EP0820348A1 - Analysis card - Google Patents

Analysis card

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Publication number
EP0820348A1
EP0820348A1 EP97901736A EP97901736A EP0820348A1 EP 0820348 A1 EP0820348 A1 EP 0820348A1 EP 97901736 A EP97901736 A EP 97901736A EP 97901736 A EP97901736 A EP 97901736A EP 0820348 A1 EP0820348 A1 EP 0820348A1
Authority
EP
European Patent Office
Prior art keywords
chambers
partition
card
card according
cavities
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP97901736A
Other languages
German (de)
French (fr)
Other versions
EP0820348B1 (en
Inventor
Bernard Jean-Marie Limon
Fabienne Marquis-Weible
Philippe Renaud
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biomerieux SA
Original Assignee
Biomerieux SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomerieux SA filed Critical Biomerieux SA
Publication of EP0820348A1 publication Critical patent/EP0820348A1/en
Application granted granted Critical
Publication of EP0820348B1 publication Critical patent/EP0820348B1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2575—Volumetric liquid transfer

Definitions

  • the present invention relates to the processing of an analysis card.
  • analysis is meant any process or process making it possible to identify, separate, isolate, determine, detect, or quantify, a material, a product, a substance, or a compound, designated under the generic expression of “analyte ", from a sample or sample to be analyzed, previously diluted or not with any suitable medium, for example a solvent.
  • the analyte sought may be of a chemical, biochemical or even biological nature, for example in the latter case an antigen or an antibody.
  • analysis card any device, module, or system, arranged internally to carry out the various processes or reactions necessary for the identification, separation, detection or quantification of the analyte, by means of different processing, in particular within said card, or manipulation of the analysis card, for example automatically.
  • Such an analysis card well known to those skilled in the art, constitutes a closed assembly vis-à-vis the outside or its immediate environment, with the exception of course of any passages or equivalent means, allowing in particular and initially to introduce the sample or sample to be analyzed.
  • Such an analysis card contains the reagent (s), physico-chemical, chemical, biochemical, or biological, distributed and maintained in the card, according to the path of the sample to be analyzed, and the reaction processes or reactions to be carried out to accomplish analysis.
  • An analysis card as envisaged by the present invention generally constituting a device or assembly for single use, that is to say discarded or destroyed after its use, comprises or integrates within it a plurality of chambers, arranged 97/28899 PCMB97 / 00112
  • analysis cards in series and / or in parallel, one of which, for example the last, is in particular an optical measurement chamber.
  • These analysis cards can be produced or produced by any suitable technique, for example in one or more parts assembled (for example welding) together, produced for example by molding one or more identical or different plastics.
  • chamber is meant in the claims and description any enclosure, or passage, ensuring the reception and / or circulation of any liquid, fluid, or gas present in the analysis card.
  • the present invention relates to a particular analysis card, allowing an operation of connecting at least two chambers, formed in an analysis card, and initially isolated from one another, this operation being carried out remotely and without mechanical action or contact with the analysis card.
  • the present invention lies in the cooperation of two essential means, namely, on the one hand, a light beam, in particular coherent, having at least a predetermined wavelength ⁇ , and a predetermined power P, obtained from a light source, in particular a laser, and on the other hand a structure or arrangement of an analysis card, in which are formed at least two chambers isolated from one another, and from the 'exterior; but this structure is specifically adapted to the implementation of this light beam, to establish at least one passage between at least two chambers of said card.
  • the two chambers are separated from each other by a partition, perforable, disposed within the card, of absorbent material, in particular plastic, absorbing the light energy of the light beam above, to transform it into thermal energy capable of eliminating at least locally said material; and two cavities are provided on either side of the partition, and are in communication with or included in the two chambers respectively; and a window made of a transparent material at least for the wavelength ⁇ is arranged opposite said partition, and delimits with the latter one of said cavities, called the incidence of a light ray.
  • An analysis card in accordance with the present invention makes it possible to implement the following method, and more particularly the following steps, cooperating with each other:
  • the light source is controlled or controlled, so that the thermal energy dissipated within the perforable partition does not exceed that necessary for the local elimination of the absorbent material, for example by fusion, vaporization, or sublimation, according to a perforating hole right through this partition, of limited or controlled radial extension.
  • the light beam used according to the invention can be convergent, parallel, or divergent, the essential condition being the power density of the light ray striking the perforable partition.
  • suitable guiding means can be provided before and / or after the beam, to satisfy the essential condition mentioned above.
  • US-C-5 411 065 it has already been proposed to use a laser light beam to remotely perforate a wall.
  • the method according to the present invention therefore allows manipulation of the analysis card, in particular by automatic means, leading, in relation to the light beam, in particular laser, to the perforation of any partition, disposed within said card, and this while preserving the rest or the integrity of the latter.
  • Such a method therefore appears to be particularly suitable for today's analysis apparatus, working for a large part, if not entirely, automatically.
  • FIG. 1 shows, schematically, a step in the method of processing an analysis card, according to the invention
  • FIG. 2 shows this same analysis card, as obtained after the step or operation shown diagrammatically in Figure 1;
  • - Figures 3 to 5 show respectively, still schematically, three successive steps of a treatment method according to the invention, implemented with an analysis card produced according to another embodiment of the invention;
  • - Figure 6 shows, schematically, a device for processing an analysis card, according to the invention.
  • the analysis card 1 shown is intended to cooperate with or adapted to a laser light source 4, emitting a coherent light or light beam 5, having a predetermined wavelength ⁇ , and a predetermined light power P.
  • the analysis card 1 has a substantially flattened general shape, and comprises, in an assembled manner, for example by gluing, a flat body 14, for example a polystyrene or polycarbonate wafer, previously engraved or imprinted, as described below, between two external walls, or films 15 and 16, for example of transparent plastic vis-à-vis the light beam.
  • the films 15 and 16 can be made of polypropylene, silicon or germanium.
  • two chambers 2 and 3 are obtained and included within the analysis card 1, being, on the one hand each isolated from the outside of the card, and on the other hand isolated initially one relative to the other.
  • the two chambers 2 and 3 are separated from each other by a partition 7 which can be pierced by the laser light from the source 4, made of absorbent material, in particular plastic, absorbing light energy of length d wave ⁇ of the laser source, in order to transform it into thermal or calorific energy capable of eliminating or making disappear at least locally or punctually the material of the partition 7.
  • Two cavities 8 and 9 are formed on either side of the partition 7, and are respectively in communication with the two chambers 2 and 3.
  • the arrangements described above are obtained in the following manner: - in the body 14, the two chambers 2 and 3 are imprinted or engraved, as well as the two cavities 8 and 9; and the external walls 15 and 16 close these chambers and cavities with respect to the outside;
  • the chamber 2 is located below the cavity 8, and the chamber 3 is located above the cavity 9; one 2 of the chambers and the cavity 8 which corresponds to it are closed by one of the external walls, and the other chamber 3 and the other cavity 9 which corresponds to it, are closed by the other external wall 16;
  • the perforable partition 7 is formed in the flat body 14, and therefore in its constituent material which is absorbing light energy of wavelength ⁇ ; -
  • the two windows 10 and 11 are formed in the external walls 15 and 16 respectively, of which the constituent material is, as said above, transparent for the wavelength ⁇ .
  • the processing method according to the invention comprises the following steps:
  • the analysis card 1 has the structure and arrangement described above, characterized mainly by the partition 7, disposed within the card 1, capable of receiving light from the source 4, through window 10, in the form of an incident light ray 12; (c) the analysis card 1 is placed with respect to the light beam 5, so that the incidence of the light ray 12 illuminating the partition 7 is substantially perpendicular to the latter; (d) the laser light source 4 is controlled or controlled, so that the thermal energy dissipated within the partition 7 does not exceed that necessary for the local elimination or disappearance of the absorbent material constituting the partition 7, according to a hole 7a (see Figure 2) perforating the partition 7, of limited radial extension, that is to say just sufficient to establish communication or full passage between the cavities 8 and 9, and therefore between the chambers 2 and 3.
  • the incidence of the light ray 12 illuminating the partition 7 is at the same time substantially perpendicular to the plane of the analysis card 1, and corresponds to the reference direction 6 of the light beam 5 emitted by the source 4.
  • the axis of the light ray 12 illuminating the partition 7 crosses the latter substantially at its center, providing an edge on either side of the perforating hole 7a, not affected by the light energy of the laser beam.
  • the light ray 12 illuminating the partition 7 is convergent according to a focal point 13 disposed in the center or within the partition 7.
  • the light ray 12 can also be, or parallel, or divergent, for the purposes of the illumination of the partition 7, according to the distribution of the light energy, desired in the impact zone of the light ray 12.
  • the movement of the liquid present in the chamber 3 is controlled, for example by capillarity and / or suction, so that the cavity 9, on the other side of the cavity 8 incidence relative to the perforable partition 7, remains full or empty of any liquid.
  • a cavity 9 remaining empty of any liquid at the time of illumination with the light beam 5 makes it possible in particular to preserve the liquid or the liquids circulating in the analysis card, from any untimely or excessive heating.
  • FIG. 3 to 5 differs from that shown in Figures 1 to 2, in that the analysis card 1 comprises "n", in this case 3 chambers 2, 3, 17, arranged in series , but can also be arranged in parallel, separated two by two by "n-1", in this case two perforable partitions 7 and 18, "n” being an integer, and one of the chambers 2,3 and 17 possibly including an optical measurement chamber.
  • the analysis card 1 comprises "n", in this case 3 chambers 2, 3, 17, arranged in series , but can also be arranged in parallel, separated two by two by "n-1", in this case two perforable partitions 7 and 18, "n” being an integer, and one of the chambers 2,3 and 17 possibly including an optical measurement chamber.
  • the perforable partitions 7 and 18 are distributed in the card 1, so that two partitions cannot be aligned with a single incident light beam 12
  • the analysis card of Figures 3 to 5 includes a liquid filling the chamber 3, which will flow towards the chamber 2, after perforation of the partition 7, as shown in Figure 4, then towards the chamber 17 , after perforation of the partition 18, as partially shown in Figure 5.
  • a device 19 for processing an analysis card 1 comprises: - a generator 20 of coherent light, having the predetermined wavelength ⁇ and the light power P, comprising a laser source, and means 25 for collimating and shaping the light beam emitted by the laser source; a beam of limited power, for example 2 mW, is emitted by a pointer in visible light and superimposed on the laser beam for its guidance;
  • Optical means 29 for coupling the quasi-parallel light beam, to inject the latter into an optical fiber 21, or optical guide means such as a prism or lens, with good efficiency;
  • - optical means 30 for collimating and / or shaping the beam, making it possible to obtain a circular "spot" of diameter and power density, determined on the work surface where the drilling will be carried out, located on or in each partition 7 or 18;
  • the generator 20 emits light at 800 nm, with guide light at 670 nm.
  • the power of the light source thus constituted is
  • the optical fiber 21 used is a multimode fiber with a core diameter of 200 ⁇ m.
  • a lens 30 makes it possible to focus the light energy on the center 13, located on or in each partition 7 or 18.
  • - partition 7 or 18 made of polystyrene, having a thickness of 0.3 mm;
  • the present invention can be implemented according to different embodiments; in particular one of the chambers, placed in communication according to the present invention, can itself communicate with the atmosphere or the environment in which the analysis card is located.

Abstract

PCT No. PCT/IB97/00112 Sec. 371 Date Nov. 14, 1997 Sec. 102(e) Date Nov. 14, 1997 PCT Filed Feb. 12, 1997 PCT Pub. No. WO97/28899 PCT Pub. Date Aug. 14, 1997An analysis card containing two mutually separate chambers separated by a frangible partition that is arranged within the card and made of an absorbent and preferably plastic material for absorbing light energy having at least a predetermined wavelength, and converting it into heat energy capable of at least locally removing the material.

Description

CARTE D'ANALYSE ANALYSIS CARD
La présente invention concerne le traitement d'une carte d'analyse.The present invention relates to the processing of an analysis card.
Par "analyse", on entend tout procédé ou processus permettant d'identifier, séparer, isoler, déterminer, détecter, ou quantifier, une matière, un produit, une substance, ou un composé, désigné sous l'expression générique d' "analyte", à partir d'un échantillon ou prélèvement à analyser, préalablement dilué ou non avec tout milieu approprié, par exemple un solvant. L'analyte recherché peut être de nature chimique, biochimique, ou encore biologique, par exemple dans ce dernier cas un antigène ou un anticorps.By "analysis" is meant any process or process making it possible to identify, separate, isolate, determine, detect, or quantify, a material, a product, a substance, or a compound, designated under the generic expression of "analyte ", from a sample or sample to be analyzed, previously diluted or not with any suitable medium, for example a solvent. The analyte sought may be of a chemical, biochemical or even biological nature, for example in the latter case an antigen or an antibody.
Par "carte d'analyse", on entend tout dispositif, module, ou système, agencé de manière interne pour effectuer les différents processus ou réactions nécessaires à l'identification, la séparation, la détection ou la quantification de l'analyte, moyennant différents traitements, en particulier au sein de ladite carte, ou manipulations de la carte d'analyse, par exemple par voie automatique. Une telle carte d'analyse, bien connue de l'homme de métier, constitue un ensemble fermé vis-à-vis de l'extérieur ou de son environnement immédiat, à l'exception bien entendu de tous passages ou moyens équivalents, permettant notamment et initialement d'introduire l'échantillon ou prélèvement à analyser. Une telle carte d'analyse contient le ou les réactifs, physico-chimiques, chimiques, biochimiques, ou biologiques, distribués et maintenus dans la carte, selon le cheminement de l'échantillon à analyser, et les processus réactionnels ou réactions à effectuer pour accomplir l'analyse. Une carte d'analyse telle qu'envisagée par la présente invention, constituant en général un dispositif ou ensemble à usage unique, c'est-à-dire jeté ou détruit après son emploi, comprend ou intègre en son sein une pluralité de chambres, disposées 97/28899 PCMB97/00112By "analysis card" is meant any device, module, or system, arranged internally to carry out the various processes or reactions necessary for the identification, separation, detection or quantification of the analyte, by means of different processing, in particular within said card, or manipulation of the analysis card, for example automatically. Such an analysis card, well known to those skilled in the art, constitutes a closed assembly vis-à-vis the outside or its immediate environment, with the exception of course of any passages or equivalent means, allowing in particular and initially to introduce the sample or sample to be analyzed. Such an analysis card contains the reagent (s), physico-chemical, chemical, biochemical, or biological, distributed and maintained in the card, according to the path of the sample to be analyzed, and the reaction processes or reactions to be carried out to accomplish analysis. An analysis card as envisaged by the present invention, generally constituting a device or assembly for single use, that is to say discarded or destroyed after its use, comprises or integrates within it a plurality of chambers, arranged 97/28899 PCMB97 / 00112
en série et/ou en parallèle, dont l'une, par exemple la dernière, est notamment une chambre de mesure optique. Ces cartes d'analyse peuvent être réalisées ou produites selon toutes techniques appropriées, par exemple en une ou plusieurs pièces assemblées (par exemple soudage) les unes aux autres, réalisées par exemple par moulage d'une ou plusieurs matières plastiques identiques ou différentes.in series and / or in parallel, one of which, for example the last, is in particular an optical measurement chamber. These analysis cards can be produced or produced by any suitable technique, for example in one or more parts assembled (for example welding) together, produced for example by molding one or more identical or different plastics.
Par "chambre", on désigne dans les revendications et la description toute enceinte, ou passage, assurant la réception et/ou la circulation de tout liquide, fluide, ou gaz présent dans la carte d'analyse.By "chamber" is meant in the claims and description any enclosure, or passage, ensuring the reception and / or circulation of any liquid, fluid, or gas present in the analysis card.
La présente invention a pour objet une carte d'analyse particulière, permettant une opération de mise en communication d'au moins deux chambres, ménagées dans une carte d'analyse, et au départ isolées l'une par rapport à l'autre, cette opération étant effectuée à distance et sans action ou contact mécanique avec la carte d'analyse.The present invention relates to a particular analysis card, allowing an operation of connecting at least two chambers, formed in an analysis card, and initially isolated from one another, this operation being carried out remotely and without mechanical action or contact with the analysis card.
La présente invention réside dans la coopération de deux moyens essentiels, à savoir, d'une part d'un faisceau lumineux, notamment cohérent, ayant au moins une longueur d'onde prédéterminée λ, et une puissance prédéterminée P, obtenu à partir d'une source lumineuse, notamment laser, et d'autre part d'une structure ou agencement d'une carte d'analyse, dans laquelle sont ménagées au moins deux chambres isolées l'une par rapport à l'autre, et par rapport à l'extérieur ; mais cette structure est spécifiquement adaptée à la mise en oeuvre de ce faisceau lumineux, pour établir au moins un passage entre deux chambres au moins de ladite carte.The present invention lies in the cooperation of two essential means, namely, on the one hand, a light beam, in particular coherent, having at least a predetermined wavelength λ, and a predetermined power P, obtained from a light source, in particular a laser, and on the other hand a structure or arrangement of an analysis card, in which are formed at least two chambers isolated from one another, and from the 'exterior; but this structure is specifically adapted to the implementation of this light beam, to establish at least one passage between at least two chambers of said card.
Plus précisément, selon la présente invention, les deux chambres sont séparées l'une de l'autre par une cloison, perforable, disposée au sein de la carte, en matériau absorbant, notamment en matière plastique, absorbant l'énergie lumineuse du faisceau lumineux précité, pour la transformer en une énergie thermique susceptible d'éliminer au moins localement ledit matériau ; et deux cavités sont ménagées de part et d'autre de la cloison, et sont en communication avec ou comprises dans respectivement les deux chambres ; et une fenêtre en un matériau transparent au moins pour la longueur d'onde λ est disposée en vis-à-vis de ladite cloison, et délimite avec cette dernière l'une desdites cavités, dite d'incidence d'un rayon lumineux.More specifically, according to the present invention, the two chambers are separated from each other by a partition, perforable, disposed within the card, of absorbent material, in particular plastic, absorbing the light energy of the light beam above, to transform it into thermal energy capable of eliminating at least locally said material; and two cavities are provided on either side of the partition, and are in communication with or included in the two chambers respectively; and a window made of a transparent material at least for the wavelength λ is arranged opposite said partition, and delimits with the latter one of said cavities, called the incidence of a light ray.
Une carte d'analyse conforme à la présente invention permet de mettre en oeuvre le procédé suivant, et plus particulièrement les étapes suivantes, coopérant les unes avec les autres :An analysis card in accordance with the present invention makes it possible to implement the following method, and more particularly the following steps, cooperating with each other:
(a) à partir de la source lumineuse, notamment laser, on dispose du faisceau lumineux précité, ayant une puissance lumineuse prédéterminée P ;(A) from the light source, in particular laser, there is available the aforementioned light beam, having a predetermined light power P;
(b) la carte d'analyse est agencée comme défini précédemment ;(b) the analysis card is arranged as defined above;
(c) on dispose la carte d'analyse par rapport au faisceau lumineux, en sorte que l'incidence du rayon lumineux illuminant la cloison perforable soit sensiblement perpendiculaire à cette dernière ;(c) the analysis card is placed in relation to the light beam, so that the incidence of the light ray illuminating the perforable partition is substantially perpendicular to the latter;
(d) on contrôle ou commande la source lumineuse, en sorte que l'énergie thermique dissipée au sein de la cloison perforable n'excède pas celle nécessaire à l'élimination locale du matériau absorbant, par exemple par fusion, vaporisation, ou sublimation, selon un trou perforant de part en part cette cloison, d'extension radiale limitée ou contrôlée.(d) the light source is controlled or controlled, so that the thermal energy dissipated within the perforable partition does not exceed that necessary for the local elimination of the absorbent material, for example by fusion, vaporization, or sublimation, according to a perforating hole right through this partition, of limited or controlled radial extension.
Il est bien entendu que le faisceau lumineux utilisé selon l'invention peut être convergent, parallèle, ou divergent, la condition essentielle étant la densité de puissance du rayon lumineux frappant la cloison perforable. Quand le faisceau lumineux est parallèle ou divergent, des moyens de guidage appropriés peuvent être prévus avant et/ou après le faisceau, pour satisfaire la condition essentielle précitée. Comme décrit dans le document US-C-5 411 065, on a déjà proposé d'utiliser un faisceau lumineux laser pour perforer à distance une paroi. Cependant, jusqu'à présent, faute d'un agencement particulier conforme à la présente invention, il n'avait pas été possible d'utiliser le même moyen pour établir une communication contrôlée entre deux chambres au sein d'une même carte d'analyse.It is understood that the light beam used according to the invention can be convergent, parallel, or divergent, the essential condition being the power density of the light ray striking the perforable partition. When the light beam is parallel or divergent, suitable guiding means can be provided before and / or after the beam, to satisfy the essential condition mentioned above. As described in document US-C-5 411 065, it has already been proposed to use a laser light beam to remotely perforate a wall. However, until now, for lack of a particular arrangement in accordance with the present invention, it has not been possible to use the same means to establish a controlled communication between two chambers within the same analysis card. .
Le procédé conforme à la présente invention permet donc une manipulation de la carte d'analyse, notamment par voie automatique, conduisant, en relation avec le faisceau lumineux, notamment laser, à la perforation de toute cloison, disposée au sein de ladite carte, et ceci en préservant le reste ou l'intégrité de cette dernière. Un tel procédé apparaît donc particulièrement adapté aux appareils d'analyse d'aujourd'hui, travaillant pour une large part, sinon en totalité, de manière automatique.The method according to the present invention therefore allows manipulation of the analysis card, in particular by automatic means, leading, in relation to the light beam, in particular laser, to the perforation of any partition, disposed within said card, and this while preserving the rest or the integrity of the latter. Such a method therefore appears to be particularly suitable for today's analysis apparatus, working for a large part, if not entirely, automatically.
La présente invention est maintenant décrite par référence au dessin annexé, dans lequel :The present invention is now described with reference to the accompanying drawing, in which:
- la Figure 1 représente, de manière schématique, une étape du procédé de traitement d'une carte d'analyse, selon l'invention ;- Figure 1 shows, schematically, a step in the method of processing an analysis card, according to the invention;
- la Figure 2 représente cette même carte d'analyse, telle qu'obtenue après l'étape ou opération représentée schematiquement à la Figure 1 ; - les Figures 3 à 5 représentent respectivement, toujours de manière schématique, trois étapes successives d'un procédé de traitement selon l'invention, mises en oeuvre avec une carte d'analyse réalisée selon un autre mode d'exécution de l'invention ; - la Figure 6 représente, de manière schématique, un dispositif de traitement d'une carte d'analyse, selon l'invention.- Figure 2 shows this same analysis card, as obtained after the step or operation shown diagrammatically in Figure 1; - Figures 3 to 5 show respectively, still schematically, three successive steps of a treatment method according to the invention, implemented with an analysis card produced according to another embodiment of the invention; - Figure 6 shows, schematically, a device for processing an analysis card, according to the invention.
Conformément aux représentations des Figures 1 et 2, la carte d'analyse 1 représentée est destinée à coopérer avec ou adaptée à une source 4 lumineuse laser, émettant une lumière ou faisceau lumineux 5 cohérent, ayant une longueur d'onde prédéterminée λ, et une puissance lumineuse prédéterminée P .In accordance with the representations of FIGS. 1 and 2, the analysis card 1 shown is intended to cooperate with or adapted to a laser light source 4, emitting a coherent light or light beam 5, having a predetermined wavelength λ, and a predetermined light power P.
La carte d'analyse 1 a une forme générale substantiellement aplatie, et comprend, de manière assemblée, par exemple par collage, un corps plat 14, par exemple galette en polystyrène ou polycarbonate, préalablement gravé ou empreint, comme décrit ci-après, entre deux parois externes, ou films 15 et 16, par exemple en matière plastique transparente vis-à-vis du faisceau lumineux. Notamment les films 15 et 16 peuvent être en polypropylene, silicium ou germanium.The analysis card 1 has a substantially flattened general shape, and comprises, in an assembled manner, for example by gluing, a flat body 14, for example a polystyrene or polycarbonate wafer, previously engraved or imprinted, as described below, between two external walls, or films 15 and 16, for example of transparent plastic vis-à-vis the light beam. In particular, the films 15 and 16 can be made of polypropylene, silicon or germanium.
Comme décrit ci-après, deux chambres 2 et 3 sont obtenues et comprises au sein de la carte d'analyse 1, en étant, d'une part isolées chacune par rapport à l'extérieur de la carte, et d'autre part isolées au départ l'une par rapport l'autre. A cette fin, les deux chambres 2 et 3 sont séparées l'une de l'autre par une cloison 7 perforable par la lumière laser de la source 4, en matériau absorbant, notamment en matière plastique, absorbant l'énergie lumineuse de longueur d'onde λ de la source laser, pour la transformer en une énergie thermique ou calorifique susceptible d'éliminer ou faire disparaître au moins localement ou ponctuellement le matériau de la cloison 7. Deux cavités 8 et 9 sont ménagées de part et d'autre de la cloison 7, et sont respectivement en communication avec les deux chambres 2 et 3. Deux fenêtres 10 et 11, obtenues en un matériau transparent pour la longueur d'onde λ de la lumière laser de la source 4, sont disposées de part et d'autre et en vis-à-vis de la cloison 7, et délimitent avec cette dernière les deux cavités 8 et 9, l'une 8 servant à l'incidence d'un rayon lumineux identique ou issu du faisceau lumineux 5 provenant de la source 4, et l'autre servant à l'émergence du rayon lumineux, après perforation de la cloison 7. En pratique, compte tenu du mode de construction retenu pour la carte d'analyse 1, les dispositions précédemment décrites sont obtenues de la manière suivante : - dans le corps 14, sont empreintes ou gravées les deux chambres 2 et 3, ainsi que les deux cavités 8 et 9 ; et les parois externes 15 et 16 ferment ces chambres et cavités vis-à-vis de l'extérieur ;As described below, two chambers 2 and 3 are obtained and included within the analysis card 1, being, on the one hand each isolated from the outside of the card, and on the other hand isolated initially one relative to the other. To this end, the two chambers 2 and 3 are separated from each other by a partition 7 which can be pierced by the laser light from the source 4, made of absorbent material, in particular plastic, absorbing light energy of length d wave λ of the laser source, in order to transform it into thermal or calorific energy capable of eliminating or making disappear at least locally or punctually the material of the partition 7. Two cavities 8 and 9 are formed on either side of the partition 7, and are respectively in communication with the two chambers 2 and 3. Two windows 10 and 11, obtained in a material transparent for the wavelength λ of the laser light from the source 4, are arranged on both sides and d other and facing the partition 7, and delimit with the latter the two cavities 8 and 9, one 8 serving for the incidence of an identical light ray or coming from the light beam 5 coming from the source 4, and the other used for the emergence of light beam, after perforation of the partition 7. In practice, taking into account the construction method used for the analysis card 1, the arrangements described above are obtained in the following manner: - in the body 14, the two chambers 2 and 3 are imprinted or engraved, as well as the two cavities 8 and 9; and the external walls 15 and 16 close these chambers and cavities with respect to the outside;
- comme représenté aux figures 1 et 2, et dans la position horizontale de la carte d'analyse, la chambre 2 est située au-dessous de la cavité 8, et la chambre 3 est située au-dessus de la cavité 9 ; l'une 2 des chambres et la cavité 8 qui lui correspond sont fermées par l'une 15 des parois externes, et l'autre chambre 3 et l'autre cavité 9 qui lui correspond, sont fermées par l'autre paroi externe 16 ;- As shown in Figures 1 and 2, and in the horizontal position of the analysis card, the chamber 2 is located below the cavity 8, and the chamber 3 is located above the cavity 9; one 2 of the chambers and the cavity 8 which corresponds to it are closed by one of the external walls, and the other chamber 3 and the other cavity 9 which corresponds to it, are closed by the other external wall 16;
- la cloison perforable 7 est formée dans le corps plat 14, et donc dans son matériau constitutif qui est absorbant de l'énergie lumineuse de longueur d'onde λ ; - les deux fenêtres 10 et 11 sont formées dans les parois externes 15 et 16 respectivement, dont le matériau constitutif est, comme dit précédemment, transparent pour la longueur d'onde λ.- The perforable partition 7 is formed in the flat body 14, and therefore in its constituent material which is absorbing light energy of wavelength λ; - The two windows 10 and 11 are formed in the external walls 15 and 16 respectively, of which the constituent material is, as said above, transparent for the wavelength λ.
Avec la carte d'analyse précédemment décrite, le procédé de traitement selon l'invention comporte les étapes suivantes :With the previously described analysis card, the processing method according to the invention comprises the following steps:
(a) à partir de la source lumineuse 4 à laser, on dispose d'un faisceau lumineux 5 cohérent, ayant la longueur d'onde prédéterminée λ, et la puissance lumineuse P ; (b) la carte 1 d'analyse présente la structure et l'agencement décrits précédemment, se caractérisant principalement par la cloison 7, disposée au sein de la carte 1, susceptible de recevoir la lumière en provenance de la source 4, au travers de la fenêtre 10, sous la forme d'un rayon lumineux 12 incident ; (c) on dispose la carte d'analyse 1 par rapport au faisceau lumineux 5, en sorte que l'incidence du rayon lumineux 12 illuminant la cloison 7 soit sensiblement perpendiculaire à cette dernière ; (d) on contrôle ou commande la source lumineuse laser 4, en sorte que l'énergie thermique dissipée au sein de la cloison 7 n'excède pas celle nécessaire à l'élimination ou disparition locale du matériau absorbant, constitutif de la cloison 7, selon un trou 7a (cf Figure 2) perforant la cloison 7, d'extension radiale limitée, c'est-à-dire juste suffisante pour établir une communication ou plein passage entre les cavités 8 et 9, et par conséquent entre les chambres 2 et 3. Comme montré à la Figure 1, l'incidence du rayon lumineux 12 illuminant la cloison 7 est en même temps sensiblement perpendiculaire au plan de la carte 1 d'analyse, et correspond à la direction de référence 6 du faisceau lumineux 5 émis par la source 4. L'axe du rayon lumineux 12 illuminant la cloison 7 traverse cette dernière sensiblement en son centre, en ménageant une bordure de part et d'autre du trou perforant 7a, non affectée par l'énergie lumineuse du rayon laser. Préférentiellement, le rayon lumineux 12 illuminant la cloison 7 est convergent selon un point focal 13 disposé au centre ou au sein de la cloison 7. Mais le rayon lumineux 12 peut être aussi, ou parallèle, ou divergent, aux fins de l'illumination de la cloison 7, selon la répartition de l'énergie lumineuse, désirée dans la zone d'impact du rayon lumineux 12.(a) from the laser light source 4, there is a coherent light beam 5, having the predetermined wavelength λ, and the light power P; (b) the analysis card 1 has the structure and arrangement described above, characterized mainly by the partition 7, disposed within the card 1, capable of receiving light from the source 4, through window 10, in the form of an incident light ray 12; (c) the analysis card 1 is placed with respect to the light beam 5, so that the incidence of the light ray 12 illuminating the partition 7 is substantially perpendicular to the latter; (d) the laser light source 4 is controlled or controlled, so that the thermal energy dissipated within the partition 7 does not exceed that necessary for the local elimination or disappearance of the absorbent material constituting the partition 7, according to a hole 7a (see Figure 2) perforating the partition 7, of limited radial extension, that is to say just sufficient to establish communication or full passage between the cavities 8 and 9, and therefore between the chambers 2 and 3. As shown in Figure 1, the incidence of the light ray 12 illuminating the partition 7 is at the same time substantially perpendicular to the plane of the analysis card 1, and corresponds to the reference direction 6 of the light beam 5 emitted by the source 4. The axis of the light ray 12 illuminating the partition 7 crosses the latter substantially at its center, providing an edge on either side of the perforating hole 7a, not affected by the light energy of the laser beam. Preferably, the light ray 12 illuminating the partition 7 is convergent according to a focal point 13 disposed in the center or within the partition 7. But the light ray 12 can also be, or parallel, or divergent, for the purposes of the illumination of the partition 7, according to the distribution of the light energy, desired in the impact zone of the light ray 12.
Lorsque la chambre 3 au moins est remplie avec un liquide, préférentiellement pendant les étapes (c) et (d), on contrôle le déplacement du liquide présent dans la chambre 3, par exemple par capillarité et/ou aspiration, en sorte que la cavité 9, de l'autre côté de la cavité 8 d'incidence par rapport à la cloison perforable 7, demeure pleine ou vide de tout liquide. Une cavité 9 demeurant vide de tout liquide au moment de l'illumination avec le faisceau lumineux 5 permet en particulier de préserver le liquide ou les liquides circulant dans la carte d'analyse, de tout échauffement intempestif ou excessif.When the chamber 3 at least is filled with a liquid, preferably during steps (c) and (d), the movement of the liquid present in the chamber 3 is controlled, for example by capillarity and / or suction, so that the cavity 9, on the other side of the cavity 8 incidence relative to the perforable partition 7, remains full or empty of any liquid. A cavity 9 remaining empty of any liquid at the time of illumination with the light beam 5 makes it possible in particular to preserve the liquid or the liquids circulating in the analysis card, from any untimely or excessive heating.
Le mode de réalisation représenté aux Figures 3 à 5 diffère de celui représenté aux Figures 1 à 2, par le fait que la carte d'analyse 1 comprend "n", en l'occurrence 3 chambres 2, 3, 17, disposées en série, mais pouvant être aussi disposées en parallèle, séparées deux à deux par "n-1", en l'occurrence deux cloisons perforables 7 et 18, "n" étant un nombre entier, et l'une des chambres 2,3 et 17 pouvant être notamment une chambre de mesure optique. De manière à permettre un processus automatique avec un seul et même rayon lumineux laser, les cloisons perforables 7 et 18 sont distribuées dans la carte 1, de manière à ce que deux cloisons ne puissent pas être alignées avec un seul et même rayon lumineux incident 12. Comme indiqué précédemment, la carte d'analyse des Figures 3 à 5 comporte un liquide remplissant la chambre 3, lequel va cheminer vers la chambre 2, après perforation de la cloison 7, comme représenté à la Figure 4, puis vers la chambre 17, après perforation de la cloison 18, comme partiellement représenté à la Figure 5.The embodiment shown in Figures 3 to 5 differs from that shown in Figures 1 to 2, in that the analysis card 1 comprises "n", in this case 3 chambers 2, 3, 17, arranged in series , but can also be arranged in parallel, separated two by two by "n-1", in this case two perforable partitions 7 and 18, "n" being an integer, and one of the chambers 2,3 and 17 possibly including an optical measurement chamber. In order to allow an automatic process with a single laser light beam, the perforable partitions 7 and 18 are distributed in the card 1, so that two partitions cannot be aligned with a single incident light beam 12 As previously indicated, the analysis card of Figures 3 to 5 includes a liquid filling the chamber 3, which will flow towards the chamber 2, after perforation of the partition 7, as shown in Figure 4, then towards the chamber 17 , after perforation of the partition 18, as partially shown in Figure 5.
Comme représenté à la Figure 6, un dispositif 19 de traitement d'une carte d'analyse 1 selon l'invention comprend : - un générateur 20 de lumière cohérente, ayant la longueur d'onde prédéterminée λ et la puissance lumineuse P, comprenant une source laser, et des moyens 25 de collimâtion et de mise en forme du faisceau lumineux émis par la source laser ; un faisceau de puissance limitée, par exemple de 2 mW, est émis par un pointeur en lumière visible et superposé au faisceau laser pour son guidage ;As shown in FIG. 6, a device 19 for processing an analysis card 1 according to the invention comprises: - a generator 20 of coherent light, having the predetermined wavelength λ and the light power P, comprising a laser source, and means 25 for collimating and shaping the light beam emitted by the laser source; a beam of limited power, for example 2 mW, is emitted by a pointer in visible light and superimposed on the laser beam for its guidance;
- des moyens optiques 29 de couplage du faisceau lumineux quasi-parallèle, pour injecter ce dernier dans une fibre optique 21, ou des moyens optiques de guidage tels que prisme ou lentille, avec un bon rendement ;- Optical means 29 for coupling the quasi-parallel light beam, to inject the latter into an optical fiber 21, or optical guide means such as a prism or lens, with good efficiency;
- des moyens optiques 30 de collimation et/ou de mise en forme du faisceau, permettant d'obtenir un "spot" circulaire de diamètre et de densité de puissance, déterminés sur le plan de travail où le perçage sera effectué, situé sur ou dans chaque cloison 7 ou 18 ;- optical means 30 for collimating and / or shaping the beam, making it possible to obtain a circular "spot" of diameter and power density, determined on the work surface where the drilling will be carried out, located on or in each partition 7 or 18;
- des moyens 51 de positionnement de la carte d'analyse 1, dans un plan perpendiculaire par rapport à la direction de référence 6, en sorte que l'incidence du rayon lumineux 12 illuminant chaque cloison perforable 7 ou 18, soit sensiblement perpendiculaire à chaque dite cloison ; ces moyens de positionnement 51 assurent un déplacement de la carte d'analyse selon deux directions de référence 22 et 23 sensiblement perpendiculaires. A titre d'exemple, le générateur 20 émet une lumière à 800 nm, avec une lumière de guidage à 670 nm. La puissance de la source lumineuse ainsi constituée est demeans 51 for positioning the analysis card 1 in a plane perpendicular to the reference direction 6, so that the incidence of the light ray 12 illuminating each perforable partition 7 or 18 is substantially perpendicular to each said partition; these positioning means 51 ensure a displacement of the analysis card in two reference directions 22 and 23 substantially perpendicular. For example, the generator 20 emits light at 800 nm, with guide light at 670 nm. The power of the light source thus constituted is
700 mW, pour une puissance réellement utilisée de l'ordre de 300 mW. La fibre optique 21 utilisée est une fibre multimode de diamètre de coeur de 200 μm. A la sortie de la fibre optique 21, une lentille 30 permet de focaliser l'énergie lumineuse sur le centre 13, situé sur ou dans chaque cloison 7 ou 18.700 mW, for a power actually used of the order of 300 mW. The optical fiber 21 used is a multimode fiber with a core diameter of 200 μm. At the exit of the optical fiber 21, a lens 30 makes it possible to focus the light energy on the center 13, located on or in each partition 7 or 18.
Avec les conditions suivantes : - cloison 7 ou 18 réalisée en polystyrène, ayant une épaisseur de 0,3 mm ;With the following conditions: - partition 7 or 18 made of polystyrene, having a thickness of 0.3 mm;
- parois externes 15 et 16, réalisées en polypropylene, silicium ou germanium, selon une épaisseur de 2 à 7/10è de mm pour un film en polypropylene ; - un générateur 20 de lumière cohérente, tel que décrit précédemment ; - aucun liquide n'étant présent dans les chambres 2 et 3 et cavités 8 et 9 ; il a été possible de percer un trou 7a, de diamètre 0,5 mm, en deux secondes environ.- external walls 15 and 16, made of polypropylene, silicon or germanium, with a thickness of 2 to 7 / 10ths of a mm for a polypropylene film; - a generator 20 of coherent light, as described previously; - no liquid being present in chambers 2 and 3 and cavities 8 and 9; it was possible to drill a hole 7a, with a diameter of 0.5 mm, in about two seconds.
La présente invention peut être mise en oeuvre selon différents modes d'exécution ; en particulier l'une des chambres, mise en communication selon la présente invention, peut elle-même communiquer avec l'atmosphère ou le milieu dans lequel se trouve la carte d'analyse. The present invention can be implemented according to different embodiments; in particular one of the chambers, placed in communication according to the present invention, can itself communicate with the atmosphere or the environment in which the analysis card is located.

Claims

REVENDICATIONS
1/ Carte d'analyse (1), dans laquelle sont ménagées deux chambres (2,3) isolées l'une par rapport à l'autre, caractérisée en ce que les deux chambres sont séparées l'une de l'autre par une cloison (7), perforable, disposée au sein de ladite carte, en matériau absorbant, notamment matière plastique, absorbant une énergie lumineuse, ayant au moins une longueur d'onde prédéterminée λ, pour la transformer en une énergie thermique susceptible d'éliminer au moins localement ledit matériau, deux cavités (8,9) sont ménagées de part et d'autre de la cloison (7), et sont en communication avec ou comprises dans respectivement les deux chambres (2,3) respectivement, une fenêtre (10,11) en un matériau transparent au moins pour la longueur d'onde λ est disposée en vis-à-vis de ladite cloison (7), et délimite avec cette dernière l'une (8) desdites cavités, dite d'incidence d'un rayon lumineux (12).1 / Analysis card (1), in which two chambers (2,3) are provided, insulated from one another, characterized in that the two chambers are separated from one another by a partition (7), perforable, disposed within said card, of absorbent material, in particular plastic material, absorbing light energy, having at least a predetermined wavelength λ, to transform it into thermal energy capable of eliminating at less locally said material, two cavities (8,9) are formed on either side of the partition (7), and are in communication with or included in the two chambers (2,3) respectively, a window (10 , 11) made of a transparent material at least for the wavelength λ is arranged opposite said partition (7), and delimits with the latter one (8) of said cavities, called incidence d 'a light ray (12).
2/ Carte selon la revendication 1, caractérisée en ce qu'une autre fenêtre (11) en un matériau transparent au moins pour la longueur d'onde λ, en vis-à-vis de ladite cloison (7), délimite avec cette dernière l'autre (9) desdites cavités, dite d'émergence d'un rayon lumineux.2 / card according to claim 1, characterized in that another window (11) made of a transparent material at least for the wavelength λ, facing said partition (7), delimits with the latter the other (9) of said cavities, called the emergence of a light ray.
3/ Carte selon la revendication 1, caractérisée en ce qu'elle comprend un corps (14) dans lequel sont empreintes ou gravées les deux chambres (2,3) et les deux cavités (8,9), et au moins une paroi externe (15,16) fermant lesdites chambres et cavités vis-à-vis de l'extérieur. 4/ Carte selon la revendication 3, de forme substantiellement aplatie, caractérisée en ce qu'elle comprend un corps plat (14) entre deux parois externes (15,16). 5/ Carte selon la revendication 3, caractérisée en ce que l'une (2) des chambres et la cavité (8) qui lui correspond sont fermées par l'une (15) des parois externes, et l'autre chambre (3) et l'autre cavité (9) qui lui correspond sont fermées par l'autre paroi externe (16).3 / Card according to claim 1, characterized in that it comprises a body (14) in which are imprinted or engraved the two chambers (2,3) and the two cavities (8,9), and at least one outer wall (15,16) closing said chambers and cavities vis-à-vis the outside. 4 / Card according to claim 3, of substantially flattened shape, characterized in that it comprises a flat body (14) between two external walls (15,16). 5 / Card according to claim 3, characterized in that one (2) of the chambers and the cavity (8) which corresponds to it are closed by one (15) of the external walls, and the other chamber (3) and the other cavity (9) which corresponds to it are closed by the other external wall (16).
6/ Carte selon la revendication 3, caractérisée en ce que la cloison perforable (7) est formée dans le corps plat (14), dont le matériau constitutif est absorbant de l'énergie lumineuse de longueur d'onde λ.6 / card according to claim 3, characterized in that the perforable partition (7) is formed in the flat body (14), whose constituent material is absorbing light energy of wavelength λ.
7/ Carte selon la revendication 3, caractérisée en ce que la fenêtre (10) est formée dans la paroi externe (15) , dont le matériau constitutif est transparent pour la longueur d'onde λ. 8/ Carte selon la revendication 1, caractérisée en ce qu'elle comprend n chambres (2,3,17) disposées en série et/ou parallèle, séparées deux à deux par n-1 cloisons perforables (7,18), "n" étant un nombre entier, et l'une des chambres étant notamment une chambre de mesure optique, lesdites cloisons perforables étant distribuées dans ladite carte, de manière à ce que deux cloisons ne puissent pas être alignées avec un seul et même rayon lumineux incident (12).7 / card according to claim 3, characterized in that the window (10) is formed in the outer wall (15), the constituent material is transparent for the wavelength λ. 8 / card according to claim 1, characterized in that it comprises n chambers (2,3,17) arranged in series and / or parallel, separated two by two by n-1 perforable partitions (7,18), "n "being an integer, and one of the chambers being in particular an optical measurement chamber, said perforable partitions being distributed in said card, so that two partitions cannot be aligned with a single incident light ray ( 12).
9/ Carte d'analyse prête à l'emploi, selon la revendication 1, dont au moins une chambre (3) est remplie avec un liquide. 9 / Ready-to-use analysis card according to claim 1, at least one chamber (3) of which is filled with a liquid.
EP97901736A 1996-02-12 1997-02-12 Analysis card Expired - Lifetime EP0820348B1 (en)

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FR9601984A FR2744803B1 (en) 1996-02-12 1996-02-12 METHOD AND DEVICE FOR PROCESSING AN ANALYSIS CARD
FR9601984 1996-02-12
PCT/IB1997/000112 WO1997028899A1 (en) 1996-02-12 1997-02-12 Analysis card

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CA2218415A1 (en) 1997-08-14
ATE211657T1 (en) 2002-01-15
FR2744803A1 (en) 1997-08-14
WO1997028899A1 (en) 1997-08-14
DE69709499D1 (en) 2002-02-14
CA2218415C (en) 2006-05-30
EP0820348B1 (en) 2002-01-09
US5869002A (en) 1999-02-09
DE69709499T2 (en) 2002-09-26
FR2744803B1 (en) 1998-03-13

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