Classifications

• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
  • B01J10/00—Chemical processes in general for reacting liquid with gaseous media other than in the presence of solid particles, or apparatus specially adapted therefor
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
  • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
  • B01J19/18—Stationary reactors having moving elements inside
  • B01J19/1812—Tubular reactors
  • B01J19/1837—Loop-type reactors
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
  • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
  • B01J19/18—Stationary reactors having moving elements inside
  • B01J19/1868—Stationary reactors having moving elements inside resulting in a loop-type movement
  • B01J19/1875—Stationary reactors having moving elements inside resulting in a loop-type movement internally, i.e. the mixture circulating inside the vessel such that the upwards stream is separated physically from the downwards stream(s)
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
  • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
  • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
  • C12M29/08—Air lift
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
  • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
  • C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
  • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes

Definitions

• the invention relates to carrying out biotechnological processes by means of a multiphase system in a loop reactor, which system comprises an aqueous phase.
• Loop reactors are described in detail in "Biotechnology” by H.J. Rehm and G. Reed, vol. 2, “Fundamentals of Biochemical Engineering", VCH, Weinheim (1985), chapter 21 entitled “Biochemical Loop Reactors", pages 465-517. More particularly, in a loop reactor, at least one defined circulatory flow of a liquid medium present therein takes place and loop reactors have in principle either an "internal circulation” (see Figure 1a) or an “external circulation” (see Figure 1b).
• an organic solvent which is immiscible with water is understood to mean a solvent which is not miscible with water or soluble in water in all proportions so that a liquid multiphase system can be created.
• the method according to the invention differs in principle from the method described in the abovement ioned Swiss Patent 478,282.
• the reason is that, according to said Swiss patent, two liquids which are immiscible with each other are subjected to a mixing action by means of a stirring device, i.e. the stirring device provides the driving force for the mixing action, whereas, in the method according to the invention, the difference in density between the liquid components used functions as driving force.
• reaction systems according to the invention are very readily. usable in biocatalysis, use being made of organic media.
• organic media By replacing a portion of the aqueous phase by an organic solvent which is immiscible with water, it is possible, in addition to eliminating completely or partially the abovementioned disadvantages, also to obtain a number of additional advantages, namely: a displacement of reaction equilibria as a consequence of modified distribution of substrates and reaction products between the phases present, a lowering of the substrate/reaction product inhibition, an improved product and biocatalyst working up and a stabilization of the biocatalyst.
• an organic solvent which is immiscible with water and which has a lower density than water use is made of an organic solvent which is immiscible with water and which has a lower density than water.
• the possibility is, however, also available of using an organic solvent which is immiscible with water and which has a higher density than water.
• organic solvents which can be used in the method according to the invention are listed in the table below.
• Figure 2a illustrates an internal loop reactor which contains water (1) as the continuous phase and an organic solvent (2) having a density lower than water as the disperse phase.
• Figure 2b illustrates an external loop reactor which contains water (1) as the continuous phase and an organic solvent (2) having a density lower than water as the disperse phase.
• Figure 2c illustrates an internal loop reactor which contains water (3) as the disperse phase and an organic solvent (4) having a density higher than water as the continuous phase.
• Figure 2d illustrates an external loop reactor which contains water (3) as the disperse phase and an organic solvent (4) having a density higher than water as the continuous phase.
• Figure 3a illustrates an internal loop reactor which contains water (5) as the continuous phase and an organic solvent (6) having a density higher than water as the disperse phase.
• Figure 3b illustrates an external loop reactor which contains water (5) as the continuous phase and an organic solvent (6) having a density higher than water as the disperse phase.
• Figure 3c illustrates an internal loop reactor which contains water (7) as the disperse phase and an organic solvent (8) having a density lower than water as the continuous phase.
• Figure 3d illustrates an external loop reactor which contains water (7) as the disperse phase and an organic solvent (8) having a density lower than water as the continuous phase.
• Figure 4 shows a special embodiment of the method according to the invention in which use is made of two organic solvents which are immiscible with water, one (9) having a higher density and the other (10) a lower density than the water (11) present.
• the use of this three-phase system in a loop reactor with an "internal" circulation is in principle also possible.
• the loop reactor used for carrying out the method according to the invention may, if desired, be provided with solid particles such as immobilized catalyst particles and the like which are dispersed in the continuous phase or may be provided in another manner (see the abovementioned book entitled "Biotechnology” by H.J. Rehm and G. Reed, vol. 2, chapter 21 entitled
• Biochemical loop reactors In the event that the disperse phase has a higher density than the continuous phase, air can be introduced into the riser pipe in order to intensify the upwards movement of the liquid medium in said riser pipe.
• provision of the reaction system in the loop reactor with oxygen can be implemented with this embodiment of the method according to the invention.
• Loop reactors in which the method according to the invention is carried out may therefore be used as continuous extractors, fermenters and biological reactors using (bio)catalysts.
• Petroleum ether 40/60 having a density of 0.65 g/cm 3 was pumped in at the bottom of the riser pipe by means of the porous filter fitted in the bottom of the loop reactor and having a diameter of 25 mm (pore size ⁇ P4).
• the circulation in the loop reactor was made visible with alginate spherules suspended in the aqueous phase and having a diameter of 2 mm.
• the circulation time of said alginate spherules was approximately 3 seconds.
• An "internal" loop reactor according to Figure 3c having the dimensions mentioned in Example I was filled with petroleum ether 40/60. Water (9 g/dm 3 NaCl) was pumped into the internal pipe by means of the porous filter fitted in the top of the loop reactor and having a diameter of 25 mm (pore size ⁇ P4). The circulation in the loop reactor was made visible with alginate spherules having a diameter of 2 mm.
• Example III An "external" loop reactor according to Figure

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• Chemical & Material Sciences (AREA)
• Organic Chemistry (AREA)
• Life Sciences & Earth Sciences (AREA)
• Engineering & Computer Science (AREA)
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• Bioinformatics & Cheminformatics (AREA)
• Health & Medical Sciences (AREA)
• Chemical Kinetics & Catalysis (AREA)
• Microbiology (AREA)
• Biotechnology (AREA)
• Biochemistry (AREA)
• General Engineering & Computer Science (AREA)
• General Health & Medical Sciences (AREA)
• Genetics & Genomics (AREA)
• Biomedical Technology (AREA)
• Sustainable Development (AREA)
• General Chemical & Material Sciences (AREA)
• Mycology (AREA)
• Apparatus Associated With Microorganisms And Enzymes (AREA)

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• 1986
  • 1986-12-05 NL NL8603105A patent/NL8603105A/nl not_active Application Discontinuation
• 1987
  • 1987-12-04 WO PCT/NL1987/000035 patent/WO1988004317A1/en not_active Application Discontinuation
  • 1987-12-04 EP EP19880900251 patent/EP0292549A1/de not_active Withdrawn

Non-Patent Citations (1)

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