EP1026988A4 - Novel fluorescent reporter molecules and their applications including assays for caspases - Google Patents

Novel fluorescent reporter molecules and their applications including assays for caspases

Info

Publication number
EP1026988A4
EP1026988A4 EP98953317A EP98953317A EP1026988A4 EP 1026988 A4 EP1026988 A4 EP 1026988A4 EP 98953317 A EP98953317 A EP 98953317A EP 98953317 A EP98953317 A EP 98953317A EP 1026988 A4 EP1026988 A4 EP 1026988A4
Authority
EP
European Patent Office
Prior art keywords
caspases
applications including
reporter molecules
fluorescent reporter
novel fluorescent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP98953317A
Other languages
German (de)
French (fr)
Other versions
EP1026988A1 (en
Inventor
Eckard Weber
Sui Xiong Cai
John F W Keana
John A Drewe
Han-Zhong Zhang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytovia Therapeutics LLC
Original Assignee
Cytovia Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cytovia Inc filed Critical Cytovia Inc
Publication of EP1026988A1 publication Critical patent/EP1026988A1/en
Publication of EP1026988A4 publication Critical patent/EP1026988A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/06—Dipeptides
    • C07K5/06008—Dipeptides with the first amino acid being neutral
    • C07K5/06017—Dipeptides with the first amino acid being neutral and aliphatic
    • C07K5/06026—Dipeptides with the first amino acid being neutral and aliphatic the side chain containing 0 or 1 carbon atom, i.e. Gly or Ala
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K9/00—Peptides having up to 20 amino acids, containing saccharide radicals and having a fully defined sequence; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/06—Dipeptides
    • C07K5/06008—Dipeptides with the first amino acid being neutral
    • C07K5/06017—Dipeptides with the first amino acid being neutral and aliphatic
    • C07K5/06034—Dipeptides with the first amino acid being neutral and aliphatic the side chain containing 2 to 4 carbon atoms
    • C07K5/06052—Val-amino acid
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/08—Tripeptides
    • C07K5/0802—Tripeptides with the first amino acid being neutral
    • C07K5/0804—Tripeptides with the first amino acid being neutral and aliphatic
    • C07K5/0806—Tripeptides with the first amino acid being neutral and aliphatic the side chain containing 0 or 1 carbon atoms, i.e. Gly, Ala
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/08—Tripeptides
    • C07K5/0802—Tripeptides with the first amino acid being neutral
    • C07K5/0804—Tripeptides with the first amino acid being neutral and aliphatic
    • C07K5/0808—Tripeptides with the first amino acid being neutral and aliphatic the side chain containing 2 to 4 carbon atoms, e.g. Val, Ile, Leu
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/10—Tetrapeptides
    • C07K5/1002—Tetrapeptides with the first amino acid being neutral
    • C07K5/1005—Tetrapeptides with the first amino acid being neutral and aliphatic
    • C07K5/101—Tetrapeptides with the first amino acid being neutral and aliphatic the side chain containing 2 to 4 carbon atoms, e.g. Val, Ile, Leu
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/10—Tetrapeptides
    • C07K5/1002—Tetrapeptides with the first amino acid being neutral
    • C07K5/1005—Tetrapeptides with the first amino acid being neutral and aliphatic
    • C07K5/1013—Tetrapeptides with the first amino acid being neutral and aliphatic the side chain containing O or S as heteroatoms, e.g. Cys, Ser
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/10—Tetrapeptides
    • C07K5/1002—Tetrapeptides with the first amino acid being neutral
    • C07K5/1016—Tetrapeptides with the first amino acid being neutral and aromatic or cycloaliphatic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/10—Tetrapeptides
    • C07K5/1021—Tetrapeptides with the first amino acid being acidic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1488—Methods for deciding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Hematology (AREA)
  • Pathology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Dispersion Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
EP98953317A 1997-10-10 1998-10-09 Novel fluorescent reporter molecules and their applications including assays for caspases Withdrawn EP1026988A4 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US33661 1979-04-26
US6158297P 1997-10-10 1997-10-10
US61582P 1997-10-10
US3366198A 1998-03-03 1998-03-03
PCT/US1998/021231 WO1999018856A1 (en) 1997-10-10 1998-10-09 Novel fluorescent reporter molecules and their applications including assays for caspases

Publications (2)

Publication Number Publication Date
EP1026988A1 EP1026988A1 (en) 2000-08-16
EP1026988A4 true EP1026988A4 (en) 2005-03-30

Family

ID=26709974

Family Applications (1)

Application Number Title Priority Date Filing Date
EP98953317A Withdrawn EP1026988A4 (en) 1997-10-10 1998-10-09 Novel fluorescent reporter molecules and their applications including assays for caspases

Country Status (15)

Country Link
EP (1) EP1026988A4 (en)
JP (1) JP2001519368A (en)
KR (1) KR20010031056A (en)
CN (1) CN1281346A (en)
AU (1) AU754634B2 (en)
BR (1) BR9814816A (en)
CA (1) CA2308125A1 (en)
EA (1) EA200000408A1 (en)
HU (1) HUP0100079A2 (en)
IL (1) IL135365A0 (en)
IS (1) IS5414A (en)
NO (1) NO20001322L (en)
NZ (1) NZ503619A (en)
PL (1) PL341661A1 (en)
WO (1) WO1999018856A1 (en)

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US6342611B1 (en) 1997-10-10 2002-01-29 Cytovia, Inc. Fluorogenic or fluorescent reporter molecules and their applications for whole-cell fluorescence screening assays for capsases and other enzymes and the use thereof
ATE276754T1 (en) 1998-07-21 2004-10-15 Cytovia Inc NEW FLUORESCENCE DYES AND THEIR APPLICATION IN WHOLE CELL FLUORESCENCE DETECTION PROCEDURES FOR CASPASES, PEPTIDASES, PROTEASES AND OTHER ENZYMES AND THEIR USE
ATE250140T1 (en) * 1999-03-12 2003-10-15 Evotec Technologies Gmbh CHEMOSENSITIVITY MEASUREMENT VIA CASPASE ACTIVITY
EP1055929A1 (en) * 1999-05-26 2000-11-29 Tibotec N.V. Means and methods for drug discovery and the phenotypic characterisation of cells
WO2000073437A1 (en) * 1999-05-27 2000-12-07 Merck Frosst Canada & Co. Assays for caspase activity using green fluorescent proteins
US6261794B1 (en) 1999-10-14 2001-07-17 Saint Louis University Methods for identifying inhibitors of methionine aminopeptidases
PT1230232E (en) 1999-11-05 2004-07-30 Cytovia Inc 4H-CROMENE AND ITS ANALOGS AS ACTIVATORS OF APOPTOSE CASPASES AND INDUCTORS AND THEIR UTILIZATION
GB9927331D0 (en) 1999-11-18 2000-01-12 Fluorescience Ltd Assay for measuring enzyme activity in vivo
US6861419B2 (en) 2000-04-18 2005-03-01 Cytovia, Inc. Substituted 1, 4-thiazepine and analogs as activators of caspases and inducers of apoptosis and the use thereof
US6828091B2 (en) 2000-08-03 2004-12-07 Cytovia, Inc. Method of identifying immunosuppressive agents
DE10053747C2 (en) * 2000-10-30 2002-10-24 Deutsches Krebsforsch Method for the simultaneous determination of two fluorescence emissions with a single laser flow cytometer
EP1205540A1 (en) 2000-11-10 2002-05-15 Evotec OAI AG Method of measuring cell vitality
US20020128292A1 (en) 2000-12-07 2002-09-12 Cai Sui Xiong Substituted indole-2-carboxylic acid benzylidene-hydrazides and analogs as activators of caspases and inducers of apoptosis and the use thereof
US6716851B2 (en) 2000-12-12 2004-04-06 Cytovia, Inc. Substituted 2-aryl-4-arylaminopyrimidines and analogs as activators or caspases and inducers of apoptosis and the use thereof
WO2002072544A2 (en) 2001-03-14 2002-09-19 Cytovia, Inc. Multifluoro-substituted chalcones and analogs as activators of caspases and inducers of apoptosis and the use thereof
US6979530B2 (en) 2001-05-21 2005-12-27 Applera Corporation Peptide conjugates and fluorescence detection methods for intracellular caspase assay
US7148030B2 (en) 2002-02-01 2006-12-12 Promega Corporation Bioluminescent protease assay
CN100349887C (en) 2002-05-16 2007-11-21 西托维亚公司 Substituted 4-aryl-4H-pyrrolo[2,3-H]chromenes and analogs as activators of caspases and inducers of apoptosis and the use thereof
AU2003241482A1 (en) 2002-05-16 2003-12-02 Cytovia, Inc. Substituted 4h-chromenes, 2h-chromenes, chromans and analogs as activators of caspases and inducers of apoptosis and the use thereof
DE60235652D1 (en) * 2002-07-08 2010-04-22 Ge Healthcare Uk Ltd REAGENTS AND A PROCESS FOR SATURATING MARKING OF PROTEINS
US8252520B2 (en) * 2002-10-11 2012-08-28 Taivex Therapeutics Corporation Methods and compounds for inhibiting Hec1 activity for the treatment of proliferative diseases
US6821744B2 (en) 2002-10-29 2004-11-23 Roche Diagnostics Operations, Inc. Method, assay, and kit for quantifying HIV protease inhibitors
EP1660092A2 (en) 2003-07-03 2006-05-31 Myriad Genetics, Inc. 4-arylamino-quinazolines as activators of caspases and inducers of apoptosis
WO2005047245A2 (en) 2003-11-14 2005-05-26 Wisconsin Alumni Research Foundation Fluorescence assays with improved blocking groups
WO2005071095A2 (en) 2004-01-16 2005-08-04 Applera Corporation Fluorogenic kinase assays and substrates for kinases and phosphatases
US7553632B2 (en) 2004-01-22 2009-06-30 Promega Corporation Luminogenic and nonluminogenic multiplex assay
US7416854B2 (en) * 2004-01-22 2008-08-26 Promega Corporation Luminogenic and nonluminogenic multiplex assay
SI1942898T2 (en) 2005-09-14 2014-08-29 Takeda Pharmaceutical Company Limited Dipeptidyl peptidase inhibitors for treating diabetes
CN102675221A (en) 2005-09-16 2012-09-19 武田药品工业株式会社 Intermediate in method for preparing pyrimidinedione derivative
EP1968641B1 (en) * 2005-12-12 2014-06-11 Innova Biosciences Limited Production of conjugates
WO2007112347A1 (en) 2006-03-28 2007-10-04 Takeda Pharmaceutical Company Limited Dipeptidyl peptidase inhibitors
TW200838536A (en) 2006-11-29 2008-10-01 Takeda Pharmaceutical Polymorphs of succinate salt of 2-[6-(3-amino-piperidin-1-yl)-3-methyl-2,4-dioxo-3,4-dihydro-2H-pyrimidin-1-ylmethy]-4-fluor-benzonitrile and methods of use therefor
EP2142665B1 (en) 2007-04-13 2013-11-27 Promega Corporation Luminescent live and dead cell assay
KR100909996B1 (en) * 2007-09-19 2009-07-29 한국생명공학연구원 Fusion protein for detecting the activity of caspase-3 and a method for detecting the activity of caspase-3 using the same
CN102603695B (en) * 2012-02-10 2014-06-18 山东大学 Amino acid-fluorophore compound and application thereof
ES2655212T3 (en) 2012-07-19 2018-02-19 Kingston University Higher Education Corporation Fluorescent probe based on rhodamine to determine coagulase activity and detection of bacterial strains producing coagulase
CN104458709A (en) * 2013-09-12 2015-03-25 中国药科大学 High flux screening method for screening calcium activated neutral protease-1 inhibitor
US10383956B2 (en) 2014-07-11 2019-08-20 The University Of Tokyo Fluorescent probe for detecting dipeptidyl peptidase IV
CN104263356B (en) * 2014-09-29 2016-02-24 辽宁大学 A kind of rhodamine pH fluorescent probe and application thereof containing methionine(Met) structure
CN106146611B (en) * 2015-05-14 2019-09-17 中国科学院大连化学物理研究所 It is a kind of measure dipeptidyl peptidase IV activity fluorescence probe substrate and its application
WO2017223147A1 (en) 2016-06-23 2017-12-28 Life Technologies Corporation Methods and compositions for detecting or measuring caspases or apoptosis
WO2018071556A1 (en) 2016-10-11 2018-04-19 The Regents Of The University Of California Detection, identification, and purification of degradative and non-degradative enzymes in biological samples
CN107987019A (en) * 2018-01-08 2018-05-04 深圳市佶达德科技有限公司 A kind of preparation method of rhodamine double pyrazole acetic acid esters organic laser material
CN108129388A (en) * 2018-01-08 2018-06-08 深圳市佶达德科技有限公司 A kind of rhodamine double pyrazole acetic acid esters organic laser material and its application
CN108760234B (en) * 2018-06-05 2020-05-15 哈尔滨工程大学 Method and device for synchronously testing fluid flow and solid motion information based on PIV (particle image velocimetry) and PTV (particle beam velocimetry) technologies
KR102138256B1 (en) * 2018-09-13 2020-08-13 한국과학기술연구원 probe for measuring the activity of caspase-1 and composition for diagnosis of inflammatory diseases comprising the same
CN112961209B (en) * 2021-04-07 2022-06-24 中国科学院武汉病毒研究所 caspase inhibitors and uses thereof
CN114107435A (en) * 2021-11-30 2022-03-01 广东省人民医院 Activatable photoacoustic-fluorescence dual-mode probe for real-time monitoring of immunotherapy and application
CN117143949B (en) * 2023-08-31 2024-04-05 青岛海洋食品营养与健康创新研究院 Euphausia superba source high F value oligopeptide and application thereof in liver protection

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Also Published As

Publication number Publication date
EP1026988A1 (en) 2000-08-16
PL341661A1 (en) 2001-04-23
JP2001519368A (en) 2001-10-23
IS5414A (en) 2000-03-24
CN1281346A (en) 2001-01-24
BR9814816A (en) 2004-06-22
WO1999018856A1 (en) 1999-04-22
HUP0100079A2 (en) 2001-05-28
EA200000408A1 (en) 2000-12-25
NO20001322L (en) 2000-06-13
AU1072299A (en) 1999-05-03
KR20010031056A (en) 2001-04-16
CA2308125A1 (en) 1999-04-22
NZ503619A (en) 2001-11-30
NO20001322D0 (en) 2000-03-14
AU754634B2 (en) 2002-11-21
IL135365A0 (en) 2001-05-20

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