EP0650526A4 - Formation of triple helix complexes of single stranded nucleic acids using nucleoside oligomers. - Google Patents

Formation of triple helix complexes of single stranded nucleic acids using nucleoside oligomers.

Info

Publication number
EP0650526A4
EP0650526A4 EP92921942A EP92921942A EP0650526A4 EP 0650526 A4 EP0650526 A4 EP 0650526A4 EP 92921942 A EP92921942 A EP 92921942A EP 92921942 A EP92921942 A EP 92921942A EP 0650526 A4 EP0650526 A4 EP 0650526A4
Authority
EP
European Patent Office
Prior art keywords
formation
nucleic acids
single stranded
stranded nucleic
triple helix
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP92921942A
Other languages
German (de)
French (fr)
Other versions
EP0650526A1 (en
Inventor
O Paul On-Pong Ts
Thomas Henry Adams
Lyle J Arnold Jr
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Johns Hopkins University
Genta Inc
Original Assignee
Johns Hopkins University
Genta Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johns Hopkins University, Genta Inc filed Critical Johns Hopkins University
Publication of EP0650526A1 publication Critical patent/EP0650526A1/en
Publication of EP0650526A4 publication Critical patent/EP0650526A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6839—Triple helix formation or other higher order conformations in hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/15—Nucleic acids forming more than 2 strands, e.g. TFOs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/15—Nucleic acids forming more than 2 strands, e.g. TFOs
    • C12N2310/152—Nucleic acids forming more than 2 strands, e.g. TFOs on a single-stranded target, e.g. fold-back TFOs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/312—Phosphonates
    • C12N2310/3125—Methylphosphonates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/321—2'-O-R Modification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/33—Chemical structure of the base
    • C12N2310/334—Modified C
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3511—Conjugate intercalating or cleaving agent
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/352—Nature of the modification linked to the nucleic acid via a carbon atom
    • C12N2310/3521—Methyl
EP92921942A 1991-10-07 1992-10-05 Formation of triple helix complexes of single stranded nucleic acids using nucleoside oligomers. Withdrawn EP0650526A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US77208191A 1991-10-07 1991-10-07
US772081 1991-10-07
PCT/US1992/008458 WO1993007295A1 (en) 1991-10-07 1992-10-05 Formation of triple helix complexes of single stranded nucleic acids using nucleoside oligomers

Publications (2)

Publication Number Publication Date
EP0650526A1 EP0650526A1 (en) 1995-05-03
EP0650526A4 true EP0650526A4 (en) 1998-02-04

Family

ID=25093855

Family Applications (1)

Application Number Title Priority Date Filing Date
EP92921942A Withdrawn EP0650526A4 (en) 1991-10-07 1992-10-05 Formation of triple helix complexes of single stranded nucleic acids using nucleoside oligomers.

Country Status (7)

Country Link
EP (1) EP0650526A4 (en)
JP (1) JPH07501936A (en)
AU (2) AU2785292A (en)
CA (1) CA2119890A1 (en)
IL (1) IL103311A0 (en)
NZ (1) NZ244606A (en)
WO (1) WO1993007295A1 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996018732A2 (en) * 1994-12-15 1996-06-20 Board Of Trustees Of The University Of Illinois Sequence-specific inhibition of dna synthesis by triplex-forming oligonucleotides
US6274313B1 (en) 1996-03-21 2001-08-14 Pioneer-Hybrid International, Inc. Oligonucleotides with cationic phosphoramidate internucleoside linkages and methods of use
US6331617B1 (en) 1996-03-21 2001-12-18 University Of Iowa Research Foundation Positively charged oligonucleotides as regulators of gene expression
US5734040A (en) * 1996-03-21 1998-03-31 University Of Iowa Research Foundation Positively charged oligonucleotides as regulators of gene expression
US5853993A (en) * 1996-10-21 1998-12-29 Hewlett-Packard Company Signal enhancement method and kit
US7244732B2 (en) * 2003-06-20 2007-07-17 Koronis Pharmaceuticals, Incorporated Prodrugs of heteroaryl compounds
US7250416B2 (en) 2005-03-11 2007-07-31 Supergen, Inc. Azacytosine analogs and derivatives
US7700567B2 (en) 2005-09-29 2010-04-20 Supergen, Inc. Oligonucleotide analogues incorporating 5-aza-cytosine therein
KR102004559B1 (en) 2011-08-30 2019-07-26 아스텍스 파마수티컬스, 인크. Decitabine derivative formulations
BR112018000054A2 (en) 2015-07-02 2018-09-04 Otsuka Pharmaceutical Co., Ltd. lyophilized pharmaceutical compositions
SG11202000932XA (en) 2017-08-03 2020-02-27 Otsuka Pharma Co Ltd Drug compound and purification methods thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990015884A1 (en) * 1989-06-19 1990-12-27 The Johns Hopkins University Formation of triple helix complexes of double stranded dna using nucleoside oligomers
WO1992010590A1 (en) * 1990-12-10 1992-06-25 Gilead Sciences, Inc. Inhibition of transcription by formation of triple helixes

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4358535A (en) * 1980-12-08 1982-11-09 Board Of Regents Of The University Of Washington Specific DNA probes in diagnostic microbiology
CA2006008C (en) * 1988-12-20 2000-02-15 Donald J. Kessler Method for making synthetic oligonucleotides which bind specifically to target sites on duplex dna molecules, by forming a colinear triplex, the synthetic oligonucleotides and methods of use

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990015884A1 (en) * 1989-06-19 1990-12-27 The Johns Hopkins University Formation of triple helix complexes of double stranded dna using nucleoside oligomers
WO1992010590A1 (en) * 1990-12-10 1992-06-25 Gilead Sciences, Inc. Inhibition of transcription by formation of triple helixes

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
HÉLÈNE C: "The anti-gene strategy: Control of gene expression by triplex-forming-oligonucleotides", ANTI-CANCER DRUG DESIGN, vol. 6, 1991, pages 569 - 584, XP002047547 *
JAYASENA S D ET AL: "OLIGONUCLEOTIDE-DIRECTED TRIPLE HELIX FORMATION AT ADJACENT OLIGOPURINE AND OLIGOPYRIMIDINE DNA TRACTS BY ALTERNATE STRAND RECOGNITION", NUCLEIC ACIDS RESEARCH, vol. 20, no. 20, 1 January 1992 (1992-01-01), pages 5279 - 5288, XP002000132 *
ONO A ET AL: "Triplex formation of oligonucleotides containing 2'-O-methylpseudoisocytidine in substitution for 2'-deoxycitidine", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 113, 1991, pages 4032 - 4033, XP002047549 *
See also references of WO9307295A1 *
WELLS RD ET AL: "The chemistry and biology of unusual DNA structures adopted by oligopurine-oligopyrimidine sequences", FASEB JOURNAL, vol. 2, 1988, pages 2939 - 2949, XP002047548 *

Also Published As

Publication number Publication date
NZ244606A (en) 1997-07-27
CA2119890A1 (en) 1993-04-15
AU2488197A (en) 1997-09-04
WO1993007295A1 (en) 1993-04-15
AU2785292A (en) 1993-05-03
EP0650526A1 (en) 1995-05-03
IL103311A0 (en) 1993-03-15
JPH07501936A (en) 1995-03-02

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