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• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
  • C12N15/09—Recombinant DNA-technology
  • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
  • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
  • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
  • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
  • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
  • A01K67/027—New or modified breeds of vertebrates
  • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
  • A01K67/0276—Knock-out vertebrates
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  • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
  • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
  • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
  • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
  • C07K14/4702—Regulators; Modulating activity
  • C07K14/4703—Inhibitors; Suppressors
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
  • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
  • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
  • A01K2217/00—Genetically modified animals
  • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
  • A01K2217/00—Genetically modified animals
  • A01K2217/07—Animals genetically altered by homologous recombination
  • A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
  • A01K2217/00—Genetically modified animals
  • A01K2217/07—Animals genetically altered by homologous recombination
  • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
  • A01K2227/00—Animals characterised by species
  • A01K2227/10—Mammal
  • A01K2227/105—Murine
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
  • A01K2267/00—Animals characterised by purpose
  • A01K2267/03—Animal model, e.g. for test or diseases
  • A01K2267/0331—Animal model for proliferative diseases
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
  • A01K2267/00—Animals characterised by purpose
  • A01K2267/03—Animal model, e.g. for test or diseases
  • A01K2267/0393—Animal model comprising a reporter system for screening tests
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N2800/00—Nucleic acids vectors
  • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N2830/00—Vector systems having a special element relevant for transcription
  • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination

Definitions

• This invention relates to a multi-product fuel dispenser and, more particularly, to such a dispenser that feeds more than one product through an ultrasonic metering device and a single hose and nozzle.
• multi-product fuel dispensers or pumps each for dispensing a plurality of different grades, or octane levels, of gasoline products at each fueling station.
• three different products are provided per fueling station, namely a high octane fuel, a medium octane fuel and a low octane fuel.
• multi-product dispensers had a separate hose for each product. Now, many such dispensers use the same hose and nozzle to dispense all products.
• dispensers typically include a separate flow path for each product from its reservoir product tank which stores the fuel, to the outlet nozzle which introduces the fuel into the consumer's automobile. These systems therefore require the duplication of the components disposed between the tank and the nozzle for each fuel product, including the flow meter. In this manner, however, no contamination of the octane level of the products can occur.
• dispensers of the prior art avoid contamination of fuel being dispensed at a particular time, with fuel from a previous use that would otherwise remain in the system at the termination of the last dispensing cycle.
• Spalding U.S. Patent No. 5,332,011, a patent assigned to the assignee of the present invention, discloses such a dispenser, in which three nozzles, fuel hoses and flow meters, each for a different grade of gasoline, are combined in a single dispenser.
• different grades of fuel from three different storage sources can be delivered through a common meter and then dispensed through a dedicated hose and nozzle for each fuel grade.
• a specific valving arrangement controls the flow of a specific fuel grade through the meter and to the dedicated hose and nozzle.
• different grades of fuel from three different storage sources can be delivered through a common meter and then selectively dispensed through a single hose and nozzle. In this arrangement, valving selectively directs a specific fuel grade to the common meter and the meter is connected to the single hose and nozzle.
• fuel delivery of various grades, through a single hose and nozzle is accomplished from two different grades of fuel (i.e., highest octane and lowest octane) stored separately.
• a specific valving arrangement controls the delivery of the selected fuel grade.
• the separately stored fuels may be blended to deliver one or more intermediate grades of fuel. This may be accomplished by proportional blending or fixed ratio blending.
• proportional blending various intermediate grades are a selectively blended mixture of some proportion of the high and low octane fuels.
• fixed ratio blending a single intermediate grade is produced including a fixed percentage of the high and low octane fuels.
• blending dispensers there are two separate sets of hydraulics. One set is for controlling the low octane product input and another set is for controlling the high octane product input.
• the low and high octane hydraulic systems each contain a proportional flow control valve.
• the blend ratio programmed into the dispenser's computer determines the percentage or proportion of high product to be dispensed.
• the proportion or percentage of high product is 0%.
• the high grade product is selected, the percentage of high product is 100%.
• a blended grade is selected, a percentage of high product (less than 100%) is mixed with the remaining percentage of low product, and the combined total (100%) determines the octane rating of the blended grade.
• the computer signals the solenoid drive board which in turn controls the proportional flow control valves.
• Each proportional flow control valve continuously opens or closes, as directed by the solenoid drive board, to maintain the desired blend ratio and the maximum allowable flow rate.
• Some gasoline station operators would prefer to have a dispenser hose provided with a greater than normal length.
• the normal hose length provided is about 12 feet.
• the volume of fuel retained in a 12 foot length of hose and the volume of fuel in the flow meter approximates the allowable 0.3 gallon contamination factor. Therefore, extending the hose length to, for example, 13 feet may cause the system to exceed the 0.3 gallons of allowable contamination due to the increased volume of the extended length of the hose.
• the 0.3 gallon contamination factor is generally not permitted. In fact, only the minimal nozzle volume contamination is permitted. Therefore, separate nozzles and hoses are required for each grade of fuel product.
• multi-product fuel dispensers have been developed that comprise tri-axial fuel hoses having three concentric passages within a single hose that lead to a single nozzle.
• Such devices simplify operation for the consumer as there is only a single nozzle, but they do not alleviate the need for separate flow meters for each product or improve the maintenance and repair costs.
• Such devices might actually increase the cost of the dispenser due to the complexity of the tri-axial hoses.
• the present meters include a mechanical positive displacement meter using technology which is over 50 years old. This meter includes over 100 parts, is cumbersome, not service friendly, and not easily interfaced with modern microprocessor based control systems.
• a multiple compartment hose may include separate compartments for delivering three grades of fuel and a fourth compartment for vapor recovery in a non-blending system. Therefore, what is needed is an economically feasible meter of smaller volume, i.e., substantially less than 0.1 gallons, able to operate within a nozzle in combination with a three compartment hose and a blend valve in the nozzle at the system flow rate of 10 gpm, reliable due to few or no moving parts, and capable of almost infinite life.
• a multi-product fuel dispensing system for dispensing a plurality of grades of fuel stored in a plurality of reservoir tanks.
• a fuel delivery line is connected to each reservoir tank.
• a fuel delivery conduit is connected to receive fuel from each fuel delivery line.
• the conduit includes at least two flow channels, one for conveying a first grade of fuel and another for conveying a second grade of fuel.
• a nozzle connected to the conduit includes a blend valve for each flow channel. The nozzle can deliver the first grade of fuel, the second grade of fuel or a third grade of fuel comprising a blend of the first and second grades of fuel.
• An ultrasonic flow meter is mounted in the nozzle to measure the flow of the fuel.
• a principle advantage of this embodiment is that there is little or no contamination which can only occur between the end of the hose and the blend valve in the nozzle. Hose length is no longer an issue related to the contamination factor. Products are kept separate using a partitioned hose. The final product may be blended and/or measured in the nozzle. Therefore, in the U.S. where some contamination is allowed, the system permits added hose length without contamination consequences. In Europe, or anywhere there is no contamination tolerance, this system presents a means for providing a single dispenser, single meter system.
• Fig. 1 is a schematic view illustrating an embodiment of an ultrasonic meter using the sing-around measurement technique.
• Fig. 2 is a schematic view illustrating an embodiment of a fuel dispensing system.
• Fig. 2A is a schematic view illustrating an alternative embodiment of a fuel dispensing system.
• Fig. 3 is a cross-sectional view illustrating an embodiment of a multi- compartment hose.
• Fig. 3A is a cross-sectional view illustrating an embodiment of an alternative multi-compartment hose.
• Fig. 4 is a cross-sectional view illustrating another embodiment of a multi- compartment hose.
• Fig. 5 is a diagrammatic view illustrating an embodiment of a fuel dispenser, dispensing fuel to a vehicle.
• Fig. 6 is a side view, partially cut-away, illustrating an embodiment of a fuel dispensing nozzle.
• Fig. 7 is a side view illustrating another embodiment of a fuel dispensing nozzle.
• Fig. 8 is a side view illustrating a further embodiment of a fuel dispensing nozzle. Detailed Description of the Preferred Embodiment
• Fig. 1 generally illustrates the principle of sing-around type measurement.
• a fluid having the velocity v flows in a tube 1 from the left to the right through an ultrasonic flow meter 5.
• an ultrasonic transducer 2 and 3 respectively, which forms an angle « with the longitudinal axis of the tube 1.
• the transducers 2, 3 are interconnected by means of a sing-around electronics, which are represented in the form of a box 4.
• the velocity v of the fluid is measured in the tube 1.
• a sing-around loop is first established in the one direction by the sing-around electronics 4 exciting the transducer 2 to transmit an ultrasonic pulse, which is received by the transducer 3 after passing through the fluid in the tube 1.
• the sing-around electronics 4 excite the transducer 2 to transmit a new ultrasonic pulse.
• the thus-established sing-around loop is maintained for a predetermined number of turns. Then, this procedure is repeated in the downstream direction.
• the sing-around loop will oscillate with a certain period, which is referred to as the sing-around period and which depends on the sound velocity in the fluid between the transducers, the distance between the transducers, and the fluid velocity v.
• the sing-around period in the downstream directions is measured and the sing-around period in the upstream direction is measured. If the distance between the transducers and the angle « between the respective transducers and the tube are known, and if the sing- around periods are measured, the fluid velocity v can thus be calculated and may be used for determining e.g. the flow rate of mass in the tube 1. With the aid of the sing-around periods, the sound velocity in the fluid may be calculated. In actual practice, the sing-around periods are determined by measuring the time it takes for the ultrasonic pulses to do the predetermined number of turns in the sing-around loops, and dividing it by that predetermined number. When calculating the fluid velocity and the sound velocity, a time correction for the delays in the electronics is made. Referring now to Fig.
• a multi-product fuel dispenser of the present invention is shown schematically and generally referred to with reference numeral 30.
• the dispenser 30 receives fuel from a plurality of underground fuel reservoir tanks 32a, 32b, each of which stores a different grade of fuel such as high and low octane.
• separate fuel delivery lines 34a, 34b pass the fuel from the reservoir tanks 32a, 32b into the dispenser 30 under the control of flow control valves 36a, 36b.
• the fuel delivery lines 34a, 34b attach via an outlet casting 42 to a multi-compartment hose 44 and remain separated in the hose until being blended at a nozzle 46.
• FIG. 2A an alternative non-blending multiproduct fuel dispenser of the present invention is shown schematically and generally referred to with reference numeral 130.
• the dispenser 130 receives fuel from a plurality of underground fuel reservoir tanks 132a, 132b, and 132c each of which stores a different grade of fuel such as high, medium and low octane. Also, separate fuel delivery lines 134a, 134b, and 134c pass the fuel from the reservoir tanks 132a, 132b, 132c, into the dispenser 130 under the control of flow control valves 136, 136b and 136c.
• the fuel delivery lines 134a, 134b, 134c attach via an outlet casting 142 to a multi-compartment hose 144 and remain separated in the hose until being individually dispensed at a nozzle 146.
• the operation of dispenser 30 includes the customer pre-selecting a desired grade of fuel from a product selection panel 48 by pressing an appropriate one of the selection buttons, 48a, 48b or 48c.
• Blend valve functions to deliver the selected fuel which may be either the high octane fuel, the low octane fuel or a blend of the high and low octane fuels thus producing a fuel product having an octane rating between the high and low octane fuel products, respectively.
• Hose 44 Fig. 3
• Hose 44 Fig. 3
• Hose 44 is partitioned and includes a first conduit 50 for conducting a first octane product from fuel delivery line 34a to nozzle 46.
• a second conduit 52 for conducting a second octane product, different from the first octane product, from fuel delivery line 34b to nozzle 46.
• a third conduit 54 in hose 44 provides for vapor recovery.
• a multi-compartment hose 44a includes a first conduit 50a, a second conduit 52a and a third conduit 54a corresponding to conduits 50, 52 and 54, respectively.
• hose 144, Fig. 3A is provided for connection to the non- blending multiproduct fuel dispenser 130 of Fig 2A.
• Hose 144, Fig. 3A is partitioned and includes a first conduit 150 for conducting a first octane product from fuel delivery line 134a to nozzle 146.
• hose 144 Also included in hose 144 is a second conduit 152 for conducting a second octaine product from fuel delivery line 134b to nozzle 146.
• a third conduit 154 in hose 144 conducts a third octaine product, from fuel delivery line 134c to nozzle 146.
• a fourth conduit 155 in hose 144 provides for vapor recovery.
• outlet casting 42 is attached to dispenser unit 30.
• Hose 44 extends from outlet casting 42 to nozzle 46.
• Product selection is made at product selection panel 48, and fuel is delivered to vehicle 62 via nozzle 46.
• the nozzle 46, Fig. 6, includes an inlet end 46a and a delivery end 46b.
• the nozzle 46 is hand-held in the usual manner and manual operation of an actuating trigger 60 after fuel grade selection is made, controls fuel delivery from nozzle 46 to the vehicle 62, Fig. 5, or the like.
• nozzle 46 includes a vapor recovery conduit 64 therein which extends from adjacent the nozzle delivery end 46b to the nozzle inlet end 46a. In this manner, vapor recovery conduit 64 interconnects with vapor recovery conduit 54 of hose 44.
• First conduit 50 delivers the first octane product to a first blend valve 70a in nozzle 46.
• Second conduit 52 delivers the second octane product to a second blend valve 70b in nozzle 46.
• the blend valves 70a and 70b function in the usual manner, depending on product selection, and deliver either the first product, the second product or a third product comprising a blend of the first and second products.
• the selected product exits to a conduit 70c and enters tube 1 of ultrasonic flow meter 5 and measurement is accomplished by the transducers 2 and 3 as described above.
• the electronics in box 4, Fig. 1 communicate from the nozzle 46 to dispenser 30, Figs. 2 and 5 in a known manner and may be hard wired via hose 44.
• a modified attachment 80, Fig. 7, to hose 44 may house blend valves 70a, 70b and ultrasonic meter 5.
• the attachment 80 may be a well-known breakaway attachment of the type used to limit damage to a fuel dispenser when a customer forgets to remove nozzle 46 from vehicle 62, Fig. 5, and drives off with the nozzle 46 still engaged with the vehicle. In this manner, the nozzle 46, may break free of hose 44 and attachment 80 thus permitting the blend valves 70a, 70b, Fig. 7, and ultrasonic meter 5 to remain with hose 44 in attachment 80.
• a modified swivel connection 90 may house blend valves 70a, 70b, and ultrasonic meter 5.
• the swivel connection 90 provides a swivel device interconnecting hose 44 and nozzle 46 so as to provide improved freedom of movement of nozzle 46 relative to hose 44, in the event that hose 44 becomes twisted from repeated use.
• Swivel connection 90 may be used with or without a breakaway attachment 80 as described above. It should be noted that in connection with the above-described blend systems used with the dispenser of Fig. 2, blend valves 70a and 70b may not be required because flow control valves 36a and 36b may also be provided to function as blend valves.
• the principal advantages of these embodiments include little or no contamination which can occur between the end of the hose and the blend valve in the nozzle.
• Hose length is no longer an issue related to the contamination factor.
• Products are kept separate using a partitioned hose.
• the delivered product may be blended and/or measured in the nozzle or immediately adjacent the nozzle.
• one embodiment provides a multi-product fuel dispensing system which includes a plurality of reservoir tanks, each tank having a specific grade of fuel stored therein, and each grade being different from each other grade.
• Each reservoir tank is connected to a respective fuel delivery line.
• a fuel delivery conduit which has at least two flow channels, is connected to receive fuel from each fuel delivery line.
• a nozzle is connected to receive fuel from the fuel delivery conduit, and a blend valve for each flow channel is mounted in the nozzle for selectively delivering each specific grade of fuel from the tanks or for delivering a blend of the specific grades of fuel.
• An ultrasonic flow meter is mounted adjacent the blend valve for measuring the flow of the fuel through the nozzle.
• a multi-product fuel dispensing system including first and second reservoir tanks each storing, respectively, a first and a second grade of fuel.
• a first fuel delivery line is connected to the first tank, and a second fuel delivery line is connected to the second tank.
• Each fuel delivery line includes a respective flow control valve.
• a fuel delivery conduit includes at least two flow channels connected to receive fuel from the fuel delivery lines such that one of the flow channels receives fuel from the first fuel delivery line and the other of the flow channels receives fuel from the second fuel delivery line.
• a nozzle is connected to receive fuel from the fuel delivery conduit.
• a blend valve for each flow channel is mounted in the nozzle for selectively delivering the first grade of fuel, the second grade of fuel, or a blend including the first and second grades of fuel.
• An ultrasonic flow meter is mounted adjacent the blend valve for measuring the flow of fuel delivered through the nozzle.
• a further embodiment provides a method of measuring multi-grade fuel flow by connecting a multi-conduit fuel delivery line to separately receive a first and a second grade of fuel from a first and a second fuel source, respectively.
• a nozzle is attached to the multi-conduit fuel delivery line to separately receive the first and second grades of fuel, which are passed through a respective blend valve prior to delivering a pre-selected grade of fuel from the nozzle.
• the pre-selected grade of fuel is also passed through an ultrasonic flow meter prior to delivering the preselected fuel from the nozzle.

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