EP0931150A2 - Modulation genetique du murissement de fruits - Google Patents

Modulation genetique du murissement de fruits

Info

Publication number
EP0931150A2
EP0931150A2 EP97939069A EP97939069A EP0931150A2 EP 0931150 A2 EP0931150 A2 EP 0931150A2 EP 97939069 A EP97939069 A EP 97939069A EP 97939069 A EP97939069 A EP 97939069A EP 0931150 A2 EP0931150 A2 EP 0931150A2
Authority
EP
European Patent Office
Prior art keywords
seq
plants
sequence
ripening
plant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP97939069A
Other languages
German (de)
English (en)
Inventor
Graham Barron Seymour
Colin Roger Bird
Rosybel de Jesús MEDINA-SUAREZ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Syngenta Ltd
Original Assignee
Zeneca Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9618862.8A external-priority patent/GB9618862D0/en
Priority claimed from GBGB9708366.1A external-priority patent/GB9708366D0/en
Application filed by Zeneca Ltd filed Critical Zeneca Ltd
Publication of EP0931150A2 publication Critical patent/EP0931150A2/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8249—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving ethylene biosynthesis, senescence or fruit development, e.g. modified tomato ripening, cut flower shelf-life

Definitions

  • This invention relates generally to the modification of a plant phenotype by the regulation of plant gene expression. More specifically it relates to the modulation of the ripening and/or tissue senescence characteristics and plants derived therefrom.
  • One suitable application of the present invention is the modulation of ripening and/or senescence processes in plants of the genus Musa (referred to herein as banana).
  • Overexpression may be achieved by insertion of one or more than one extra copies of the selected gene. It is, however, not unknown for plants or their progeny. originally transformed with one or more than one extra copy of a nucleotide sequence, to exhibit the effects of underexpression as well as overexpression.
  • Gene control by any of these methods requires the insertion of a selected gene or genes into plant material which can be regenerated into plants.
  • This transformation process can be performed via a number of methods, for example: the Agrobacterium-m ⁇ diatsd transformation method.
  • microparticles of dense material are fired at high velocity at the target cells where they penetrate the cells, opening an aperture in the cell wall through which DNA may enter.
  • the DNA may be coated on to the microparticles or may be added to the culture medium.
  • microinjection. the DNA is inserted by injection into individual cells via an ultrafine hollow needle.
  • Another method viz. fibre-mediated transformation, applicable to both monocots and dicots, involves creating a suspension of the target cells in a liquid, adding microscopic needle-like material, such as silicon carbide or silicon nitride "whiskers", .and agitating so that the cells and whiskers collide and DNA present in the liquid enters the cell.
  • Bananas are a globally important fruit crop. They are not only a popular dessert fruit, but represent a vital carbohydrate staple in the tropics with as many as 100 million people subsisting on bananas and plantains as their main energy source.
  • the cultivated dessert banana is commonly triploid, parthenocarpic and belongs to the Musa AAA genome group, eg. Cavendish subtypes.
  • Bananas are climacteric fruits and ripening is regulated by ethylene produced by the fruit and involves numerous biochemical changes including the conversion of starch to sugars, cell wall disassembly, synthesis of volatile compounds, changes in phenolic constituents and degradation of chlorophyll in the peel.
  • a method of modulating the ripening or tissue senescence process in pl.ants of the genus Musa comprising inserting into plant material at least one polynucleotide sequence selected from the sequences depicted as SEQ ID-Nos. 1 -57. regenerating said plant material and selecting from the transformed regenerants, plants with modulated ripening or tissue senescence characteristics.
  • the said polynucleotide may be obtained from the cDNA library having the NCIMB Accession Number 40814.
  • a method of modulating the ripening or tissue senescence process in plants of the genus Musa comprising inserting into plant material at least one polynucleotide sequence or a fragment thereof, obtainable by hybridisation, from the cDNA library having the NCIMB Accession Number 40814, by the use of at least one of the sequences depicted as SEQ ID Nos 1-57 as oligonucleotide probes, said hybridisation being conducted at a temperature from 60°C to 65°C in 0.3 strength citrate buffered saline containing 0.1% SDS followed by rinsing at the same temperature with 0.3 strength citrate buffered saline containing 0.1% SDS, regenerating said plant material and selecting from the transformed regenerants, plants with modulated ripening or tissue senescence characteristics.
  • the invention further provides a method as described above wherein the said polynucleotide is capable of modulating the production of pectate lyase and more specifically the polynucleotide comprises at least one of the sequences depicted in the sequence listings as SEQ-ID-Nos. 13-18.
  • a preferred method for inserting the said polynucleotides into plant material according to the method of the present invention may be selected from the group comprising the Agrobacte ⁇ um, microparticle bombardment, fibre mediated or direct insertion methods.
  • the invention further provides plants, their progeny and seed and material obtained from said plants, produced according to the method of the present invention. It is preferred that the said plants, their progeny and seed and material obtained from said plants are derived from plants of the genus Musa.
  • the present invention also provides a vector functional in plants comprising a promoter region which is operable in plant cells, at least one of the polynucleotide sequences described above and a transcription termination sequence.
  • the promoter may be constitutive, developmentally regulated or switchable.
  • the promoter may also be tissue specific or organ specific.
  • a banana produced via the preceding method having altered fruit characteristics when compared with a banana which is not transformed with at least one of the polynucleotide sequences described above.
  • the present invention also provides an improved method of controlling plant pathogens comp ⁇ sing the application of an anti-pathogenic agent to plants, characterised in that plants to which the said agent is applied, are plants according to the present invention.
  • the gene sequences of the present invention may be synthesised ab initio, using the sequence data in the sequence listing provided herewith, or isolated from a library using the standard techniques know within the art.
  • sequences depicted in the sequence listing or parts thereof may also be used to create oligonucleotide probes for the purposes of isolating from the library those polynucleotides which are capable of producing the desired proteins.
  • oligonucleotide probes for the purposes of isolating from the library those polynucleotides which are capable of producing the desired proteins.
  • To assist the isolation of these polynucleotides we have deposited with the National Collection of Industrial & Marine Bacte ⁇ a, St. Machar Drive, Aberdeen, UK, a cDNA library of the banana peel ripening related genes. The library was deposited on 9th July 1996 and has the NCIMB Accession Number 40814.
  • this invention is based on the identification of genes which encode proteins involved in banana ripening-related processes, specifically within banana pulp.
  • DNA sequences according to the sequence listing or those sequences obtainable from the deposited library may be used in the process of modifying the plant ripening characte ⁇ stics of plants and/or fruit.
  • banana plants can be generated which, amongst other phenotypic modifications, may have one or more of the following fruit characte ⁇ stics: improved resistance to damage du ⁇ ng harvest, packaging and transportation due to slowing of the ⁇ pening and over- ⁇ penmg processes; longer shelf life and better storage characte ⁇ stics due to reduced activity of degradative pathways (e.g.
  • improved processing characte ⁇ stics due to changed activity of proteins/enzymes contributing to factors such as: viscosity, solids, pH, elasticity; improved flavour and aroma at the point of sale due to modification of the sugar/acid balance and other flavour and aroma components responsible for characteristics of the ⁇ pe fruit; modified colour due to changes m activity of enzymes involved in the pathways of pigment biosynthesis (e.g. lycopene, ⁇ -carotene. chalcones and anthocyamns), increased resistance to post-harvest pathogens such as fungi.
  • the activity of the ⁇ pemng-related proteins may be either increased or reduced depending on the characte ⁇ stics desired for the modified plant part (fruit, leaf, flower, etc).
  • the levels of protein may be increased: for example, by incorporation of additional genes.
  • the additional genes may be designed to give either the same or different spatial and temporal patterns of expression m the fruit "Antisense” or "partial sense” or other techniques may be used to reduce the expression of ripening-related protein.
  • the activity of each ripening-related protein or enzyme may be modified either individually or in combination with modification of the activity of one or more other ripening- related proteins/enzymes.
  • the activities of the ripening-related proteins/enzymes may be modified in combination with modification of the activity of other enzymes involved in fruit ripening or related processes.
  • DNA constructs according to the invention for gene silencing may comp ⁇ se a base sequence at least 10 bases (preferably at least 35 bases) in length for transc ⁇ ption into RNA. There is no theoretical upper limit to the base sequence, it may be as long as the relevant mRNA produced by the cell but for convenience it will generally be found suitable to use sequences between 100 and 1000 bases in length. When using genomic DNA as the source of a base sequence for transc ⁇ ption it is possible to use either intron or exon regions or a combination of both.
  • a suitable cDNA or genomic DNA or synthetic polynucleotide may be used as a source of the DNA base sequence for transcription.
  • the isolation of suitable npening-related sequences is desc ⁇ bed above; it is convenient to use DNA sequences derived from the ripening-related clones deposited at NCIMB in Aberdeen. Sequences coding for the whole. or substantially the whole, of the approp ⁇ ate npening-related protein may thus be obtained Suitable lengths of this DNA sequence may be cut out for use by means of rest ⁇ ction enzymes.
  • Constructs suitable for expression of the approp ⁇ ate ripening-related sequence in banana cells may be produced using a cDNA sequence selected from the deposited library having the NCIMB Accession Number 40814 or the gene sequence as found in the chromosome of the banana plant. Recombinant DNA constructs may be made using standard techniques.
  • modulation means an increase or decrease of the desired effect More specifically ''modulation of the npening or tissue senescence process in plants of the genus Musa " means an increase or decrease in production of a ⁇ pemng related protein resulting from the method as desc ⁇ bed above For example, where an increased ⁇ pemng related protein is desired, plants may be transformed according to the method as desc ⁇ bed above and those plants exhibiting the desired effect may be selected from the population of trans formants. Furthermore, it may be desirable to provide a plant with modulated ripening or tissue senescence characteristics by increasing the production of one protein and decreasing the production of another protein in the same plant.
  • a banana fruit, modified using the present method, and having decreased levels of the enzyme pectate lyase would be beneficial because pulp softening would require a substantially longer time when compared with a control.
  • another ripening related protein such as an antifungal protein in the same fruit using the present method, would complement the extended life of the banana pulp with increased resistance to disease.
  • Increase in production of a ripening related protein includes inserting into plant material one or more copies of any of the polynucleotides described above, wherein the said polynucleotides must be capable of producing a protein and thereby increasing protein levels when compared with a control plant.
  • Decrease in production of a ripening related protein includes inserting into plant material one or more copies of any of the polynucleotides described above, wherein the said polynucleotides must be capable of producing an mR A which is capable of interfering with endogenous mRNA to such a degree that the levels of translated protein are reduced when compared with a control plant.
  • Ripening process of plants means the process of maturing or developing .
  • Senescence means the progressive deterioration in function of cells, tissues, organs etc.. related to the period of time since that function commenced..
  • Control plant means a comparable plant used for the purposes of determining modulation of the ⁇ pening or tissue senescence process effect in plants.
  • the control pl ⁇ int is usually of the same species and variety as the material used before the transformation process and is grown in the same conditions, (usually with the transformant selection step modified in some way on the part of the control plant), as the transformed plants.
  • the control plant may comprise an untransformed control plant or a transformed control plant providing it has not already been transformed with the same polynucleotide sequence as the plant material to be transformed.
  • Plant material includes plant ceils and any other type of plant regenerable material. The following examples further illustrate the invention but are not to be construed to limit the scope thereof:
  • FIGURE 1 Plant transformation vector pUN. containing the UBI polyubiquitin promoter.
  • FIGURE 2 Plant transformation vector pSHYN, containing hygromycin resistance gene for selection of transformed plants.
  • FIGURE 3 Plant transformation vector pAN. containing the banana ACC oxidase promoter.
  • the first and second strands of the cDNAs were svnthesised from the messenger RNAs using a commercial cDNA synthesis kit (Catalog No. 200450, ZAP ExpressTM Gold Cloning kit. Stratagene Ltd. Cambridge. Ca bs, UK). Double stranded cDNAs were cloned into the ZAP ExpressTM vector, packaged, mixed with plating bacteria to determine titre and for library screening, following instructions of the suppliers protocol. 1.3 Screening of the cDNA library from banana pulp.
  • the unamplified cDNA library from ripening banana pulp was differentially screened using cDNA from unripe and ripening banana peel tissue.
  • a proportion of the library was plated individually at low density and duplicate plaque lifts made onto Hybond N nylon filters (Amersham) according to t e manufacturer's instructions.
  • One filter was hybridised to dCTP radiolabelled cDNA from green fruit and the duplicate filter hybridised to dCTP radiolabelled cDNA from ripening fruit. Hybridization's were at high stringency. Plaques hybridising preferentially with ripening or green radiolabelled cDNA were picked and re- plated for a second round of selection by differential screening. These clones were numbered as ripening up- or down-regulated peel clones. The clones were in-vivo excised from the ZAP expressTM vector into the pBK-CMV phagemid vector using the E.xAssistTM interference-resistant helper phage, following instructions from manufacturers protocol.
  • the ripening cDNA library from pulp tissue were prepared with an efficiency of 3.2 x 10 5 plaque-forming units per microgram of cDNA.
  • the sizes of the inserts in the peel library was 0.4 - 6.7 Kb with a mean size insert of 1.47 Kb.
  • a vector is constructed using the sequences corresponding to a fragment of the inserts of one of the sequences 1 to 57. This fragment is svnthesised by polymerase chain reaction using synthetic p ⁇ mers incorporating BamHI rest ⁇ ction sites suitable for clomng between a maize UBI polyubiquitin promoter (Christensen et al, 1992, Plant Molecular Biology,
  • the truncated sense expression cassette is excised by digestion with Ascl, the ends of the fragment are made flush with T4 polymerase and it is cloned into the vector pSHYN (Fig. 2.) which has been cut with Kpnl and the ends made flush with Klenow polymerase.
  • pSHYN contains hygromycin resistance gene for selection of transformed plants.
  • a vector is constructed using the sequences corresponding to a fragment of the inserts of one of the sequences 1 to 57. This fragment is synthesised by polymerase chain reaction using synthetic primers incorporating BamHI restriction sites suitable for cloning between a maize UBI polyubiquitin promoter (Christensen et al, 1992, Plant Molecular Biology, 18:675-689) and a nopaline synthase 3'end termination sequences in the vector pAN.
  • the truncated sense expression cassette is excised by digestion with Ascl, the ends of the fragment are made flush with T4 polymerase and it is cloned into the vector pSHYN (Fig. 2.) which has been cut with Kpnl and the ends made flush with Klenow polymerase.
  • pSHYN contains hygromycin resistance gene for selection of transformed plants.
  • Transformed Musa plants containing the vectors are produced by the method described in Sagi et al. (1995) Biotechnology. Vol. 13 pp 481-485. Regenerated transformed plants are identified by their ability to grow on hygromycin and grown to maturity. Ripening fruit are analysed for a modulation in their ripening related or senescence characteristics. Other suitable transformation methods for banana are described in Sagi et al. ( 1994) Plant Cell Reports. Vol. 13. pp 262-266. and May et al. (1995) Biotechnology. Vol. 13 pp 486- 492.
  • GCGAGAAGAG CAAAATCCTG ATCATCGGGG GCACCGGGTA CATCGGCAAG TTCATCGTGT 120
  • NTTTCCTCCC GGTATTANGA CAGGCAGGAG CCACTGGTCC TCCCACGGAC AAGGTTGTCA 600
  • TCTTANGTGA CGGGAACACA AAAGCGATCT TTCTCAATGA ANACACATCC GGACNTNCAC 660
  • TANGACAGGC AGGACCACTG GTCCTCCCCA CNGACAAGGT TGTCNTCTTA GGTGACNGGA 600
  • CAAGCCCTCA GGAACTCCAA CATCCAAGTC CTGTTGGATG TCCCCCGATC CGACGTGCAG 300
  • CCTGCAACAT CTTCTCCACC CAGGACCACG CCGCTGCCGC CATTGCCCGC GACTCCGCCG 360 CCGTCTTCGC CTGGAAGGGA GAGACCCTCG CCGAGTACTG GTGGTGCACC GAGCGATGCC 420 TCGACTGGGG CCCCAACGGT GGCCCCGACC TCATCGTCGA CAAAGGGGGT GACGCCACTC 480
  • CTCCAGGTCN AACCCCNAAA ATTACCGCCC AGATNAAAGA ACCCTCCTCC GCTTTTTGAA 660
  • GAAAACANCN CTGGCGTTCA NCGAATCTAC CANATGCCAG GGCCANCNGG GGCCCCTTGC 720
  • CTCCGGACTT CGGCAACTTT TTCAAGGACC ATGGCGGCTC CTGGGANACA CCCTACGGGC 540
  • ANCATGATTC ATCCCGGGCN TGGACTTCTC NGAAGGGGAA CANTCCTCAG GGTGTTCCGG 840
  • NTTANATTNC ACCGTCCTCC AATTNTCCCC ANTTTCTTTN GCCGCNGGNN NCGGGCCCCN 720
  • TGGTCATCCA GCTGAAGGAA GGAGCTCATC ATGAAACTCC CACANAGACC ATCGACGGCC 480 GGGGCGCCAG CGTCCACATC TCCGGGGGGC CGTGCATCAC CACCAGTNCG TCNCCAACAT 540
  • GCCTCCACAT CCACGACTGC AAGCCCACCG
  • TCTCCTTCTC 120 ACTATGGATG GAGAACCATG GCTGATGGGG ATGCCGTTTC CATTTTCGGC TCCAGCCACA 180
  • AATCNCC 727 (2) INFORMATION FOR SEQ ID NO: 17:
  • CCGGCAGCGG CTCGCTGACT GCGCCATCGG GTTCGGGAAG AACGCGATTT GGGGCAGGGA 300 CGGCGAGATA TACGTGGTGA CCGACAGTGG CGACNACGAC CCCGTCAATC CGAAAACGGG 360
  • CTTCCNTCCC AACCCCCATT TCCTTGGGAT TNCCCCCCGC CAATTCCCCC ATTTTCCAAC 780
  • NAACTTNCTT TGAACCCNCC CNTGAANAAG GTCCTTGCTG CTGGGTCCCA CCAACAATTT 840
  • NA 842
  • TGGGGAGCCT CACGTGGTGG TACAACGAGG AGGAGGACTC CGACTTCTCC ATCGCCTTCT 360 TAGGCGATAC CGCGACAGCT GTGCGACCGG GCGACATCGC CTACTTCTTC TTGGCAGGAT 420
  • TGANCGCGTA NCGGCATATA TCAATGTGAA AAGTGGTGGC TGTTCTGCCT CGGTGACCCT 660 TNATNAACTG GCNGCNCTGG GAAGATCAGG TTCTCCGTCN ACCTCCAAAA ATCCANCCTA 720
  • TCCACTCTCN CTGGGANAAT TTTGAAAAAT GTTNAGCTTC TAAATGATCT CCNGGACCNA 720
  • TGGTGGTCGG TTGCAGGGCG AAGGGATGGG GATGGGGAGG CCGAGCGGAG CTTGGTCGAC 120 CCCGTGGTTG GTGGTGGTTC TTGTGCATTG GCTCCTCTGG GCCACCGAGA GGAGGCGAGG 180
  • CCANCANAAA CAACTGCANA ACNTTNCNTN CCCAAGGATC TGTCCAACCT TACTCCCCNA 600
  • CNCNAACCCN AATACGGNTT GGCGCNGTCC CNGCACCCNC TGGAAACCCC TNGTNGGGAA 720 CNANTTGAAN CNGAAGGGGA AAGGCNTTCT TTNCCGAACA GGGAT 765
  • NANATCNCCC NCTGNTGATC TGGTGGNAAT CCCCCCCTTG TCCTGTGATT CTTGCTCTTG 360 ACNTTTTTCT CCCNTGTCNN ANCTCTCTNC CTNCTCTGGG TNTTGGGTCT TCCCCTGTTG 420
  • NCAGGGGAAG ACCATTACCT TGGAGGTGGA NANCTCNGAC ACGATTGATA ATGTCNAGGC 480 AAAGATCCAN GACAAGGANG GGATTCCACC GGATCAGCAN ANGCTGATCT TTGCTGGGAA 540 ACACTGGANG AAGGGCGCAC CCTGGCAGAT TNCNACATTC CNAAAGAATC CACCTTCACC 600
  • AAAGGAAATN AANCCNNTTG GGTTTTCCCC TTTGGCCTTT TTTTTGTTTG AAAATCCTNT 780
  • GGCACNANCC CGCTTCGGTA GCCATTATTG GTGCCCGTAG AGCGAGTGCT GAGATGGCTT 60
  • CCGTACCTCC TGCTCACAAG CCCCGAACTC CGCCGCCGGT TTACAAGTCT CCACCACCGA ⁇ 0 NCCACCGTCC TTACCCTCCA TCGACGCCAC CCCACCATCC GAAGCACCCG CCTTCTCAGC 540
  • GGGGTTTTTN TTAANATTTN
  • AANAAANCNA ANTNTTTTCC CCCCCNTNTT GGTTNATGGC 780 CTATGGGTTT GTTTCTCTTC TTGGCTTTTC CCGG 814
  • TCNTACCCAA CCCACCTAAG CTGCCTCCCG GTGGCCCGCC CGTGTTTCCC CTCCTTCAAN 540
  • TCCAGTGCCC TTGAGCTCCC CCTGCGGATC ACATCNTCGA TCCTCTTTGC CCGTGGTGAA 540 TTTTMTTGAG CATTCCTTTG TGTCTTTACT TCCCTTTGTT TTTGGCTTTT TGATGACGAA 600
  • CTATTCCGAC TCATCGAAAG TCGGCTATGT TCTTGAAGGA AGAGCGGTGG TGGGGCTAAC 240 ACTCTATGGA GAGACCGAGC AGAGGATACT GCTGCTTGAG AAGGGAGATG TGGTAGCGGT 300
  • TTCTCATCNA NATANCCGTA ATTAANGACT TCCGGTCATG CNANAATNCC CNACCNANGA 720
  • GTAACACCGT CAAGTACGAT TTCCCATCCT TCGGGTTGTG GCGTTCTGCT ACTTGGCATT 480
  • AACTCCCCCA TCTCCTGGGG CGTTGGCGAT GGACTCCGGC GAATGGTCCT CCTACCTCCC 540
  • CAAGTNACTA ACAGCAGTGG CTACTTGGTG CTTCNTTGGG GTGCAATTCA TAATANAAGG 540
  • TCTCANGANA CCTTTTAAAA AATCTGGTTC CCCATCNTCC GTNAAAATGG AGATTGATGA 660 TCCTGAAATA CCAGCAGTNG AATTTCTCCT TANTTTTGNA ACAAATNCTC AAAAAAACCN 720
  • GGCTCATGCG CTGGCTATGG GGGTGTCGTC GACTCGGAGC CAAGGACCTT CCTAAACAGA 180 AGGGTTCTAC ACTTGAGGAA CCAAACCACC GCTTACGAAG GATTGAGATC TCGAAATGTG 240
  • CTNTCCCCGG AACTGATTTT NAAAAACANC CCCTAAANTT TANCCTTTTN TNCCCNGCCN 720
  • NCCNAATACT TTTCCTGGAA CCATTTNGGG GANAANNTTG TTNTTTGTTG CNAATTAATT 780 GGCNCCCTGG TCCTCCTNCC TGCTACTTAA AAATTTTNTT TCNTNT 826
  • TTTTGTNCCN GCNNCTTTGG AACCNCCCNG GATCTNGATN TTANCACCCC CAANTCTTAT 780 CCTGGAACTT TTGGGGNAAA NTTTGTNTTT GTT 813
  • AAGAATTGAC AGGGTTTTCA TTGATCATCC TTTGTTTCTT GAAAAAGTGT GGGGAAAAAC 600 TGGGAAGAAT GATATNTGGT CCTGTCCCNG GAACTGATTA TAAAACACCA GCTAANATTA 660
  • TTTGTACATC ATCTTTATCG ACAACATCAC AATCAGGATT GAAGGGACCC ATATGAAACC 360
  • CTGTGATGCC TTCTATGACA GTGTCAGCAA GTCCACCAGT TGTTGCGCAC ATGGGGGGAA 420 TTCCATATCG CATGCCCTGA AAGCTGGATA AGGCCACAAG GTTCGAATCT GCTGGTAAGA 480
  • TCCCCTCAAA AGAACACCCG TGCGGCCCAA AATCCGGNCC CCCGGTGGTT CCCCCTTTCN 720 CTTTAANGGT CCCCAATGCC CCTGNTTTAA CTGNCTCCCC CCCANGCNCG GCCCCGGCCC 780
  • CTTAGCAGAT GCTTGTGCCA CAGGCAACTA CGAATACGTG AACATCGCCA CCCTTTTCAA 240
  • GATAGCCTCC TCATTCACTA TGGCGATCCG ATCGCCAGCT TCGCTGCTGT TATTTGCGTT 120
  • CTCCACAAAC CTTCNTGCAT TCTCCCCAAA CCTATCAATC TTGCNAAATG CGTTCAACAA 780 TTNGGGTCTT TTCATCCCCG CCCAAAACT 809

Abstract

Cette invention se rapporte à un procédé de modulation des caractéristiques de mûrissement et/ou de vieillissement des plantes du genre Musa. Ledit procédé consiste à transformer des plantes avec une ou plusieurs séquences qu'il est possible d'obtenir à partir de la banque génomique déposée de numéro d'ordre 40184, à régénérer lesdites plantes et à sélectionner dans la population de transformants les plantes qui présentent des caractéristiques modulées de mûrissement et/ou de vieillissement tissulaire.
EP97939069A 1996-09-10 1997-09-08 Modulation genetique du murissement de fruits Withdrawn EP0931150A2 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GBGB9618862.8A GB9618862D0 (en) 1996-09-10 1996-09-10 Genetic control of fruit ripening
GB9618862 1996-09-10
GBGB9708366.1A GB9708366D0 (en) 1997-04-25 1997-04-25 Genetic control of fruit ripening
GB9708366 1997-04-25
PCT/GB1997/002424 WO1998011228A2 (fr) 1996-09-10 1997-09-08 Modulation genetique du murissement de fruits

Publications (1)

Publication Number Publication Date
EP0931150A2 true EP0931150A2 (fr) 1999-07-28

Family

ID=26310006

Family Applications (1)

Application Number Title Priority Date Filing Date
EP97939069A Withdrawn EP0931150A2 (fr) 1996-09-10 1997-09-08 Modulation genetique du murissement de fruits

Country Status (5)

Country Link
EP (1) EP0931150A2 (fr)
JP (1) JP2000517164A (fr)
AU (1) AU4129197A (fr)
CA (1) CA2262505A1 (fr)
WO (1) WO1998011228A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9716766D0 (en) * 1997-08-07 1997-10-15 Innes John Centre Pectin degrading enzymes
CA2304109A1 (fr) 1997-09-25 1999-04-01 Boyce Thompson Institute For Plant Research, Inc. Proteines de banane, adn, et elements regulateurs d'adn associes au developpement du fruit
US6864404B1 (en) * 1999-10-15 2005-03-08 Carnegie Institution Of Washington Engineering disease resistance with pectate lyase-like genes
US20130036515A1 (en) * 2011-08-05 2013-02-07 Giovannoni James J Banana MADS-Box Genes for Banana Ripening Control

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08507923A (ja) * 1993-03-22 1996-08-27 ゼネカ・リミテッド Ｄｎａ、ｄｎａ構築物、細胞及びそれから誘導された植物
GB9318927D0 (en) * 1993-09-13 1993-10-27 Zeneca Ltd Regulation of senescence
DE69429012T2 (de) * 1993-12-09 2002-08-22 Texas A & M Univ Sys Transformation von musa-arten durch verwendung von agrobacterium tumefaciens

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO9811228A3 *

Also Published As

Publication number Publication date
WO1998011228A3 (fr) 1998-04-23
CA2262505A1 (fr) 1998-03-19
WO1998011228A2 (fr) 1998-03-19
JP2000517164A (ja) 2000-12-26
AU4129197A (en) 1998-04-02

Similar Documents

Publication Publication Date Title
US5908973A (en) DNA encoding fruit-ripening-related proteins, DNA constructs, cells and plants derived therefrom
Kobayashi et al. Myb-related genes of the Kyoho grape (Vitis labruscana) regulate anthocyanin biosynthesis
EP0686197B1 (fr) Systeme d'expression d'un gene a base d'un geminivirus
US6359197B1 (en) Transgenic plants with altered senescence characteristics
US7915481B2 (en) Maize ETR2 gene and uses thereof
US6388174B1 (en) Gene encoding α-subunit of rice anthranilate synthase and DNA relating thereto
EP1321525A2 (fr) Genes pour la maturation des fruites
US5650303A (en) Geminivirus-based gene expression system
JPH06503954A (ja) 植物ｄｎａ
WO1997013851A9 (fr) Amelioration de la qualite des fruits par inhibition de la production de lipoxygenase dans ces fruits
WO1997013851A1 (fr) Amelioration de la qualite des fruits par inhibition de la production de lipoxygenase dans ces fruits
US5545815A (en) Control of fruit ripening in plants
AU709862B2 (en) Transgenic plants expressing ACC synthase gene
WO1998011228A2 (fr) Modulation genetique du murissement de fruits
JP4095956B2 (ja) 種子発生期における胚／内乳のサイズの改変
US20020120960A1 (en) Genetic control of fruit ripening
WO1997021816A1 (fr) Modulation genetique du murissement des fruits
WO2006076717A2 (fr) Nouvelle cytochrome p450 monooxygenase
US5981236A (en) Geminivirus-based gene expression system
WO1998053085A1 (fr) Maturation des fruits par genie genetique
US20020026657A1 (en) Genetic control of fruit ripening
AU2013202733B2 (en) Manipulation of soluble carbohydrates (5)
CN112680472B (zh) ZmSPL基因在调控玉米冠根或气生根发育中的应用
WO2008064413A1 (fr) Plantes comportant des tissus accumulant l'hexose qui présentent une concentration d'hexose modifiée
AU2007237376B2 (en) Manipulation of soluble carbohydrates (2)

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 19990412

AK Designated contracting states

Kind code of ref document: A2

Designated state(s): AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: SYNGENTA LIMITED

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: SYNGENTA LIMITED

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION HAS BEEN WITHDRAWN

18W Application withdrawn

Effective date: 20040212