WO1999025842A1 - Gene chimere ayant un promoteur lumiere dependant conferant la tolerance aux inhibiteurs de l'hppd - Google Patents
Gene chimere ayant un promoteur lumiere dependant conferant la tolerance aux inhibiteurs de l'hppd Download PDFInfo
- Publication number
- WO1999025842A1 WO1999025842A1 PCT/FR1998/002414 FR9802414W WO9925842A1 WO 1999025842 A1 WO1999025842 A1 WO 1999025842A1 FR 9802414 W FR9802414 W FR 9802414W WO 9925842 A1 WO9925842 A1 WO 9925842A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- chimeric gene
- plants
- promoter
- hppd
- sequence
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 53
- 239000003112 inhibitor Substances 0.000 title abstract description 11
- 230000001419 dependent effect Effects 0.000 title description 4
- 241000196324 Embryophyta Species 0.000 claims abstract description 99
- 239000004009 herbicide Substances 0.000 claims abstract description 26
- 238000000034 method Methods 0.000 claims abstract description 23
- 230000001105 regulatory effect Effects 0.000 claims abstract description 20
- 238000013518 transcription Methods 0.000 claims abstract description 12
- 230000035897 transcription Effects 0.000 claims abstract description 12
- 108090000790 Enzymes Proteins 0.000 claims abstract description 10
- 102000004190 Enzymes Human genes 0.000 claims abstract description 10
- 230000009466 transformation Effects 0.000 claims abstract description 8
- 230000008488 polyadenylation Effects 0.000 claims abstract description 4
- 230000001276 controlling effect Effects 0.000 claims abstract description 3
- 230000002363 herbicidal effect Effects 0.000 claims description 12
- 239000013598 vector Substances 0.000 claims description 10
- 230000008685 targeting Effects 0.000 claims description 9
- 108091026890 Coding region Proteins 0.000 claims description 8
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 7
- 239000013612 plasmid Substances 0.000 claims description 6
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 6
- 244000020551 Helianthus annuus Species 0.000 claims description 5
- 235000003222 Helianthus annuus Nutrition 0.000 claims description 5
- 230000001580 bacterial effect Effects 0.000 claims description 5
- 230000001131 transforming effect Effects 0.000 claims description 5
- 108010003581 Ribulose-bisphosphate carboxylase Proteins 0.000 claims description 4
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 claims description 4
- 239000012634 fragment Substances 0.000 claims description 4
- 239000012190 activator Substances 0.000 claims description 3
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 3
- 231100000816 toxic dose Toxicity 0.000 claims description 3
- 108010034715 Light-Harvesting Protein Complexes Proteins 0.000 claims description 2
- 108700023158 Phenylalanine ammonia-lyases Proteins 0.000 claims description 2
- 108090000051 Plastocyanin Proteins 0.000 claims description 2
- 229930002875 chlorophyll Natural products 0.000 claims description 2
- 235000019804 chlorophyll Nutrition 0.000 claims description 2
- 229930002868 chlorophyll a Natural products 0.000 claims description 2
- 229930002869 chlorophyll b Natural products 0.000 claims description 2
- NSMUHPMZFPKNMZ-VBYMZDBQSA-M chlorophyll b Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C=O)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 NSMUHPMZFPKNMZ-VBYMZDBQSA-M 0.000 claims description 2
- 238000010367 cloning Methods 0.000 claims description 2
- 239000013599 cloning vector Substances 0.000 claims description 2
- 239000013604 expression vector Substances 0.000 claims description 2
- 238000003306 harvesting Methods 0.000 claims description 2
- 230000010076 replication Effects 0.000 claims description 2
- 230000003612 virological effect Effects 0.000 claims description 2
- 230000002401 inhibitory effect Effects 0.000 claims 2
- 102000014914 Carrier Proteins Human genes 0.000 claims 1
- 108091008324 binding proteins Proteins 0.000 claims 1
- 238000009331 sowing Methods 0.000 claims 1
- 231100000331 toxic Toxicity 0.000 claims 1
- 230000002588 toxic effect Effects 0.000 claims 1
- 101100339555 Zymoseptoria tritici HPPD gene Proteins 0.000 abstract 2
- 230000002103 transcriptional effect Effects 0.000 abstract 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 26
- 241000208125 Nicotiana Species 0.000 description 25
- 210000004027 cell Anatomy 0.000 description 17
- 210000001519 tissue Anatomy 0.000 description 13
- 230000014509 gene expression Effects 0.000 description 9
- 239000005571 Isoxaflutole Substances 0.000 description 8
- OYIKARCXOQLFHF-UHFFFAOYSA-N isoxaflutole Chemical compound CS(=O)(=O)C1=CC(C(F)(F)F)=CC=C1C(=O)C1=C(C2CC2)ON=C1 OYIKARCXOQLFHF-UHFFFAOYSA-N 0.000 description 8
- 229940088649 isoxaflutole Drugs 0.000 description 8
- 231100000674 Phytotoxicity Toxicity 0.000 description 7
- 238000010276 construction Methods 0.000 description 6
- 108010033040 Histones Proteins 0.000 description 5
- 238000011069 regeneration method Methods 0.000 description 5
- 108700001094 Plant Genes Proteins 0.000 description 4
- 241000723792 Tobacco etch virus Species 0.000 description 4
- 240000008042 Zea mays Species 0.000 description 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 4
- 210000002706 plastid Anatomy 0.000 description 4
- 230000008929 regeneration Effects 0.000 description 4
- 241000589516 Pseudomonas Species 0.000 description 3
- 229930006000 Sucrose Natural products 0.000 description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 3
- 235000005822 corn Nutrition 0.000 description 3
- 239000003623 enhancer Substances 0.000 description 3
- 239000005720 sucrose Substances 0.000 description 3
- 208000024891 symptom Diseases 0.000 description 3
- 235000013311 vegetables Nutrition 0.000 description 3
- 240000002791 Brassica napus Species 0.000 description 2
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 2
- ZAQJHHRNXZUBTE-NQXXGFSBSA-N D-ribulose Chemical compound OC[C@@H](O)[C@@H](O)C(=O)CO ZAQJHHRNXZUBTE-NQXXGFSBSA-N 0.000 description 2
- ZAQJHHRNXZUBTE-UHFFFAOYSA-N D-threo-2-Pentulose Natural products OCC(O)C(O)C(=O)CO ZAQJHHRNXZUBTE-UHFFFAOYSA-N 0.000 description 2
- 244000000626 Daucus carota Species 0.000 description 2
- 235000002767 Daucus carota Nutrition 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- WSMYVTOQOOLQHP-UHFFFAOYSA-N Malondialdehyde Chemical compound O=CCC=O WSMYVTOQOOLQHP-UHFFFAOYSA-N 0.000 description 2
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 description 2
- 244000061176 Nicotiana tabacum Species 0.000 description 2
- 241000589540 Pseudomonas fluorescens Species 0.000 description 2
- 241001233957 eudicotyledons Species 0.000 description 2
- 230000035784 germination Effects 0.000 description 2
- 208000037824 growth disorder Diseases 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000002018 overexpression Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- XUHGTGGPZFJRMF-UHFFFAOYSA-N 1,3-dihydropyrazole-2-carboxylic acid Chemical class OC(=O)N1CC=CN1 XUHGTGGPZFJRMF-UHFFFAOYSA-N 0.000 description 1
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 description 1
- HUTNOYOBQPAKIA-UHFFFAOYSA-N 1h-pyrazin-2-one Chemical class OC1=CN=CC=N1 HUTNOYOBQPAKIA-UHFFFAOYSA-N 0.000 description 1
- ZWSNPZPIJHQHGA-UHFFFAOYSA-N 2-(1-methylcyclopropanecarbonyl)-3-[2-methylsulfonyl-4-(trifluoromethyl)phenyl]-3-oxopropanenitrile Chemical compound C=1C=C(C(F)(F)F)C=C(S(C)(=O)=O)C=1C(=O)C(C#N)C(=O)C1(C)CC1 ZWSNPZPIJHQHGA-UHFFFAOYSA-N 0.000 description 1
- ZTTKDUXKVPEXCG-UHFFFAOYSA-N 2-cyano-3-cyclopropyl-1-(2-mesyl-4-trifluoromethylphenyl)propan-1,3-dione Chemical compound CS(=O)(=O)C1=CC(C(F)(F)F)=CC=C1C(=O)C(C#N)C(=O)C1CC1 ZTTKDUXKVPEXCG-UHFFFAOYSA-N 0.000 description 1
- 241000589158 Agrobacterium Species 0.000 description 1
- 241000589156 Agrobacterium rhizogenes Species 0.000 description 1
- 241000208173 Apiaceae Species 0.000 description 1
- 241000219194 Arabidopsis Species 0.000 description 1
- 241000219195 Arabidopsis thaliana Species 0.000 description 1
- 235000007319 Avena orientalis Nutrition 0.000 description 1
- 244000075850 Avena orientalis Species 0.000 description 1
- 235000021537 Beetroot Nutrition 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 102000016680 Dioxygenases Human genes 0.000 description 1
- 108010028143 Dioxygenases Proteins 0.000 description 1
- 241000219146 Gossypium Species 0.000 description 1
- 206010053759 Growth retardation Diseases 0.000 description 1
- 101150012639 HPPD gene Proteins 0.000 description 1
- 102100034535 Histone H3.1 Human genes 0.000 description 1
- 101001067844 Homo sapiens Histone H3.1 Proteins 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 240000001140 Mimosa pudica Species 0.000 description 1
- 235000016462 Mimosa pudica Nutrition 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 240000007377 Petunia x hybrida Species 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
- 235000007201 Saccharum officinarum Nutrition 0.000 description 1
- 240000006394 Sorghum bicolor Species 0.000 description 1
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 1
- 239000005618 Sulcotrione Substances 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 125000003236 benzoyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C(*)=O 0.000 description 1
- 229960004261 cefotaxime Drugs 0.000 description 1
- AZZMGZXNTDTSME-JUZDKLSSSA-M cefotaxime sodium Chemical compound [Na+].N([C@@H]1C(N2C(=C(COC(C)=O)CS[C@@H]21)C([O-])=O)=O)C(=O)\C(=N/OC)C1=CSC(N)=N1 AZZMGZXNTDTSME-JUZDKLSSSA-M 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 210000003763 chloroplast Anatomy 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 231100000001 growth retardation Toxicity 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 208000006278 hypochromic anemia Diseases 0.000 description 1
- 230000001965 increasing effect Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- CTAPFRYPJLPFDF-UHFFFAOYSA-N isoxazole Chemical compound C=1C=NOC=1 CTAPFRYPJLPFDF-UHFFFAOYSA-N 0.000 description 1
- 150000002545 isoxazoles Chemical class 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 125000004170 methylsulfonyl group Chemical group [H]C([H])([H])S(*)(=O)=O 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000006870 ms-medium Substances 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 230000017074 necrotic cell death Effects 0.000 description 1
- 231100001222 nononcogenic Toxicity 0.000 description 1
- 108010058731 nopaline synthase Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical group 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 210000001938 protoplast Anatomy 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 239000012882 rooting medium Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 235000002639 sodium chloride Nutrition 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- PQTBTIFWAXVEPB-UHFFFAOYSA-N sulcotrione Chemical compound ClC1=CC(S(=O)(=O)C)=CC=C1C(=O)C1C(=O)CCCC1=O PQTBTIFWAXVEPB-UHFFFAOYSA-N 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
Definitions
- the present invention relates to a chimeric gene having a light-dependent promoter operably linked to a gene coding for a HPPD (hydroxy phenyl pyruvate dioxygenase), conferring improved tolerance to herbicides inhibitors of 1 ⁇ PPD to a normally sensitive plant cell and plant .
- HPPD hydroxy phenyl pyruvate dioxygenase
- the invention also relates to plant cells and palntes transformed with the chimeric gene according to the invention, a method for transforming plant cells and plants and a method for cultivating transformed plants in which an herbicide inhibitor of HPPD is applied to eliminate weeds.
- the herbicides targeting HPPD are in particular isoxazoles (EP 418 175, EP 470 856, EP 487 352, EP 527 036, EP 560 482, EP 682 659, US 5 424 276) in particular isoxaflutole, a selective herbicide corn, diketonitriles (EP 496 630, EP 496 631), in particular 2-cyano-3-cyclopropyl-1- (2-S ⁇ 2 CH3-4-CF3 phenyl) propane- 1, 3 -dione and 2- cyano-3-cyclopropyl-l- (2-S ⁇ 2 CH3 ⁇ 4-2.3 CI2 phenyl) propane- 1, 3-dione, triketones (EP 625 505, EP 625 508, US 5,506,195), in particular sulcotrione or again the pyrazolinates.
- isoxazoles EP 418 175, EP 470 856, EP 487 352, EP 527 036, EP 560 482, EP 682 659, US 5 424 27
- LD promoter lightly dependent promoter
- the present invention therefore firstly relates to a chimeric gene comprising at least one elementary chimeric gene containing, in the direction of transcription, 5 'regulatory elements necessary for its transcription in plants, at least one heterologous coding part. comprising a coding sequence coding for an enzyme conferring on plants tolerance to herbicides which inhibit HPPD and at least one terminator or polyadenylation regulatory sequence, the 5 'regulatory elements ensuring the transcription of the elementary chimeric gene at the tissue level chlorophylliens, the said 5 'regulatory elements preferably comprising at least one promoter regulatory sequence of the LD promoter type.
- LD promoter any gene promoter coding for peptides whose transcription is induced by light which is functional as a promoter in plant cells.
- LD promoters can be of bacterial, viral or vegetable origin. Such promoters are in particular described by Terzaghi & coll. (Light-regulated transcription, Annu. Rev. Plant Physiol. Plant Mol. Biol, 1995, 46: 445-474) the content of which is incorporated herein by reference.
- LD promoter useful according to the invention, there will be mentioned more particularly the promoter of a gene for the small subunit of plant ribulose-biscarboxylase (rbcs), of the protein "light-harvesting chlorophyll a / b binding" (LHCP) , plastocyanine (pet E) and phenylalanine ammonia lyase (pal).
- rbcs ribulose-biscarboxylase
- LHCP light-harvesting chlorophyll a / b binding
- plastocyanine plastocyanine
- pal phenylalanine ammonia lyase
- a promoter regulatory sequence which promotes the overexpression of the coding sequence in chlorophyllian tissues, such as for example, that comprising at least one functional fragment of the promoter of the small rbcs subunit (SSU) d 'a plant, more particularly isolated from Helianthus annuus as described in US patent 5,559,024.
- SSU small rbcs subunit
- the sequence of the SSU promoter of Helianthus annuus comprises the DNA sequence represented by the sequence identifier No. 1 (SEQ ID NO: 1) or a sequence homologous to said sequence . More preferably, the sequence of the SSU promoter consists of the DNA sequence represented by the sequence identifier No. 1.
- homologous a DNA sequence having one or more sequence modifications with respect to the reference DNA sequence described by the sequence identifier No. 1, and reproducing the function of this sequence. These modifications can be obtained according to the usual mutation techniques, or alternatively by choosing the synthetic oligonucleotides which can be used in the preparation of said sequence by hybridization.
- the degree of homology will be at least 70% relative to the reference sequence, preferably at least 80%, more preferably at least 90%.
- plant cell any cell derived from a monocotyledonous plant and which may constitute undifferentiated tissues such as calluses, differentiated tissues such as embryos, parts of monocotyledonous plants, monocotyledonous plants or seeds.
- plant means any differentiated multicellular organism capable of photosynthesis, more particularly monocotyledonous or dicotyledonous plants, preferably from cultures intended or not intended for animal or human food, such as, for example, wheat, barley, oats, rice, corn, sorghum, sugarcane, soybeans, rapeseed, cotton, tobacco, beetroot or vegetable or flower crops.
- promoter regulatory sequence of the LD type other regulatory sequences, which are located between the promoter and the coding sequence, such as enhancer trancription activators, as for example the translation activator of the tobacco etch virus (VTE) described in the article by Carrington and Freed, 1990; J. Virol.
- enhancer trancription activators as for example the translation activator of the tobacco etch virus (VTE) described in the article by Carrington and Freed, 1990; J. Virol.
- sequences coding for transit peptides either single or double, and in this case optionally separated by an intermediate sequence, that is to say comprising, in the direction of transcription, a sequence coding for a transit peptide of a plant gene coding for an enzyme with plastid location, a sequence part of the mature N terminal part of a plant gene coding for an enzyme with localization plastid, then a sequence coding for a second transit peptide of a plant gene coding for a plastid localized enzyme, consisting of a sequence part of the mature N terminal part of a plant gene coding for a plastid localized enzyme , as described in patent application EP 508 909.
- HPPD As coding sequence for an enzyme conferring on plants tolerance to herbicides which inhibit HPPD, it is possible in particular to use all those known to confer plant tolerance on certain inhibitors of HPPD such as the sequences coding for a HPPD described in patent application WO 96/38567 and in patent application FR 9714264 filed on November 7, 1997.
- This HPPD can be of any kind.
- this sequence can be of bacterial origin, such as in particular the genus Pseudomonas or also of vegetable origin, such as in particular of monocotyledonous or dicotyledone plant, in particular Arabidopsis or umbelliferae such as for example carrot (Daucus carota). It can be native or wild or possibly mutated while fundamentally retaining a herbicidal tolerance property against HPPD inhibitors, such as herbicides of the isoxazole family or that of the triketones or pyrazinolates.
- HPPD inhibitors such as herbicides of the isoxazole family or that of the triketones or pyrazinolates.
- any corresponding sequence of bacterial origin such as for example the terminator nos dAgrobacterium tumefaciens, or of plant origin, such as for example a histone terminator as described in the application, can be used.
- European EP 633,317 As terminating or polyadenylation regulatory sequence, any corresponding sequence of bacterial origin, such as for example the terminator nos dAgrobacterium tumefaciens, or of plant origin, such as for example a histone terminator as described in the application, can be used.
- European EP 633,317 As terminating or polyadenylation regulatory sequence, any corresponding sequence of bacterial origin, such as for example the terminator nos dAgrobacterium tumefaciens, or of plant origin, such as for example a histone terminator as described in the application.
- the present invention also relates to a cloning or expression vector for the transformation of a plant cell or a monocotyledonous or dicotyledonous plant.
- the vector according to the invention comprises, in addition to the above chimeric gene, at least one origin of replication.
- This vector can consist of a plasmid, a cosmid, a bacteriophage or a virus, transformed by the introduction of the chimeric gene according to the invention.
- Such vectors for transforming plant cells and monocotyledonous plants are well known to those skilled in the art and widely described in the literature.
- the vector for transforming plant cells or plants according to the invention is a plasmid.
- the subject of the invention is also a process for transforming plant cells by integration of at least one nucleic acid fragment or a chimeric gene as defined above, a transformation which can be obtained by any suitable known means with the vector according to the invention.
- a series of methods involves bombarding cells or protoplasts with particles to which the DNA sequences are attached. Another series of methods consists in using a chimeric gene as a means of transfer into the plant. inserted into a Ti d grobacterium tumefaciens or Ri plasmid from Agrobacterium rhizogenes. Other methods can be used such as micro-injection or electroporation, or even direct precipitation using PEG.
- Another subject of the present invention is plant cells or plants, transformed tolerant to herbicides targeting HPPD and containing at least one chimeric gene according to the invention defined above.
- the present invention also relates to plants containing transformed cells, in particular plants regenerated from transformed cells.
- the regeneration is obtained by any suitable process which depends on the nature of the species.
- the present invention also relates to the transformed plants resulting from the culture and / or the crossing of the regenerated plants above, as well as the seeds of transformed plants.
- the present invention also relates to a method of controlling weeds in a surface of a field comprising seeds or plants transformed with the chimeric gene according to the invention, which method consists in applying in said field surface a toxic dose for the said weeds of a herbicide targeting HPPD, without however substantially affecting the seeds or plants transformed with the said chimeric gene according to the invention.
- the present invention also relates to a process for cultivating plants transformed according to the invention with a chimeric gene according to the invention, which process consists in planting the seeds of said transformed plants in a surface of a field suitable for the cultivation of said plants, to apply to said surface of said field a toxic dose for weeds of a herbicide targeting HPPD defined above in the presence of weeds, without substantially affecting said seeds or said plants then harvest the cultivated plants when they reach the desired maturity and possibly separate the seeds from the harvested plants.
- the application of the herbicide targeting HPPD can be carried out according to the invention, both in pre-planting, pre-emergence and post-emergence of the crop.
- herbicide within the meaning of the present invention is meant a herbicidal active material alone or associated with an additive which modifies its effectiveness such as for example an agent increasing activity (synergist) or limiting activity (in English safener).
- an agent increasing activity for example an agent increasing activity (synergist) or limiting activity (in English safener).
- limiting activity in English safener.
- the above herbicides are associated in a known manner with the adjuvants of formulations usually used in agrochemistry. The various aspects of the invention will be better understood with the aid of the experimental examples below.
- Example 1 Construction of a chimeric gene with an HPPD sequence and an LD promoter. To confer plant tolerance to herbicides that inhibit HPPD, a chimeric gene called pRPA-RD-2005 is constructed:
- HPPD HPPD will then be localized in the chloroplast and the gene is mainly expressed in chlorophyll tissues.
- PRPA-RD-2005 is a binary vector of pRPA-BL-150A ⁇ 2 type (EP 508 909) containing an HPPD expression cassette: promoter of the small subunit of ribulose discarboxylase-OTP-HPPD gene-terminator our
- pRPA-RD-2004 - pRD-207 is a pBluescript SK (-) (stratagene catalog # 21 2206) containing the gene for nopaline synthase (terminator nos).
- PRD-207 is used as the basic vector for the construction of pRPA-RD-2004
- - pRD-208 contains the OTP / HPPD cassette: nos. It is obtained from the plasmid pRPA S, described in WO 96/38567, digested with Xbal / ClaI, treatment with polymerase pfu type. The cassette is introduced into the open pRD-207 by a Sali digestion, klenow treatment.
- the promoter of the small subunit of the ribulose biscarboxylase from Helianthus annuus comes from the plasmid pRD-127 described in WO 96/38567 digested Ncol / Xbal is introduced into pRD-208. This construction constitutes pRPA-RD-2004
- Example 2 Processing of PBD6 industrial tobacco.
- Transformation The vector is introduced into the non-oncogenic strain of Agrobacterium EHA 101 or LBA 4404 (Hood et al, 1987) carrying the cosmid pTVK 291 (Komari et al, 1986). The transformation technique is based on the procedure of Horsh R. et al. (1985) Science, 227, 1229-1231.
- Regeneration The regeneration of PBD6 tobacco (from SEITA France) from leaf explants is carried out on a Murashige and Skoog (MS) base medium comprising 30g / l of sucrose as well as 350 mg / 1 of cefotaxime and 1 mg / 1 diketonitrile derived from isoxaflutole or 10 mg / 1 l- [4- (trifluoromethyl) -2- (methylsulfonyl) phenyl] -2-cyano- 3- (l-methylcyclopropyl) -propan-1, 3- dione, another HPPD inhibitor.
- MS Murashige and Skoog
- the leaf explants are taken from plants in the greenhouse or in vitro and transformed using the leaf disc technique (Science 1985, Vol 227, p.1229-1231) in three successive stages: the first includes the induction of shoots on a medium MS supplemented with 30g / l of sucrose containing 0.05mg / l of naphthylacetic acid (ANA) and 2 mg / 1 of benzylaminopurine (BAP) for 15 days and 1 mg / 1 of isoxaflutole.
- ANA naphthylacetic acid
- BAP benzylaminopurine
- the green shoots formed during this stage are then developed by culture on an MS medium supplemented with 30 g / 1 of sucrose and 1 mg / 1 of isoxaflutole or 10 mg / 1 of 1- [4- (trifluoromethyl) -2 - (methylsulfonyl) phenyl] -2-cyano-3- (1-methylcyclopropyl) -propan 1,3-dione but containing no hormone, for 10 days.
- the transformed tobacco seedlings were acclimated in the greenhouse (60% relative humidity; temperature: 20 ° C at night and 23 ° C during the day) for three weeks then treated with 4- [4- (trifiuoromethyl) -2- (methylsulfonyl) benzoyl] -5-cyclopropylisoxazole (isoxafutole).
- the control tobacco, unprocessed and treated with isoxafutole at the doses of 400 g / ha develops in about 72 hours chloroses, which intensify to progress to very pronounced necrosis in a week (covering about 80% of the leaves final).
- the greenhouse tolerance of 2005 tobacco is compared to that of COI 1 tobacco, transformed with the pRPA-V chimeric gene described in patent application WO 96/38567 and that of unprocessed tobacco. Description of tobacco trials.
- Example 4 The comparative tolerance tests of Example 4 are reproduced in the field for the same tobacco 2005, COI 1 and PBD6.
- Young plants from seedlings were transplanted individually in miniballs to be transplanted a second time in the field.
- the post treatment was carried out one week later at doses of isoxaflutole of 0/100/200/300/400/500/600 g / ha.
- plants transformed with the pRPA-RD-2005 gene comprising the SSU promoter have better tolerance to HPPD inhibitors than those transformed with the pRPA-V gene comprising the double histone promoter combined with TEV "enhancer" of the state of the art.
Abstract
Description
Claims
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
BR9815628-4A BR9815628A (pt) | 1997-11-17 | 1998-11-13 | Gene quimérico, vetor, processo de transformação das células vegetais, célula vegetal transformada, planta transformada, sementes, processo de controle das ervas daninhas em uma superfìcie de um campo e processo de cultura das plantas transformadas |
CA002309880A CA2309880A1 (fr) | 1997-11-17 | 1998-11-13 | Gene chimere ayant un promoteur lumiere dependant conferant la tolerance aux inhibiteurs de l'hppd |
AU11628/99A AU747634B2 (en) | 1997-11-17 | 1998-11-13 | Chimera gene having a light dependent promoter providing tolerance to HPPD inhibitors |
EP98954565A EP1032681A1 (fr) | 1997-11-17 | 1998-11-13 | Gene chimere ayant un promoteur lumiere dependant conferant la tolerance aux inhibiteurs de l'hppd |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR97/14591 | 1997-11-17 | ||
FR9714591A FR2771104B1 (fr) | 1997-11-17 | 1997-11-17 | Gene chimere ayant un promoteur lumiere dependant conferant la tolerance aux inhibiteurs del'hppd |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1999025842A1 true WO1999025842A1 (fr) | 1999-05-27 |
Family
ID=9513607
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/FR1998/002414 WO1999025842A1 (fr) | 1997-11-17 | 1998-11-13 | Gene chimere ayant un promoteur lumiere dependant conferant la tolerance aux inhibiteurs de l'hppd |
Country Status (11)
Country | Link |
---|---|
EP (1) | EP1032681A1 (fr) |
AR (1) | AR017630A1 (fr) |
AU (1) | AU747634B2 (fr) |
BR (1) | BR9815628A (fr) |
CA (1) | CA2309880A1 (fr) |
CO (1) | CO4950591A1 (fr) |
FR (1) | FR2771104B1 (fr) |
ID (1) | ID21668A (fr) |
MA (1) | MA24818A1 (fr) |
WO (1) | WO1999025842A1 (fr) |
ZA (1) | ZA9810498B (fr) |
Cited By (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2002036787A2 (fr) | 2000-10-30 | 2002-05-10 | Bayer Cropscience S.A. | Plantes tolerantes aux herbicides par contournement de voie metabolique |
WO2011095460A1 (fr) | 2010-02-02 | 2011-08-11 | Bayer Cropscience Ag | Transformation du soja faisant appel à des inhibiteurs de l'hppd en tant qu'agents de sélection |
WO2013026740A2 (fr) | 2011-08-22 | 2013-02-28 | Bayer Cropscience Nv | Procédés et moyens pour modifier un génome de plante |
WO2014118123A1 (fr) | 2013-01-29 | 2014-08-07 | The University Court Of The University Of Glasgow | Procédés et moyens pour augmenter la tolérance aux contraintes et la biomasse dans des plantes |
WO2016014720A2 (fr) | 2014-07-22 | 2016-01-28 | Nmc, Inc. | Systèmes améliorés de fixation du carbone chez les plantes et les algues |
WO2016050512A1 (fr) | 2014-10-03 | 2016-04-07 | Bayer Cropscience Nv | Procédés et moyens pour augmenter la tolérance au stress et la biomasse chez des plantes |
WO2017158126A1 (fr) | 2016-03-16 | 2017-09-21 | Bayer Cropscience Nv | Plantes comprenant des gènes restaurateurs de la stérilité mâle cytoplasmique de type g du blé, marqueurs moléculaires et leurs utilisations |
WO2017158127A1 (fr) | 2016-03-16 | 2017-09-21 | Bayer Cropscience Nv | Plantes comprenant des gènes restaurateurs de la stérilité mâle cytoplasmique de type g du blé, marqueurs moléculaires et leurs utilisations |
WO2017158128A1 (fr) | 2016-03-16 | 2017-09-21 | Bayer Cropscience Nv | Plantes comprenant des gènes de restauration de la stérilité mâle cytoplasmique de type g du blé, marqueurs moléculaires et leurs utilisations |
WO2018015403A1 (fr) | 2016-07-18 | 2018-01-25 | Bayer Cropscience Nv | Plantes comprenant des gènes de restauration de la stérilité mâle cytoplasmique de type g du blé, marqueurs moléculaires et leurs utilisations |
WO2018015404A1 (fr) | 2016-07-18 | 2018-01-25 | Bayer Cropscience Nv | Plantes comprenant des gènes de restauration de la stérilité mâle cytoplasmique de type g du blé, marqueurs moléculaires et leurs utilisations |
WO2018054911A1 (fr) | 2016-09-23 | 2018-03-29 | Bayer Cropscience Nv | Optimisation ciblée du génome dans des plantes |
WO2019138083A1 (fr) | 2018-01-12 | 2019-07-18 | Basf Se | Gène sous-jacent au nombre de qtl d'épillets par épi de blé sur le chromosome 7a |
WO2019224359A1 (fr) | 2018-05-25 | 2019-11-28 | Basf Se | Plantes comprenant des gènes de restauration de la stérilité mâle cytoplasmique de type g du blé, et leurs utilisations |
WO2019224355A1 (fr) | 2018-05-25 | 2019-11-28 | Basf Se | Plantes comprenant des gènes de restauration de la stérilité mâle cytoplasmique de type g du blé, et leurs utilisations |
WO2019224353A1 (fr) | 2018-05-25 | 2019-11-28 | Basf Se | Plantes comprenant des gènes de restauration de la stérilité mâle cytoplasmique de type g du blé et leurs utilisations |
WO2019234231A1 (fr) | 2018-06-08 | 2019-12-12 | Basf Se | Plantes comprenant des gènes de restauration de la stérilité mâle cytoplasmique de type g du blé et leurs utilisations |
WO2023118541A1 (fr) | 2021-12-22 | 2023-06-29 | Basf Se | Molécules d'acide nucléique régulatrices permettant de modifier l'expression génique de plantes céréalières |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0337899A1 (fr) * | 1988-03-23 | 1989-10-18 | Rhone-Poulenc Agrochimie | Gène chimérique de résistance herbicide |
WO1996038567A2 (fr) * | 1995-06-02 | 1996-12-05 | Rhone-Poulenc Agrochimie | Sequence adn d'un gene de l'hydroxy-phenyl pyruvate dioxygenase et obtention de plantes contenant un gene de l'hydroxy-phenyl pyruvate dioxygenase, tolerantes a certains herbicides |
WO1997027285A1 (fr) * | 1996-01-29 | 1997-07-31 | Arizona Board Of Regents, On Behalf Of University Of Arizona | Dioxygenase vegetale clonee de l'acide p-hydroxyphenylpyruvique |
WO1998002562A2 (fr) * | 1996-07-16 | 1998-01-22 | Rhone-Poulenc Agrochimie | Gene chimere a plusieurs genes de tolerance herbicide, cellule vegetale et plante tolerantes a plusieurs herbicides |
-
1997
- 1997-11-17 FR FR9714591A patent/FR2771104B1/fr not_active Expired - Fee Related
-
1998
- 1998-11-13 AU AU11628/99A patent/AU747634B2/en not_active Ceased
- 1998-11-13 CA CA002309880A patent/CA2309880A1/fr not_active Abandoned
- 1998-11-13 EP EP98954565A patent/EP1032681A1/fr not_active Withdrawn
- 1998-11-13 WO PCT/FR1998/002414 patent/WO1999025842A1/fr not_active Application Discontinuation
- 1998-11-13 BR BR9815628-4A patent/BR9815628A/pt not_active IP Right Cessation
- 1998-11-16 ID IDP981498A patent/ID21668A/id unknown
- 1998-11-16 AR ARP980105795 patent/AR017630A1/es unknown
- 1998-11-17 MA MA25353A patent/MA24818A1/fr unknown
- 1998-11-17 ZA ZA9810498A patent/ZA9810498B/xx unknown
- 1998-11-17 CO CO98067540A patent/CO4950591A1/es unknown
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0337899A1 (fr) * | 1988-03-23 | 1989-10-18 | Rhone-Poulenc Agrochimie | Gène chimérique de résistance herbicide |
WO1996038567A2 (fr) * | 1995-06-02 | 1996-12-05 | Rhone-Poulenc Agrochimie | Sequence adn d'un gene de l'hydroxy-phenyl pyruvate dioxygenase et obtention de plantes contenant un gene de l'hydroxy-phenyl pyruvate dioxygenase, tolerantes a certains herbicides |
WO1997027285A1 (fr) * | 1996-01-29 | 1997-07-31 | Arizona Board Of Regents, On Behalf Of University Of Arizona | Dioxygenase vegetale clonee de l'acide p-hydroxyphenylpyruvique |
WO1998002562A2 (fr) * | 1996-07-16 | 1998-01-22 | Rhone-Poulenc Agrochimie | Gene chimere a plusieurs genes de tolerance herbicide, cellule vegetale et plante tolerantes a plusieurs herbicides |
Cited By (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2002036787A2 (fr) | 2000-10-30 | 2002-05-10 | Bayer Cropscience S.A. | Plantes tolerantes aux herbicides par contournement de voie metabolique |
EP2141240A2 (fr) | 2000-10-30 | 2010-01-06 | Bayer CropScience SA | Plantes tolérantes aux herbicides par contournement de voie métabolique |
WO2011095460A1 (fr) | 2010-02-02 | 2011-08-11 | Bayer Cropscience Ag | Transformation du soja faisant appel à des inhibiteurs de l'hppd en tant qu'agents de sélection |
WO2013026740A2 (fr) | 2011-08-22 | 2013-02-28 | Bayer Cropscience Nv | Procédés et moyens pour modifier un génome de plante |
US9670496B2 (en) | 2011-08-22 | 2017-06-06 | Bayer Cropscience N.V. | Methods and means to modify a plant genome |
US10538774B2 (en) | 2011-08-22 | 2020-01-21 | Basf Agricultural Solutions Seed, Us Llc | Methods and means to modify a plant genome |
WO2014118123A1 (fr) | 2013-01-29 | 2014-08-07 | The University Court Of The University Of Glasgow | Procédés et moyens pour augmenter la tolérance aux contraintes et la biomasse dans des plantes |
WO2016014720A2 (fr) | 2014-07-22 | 2016-01-28 | Nmc, Inc. | Systèmes améliorés de fixation du carbone chez les plantes et les algues |
WO2016050512A1 (fr) | 2014-10-03 | 2016-04-07 | Bayer Cropscience Nv | Procédés et moyens pour augmenter la tolérance au stress et la biomasse chez des plantes |
WO2017158128A1 (fr) | 2016-03-16 | 2017-09-21 | Bayer Cropscience Nv | Plantes comprenant des gènes de restauration de la stérilité mâle cytoplasmique de type g du blé, marqueurs moléculaires et leurs utilisations |
WO2017158127A1 (fr) | 2016-03-16 | 2017-09-21 | Bayer Cropscience Nv | Plantes comprenant des gènes restaurateurs de la stérilité mâle cytoplasmique de type g du blé, marqueurs moléculaires et leurs utilisations |
WO2017158126A1 (fr) | 2016-03-16 | 2017-09-21 | Bayer Cropscience Nv | Plantes comprenant des gènes restaurateurs de la stérilité mâle cytoplasmique de type g du blé, marqueurs moléculaires et leurs utilisations |
WO2018015403A1 (fr) | 2016-07-18 | 2018-01-25 | Bayer Cropscience Nv | Plantes comprenant des gènes de restauration de la stérilité mâle cytoplasmique de type g du blé, marqueurs moléculaires et leurs utilisations |
WO2018015404A1 (fr) | 2016-07-18 | 2018-01-25 | Bayer Cropscience Nv | Plantes comprenant des gènes de restauration de la stérilité mâle cytoplasmique de type g du blé, marqueurs moléculaires et leurs utilisations |
WO2018054911A1 (fr) | 2016-09-23 | 2018-03-29 | Bayer Cropscience Nv | Optimisation ciblée du génome dans des plantes |
WO2019138083A1 (fr) | 2018-01-12 | 2019-07-18 | Basf Se | Gène sous-jacent au nombre de qtl d'épillets par épi de blé sur le chromosome 7a |
WO2019224359A1 (fr) | 2018-05-25 | 2019-11-28 | Basf Se | Plantes comprenant des gènes de restauration de la stérilité mâle cytoplasmique de type g du blé, et leurs utilisations |
WO2019224355A1 (fr) | 2018-05-25 | 2019-11-28 | Basf Se | Plantes comprenant des gènes de restauration de la stérilité mâle cytoplasmique de type g du blé, et leurs utilisations |
WO2019224353A1 (fr) | 2018-05-25 | 2019-11-28 | Basf Se | Plantes comprenant des gènes de restauration de la stérilité mâle cytoplasmique de type g du blé et leurs utilisations |
WO2019234231A1 (fr) | 2018-06-08 | 2019-12-12 | Basf Se | Plantes comprenant des gènes de restauration de la stérilité mâle cytoplasmique de type g du blé et leurs utilisations |
WO2023118541A1 (fr) | 2021-12-22 | 2023-06-29 | Basf Se | Molécules d'acide nucléique régulatrices permettant de modifier l'expression génique de plantes céréalières |
Also Published As
Publication number | Publication date |
---|---|
AU1162899A (en) | 1999-06-07 |
AR017630A1 (es) | 2001-09-12 |
CA2309880A1 (fr) | 1999-05-27 |
ZA9810498B (en) | 1999-05-24 |
ID21668A (id) | 1999-07-08 |
EP1032681A1 (fr) | 2000-09-06 |
MA24818A1 (fr) | 1999-12-31 |
FR2771104B1 (fr) | 2000-12-08 |
BR9815628A (pt) | 2000-10-24 |
FR2771104A1 (fr) | 1999-05-21 |
CO4950591A1 (es) | 2000-09-01 |
AU747634B2 (en) | 2002-05-16 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP0633317B1 (fr) | Séquence d'ADN isolée pouvant servir de zone terminatrice dans un gène chimère utilisable pour la transformation des plantes | |
CA2261094C (fr) | Gene chimere a plusieurs genes de tolerance herbicide, cellule vegetale et plante tolerantes a plusieurs herbicides | |
CA2219979C (fr) | Sequence adn d'un gene de l'hydroxy-phenyl pyruvate dioxygenase et obtention de plantes contenant un gene de l'hydroxy-phenyl pyruvate dioxygenase, tolerantes a certains herbicides | |
AU2016399292B2 (en) | Herbicide tolerant protein, encoding gene and use thereof | |
WO1999025842A1 (fr) | Gene chimere ayant un promoteur lumiere dependant conferant la tolerance aux inhibiteurs de l'hppd | |
CA2315677C (fr) | Promoteur h3c4 de mais associe au premier intron de l'actine de riz, gene chimere le comprenant et plante transformee | |
EP4032976A1 (fr) | Polypeptide mutant d'hydroxyphénylpyruvate dioxygénase, son gène codant et son utilisation | |
FR2961375A1 (fr) | Surproduction d'acide jasmonique dans des plantes transgeniques | |
EP3110832B1 (fr) | Plantes a rendement accru et methode d'obtention de telles plantes | |
WO2023206126A1 (fr) | Utilisation de la protoporphyrinogène oxydase | |
EP4339290A1 (fr) | Utilisation de la protoporphyrinogène oxydase | |
KR100480479B1 (ko) | 프로토포르피리노겐 옥시다아제의 엽록체 및 미토콘드리아동시발현을 이용한 작물의 제초제 저항성 증대 방법 및 이유전자를 제초제 저항성 선발 마커로 사용하는 형질전환세포주 선발 방법 | |
FR2796954A1 (fr) | Hydroxy-phenyl pyruvate dioxygenase fusionnee a un peptide signal, sequence d'adn et obtention de plantes contenant un tel gene, tolerantes aux herbicides | |
EP1315801A1 (fr) | Hydroxy-phenyl pyruvate dioxygenase fusionnee a un peptidesignal, sequence d 'adn et obtention de plantes contenant un tel gene,tolerantes aux herbicides | |
FR2796394A1 (fr) | Nouveau gene sgs3 de plante et son utilisation | |
WO2001055407A1 (fr) | Nouveau gene sgs2 de plante et son utilisation | |
FR2796395A1 (fr) | Nouveau gene sgs3 de plante et son utilisation |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A1 Designated state(s): AL AU BA BB BG BR BY CA CN CU CZ EE GE HR HU ID IL IS JP KP KR KZ LK LR LT LV MG MK MN MX NO NZ PL RO RU SG SI SK SL TR TT UA UZ VN YU |
|
AL | Designated countries for regional patents |
Kind code of ref document: A1 Designated state(s): GH GM KE LS MW SD SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG |
|
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
DFPE | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101) | ||
WWE | Wipo information: entry into national phase |
Ref document number: 1998954565 Country of ref document: EP |
|
ENP | Entry into the national phase |
Ref document number: 2309880 Country of ref document: CA Ref country code: CA Ref document number: 2309880 Kind code of ref document: A Format of ref document f/p: F |
|
NENP | Non-entry into the national phase |
Ref country code: KR |
|
WWE | Wipo information: entry into national phase |
Ref document number: 11628/99 Country of ref document: AU |
|
WWP | Wipo information: published in national office |
Ref document number: 1998954565 Country of ref document: EP |
|
WWG | Wipo information: grant in national office |
Ref document number: 11628/99 Country of ref document: AU |
|
WWW | Wipo information: withdrawn in national office |
Ref document number: 1998954565 Country of ref document: EP |