EP1129345A1 - Dispositif pratique permettant d'agir sur un flux de volume ultra-reduit - Google Patents

Dispositif pratique permettant d'agir sur un flux de volume ultra-reduit

Info

Publication number
EP1129345A1
EP1129345A1 EP99958906A EP99958906A EP1129345A1 EP 1129345 A1 EP1129345 A1 EP 1129345A1 EP 99958906 A EP99958906 A EP 99958906A EP 99958906 A EP99958906 A EP 99958906A EP 1129345 A1 EP1129345 A1 EP 1129345A1
Authority
EP
European Patent Office
Prior art keywords
capillary channel
voltage
flow
capillary
integrated external
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP99958906A
Other languages
German (de)
English (en)
Inventor
Mark A. Hayes
Nolan A. Polson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Arizona
Original Assignee
University of Arizona
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Arizona filed Critical University of Arizona
Publication of EP1129345A1 publication Critical patent/EP1129345A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44791—Microapparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44752—Controlling the zeta potential, e.g. by wall coatings

Definitions

  • electrophoresis and in particular to a device for controlling the movement of fluids in
  • a capillary channel used in chemical systems for separations, reactions, or analysis.
  • Microdevices for fluids Movement of fluids on microchips has been
  • valves have not been fabricated on the micron to sub-micron scale
  • Electroosmosis is the most important flow-generating mechanism
  • the ⁇ -potential is
  • the cationic counter ions (H 3 O + , Na + typically) entrained in the diffuse layer are free to migrate towards the
  • Electroosmosis also termed electroosmotic flow
  • electroosmosis as a propulsion mechanism is that both the flow rate and the
  • Electroosmosis is also an important component of capillary zone
  • the flow generated is usually large enough to force all species present
  • Electroosmosis directly influences the efficiency
  • Electroosmosis can be altered in a variety of ways. Examples of
  • EAF electroosmotic flow
  • capillary for fused silica capillaries used in conventional capillary electrophoresis
  • Radial voltage flow control Radial voltage flow control, a method to
  • heterogeneous (-potential caused by radial fields in the partially covered capillaries.
  • radial voltage flow control could be done at lower voltages in a silica capillary of up
  • micrometer inside diameter having an inner capillary of 75 micrometer outside
  • micrometer inside diameter channel cross section 2 x 10 " ° square meters
  • section 2 x 10 "9 square meters) and 370 micrometer outside diameter, in which radial voltages of up to 30 kilovolts were applied across the capillary to control
  • channel inner wall surfaces were 62 and 160 micrometers, respectively.
  • micrometer inside diameter channel cross section 0.3 x 10 "9 square meters
  • radial voltage electrode and the channel inner wall surface was 62 micrometers.
  • invention is to provide a device and method for monitoring uniform electroosmotic
  • an integrated external electrode positioned microscopically close to a capillary
  • ultrasmall cross section of the capillary channel reduces the voltage required for
  • the present invention is directed to a capillary
  • electrodes are positioned at the immediate ends of the channel to apply a longitudinal
  • This embodiment permits independent control of the
  • the present invention is directed to a capillary
  • dielectric constant is positioned between the integrated external electrode and the capillary wall to inject charge to the capillary channel inner wall surface when voltage
  • a plurality of channels are combined in a device.
  • the present invention is directed to a
  • capillary channel device as described above, further comprising a means to monitor
  • Fig. 1 illustrates an example of a device for ultrasmall volume flow
  • Fig. 2 illustrates the physical parameters of geometry of a device for
  • Fig. 3 illustrates a plot of model capillary inner wall surface charge
  • Fig. 4 illustrates an example of a microchip device for ultrasmall
  • Fig. 5. illustrates a plot of fluorescent intensity of a dye migrating
  • Fig. 6. illustrates an example of a device for ultrasmall volume flow
  • integrated external electrode we mean an electrical conductor
  • the perpendiclar voltage field provides control of
  • the present invention provides a practical device for controlling
  • Fluid flow is provided in a capillary channel 170 defined by
  • control electroosmotic flow is applied perpendicularly across a capillary channel
  • a voltage to the integrated external electrode is provided.
  • a voltage to the integrated external electrode is provided.
  • electrodes 110 are provided at the immediate ends 180 of the capillary channel to
  • longitudinal electrodes can be electrically connected to nodes 100 for connecting to a
  • the longitudinal electrodes 110 can be adjacent to, and in electrical contact
  • This device will find application, for example, with capillary zone
  • electrophoresis Another example is any fluid movement within microinstrumentation
  • the device can be made as a microchip, as shown in Fig. 2.
  • capillary channel 170 is again defined by the substrate 160, and can have ultrasmall
  • Integrated external electrodes 120 can be positioned
  • the device can be a ceramic, silica, fused silica, quartz, a silicate, a titanate, a metal oxide,
  • nitride silicon, titanium dioxide, and the like, or a polymer, a plastic, a
  • polydimethylsiloxane or a polymethylmethacrylate.
  • the applied voltages may be lower in
  • the first issue is structural integrity.
  • electrode or conductor, more accurately
  • electrode could be placed very near (nanometers to
  • Fig. 4 wherein a microchip capillary channel device is illustrated.
  • substrate 160 defines a capillary channel 170.
  • Two integrated external electrodes 120 are integrated external electrodes 120
  • a material of high dielectric constant 130 can be
  • Ultrasmall capillary channel cross section A fused silica capillary
  • tube may be modeled as a cylindrical capacitor, as described in Keely, et al., Chromatogr. A 1993, 652, 283-289. Without intending to be bound by any one
  • capillary channel wall can also improve the control of flow. They can increase the
  • a material with high dielectric constant such as titanium
  • the typical substrate material quartz (or fused silica) has a
  • ⁇ material are ceramics, a silicate, a titanate, a metal oxide, a nitride, titanium dioxide,
  • the direction in which the electric charge is transferred can be any direction in which the electric charge is transferred.
  • the charge will be preferentially injected towards the channel.
  • the device is a combination of capillary channels each with
  • perpendicular voltage flow control as shown in Fig. 6, controlling the direction of
  • the distances can be 100 or more, and the ratio of the dielectric constants can be as
  • the surface must retain low surface charge density in the presence of the
  • aqueous buffers typically used in capillary electrophoresis as described in Poppe, et
  • the surface charge density should be insensitive to pH
  • the silicate surface is labile to acid and base
  • organosilanes forms an uncharged, stable surface, as described in Pesek, et al., Chromatographia 1997, 44, 538-544, which is hereby incorporated by reference in its
  • the organosilane coating on the titanium dioxide does not require hindered
  • buffer/wall interface must be minimized to extend radial voltage flow control to
  • Polymers have been covalently bound and physically adsorbed to the inner wall
  • triorganosilane treatments have demonstrated stability to acidic and basic buffers and
  • This information is used as a feedback mechanism to confirm or to
  • monitoring device is that the materials and fluid within the channel must remain
  • the monitoring system must be non-invasive
  • the flow may be calculated from the elution time. This technique is limited to
  • One method to directly measure EOF is to weigh the mass transferred
  • conductivity across the capillary is proportional to a weighted average of the
  • Patent No. 5,624,539 which is hereby incorporated by reference in its entirety.
  • channels, or selected channels, allow introduction of an electric field selectively
  • these longitudinal electrodes provide
  • Bulk flow can be directly changed by the applied longitudinal voltage field, or by changes in the (-potential caused by perpendicular
  • Electrophoretic migration may be changed by varying the longitudinal
  • Lucifer yellow was prepared (1 mg/mL) using NaH 2 PO 4 buffer. All
  • a capillary channel microdevice was designed in-
  • This device consisted of a long capillary channel, used for electrophoretic separation,
  • the substrate was Corning 0211 glass
  • the side channels were off-set by 500 micrometers.
  • Integrated external electrodes were positioned parallel to the main channel, separated
  • the effective perpendicular voltage field strength was determined by first
  • the effective perpendicular voltage field was the
  • Image acquisition was performed with an RSI 70 CCD camera (CSI
  • the device was approximately 40 times
  • Peak elution times varied by as much as 16 ⁇ 3 seconds over a 5 mm separation distance, as shown in
  • modified yellow-green fluorescent (505 nm excitation/515 nm emission) latex microspheres (Molecular Probes, Eugene, Oregon) were used as received. All
  • NaH 2 PO 4 buffers were prepared to 100 mM concentration and adjusted with 100 mM
  • the device was interfaced by placing the cathodic buffer reservoir in a
  • buffer reservoir was fashioned from plexiglas material to form a container where the
  • a substrate of Corning 0211 glass is fabricated defining a capillary
  • An integrated external electrode is positioned parallel to the channel separated by
  • a layer of titanium dioxide, a high dielectric material, is positioned between the integrated external electrode and
  • the channel extending longitudinally 0.2 cm in both directions from the longitudinal
  • a voltage is applied to the integrated external electrode to

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Dispersion Chemistry (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Electrostatic Separation (AREA)
  • Flow Control (AREA)

Abstract

Ce dispositif, permettant d'agir sur un débit fluidique de volume ultra-réduit, qui est utilisé dans les domaines de la séparation par électrophorèse, de l'analyse chimique et des réactions microchimiques, comporte un substrat définissant un canal capillaire et des électrodes extérieures intégrées destinées à commander le débit électro-osmotique. La géométrie du canal et la mise en place à proximité d'un électrode extérieure intégrée permettent de réduire la tension nécessaire pour agir sur le débit. Des électrodes longitudinales assurent la séparation électrophorétique des composants. La présence d'un matériau fortement diélectrique entre l'électrode extérieure intégrée et le capillaire permet de réduire la tension nécessaire pour agir sur le débit. Une surveillance en temps réel du débit et la présence d'un revêtement superficiel sur le canal capillaire améliorent cette régulation du débit.
EP99958906A 1998-11-12 1999-11-10 Dispositif pratique permettant d'agir sur un flux de volume ultra-reduit Withdrawn EP1129345A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10808698P 1998-11-12 1998-11-12
US108086P 1998-11-12
PCT/US1999/026724 WO2000028315A1 (fr) 1998-11-12 1999-11-10 Dispositif pratique permettant d'agir sur un flux de volume ultra-reduit

Publications (1)

Publication Number Publication Date
EP1129345A1 true EP1129345A1 (fr) 2001-09-05

Family

ID=22320213

Family Applications (1)

Application Number Title Priority Date Filing Date
EP99958906A Withdrawn EP1129345A1 (fr) 1998-11-12 1999-11-10 Dispositif pratique permettant d'agir sur un flux de volume ultra-reduit

Country Status (4)

Country Link
EP (1) EP1129345A1 (fr)
JP (1) JP2002529235A (fr)
CA (1) CA2348864A1 (fr)
WO (1) WO2000028315A1 (fr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7465382B2 (en) * 2001-06-13 2008-12-16 Eksigent Technologies Llc Precision flow control system
EP1362827B1 (fr) * 2002-05-16 2008-09-03 Micronit Microfluidics B.V. Méthode de fabrication d'un dispositif microfluidique
NL1021269C2 (nl) * 2002-08-14 2004-02-17 Lionix B V Elektrodesysteem en werkwijze voor het aanleggen van elektrische spanningen en het teweegbrengen van elektrische stromen.
JP2004276224A (ja) * 2003-03-17 2004-10-07 Toyo Technol Inc 繊維を充填した電気浸透ポンプ
JP4880944B2 (ja) * 2005-08-11 2012-02-22 セイコーインスツル株式会社 液体移動装置、マイクロリアクタ、およびマイクロリアクタシステム
JP4986504B2 (ja) * 2006-05-23 2012-07-25 愛知時計電機株式会社 電磁式流量計測装置
GB2477287B (en) * 2010-01-27 2012-02-15 Izon Science Ltd Control of particle flow in an aperture
SE534488C2 (sv) 2010-02-22 2011-09-06 Lunavation Ab Ett system för elektrokinetisk flödesteknik
FR3025440A1 (fr) * 2014-09-05 2016-03-11 Centre Nat Rech Scient Dispositif et procede d'analyse microfluidique
US11090660B2 (en) 2016-08-10 2021-08-17 Arizona Board Of Regents On Behalf Of Arizona State University Hyper efficient separations device
WO2019003230A1 (fr) * 2017-06-29 2019-01-03 Technion Research & Development Foundation Limited Dispositifs et procédés pour commander un flux à l'aide d'un flux électro-osmotique
EP3792623A1 (fr) * 2019-09-16 2021-03-17 Imec VZW Dispositif d'électrophorèse capillaire cyclique

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5092972A (en) * 1990-07-12 1992-03-03 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Field-effect electroosmosis
US5180475A (en) * 1991-09-04 1993-01-19 Hewlett-Packard Company System and method for controlling electroosmotic flow
US5358618A (en) * 1993-01-22 1994-10-25 The Penn State Research Foundation Capillary electrophoresis apparatus with improved electroosmotic flow control
US5415747A (en) * 1993-08-16 1995-05-16 Hewlett-Packard Company Capillary electrophoresis using zwitterion-coated capillary tubes
US5624539A (en) * 1995-06-19 1997-04-29 The Penn State Research Foundation Real time monitoring of electroosmotic flow in capillary electrophoresis
GB9805301D0 (en) * 1998-03-12 1998-05-06 Imperial College Detector

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO0028315A1 *

Also Published As

Publication number Publication date
WO2000028315B1 (fr) 2000-07-06
WO2000028315A1 (fr) 2000-05-18
CA2348864A1 (fr) 2000-05-18
JP2002529235A (ja) 2002-09-10

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