EP0414809A4 - Plant defense gene regulatory elements - Google Patents

Plant defense gene regulatory elements

Info

Publication number
EP0414809A4
EP0414809A4 EP19890906880 EP89906880A EP0414809A4 EP 0414809 A4 EP0414809 A4 EP 0414809A4 EP 19890906880 EP19890906880 EP 19890906880 EP 89906880 A EP89906880 A EP 89906880A EP 0414809 A4 EP0414809 A4 EP 0414809A4
Authority
EP
European Patent Office
Prior art keywords
promoter
group
promoters
plant
encode
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP19890906880
Other languages
French (fr)
Other versions
EP0414809A1 (en
Inventor
Christopher John Lamb
Michel Dron
Vincent Paul Mary Wingate
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Salk Institute for Biological Studies
Original Assignee
Salk Institute for Biological Studies
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Salk Institute for Biological Studies filed Critical Salk Institute for Biological Studies
Publication of EP0414809A1 publication Critical patent/EP0414809A1/en
Publication of EP0414809A4 publication Critical patent/EP0414809A4/en
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12N9/1037—Naringenin-chalcone synthase (2.3.1.74), i.e. chalcone synthase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8239—Externally regulated expression systems pathogen inducible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

Definitions

  • the present invention discloses plant defense gene regulatory elements that can be "turned on”, induced or otherwise activated by exogenous elicitors. .-+ ⁇

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Communicable Diseases (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention discloses plant defense gene regulatory elements that can be ''turned on'', induced or otherwise activated by exogenous elicitors.

Description

International Bureau
INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT
(51) International Patent Classification 4 (11) International Publication Number: WO 89/1
C07H 15/12, G01N33/00 Al C12N 15/00, G01N33/48 (43) International Publication Date: 14 December 1989 (14.
(21)InternationalApplication Number: PCT/US89/02150 (72) Inventors; and
(75) Inventors/Applicants (for US only) : LAMB, Christ
(22) International Filing Date: 18 May 1989 (18.05.89) John [GB/US]; 6444 Farley Drive, San Diego 92122 (US). DRON, Michel [FR/FR]; 7, resi
(30) Priority data: Foncs-Fanettes, F-91190 Gif (FR). WINGATE, Vi
197,122 20 May 1988 (20.05.88) US Paul, Mary [US/US]; Steptoe Village, P-109, Pul 343,445 26 April 1989 (26.04.89) US WA 99163 (US).
(60) Parent Applications or Grants (74)Agents: WATT, Phillip, H. et al.; Fitch, Even, Ta
(63) Related by Continuation Flannery, Room 900, 135 South LaSalle Street, Chi US 197,122 (CIP) IL 60603 (US).
Filed on 20 May 1988 (20.05.88) US 343,445 (CIP) (81) Designated States: AT (European patent), BE (Eur Filed on 26 April 1989 (26.04.89) patent), CH (European patent), DE (European pa FR (European patent), GB (European patent), IT (
(71) Applicant (for all designated States except US): THE SALK pean patent), JP, LU (European patent), NL (Euro INSTITUTE FOR BIOLOGICAL STUDIES [US/US]; patent), SE (European patent), US, US. 10010 North Torrey Pines Road, La Jolla, CA 92037 (US). Published
With internationalsearch report.
Before the expiration of the time limit for amendin claims and to be republished in the event ofthe rece amendments.
(54)Title: PLANT DEFENSE GENE REGULATORY ELEMENTS
111 i CAT I I N0S3'i_ _
(57) Abstract
The present invention discloses plant defense gene regulatory elements that can be "turned on", induced or otherwise activated by exogenous elicitors. .-+<
!
FOR THE PURPOSES OF INFORMATION ONLY
Codes used to identify States party to the PCT on the front pages of pamphlets publishing international applications under the PCT.
AT Austria FI Finland ML Mali
AU Australia FR France MR Mauritania
BB Barbados GA Gabon MW Malawi
BE Belgium GB United Kingdom NL Netherlands
BF Burkina Fasso HU Hungary NO Norway
BG Bulgaria IT Italy RO Romania
BJ Benin JP Japan SD Sudan
BR Brazil KP Democratic People's Republic SE Sweden
CF Central African Republic ofKorea SN Senegal
CG Congo KR Republic ofKorea SU Soviet Union
CH Switzerland U Liechtenstein TD Chad
CM Cameroon LK Sri Lanka TG Togo
DE Germany, Federal Republic of LU Luxembourg US United States of America
DK Denmark MC Monaco
ES Spain MG Madagascar

Claims

AMENDED CLAIMS
[received by the International Bureau on 5 January 1990 (05.01.90); original claims 7,9 and 13 amended; other claims unchanged (3 pages)]
1. A substantially pure plant defense gene promoter that directs stress-regulated expression of an operatively linked gene, said promoter selected from the group consisting of: promoters for the plant genes that encode the phenylpropanoid biosynthetic enzymes phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), and 4-coumarate:CoA ligase (4CL), plus promoters for the plant genes that encode the cell wall hydroxyproline-rich glycoproteins.
2. A substantially pure functional domain in the promoter of Claim 1, wherein said functional domain is selected from the group consisting of (1) an elicitor-regulated activator domain, and (2) an upstream silencer domain.
3. A DNA sequence comprising: ACCAATTATTGGTTACTAAATTTAACAGTGAATGAATGAATGAGTTATA.
4. A DNA sequence comprising: CACGTGATACTCACCTACCCTAC. 5. A DNA sequence having substantial sequence homology with the DNA sequences in any of Claims 3 or 4.
6. Chimeric plasmids selected from the group consisting of pCHC1 and pCHC2.
7. A chimeric gene cassette (CGC) comprising: (a) a promoter selected from the group consisting of promoters for the plant genes that encode phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), 4-coumarate:CoA ligase (4CL), and promoters for the plant genes that encode cell wall hydroxyproline-rich glycoproteins; wherein said promoter is operatively linked to (b) at least one reporter gene; and (c) a 3' terminator.
8. A chimeric gene cassette (CGC) according to Claim 7 wherein said reporter gene is selected from the group consisting of chloramphenicol acetyItransferase (CAT) and beta glucuronidase (GUS) .
9. A chimeric gene cassette (CGC) comprising: (a) a promoter selected from the group consisting of promoters for the plant genes that encode phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), 4-coumarate:CoA ligase (4CL), and promoters for the plant genes that encode cell wall hydroxyproline-rich glycoproteins; wherein said promoter is 10 operatively linked to (b) at least one structural gene capable of being expressed in plants ; and (c) a 3 ' terminator.
10. A chimeric gene cassette (CGC) according to any of Claims 7 or 9 wherein said 3' terminator is selected from the group consisting of the 3' flanking region of the nopaline synthase (NOS) gene and the 3' flanking region of the octopine synthase (OCS) gene.
11. The reduced form of glutathione when used exogenously to activate plant defense genes.
12. A method for activating plant genes, said method comprising: applying reduced glutathione exogenously to plant material that contains a structural gene operatively linked to a promoter that is activated by reduced glutathione.
13. A method according to Claim 12 wherein said promoter is selected from the group consisting of promoters for the plant genes that encode phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), 4-coumarate:CoA ligase (4CL), and promoters for the plant genes that encode the cell wall hydroxyproline-rich glycoproteins.
14. Plant material engineered with human effort wherein said plant material contains the chimeric gene cassette (CGC) any of Claims 7 or 9.
15. Plant material according to Claim 14 wherein said plant material is selected from the group
EP19890906880 1988-05-20 1989-05-18 Plant defense gene regulatory elements Withdrawn EP0414809A4 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US19712288A 1988-05-20 1988-05-20
US197122 1988-05-20
US34344589A 1989-04-26 1989-04-26
US343445 1989-04-26

Publications (2)

Publication Number Publication Date
EP0414809A1 EP0414809A1 (en) 1991-03-06
EP0414809A4 true EP0414809A4 (en) 1991-10-09

Family

ID=26892568

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19890906880 Withdrawn EP0414809A4 (en) 1988-05-20 1989-05-18 Plant defense gene regulatory elements

Country Status (3)

Country Link
EP (1) EP0414809A4 (en)
JP (1) JPH03504921A (en)
WO (1) WO1989012059A1 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL100908A0 (en) * 1991-02-22 1992-11-15 Salk Inst Biotech Ind Invertase genes and their use
US5399680A (en) * 1991-05-22 1995-03-21 The Salk Institute For Biological Studies Rice chitinase promoter
WO1993007272A1 (en) * 1991-10-03 1993-04-15 Calgene Pacific Pty. Ltd. Transgenic plants
US5874626A (en) * 1993-05-20 1999-02-23 Purdue Research Foundation Osmotin gene promoter and use thereof
AR008231A1 (en) * 1996-06-13 1999-12-29 Univ Michigan State AN ISOLATED NUCLEIC ACID SEQUENCE, A VECTOR UNDERSTANDING IT, A TRANSGENIC PLANT CELL, A SEED OF SUCH A PLANT AND A METHOD TO REDUCE THE TRANSCRIPTION OF A DNA SEQUENCE IN A TRANSGENIC PLANT
AU9117598A (en) 1997-08-27 1999-03-16 Pioneer Hi-Bred International, Inc. Genes encoding enzymes for lignin biosynthesis and uses thereof
US6072103A (en) * 1997-11-21 2000-06-06 Calgene Llc Pathogen and stress-responsive promoter for gene expression
US6368837B1 (en) 1999-08-06 2002-04-09 E. I. Du Pont Nemours And Company Bioproduction of para-hydroxycinnamic acid
US6521748B2 (en) 1999-08-06 2003-02-18 E. I. Du Pont De Nemours And Company Polynucleotide encoding a mutant Rhodotorula glutinis tyrosine ammonia lyase polypeptide
WO2001012801A2 (en) * 1999-08-18 2001-02-22 Pioneer Hi-Bred International, Inc. Defense-related signaling genes and methods of use
WO2014100009A2 (en) 2012-12-19 2014-06-26 Monsanto Technology Llc Plant regulatory elements and uses thereof

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
CHEMICAL ABSTRACTS, vol. 104, 1986, page 163, abstract no. 62996d, Columbus, Ohio, US; D.J. CUMMINGS et al.: "Excision-amplification of mitochondrial DNA during senescence in Podospora anserina. DNA sequence analysis of three unique "plasmids"", & J. MOL. BIOL. 1985, 185(4), 659-80 *
CHEMICAL ABSTRACTS, vol. 92, 1980, page 353, abstract no. 212263n, Columbus, Ohio, US; B. HOVEMANN et al.: "Analysis of a Drosophila tRNA gene cluster", & CELL (CAMBRIDGE, MASS.) 1980, 19(4), 889-95 *
CURRENT COMMUNICATIONS IN MOLECULAR BIOLOGY: GENETIC IMPROVEMENTS OF AGRICULTURALLY IMPORTANT CROPS: PROGRESS AND ISSUES; MEETING, 1988, pages 31-36, Cold Spring Harbor Lab., New York, US; C.J. LAMB et al.: "Plant defense gene regulation" *
JOURNAL OF CELLULAR BIOCHEMISTRY, supplement 12C, 1988, UCLA SYMPOSIA ON MOLECULAR & CELLULAR BIOLOGY, ABSTRACTS OF THE 17TH ANNUAL MEETING, 28th February - 10th April 1988, page 221, abstract no. L702, Alan R. Liss, Inc., New York, US; M. DRON et al.: "Elicitor regulation of a plant defense gene promoter in electroporated protoplasts" *
JOURNAL OF CELLULAR BIOCHEMISTRY, supplement 12C, 1988, UCLA SYMPOSIA ON MOLECULAR & CELLULAR BIOLOGY, ABSTRACTS OF THE 17TH ANNUAL MEETINGS, 28th February - 10th April 1988, page 228, abstract no. L707, Alan R. Liss, Inc., New York, US; M.A. LAWTON et al.: "Nuclear protein binding to a Cis-acting silencer in a defense gene promoter" *
MOL. GEN. GENET., vol. 210, 1987, pages 219-233, Springer-Verlag; T.B. RYDER et al.: "Organization and differential activation of a gene family encoding the plant defense enzyme chalcone synthase in Phaseolus vulgaris" *
PLANT PHYSIOL., vol. 87, 1988, pages 206-210; V.P.M. WINGATE et al.: "Glutathione causes a massive and selective induction of plant defense genes" *
PROC. NATL. ACAD. SCI. USA, vol. 83, 1983, pages 5372-5376; J.R. ECKER et al.: "Inhibition of gene expression in plant cells by expression of antisense RNA" *
PROC. NATL. ACAD. SCI. USA, vol. 85, September 1988, pages 6738-6742; M. DRON et al.: "Glutathione and fungal elicitor regulation of a plant defense gene promoter in electroporated protoplasts" *
See also references of WO8912059A1 *

Also Published As

Publication number Publication date
EP0414809A1 (en) 1991-03-06
WO1989012059A1 (en) 1989-12-14
JPH03504921A (en) 1991-10-31

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