EP0854931A4 - Coiled-coil heterodimer methods and compositions for the detection and purification of expressed proteins - Google Patents

Coiled-coil heterodimer methods and compositions for the detection and purification of expressed proteins

Info

Publication number
EP0854931A4
EP0854931A4 EP96934080A EP96934080A EP0854931A4 EP 0854931 A4 EP0854931 A4 EP 0854931A4 EP 96934080 A EP96934080 A EP 96934080A EP 96934080 A EP96934080 A EP 96934080A EP 0854931 A4 EP0854931 A4 EP 0854931A4
Authority
EP
European Patent Office
Prior art keywords
coiled
purification
compositions
detection
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP96934080A
Other languages
German (de)
French (fr)
Other versions
EP0854931A1 (en
Inventor
Michael E Houston Jr
Lei Yu
Daisy Bautista
Robert S Hodges
Brian Tripet
Paul J Cachia
Randall T Irvin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pence Inc
Original Assignee
Pence Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pence Inc filed Critical Pence Inc
Publication of EP0854931A1 publication Critical patent/EP0854931A1/en
Publication of EP0854931A4 publication Critical patent/EP0854931A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1203—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria
    • C07K16/1214—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria from Pseudomonadaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/73—Fusion polypeptide containing domain for protein-protein interaction containing coiled-coiled motif (leucine zippers)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
EP96934080A 1995-10-06 1996-10-04 Coiled-coil heterodimer methods and compositions for the detection and purification of expressed proteins Withdrawn EP0854931A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US54039795A 1995-10-06 1995-10-06
US540397 1995-10-06
PCT/US1996/016032 WO1997012988A1 (en) 1995-10-06 1996-10-04 Coiled-coil heterodimer methods and compositions for the detection and purification of expressed proteins

Publications (2)

Publication Number Publication Date
EP0854931A1 EP0854931A1 (en) 1998-07-29
EP0854931A4 true EP0854931A4 (en) 2002-02-13

Family

ID=24155285

Family Applications (1)

Application Number Title Priority Date Filing Date
EP96934080A Withdrawn EP0854931A4 (en) 1995-10-06 1996-10-04 Coiled-coil heterodimer methods and compositions for the detection and purification of expressed proteins

Country Status (4)

Country Link
EP (1) EP0854931A4 (en)
JP (1) JPH11512620A (en)
AU (1) AU695679B2 (en)
WO (1) WO1997012988A1 (en)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6271198B1 (en) 1996-11-06 2001-08-07 Genentech, Inc. Constrained helical peptides and methods of making same
GB9714681D0 (en) * 1997-07-11 1997-09-17 Medical Res Council Recombinant production of proteins
GB9714680D0 (en) 1997-07-11 1997-09-17 Medical Res Council Recombinant production of proteins
GB9718358D0 (en) * 1997-08-30 1997-11-05 Univ Leeds Chemical modification
US7105307B2 (en) 1997-08-30 2006-09-12 Cyclacel, Ltd. Compositions and methods for screening for modulators of enzymatic activity
DE19815331A1 (en) * 1998-04-06 1999-10-14 Karlsruhe Forschzent Transcription factors and their use
DE69906729T2 (en) 1998-06-12 2004-02-05 The Governors Of The University Of Alberta, Edmonton COMPOSITION AND METHOD FOR TREATING PSEUDOMONAS AERUGINOSA INFECTIONS
GB2341115B (en) * 1998-09-04 2002-02-06 Actinova Ltd Chromatography
US6380365B1 (en) * 1998-09-04 2002-04-30 Affitech As Temperature dependent ligand facilitated purification of proteins
WO2002067969A2 (en) * 2001-02-21 2002-09-06 Medtronic Minimed, Inc. Stabilized insulin formulations
GB0315993D0 (en) * 2003-07-08 2003-08-13 Sussex Ip Ltd Peptide-based linker
AU2004270774A1 (en) * 2003-09-05 2005-03-17 National Research Council Of Canada Coiled-coil fusion proteins comprising cell receptor domains
US7786261B2 (en) 2004-09-02 2010-08-31 National Research Council Of Canada Coiled-coil fusion proteins comprising cell receptor domains
US20120009212A1 (en) 2010-01-26 2012-01-12 The Regents Of The University Of Colorado, A Body Corporate Conjugates utilizing platform technology for stimulating immune response
JP5964300B2 (en) * 2010-08-02 2016-08-03 マクロジェニクス，インコーポレーテッド Covalently bonded diabody and its use
EP3873919A1 (en) * 2018-11-02 2021-09-08 University of Washington Orthogonal protein heterodimers

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0491362A2 (en) * 1990-12-19 1992-06-24 BEHRINGWERKE Aktiengesellschaft Use of peptide pairs with extremely high mutual specific affinity in the domain of in-vitro diagnostics
WO1995031480A1 (en) * 1994-05-18 1995-11-23 S.P.I. Synthetic Peptides Incorporated Heterodimer polypeptide immunogen carrier composition and method

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0491362A2 (en) * 1990-12-19 1992-06-24 BEHRINGWERKE Aktiengesellschaft Use of peptide pairs with extremely high mutual specific affinity in the domain of in-vitro diagnostics
WO1995031480A1 (en) * 1994-05-18 1995-11-23 S.P.I. Synthetic Peptides Incorporated Heterodimer polypeptide immunogen carrier composition and method

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
GRADDIS TJ ET AL.: "Controlled formation of model homo- and heterodimer coiled coil polypeptides", BIOCHEMISTRY., vol. 32, 1993, AMERICAN CHEMICAL SOCIETY. EASTON, PA., US, pages 12664 - 12671, XP001041686, ISSN: 0006-2960 *
HODGES R S: "DE NOVO DESIGN OF ALPHA-HELICAL PROTEINS: BASIC RESEARCH TO MEDICALAPPLICATIONS", BIOCHEMISTRY AND CELL BIOLOGY. BIOCHIMIE ET BIOLOGIE CELLULAIRE, XX, XX, vol. 74, no. 2, 17 June 1995 (1995-06-17), pages 133 - 154, XP002185479, ISSN: 0829-8211 *
See also references of WO9712988A1 *

Also Published As

Publication number Publication date
WO1997012988A1 (en) 1997-04-10
EP0854931A1 (en) 1998-07-29
AU695679B2 (en) 1998-08-20
AU7258496A (en) 1997-04-28
JPH11512620A (en) 1999-11-02

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