EP1021557A1 - Dispositif de surveillance microbienne - Google Patents

Dispositif de surveillance microbienne

Info

Publication number
EP1021557A1
EP1021557A1 EP97943349A EP97943349A EP1021557A1 EP 1021557 A1 EP1021557 A1 EP 1021557A1 EP 97943349 A EP97943349 A EP 97943349A EP 97943349 A EP97943349 A EP 97943349A EP 1021557 A1 EP1021557 A1 EP 1021557A1
Authority
EP
European Patent Office
Prior art keywords
fluorescent
fluorescent compound
compound
matrix
respiring
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP97943349A
Other languages
German (de)
English (en)
Inventor
David T. Stitt
Gregory J. Burrell
Shawn Beaty
Joanna Kwok Yu Hu
James F. Monthony
Robert Sapitowicz
Timothy G. Foley
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
Original Assignee
Becton Dickinson and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co
Publication of EP1021557A1 publication Critical patent/EP1021557A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/18—Testing for antimicrobial activity of a material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2304/00—Chemical means of detecting microorganisms
    • C12Q2304/40—Detection of gases
    • C12Q2304/44—Oxygen

Definitions

  • the processes of the instant invention use a fluorescence detection system wherein the fluorescing sensor compound is one which exhibits a quantifiable degree of quenching when exposed to oxygen.
  • the sensor compound may be brought into contact with the test sample (either directly or separated by an oxygen permeable membrane) and the fluorescence is measured or observed visually with appropriate aids.
  • an increase in fluorescence is indicative of respiring aerobic microorganisms, which utilize (and thereby reduce) t thhee o oxxvygeeenn i inn t thhee s saammopllee
  • Figure 2 graphically depicts the intensity of fluorescence as a function of time for indicators in contact with broth inoculated with different concentrations of microorganisms.
  • Figure 4 graphically depicts the intensity of fluorescence as a function of time for indicators in contact with broth inoculated with the same number of organisms but containing different amounts of copper sulfate.
  • the system can be allowed to interact unobserved for a predetermined amount of time after which the presence or absence of fluorescence is observed and compared to appropnate control samples, yielding results that are often obtained with a smgle such observation
  • a particular benefit of this system is that the measurement of fluorescence is non-destructive and if after a penod of time (e g 4 hours) the results are non-conclusive, the system can be re-incubated and read aga at a later tune
  • reagent controls such is by no means necessary, and it is postulated that, by appropnate choice of fluorescent compounds, a skilled technician or technologist would be capable of mdependently determining whether the results mdicate the , presence of microbial activity
  • the fluorophore can also be in a liquid phase separated from the solution being analyzed by a membrane that is impermeable to the indicator molecules and to microorganisms in the sample but which is permeable to oxygen. Additionally, less- sensitive sensors can be fabricated by using less 0-2 permeable polymers or by using compounds with shorter excited-state lifetimes.
  • the wells containing the highest concentrations of reducing agent have the highest fluorescence intensity, thus demonstrating the relationship between O2 concentration and fluorescence.
  • EXAMPLE 10 Effect of Antibiotics on the Oxygen Consumption of Microorganisms Using DPA Fluorescence Indicator
  • a 1 x 10 7 CFU/mL suspension of E. coli ATCC #25922 in Mueller Hinton broth was prepared; 150 microliter aliquots were pipetted into the odd numbered rows of a microtiter tray, while 150 microliter aliquots of uninoculated Mueller Hinton broth were pipetted into the wells of the even numbered columns.
  • the lids containing the indicator coated prongs were placed on the trays.
  • the lidded trays were placed in a 37°C high humidity incubator for 3 hours.

Abstract

La présente invention concerne un procédé de détection de la présence de micro-organismes respirants dans un fluide. Ledit procédé se déroule de la façon suivante: (i) placer le fluide dans un récipient dans lequel il sera sensiblement isolé de l'oxygène de l'air. Un détecteur sera ensuite placé à l'intérieur dudit récipient, sans qu'il soit en contact direct avec le fluide, la composition de ce détecteur consistant en un composé fluorescent présentant une intensité fluorescente réduite lorsqu'il est soumis à un rayonnement à des longueurs d'ondes de lumière; (ii) soumettre à un rayonnement cette composition de détecteur avec des longueurs d'ondes de lumière de façon à ce que ledit composé fluorescent émette une fluorescence; (iii) mesurer ou observer visuellement l'intensité lumineuse fluorescente provenant de ce composé fluorescent tout en soumettant le composé du détecteur à un rayonnement avec ladite lumière; (iv) comparer cette mesure avec celle effectuée sur un témoin exempt de micro-organismes respirants, ce témoin étant tiré du groupe composé d'un témoin réactif n'étant pas en contact avec des micro-organismes respirants et d'un seuil calculé, dans lequel le changement de l'intensité fluorescente par rapport à l'intensité fluorescente du témoin indique la présence de micro-organismes respirants; et (v) au cas où aucune augmentation n'a été mesurée ou observée, répéter, selon les besoins, les étapes (ii), (iii) et (iv), afin de détecter la présence de micro-organismes respirants dans ledit fluide.
EP97943349A 1996-09-18 1997-09-18 Dispositif de surveillance microbienne Withdrawn EP1021557A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US71555796A 1996-09-18 1996-09-18
US715557 1996-09-18
PCT/US1997/016496 WO1998012348A1 (fr) 1996-09-18 1997-09-18 Dispositif de surveillance microbienne

Publications (1)

Publication Number Publication Date
EP1021557A1 true EP1021557A1 (fr) 2000-07-26

Family

ID=24874543

Family Applications (1)

Application Number Title Priority Date Filing Date
EP97943349A Withdrawn EP1021557A1 (fr) 1996-09-18 1997-09-18 Dispositif de surveillance microbienne

Country Status (5)

Country Link
EP (1) EP1021557A1 (fr)
JP (1) JP2002501363A (fr)
AU (1) AU4483997A (fr)
CA (1) CA2264272A1 (fr)
WO (1) WO1998012348A1 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6395506B1 (en) * 1991-04-18 2002-05-28 Becton, Dickinson And Company Device for monitoring cells
US5998517A (en) * 1998-06-05 1999-12-07 Becton, Dickinson And Company Composition for the detection of microorganisms in a sample
US6432697B1 (en) * 2000-02-03 2002-08-13 Becton, Dickinson And Company Transparent sample container
EP1134583A1 (fr) * 2000-03-17 2001-09-19 Nederlandse Organisatie voor toegepast-natuurwetenschappelijk Onderzoek TNO Mesure de changements du métabolisme
US20050059140A1 (en) * 2003-09-12 2005-03-17 Andrea Liebmann-Vinson Methods of surface modification to enhance cell adhesion
EP2245177B1 (fr) * 2008-02-19 2015-05-13 Becton Dickinson and Company Systèmes et procédés pour identifier une culture comme étant positive pour des micro-organismes avec un degré de confiance élevé
CN101978068B (zh) * 2008-02-19 2016-04-20 贝克顿·迪金森公司 用于推测性鉴定培养物内微生物类型的系统和方法
US8613158B2 (en) 2008-04-18 2013-12-24 Ball Horticultural Company Method for grouping a plurality of growth-induced seeds for commercial use or sale based on testing of each individual seed
JP5901689B2 (ja) * 2014-05-07 2016-04-13 ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ 培養物中の微生物の種類の仮同定のためのシステムおよび方法
RU2576030C1 (ru) * 2015-03-04 2016-02-27 Сергей Дмитриевич Иванов Способ определения опасности микробиологической загрязненности воды

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1261717A (fr) * 1982-12-23 1989-09-26 John R. Bacon Methode et appareil pour mesurer l'oxygene
US4945060A (en) * 1988-03-15 1990-07-31 Akzo N. V. Device for detecting microorganisms
ATE132537T1 (de) * 1990-03-29 1996-01-15 Avl Photronics Corp Verfahren und apparat zum nachweis biologischer aktivitäten in einer probe
AU647609B2 (en) * 1991-04-18 1994-03-24 Becton Dickinson & Company Microbial monitoring device

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO9812348A1 *

Also Published As

Publication number Publication date
JP2002501363A (ja) 2002-01-15
CA2264272A1 (fr) 1998-03-26
AU4483997A (en) 1998-04-14
WO1998012348A1 (fr) 1998-03-26

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