EP0714400B1 - THERMODYNAMICALLY STABLE CRYSTAL FORM OF 4"-DEOXY-4"-(EPI)-METHYLAMINO AVERMECTIN B1a/B1b BENZOIC ACID SALT AND PROCESSES FOR ITS PREPARATION - Google Patents

THERMODYNAMICALLY STABLE CRYSTAL FORM OF 4"-DEOXY-4"-(EPI)-METHYLAMINO AVERMECTIN B1a/B1b BENZOIC ACID SALT AND PROCESSES FOR ITS PREPARATION Download PDF

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Publication number
EP0714400B1
EP0714400B1 EP94926502A EP94926502A EP0714400B1 EP 0714400 B1 EP0714400 B1 EP 0714400B1 EP 94926502 A EP94926502 A EP 94926502A EP 94926502 A EP94926502 A EP 94926502A EP 0714400 B1 EP0714400 B1 EP 0714400B1
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EP
European Patent Office
Prior art keywords
water
epi
deoxy
benzoic acid
acid salt
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EP94926502A
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German (de)
French (fr)
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EP0714400A1 (en
Inventor
Raymond Cvetovich
Richard Demchak
James A. Mccauley
Richard J. Varsalona
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Merck Sharp and Dohme LLC
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Merck and Co Inc
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/01—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing oxygen
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H17/00—Compounds containing heterocyclic radicals directly attached to hetero atoms of saccharide radicals
    • C07H17/04—Heterocyclic radicals containing only oxygen as ring hetero atoms
    • C07H17/08—Hetero rings containing eight or more ring members, e.g. erythromycins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system

Definitions

  • the benzoic acid salt of 4"-deoxy-4"-epi-methylamino avermectin B1a/B1b is known and known to be a stable salt as described in European Patent Publication EP 0465121-A1.
  • the added stability relative to the stability of the previously described hydrochloride salt provided much greater shelf life for this important agricultural insecticide.
  • This invention is concerned with the most stable of four crystalline forms of the benzoic acid salt of 4"-deoxy-4"-epi-methyl-amino avermectin B1a/B1b a known agricultural insecticide, and processes for preparing it.
  • the four crystalline forms that have been identified are designated as forms A, B, C and D, three of which are hydrated (B, C and D).
  • the most thermodynamically stable crystalline form is the hemi-hydrate B. Knowing the most stable crystalline form and devising processes for producing the product in that form is extremely important in that it provides bulk material with crystal homogeneity that is not going to transform to another crystal habit on storage.
  • the novel processes for producing crystal form B comprises crystallization of the compound from an organic solvent containing a controlled amount of water.
  • novel compound of this invention is crystalline 4"-deoxy-4"-epi-methylamino avermectin B1a/B1b, benzoic acid salt, hemihydrate in substantially pure form.
  • R is -CH 3 (B1a) or -H (B1b) and is referred to as Crystal form B, Type B or similar designation.
  • the differential scanning calorimetry (DSC) curve determined at a heating rate of 20°C/min. under nitrogen flow in an open cup is characterized by a relatively broad water loss endotherm with a peak temperature of 74°C and a major melting/decomposition endotherm with peak temperature of 155°C, extrapolated onset temperature of 150°C with an associated heat of 56 Joules/gm.
  • the x-ray powder diffraction pattern for Type B is characterized by d-spacings of 18.13, 9.08, 8.68, 5.03, 4.61, 4.53, 3.97 and 3.82 ⁇ .
  • the aqueous solubility is pH dependent. At pH 5, acetate buffer, it has a solubility of 0.32 mg/ml.
  • the novel process of this invention comprises recrystallizing the compound in any energetic state from an aqueous organic solvent.
  • the aqueous organic solvent useful in the novel process is preferably acetonitrile with 2-4% w/w of water; methyl t-butyl ether (MTBE) with 0.5 to 0.8% w/w of water; or isopropanol (IPA) with 0.1-0.3% w/w of water.
  • MTBE methyl t-butyl ether
  • IPA isopropanol
  • Compound of crystal form A, C or D is dissolved in the organic solvent at about 50-60°C treated with the requisite amount of water, seeded with Type B crystals, cooled to and aged at about 15-30°C for about 2-4 hours and further cooled to about 5°C over a period of about 1-2 hours.
  • the crystalline product is collected on a filter and dried in vacuo.
  • the compound has significant parasiticidal activity as an anthelmintic, ectoparasiticide, insecticide and acaricide, in human and animal health and in agriculture.
  • an agricultural pesticide it has activity against insect pests of stored grains such as Tribolium sp., Tenebrio sp., and of agricultural plants such as spider mites, (Tetranychus sp.), aphids, (Acyrthiosiphon sp.); against migratory orthopterans such as locusts and immature stages of insects living on plant tissue.
  • the compound is useful as a nematocide for the control of soil nematodes and plant parasites such as Meloidogyne sp. which is of importance in agriculture.
  • the compound is active against other plant pests such as the southern army worm and Mexican bean beetle larvae.
  • the compounds are applied using known techniques as sprays, dusts, emulsions and the like, to the growing or stored crops to effect protection from such agricultural pests.
  • the compound is administered at a rate of about 5-50 gms per hectare.
  • the protection of stored crops it is normally administered by spraying with a solution containing from 0.1-10 ppm. of the compound.
  • the batch was diluted with additional acetonitrile (6 L), water (270 mL) was added, and then seed crystals of form Type B (10 g) were added at 45°C. Crystal growth began immediately and rapidly at 45°C. Crystallization must initiate, and largely occur at >35°C to assure formation of Type B crystals.
  • the batch was cooled to 20°C over a 45 min period and aged for 2.5 hrs.
  • Additional acetonitrile (2L) was added, and the slurry was cooled to 5°C over 1 hr.
  • the slurry was filtered onto a 22-in. diameter Cenco-Lapp funnel lined with paper and polypropylene.
  • the mother liquor was recycled back to the crystallization vessel to rinse and transfer the remaining product.
  • the cake was washed with cold (2°C) wet acetonitrile (5 X 4L, each 1.5% (v/v) in water).
  • the cake was partially dried with a stream of nitrogen passed through the cake for 30 min, and it was then transferred to the Hull dryer.
  • the cake was dried at 30°C in vacuo (25 in.) over 3 days to a constant weight and an acetonitrile content ⁇ 0.1 wt% by GC.
  • the batch product was 4.95 Kg of Type B material.
  • Analyses B1a anhydrous MW 1008.26 FORMULA C 56 H 81 NO 15 CFA: OFF-WHITE POWDER X-RAY CRYST.

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental Sciences (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Saccharide Compounds (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)

Abstract

The most thermodynamically stable crystalline form of the benzoic acid salt of 4"-deoxy-4"-epi-methylamino avermectin B1a/B1b as the hemihydrate is obtained by crystallization from organic solvents containing a controlled amount of water.

Description

BACKGROUND OF THE INVENTION
The benzoic acid salt of 4"-deoxy-4"-epi-methylamino avermectin B1a/B1b is known and known to be a stable salt as described in European Patent Publication EP 0465121-A1. The added stability relative to the stability of the previously described hydrochloride salt provided much greater shelf life for this important agricultural insecticide.
SUMMARY OF THE INVENTION
This invention is concerned with the most stable of four crystalline forms of the benzoic acid salt of 4"-deoxy-4"-epi-methyl-amino avermectin B1a/B1b a known agricultural insecticide, and processes for preparing it.
The four crystalline forms that have been identified are designated as forms A, B, C and D, three of which are hydrated (B, C and D). The most thermodynamically stable crystalline form is the hemi-hydrate B. Knowing the most stable crystalline form and devising processes for producing the product in that form is extremely important in that it provides bulk material with crystal homogeneity that is not going to transform to another crystal habit on storage.
The novel processes for producing crystal form B comprises crystallization of the compound from an organic solvent containing a controlled amount of water.
DETAILED DESCRIPTION OF THE INVENTION
The novel compound of this invention is crystalline 4"-deoxy-4"-epi-methylamino avermectin B1a/B1b, benzoic acid salt, hemihydrate in substantially pure form.
It has structural formula:
Figure 00020001
wherein R is -CH3 (B1a) or -H (B1b) and is referred to as Crystal form B, Type B or similar designation.
The differential scanning calorimetry (DSC) curve determined at a heating rate of 20°C/min. under nitrogen flow in an open cup is characterized by a relatively broad water loss endotherm with a peak temperature of 74°C and a major melting/decomposition endotherm with peak temperature of 155°C, extrapolated onset temperature of 150°C with an associated heat of 56 Joules/gm. The x-ray powder diffraction pattern for Type B is characterized by d-spacings of 18.13, 9.08, 8.68, 5.03, 4.61, 4.53, 3.97 and 3.82Å. The aqueous solubility is pH dependent. At pH 5, acetate buffer, it has a solubility of 0.32 mg/ml.
The novel process of this invention comprises recrystallizing the compound in any energetic state from an aqueous organic solvent.
The aqueous organic solvent useful in the novel process is preferably acetonitrile with 2-4% w/w of water; methyl t-butyl ether (MTBE) with 0.5 to 0.8% w/w of water; or isopropanol (IPA) with 0.1-0.3% w/w of water.
Compound of crystal form A, C or D is dissolved in the organic solvent at about 50-60°C treated with the requisite amount of water, seeded with Type B crystals, cooled to and aged at about 15-30°C for about 2-4 hours and further cooled to about 5°C over a period of about 1-2 hours.
The crystalline product is collected on a filter and dried in vacuo.
The utility and methods of use of the benzoic acid salt described herein and the free base thereof are well known by those skilled in the art and fully described in the scientific and patent literature such as EP 0465121.
It has significant parasiticidal activity as an anthelmintic, ectoparasiticide, insecticide and acaricide, in human and animal health and in agriculture. As an agricultural pesticide it has activity against insect pests of stored grains such as Tribolium sp., Tenebrio sp., and of agricultural plants such as spider mites, (Tetranychus sp.), aphids, (Acyrthiosiphon sp.); against migratory orthopterans such as locusts and immature stages of insects living on plant tissue. The compound is useful as a nematocide for the control of soil nematodes and plant parasites such as Meloidogyne sp. which is of importance in agriculture. The compound is active against other plant pests such as the southern army worm and Mexican bean beetle larvae.
The compounds are applied using known techniques as sprays, dusts, emulsions and the like, to the growing or stored crops to effect protection from such agricultural pests. For the treatment of growing crops, the compound is administered at a rate of about 5-50 gms per hectare. For the protection of stored crops it is normally administered by spraying with a solution containing from 0.1-10 ppm. of the compound.
EXAMPLE
Preparation of Type B Product
Materials Amounts
3.59 Kg 94.2 wt%
TYPE A MATERIAL (obtained by crystallization from MTBE) 2.33 Kg 95.7 wt%
5.92 Kg (5.61 Kg by assay)
ACETONITRILE (HPLC GRADE) 40 L (KF = 0.2 mg/ml)
WATER (DI) 570 mL
Procedure
A 50 L 4-neck round bottom flask was equipped with a mechanical stirrer, N2 inlet, thermocouple and funnel. After purging the vessel with N2, acetonitrile (12 L, KF = 0.2 mg/ml) was added and warmed to 50-55°C, and Type A material was charged. Upon complete addition (30 min.), the mixture was aged an additional 10 min. in a nitrogen atmosphere to give a clear red-brown solution.
The batch was diluted with additional acetonitrile (6 L), water (270 mL) was added, and then seed crystals of form Type B (10 g) were added at 45°C. Crystal growth began immediately and rapidly at 45°C. Crystallization must initiate, and largely occur at >35°C to assure formation of Type B crystals.
The batch was cooled to 20°C over a 45 min period and aged for 2.5 hrs. The crystalline slurry thickened during this period and made stirring difficult. Additional acetonitrile (2L) was added, and the slurry was cooled to 5°C over 1 hr. After aging at 5°C for 30 min, the slurry was filtered onto a 22-in. diameter Cenco-Lapp funnel lined with paper and polypropylene. The mother liquor was recycled back to the crystallization vessel to rinse and transfer the remaining product. The cake was washed with cold (2°C) wet acetonitrile (5 X 4L, each 1.5% (v/v) in water). The cake was partially dried with a stream of nitrogen passed through the cake for 30 min, and it was then transferred to the Hull dryer.
The cake was dried at 30°C in vacuo (25 in.) over 3 days to a constant weight and an acetonitrile content <0.1 wt% by GC. The batch product was 4.95 Kg of Type B material.
Analyses B1a anhydrous
MW 1008.26
FORMULA C56H81NO15
CFA: OFF-WHITE POWDER
X-RAY CRYST. TYPE B HYDRATE
HPLC 95.9 WT%
97.8 area%
TLC SINGLE SPOT
TITRATION 100% (Perchloric acid)
UV A% 333,245 nm in methanol
KF 1.08 wt%
GC CH3CN   <0.01%
HEXANES   <0.01%
MTBE   nd, <0.01%
IPAc   nd, <0.01%

Claims (6)

  1. A crystalline compound 4"-deoxy-4"-epi-methyl-amino avermectin B1a/B1b, benzoic acid salt, hemihydrate in substantially pure form.
  2. A process for the preparation of the crystalline compound 4"-deoxy-4"-epi-methylamino avermectin B1a/B1b, benzoic acid salt, hemihydrate (Type B) in substantially pure form which comprises recrystallizing any other form or mixtures of forms thereof from an aqueous organic solvent by dissolving the starting material in the organic solvent at a temperature of 50-60°C adding about 0.1 to 4% w/w of water; cooling to about 45-35°C; seeding with Type B crystals; holding until crystallization is essentially complete; cooling to and holding at 15 to 30°C for about 2-4 hours, cooling to about 5°C over a period of 1-2 hours, collecting the solids and drying.
  3. The process of Claim 2 wherein the aqueous organic solvent is acetonitrile with 2-4% w/w of water; methyl t-butyl ether with 0.5 to 0.8% w/w of water; or isopropanol with 0.1 to 0.3% w/w of water.
  4. The process of Claim 3 wherein the aqueous solvent is acetonitrile with 2-4% w/w of water.
  5. A method for the control of agricultural insects, which comprises applying to an area infested with such agricultural insects an effective amount of the stable salt of Claim 1.
  6. A composition useful for the treatment of insect infestations of plants or plant products which comprises an inert carrier and an effective amount of the stable salt of Claim 1.
EP94926502A 1993-08-19 1994-08-15 THERMODYNAMICALLY STABLE CRYSTAL FORM OF 4"-DEOXY-4"-(EPI)-METHYLAMINO AVERMECTIN B1a/B1b BENZOIC ACID SALT AND PROCESSES FOR ITS PREPARATION Expired - Lifetime EP0714400B1 (en)

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US10918993A 1993-08-19 1993-08-19
US109189 1993-08-19
PCT/US1994/009207 WO1995005390A1 (en) 1993-08-19 1994-08-15 THERMODYNAMICALLY STABLE CRYSTAL FORM OF 4'-DEOXY-4'-EPI-METHYLAMINO AVERMECTIN B1a/B1b BENZOIC ACID SALT AND PROCESSES FOR ITS PREPARATION

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EP0714400B1 true EP0714400B1 (en) 1999-12-15

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JP (3) JP3954093B2 (en)
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CN (1) CN1041523C (en)
AT (1) ATE187735T1 (en)
AU (1) AU684828B2 (en)
BR (1) BR9407300A (en)
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ES (1) ES2139753T3 (en)
FI (2) FI110783B (en)
GR (1) GR3032164T3 (en)
HU (1) HU217769B (en)
LV (1) LV12571B (en)
NO (1) NO305658B1 (en)
NZ (1) NZ273129A (en)
PL (1) PL176300B1 (en)
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Cited By (1)

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CN106995476A (en) * 2017-05-16 2017-08-01 河北美荷药业有限公司 A kind of preparation method of emamectin benzoate B2 benzoates

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US6486195B1 (en) * 1993-08-19 2002-11-26 Merck & Co., Inc. Thermodynamically stable crystal form of 4″-deoxy-4″-epi-methylamino avermectin B1a/B1b benzoic acid salt and processes for its preparation
CR6574A (en) 2001-02-27 2004-10-28 Syngenta Participations Ag SALTS OF SUBSTITUTED FINDINGS IN POSITION 4 WITH PESTICIATED PROPERTIES
CN102884050B (en) * 2010-02-23 2014-05-28 日本化药株式会社 Stable crystal form of 2-ethyl-3, 7-dimethyl-6-(4-(trifluoromethoxy) phenoxy)quinoline-4-ylmethyl carbonate, method of manufacturing same and agricultural chemical composition containing crystals of same
CN108840893A (en) * 2018-07-01 2018-11-20 李万强 A kind of novel crystal forms of emamectin-benzoate and preparation method thereof
CN109912671B (en) * 2019-04-12 2022-04-26 宁夏泰益欣生物科技有限公司 Method for extracting abamectin B2a by using abamectin crystallization mother liquor

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106995476A (en) * 2017-05-16 2017-08-01 河北美荷药业有限公司 A kind of preparation method of emamectin benzoate B2 benzoates

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CZ45996A3 (en) 1996-05-15
ES2139753T3 (en) 2000-02-16
SK20996A3 (en) 1996-06-05
BR9407300A (en) 1996-10-08
FI111165B (en) 2003-06-13
FI960725A0 (en) 1996-02-16
SK281684B6 (en) 2001-06-11
PT714400E (en) 2000-05-31
CZ287929B6 (en) 2001-03-14
LV12571A (en) 2000-11-20
GR3032164T3 (en) 2000-04-27
JP3954093B2 (en) 2007-08-08
HU9600345D0 (en) 1996-04-29
CA2168843A1 (en) 1995-02-23
HU217769B (en) 2000-04-28
KR100370697B1 (en) 2003-06-11
NO960618L (en) 1996-02-16
PL313043A1 (en) 1996-05-27
FI20021120A (en) 2002-06-11
CN1129453A (en) 1996-08-21
JP2011207899A (en) 2011-10-20
JP2007176946A (en) 2007-07-12
FI960725A (en) 1996-02-16
CY2181B1 (en) 2002-08-23
RU2133753C1 (en) 1999-07-27
JPH09502169A (en) 1997-03-04
NO960618D0 (en) 1996-02-16
ZA946203B (en) 1995-03-31
NZ273129A (en) 1996-08-27
NO305658B1 (en) 1999-07-05
LV12571B (en) 2001-04-20
FI110783B (en) 2003-03-31
KR960703936A (en) 1996-08-31
DE69422182T2 (en) 2000-06-21
PL176300B1 (en) 1999-05-31
DE69422182D1 (en) 2000-01-20
CN1041523C (en) 1999-01-06
CA2168843C (en) 2004-10-19
HUT73552A (en) 1996-08-28
AU684828B2 (en) 1998-01-08
DK0714400T3 (en) 2000-04-10
ATE187735T1 (en) 2000-01-15
EP0714400A1 (en) 1996-06-05
WO1995005390A1 (en) 1995-02-23

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