EP0764266A1 - Siphons modifies garantissant une precision de dosage ameliore - Google Patents

Siphons modifies garantissant une precision de dosage ameliore

Info

Publication number
EP0764266A1
EP0764266A1 EP95922222A EP95922222A EP0764266A1 EP 0764266 A1 EP0764266 A1 EP 0764266A1 EP 95922222 A EP95922222 A EP 95922222A EP 95922222 A EP95922222 A EP 95922222A EP 0764266 A1 EP0764266 A1 EP 0764266A1
Authority
EP
European Patent Office
Prior art keywords
chamber
rotor
siphon
inlet
liquid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP95922222A
Other languages
German (de)
English (en)
Other versions
EP0764266A4 (fr
Inventor
Anne R. Kopf-Sill
Carol T. Schembri
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abay SA
Abaxis Inc
Original Assignee
Abay SA
Abaxis Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abay SA, Abaxis Inc filed Critical Abay SA
Publication of EP0764266A1 publication Critical patent/EP0764266A1/fr
Publication of EP0764266A4 publication Critical patent/EP0764266A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/07—Centrifugal type cuvettes

Definitions

  • the present invention relates generally to devices and methods for analyzing biological fluids.
  • it relates to the design and use of improved centrifugal rotors having siphons which allow delivery of a precise volume of liquid to a chamber in the rotor.
  • Biological tests of blood plasma and other biological fluids frequently require that fluids be quickly divided into predetermined volumes for analysis in a variety of optical tests or assays. It is also frequently desirable to separate potentially interfering cellular components of the material from the other fluid prior to testing.
  • Such measurement and separation steps have previously been typically performed by centrifugation to separate, for instance, blood plasma from the cellular components, followed by manual or automated pipetting of predetermined volumes of the blood plasma into separate test wells.
  • centrifugal rotors are designed to measure volumes of a biological fluid, such as blood, remove cellular components, and mix the fluid with an appropriate diluent for analysis, for example by optical testing.
  • the rotors provide a plurality of discrete volumes of sample in separate cuvettes in which the sample is optically analyzed. To ensure accurate and consistent results, such rotors require the delivery of precisely measured volumes of liquid to various chambers in the rotor.
  • U.S. Patent Nos. 4,894,204, and 5,160,702 disclose siphons for transferring fluids between chambers in a rotor.
  • U.S. Patent No. 4,244,916 discloses a rotor comprising a plurality of cuvettes positioned radially outward of a central receptacle. Each cuvette is connected to the central receptacle by a duct and comprises a separate air escape orifice.
  • U.S. Patent No. 4,902,479 discloses a multi- cuvette rotor comprising elongated, radially extending cuvettes. Each elongated cuvette comprises a first chamber for receiving a first constituent and a second chamber for receiving a second constituent. A divider structure between the first and second chambers prevents mixing of the constituents before a predetermined time. Mixing occurs as the rotor is spun at a sufficient speed.
  • U.S. Patent No. 5,077,013 discloses rotors comprising peripheral cuvettes connected to holding chambers positioned radially inward from the cuvettes.
  • the present invention provides centrifugal rotors comprising siphons for delivering a premeasured volume of liquid, typically a biological sample such as plasma, between a first and a second chamber in the rotor.
  • the siphons of the invention have an elbow that is radially inward of the radially most inward point of the fluid in the first chamber. As the rotor is spinning the fluid does not flow past the elbow. After the rotor stops, capillary forces "prime" the siphon by pulling fluid just around the elbow.
  • the siphons of the invention are designed such that the inlet of the siphon on the first chamber is radially outward of the siphon outlet on the second chamber.
  • the positioning of the inlets and outlets of the siphons of the invention provide a number of advantages.
  • the inlet of the siphon is always positioned radially outward of the final position of the meniscus of the fluid in the first chamber, after fluid has been transferred to the second chamber.
  • inaccuracy in measurement associated with different shaped menisci in different fluids is minimized since the meniscus is minimized.
  • all siphons are semi-stable because the train of fluid in a siphon is stable but easily broken if the rotor is perturbed. When the train of fluid is broken, under centrifugal force, the fluid contained in the siphon will flow to the radially most outward point. In prior art siphons this point is the siphon outlet.
  • the radially most outward point in the siphon is the siphon inlet.
  • the problem of delivering unmetered volumes of fluid is avoided because the fluid flows back into the first chamber when the train of fluid is broken.
  • the chambers connected by the siphons of the invention are used to perform any of a number of functions, such as metering liquids, separating solid components from a sample, mixing diluent with the sample, and the like.
  • the siphons connect a plasma metering chamber to a mixing chamber for mixing the premeasured volume of plasma with diluent.
  • the rotors of the invention comprise unmodified inlet channels connecting a distribution ring to cuvettes comprising reagents for optical analysis of a biological sample.
  • the inlet channels are sized such that, as the rotor spins, gas escapes from the cuvette through the inlet channel as the liquid enters the cuvette through the inlet channel.
  • an "unmodified inlet channel” as used herein refers to a simple inlet channel, typically having a rectangular cross section, which is not modified (e.g., by altering the cross-sectional shape, surface texture, and the like) to provide a pathway for gas to escape from a cuvette that is not otherwise vented.
  • FIGs. 1A-1F are top plan views of a rotor of the invention showing the flow of fluids through the chambers and channels of the rotor as the rotor is spun.
  • the present invention provides methods and devices for the delivery of liquids to chambers in an analytical rotor.
  • the rotors of the invention comprise siphons which ensure precise delivery of etered volumes of liquid to a desired chamber in the rotor.
  • the rotors of the invention are suitable for the analysis of any liquid, typically a biological sample such as whole blood or plasma. It is also useful with numerous other biological fluids, such as urine, sputum, semen, saliva, ocular lens fluid, cerebral fluid, spinal fluid, amniotic fluid. Other fluids that can be tested include tissue culture media, food and industrial chemicals, environmental samples and the like.
  • the rotors typically provide chambers which can separate cellular components from the biological sample (e.g. whole blood) , measure a precise volume of liquid sample (e.g. plasma) , mix the sample with an appropriate diluent and deliver the diluted sample to cuvettes for optical analysis.
  • the fluid delivered to the cuvettes undergoes reaction(s) within the cuvettes, e.g., reaction with a reagent which forms part of an analytical procedure to detect one or more analytes within the fluid.
  • the sample may further be optically analyzed while present in the rotor, either with or without prior reaction.
  • the apparatus of the present invention comprises an analytical rotor having a rotor body which is capable of being mounted on a conventional laboratory centrifuge of the type which is commercially available from suppliers, such a Beckman Instruments, Inc., Spinco Division, Fullerton, California; Fisher Scientific, Pittsburgh, Pennsylvania; VWR Scientific, San Francisco, California, and the like.
  • the centrifugal rotor will include a receptacle or other coupling device suitable for mounting on a vertical drive shaft provided by the centrifuge.
  • the particular design of the receptacle or coupling device will depend on the nature of the centrifuge, and it will be appreciated that the centrifugal rotor of the present invention may be adapted for use with all or most types of centrifuges which are now available or which may become available in the future.
  • the rotor body comprises a structure which maintains a desired geometric pattern or relationship between a plurality of chambers, interconnecting passages, and vents, as described in more detail below.
  • Various specialized chambers and channels suitable for use in the rotors of the invention are disclosed in U.S. Patent Nos. 5,061,381 and 5,122,284, and U.S.S.N. 07/678,762 and 07/783,041 which are incorporated herein by reference.
  • the body will be a substantially solid plate or disk with the chambers and passages formed as spaces or voids in the otherwise solid matrix.
  • such solid plate structures may be formed by laminating a plurality of separately-formed layers together into a composite structure where the chambers and horizontal passages are generally formed between adjacent layers.
  • the vertical passages may be formed through the layers.
  • the individual layers may be formed by injection molding, machining, or combinations thereof, and will usually be joined together, typically using a suitable adhesive or by ultrasonic welding. The final enclosed volumes are formed when the layers are brought together.
  • centrifugal rotor could also be formed as a plurality of discrete components, such as tubes, vessels, chambers, etc., arranged in a suitable framework.
  • the rotor body may be formed from a wide variety of materials and may optionally include two or more materials. Usually, the material(s) will be transparent so that the presence and distribution of the biological fluid, cellular components, and reagents, may be observed within the various internal chambers and passages.
  • analytical chambers e.g., cuvettes, or other test wells are formed within the rotor
  • suitable optical paths formed within the rotor so that the contents of the cuvettes may be observed spectrophotometrically, fluorometrically, or by other optical assessment instruments.
  • suitable cuvettes having particular optical paths formed therethrough is disclosed in U.S. Patent No. 5,173,193, the disclosure of which is incorporated herein by reference.
  • the rotor is formed with an acrylic resin having suitable optical properties, at least in those areas which define an optical path.
  • the apparatus and method of the present invention are suitable for performing a wide variety of analytic procedures and assays which are beneficially or necessarily performed on blood plasma and other samples.
  • the analytic procedures may require that the sample be combined with one or more reagents so that some detectable change occurs which may be related to the presence and/or amount of a particular component (analyte) or characteristic of the sample.
  • the sample may undergo a reaction or other change which results in a change in color, fluorescence, luminescence, or the like, which may be measured by conventional spectrophotometers, fluorometers, light detectors, and the like.
  • immunoassays and other specific binding assays may be performed within the cell-free fluid collection chamber or within cuvettes which are connected to the collection chamber.
  • such assay procedures should be homogeneous and not require a separation step.
  • it may be possible to accommodate heterogeneous assay systems by providing a means to separate the sample (e.g., blood plasma) from the collection chamber or another test well or cuvette after the immunological reaction step has occurred.
  • the means of analyzing the sample is not an important aspect of the invention. Any of a number of analytical methods can be adapted for use in the rotors of the invention, depending upon the particular sample being analyzed and component being detected.
  • assays In the case of blood analyses, conventional blood assays are typically preformed. Examples of assays which may be performed include those designed to detect glucose, lactate dehydrogenase, serum glutamic-oxaloacetic transaminase (SGOT) , serum glutamic-pyruvic transaminase (SGPT) , blood urea nitrogen (BUN) , total protein, alkalinity, phosphatase, bilirubin, calcium, chloride, sodium, potassium, magnesium, and the like. This list is not exhaustive and is intended merely as being exemplary of the assays which may be performed using the apparatus and method of the present invention.
  • these tests will require that the blood and plasma be combined with one or more reagents which result in an optically detectable, usually photometrically detectable, change in the plasma.
  • the reagents which are required are well known and amply described in the patent and scientific literature.
  • the reagents are preferably provided in lyophilized form to increase stability. Ideally, they are provided in the form of lyophilized reagent spheres as described in U.S.S.N. 07/747,179, which is incorporated herein by reference.
  • Figure 1A shows the position of a blood sample 102 in the blood application chamber 104 after the sample has been loaded in the rotor body 100.
  • a diluent container in chamber 106 is opened upon mounting of the rotor on the spindle of the centrifuge as described in copending and commonly assigned application, U.S. Serial No. 07/873,327, which is incorporated herein by reference.
  • Fig. IB shows the position of the diluent 108 and blood sample 102 after the rotor is spun at 4,000 rpm.
  • the blood sample 102 begins to exit the blood application chamber 104 and enters the plasma metering chamber 110.
  • diluent 108 empties from the diluent container into the holding chamber 112.
  • the diluent immediately begins to enter the diluent metering chamber 114 through channel 116.
  • Fig. 1C shows the position of the liquids as the rotor 100 continues to spin.
  • the blood sample 102 has emptied the blood application chamber 104 and overflows the plasma metering chamber 110 into the overflow chamber 118 where it flows to the hemoglobin cuvette 120 and the excess blood dump 122.
  • diluent 108 fills the diluent metering chamber 114 and excess flows through channel 124 to diluent-only cuvettes 126 and excess diluent dump 127.
  • Fig. ID shows the position of the liquids at the conclusion of the first spin.
  • the blood sample 102 has separated into cells 128 and plasma 130.
  • the diluent-only cuvettes 126 are filled and a predetermined amount of diluent remains in the diluent metering chamber 114.
  • the rotor 100 is then stopped and the siphon 132 from the diluent metering chamber 114, as well as the siphon 134 from the plasma metering chamber 110, are allowed to prime, as described above.
  • Siphon 134 is a siphon of the present invention. It is connected to the plasma metering chamber 110 at inlet 138.
  • the inlet 138 is position radially outward of the siphon outlet 139, through which the siphon 134 empties into the mixing chamber 136.
  • Fig. IE shows the position of the liquids during the second spin of the rotor.
  • the diluent metering chamber 114 empties into the mixing chamber 136 through siphon 132.
  • a predetermined amount of plasma 130 is metered into the mixing chamber 136 and the two fluids are mixed, thereby forming diluted plasma 131.
  • the amount of plasma 130 delivered to the mixing chamber 136 is determined by the position of the outlet 139 on the siphon 134.
  • the final level of the plasma 133 in the plasma metering chamber 110 is at the same radial position as the outlet 139.
  • the volume of plasma delievered to the mixing chamber 136 is determined by the volume of the plasma metering chamber 110 between the exit to the overflow chamber 129 and the final level of plasma 133.
  • Fig. IF shows the position of the diluted plasma 131 as the rotor is spun during the third spin. This figure illustrates the movement of the diluted plasma 131 through the distribution ring 142 and inlet channels 144 to the cuvettes 146 and excess plasma dump 147.
  • the resistance to flow in the output siphon 140 is selected to be higher than the resistance to flow in the distribution ring 142 and the inlet channels 144 so that air present in the cuvettes 146 can escape as the cuvettes are filled.
  • siphon 140 is dimensioned such that the ratio of the cross sectional area of the inlet channels 144 to the cross sectional area of the liquid in them is greater than 2:1, preferably greater than about 4:1.
  • the cross sectional area of the inlet channels 144 is typically the same as or slightly smaller than that of the distribution channel 142 so that gas in the unvented cuvettes escapes through the inlet channels 144 and distribution 142. If the sample is plasma or diluted plasma and the channels are rectangular in cross-section, their dimensions are typically as follows: siphon: 0.150 mm depth, 0.200 mm width; distribution channel 0.300 mm depth, 0.500mm width; inlet channels: 0.150 depth, 0.500 width.

Landscapes

  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Centrifugal Separators (AREA)
  • Optical Measuring Cells (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

La présente invention concerne des rotors à force centrifuge servant à amener un volume prémesuré de liquide dans une chambre de rotor. En particulier, lesdits rotors comprennent des siphons (134) servant à amener un volume prémesuré de liquide entre une première et une seconde chambre (136) du rotor. Les siphons (134) selon l'invention sont conçus de sorte que l'entrée (138) du siphon de la première chambre se trouve radialement à l'extérieur de la sortie (139) du siphon de la seconde chambre (136). La première chambre est vidée jusqu'à un niveau correspondant à la position radiale de la sortie (139) du siphon.
EP95922222A 1994-06-06 1995-06-05 Siphons modifies garantissant une precision de dosage ameliore Withdrawn EP0764266A4 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US25440694A 1994-06-06 1994-06-06
US254406 1994-06-06
PCT/US1995/007145 WO1995033986A1 (fr) 1994-06-06 1995-06-05 Siphons modifies garantissant une precision de dosage ameliore

Publications (2)

Publication Number Publication Date
EP0764266A1 true EP0764266A1 (fr) 1997-03-26
EP0764266A4 EP0764266A4 (fr) 1998-08-05

Family

ID=22964194

Family Applications (1)

Application Number Title Priority Date Filing Date
EP95922222A Withdrawn EP0764266A4 (fr) 1994-06-06 1995-06-05 Siphons modifies garantissant une precision de dosage ameliore

Country Status (4)

Country Link
EP (1) EP0764266A4 (fr)
JP (3) JPH10501340A (fr)
CA (1) CA2192196C (fr)
WO (1) WO1995033986A1 (fr)

Families Citing this family (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5627041A (en) * 1994-09-02 1997-05-06 Biometric Imaging, Inc. Disposable cartridge for an assay of a biological sample
US6143247A (en) * 1996-12-20 2000-11-07 Gamera Bioscience Inc. Affinity binding-based system for detecting particulates in a fluid
US6709869B2 (en) 1995-12-18 2004-03-23 Tecan Trading Ag Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system
US6143248A (en) * 1996-08-12 2000-11-07 Gamera Bioscience Corp. Capillary microvalve
AU7591998A (en) 1997-05-23 1998-12-11 Gamera Bioscience Corporation Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system
US6632399B1 (en) 1998-05-22 2003-10-14 Tecan Trading Ag Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system for performing biological fluid assays
JP3969091B2 (ja) 1999-06-23 2007-08-29 株式会社日立製作所 遠心分離機及びこれを用いる試料調製装置
ATE364843T1 (de) 1999-08-11 2007-07-15 Asahi Chemical Ind Analysenkassette und flüssigkeitsförderkontroller
AU2002219979A1 (en) * 2000-12-01 2002-06-11 Burstein Technologies, Inc. Apparatus and methods for separating components of particulate suspension
US7054258B2 (en) 2000-12-08 2006-05-30 Nagaoka & Co., Ltd. Optical disc assemblies for performing assays
US7079468B2 (en) 2000-12-08 2006-07-18 Burstein Technologies, Inc. Optical discs for measuring analytes
US7091034B2 (en) 2000-12-15 2006-08-15 Burstein Technologies, Inc. Detection system for disk-based laboratory and improved optical bio-disc including same
WO2002075312A1 (fr) 2001-03-19 2002-09-26 Gyros Ab Caracterisation de variables de reaction
US7429354B2 (en) 2001-03-19 2008-09-30 Gyros Patent Ab Structural units that define fluidic functions
US7033747B2 (en) 2001-04-11 2006-04-25 Nagaoka & Co., Ltd Multi-parameter assays including analysis discs and methods relating thereto
EP2269736B1 (fr) 2001-08-28 2013-04-24 Gyros Patent Ab Microcavite microfluidique de retention microfluidique et autres structures microfluidiques
US6919058B2 (en) 2001-08-28 2005-07-19 Gyros Ab Retaining microfluidic microcavity and other microfluidic structures
US7189368B2 (en) 2001-09-17 2007-03-13 Gyros Patent Ab Functional unit enabling controlled flow in a microfluidic device
ATE457199T1 (de) * 2001-12-14 2010-02-15 Arkray Inc Probenmessvorrichtung
JP3952019B2 (ja) * 2001-12-28 2007-08-01 株式会社日立ハイテクノロジーズ 抽出装置および化学分析装置並びに化学分析方法
WO2003096008A1 (fr) * 2002-05-08 2003-11-20 Hitachi High-Technologies Corporation Analyseur chimique et appareil de diagnostic genique
US7384602B2 (en) 2002-05-08 2008-06-10 Hitachi High-Technologies Corporation Chemical analysis apparatus and genetic diagnostic apparatus
JP4210783B2 (ja) 2002-09-26 2009-01-21 アークレイ株式会社 分析用具
JP4262466B2 (ja) 2002-10-28 2009-05-13 アークレイ株式会社 分析用具および分析装置
JP4480170B2 (ja) * 2003-02-19 2010-06-16 独立行政法人科学技術振興機構 血液分析装置及び血液分析方法
US7390464B2 (en) 2003-06-19 2008-06-24 Burstein Technologies, Inc. Fluidic circuits for sample preparation including bio-discs and methods relating thereto
JP4619224B2 (ja) * 2005-07-27 2011-01-26 パナソニック株式会社 回転分析デバイス
DE102005048233A1 (de) * 2005-10-07 2007-04-12 Albert-Ludwigs-Universität Freiburg Vorrichtung und Verfahren zum Handhaben einer flüssigen Probe unter Verwendung einer Siphon-Struktur
DE102005048260A1 (de) * 2005-10-07 2007-04-12 Albert-Ludwigs-Universität Freiburg Verfahren und Vorrichtung zum Handhaben einer flüssigen Probe unter Verwendung einer Rotation mit einem zeitlich veränderlichen Drehvektor
JP4531677B2 (ja) * 2005-10-24 2010-08-25 パナソニック株式会社 アッセイ方法、それを実施するためのセンサデバイス、およびセンサデバイス用測定装置
WO2007116909A1 (fr) * 2006-04-04 2007-10-18 Panasonic Corporation Panneau permettant d'analyser un échantillon liquide
EP2126119A4 (fr) * 2007-03-02 2014-07-16 Corbett Res Pty Ltd Appareil et procédé d'amplification d'acide nucléique
KR100858091B1 (ko) * 2007-04-24 2008-09-10 삼성전자주식회사 시료 분배 구조를 갖는 원심력 기반의 미세유동장치 및이를 포함하는 미세유동시스템
EP2072131B1 (fr) 2007-12-13 2015-04-22 Roche Diagnostics GmbH Elément microfluide destiné au mélange d'un liquide dans un réactif
WO2009111461A2 (fr) * 2008-03-04 2009-09-11 University Of Utah Research Foundation Cellule d'écoulement microfluidique
WO2010007733A1 (fr) 2008-07-17 2010-01-21 パナソニック株式会社 Dispositif d'analyse, et procédé d'analyse utilisant le dispositif d'analyse
KR101099495B1 (ko) * 2008-10-14 2011-12-28 삼성전자주식회사 원심력 기반의 미세유동장치, 이의 제조 방법 및 이를 이용한 시료분석방법
GB2464721C (en) 2008-10-23 2013-08-14 Biosurfit Sa Jet deflection device
GB2466644B (en) * 2008-12-30 2011-05-11 Biosurfit Sa Liquid handling
JP5174723B2 (ja) 2009-03-12 2013-04-03 パナソニック株式会社 分析用デバイス
GB2476474B (en) 2009-12-22 2012-03-28 Biosurfit Sa Surface plasmon resonance detection system
GB2479139A (en) 2010-03-29 2011-10-05 Biosurfit Sa A liquid distribution and metering device
EP2486978A1 (fr) 2010-10-28 2012-08-15 Roche Diagnostics GmbH Support de test microfluidique destiné à répartir une quantité de liquide en quantités partielles
EP2455162A1 (fr) 2010-10-29 2012-05-23 Roche Diagnostics GmbH Elément micro-fluidique destiné à l'analyse d'un échantillon de liquide
US9562262B2 (en) 2011-03-08 2017-02-07 UNIVERSITé LAVAL Fluidic centripetal device
AU2012255142B2 (en) 2011-05-18 2015-03-05 Diasorin Italia S.P.A. Systems and methods for valving on a sample processing device
KR20140022399A (ko) 2011-05-18 2014-02-24 쓰리엠 이노베이티브 프로퍼티즈 컴파니 샘플 처리 장치 상의 체적 계량을 위한 시스템 및 방법
ES2870874T3 (es) 2011-05-18 2021-10-27 Diasorin S P A Sistemas y métodos para detectar la presencia de un volumen seleccionado de material en un dispositivo de procesamiento de muestra
JP6257521B2 (ja) 2011-12-08 2018-01-10 バイオサーフィット、 ソシエダッド アノニマ 逐次分注および沈降速度の指標の決定
CN103890591B (zh) 2012-07-24 2016-08-24 松下健康医疗控股株式会社 分析用仪器
ES2686359T3 (es) 2013-04-15 2018-10-17 Becton, Dickinson And Company Dispositivo de recogida de fluidos biológicos
USD799715S1 (en) 2015-10-23 2017-10-10 Gene POC, Inc. Fluidic centripetal device
EP3474993B1 (fr) 2016-06-27 2024-06-12 Zoetis Services LLC Dispositifs à conduits modifiés
WO2018165440A1 (fr) * 2017-03-08 2018-09-13 Northwestern University Dispositifs, systèmes, et procédés de préparation et d'analyse d'échantillons faisant appel à des forces capillaires et centrifuges
CN112169853B (zh) * 2020-12-01 2021-02-26 南京岚煜生物科技有限公司 一种多功能微流控检测芯片

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995006870A1 (fr) 1993-09-01 1995-03-09 Abaxis, Inc. Canaux d'entree simplifies

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2572533B1 (fr) * 1984-10-26 1986-12-26 Guigan Jean Procede destine a realiser l'analyse medicale d'un echantillon liquide a l'aide d'au moins un reactif liquide et dispositif pour la mise en oeuvre du procede
FR2575293B1 (fr) * 1984-12-21 1987-03-20 Inovelf Sa Rotor a pipetage dynamique pour dispositif d'analyse a centrifugation
FR2579756B1 (fr) * 1985-03-26 1987-05-07 Guigan Jean Procede pour realiser des analyses biologiques utilisant des reactions immunologiques et dispositif de mise en oeuvre
US4999304A (en) * 1987-12-28 1991-03-12 Miles Inc. Dynamic braking centrifuge
US5160702A (en) * 1989-01-17 1992-11-03 Molecular Devices Corporation Analyzer with improved rotor structure
US5256376A (en) * 1991-09-12 1993-10-26 Medical Laboratory Automation, Inc. Agglutination detection apparatus
US5304348A (en) * 1992-02-11 1994-04-19 Abaxis, Inc. Reagent container for analytical rotor
AU4047493A (en) * 1992-04-02 1993-11-08 Abaxis, Inc. Analytical rotor with dye mixing chamber

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995006870A1 (fr) 1993-09-01 1995-03-09 Abaxis, Inc. Canaux d'entree simplifies
US5591643A (en) 1993-09-01 1997-01-07 Abaxis, Inc. Simplified inlet channels

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO9533986A1

Also Published As

Publication number Publication date
WO1995033986A1 (fr) 1995-12-14
EP0764266A4 (fr) 1998-08-05
JP2006317467A (ja) 2006-11-24
JP4178169B2 (ja) 2008-11-12
JP2008157960A (ja) 2008-07-10
JPH10501340A (ja) 1998-02-03
CA2192196C (fr) 2004-11-23
CA2192196A1 (fr) 1995-12-14

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