EP0781415A1 - Quick test for the differentiation of the features of erythrocytes - Google Patents
Quick test for the differentiation of the features of erythrocytesInfo
- Publication number
- EP0781415A1 EP0781415A1 EP95931995A EP95931995A EP0781415A1 EP 0781415 A1 EP0781415 A1 EP 0781415A1 EP 95931995 A EP95931995 A EP 95931995A EP 95931995 A EP95931995 A EP 95931995A EP 0781415 A1 EP0781415 A1 EP 0781415A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- paper
- rapid test
- test according
- zones
- blood
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/554—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being a biological cell or cell fragment, e.g. bacteria, yeast cells
- G01N33/555—Red blood cell
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
Definitions
- the invention relates to a rapid test for differentiating erythrocyte characteristics, particularly suitable for use as a bedside test for the simple and quick determination of blood groups or their confirmation from capillary blood, but which can also be carried out with erythrocyte suspensions if the cell density corresponds to that of whole blood.
- the test card can also be used to document the test.
- Bedside tests are prescribed as confirmation tests immediately before the transfusion, since the donor is generally not present during the transfusion in order to avoid confusion of preserved blood. Such tests have been described since about 1908 and were first carried out in the presence of the donor. Since 1955, an ABO / Rh bedside test has been used on cardboard boxes with dried-in antisera, followed by tests with ready-to-use, liquid antisera, then dipsticks based on Elisa.
- the test according to the invention is based on the method of capillary analysis or paper chromatography based on Runge and Schönbein.
- the paper-chromatographic separation of proteins is a common but time-consuming method.
- the determination of ABO group antigens in decayed blood using ascending paper chromatography in hydrochloric acid / methanol Systems described the antigens are determined after subsequent elution (Kuchkinov, A textbook. Vopr. Sud. Med. Ekspert. Veshchestv. Dokazatel'stv, 1982, 38-44 (Chemical Abstracts 101, 1984, 67173)).
- This cumbersome procedure is of course completely unsuitable for a bedside test, in which speed, accuracy and documentability are important.
- the object of the invention is therefore to provide a quick, easy-to-use, safe and documentable test for blood group confirmation.
- capillary analytical blood group confirmation can be carried out using paper and a running medium, erythrocytes being transported through the running medium and permanent migration being available due to their different migration. If suitable antisera are added, this transport of the erythrocytes is blocked by their agglutination, since the agglutinates are immobile. Chromatography papers do not allow a differentiation between agglutinated and non-agglutinated bleeding, although the paper production for these papers has been optimized for consistency and reproducibility.
- the invention therefore relates to a rapid test for differentiating erythrocyte features on paper, on which the corresponding antisera are applied, and the differentiation is possible optically and permanently through the different migration of the erythrocytes by means of an eluent.
- Another object of the invention is therefore the use of the test according to the invention for the rapid determination of blood groups, their differentiation and / or the determination of blood group incompatibilities.
- Another object of the invention is a so-called
- Bedside test card for the quick and safe check of donor and recipient blood directly before the transfer and its documentation, as well as the production of these cards.
- Suitable types of paper for the test according to the invention are unsized papers, preferably filter paper (such as laboratory filter paper or coffee filters), flow paper (such as kitchen paper, blotting paper, dry wipes made of paper or absorbent paper, for example for laboratory tables) or hygienic paper (such as toilet paper or household towels, towels, handkerchiefs) and paper napkins). Papers of the quality of toilet paper, wipes, coffee filters, absorbent paper are particularly preferred, paper qualities for coffee filters and absorbent paper for laboratory tables being very particularly preferred.
- the papers can preferably also have a one-sided coating of solvent-impermeable material, preferably polyethylene, by means of which the attachment to the documentation test card is made considerably easier and which prevents solvents and test substances from penetrating into the test card.
- FIG. 1 A practical embodiment of the rapid test according to the invention is shown as an example in FIG. 1.
- the analysis field (3) has a comb-like appearance with several running zones (5), preferably four.
- the width of the running zones (5) being able to be reduced to up to 0.5 mm and the distances between the running zones (5) being reduced to 0.1 mm.
- the antisera applied in the antisera application zone (5) are mono- or polyclonal commercial preparations with the specificities anti-A, anti-B and anti-D, and monoclonal antisera are particularly preferred. They were found to be optimal for this procedure. At these positions, 2-5 ⁇ l antiserum in commercial concentration is applied manually as required and the blood group systems to be tested. For standard examinations, the antisera are preferably already applied industrially by customary printing processes.
- the capillary blood (from the fingertip or the earlobe) or the donor blood is first placed on the storage field (8), which preferably consists of an impermeable and indifferent Foil exists from which the individual test fields are fed.
- the remainder can preferably also be covered with a film after drying and serves as a reference sample for tests that may be required later, especially forensic tests.
- 2-5 ⁇ l are then added to the test blood application zone (5).
- a flow agent suitable for the transport of the erythrocytes such as, for example, LISS (lower ionic strength solution) or PBS (phosphate buffered saline) solutions, preferably physiological saline, is used as the flow agent for the analysis.
- LISS lower ionic strength solution
- PBS phosphate buffered saline
- the superplasticizer front will reach the end of the running zone (7) after a few seconds, with the blood stain on agglutination at the application zone of the antisera (6 ) remains or moves with the superplasticizer if the agglutination is weak or missing.
- the analysis field (3) shown in FIG. 1 is only to be regarded as an example within the scope of the invention and the restriction to 4 running zones (7) is only one possible embodiment. Embodiments with only one running zone and with any number are of course possible, so that a single test can be used to test for further antisera. Miniaturization is also possible, the width of the running zones (5) being able to be reduced to up to 0.5 mm and the distances between the running zones (5) being reduced to 0.1 mm. As a result, many can do so in a small space Different tests are done side by side with a very small amount of test blood.
- a particular advantage of the test according to the invention is also the considerably reduced amount of test blood. Compared to 70 to 100 ⁇ l in previous tests, only 2 to 5 ⁇ l blood are required.
- Monoclonal antisera of the specificities Anti-M and Anti-N, as well as Anti-Le-a and Anti-Le-b have also been tested in this way as rapid tests and offer the above-mentioned tests from capillary blood as well as from erythrocyte suspension. decisive time advantage in the Sehnell selection of blood supplies.
- the test is therefore generally suitable for the rapid differentiation of erythrocyte characteristics with monoclonal antibodies of the IgM class, for example the Duffy, Kell-Cellano, Kidd, Lewis, Lutheran, MNSs, P , Rhesus, HLA system or the plasma protein polymorphism.
- the rapid test according to the invention is particularly suitable for the industrial production of bedside test cards, in that a card made of paper, cardboard or a suitable plastic material is pre-printed with the necessary information and fields for receiving information. Then the analysis field (3) made of coated paper and the reference field (8) for taking the blood sample are glued on.
Abstract
Description
Claims
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19944432485 DE4432485A1 (en) | 1994-09-13 | 1994-09-13 | Rapid test for the differentiation of erythrocyte characteristics |
DE4432485 | 1994-09-13 | ||
PCT/EP1995/003493 WO1996008723A1 (en) | 1994-09-13 | 1995-09-06 | Quick test for the differentiation of the features of erythrocytes |
Publications (1)
Publication Number | Publication Date |
---|---|
EP0781415A1 true EP0781415A1 (en) | 1997-07-02 |
Family
ID=6528042
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP95931995A Withdrawn EP0781415A1 (en) | 1994-09-13 | 1995-09-06 | Quick test for the differentiation of the features of erythrocytes |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP0781415A1 (en) |
AU (1) | AU3522295A (en) |
DE (1) | DE4432485A1 (en) |
WO (1) | WO1996008723A1 (en) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU772847B2 (en) | 1998-11-12 | 2004-05-06 | Invitrogen Corporation | Transfection reagents |
DE10244154A1 (en) * | 2002-09-23 | 2004-04-08 | Prisma Diagnostika Gmbh | Carrier element for diagnostic tests |
EP3169310A1 (en) | 2014-07-15 | 2017-05-24 | Life Technologies Corporation | Compositions with lipid aggregates and methods for efficient delivery of molecules to cells |
CN117268877B (en) * | 2023-11-21 | 2024-02-20 | 军科正源(北京)药物研究有限责任公司 | Method for detecting nerve growth factor in human tear and method for treating tear |
-
1994
- 1994-09-13 DE DE19944432485 patent/DE4432485A1/en not_active Withdrawn
-
1995
- 1995-09-06 AU AU35222/95A patent/AU3522295A/en not_active Abandoned
- 1995-09-06 WO PCT/EP1995/003493 patent/WO1996008723A1/en not_active Application Discontinuation
- 1995-09-06 EP EP95931995A patent/EP0781415A1/en not_active Withdrawn
Non-Patent Citations (1)
Title |
---|
See references of WO9608723A1 * |
Also Published As
Publication number | Publication date |
---|---|
DE4432485A1 (en) | 1996-03-14 |
AU3522295A (en) | 1996-03-29 |
WO1996008723A1 (en) | 1996-03-21 |
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Legal Events
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PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
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17P | Request for examination filed |
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