EP0855445A4 - Process for producing hydroxylated cholesterols by biological conversion and dihydroxycholesterols - Google Patents

Process for producing hydroxylated cholesterols by biological conversion and dihydroxycholesterols

Info

Publication number
EP0855445A4
EP0855445A4 EP96927894A EP96927894A EP0855445A4 EP 0855445 A4 EP0855445 A4 EP 0855445A4 EP 96927894 A EP96927894 A EP 96927894A EP 96927894 A EP96927894 A EP 96927894A EP 0855445 A4 EP0855445 A4 EP 0855445A4
Authority
EP
European Patent Office
Prior art keywords
dihydroxycholesterols
biological conversion
producing hydroxylated
cholesterols
hydroxylation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP96927894A
Other languages
German (de)
French (fr)
Other versions
EP0855445A1 (en
EP0855445B1 (en
Inventor
Koji Takeda
Tadashi Terasawa
Kazuyuki Dobashi
Takeo Yoshioka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mercian Corp
Original Assignee
Mercian Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mercian Corp filed Critical Mercian Corp
Publication of EP0855445A1 publication Critical patent/EP0855445A1/en
Publication of EP0855445A4 publication Critical patent/EP0855445A4/en
Application granted granted Critical
Publication of EP0855445B1 publication Critical patent/EP0855445B1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/06—Hydroxylating

Landscapes

  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Steroid Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A method for preparing at least one hydroxycholesterol chosen from the group consisting of 25-hydroxycholesterol, 17, 25-dihydroxycholesterol and 25, 26-dihydroxycholesterol by biological hydroxylation of cholesterol, and the aforementioned dihydroxycholesterols. In the above biological hydroxylation, a microorganism is used which has the abovementioned hydroxylation capacity and which belongs to the genus Amycolata and the genus Sphingomonas.
EP96927894A 1995-08-28 1996-08-26 Process for producing hydroxylated cholesterols by biological conversion and dihydroxycholesterols Expired - Lifetime EP0855445B1 (en)

Applications Claiming Priority (10)

Application Number Priority Date Filing Date Title
JP24046195 1995-08-28
JP24046195 1995-08-28
JP240461/95 1995-08-28
JP31975895 1995-11-15
JP31975895 1995-11-15
JP319758/95 1995-11-15
JP321250/95 1995-11-16
JP32125095 1995-11-16
JP32125095 1995-11-16
PCT/JP1996/002369 WO1997008336A1 (en) 1995-08-28 1996-08-26 Process for producing hydroxylated cholesterols by biological conversion and dihydroxycholesterols

Publications (3)

Publication Number Publication Date
EP0855445A1 EP0855445A1 (en) 1998-07-29
EP0855445A4 true EP0855445A4 (en) 1998-09-30
EP0855445B1 EP0855445B1 (en) 2003-03-12

Family

ID=27332829

Family Applications (1)

Application Number Title Priority Date Filing Date
EP96927894A Expired - Lifetime EP0855445B1 (en) 1995-08-28 1996-08-26 Process for producing hydroxylated cholesterols by biological conversion and dihydroxycholesterols

Country Status (8)

Country Link
US (2) US6146844A (en)
EP (1) EP0855445B1 (en)
JP (1) JP3742435B2 (en)
AT (1) ATE234363T1 (en)
AU (1) AU6754696A (en)
DE (1) DE69626656T2 (en)
DK (1) DK0855445T3 (en)
WO (1) WO1997008336A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0916736B1 (en) * 1996-06-27 2005-11-16 Mercian Corporation Biological process for preparing 25-hydroxylated steroids
US7556493B2 (en) * 2005-02-24 2009-07-07 Suttle James M Methods and apparatus for molding chocolate
CN111892637B (en) * 2020-07-16 2022-10-14 山东海能生物工程有限公司 Preparation method of 7-dehydro-25-hydroxycholesterol

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04356190A (en) * 1990-05-08 1992-12-09 Taisho Pharmaceut Co Ltd Production of vitamin ds
JPH07123997A (en) * 1993-11-02 1995-05-16 Chugai Pharmaceut Co Ltd Biological production of hydroxide at 25-position of steroids
EP0653490A1 (en) * 1992-06-25 1995-05-17 Taisho Pharmaceutical Co. Ltd Vitamin d hydroxylase gene

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4204995A (en) * 1978-03-15 1980-05-27 Hoffmann-La Roche Inc. Cholestane derivatives
JP3201411B2 (en) * 1990-06-15 2001-08-20 メルシャン株式会社 Biological production of vitamin Ds
JPH07241197A (en) * 1994-01-11 1995-09-19 Kyowa Hakko Kogyo Co Ltd Production of 25-hydroxyvitamin d

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04356190A (en) * 1990-05-08 1992-12-09 Taisho Pharmaceut Co Ltd Production of vitamin ds
EP0653490A1 (en) * 1992-06-25 1995-05-17 Taisho Pharmaceutical Co. Ltd Vitamin d hydroxylase gene
JPH07123997A (en) * 1993-11-02 1995-05-16 Chugai Pharmaceut Co Ltd Biological production of hydroxide at 25-position of steroids

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
BARNER R ET AL: "Configuration of the vitamin-D3-metabolite 25,26-dihydroxycholecalciferol: synthesis of (25S,26)- and (25R,26)-dihydroxycholecalciferol", HELVETICA CHIMICA ACTA., vol. 64, no. 3, 1981, BASEL CH, pages 915 - 938, XP002070164 *
CHEMICAL ABSTRACTS, vol. 118, no. 17, 26 April 1993, Columbus, Ohio, US; abstract no. 167619, SASAKI J ET AL: "Manufacture of vitamin D with Actinomycetes" page 746; XP002070166 *
DATABASE WPI Section Ch Week 9304, Derwent World Patents Index; Class B01, AN 93-030367, XP002070167 *
DATABASE WPI Section Ch Week 9528, Derwent World Patents Index; Class B01, AN 95-211636, XP002070168 *
ISHIGURO M ET AL: "Stereoselective introduction of hydroxy-groups into the 24-, 25-, and 26-positions of the cholesterol side chain", JOURNAL OF THE CHEMICAL SOCIETY, CHEMICAL COMMUNICATIONS., no. 3, 1981, LETCHWORTH GB, pages 115 - 117, XP002070165 *
KOIZUMI N ET AL: "Stereoselective introduction of hydroxy groups into the cholesterol side chain. Preparation of (24R)- and (24S)-24,25-dihydroxy- and (25R)- and (25S)-25,26-dihydroxyvitamin D3 by asymmetric synthesis", JOURNAL OF THE CHEMICAL SOCIETY, PERKIN TRANSACTIONS 1., no. 7, 1983, LETCHWORTH GB, pages 1401 - 1410, XP002070163 *
LAM H Y ET AL: "Synthesis and biological activity of 25.xi.,26-dihydroxycholecalciferol", STEROIDS., vol. 25, no. 2, February 1975 (1975-02-01), SAN FRANCISCO US, pages 247 - 256, XP002070161 *
PARTRIDGE J J ET AL: "Synthesis and structure proof of a vitamin D3 metabolite, 25(s),26-dihydroxycholecalciferol", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY., vol. 103, no. 5, 11 March 1981 (1981-03-11), DC US, pages 1253 - 1255, XP002070162 *

Also Published As

Publication number Publication date
US6146844A (en) 2000-11-14
EP0855445A1 (en) 1998-07-29
DE69626656D1 (en) 2003-04-17
AU6754696A (en) 1997-03-19
JP3742435B2 (en) 2006-02-01
WO1997008336A1 (en) 1997-03-06
DK0855445T3 (en) 2003-06-30
EP0855445B1 (en) 2003-03-12
US6410759B1 (en) 2002-06-25
DE69626656T2 (en) 2003-09-18
ATE234363T1 (en) 2003-03-15

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