EP1032681A1 - Chimäres gen unter kontrolle eines lichtabhängigen promotors welches toleranz gegenüber hppd-hemmstoffen vermittelt - Google Patents

Chimäres gen unter kontrolle eines lichtabhängigen promotors welches toleranz gegenüber hppd-hemmstoffen vermittelt

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Publication number
EP1032681A1
EP1032681A1 EP98954565A EP98954565A EP1032681A1 EP 1032681 A1 EP1032681 A1 EP 1032681A1 EP 98954565 A EP98954565 A EP 98954565A EP 98954565 A EP98954565 A EP 98954565A EP 1032681 A1 EP1032681 A1 EP 1032681A1
Authority
EP
European Patent Office
Prior art keywords
chimeric gene
plants
promoter
hppd
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP98954565A
Other languages
English (en)
French (fr)
Inventor
Luc Reygnier
Alain Sailland
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer CropScience SA
Original Assignee
Aventis CropScience SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aventis CropScience SA filed Critical Aventis CropScience SA
Publication of EP1032681A1 publication Critical patent/EP1032681A1/de
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance

Definitions

  • the present invention relates to a chimeric gene having a light-dependent promoter operably linked to a gene coding for a HPPD (hydroxy phenyl pyruvate dioxygenase), conferring improved tolerance to herbicides inhibitors of 1 ⁇ PPD to a normally sensitive plant cell and plant .
  • HPPD hydroxy phenyl pyruvate dioxygenase
  • the invention also relates to plant cells and palntes transformed with the chimeric gene according to the invention, a method for transforming plant cells and plants and a method for cultivating transformed plants in which an herbicide inhibitor of HPPD is applied to eliminate weeds.
  • the herbicides targeting HPPD are in particular isoxazoles (EP 418 175, EP 470 856, EP 487 352, EP 527 036, EP 560 482, EP 682 659, US 5 424 276) in particular isoxaflutole, a selective herbicide corn, diketonitriles (EP 496 630, EP 496 631), in particular 2-cyano-3-cyclopropyl-1- (2-S ⁇ 2 CH3-4-CF3 phenyl) propane- 1, 3 -dione and 2- cyano-3-cyclopropyl-l- (2-S ⁇ 2 CH3 ⁇ 4-2.3 CI2 phenyl) propane- 1, 3-dione, triketones (EP 625 505, EP 625 508, US 5,506,195), in particular sulcotrione or again the pyrazolinates.
  • isoxazoles EP 418 175, EP 470 856, EP 487 352, EP 527 036, EP 560 482, EP 682 659, US 5 424 27
  • LD promoter lightly dependent promoter
  • the present invention therefore firstly relates to a chimeric gene comprising at least one elementary chimeric gene containing, in the direction of transcription, 5 'regulatory elements necessary for its transcription in plants, at least one heterologous coding part. comprising a coding sequence coding for an enzyme conferring on plants tolerance to herbicides which inhibit HPPD and at least one terminator or polyadenylation regulatory sequence, the 5 'regulatory elements ensuring the transcription of the elementary chimeric gene at the tissue level chlorophylliens, the said 5 'regulatory elements preferably comprising at least one promoter regulatory sequence of the LD promoter type.
  • LD promoter any gene promoter coding for peptides whose transcription is induced by light which is functional as a promoter in plant cells.
  • LD promoters can be of bacterial, viral or vegetable origin. Such promoters are in particular described by Terzaghi & coll. (Light-regulated transcription, Annu. Rev. Plant Physiol. Plant Mol. Biol, 1995, 46: 445-474) the content of which is incorporated herein by reference.
  • LD promoter useful according to the invention, there will be mentioned more particularly the promoter of a gene for the small subunit of plant ribulose-biscarboxylase (rbcs), of the protein "light-harvesting chlorophyll a / b binding" (LHCP) , plastocyanine (pet E) and phenylalanine ammonia lyase (pal).
  • rbcs ribulose-biscarboxylase
  • LHCP light-harvesting chlorophyll a / b binding
  • plastocyanine plastocyanine
  • pal phenylalanine ammonia lyase
  • a promoter regulatory sequence which promotes the overexpression of the coding sequence in chlorophyllian tissues, such as for example, that comprising at least one functional fragment of the promoter of the small rbcs subunit (SSU) d 'a plant, more particularly isolated from Helianthus annuus as described in US patent 5,559,024.
  • SSU small rbcs subunit
  • the sequence of the SSU promoter of Helianthus annuus comprises the DNA sequence represented by the sequence identifier No. 1 (SEQ ID NO: 1) or a sequence homologous to said sequence . More preferably, the sequence of the SSU promoter consists of the DNA sequence represented by the sequence identifier No. 1.
  • homologous a DNA sequence having one or more sequence modifications with respect to the reference DNA sequence described by the sequence identifier No. 1, and reproducing the function of this sequence. These modifications can be obtained according to the usual mutation techniques, or alternatively by choosing the synthetic oligonucleotides which can be used in the preparation of said sequence by hybridization.
  • the degree of homology will be at least 70% relative to the reference sequence, preferably at least 80%, more preferably at least 90%.
  • plant cell any cell derived from a monocotyledonous plant and which may constitute undifferentiated tissues such as calluses, differentiated tissues such as embryos, parts of monocotyledonous plants, monocotyledonous plants or seeds.
  • plant means any differentiated multicellular organism capable of photosynthesis, more particularly monocotyledonous or dicotyledonous plants, preferably from cultures intended or not intended for animal or human food, such as, for example, wheat, barley, oats, rice, corn, sorghum, sugarcane, soybeans, rapeseed, cotton, tobacco, beetroot or vegetable or flower crops.
  • promoter regulatory sequence of the LD type other regulatory sequences, which are located between the promoter and the coding sequence, such as enhancer trancription activators, as for example the translation activator of the tobacco etch virus (VTE) described in the article by Carrington and Freed, 1990; J. Virol.
  • enhancer trancription activators as for example the translation activator of the tobacco etch virus (VTE) described in the article by Carrington and Freed, 1990; J. Virol.
  • sequences coding for transit peptides either single or double, and in this case optionally separated by an intermediate sequence, that is to say comprising, in the direction of transcription, a sequence coding for a transit peptide of a plant gene coding for an enzyme with plastid location, a sequence part of the mature N terminal part of a plant gene coding for an enzyme with localization plastid, then a sequence coding for a second transit peptide of a plant gene coding for a plastid localized enzyme, consisting of a sequence part of the mature N terminal part of a plant gene coding for a plastid localized enzyme , as described in patent application EP 508 909.
  • HPPD As coding sequence for an enzyme conferring on plants tolerance to herbicides which inhibit HPPD, it is possible in particular to use all those known to confer plant tolerance on certain inhibitors of HPPD such as the sequences coding for a HPPD described in patent application WO 96/38567 and in patent application FR 9714264 filed on November 7, 1997.
  • This HPPD can be of any kind.
  • this sequence can be of bacterial origin, such as in particular the genus Pseudomonas or also of vegetable origin, such as in particular of monocotyledonous or dicotyledone plant, in particular Arabidopsis or umbelliferae such as for example carrot (Daucus carota). It can be native or wild or possibly mutated while fundamentally retaining a herbicidal tolerance property against HPPD inhibitors, such as herbicides of the isoxazole family or that of the triketones or pyrazinolates.
  • HPPD inhibitors such as herbicides of the isoxazole family or that of the triketones or pyrazinolates.
  • any corresponding sequence of bacterial origin such as for example the terminator nos dAgrobacterium tumefaciens, or of plant origin, such as for example a histone terminator as described in the application, can be used.
  • European EP 633,317 As terminating or polyadenylation regulatory sequence, any corresponding sequence of bacterial origin, such as for example the terminator nos dAgrobacterium tumefaciens, or of plant origin, such as for example a histone terminator as described in the application, can be used.
  • European EP 633,317 As terminating or polyadenylation regulatory sequence, any corresponding sequence of bacterial origin, such as for example the terminator nos dAgrobacterium tumefaciens, or of plant origin, such as for example a histone terminator as described in the application.
  • the present invention also relates to a cloning or expression vector for the transformation of a plant cell or a monocotyledonous or dicotyledonous plant.
  • the vector according to the invention comprises, in addition to the above chimeric gene, at least one origin of replication.
  • This vector can consist of a plasmid, a cosmid, a bacteriophage or a virus, transformed by the introduction of the chimeric gene according to the invention.
  • Such vectors for transforming plant cells and monocotyledonous plants are well known to those skilled in the art and widely described in the literature.
  • the vector for transforming plant cells or plants according to the invention is a plasmid.
  • the subject of the invention is also a process for transforming plant cells by integration of at least one nucleic acid fragment or a chimeric gene as defined above, a transformation which can be obtained by any suitable known means with the vector according to the invention.
  • a series of methods involves bombarding cells or protoplasts with particles to which the DNA sequences are attached. Another series of methods consists in using a chimeric gene as a means of transfer into the plant. inserted into a Ti d grobacterium tumefaciens or Ri plasmid from Agrobacterium rhizogenes. Other methods can be used such as micro-injection or electroporation, or even direct precipitation using PEG.
  • Another subject of the present invention is plant cells or plants, transformed tolerant to herbicides targeting HPPD and containing at least one chimeric gene according to the invention defined above.
  • the present invention also relates to plants containing transformed cells, in particular plants regenerated from transformed cells.
  • the regeneration is obtained by any suitable process which depends on the nature of the species.
  • the present invention also relates to the transformed plants resulting from the culture and / or the crossing of the regenerated plants above, as well as the seeds of transformed plants.
  • the present invention also relates to a method of controlling weeds in a surface of a field comprising seeds or plants transformed with the chimeric gene according to the invention, which method consists in applying in said field surface a toxic dose for the said weeds of a herbicide targeting HPPD, without however substantially affecting the seeds or plants transformed with the said chimeric gene according to the invention.
  • the present invention also relates to a process for cultivating plants transformed according to the invention with a chimeric gene according to the invention, which process consists in planting the seeds of said transformed plants in a surface of a field suitable for the cultivation of said plants, to apply to said surface of said field a toxic dose for weeds of a herbicide targeting HPPD defined above in the presence of weeds, without substantially affecting said seeds or said plants then harvest the cultivated plants when they reach the desired maturity and possibly separate the seeds from the harvested plants.
  • the application of the herbicide targeting HPPD can be carried out according to the invention, both in pre-planting, pre-emergence and post-emergence of the crop.
  • herbicide within the meaning of the present invention is meant a herbicidal active material alone or associated with an additive which modifies its effectiveness such as for example an agent increasing activity (synergist) or limiting activity (in English safener).
  • an agent increasing activity for example an agent increasing activity (synergist) or limiting activity (in English safener).
  • limiting activity in English safener.
  • the above herbicides are associated in a known manner with the adjuvants of formulations usually used in agrochemistry. The various aspects of the invention will be better understood with the aid of the experimental examples below.
  • Example 1 Construction of a chimeric gene with an HPPD sequence and an LD promoter. To confer plant tolerance to herbicides that inhibit HPPD, a chimeric gene called pRPA-RD-2005 is constructed:
  • HPPD HPPD will then be localized in the chloroplast and the gene is mainly expressed in chlorophyll tissues.
  • PRPA-RD-2005 is a binary vector of pRPA-BL-150A ⁇ 2 type (EP 508 909) containing an HPPD expression cassette: promoter of the small subunit of ribulose discarboxylase-OTP-HPPD gene-terminator our
  • pRPA-RD-2004 - pRD-207 is a pBluescript SK (-) (stratagene catalog # 21 2206) containing the gene for nopaline synthase (terminator nos).
  • PRD-207 is used as the basic vector for the construction of pRPA-RD-2004
  • - pRD-208 contains the OTP / HPPD cassette: nos. It is obtained from the plasmid pRPA S, described in WO 96/38567, digested with Xbal / ClaI, treatment with polymerase pfu type. The cassette is introduced into the open pRD-207 by a Sali digestion, klenow treatment.
  • the promoter of the small subunit of the ribulose biscarboxylase from Helianthus annuus comes from the plasmid pRD-127 described in WO 96/38567 digested Ncol / Xbal is introduced into pRD-208. This construction constitutes pRPA-RD-2004
  • Example 2 Processing of PBD6 industrial tobacco.
  • Transformation The vector is introduced into the non-oncogenic strain of Agrobacterium EHA 101 or LBA 4404 (Hood et al, 1987) carrying the cosmid pTVK 291 (Komari et al, 1986). The transformation technique is based on the procedure of Horsh R. et al. (1985) Science, 227, 1229-1231.
  • Regeneration The regeneration of PBD6 tobacco (from SEITA France) from leaf explants is carried out on a Murashige and Skoog (MS) base medium comprising 30g / l of sucrose as well as 350 mg / 1 of cefotaxime and 1 mg / 1 diketonitrile derived from isoxaflutole or 10 mg / 1 l- [4- (trifluoromethyl) -2- (methylsulfonyl) phenyl] -2-cyano- 3- (l-methylcyclopropyl) -propan-1, 3- dione, another HPPD inhibitor.
  • MS Murashige and Skoog
  • the leaf explants are taken from plants in the greenhouse or in vitro and transformed using the leaf disc technique (Science 1985, Vol 227, p.1229-1231) in three successive stages: the first includes the induction of shoots on a medium MS supplemented with 30g / l of sucrose containing 0.05mg / l of naphthylacetic acid (ANA) and 2 mg / 1 of benzylaminopurine (BAP) for 15 days and 1 mg / 1 of isoxaflutole.
  • ANA naphthylacetic acid
  • BAP benzylaminopurine
  • the green shoots formed during this stage are then developed by culture on an MS medium supplemented with 30 g / 1 of sucrose and 1 mg / 1 of isoxaflutole or 10 mg / 1 of 1- [4- (trifluoromethyl) -2 - (methylsulfonyl) phenyl] -2-cyano-3- (1-methylcyclopropyl) -propan 1,3-dione but containing no hormone, for 10 days.
  • the transformed tobacco seedlings were acclimated in the greenhouse (60% relative humidity; temperature: 20 ° C at night and 23 ° C during the day) for three weeks then treated with 4- [4- (trifiuoromethyl) -2- (methylsulfonyl) benzoyl] -5-cyclopropylisoxazole (isoxafutole).
  • the control tobacco, unprocessed and treated with isoxafutole at the doses of 400 g / ha develops in about 72 hours chloroses, which intensify to progress to very pronounced necrosis in a week (covering about 80% of the leaves final).
  • the greenhouse tolerance of 2005 tobacco is compared to that of COI 1 tobacco, transformed with the pRPA-V chimeric gene described in patent application WO 96/38567 and that of unprocessed tobacco. Description of tobacco trials.
  • Example 4 The comparative tolerance tests of Example 4 are reproduced in the field for the same tobacco 2005, COI 1 and PBD6.
  • Young plants from seedlings were transplanted individually in miniballs to be transplanted a second time in the field.
  • the post treatment was carried out one week later at doses of isoxaflutole of 0/100/200/300/400/500/600 g / ha.
  • plants transformed with the pRPA-RD-2005 gene comprising the SSU promoter have better tolerance to HPPD inhibitors than those transformed with the pRPA-V gene comprising the double histone promoter combined with TEV "enhancer" of the state of the art.

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Catching Or Destruction (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
EP98954565A 1997-11-17 1998-11-13 Chimäres gen unter kontrolle eines lichtabhängigen promotors welches toleranz gegenüber hppd-hemmstoffen vermittelt Withdrawn EP1032681A1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR9714591 1997-11-17
FR9714591A FR2771104B1 (fr) 1997-11-17 1997-11-17 Gene chimere ayant un promoteur lumiere dependant conferant la tolerance aux inhibiteurs del'hppd
PCT/FR1998/002414 WO1999025842A1 (fr) 1997-11-17 1998-11-13 Gene chimere ayant un promoteur lumiere dependant conferant la tolerance aux inhibiteurs de l'hppd

Publications (1)

Publication Number Publication Date
EP1032681A1 true EP1032681A1 (de) 2000-09-06

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EP98954565A Withdrawn EP1032681A1 (de) 1997-11-17 1998-11-13 Chimäres gen unter kontrolle eines lichtabhängigen promotors welches toleranz gegenüber hppd-hemmstoffen vermittelt

Country Status (11)

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EP (1) EP1032681A1 (de)
AR (1) AR017630A1 (de)
AU (1) AU747634B2 (de)
BR (1) BR9815628A (de)
CA (1) CA2309880A1 (de)
CO (1) CO4950591A1 (de)
FR (1) FR2771104B1 (de)
ID (1) ID21668A (de)
MA (1) MA24818A1 (de)
WO (1) WO1999025842A1 (de)
ZA (1) ZA9810498B (de)

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FR2815969B1 (fr) 2000-10-30 2004-12-10 Aventis Cropscience Sa Plantes tolerantes aux herbicides par contournement de voie metabolique
CN102741415A (zh) 2010-02-02 2012-10-17 拜尔农科股份公司 使用hppd抑制剂作为选择剂的大豆转化
WO2013026740A2 (en) 2011-08-22 2013-02-28 Bayer Cropscience Nv Methods and means to modify a plant genome
AU2014211570A1 (en) 2013-01-29 2015-07-23 The University Court Of The University Of Glasgow Methods and means for increasing stress tolerance and biomass in plants
CA2957921A1 (en) 2014-07-22 2016-01-28 Nmc, Inc. Improved carbon fixation systems in plants and algae
WO2016050512A1 (en) 2014-10-03 2016-04-07 Bayer Cropscience Nv Methods and means for increasing stress tolerance and biomass in plants
US11666012B2 (en) 2016-03-16 2023-06-06 Basf Se Plants comprising wheat G-type cytoplasmic male sterility restorer genes, molecular markers and uses thereof
CA3017995A1 (en) 2016-03-16 2017-09-21 Antje ROHDE Plants comprising wheat g-type cytoplasmic male sterility restorer genes, molecular markers and uses thereof
EP3429335A1 (de) 2016-03-16 2019-01-23 Basf Se Pflanzen mit wiederherstellungsgenen der cytoplasmischen männlichen sterilität (cms) vom typ g von weizen, molekulare marker und verwendungen davon
CN109715811A (zh) 2016-07-18 2019-05-03 巴斯夫欧洲公司 包含小麦g型胞质雄性不育恢复基因、分子标志物的植物及其用途
AU2017300617A1 (en) 2016-07-18 2019-02-07 Basf Se Plants comprising wheat G-type cytoplasmic male sterility restorer genes, molecular markers and uses thereof
US20190225974A1 (en) 2016-09-23 2019-07-25 BASF Agricultural Solutions Seed US LLC Targeted genome optimization in plants
BR112020014168A2 (pt) 2018-01-12 2020-12-08 Basf Se Proteína, ácido nucleico isolado, gene recombinante, vetor, célula hospedeira, planta, parte de planta ou semente de trigo, métodos para produzir, produto de trigo, farinha, farelo integral, amido, grânulos de amido ou farelo de trigo e métodos para identificar e/ou selecionar uma planta de trigo
CA3099621A1 (en) 2018-05-25 2019-11-28 Basf Se Plants comprising wheat g-type cytoplasmic male sterility restorer genes and uses thereof
EA202092647A1 (ru) 2018-05-25 2021-07-08 Басф Се Растения, содержащие гены-восстановители для цитоплазматической мужской стерильности g-типа пшеницы, и их применение
WO2019224359A1 (en) 2018-05-25 2019-11-28 Basf Se Plants comprising wheat g-type cytoplasmic male sterility restorer genes and uses thereof
WO2019234231A1 (en) 2018-06-08 2019-12-12 Basf Se Plants comprising wheat g-type cytoplasmic male sterility restorer genes and uses thereof
WO2023118541A1 (en) 2021-12-22 2023-06-29 Basf Se Regulatory nucleic acid molecules for modifying gene expression in cereal plants

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US6087563A (en) * 1996-01-29 2000-07-11 Arizona Board Of Regents On Behalf Of The University Of Arizona Cloned arabidopsis p-hydroxyphenyl pyruvic acid dioxygenase DNA
FR2751347B1 (fr) * 1996-07-16 2001-12-07 Rhone Poulenc Agrochimie Gene chimere a plusieurs genes de tolerance herbicide, cellule vegetale et plante tolerantes a plusieurs herbicides

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Publication number Publication date
AU747634B2 (en) 2002-05-16
MA24818A1 (fr) 1999-12-31
FR2771104B1 (fr) 2000-12-08
ZA9810498B (en) 1999-05-24
ID21668A (id) 1999-07-08
FR2771104A1 (fr) 1999-05-21
WO1999025842A1 (fr) 1999-05-27
AU1162899A (en) 1999-06-07
AR017630A1 (es) 2001-09-12
CA2309880A1 (fr) 1999-05-27
BR9815628A (pt) 2000-10-24
CO4950591A1 (es) 2000-09-01

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