Classifications

• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
  • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
  • G01N21/03—Cuvette constructions
  • G01N21/0303—Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment

Definitions

• the present invention essentially relates to a device for raising the level of a liquid, in a cell, well or the like.
• Cell or multiple well plates frequently intended to contain one or more substances to be analyzed are frequently used in biology, in particular in cell biology.
• - ⁇ is the molar extinction coefficient, the value of which depends on the wavelength of the light used and the nature of the dissolved substance.
• C is the concentration of the substance in the solution
• 1 0 is the length of the optical path in the solution sample.
• the object of the present invention is to solve the above problems. To this end, it relates to a device for raising the level of a liquid containing a dissolved substance, in a cell, well or the like, characterized by a wall sleeve of variable thickness and open at its two ends, which is insertable into said cell or well.
• the sleeve is integral with an element comprising a plurality of such sleeves.
• This element can be a bar or a plate.
• It can include notching lines between the sleeves to allow their separation so as to use only part of the set of sleeves.
• the invention also relates to a plate with cells or multiple wells allowing for example the culture of cells or the microtitration of samples, characterized in that it is provided with a device corresponding to the preceding characteristics.
• This plate can be covered by a plate comprising 'on one side as many sleeves as wells in the plate.
• the invention also relates to the use of the above device or plate for measuring the optical density of a substance dissolved in this liquid.
• this device relates more particularly to the use of this device for the dosing of substances contained in a cell culture medium, these substances having for example been produced by cells in culture, or else having been released into the medium after solubilization of cells, these possibly having received an immunological labeling.
• Figure 1 is a perspective view of a sleeve inserted into a well.
• Figure 2 is a perspective view of a bar provided with such sleeves.
• Figure 3 is a perspective view similar to Figure 2 but showing individually separable sleeves of the bar.
• Figure 4 is a sectional view of the bar of Figure 2, passing through the axis of the sleeves, and illustrated in the mounted position on a row of wells.
• Figure 5 is a partial perspective view of a multiwell plate ready to receive a plate having on one side of the sleeves 1 according to the invention.
• a device according to the invention is, in general, constituted by a sleeve 1 which can be inserted into a cell or well 2 formed in an element 3 and which may contain a liquid in which any substance is dissolved.
• the sleeve 1 is open at its two ends to allow the passage of the light beam, and its wall 4 can have a variable thickness, which characterizes the caliber of the sleeve 1.
• the sleeve 1 once introduced into the well 2, will reduce the volume of this well as a function of the thickness of the wall 4 and will therefore allow the liquid to rise in the well 2 of the desired height depending on the size used.
• the sleeve 1, as well as the element 3 may be of any material such as for example a transparent plastic material for single use or reusable from one measurement to another after cleaning.
• a light beam passing through the well and the liquid it contains preferably in a direction substantially parallel to the axis of the well which is generally substantially cylindrical, will allow a measurement of the optical density of the substance dissolved in the liquid whose the height in the well will be large enough to provide the desired sensitivity, accuracy and efficiency of the measurement.
• the sleeve 1 may be integral with a support 5 having a plurality of such sleeves projecting from one side of said support.
• the support 5 can consist of a bar carrying a plurality of sleeves 1, the spacing of which corresponds to the spacing between the wells 2 of a line of wells made in the element 3.
• the bar-shaped support 5 can include notching lines 6 between the sleeves 1, which makes it easy to separate them from one another so as to insert them into such and such a well formed in the element 3.
• FIG. 4 shows a linear set of sleeves 1 belonging to a bar 5, as shown in FIG. 2, which line of sleeves is inserted into the wells 2 with corresponding spacing, formed in the element 3.
• the sleeves 1 can also be secured to a support in the form of a plate or plate 7, as can be seen in FIG. 5 where said sleeves 1 project from the same face of the plate 7.
• This plate 7 with associated sleeves may comprise as many sleeves 1 as there are cells or wells 2, provided in a plate P with multiple wells allowing, for example, cell culture, microtitration of samples, assay operations or even other uses.
• sleeves 1 can be used in only certain wells 2 of plate P, for example using a bar 5 with breakable or non-breaking sleeves, as seen in FIGS. 2 and 3 .
• the material and the wall thickness of the sleeves 1 can be any, as well as the geometrical position of the sleeves on a plate, a bar or any other support.

Landscapes

• Physics & Mathematics (AREA)
• Health & Medical Sciences (AREA)
• Life Sciences & Earth Sciences (AREA)
• Chemical & Material Sciences (AREA)
• Analytical Chemistry (AREA)
• Biochemistry (AREA)
• General Health & Medical Sciences (AREA)
• General Physics & Mathematics (AREA)
• Immunology (AREA)
• Pathology (AREA)
• Optical Measuring Cells (AREA)
• Apparatus Associated With Microorganisms And Enzymes (AREA)

Applications Claiming Priority (3)

Publications (1)

Family

ID=9454121

Family Applications (1)

Country Status (5)

Families Citing this family (3)

Family Cites Families (3)

• 1993
  • 1993-12-20 FR FR9315314A patent/FR2714183B1/fr not_active Expired - Fee Related
• 1994
  • 1994-12-14 EP EP95904560A patent/EP0685063A1/de not_active Withdrawn
  • 1994-12-14 AU AU13192/95A patent/AU1319295A/en not_active Abandoned
  • 1994-12-14 WO PCT/FR1994/001467 patent/WO1995017661A1/fr not_active Application Discontinuation
  • 1994-12-14 JP JP7517222A patent/JPH08510558A/ja active Pending

Non-Patent Citations (1)

Also Published As

Similar Documents

Legal Events