet |
US4349109A (en) * | 1980-10-20 | 1982-09-14 | Medical Laboratory Automation, Inc. | Disposable pipette tips and trays therefor |
US4824641A (en) * | 1986-06-20 | 1989-04-25 | Cetus Corporation | Carousel and tip |
US5200151A (en) * | 1990-05-21 | 1993-04-06 | P B Diagnostic Systems, Inc. | Fluid dispensing system having a pipette assembly with preset tip locator |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2287941A1 (en) | 1974-10-15 | 1976-05-14 | Marteau D Autry Eric | DEVICE FOR EJECTING THE REMOVABLE TIP OF A PIPETTE |
US4748859A (en) * | 1987-03-06 | 1988-06-07 | Rainin Instrument Co., Inc. | Disposable pipette tip |
DE3824767A1 (en) * | 1988-07-21 | 1990-02-01 | Eppendorf Geraetebau Netheler | CLIP-ON PIPETTE TIP IN THE FORM OF A CORRESPONDING HEAD PIECE, IN PARTICULAR CONE OF A PIPETTE, AT LEAST IN PARTIAL CONTAINERS |
US5232669A (en) * | 1991-11-08 | 1993-08-03 | Abbott Laboratories | Pipette tip with self-aligning and self-sealing features |
-
1999
- 1999-11-05 WO PCT/US1999/026210 patent/WO2000027528A1/en active IP Right Grant
- 1999-11-05 PL PL366207A patent/PL192753B1/en unknown
- 1999-11-05 BR BR9915086-7A patent/BR9915086A/en not_active Application Discontinuation
- 1999-11-05 EP EP99971738A patent/EP1135210B1/en not_active Expired - Lifetime
- 1999-11-05 CN CNB998143359A patent/CN1146473C/en not_active Expired - Lifetime
-
2000
- 2000-03-01 TW TW88119446A patent/TW431912B/en not_active IP Right Cessation
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4023716A (en) * | 1976-04-20 | 1977-05-17 | Justin Joel Shapiro | Micro-dispensing liquid pipet |
US4349109A (en) * | 1980-10-20 | 1982-09-14 | Medical Laboratory Automation, Inc. | Disposable pipette tips and trays therefor |
US4824641A (en) * | 1986-06-20 | 1989-04-25 | Cetus Corporation | Carousel and tip |
US5200151A (en) * | 1990-05-21 | 1993-04-06 | P B Diagnostic Systems, Inc. | Fluid dispensing system having a pipette assembly with preset tip locator |
Non-Patent Citations (1)
Title |
---|
See also references of WO0027528A1 * |
Also Published As
Publication number | Publication date |
---|---|
PL366207A1 (en) | 2005-01-24 |
CN1146473C (en) | 2004-04-21 |
EP1135210B1 (en) | 2010-07-21 |
WO2000027528A1 (en) | 2000-05-18 |
TW431912B (en) | 2001-05-01 |
EP1135210A4 (en) | 2006-11-02 |
PL192753B1 (en) | 2006-12-29 |
BR9915086A (en) | 2001-07-17 |
CN1348397A (en) | 2002-05-08 |
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