EP0644754A4

EP0644754A4 - Substance that can stop bleeding in surgical, medical and dental uses/substance powder 001 that can stop bleeding during surgery, after surgery, medicine, dentistry or any bleeding due to any causes.

Info

Publication number: EP0644754A4
Application number: EP92901379A
Authority: EP
Inventors: Leslie Binshyang Soong
Original assignee: Individual
Current assignee: Individual
Priority date: 1990-12-06
Filing date: 1991-12-05
Publication date: 1995-12-27
Also published as: EP0644754A1; AU9103491A; AU662505B2; CA2095439C; WO1992010999A1; CA2095439A1; NO930858D0

Abstract

A method for stopping bleeding is described. The method entails applying to the site of bleeding powdered Vitamin C.

Description

SUBSTANCE THAT CAN STOP BLEEDING IN SURGICAL,MEDICAL AND DENTAL

USES/SUBSTANCE POWDER 001 THAT CAN STOP BLEEDING DURING SURGERY,

AFTER SURGERY, MEDECINE, DENTISTRY OR ANY BLEEDING DUE TO ANY CAUSES

SPE_CIFICATION OF INVENTION

(a) Title of the Invention.

The substance powder 001 that can stop (stanch) bleeding in surgical, postsurgical , medical and dental uses or any bleeding due to any causes by topical administration or the substance powder 001 that has very potent hemoεtatic action _can be administered topically to stop bleeding during surgery, after surgery, medicine by inqestion, dental surgery, extraction of teeth, and any injury or due to any causes for any normal and pathological ρpr^«?nn^«?.

(b) Cross-References to Related Applications.

The early literature on scurvy due to vitamin r riefioeπry wa reviewed by Bourne in 1944, he shoved hieroglyphs from Egypti papyri as convincing evidence that this disease, charaterized by bleeding gums and hemorrhages in the skin, existed about 3000 years ayo. Λ sudden pain seized their feet, and leys; immediately afterwards the gums and teeth were attacked by a sort of gangrene. The bones of the legs become horribly black. The legs shrivelled up and became covered with black spots. It as a sure sign of death when the nose began to bleed. ( Hourne, G,H, 1944, Proc. K. Spc. Me . , 37, 51? ) PΛTNΛIK 1971 stated that the concentration of ascorbic acid has been reported as decreaεingvith age in the rat eye lens, adrenal, brain, testis, and blood. ( Patnaik, B.K. 1971, Gerontologia , 17, 122 ) Purpura (extensive bruising ) and hemorrhages under the tongue are common in elderly people. Tattersal and Seville 1950 reported purpura in 60 out of 009 elderly patients admitted to hospital; the bruises were most commonly seen on the anterior aspects of their arms and fore- ornv. and on tr.e backs of their hands, noth p-it ientj. an⁷ the controls were ascorbate deficient, as _ju ged by ascorr.:r acid loading tests; skin biopsies from those vith purσura sho ed degeneration of tne collagen fibres rf the derππs. Tatttisa (c) Background of the Invention.

(1) THIS invention pertains to the field of disorder of hemσstasis, which is further classified into evaluation of hemostatic function, platelet disorders, coagulation factor disorders and thromboe bolic disorders. However, the blood coagulation mechanism of ascorbic acid which - was administered topically does not fall into either intrinsic or extrinsic pathway cascade, since it works the same for hemostaεiε in normal situation as in chronic- hepatitis, colon cancer, and hemophilia patients in whom the coagulation factors 2, 7, 9, 10 in the liver dise and factor 8 or 9 in the hemophilia A or B cannot be produ (2) The present methods available to stop bleeding in surger are cautery, epinephrine injection, pressure and some fiber foam. The cautery can cause tissue necrosis in some cases. The epinephrine, pressure and fiber foam can not work for bleeding tendency patients, and fiber foam can cause tissue hemotoma, nor can they work for diffuse capillary bleeding and oozing, while ascorbic acid does not cause any tissue necrosis and works particularly >r*\ \ for diffuse capillary bleeding and any traditionally un¬ controllable bleeding tendency patients such as for patient with liver diseases, hemophilia and rectal anal cancer and for postoperation bleeding tendency nf the kinds of aforementioned patients. The ascorbic acid can facilitate wound healing also ( Oral Surg 1982 Mar: 53(3): 231-6 ) by increasing type 1 procollagen mRNΛ in the regulation of collagen synthesis ( Atherosclerosis 1982 Jan: 41(1): 15-9 ) (d) Explanation of Invention / Summary.

Substance powder 001 represents ascorbic acid powder that i^c obtained by grinding the vitamin C tablets into powder. This powder has a very potent hemostatic action to stop bleeding wnen spraying it topically on the bleeding area for any with the blood, the black tar gel like material is formed, and later becomes solid. The amount of the powder that is needed to react completely with the blood is proportional to the amount of the blood that comes out of the blood vessels. In rats, one capillary, one venous and one femoral artery bleeding were stanched within one second, if sufficien amounts of the powder of ascorbic acid were applied. The arte bleeding needs much more this powder of interest. In the test tube conditions, it requires 0.1568 g of this substance powde to coagulate ( make the blood black and not move in the tube 1 ml of human blood in 6 minutes. This is the same for normal colon cancer a d hemophilia patients; 1 ml of normal blood needs 0.1568 g of ascorbic acid powder, 1 ml of colon cancer blood needs 0.1568 g of ascorbic acid powder, and 1 ml of hemophilia blood needs 0.1568 g of ascorbic acid powder. Suff substance powder 001 is particularly useful to help stop blee in the case of diffuse capillary oozing and mistakenly cut of artery or arteries that cannot be found during massive bleedi bleeding so as to save the patients' lives.

The present methods available to stop bleeding in surgery as previously mentioned are cautery, epinephrine injection, press and some fiber foam. The cautery can cause tissue necrosis in some cases. The epinephrine, pressure and fiber foam can not cannot work for bleeding tendency and diffuse capillary oozi patients, while ascorbic acid powder does not cause any tissu necrosis and particularly works well for diffuse capillary bl and oozing and any traditionally uncontrollable bleeding tend patients such as for patients with liver diseases, hemophilia and colon rectal or other cancer bleeding conditions and for postoperation bleeding tendency of the kinds of aformentioned patients. The ascorbic acid also facilitate wound healing ( O Surg. 1982 Mar: 53 (3j: 231-6 ) by increasing type 1 procollag mRNA in the regulation of collagen synthesis ( Atherosclerosis 1982 Jan: 41(1): 15-9 ) and when the vitamin C is absorbed into the system, it is good for health by making the leucocytes stronge and slowing the aging process etc, ( Patnaik and Kanungo 1964, Gerontologia, 10, 155 ) as long as it is within the optimal dose such as 1 g, However, at the end of operation before closure, the excessive black tar qel like material can be wiped away and washed with Ringer's feblutJion*

( e) Description of the preferred embodiments of the invention:

As already mentioned previously, the substance powder ool is made by grinding the pure vitamin C tablets into powder. Application of this powder topically to the bleeding area is betber'by spraying the powder topically on the bleeding area. The amount of vitamin C powder needed to spray is proportional to the amount of blood coming out of the vessels. In test tube conditions, it requires 0.1568 g of this^* vitamin C powder to coagulate 1 ml of human blood. This _λiε the same for normal, liver diseases, cancer, and hemophilia patients. The black coagulation takes place in 6 minutes by shaking the test tube. The description of this sβtuation is made in the evidence of animal ( rats ) experiments and clinical evidence as follows: (f) Rats Experiments

1) Sufficient substance powder 001 can stop massive bleeding of femoral artery within one second when femoral artery is cut off. ( Photograph # 1 : massive bleeding when the femoral artery is cut off ) ( Photogrph # 2: bleeding stops within one second when sufficient powder of interest was poured topically into the bleeding area, in this case of massive active bleeding T did pouring instead of spraying. The amount of the powder needed is proportional to the amount of blood coming out of the vessel. It varies with the rats. Usually 0.1344 g is sufficient to stop bleeding for the femoral artery of the rat which is cut off vithin one second. In the photograph # 2, the black tar gel like material was formed in one second as soon as the powder contacts with the blood . Those white spots indicate the powder that is not yet mixed up with the blood. The black gel forms first on l.he surface of the pond of blood, that stop the fluid moving. If stirring the blood to break through the surface film like πtru that keep the blood fluid from moving when the powder is insuf it will bleed again, but slower than ( much slower ) before • t pouring the powder into the bleeding area; but after adding sufficient powder into the blood, stirring makes the powder molecules well saturated with the hemoglobin and plasma protei molecules and thus forming the concentrated black sticky gel li structure. After 5-6 minutes, when most of the sticky tar like structure is wiped away, it appears there is no bleeding at all. ( Photograph tf 3 ). In the case of capillary bleeding in which one capillary iε cut off, fibout 0. 0112 g of this powder can stop the bleeding within one second, ( Photograph # 4 ). After 1 minute count from adding the powder, it appears there is no more bleeding while wiping away the black tar qel like structure, while in the case of one vein cut off, it needs about 0.0224 g of the powder to stop bleeding, and about after 1 1/2 minutes, it appears there is no more bleeding when the black coagulation is wiped away. ( Photograph t 5 ) . The nostril was cut slightly to bleed ( Photograph # 6 ) in rat the bleeding stop within one second when applying this powder 0.0037 g topically into the bleeding nostril, ( Photograph # 7 ). After injection of histamin into the rat, it induces secretion of gastric acid, open up the stomach ( Photograph * G ), cut the mucosa layer to bleed ( Photograph # 9 ), applying the powder topically to the bleeding area, the black coagulatio formed, but slower than normal, since the secretion dilute the black coagulation. ( Photograph # 10) hup this powder can treat the gastric ulcer bleeding when it is inαested orally. Clinical Evidence.

This powder also help stop massive bleeding in surgery, no matter what kind of diseases the patients suffered from.

Case 1. Age 32, male, scalp tumor excision, massive bleeding when the tumor vas exciεed. ( Photograph # I'l ) Sufficient spraying this powder into the bleeding area also help stop bleerling before it was closed by suture. ( Photo¬ graph # 1>2 ) Case 2. Age 40, female, acute renal failure, Λ-V fistula formation operation, massive bleeding ( Photograph * 1 _ ) The operation of stopping bleeding consiεts of combining the powder with press with gauze. When applying this powder to the bleeding area, those that look black in color indicate the black blood coagulation ( Photograph -ft lj_) ), while those that.are white in color are those the powder does not get mixed up with the blood ( Photograph 1'5 ). At the end of the operation, most of black blood coagulation was wiped out with 4x4 gau/.e, and rinsπrl with Ringer's solution ( Photograph # 1'6 ). It took totally 5 minuteε to stop the bleeding completely. The patient's conditions are especially well, better than without using this powder. Case 3_* Age 40, male, skin contracture on the palm, which needs to be removed, and it requires skin graft.-

When the skin graft is removed from the hand,massive bleedi occurs ( Photograph # K7 ) . Qn applying sufficient powder to the bleeding area topically, it appears black coagulation within one second. Bleeding stops nt the same time ac the black coagulation forms. Those that appears white indicate the powder not yet mixed up with the blood. ( Photograph 4 18 ); those that appears more black area indicate the ππre powder mixed up with the blood (Photograph # 19 ), Suture going on ( Photograph * 2 ) Case 4 . Ληe 70 , f ema le , pa raana l abscess exci si on , bl eed i nq when i t i s opened up ( Photograph # 21 ) . When spra yi the powder to the bleeding area, it stop bleeding by forming black blood coagulation immediately. Those that appears white are the powder that are not mixed up with the blood. ( Photograph # 212), Suture going on ( Photograph # 23 ) . Case 5. Age 50, male, paraanal abscess excision. m---εive bleeding when it is opened up ( Photograph * 24 ). On spraying the powder topically to the bleedin area, the black color is formed im edi^ely because o the blood coagulation ( Photograph * 25 ) * The white color is the powder instantly not mixed up- with the blood yet. ( Photograph # 2^r ), lastly, in^p.Tt the long gauze into the anus. ( Photograph <t 21 ) Case f». Age 30, male, exiraction of tooth, the gum injected b ( Photograph t, 2 _ ). Applying this powder topically to the bleeding area, the bleeding stop within one sec and black blood coagulation forms within one second ( Photograph # 2§ ) . Wipe out the black coagulation in 10 seconds, stop bleeding still persisted ( Photo¬ graph * 3'0 ) Case 7. Age 60, female, chronic hepatitis, has bleeding tende extinction of teeth cause massive bleeding ( Photograp # 3'i ) On applying this powder to the socket of the gu the black coagulation forms within one second, the ble of Ihis socket εic b within one second a~cordingly . ( Photograph * 32 ) Case 0. myself, finger little scratched, slightly bleeds

( Pht.iograph * 33 ) . Upon contact with the powder, l he black color forms within one second or shorter ( Photo PCT/US91/0920

In test, tube con il.ion as mentioned previously, ] _m] of human b¹.nor! requires 0.15C>fl q of powder to form Ma<-k bloori coa- qulatton by shaking the tube with hand or εtirer machine.

Tl. Lake;; G minutes t.o form the immobile black coaqυlatinπ in the tost t.uhe for normal, liver di.season, colon ranker and hemophilia hlood respectively. ( Photograph ft 3 _ normal blood, colon cancer Photograph # 3* ,, and hemophilia blood

P_huto_'jraph i_\ 3'7 ) This indicates that, the mechanism of the coagulation by this powder does not involve the coagulation factors. The hemophilia blood ( both prior to adding the powder and after adding powder) are still stored in the freezer here.

This patient's name: Su Jean Wang, Age 25, ma.le, 0.1.

# S 121745233, address: No 5, Su Ming Rd., Lane 100

Nan Tze District, Kaohsiung City / lastly, Stops bleeding in tonsillectomy rat within one second by topical use of th (f). Claims of Utilities of ascorbic acid powder. j

1. Stops bleeding in the nose bleeding in humans within one second, experi nced in my 6 years old dauqhl.er by applying this powder topically χ_ _o her bleeding nostril.

' . Stops hlocdinq in any ijury due to any c.-πise, this also be useful for wound bleeding in the war or combat, zone.

^'; . S o bleediπq in bleeding tendency patients in surgery or after surgery. Help stop bleeding in Λ-V fistula formation surgery in preparation for hemodialysis.

4. Stops bleeding in massive bleeding in scalp tumor excision. ^r-. Sto ._'* lileediruj in bleeding tendency in paraπal. nbsress orci ion.

6. Stops bleeding in postoperation ( after surq ry ) bleeding Ϊti nni-itii'nm vrnmrl bleeding after rectal anal cancer surgery.

7. Stops bleeding in teeth extraction within one second in bleeding tendency patients when applying this powder topically to the bleeding socket of the gum.

8. Stops bleeding in the hemophilia patients due to injury when applying the powder topically to bleedinq area suf iciently.

9. Stops bleeding in Ob F» Gyn surgery in bleeding tendency

Claims

patients when applying this powder sufficiently to the bleeding area.

IC. Stops bleeding due to injury or bleeding due to any caus

11. Stops gastric ulcer bleeding when ingestion of this powd about 1 g / day orally.

12. Help stop and prevent internal bleeding when taking 1 g of this powder daily orally.

1?. ^«-np bleeding and help stop bleeding in tonsillectomy

,., . and after tonsillectomy bleeding. . __ ^