EP0643768A4 - Sequences d'oligonucleotides et animaux transgeniques transfectes avec celles-ci et presentant une sensibilite reduite aux analgesiques narcotiques. - Google Patents

Sequences d'oligonucleotides et animaux transgeniques transfectes avec celles-ci et presentant une sensibilite reduite aux analgesiques narcotiques.

Info

Publication number
EP0643768A4
EP0643768A4 EP93912092A EP93912092A EP0643768A4 EP 0643768 A4 EP0643768 A4 EP 0643768A4 EP 93912092 A EP93912092 A EP 93912092A EP 93912092 A EP93912092 A EP 93912092A EP 0643768 A4 EP0643768 A4 EP 0643768A4
Authority
EP
European Patent Office
Prior art keywords
transgenic animals
reduced sensitivity
seq
narcotic analgesics
oligonucleotide sequences
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP93912092A
Other languages
German (de)
English (en)
Other versions
EP0643768A1 (fr
Inventor
Nancy M Lee
Horace H Loh
David Lippman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DUZ PARTNERSHIP
Original Assignee
DUZ PARTNERSHIP
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DUZ PARTNERSHIP filed Critical DUZ PARTNERSHIP
Publication of EP0643768A1 publication Critical patent/EP0643768A1/fr
Publication of EP0643768A4 publication Critical patent/EP0643768A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1138—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against receptors or cell surface proteins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Environmental Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Veterinary Medicine (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

On a isolé et séquencé des clones (SEQ ID NO:1, SEQ ID NO:2, et SEQ ID NO:3). On a formé une structure non codante à partir d'une partie de la SEQ ID NO:1 qui bloque la liaison opioïde dans des cellules, et on l'a utilisée pour produire des animaux transgéniques présentant une sensibilité réduite aux analgésiques narcotiques. La séquence d'oligonucléotides introduite dans les animaux à l'état embryonnaire était la première parmi environ 500 paires de base mais son sens était inversé. Les structures d'oligonucléotides de cette invention peuvent également être utilisées comme sondes ou comme agents thérapeutiques ou diagnostiques.
EP93912092A 1992-04-10 1993-03-29 Sequences d'oligonucleotides et animaux transgeniques transfectes avec celles-ci et presentant une sensibilite reduite aux analgesiques narcotiques. Withdrawn EP0643768A4 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US86667792A 1992-04-10 1992-04-10
PCT/US1993/002913 WO1993021309A1 (fr) 1992-04-10 1993-03-29 Sequences d'oligonucleotides et animaux transgeniques transfectes avec celles-ci et presentant une sensibilite reduite aux analgesiques narcotiques
US866677 1997-05-30

Publications (2)

Publication Number Publication Date
EP0643768A1 EP0643768A1 (fr) 1995-03-22
EP0643768A4 true EP0643768A4 (fr) 1996-11-27

Family

ID=25348147

Family Applications (1)

Application Number Title Priority Date Filing Date
EP93912092A Withdrawn EP0643768A4 (fr) 1992-04-10 1993-03-29 Sequences d'oligonucleotides et animaux transgeniques transfectes avec celles-ci et presentant une sensibilite reduite aux analgesiques narcotiques.

Country Status (6)

Country Link
EP (1) EP0643768A4 (fr)
JP (1) JPH08501442A (fr)
AU (1) AU677200B2 (fr)
CA (1) CA2117756A1 (fr)
NO (1) NO943808L (fr)
WO (1) WO1993021309A1 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020061554A1 (en) 1992-08-13 2002-05-23 Evans Christopher J. Orphan opioid receptor and recombinant materials for its production
WO1994004552A1 (fr) * 1992-08-13 1994-03-03 The Regents Of The University Of California Genes de recepteurs opioides
US6258556B1 (en) * 1993-02-26 2001-07-10 The United States Of America As Represented By The Department Of Health And Human Services cDNA and genomic clones encoding human μ opiate receptor and the purified gene product
US7083932B1 (en) 1995-08-15 2006-08-01 Euroscreen, S.A. Nucleic acid molecules encoding peptides having pronociceptive properties
EP0845034B1 (fr) * 1995-08-15 2003-03-05 Euroscreen S.A. peptide presentant des proprietes pronociceptives
FR2750825B1 (fr) * 1996-07-15 2002-08-23 Centre Nat Rech Scient Animal transgenique non humain dans lequel l'expression de l'un au moins des genes codant pour les recepteurs aux opiaces est modifiee

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5087571A (en) * 1984-06-22 1992-02-11 President And Fellows Of Harvard College Method for providing a cell culture from a transgenic non-human mammal

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
ANN D.K. ET AL.: "Specific reduction of delta opioid receptor binding in transfected NG108-15 cells", JOURNAL OF BIOLOGICAL CHEMISTRY (MICROFILMS), vol. 267, no. 11, 15 April 1992 (1992-04-15), MD US, pages 7921 - 7926, XP000602135 *
CHAKRABORTI, A. ET AL.: "Genetic mapping of opioid binding protein gene(s) to mouse chromosome 9", MAMMALIAN GENOME, vol. 4, no. 3, 1993, pages 179 - 182, XP000602417 *
GOVITRAPONG, P. ET AL.: "Transfection of NG108-15 cells with antisense opioid-binding cell adhesion molecule cDNA alters opioid receptor G-protein interaction", JOURNAL OF BIOLOGICAL CHEMISTRY (MICROFILMS), vol. 268, no. 24, 25 August 1993 (1993-08-25), MD US, pages 18280 - 18285, XP000602144 *
LANE, C.M. ET AL.: "Regulation of an opioid-binding protein in NG108-15 cells parallels regulation of delta opioid receptors", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 89, no. 23, 1 December 1992 (1992-12-01), WASHINGTON US, pages 11234 - 11238, XP000602145 *
LIPPMAN, D.A. ET AL.: "Opioid-binding cell adhesion molecule (OBCAM)-related clones from a rat brain cDNA library", GENE, vol. 117, no. 2, 15 August 1992 (1992-08-15), AMSTERDAM NL, pages 249 - 254, XP000602047 *
See also references of WO9321309A1 *

Also Published As

Publication number Publication date
NO943808L (no) 1994-12-06
AU677200B2 (en) 1997-04-17
JPH08501442A (ja) 1996-02-20
NO943808D0 (no) 1994-10-07
CA2117756A1 (fr) 1993-10-28
EP0643768A1 (fr) 1995-03-22
WO1993021309A1 (fr) 1993-10-28
AU4276993A (en) 1993-11-18

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