EP1132135B1 - Individual closure and closure carrier for reaction wells, storage and dispensing device for individual closures - Google Patents

Individual closure and closure carrier for reaction wells, storage and dispensing device for individual closures Download PDF

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Publication number
EP1132135B1
EP1132135B1 EP01104986A EP01104986A EP1132135B1 EP 1132135 B1 EP1132135 B1 EP 1132135B1 EP 01104986 A EP01104986 A EP 01104986A EP 01104986 A EP01104986 A EP 01104986A EP 1132135 B1 EP1132135 B1 EP 1132135B1
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EP
European Patent Office
Prior art keywords
closure
individual
carrier
arrangement according
positively locking
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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EP01104986A
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German (de)
French (fr)
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EP1132135A3 (en
EP1132135A2 (en
Inventor
Günter Kohrmann
Antonio Dr. Romaguera
Peter Mahler
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Brand GmbH and Co KG
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Brand GmbH and Co KG
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50853Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids

Definitions

  • the invention relates to an arrangement for contamination-free processing in particular molecular biological reaction sequences with the Features of the preamble of claim 1.
  • Subject of the invention also a corresponding closure carrier for such an arrangement, a single closure for such an arrangement and a storage and dispensing arrangement for such individual closures.
  • nucleic acid diagnostics A particularly problematic area is nucleic acid diagnostics, in which The prevention of contamination is very important.
  • special Importance of modern duplication methods such as the polymerase chain reaction, through which the detection limit significantly reduced becomes.
  • the danger of falsification of the measurement results increases through the previously mentioned contaminations.
  • nucleic acid diagnostics may total be referred to the EP 0 676 643 A2, from the extensive See further versions.
  • the individual closures are here on the closure carrier by held a one-piece or multi-part adhesive film and can so together with the closure carrier all sealing together on the reaction vessels put on, but also together or in groups, together with the closure carrier be deducted from these again. If you remove the adhesive film, Thus, the respective individual closure remains sealingly on the reaction vessel.
  • the individual closures are in the previously described prior art as executed comprehensive cover, so that the respective closure portion the open end of the reaction vessel bordered edge.
  • the Reaction vessels according to the connection plane of the reaction vessels have protruding edge above. It is therefore in the arrangement with the reaction vessels in this prior art is not a typical Microtiter plate, which is indeed executed plan at the top.
  • the starting point for teaching forming arrangement to contamination-free processing of particular molecular biological Reaction processes is a problem in that the selective Opening a single reaction vessel, first the adhesive film for all Reaction vessels are removed and then removed the closure carrier must be, if one wants to ensure that to avoid Contaminations all other reaction vessels remain closed.
  • the Teaching therefore sets itself the goal of eliminating this problem.
  • the invention moreover, the individual parts of a Arrangement of the type in question, ie in particular the closure carrier and the individual closures.
  • the subject matter of the invention is a supply and dispensing arrangement for individual closures, since it has been shown according to the invention that the Single closures also individually, so without the closure carrier, on the reaction vessels can be used like that, of course, from the EP 0 676 643 A 2, EP 0 734 769 A 1).
  • the teaching of the invention can be particularly useful in context with an industrial robotic system or for such an industrial robotic system use.
  • microtiter plate shown in FIG. 1 for 96 reaction vessels for small and smallest amounts of sample liquid is an example of an arrangement interconnected reaction vessels, not limiting to understand is. Also strip-like arrangements of several together connected reaction vessels are the subject of the invention.
  • PCR technology As has been extensively explained in the prior art (see, for example, EP 0 676 643 A2).
  • the capacity of a reaction vessel 2 is enclosed this application example at about 200 to 400 ul. That too is not restrictive to understand.
  • Fig. 2 shows that in the inventive arrangement each reaction vessel 2 is associated with a single closure 3.
  • Fig. 4 shows such a single closure 3 in a side view. It can be seen that the single closure 3 a closure portion 4 for sealing the opening of the reaction vessel 2 and an actuating portion 5 for engaging the single closure 3 for the purpose of managing it.
  • FIGS. 2 and 3 further show an all reaction vessels 2 of the microtiter plate 1 covering closure carrier 6, each with a receptacle 7 for the respective Operating section 5 of each single closure 3 for securing the single closure 3 on the closure carrier 6.
  • the closure carrier 6 can only cover a group of reaction vessels 2 of the overall arrangement, so that the overall arrangement then has a plurality of closure carrier 6.
  • Figs. 2, 3 and 4 can be seen in connection now that in the teaching of Invention is provided that the closure portions 4 of the individual closures 3 are plugged through the receptacles 7 in the closure carrier 6 therethrough, So when inserting and pulling out of the closure carrier 6 not collide with the closure carrier 6.
  • the closure carrier 6 is an independent "lid carrier” the individual closures 3 are so releasably attached to them that they too individually taken out in fitting to the reaction vessel 2 closure carrier 6 can be. This allows the single opening of each reaction vessel 2 with otherwise closed other reaction vessels. 2 This has the technical handling explained in the general part of the description Advantages and in particular avoids any cross-contamination.
  • the illustrated and In this respect preferred embodiment shows that the individual closures 3 on the closure carrier 6 by means of a releasable positive connection 8, 9 are fixed between the operating section 5 and receptacle 7. This can be seen particularly well in a Zulsanunenschau of Fig. 2, 3 and 4. Die lateral dimensions of the receptacle 7 and the attachment portion. 5 of the single closure 3 are in the illustrated and preferred embodiment chosen and the positive connection 8, 9 is designed so that they are inserted into the receptacle 7 single closure 3 a slight lateral displacement of the single closure 3 in the receptacle 7 allowed.
  • a normal bayonet lock reaches its closed position by a Rotary movement in one direction and its opening position by a Rotary motion in the opposite direction.
  • Such a bayonet lock can also be used in the invention for the positive connection 8, 9 become.
  • the preferred embodiment shows a construction in which the bayonet lock in both directions can be actuated is and has no stop, but a surmountable detent 8a. It can be seen the detent 8a on each pin part 8 in Fig. 5. With this construction the operator is free, in which direction they the bayonet lock wants to operate, it always reaches the other functional position of the bayonet lock.
  • Fig. 2 indicates and Fig. 5 also shows the single closure 3 more clearly that the single closure 3, in particular and shown here on the operating section 5, a closure handling means 10 for attacking one of the handling a single single closure 3 serving operating tool 11 has.
  • the actuating tool 11 indicated as a pin-like hand tool. But one is for more extensive Handling often also automatically moved and operated Insert actuation tools 11, how to stand out from the stand Technique is known.
  • the closure handling means 10 is designed as a form-locking element, to which a corresponding Positive locking element 12 corresponds to the actuating tool 11. More specifically, in the illustrated embodiment, the shutter handling means 10 as a part, namely here as a sliding part, a bayonet closure executed.
  • the form-locking element 12 on the actuating tool 11 is as the other part, in particular the pin part of the bayonet closure executed.
  • closure handling means 10 and the corresponding Positive locking element 12 shows the illustrated embodiment a standard, classic bayonet closure construction. It should be so far expediently provided that after production of the bayonet closure engagement a rotation of the individual closure used in the receptacle 7 3 is effectively possible in both directions, so that the present invention realized in the preferred embodiment Right / left insensitivity of the positive connection 8, 9 also exploited can be.
  • the illustrated and preferred embodiment shows in particular FIGS. 4 and 5, that the single closure 3, the positive connection 8, 9 or frictional connection is arranged on the outside of the actuating section 5, while the closure handling means 10 of the single closure 3 inside is arranged.
  • closure portion 4 of a single closure 3 With regard to the interaction of the closure portion 4 of a single closure 3 with the open end of the associated reaction vessel. 2 can, as in the prior art, be provided that the closure portion 4 of the single closure 3, the reaction vessel 2 overlapped.
  • the illustrated and preferred embodiment shows an embodiment which characterized in that the closure portion 4 of the single closure 3 enters the open end of the reaction vessel 2 like a plug.
  • This is known per se from the prior art. That allows one particularly expedient, realized here in the embodiment also Conception in such a way that the fastening portions 5 of the individual closures 3 consist of a relatively hard, rigid plastic material, the Closure sections 4 are integrally formed materially and from a consist of relatively soft, rubber-elastic plastic material.
  • the prior art has been explained that even individual closures. 3 are known, which have a thin membrane through which the interior of the each reaction vessel can be reached with a needle.
  • the illustrated and preferred embodiment shows a similar construction, namely a construction in which the closure portion 4 is a pierceable Membrane section 4a has. This one is through a central one Passage 5a reachable in the attachment section 5. Both show Fig. 5.
  • the Execution of the closure portion 4 of a relatively soft, rubber-elastic Plastic material, in particular of a thermoplastic elastomer offers the convenient possibility that a puncture in the Diaphragm section 4a of the closure section 4 closes again by itself, because the thermoplastic elastomer has a sufficient restoring force.
  • Fig. 6 shows an enlarged view
  • the operating tool 11 with the therein form-locking element 12 and a possibly spring-loaded Thrust pin 13 the fixation of the bayonet lock for Closure handling means 10 of the single closure 3 makes.
  • the spring force for fixing the Bayonet closure by the inherent elasticity of the closure portion. 4 is applied. In the illustrated embodiment is to the Thrust pin 13 in the passage 5a directly on the material of the closure portion 4, so that this additional function is performed.
  • Fig. 3 shows the closure carrier 6 of an inventive arrangement for 2 shows the closure carrier 6 equipped with individual closures 3.
  • Such closure carrier are also self-negotiable elements, the therefore be subject to independent protection. The same applies to the Single closures 3.
  • Fig. 7 shows schematically an example of a supply and dispensing arrangement 14 for individual closures 3 of the type in question.
  • This is equipped with its own form-locking formation 15, with the form-locking education, namely the pin parts 8, on the operating section. 5 the single closure 3 cooperates in such a way that the individual closures 3 in exactly predetermined, certain lateral orientation of the Supply and dispensing arrangement 14 can be dispensed. So you can specifically a Equipping either the closure carrier 6 with the individual closures. 3 accomplish or even directly equipping the reaction vessels. 2 without closure carrier 6.
  • the arrangement according to the invention can be particularly useful within of an industrial robot system, with the reaction sequences of in Speech type in series and essentially automatically checked become.
  • the operating tool 11th Be part of the industrial robot system for example on an actuating arm arranged, whereby the industrial robot system also the reaction vessels 2 and the closure carrier 3 automatically handles, at least operable fixed.
  • the teaching is also a use of an arrangement of in Talking style in an industrial robot system.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Analytical Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Closures For Containers (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Devices For Use In Laboratory Experiments (AREA)

Abstract

Microbial reactor array for preventing contamination comprising removable plugs fastened in carrier over-reactors, is new. Closure sections of individual plugs (3) can pass through the carrier (6) in both directions. Individual plugs are so fastened to the carrier that with the carrier set down on the reaction vessels (2), each individual plug can be removed separately from the carrier and from the reaction vessel. An Independent claim is also included for a storage and dispensing unit for individual plugs which facilitates contamination-free processing.

Description

Gegenstand der Erfindung ist eine Anordnung zu kontaminationsfreier Bearbeitung von insbesondere molekularbiologischen Reaktionsabläufen mit den Merkmalen des Oberbegriffs von Anspruch 1. Gegenstand der Erfindung ist auch ein entsprechender Verschlußträger für eine solche Anordnung, ein Einzelverschluß für eine solche Anordnung und eine Vorrats- und Abgabeanordnung für derartige Einzelverschlüsse.The invention relates to an arrangement for contamination-free processing in particular molecular biological reaction sequences with the Features of the preamble of claim 1. Subject of the invention also a corresponding closure carrier for such an arrangement, a single closure for such an arrangement and a storage and dispensing arrangement for such individual closures.

Anordnungen zu möglichst kontaminationsfreier Bearbeitung von Reaktionsabläufen sind seit längerem insbesondere für Reihenuntersuchungen in der Molekularbiologie sowie auf angrenzenden Gebieten der Analytik, beispielsweise im Gesundheitswesen, im Bereich der Lebensmittelchemie und der Umweltchemie sehr gefragt. Dabei ist die Kontamination, insbesondere die Kreuz-Kontamination bei der Handhabung der Reaktionsgefäße ein erhebliches Problem. Insbesondere bei besonders niedrig konzentrierten und/oder wenig modifizierten Reaktionsproben wirken sich Verunreinigungen stark auf das Analyseergebnis aus.Arrangements for contamination-free processing of reaction sequences have been for a long time especially for screening in Molecular biology and adjacent fields of analysis, for example in healthcare, in the field of food chemistry and very much in demand for environmental chemistry. Here is the contamination, in particular the cross-contamination in the handling of the reaction vessels a significant Problem. Especially at particularly low concentration and / or little modified reaction samples are contaminants strong on the analysis result.

Ein besonders problematisches Gebiet ist die Nukleinsäurediagnostik, bei der die Vermeidung von Kontaminationen sehr wichtig ist. Hier kommt besondere Bedeutung modernen Vervielfältigungsverfahren zu, wie beispielsweise der Polymerase Chain Reaction, durch die die Nachweisgrenze deutlich herabgesetzt wird. Dafür steigt aber die Gefahr der Verfälschung der Meßergebnisse durch die zuvor angesprochenen Kontaminationen. Für den Hintergrund von Verfahren insbesondere der Nukleinsäurediagnostik darf insgesamt auf die EP 0 676 643 A2 verwiesen werden, aus der sich umfangreiche weitere Ausführungen entnehmen lassen.A particularly problematic area is nucleic acid diagnostics, in which The prevention of contamination is very important. Here comes special Importance of modern duplication methods, such as the polymerase chain reaction, through which the detection limit significantly reduced becomes. But the danger of falsification of the measurement results increases through the previously mentioned contaminations. For the background of procedures, in particular nucleic acid diagnostics may total be referred to the EP 0 676 643 A2, from the extensive See further versions.

Bearbeitungstechnisch interessant ist es, eine Anordnung aus mehreren miteinander verbundenen, oben offenen Reaktionsgefäßen mit Verschlüssen der Reaktionsgefäße zu verwenden, insbesondere sogenannte Mikrotiterplatten mit entsprechend großflächigen Verschlußdeckeln. Ein solcher manuell aufreißbarer gemeinsamer Verschlußdeckel hat den Nachteil, daß bei der Öffnung der Mikrotiterplatte Spritzer von einem Reaktionsgefäß in ein benachbartes Reaktionsgefäß gelangen können, was ja gerade vermieden werden soll (Stand der Technik in der EP 0 676 643 A2 sowie WO 99/44.745 und, für eine Streifenanordnung, US 5,683,659 A). Bei diesen Anordnungen kommt als Nachteil hinzu, daß die einzelnen Reaktionsgefäße nicht einzeln geöffnet werden können. Das möchte man aber gelegentlich aus analysetechnischen Gründen.It is interesting to process an arrangement of several with each other connected, open-top reaction vessels with closures of To use reaction vessels, in particular so-called microtiter plates with correspondingly large closure caps. Such a manually breakable common closure lid has the disadvantage that at the opening the microtiter plate splashes from one reaction vessel into an adjacent one Reaction vessel can get, which is just to be avoided (Prior art in EP 0 676 643 A2 and WO 99/44745 and, for a strip arrangement, US 5,683,659 A). In these arrangements comes added as a disadvantage that the individual reaction vessels are not opened individually can be. But one would like to do this occasionally from analytic Establish.

Unter Berücksichtigung der zuvor erläuterten Schwierigkeiten ist bereits vorgeschlagen worden, zwar mit mehreren nebeneinander angeordneten und miteinander verbundenen, oben offenen Reaktionsgefäßen, insbesondere nach Art einer Mikrotiterplatte, zu arbeiten, diese jedoch jeweils einzeln durch einen Einzelverschluß zu verschließen, wobei die Einzelverschlüsse dann wiederum automatisch gehandhabt werden können (EP 0 676 643 A2 und EP 0 734 769 A1). Das hat naturgemäß den Nachteil, daß die Handhabung der Vielzahl von Einzelverschlüssen aufwendig ist.Taking into account the above-mentioned difficulties is already been proposed, although with several juxtaposed and interconnected, open-top reaction vessels, in particular in the manner of a microtiter plate to work, but each one individually to close by a single closure, the individual closures then in turn can be handled automatically (EP 0 676 643 A2 and EP 0 734 769 A1). This naturally has the disadvantage that the handling the number of individual closures is expensive.

In allerjüngster·Zeit ist eine Anordnung einer Mehrzahl von Reaktionsgefäßen vorgeschlagen worden (WO 00/02.661), bei der die Reaktionsgefäße mit einer Kunststoff-Trägerplattform als ein Kunststoffteil ausgebildet sind, mit der Trägerplattform aber über Sollbruchstellen verbunden sind. Durch Brechen der Sollbruchstellen lassen sich die einzelnen Reaktionsgefäße vom Träger einmalig trennen, um so einzeln gehandhabt werden zu können. Diese Technik ist nur für wenige diagnostische Verfahren geeignet, weil die Anordnung nach Abtrennen eines Reaktionsgefäßes an der entsprechenden Stelle eben zerstört ist. Ein anderer Vorschlag (EP 0 976 453 A2) versucht Kreuz-Kontaminationen durch Glas-Einsätze mit dauerhaft aufgesteckten Verschlüssen zu vermeiden. Die Verschlüsse sind integraler Bestandteil eines zusammenhängenden Verschlußträgers. Jeder Verschluß weist eine dünne Membran auf, durch die das Innere des Reaktionsgefäßes auch dann mit einer Nadel o. dgl. erreichbar ist, wenn, wie hier vorgeschlagen, die Einzelverschlüsse stets aufgesetzt bleiben. Most recently, an arrangement of a plurality of reaction vessels has been proposed (WO 00 / 02.661), in which the reaction vessels are formed with a plastic carrier platform as a plastic part, but are connected to the carrier platform via predetermined breaking points. By Breaking the predetermined breaking points, the individual reaction vessels from Separate carrier once, so as to be able to be handled individually. These Technique is only suitable for a few diagnostic procedures because of the arrangement after separating a reaction vessel at the appropriate location just destroyed. Another proposal (EP 0 976 453 A2) attempts cross-contaminations through glass inserts with permanently attached closures to avoid. The closures are an integral part of a coherent Bolt carrier. Each closure has a thin membrane through, through which the inside of the reaction vessel with a needle o. The like. Is achievable if, as proposed here, the individual closures always stay on top.

Bei der Anordnung zu kontaminationsfreier Bearbeitung von insbesondere molekularbiologischen Reaktionsabläufen, von der die Erfindung ausgeht (US 4,599,314 A) ist durch Einzelverschlüsse der miteinander verbundenen, insbesondere nach Art einer Mikrotiterplatte angeordneten Reaktionsgefäße die Problematik der Kontamination und Kreuz-Kontamination, die dort erkannt worden ist (Spalte 1, Zeilen 63 bis 66) bereits berücksichtigt worden. Hier ist ein handhabungstechnischer Vorteil dadurch realisiert, daß zusätzlich zu den Einzelverschlüssen ein alle Reaktionsgefäße überdeckender Verschlußträger mit je einer Aufnahme für den Betätigungsabschnitt jedes Einzelverschlusses zum Befestigen des Einzelverschlusses am Verschlußträger vorgesehen ist. Die Einzelverschlüsse werden hier am Verschlußträger durch eine einteilige oder mehrteilige Klebefolie gehalten und können so gemeinsam mit dem Verschlußträger alle zusammen auf die Reaktionsgefäße dichtend aufgesetzt, aber auch gemeinsam oder gruppenweise mitsamt dem Verschlußträger von diesen wieder abgezogen werden. Entfernt man die Klebefolie, so verbleibt der jeweilige Einzelverschluß abdichtend auf dem Reaktionsgefäß.In the arrangement for contamination-free processing of particular molecular biological reactions, from which the invention proceeds (US 4,599,314 A) is interconnected by individual closures, in particular arranged in the manner of a microtiter plate reaction vessels the problem of contamination and cross-contamination that detected there already been considered (column 1, lines 63 to 66). Here is a handling technical advantage realized that in addition to the individual closures a closure carrier covering all reaction vessels each with a receptacle for the operating section of each individual closure for attaching the single closure to the closure carrier is provided. The individual closures are here on the closure carrier by held a one-piece or multi-part adhesive film and can so together with the closure carrier all sealing together on the reaction vessels put on, but also together or in groups, together with the closure carrier be deducted from these again. If you remove the adhesive film, Thus, the respective individual closure remains sealingly on the reaction vessel.

Die Einzelverschlüsse sind bei dem zuvor erläuterten Stand der Technik als überfassende Deckel ausgeführt, also so, daß der jeweilige Verschlußabschnitt das offene Ende des Reaktionsgefäßes randseitig überfaßt. Dazu müssen die Reaktionsgefäße einen von der Verbindungsebene der Reaktionsgefäße nach oben abragenden Rand aufweisen. Es handelt sich also bei der Anordnung mit den Reaktionsgefäßen bei diesem Stand der Technik nicht um eine typische Mikrotiterplatte, die ja an der Oberseite plan ausgeführt ist.The individual closures are in the previously described prior art as executed comprehensive cover, so that the respective closure portion the open end of the reaction vessel bordered edge. To do this, the Reaction vessels according to the connection plane of the reaction vessels have protruding edge above. It is therefore in the arrangement with the reaction vessels in this prior art is not a typical Microtiter plate, which is indeed executed plan at the top.

Bei der zuvor erläuterten, den Ausgangspunkt für die Lehre bildenden Anordnung zu kontaminationsfreier Bearbeitung von insbesondere molekularbiologischen Reaktionsabläufen besteht ein Problem darin, daß zur selektiven Öffnung eines einzelnen Reaktionsgefäßes zunächst die Klebefolie für alle Reaktionsgefäße entfernt werden und dann der Verschlußträger abgenommen werden muß, wenn man gewährleisten will, daß zur Vermeidung von Kontaminationen alle anderen Reaktionsgefäße geschlossen bleiben. Die Lehre setzt sich daher zum Ziel, dieses Problem zu eliminieren. In the previously explained, the starting point for teaching forming arrangement to contamination-free processing of particular molecular biological Reaction processes is a problem in that the selective Opening a single reaction vessel, first the adhesive film for all Reaction vessels are removed and then removed the closure carrier must be, if one wants to ensure that to avoid Contaminations all other reaction vessels remain closed. The Teaching therefore sets itself the goal of eliminating this problem.

Das zuvor aufgezeigte Problem ist bei der Anordnung nach der Lehre der vorliegenden Erfindung mit den Merkmalen des kennzeichnenden Teils von Anspruch 1 gelöst. Wesentlich ist die Erkenntnis, daß die Abmessungen der Verschlußabschnitte der Einzelverschlüsse einerseits und der Aufnahmen im Verschlußträger andererseits so aufeinander abgestimmt sein sollten, daß die Verschlußabschnitte durch die Aufnahmen hindurch steckbar und damit auch wieder entfernbar sind. Wenn man dann die Einzelverschlüsse am Verschlußträger so befestigt, daß die Einzelverschlüsse auch bei auf die Reaktionsgefäße aufgesetztem Verschlußträger vom Verschlußträger einzeln gelöst werden können, so kann jedes Reaktionsgefäß bei der erfindungsgemäßen Anordnung beliebig einzeln geöffnet oder später wieder verschlossen werden. Diese Handhabungstechnik trägt den Anforderungen insbesondere der Nukleinsäurediagnostik in hervorragendem Maße Rechnung und vermeidet wirklich sicher und ohne besonderen Aufwand alle Kontaminationen und Kreuzkontaminationen.The problem indicated above is in the arrangement according to the teaching of present invention with the features of the characterizing part of Claim 1 solved. It is essential to realize that the dimensions of Closure sections of the individual closures on the one hand and the recordings in the On the other hand closure carrier should be coordinated so that the Closure sections plugged through the receptacles and thus also are removable again. If you then the individual closures on the closure carrier fixed so that the individual closures also on the reaction vessels attached closure carrier are detached from the closure carrier individually can, then each reaction vessel in the inventive arrangement can be opened individually or closed again later. These Handling technology fulfills the requirements of nucleic acid diagnostics in particular to a great extent and really avoids safe and without any special effort all contaminations and cross contamination.

Bevorzugte Ausgestaltungen und Weiterbildungen der zuvor erläuterten Lehre sind Gegenstand der Unteransprüche.Preferred embodiments and developments of the previously explained Teaching are the subject of the subclaims.

Gegenstand der Erfindung sind im übrigen auch die einzelnen Teile einer Anordnung der in Rede stehenden Art, also insbesondere der Verschlußträger und die Einzelverschlüsse.The invention, moreover, the individual parts of a Arrangement of the type in question, ie in particular the closure carrier and the individual closures.

Gegenstand der Erfindung ist schließlich auch eine Vorrats- und Abgabeanordnung für Einzelverschlüsse, da sich erfindungsgemäß gezeigt hat, daß die Einzelverschlüsse auch einzeln, also ohne den Verschlußträger, auf den Reaktionsgefäßen verwendet werden können wie das natürlich an sich aus dem eingangs erläuterten Stand der Technik bekannt ist (EP 0 676 643 A 2, EP 0 734 769 A 1).Finally, the subject matter of the invention is a supply and dispensing arrangement for individual closures, since it has been shown according to the invention that the Single closures also individually, so without the closure carrier, on the reaction vessels can be used like that, of course, from the EP 0 676 643 A 2, EP 0 734 769 A 1).

Die Lehre der Erfindung läßt sich besonders zweckmäßig im Zusammenhang mit einem Industrie-Robotersystem einsetzen oder für ein solches Industrie-Robotersystem verwenden. The teaching of the invention can be particularly useful in context with an industrial robotic system or for such an industrial robotic system use.

Im folgenden wird nun die Erfindung anhand einer lediglich ein Ausführungsbeispiel darstellenden Zeichnung näher erläutert. In der Zeichnung zeigt

Fig. 1
in perspektivischer Ansicht schematisch zunächst eine Anordnung von miteinander verbundenen, oben offenen Reaktionsgefäßen, hier in Form einer Mikrotiterplatte mit 96 Reaktionsgefäßen,
Fig. 2
eine komplette erfindungsgemäße Anordnung in einem Ausführungsbeispiel,
Fig. 3
einen Verschlußträger einer erfindungsgemäßen Anordnung gemäß Fig. 2,
Fig. 4
ein Ausführungsbeispiel eines bei der Anordnung aus Fig. 2 verwendbaren Einzelverschlusses in einer Seitenansicht,
Fig. 5
den Einzelverschluß aus Fig. 4 in einer perspektivischen Ansicht von oben,
Fig. 6
eine vergrößerte Seitenansicht eines Betätigungswerkzeugs zur Handhabung eines Einzelverschlusses,
Fig. 7
in schematischer Darstellung eine Vorrats- und Abgabeanordnung für Einzelverschlüsse gemäß der Erfindung.
In the following the invention will now be explained in more detail with reference to a drawing showing only one embodiment. In the drawing shows
Fig. 1
in a perspective view schematically first an arrangement of interconnected, open-topped reaction vessels, here in the form of a microtiter plate with 96 reaction vessels,
Fig. 2
a complete arrangement according to the invention in one embodiment,
Fig. 3
a closure carrier of an inventive arrangement according to FIG. 2,
Fig. 4
An embodiment of a usable in the arrangement of FIG. 2 single closure in a side view,
Fig. 5
4 shows the single closure from FIG. 4 in a perspective view from above,
Fig. 6
an enlarged side view of an actuating tool for handling a single closure,
Fig. 7
a schematic representation of a supply and dispensing arrangement for individual closures according to the invention.

Die in Fig. 1 dargestellte Mikrotiterplatte für 96 Reaktionsgefäße für kleine und kleinste Mengen von Probenflüssigkeit ist ein Beispiel für eine Anordnung miteinander verbundener Reaktionsgefäße, das nicht einschränkend zu verstehen ist. Auch streifenförmige Anordnungen von mehreren miteinander verbundenen Reaktionsgefäßen sind Gegenstand der Erfindung.The microtiter plate shown in FIG. 1 for 96 reaction vessels for small and smallest amounts of sample liquid is an example of an arrangement interconnected reaction vessels, not limiting to understand is. Also strip-like arrangements of several together connected reaction vessels are the subject of the invention.

Die in Fig. 1 perspektivisch gezeigte Mikrotiterplatte 1 als Anordnung von kleinen, miteinander verbundenen, nach oben offenen Reaktionsgefäßen 2 eignet sich beispielsweise gut für die Durchführung der PCR-Technologie wie im Stand der Technik umfangreich erläutert worden ist (siehe z. B. die EP 0 676 643 A2). Das Fassungsvermögen eines Reaktionsgefäßes 2 liegt bei diesem Anwendungsbeispiel bei etwa 200 bis 400 µl. Auch das ist aber nicht einschränkend zu verstehen.The microtiter plate 1 shown in perspective in FIG. 1 as an arrangement of small, interconnected, open-topped reaction vessels 2 For example, it is well suited for the implementation of PCR technology as has been extensively explained in the prior art (see, for example, EP 0 676 643 A2). The capacity of a reaction vessel 2 is enclosed this application example at about 200 to 400 ul. That too is not restrictive to understand.

Fig. 2 zeigt, daß bei der erfindungsgemäßen Anordnung jedem Reaktionsgefäß 2 ein Einzelverschluß 3 zugeordnet ist. Fig. 4 zeigt einen solchen Einzelverschluß 3 in einer Seitenansicht. Man erkennt, daß der Einzelverschluß 3 einen Verschlußabschnitt 4 zum dichten Verschließen der Öffnung des Reaktionsgefäßes 2 sowie einen Betätigungsabschnitt 5 zum Angreifen am Einzelverschluß 3 zum Zwecke seines Handhabens aufweist.Fig. 2 shows that in the inventive arrangement each reaction vessel 2 is associated with a single closure 3. Fig. 4 shows such a single closure 3 in a side view. It can be seen that the single closure 3 a closure portion 4 for sealing the opening of the reaction vessel 2 and an actuating portion 5 for engaging the single closure 3 for the purpose of managing it.

Fig. 2 und 3 zeigen ferner einen alle Reaktionsgefäße 2 der Mikrotiterplatte 1 überdeckenden Verschlußträger 6 mit je einer Aufnahme 7 für den jeweiligen Betätigungsabschnitt 5 jedes Einzelverschlusses 3 zum Befestigen des Einzelverschlusses 3 am Verschlußträger 6. Der Verschlußträger 6 kann auch nur eine Gruppe von Reaktionsgefäßen 2 der Gesamtanordnung überdecken, so daß die Gesamtanordnung dann mehrere Verschlußträger 6 aufweist.FIGS. 2 and 3 further show an all reaction vessels 2 of the microtiter plate 1 covering closure carrier 6, each with a receptacle 7 for the respective Operating section 5 of each single closure 3 for securing the single closure 3 on the closure carrier 6. The closure carrier 6 can only cover a group of reaction vessels 2 of the overall arrangement, so that the overall arrangement then has a plurality of closure carrier 6.

Die am Verschlußträger 6 befestigten Einzelverschlüsse 3, das zeigt Fig. 2, sind gemeinsam mit dem Verschlußträger 6 auf die Reaktionsgefäße 2, also die Mikrotiterplatte 1, dichtend aufsetzbar, aber auch gemeinsam mit dem Verschlußträger 6 von diesen wieder abziehbar.The fastened to the closure carrier 6 individual closures 3, which shows Fig. 2, are together with the closure carrier 6 on the reaction vessels 2, so the Microtiter plate 1, sealingly attachable, but also together with the closure carrier 6 of these again deductible.

Fig. 2, 3 und 4 lassen im Zusammenhang nun erkennen, daß bei der Lehre der Erfindung vorgesehen ist, daß die Verschlußabschnitte 4 der Einzelverschlüsse 3 durch die Aufnahmen 7 im Verschlußträger 6 hindurch steckbar sind, also beim Hineinstecken und Herausziehen aus dem Verschlußträger 6 nicht mit dem Verschlußträger 6 kollidieren. Mit den Betätigungsabschnitten 5 sind die Einzelverschlüsse 3 weiter derart am Verschlußträger 6 befestigt, daß bei auf die Reaktionsgefäße 2 aufgesetztem Verschlußträger 6 mitsamt Einzelverschlüssen 3 jeder Einzelverschluß 3 auch einzeln vom Verschlußträger 6 und vom zugeordneten Reaktionsgefäß 2 abnehmbar ist. Mit anderen Worten ist erfindungsgemäß der Verschlußträger 6 ein eigenständiger "Deckelträger", an dem die Einzelverschlüsse 3 für sich so lösbar angebracht sind, daß sie auch bei an den Reaktionsgefäßen 2 anliegendem Verschlußträger 6 einzeln herausgenommen werden können. Das erlaubt die einzelne Öffnung jedes Reaktionsgefäßes 2 bei im übrigen verschlossenen anderen Reaktionsgefäßen 2. Das hat die im allgemeinen Teil der Beschreibung erläuterten handhabungstechnischen Vorteile und vermeidet insbesondere jedwede Kreuzkontamination.Figs. 2, 3 and 4 can be seen in connection now that in the teaching of Invention is provided that the closure portions 4 of the individual closures 3 are plugged through the receptacles 7 in the closure carrier 6 therethrough, So when inserting and pulling out of the closure carrier 6 not collide with the closure carrier 6. With the operating sections 5 are the individual fasteners 3 further attached to the closure carrier 6, that at on the reaction vessels 2 attached closure carrier 6 together with individual closures 3 each individual closure 3 also individually from the closure carrier 6 and from the associated reaction vessel 2 is removable. In other words According to the invention, the closure carrier 6 is an independent "lid carrier" the individual closures 3 are so releasably attached to them that they too individually taken out in fitting to the reaction vessel 2 closure carrier 6 can be. This allows the single opening of each reaction vessel 2 with otherwise closed other reaction vessels. 2 This has the technical handling explained in the general part of the description Advantages and in particular avoids any cross-contamination.

Für die Befestigung der Einzelverschlüsse 3 am Verschlußträger 6 im Rahmen der Lehre gibt es natürlich verschiedene Möglichkeiten. Das dargestellte und insoweit bevorzugte Ausführungsbeispiel zeigt dabei, daß die Einzelverschlüsse 3 am Verschlußträger 6 mittels einer lösbaren Formschlußverbindung 8, 9 zwischen Betätigungsabschnitt 5 und Aufnahme 7 befestigt sind. Dies erkennt man besonders gut bei einer Zulsanunenschau der Fig. 2, 3 und 4. Die seitlichen Abmessungen der Aufnahme 7 und des Befestigungsabschnittes 5 des Einzelverschlusses 3 sind im dargestellten und bevorzugten Ausführungsbeispiel so gewählt und die Formschlußverbindung 8, 9 ist so gestaltet, daß sie bei in die Aufnahme 7 eingesetztem Einzelverschluß 3 eine geringfügige seitliche Verschiebung des Einzelverschlusses 3 in der Aufnahme 7 erlaubt. Diese geringfügige translatorische Verlagerungsmöglichkeit erlaubt eine genaue Positionierung der Verschlußabschnitte 4 aller Einzelverschlüsse 3 in den jeweiligen Reaktionsgefäßen 2. Dies gilt insbesondere auch dann, wenn man beim Aufsetzen des mit den Einzelverschlüssen 3 bestückten Verschlußträgers 6 die Reaktionsgefäße 2 reihenweise fortschreitend schließt. Die geringfügige zulässige Relativverschiebung zwischen Verschlußträger 6 und Einzelverschluß 3 erleichtert diese Verfahrensweise insbesondere dann, wenn man für den Verschlußträger 6 ein relativ steifes Kunststoffmaterial verwendet.For the attachment of the individual closures 3 on the closure carrier 6 in the frame Of course there are different possibilities for teaching. The illustrated and In this respect preferred embodiment shows that the individual closures 3 on the closure carrier 6 by means of a releasable positive connection 8, 9 are fixed between the operating section 5 and receptacle 7. This can be seen particularly well in a Zulsanunenschau of Fig. 2, 3 and 4. Die lateral dimensions of the receptacle 7 and the attachment portion. 5 of the single closure 3 are in the illustrated and preferred embodiment chosen and the positive connection 8, 9 is designed so that they are inserted into the receptacle 7 single closure 3 a slight lateral displacement of the single closure 3 in the receptacle 7 allowed. This slight translational displacement possibility allows a precise positioning of the closure portions 4 of all individual closures 3 in the respective reaction vessels 2. This is especially true then, if you when placing the stocked with the individual fasteners 3 closure carrier 6 the reaction vessels 2 in rows progressively closes. The slight permissible relative displacement between closure carrier. 6 and single closure 3 facilitates this procedure, in particular, if you for the closure carrier 6, a relatively rigid plastic material used.

In den Fig. 2, 3 und 4 erkennt man für die Formschlußverbindung 8, 9 im einzelnen, daß diese hier bajonettverschlußartig ausgeführt ist, wobei der Zapfenteil 8 der Formschlußverbindung 8, 9 am Einzelverschluß 3 angeordnet ist, während der Kulissenteil 9 der Formschlußverbindung 8, 9 die Aufnahme 7 des Verschlußträgers 6 bildet. Man erkennt die Kulissenteile 9 als Ränder der Aufnahmen 7 im Verschlüßträger 6 in Fig. 3. Man kann nachvollziehen, wie die Zapfenteile 8 am Betätigungsabschnitt 5 des Einzelverschlusses 3 in Fig. 4 in den entsprechenden Kulissenteilen 9 an der zugehörigen Aufnahme 7 bajonettverschlußartig laufen. In einer Drehstellung ist daher die Formschlußverbindung 8, 9 im dargestellten Ausführungsbeispiel geschlossen, in einer um 45° versetzten Drehstellung ist die Formschlußverbindung 8, 9 geöffnet, der Einzelverschluß 3 kann aus der Aufnahme 7 im Verschlußträger 6 nach oben herausgenommen werden. Im vorliegenden Fall sind vier Zapfenteile 8 an einem Betätigungsabschnitt 5 vorgesehen, man kann natürlich auch mit einer anderen Anzahl arbeiten. Die Anzahl der Zapfenteile 8 und Kulissenteile 9 bestimmt den zum Lösen oder Verschließen erforderlichen Drehwinkel.2, 3 and 4 can be seen for the positive connection 8, 9 in detail, that this is executed here bayonet-like closure, wherein the Spigot 8 of the positive connection 8, 9 arranged on the single closure 3 is, while the slide member 9 of the positive connection 8, 9 the recording 7 of the closure carrier 6 forms. You can see the slide parts 9 as Edges of the receptacles 7 in Verschlüßträger 6 in Fig. 3. It can be understood like the pin parts 8 on the actuating portion 5 of the single closure 3 in Fig. 4 in the corresponding link parts 9 at the associated Recording 7 bayonet-like run. In a rotational position is therefore closed the positive connection 8, 9 in the illustrated embodiment, in a rotational position offset by 45 ° is the positive connection 8, 9 open, the single closure 3 can from the receptacle 7 in the closure carrier 6 are taken upwards. In the present case four pin parts 8 are provided on an actuating portion 5, one Of course, you can also work with a different number. The number of Pin parts 8 and 9 parts set determines the release or closing required rotation angle.

Ein normaler Bajonettverschluß erreicht seine Schließstellung durch eine Drehbewegung in einer Richtung und seine Öffnungsstellung durch eine Drehbewegung in der entgegengesetzten Richtung. Ein solcher Bajonettverschluß kann für die Formschlußverbindung 8, 9 auch bei der Erfindung eingesetzt werden. Das bevorzugte Ausführungsbeispiel zeigt jedoch eine Konstruktion, bei der der Bajonettverschluß in beiden Drehrichtungen betätigbar ist und keinen Anschlag, sondern eine überwindbare Rastung 8a aufweist. Man erkennt die Rastung 8a an jedem Zapfenteil 8 in Fig. 5. Mit dieser Konstruktion ist die Bedienungsperson frei, in welcher Drehrichtung sie den Bajonettverschluß betätigen will, sie erreicht immer die jeweils andere Funktionsstellung des Bajonettverschlusses.A normal bayonet lock reaches its closed position by a Rotary movement in one direction and its opening position by a Rotary motion in the opposite direction. Such a bayonet lock can also be used in the invention for the positive connection 8, 9 become. However, the preferred embodiment shows a construction in which the bayonet lock in both directions can be actuated is and has no stop, but a surmountable detent 8a. It can be seen the detent 8a on each pin part 8 in Fig. 5. With this construction the operator is free, in which direction they the bayonet lock wants to operate, it always reaches the other functional position of the bayonet lock.

Als Alternativen kommt beispielsweise eine Quickverschraubung, beispielsweise eine Viertelkreisverschraubung, als Formschlußverbindung 8, 9 ebenso in Frage wie eine Art Schnappverschluß.As alternatives comes for example a Quickverschraubung, for example a Viertelkreisverschraubung, as positive connection 8, 9 as well in question as a kind of snap closure.

Grundsätzlich ist es auch möglich, die Einzelverschlüsse 3 am Verschlußträger 6 mittels einer Reibschlußverbindung zu befestigen. Der Reibschluß einer solchen Reibschlußverbindung sollte dann allerdings so groß sein, daß der Verschlußträger 6 mit allen Einzelverschlüssen 3 ohne weiteres auf die Reaktionsgefäße 2 dichtend aufgesetzt und von diesen wieder abgezogen werden kann. Weil diese Kraftverhältnisse schwierig richtig abzustimmen sind, was man bereits von den bekannten Silikonmatten als Sammelverschlüsse kennt (WO 99/44.747), wird man in der Praxis wohl die auch bei dem Ausführungsbeispiel realisierten Formschlußverbindungen 8, 9 wählen. In principle, it is also possible, the individual closures 3 on the closure carrier 6 fasten by means of a frictional connection. The friction of a However, such a frictional connection should then be so great that the Closure carrier 6 with all individual closures 3 readily on the reaction vessels 2 sealed and removed from these again can. Because these power relations are difficult to tune properly, which one already knows of the known silicone mats as collecting closures (WO 99/44747), you will probably in practice also in the embodiment realized form-fitting connections 8, 9 choose.

Fig. 2 deutet an und Fig. 5 zeigt auch beim Einzelverschluß 3 deutlicher, daß der Einzelverschluß 3, insbesondere und hier dargestellt am Betätigungsabschnitt 5, ein Verschlußhandhabungsmittel 10 zum Angriff eines der Handhabung eines einzelnen Einzelverschlusses 3 dienenden Betätigungswerkzeugs 11 aufweist. Im dargestellten Ausführungsbeispiel ist das Betätigungswerkzeug 11 als stiftartiges Handwerkzeug angedeutet. Man wird aber für umfangreichere Handhabungen häufig auch automatisch bewegte und betätigte Betätigungswerkzeuge 11 einsetzen, wie das für sich aus dem Stand der Technik bekannt ist.Fig. 2 indicates and Fig. 5 also shows the single closure 3 more clearly that the single closure 3, in particular and shown here on the operating section 5, a closure handling means 10 for attacking one of the handling a single single closure 3 serving operating tool 11 has. In the illustrated embodiment, the actuating tool 11 indicated as a pin-like hand tool. But one is for more extensive Handling often also automatically moved and operated Insert actuation tools 11, how to stand out from the stand Technique is known.

Im dargestellten Ausführungsbeispiel ist vorgesehen, daß das Verschlußhandhabungsmittel 10 als Formschlußelement ausgeführt ist, zu dem ein entsprechendes Formschlußelement 12 am Betätigungswerkzeug 11 korrespondiert. Genauer gesagt ist im dargestellten Ausführungsbeispiel das Verschlußhandhabungsmittel 10 als ein Teil, nämlich hier als Kulissenteil, eines Bajonettverschlusses ausgeführt. Das Formschlußelement 12 am Betätigungswerkzeug 11 ist als der andere Teil, insbesondere der Zapfenteil des Bajonettverschlusses ausgeführt.In the illustrated embodiment, it is provided that the closure handling means 10 is designed as a form-locking element, to which a corresponding Positive locking element 12 corresponds to the actuating tool 11. More specifically, in the illustrated embodiment, the shutter handling means 10 as a part, namely here as a sliding part, a bayonet closure executed. The form-locking element 12 on the actuating tool 11 is as the other part, in particular the pin part of the bayonet closure executed.

Für die Ausgestaltung des Verschlußhandhabungsmittels 10 und des entsprechenden Formschlußelements 12 zeigt das dargestellte Ausführungsbeispiel eine übliche, klassische Bajonettverschluß-Konstruktion. Es sollte insoweit zweckmäßigerweise vorgesehen sein, daß nach Herstellung des Bajonettverschlußeingriffs eine Drehung des in die Aufnahme 7 eingesetzten Einzelverschlusses 3 in beiden Drehrichtungen wirksam möglich ist, so daß die erfindungsgemäß bei dem bevorzugten Ausführungsbeispiel verwirklichte Rechts/Links-Unempfindlichkeit der Formschlußverbindung 8, 9 auch ausgenutzt werden kann.For the embodiment of the closure handling means 10 and the corresponding Positive locking element 12 shows the illustrated embodiment a standard, classic bayonet closure construction. It should be so far expediently provided that after production of the bayonet closure engagement a rotation of the individual closure used in the receptacle 7 3 is effectively possible in both directions, so that the present invention realized in the preferred embodiment Right / left insensitivity of the positive connection 8, 9 also exploited can be.

Auch für die Handhabung der Einzelverschlüsse 3 gelten im übrigen die sonstigen Alternativen sei es in formschlüssiger Ausführung, sei es in reibschlüssiger Ausführung, die zuvor bereits erläutert worden sind.For the handling of the individual closures 3 also apply the rest Alternatives be it in form-fitting execution, be it in frictional Embodiment, which have already been explained above.

Das dargestellte und bevorzugte Ausführungsbeispiel zeigt insbesondere in den Fig. 4 und 5, daß am Einzelverschluß 3 die Formschlußverbindung 8, 9 oder Reibschlußverbindung außen am Betätigungsabschnitt 5 angeordnet ist, während das Verschlußhandhabungsmittel 10 des Einzelverschlusses 3 innen angeordnet ist. Das korrespondiert zu der in den Fig. 2 und 3 erkennbaren Anordnung der Einzelverschlüsse 3 in Einbettung in die Aufnahmen 7 im Verschlußträger 6.The illustrated and preferred embodiment shows in particular FIGS. 4 and 5, that the single closure 3, the positive connection 8, 9 or frictional connection is arranged on the outside of the actuating section 5, while the closure handling means 10 of the single closure 3 inside is arranged. This corresponds to that in FIGS. 2 and 3 recognizable Arrangement of the individual closures 3 in embedding in the receptacles 7 in Closure carrier 6.

Hinsichtlich des Zusammenwirkens des Verschlußabschnittes 4 eines Einzelverschlusses 3 mit dem offenen Ende des zugehörigen Reaktionsgefäßes 2 kann, wie im Stand der Technik, vorgesehen sein, daß der Verschlußabschnitt 4 des Einzelverschlusses 3 das Reaktionsgefäß 2 überfaßt. Das dargestellte und bevorzugte Ausführungsbeispiel zeigt allerdings eine Ausführung, die dadurch gekennzeichnet, ist, daß der Verschlußabschnitt 4 des Einzelverschlusses 3 in das offene Ende des Reaktionsgefäßes 2 stopfenartig eintritt. Auch das ist für sich aus dem Stand der Technik bekannt. Das erlaubt eine besonders zweckmäßige, hier im Ausführungsbeispiel auch verwirklichte Konzeption dergestalt, daß die Befestigungsabschnitte 5 der Einzelverschlüsse 3 aus einem relativ harten, steifen Kunststoffmaterial bestehen, die Verschlußabschnitte 4 daran materialschlüssig angeformt sind und aus einem relativ weichen, gummielastischen Kunststoffmaterial bestehen. Fig. 4 zeigt dies angedeutet durch die unterschiedliche Graustufe der beiden Abschnitte 4, 5. Damit wird eine optimale Dichtwirkung im Verschlußabschnitt 4 erreicht und gleichwohl eine einfache, zweckmäßige Handhabbarkeit der Einzelverschlüsse 3 in Verbindung mit dem Verschlußträger 6 gewährleistet. Dieser sollte nämlich auch aus einem relativ harten, steifen Kunststoffmaterial bestehen, um den Verschlußträger 6 insgesamt gut handhaben und insbesondere mit der Mikrotiterplatte 1 und deren Reaktionsgefäßen 2 leicht verbinden zu können.With regard to the interaction of the closure portion 4 of a single closure 3 with the open end of the associated reaction vessel. 2 can, as in the prior art, be provided that the closure portion 4 of the single closure 3, the reaction vessel 2 overlapped. The illustrated and preferred embodiment, however, shows an embodiment which characterized in that the closure portion 4 of the single closure 3 enters the open end of the reaction vessel 2 like a plug. This, too, is known per se from the prior art. That allows one particularly expedient, realized here in the embodiment also Conception in such a way that the fastening portions 5 of the individual closures 3 consist of a relatively hard, rigid plastic material, the Closure sections 4 are integrally formed materially and from a consist of relatively soft, rubber-elastic plastic material. Fig. 4 shows this indicated by the different gray level of the two sections 4, 5. Thus, an optimal sealing effect in the closure portion 4 is achieved and nevertheless a simple, convenient handling of the individual closures 3 ensured in conjunction with the closure carrier 6. This should be made of a relatively hard, stiff plastic material, to handle the closure carrier 6 overall good and in particular easily connect to the microtiter plate 1 and its reaction vessels 2 can.

Nicht dargestellt ist in der Zeichnung eine Ausführung, die ein leichtes Abtropfen von Kondensat von der Unterseite des Verschlußabschnittes 4 dadurch erlaubt, daß dieser nach unten gewölbt ausgeführt ist.Not shown in the drawing is an embodiment that drips easily condensate from the bottom of the closure portion 4 thereby allows it to be curved downwards.

Zum Stand der Technik ist erläutert worden, daß auch Einzelverschlüsse 3 bekannt sind, die eine dünne Membran aufweisen, durch die das Innere des jeweiligen Reaktionsgefäßes mit einer Nadel erreichbar ist. Das dargestellte und bevorzugte Ausführungsbeispiel zeigt eine ebensolche Konstruktion, nämlich eine Konstruktion, bei der der Verschlußabschnitt 4 einen durchstechbaren Membranabschnitt 4a aufweist. Dieser ist durch einen mittigen Durchtritt 5a im Befestigungsabschnitt 5 erreichbar. Beides zeigt Fig. 5. Die Ausführung des Verschlußabschnittes 4 aus einem relativ weichen, gummielastischen Kunststoffmaterial, insbesondere aus einem thermoplastischen Elastomer, bietet die zweckmäßige Möglichkeit, daß sich ein Durchstich im Membranabschnitt 4a des Verschlußabschnittes 4 von selbst wieder schließt, weil das thermoplastische Elastomer eine hinreichende Rückstellkraft hat.The prior art has been explained that even individual closures. 3 are known, which have a thin membrane through which the interior of the each reaction vessel can be reached with a needle. The illustrated and preferred embodiment shows a similar construction, namely a construction in which the closure portion 4 is a pierceable Membrane section 4a has. This one is through a central one Passage 5a reachable in the attachment section 5. Both show Fig. 5. The Execution of the closure portion 4 of a relatively soft, rubber-elastic Plastic material, in particular of a thermoplastic elastomer, offers the convenient possibility that a puncture in the Diaphragm section 4a of the closure section 4 closes again by itself, because the thermoplastic elastomer has a sufficient restoring force.

Natürlich kann man bei Wahl unterschiedlichen Materials dieses auch unterschiedlich einfärben oder auch die Einzelverschlüsse 3 anders einfärben als den Verschlußträger 6. Natürlich können auch die Einzelverschlüsse 3 untereinander unterschiedliche Farben haben, beispielsweise um damit Informationen über den Inhalt des jeweiligen Rekationsgefäßes 2 zu verbinden. Letzteres deutet Fig. 2 mit den unterschiedlichen Graustufen der beiden dort erkennbaren Einzelverschlüsse 3 an.Of course you can also choose different materials this different colorize or color the individual closures 3 differently than Of course, the individual closures 3 with each other have different colors, for example, to provide information to connect via the content of the respective Rekationsgefäßes 2. The latter indicates Fig. 2 with the different gray levels of the two there recognizable individual closures 3 on.

Fig. 6 zeigt in vergrößerter Darstellung schließlich das Betätigungswerkzeug 11 mit dem darin befindlichen Formschlußelement 12 und einem evtl. federbelasteten Druckzapfen 13, der die Fixierung des Bajonettverschlusses zum Verschlußhandhabungsmittel 10 des Einzelverschlusses 3 leistet. Natürlich sind viele andere Konstruktionen für entsprechende Betätigungswerkzeuge 11 denkbar. Man kann auch vorsehen, daß die Federkraft zur Fixierung des Bajonettverschlusses durch die Eigenelastizität des Verschlußabschnittes 4 aufgebracht wird. Im dargestellten Ausführungsbeispiel steht dazu der Druckzapfen 13 in dem Durchtritt 5a unmittelbar auf dem Material des Verschlußabschnitts 4, so daß diese zusätzliche Funktion geleistet wird.Fig. 6 shows an enlarged view, finally, the operating tool 11 with the therein form-locking element 12 and a possibly spring-loaded Thrust pin 13, the fixation of the bayonet lock for Closure handling means 10 of the single closure 3 makes. Naturally There are many other designs for appropriate operating tools 11 conceivable. One can also provide that the spring force for fixing the Bayonet closure by the inherent elasticity of the closure portion. 4 is applied. In the illustrated embodiment is to the Thrust pin 13 in the passage 5a directly on the material of the closure portion 4, so that this additional function is performed.

Fig. 3 zeigt den Verschlußträger 6 einer erfindungsgemäßen Anordnung für sich, Fig. 2 zeigt den Verschlußträger 6 bestückt mit Einzelverschlüssen 3. Solche Verschlußträger sind auch für sich selbst handelsfähige Elemente, die daher einem eigenständigen Schutz unterliegen. Entsprechendes gilt für die Einzelverschlüsse 3.Fig. 3 shows the closure carrier 6 of an inventive arrangement for 2 shows the closure carrier 6 equipped with individual closures 3. Such closure carrier are also self-negotiable elements, the therefore be subject to independent protection. The same applies to the Single closures 3.

Fig. 7 zeigt schematisch angedeutet ein Beispiel einer Vorrats- und Abgabeanordnung 14 für Einzelverschlüsse 3 der in Rede stehenden Art. Diese ist mit einer eigenen Formschlußausbildung 15 ausgestattet, die mit der Formschlußausbildung, nämlich den Zapfenteilen 8, am Betätigungsabschnitt 5 des Einzelverschlusses 3 dergestalt zusammenwirkt, daß die Einzelverschlüsse 3 in genau vorgegebener, bestimmter lateraler Ausrichtung aus der Vorrats- und Abgabeanordnung 14 abgebbar sind. So kann man gezielt eine Bestückung entweder des Verschlußträgers 6 mit den Einzelverschlüssen 3 bewerkstelligen oder auch direkt eine Bestückung der Reaktionsgefäße 2 ohne Verschlußträger 6.Fig. 7 shows schematically an example of a supply and dispensing arrangement 14 for individual closures 3 of the type in question. This is equipped with its own form-locking formation 15, with the form-locking education, namely the pin parts 8, on the operating section. 5 the single closure 3 cooperates in such a way that the individual closures 3 in exactly predetermined, certain lateral orientation of the Supply and dispensing arrangement 14 can be dispensed. So you can specifically a Equipping either the closure carrier 6 with the individual closures. 3 accomplish or even directly equipping the reaction vessels. 2 without closure carrier 6.

Die erfindungsgemäße Anordnung läßt sich besonders zweckmäßig innerhalb eines Industrie-Robotersystems verwenden, mit dem Reaktionsabläufe der in Rede stehenden Art serienweise und im wesentlichen automatisch geprüft werden. In diesem Zuge kann beispielsweise das Betätigungswerkzeug 11 Teil des Industrie-Robotersystems sein, beispielsweise an einem Betätigungsarm angeordnet, wobei das Industrie-Robotersystem auch die Reaktionsgefäße 2 und den Verschlußträger 3 automatisch handhabt, zumindest betätigbar fixiert.The arrangement according to the invention can be particularly useful within of an industrial robot system, with the reaction sequences of in Speech type in series and essentially automatically checked become. In this course, for example, the operating tool 11th Be part of the industrial robot system, for example on an actuating arm arranged, whereby the industrial robot system also the reaction vessels 2 and the closure carrier 3 automatically handles, at least operable fixed.

Gegenstand der Lehre ist auch eine Verwendung einer Anordnung der in Rede stehenden Art in einem Industrie-Robotersystem.The teaching is also a use of an arrangement of in Talking style in an industrial robot system.

Claims (19)

  1. Arrangement for the contamination-free processing of reaction sequences, in particular molecular-biological reaction sequences, comprising
    a number of reaction wells (2) which are arranged next to one another, are connected to one another and are open at the top,
    an individual closure (3) for each reaction well (2) with a closure portion (4) for the sealed closure of the opening of the reaction well (2) and an actuating portion (5) for engaging the individual closure (3) and
    a closure carrier (6), covering all the reaction wells (2) or groups of reaction wells (2), with a respective receptacle (7) for the actuating portion (5) of each individual closure (3) for fastening the individual closure (3) on the closure carrier (6),
    it being possible for the individual closures (3) that are fastened on the closure carrier (6) to be fitted in a sealing manner together with the closure carrier (6) onto the reaction wells (2) and pulled off from them,
    characterized in that the closure portions (4) of the individual closures (3) can be made to pass through the receptacles (7) in the closure carrier (6) in both directions and
    in that the individual closures (3) are fastened on the closure carrier (6) in such a way that, when the closure carrier (6) with individual closures (3) is fitted on the reaction wells (2), each individual closure (3) can be individually removed from the closure carrier (6) and from the reaction well (2).
  2. Arrangement according to Claim 1, characterized in that the reaction wells (2) are grouped together in a microtiter plate.
  3. Arrangement according to Claim 1 or 2, characterized in that the individual closures (3) are fastened on the closure carrier (6) by means of a releasable positively locking connection (8, 9) between the actuating portion (5) and the receptacle (7) and, preferably, in that the positively locking connection (8, 9) is formed in such a way that it allows slight lateral displacement of the individual closure (3) in the receptacle (7) when the individual closure (3) is fitted in the receptacle (7).
  4. Arrangement according to Claim 3, characterized in that the positively locking connection (8, 9) is configured as a bayonet closure or as a quick screw connection or as a snap closure.
  5. Arrangement according to Claim 4, characterized in that, with the positively locking connection (8, 9) configured as a bayonet closure, the pin part (8) of the positively locking connection (8, 9) (is arranged on the individual closure (3) and the slot part (9) of the positively locking connection (8, 9) is arranged on the closure carrier (6) and/or in that the positively locking connection (8, 9) configured as a bayonet closure can be actuated in both directions of rotation and does not have a stop but instead a latching (8a) that can be overcome.
  6. Arrangement according to Claim 1 or 2, characterized in that the individual closures (3) are fastened on the closure carrier (6) by means of a frictionally locking connection between the actuating portion (5) and the receptacle (7).
  7. Arrangement according to one of Claims 1 to 6, characterized in that the individual closures (3) have, in particular on the actuating portion (5), a closure handling means (10) for the engagement of an actuating tool (11) serving for the handling of a single individual closure (3).
  8. Arrangement according to Claim 7, characterized in that the closure handling means (10) is configured as a positively locking element, to which there corresponds a corresponding positively locking element (12) on the actuating tool (11), or in that the closure handling means (10) is configured as part of a quick screw connection or in that the closure handling means (10) is configured as part of a snap closure or in that the closure handling means (10) is configured as a frictionally locking element, to which there corresponds a corresponding frictionally locking element on the actuating tool (11).
  9. Arrangement according to Claim 8, characterized in that the closure handling means (10) is configured as one part, in particular the slot part, and the positively locking element (12) on the actuating tool (11) is configured as the other part, in particular the pin part, of a bayonet closure and, preferably, in that the bayonet closure (10, 12) is configured in such a way that, after establishing the bayonet closure engagement, it allows rotation of the individual closure (3) inserted into the receptacle (7) in both directions of rotation.
  10. Arrangement according to one of Claims 3 to 6 and one of Claims 7 to 9, characterized in that the positively locking connection (8, 9) or the frictionally locking connection is arranged on the outside of the actuating portion (5) of the individual closure (3) and the closure handling means (10) is arranged on the inside of the individual closure (3).
  11. Arrangement according to one of Claims 1 to 10, characterized in that the closure portion (4) of the individual closure (3) engages over the reaction well (2) or in that the closure portion (4) of the individual closure (3) enters the reaction well (2) in the manner of a plug.
  12. Arrangement according to one of Claims 1 to 11, characterized in that the closure portion (4) is configured in a downwardly convex manner and/or in that the closure portion (4) has a pierceable membrane portion (4a) and/or in that the fastening portion (5) of the individual closure (3) consists of a relatively hard, rigid plastics material and the closure portion (4) is formed on it with a material bond and consists of a relatively soft, elastomeric plastics material, in particular a thermoplastic elastomer, the fastening portion (5) preferably having a central passage (5a), which reaches as far as the closure portion (4), and/or in that the individual closures (3) and the closure carrier (6) consist of relatively hard, rigid plastics material.
  13. Arrangement according to one of Claims 7 to 12, characterized in that the actuating tool (11) is part of an industrial robot system, by which the reaction wells (2) and the closure carrier (6) can also be automatically handled.
  14. Use of an arrangement according to one of Claims 1 to 12 in an industrial robot system.
  15. Closure carrier with individual closures for an arrangement according to one of Claims 1 to 12,
    with a respective receptacle (7) for an actuating portion (5) of a individual closure (3) for fastening the individual closure (3) on the closure carrier (6),
    characterized by
    a formation of the receptacles (7) as a positively locking element of a positively locking connection (8, 9) or as a frictionally locking element of a frictionally locking connection.
  16. Closure carrier according to Claim 13, characterized by the features of the characterizing clauses of one or more of Claims 3 to 5 and/or by being formed from relatively hard, rigid plastics material.
  17. Individual closure for a reaction well (2) of an arrangement for the contamination-free processing of reaction sequences, in particular molecular-biological reaction sequences, in particular for an arrangement according to one of Claims 1 to 12,
    with a closure portion (4) for the sealed closure of the opening of an assigned reaction well (2) and an actuating portion (5) for engaging and handling the individual closure (3),
    characterized in that a positively locking element of a positively locking connection (8, 9) or a frictionally locking element of a frictionally locking connection is arranged on the actuating portion (5).
  18. Individual closure according to Claim 15, characterized by the features of the characterizing clause of one or more of Claims 3 to 5 and also 7 to 12.
  19. Storage and dispensing arrangement for individual closures for an arrangement for the contamination-free processing of reaction sequences, in particular molecular-biological reaction sequences, in particular for an arrangement according to one of Claims 1 to 12,
    characterized in that it has a positively locking formation (15),
    in that the positively locking formation (8) on the actuating portion (5) of the individual closure (3) interacts with this positively locking formation (15) on the storage and dispensing arrangement (14) in such a way that the individual closures (3) can be dispensed from the storage and dispensing arrangement (14) in a predetermined, specific lateral alignment.
EP01104986A 2000-03-09 2001-03-01 Individual closure and closure carrier for reaction wells, storage and dispensing device for individual closures Expired - Lifetime EP1132135B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10013240 2000-03-09
DE10013240A DE10013240A1 (en) 2000-03-09 2000-03-09 Arrangement for contamination-free processing of, in particular, molecular biological reaction sequences, closure carrier and individual closure for such an arrangement, and storage and dispensing arrangement for individual closures

Publications (3)

Publication Number Publication Date
EP1132135A2 EP1132135A2 (en) 2001-09-12
EP1132135A3 EP1132135A3 (en) 2003-06-25
EP1132135B1 true EP1132135B1 (en) 2005-08-03

Family

ID=7635258

Family Applications (1)

Application Number Title Priority Date Filing Date
EP01104986A Expired - Lifetime EP1132135B1 (en) 2000-03-09 2001-03-01 Individual closure and closure carrier for reaction wells, storage and dispensing device for individual closures

Country Status (5)

Country Link
US (1) US20010036426A1 (en)
EP (1) EP1132135B1 (en)
JP (1) JP2001330537A (en)
AT (1) ATE301000T1 (en)
DE (2) DE10013240A1 (en)

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Also Published As

Publication number Publication date
EP1132135A3 (en) 2003-06-25
JP2001330537A (en) 2001-11-30
EP1132135A2 (en) 2001-09-12
DE10013240A1 (en) 2001-10-11
DE50106928D1 (en) 2005-09-08
US20010036426A1 (en) 2001-11-01
ATE301000T1 (en) 2005-08-15

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