Classifications

• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K38/00—Medicinal preparations containing peptides
  • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
  • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
  • A61P1/02—Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
  • A61P31/04—Antibacterial agents
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
  • A61P31/10—Antimycotics

Definitions

• antiplaque agents should be designed to control rather than eliminate the dental plaque microflora (P.D. Marsh, Brit. Dent. J. 9, 174-77 (1991) .
• the oral cavity possesses a natural microflora which is bene ⁇ ficial to the host. Changes in the natural microflora may result in caries and periodontal disease.
• the invention concerns the use of these krill enzymes (KE) to prevent dental plaque on natural and artificial teeth, dental appliances, implants and soft tissues of the mouth.
• KE krill enzymes
• compositions of enzymes from Antarctic krill have been studied as potential oral prophylactic antiplaque/agents.
• a number of in vitro/in vivo studies have demonstrated that krill enzymes:
• krill enzymes offer highly promising properties as an antiplaque agent due to the high efficiency of krill enzymes in dissolving plaques and apparent biocompa- tibility.
• Oral diseases such a ⁇ caries, gingivitis/periodontitis and candidiasis are caused when microorganisms of the natural oral microflora accumulate on retentive surfaces such a ⁇ teeth and artifical re ⁇ torations, thereby forming dental plaque (1,2,3). Poor or inadequate oral hygiene causes this plaque formation. Mechanical toothbrushing i ⁇ ⁇ till the mo ⁇ t efficient way to remove dental plaque (4, 15).
• a ⁇ dental plaque consist of oral bacteria/microbes which aggregate on dental/oral surfaces by adherence, growth and production of extracellular organic matrix (18) , enzymes for debriding this organic debris have for a long time been con ⁇ sidered an ideal dental plaque remover (4) .
• Peptidase, pancr- ea ⁇ e, dextrana ⁇ e ⁇ and Alcalase ® (12) are all different enzymes tried over the years as dental plaque debriders with limited clinical success, the main reason for failure being weaknes ⁇ in cataly ⁇ t action and in retaining adequate and ⁇ ufficient enzyme concentrations on substrates in the saliva- rich oral environment (4) .
• yeast-cell ⁇ Candida albican ⁇
• denture ⁇ denture ⁇
• oral ⁇ oft ti ⁇ ue cau ⁇ e inflammatory reactions which are difficult to cure.
• the main reason i ⁇ again, poor denture hygiene (5,6).
• Enzyme-containing denture-cleaner ⁇ are already on the market Alcala ⁇ e ® being the more prominent (8, 9, 10, 12).
• krill enzyme ⁇ have proved effective in prevention of dental plaque and yea ⁇ t-cell adherence affecting adherence and metaboli ⁇ m of oral microorgani ⁇ m ⁇ and further improving the reduction of underlying inflammation ⁇ in ⁇ oft ti ⁇ ues.
• proteolytic activity krill enzymes is often quantified in ca ⁇ ein units.
• the proteolytic activity is mea ⁇ ured a ⁇ a part of the total enzymatic activity, which may comprise carbohydrate-splitting and lipolytic activity
• Krill enzymes were extracted from the raw material, krill (Euphausia superba) according to standard procedure ⁇ described in (EP-A 107,634, EP-A 177,605).
• krill wa ⁇ frozen immediately after caught and ⁇ tored at -30°C before preparation.
• the thawed krill wa ⁇ mixed with water, homogeni ⁇ ed and centrifuged in the cold.
• the ⁇ upernatant was decanted and saved. Lipids were removed by extraction with organic ⁇ olvents, the water phase concentrated and freeze- dried thus resulting in a "crude krill enzyme preparation" suitable for dental use.
• a dental pa ⁇ te or gel i ⁇ prepared with the following compo ⁇ i- tion: Sodium monofluoropho ⁇ phate, dicalcium pho ⁇ phate dihydrate, giyceroi, sorbitol, sodium citrate, ⁇ odium lauryl sulphate, aromatic flavour, sodium ⁇ accharinate, ethyl- hydroxy-4-benzoate, krill enzyme extract, citric acid colour ⁇ ing agents.
• a chewing gum is prepared with the following composition: Chewing gum base, ⁇ odium fluoride, krill enzyme extract.
• a mouth wa ⁇ h and pro ⁇ the ⁇ i ⁇ cleaner i ⁇ prepared with the following compo ⁇ ition: Ba ⁇ e, carrier, ⁇ aline water ⁇ olution, krill enzyme extract.
• Toothbrush frequency is it i ⁇ related to plaque development and gingival health. J of Periodontology 44, 396-405.

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• Health & Medical Sciences (AREA)
• Life Sciences & Earth Sciences (AREA)
• General Health & Medical Sciences (AREA)
• Pharmacology & Pharmacy (AREA)
• Public Health (AREA)
• Chemical & Material Sciences (AREA)
• Medicinal Chemistry (AREA)
• Animal Behavior & Ethology (AREA)
• Veterinary Medicine (AREA)
• Chemical Kinetics & Catalysis (AREA)
• General Chemical & Material Sciences (AREA)
• Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
• Organic Chemistry (AREA)
• Bioinformatics & Cheminformatics (AREA)
• Oncology (AREA)
• Engineering & Computer Science (AREA)
• Communicable Diseases (AREA)
• Gastroenterology & Hepatology (AREA)
• Immunology (AREA)
• Proteomics, Peptides & Aminoacids (AREA)
• Epidemiology (AREA)
• Cosmetics (AREA)
• Enzymes And Modification Thereof (AREA)
• Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

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• 1994
  • 1994-06-07 AU AU72779/94A patent/AU700252B2/en not_active Ceased
  • 1994-06-07 EP EP94923112A patent/EP0759764A1/de not_active Withdrawn
  • 1994-06-07 WO PCT/SE1994/000549 patent/WO1995033470A1/en not_active Application Discontinuation
  • 1994-06-07 JP JP8500723A patent/JPH10500991A/ja not_active Ceased

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