EP0223452A3 - Protection of plants against viral infection - Google Patents

Protection of plants against viral infection Download PDF

Info

Publication number
EP0223452A3
EP0223452A3 EP86308397A EP86308397A EP0223452A3 EP 0223452 A3 EP0223452 A3 EP 0223452A3 EP 86308397 A EP86308397 A EP 86308397A EP 86308397 A EP86308397 A EP 86308397A EP 0223452 A3 EP0223452 A3 EP 0223452A3
Authority
EP
European Patent Office
Prior art keywords
plant
plant cells
protection
virus
viral infection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP86308397A
Other versions
EP0223452B1 (en
EP0223452A2 (en
Inventor
Roger N. Beachy
Robert T. Fraley
Stephen G. Rogers
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Monsanto Co
Washington University in St Louis WUSTL
Original Assignee
Monsanto Co
Washington University in St Louis WUSTL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Co, Washington University in St Louis WUSTL filed Critical Monsanto Co
Publication of EP0223452A2 publication Critical patent/EP0223452A2/en
Publication of EP0223452A3 publication Critical patent/EP0223452A3/en
Application granted granted Critical
Publication of EP0223452B1 publication Critical patent/EP0223452B1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1131—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/12011—Geminiviridae
    • C12N2750/12022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/14011—Bromoviridae
    • C12N2770/14022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/40011—Tymoviridae
    • C12N2770/40022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Abstract

A process for producing genetically transformed plants which are resistant to infection by a plant virus, comprises the steps of: (a) inserting into the genome of a plant cell a recombinant, double-stranded DNA molecule comprising (i) a promoter which functions in plant cells to cause the production of RNA sequences of said plant virus; (ii) a DNA sequence that corresponds to an RNA sequence of said plant virus. (iii) a 3' non-translated region which functions in plant cells to cause the addition of polyadenylated nucleotides to the 3' end of said RNA sequence; (b) obtaining transformed plant cells; and (c) regenerating from the transformed plant cells genetically transformed plants which have increased resistance to infection by said plant virus. o
EP86308397A 1985-10-29 1986-10-29 Protection of plants against viral infection Expired - Lifetime EP0223452B1 (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US79238985A 1985-10-29 1985-10-29
US84491886A 1986-03-27 1986-03-27
US792389 1986-10-09
US06/917,027 US6608241B1 (en) 1985-10-29 1986-10-09 Protection of plants against viral infection
US844918 1986-10-09
US917027 1986-10-09

Publications (3)

Publication Number Publication Date
EP0223452A2 EP0223452A2 (en) 1987-05-27
EP0223452A3 true EP0223452A3 (en) 1989-08-30
EP0223452B1 EP0223452B1 (en) 1996-04-03

Family

ID=27419896

Family Applications (1)

Application Number Title Priority Date Filing Date
EP86308397A Expired - Lifetime EP0223452B1 (en) 1985-10-29 1986-10-29 Protection of plants against viral infection

Country Status (13)

Country Link
US (1) US6608241B1 (en)
EP (1) EP0223452B1 (en)
JP (4) JPH06339379A (en)
AT (1) ATE136330T1 (en)
AU (1) AU608204B2 (en)
BG (1) BG49720A3 (en)
CA (1) CA1341565C (en)
DE (1) DE3650507T2 (en)
DK (1) DK515186A (en)
ES (1) ES2087055T3 (en)
GR (1) GR3020438T3 (en)
IE (1) IE81098B1 (en)
IL (1) IL80433A (en)

Families Citing this family (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3650756T2 (en) * 1985-03-21 2001-10-04 Cornell Res Foundation Inc RESISTANCE DERIVED FROM PARASITE
GB8601680D0 (en) * 1986-01-23 1986-02-26 Agricultural Genetics Co Modification of plant viruses
US5175102A (en) * 1986-01-23 1992-12-29 Agricultural Genetics Company, Limited Modification of plant viruses or their effects
NZ219472A (en) * 1986-03-28 1990-08-28 Calgene Inc Regulation of phenotype in plant cells using dsdna constructs
US5107065A (en) * 1986-03-28 1992-04-21 Calgene, Inc. Anti-sense regulation of gene expression in plant cells
US5453566A (en) * 1986-03-28 1995-09-26 Calgene, Inc. Antisense regulation of gene expression in plant/cells
DE3751393T2 (en) * 1986-04-02 1996-02-22 Pioneer Hi Bred Int Plants resistant to the anti-sens RNA virus.
ATE119201T1 (en) * 1986-04-02 1995-03-15 Pioneer Hi Bred Int VIRUS RESISTANT PLANTS WITH A VIRAL COATING PROTEIN.
DE3850683T2 (en) * 1987-02-09 1994-10-27 Lubrizol Genetics Inc Hybrid RNA virus.
DE3865694D1 (en) * 1987-02-20 1991-11-28 Takeda Chemical Industries Ltd PLANTS RESISTANT TO "CUCUMBER MOSAIC VIRUS" INFECTION.
ATE205253T1 (en) * 1987-07-10 2001-09-15 Syngenta Participations Ag INDUCABLE VIRUS RESISTANCE IN PLANTS
US5623066A (en) * 1987-12-21 1997-04-22 Asgrow Seed Company Cucumber mosaic virus coat protein gene
AU2927689A (en) * 1987-12-21 1989-07-19 Seminis Vegetable Seeds, Inc. Cucumber mosaic virus coat protein gene
FR2631973B1 (en) * 1988-05-30 1992-12-11 Agronomique Inst Nat Rech COMPLETE NUCLEOTIDE SEQUENCE OF COMPLEMENTARY DNA OF POTYVIRUS GENOMIC RNA, GENES ENCODING POTYVIRUS CAPSIDE PROTEIN AND APPLICATIONS OF THESE GENES TO CREATE POTYVIRUS-RESISTANT TRANSGENIC PLANTS
EP0429483B1 (en) * 1988-08-19 1997-11-12 Seminis Vegetable Seeds, Inc. Potyvirus coat protein genes and plants transformed therewith
WO1990002185A1 (en) * 1988-08-19 1990-03-08 The Upjohn Company Cocumber mosaic virus coat protein gene
GB8827094D0 (en) * 1988-11-19 1988-12-21 Scottish Crop Research Inst Dna sequence encoding coat protein gene of potato leafroll virus
DK0413019T3 (en) 1989-02-24 2001-11-12 Monsanto Technology Llc Synthetic plant genes and methods for their production
US5428144A (en) * 1989-04-07 1995-06-27 Pioneer Hi-Bred International, Inc. Maize dwarf mosaic virus cDNA
ATE114720T1 (en) * 1989-09-20 1994-12-15 Upjohn Co SOMATIC EMBRYOGENESIS OF PUMPKINS.
US5939600A (en) * 1989-11-03 1999-08-17 Goldbach; Robert Willem Nucleic acids encoding tospovirus genome and expression thereof
HUT57265A (en) * 1989-11-03 1991-11-28 Zaadunie Bv Process for producing plants of diminished infection-sensitivity
JPH04144685A (en) * 1989-11-28 1992-05-19 Nippon Oil Co Ltd Cdna of orsv gene
FR2658988A1 (en) * 1990-03-02 1991-09-06 Biosem Rhizomania-resistant beet
DK0517833T4 (en) * 1990-03-02 2008-11-17 Bayer Bioscience Nv Genetic transformation and regeneration of sugar beet
US5514570A (en) * 1991-08-27 1996-05-07 Cornell Research Foundation Squash mosaic virus genes and plants transformed therewith
WO1993014210A1 (en) * 1992-01-08 1993-07-22 Sandoz Ltd. Virus resistant corn plants
US5569828A (en) * 1993-03-24 1996-10-29 Pioneer Hi-Bred International, Inc. Maize chlorotic dwarf virus and resistance thereto
CA2166786A1 (en) * 1993-07-09 1995-01-19 Maury L. Boeshore Lettuce infectious yellows virus genes
GB9505907D0 (en) * 1995-03-23 1995-05-10 Sandoz Ltd Improvements in or relating to organic compounds
HUP9904168A3 (en) * 1996-11-04 2002-07-29 Univ Kentucky Coat protein mediated resistance in plants
GB9703146D0 (en) 1997-02-14 1997-04-02 Innes John Centre Innov Ltd Methods and means for gene silencing in transgenic plants
GB9827152D0 (en) 1998-07-03 1999-02-03 Devgen Nv Characterisation of gene function using double stranded rna inhibition
JP4446085B2 (en) 2001-10-05 2010-04-07 独立行政法人産業技術総合研究所 Plant introduced with phenol oxidase gene, and method for producing phenol oxidase by said plant
EP1718663B1 (en) 2004-01-20 2011-07-13 Monsanto Technology, LLC Chimeric promoters for use in plants
WO2006047495A2 (en) 2004-10-21 2006-05-04 Venganza Inc Methods and materials for conferring resistance to pests and pathogens of plants
US7838729B2 (en) 2007-02-26 2010-11-23 Monsanto Technology Llc Chloroplast transit peptides for efficient targeting of DMO and uses thereof
US8580942B2 (en) 2008-04-07 2013-11-12 Monsanto Technology Llc Plant regulatory elements from a metallothionein-like gene and uses thereof
WO2010099431A2 (en) 2009-02-27 2010-09-02 Monsanto Technology Llc Hydroponic apparatus and methods of use
US20100257634A1 (en) * 2009-04-03 2010-10-07 Venganza Inc. Bioassay for gene silencing constructs
US8937214B2 (en) 2009-10-23 2015-01-20 Monsanto Technology Llc Methods and compositions for expression of transgenes in plants
ES2882425T3 (en) 2010-01-14 2021-12-01 Monsanto Technology Llc Regulatory elements of plants and their uses
JP6039560B2 (en) 2010-08-30 2016-12-07 ダウ アグロサイエンシィズ エルエルシー Sugarcane-boiled virus (SCBV) enhancer and its use in plant functional genomics
ES2666149T3 (en) 2011-03-25 2018-05-03 Monsanto Technology Llc Regulatory elements of plants and their uses
CA3064703C (en) 2011-05-13 2023-01-31 Monsanto Technology Llc Plant regulatory elements and uses thereof
US10077450B2 (en) 2012-02-29 2018-09-18 Dow Agrosciences Llc Sugarcane bacilliform viral (SCBV) enhancer and its use in plant functional genomics
US9663793B2 (en) 2012-04-20 2017-05-30 Monsanto Technology, Llc Plant regulatory elements and uses thereof
JP6457399B2 (en) 2012-12-19 2019-01-23 モンサント テクノロジー エルエルシー Plant regulatory elements and uses thereof
CR20200120A (en) 2013-03-14 2020-06-05 Monsanto Technology Llc Plant regulatory elements and uses thereof
CA3145660A1 (en) 2013-03-14 2014-10-02 Monsanto Technology Llc Plant regulatory elements and uses thereof
US11519011B2 (en) 2015-03-26 2022-12-06 The Texas A&M University System Conversion of lignin into bioplastics and lipid fuels
EA039403B1 (en) 2016-03-11 2022-01-24 Монсанто Текнолоджи Ллс Plant regulatory elements and uses thereof
US10351866B2 (en) 2016-05-24 2019-07-16 Monsanto Technology Llc Plant regulatory elements and uses thereof
CA3029271A1 (en) 2016-06-28 2018-01-04 Cellectis Altering expression of gene products in plants through targeted insertion of nucleic acid sequences
US11236355B2 (en) * 2017-08-22 2022-02-01 The Regents Of The University Of California ROQ1 provides resistance to both xanthomonas and pseudomonas in plants
EP3737747A1 (en) 2018-01-12 2020-11-18 The Texas A&M University System Increasing plant bioproduct yield
AR125217A1 (en) 2021-03-26 2023-06-28 Flagship Pioneering Innovations Vii Llc PRODUCTION OF CIRCULAR POLYRIBONUCLEOTIDES IN A PROKARYOTA SYSTEM
TW202300650A (en) 2021-03-26 2023-01-01 美商旗艦先鋒創新有限責任（Ｖｉｉ）公司 Production of circular polyribonucleotides in a eukaryotic system
WO2022204466A1 (en) 2021-03-26 2022-09-29 Flagship Pioneering Innovations Vii, Llc Production of circular polyribonucleotides in a prokaryotic system
WO2023077118A1 (en) 2021-11-01 2023-05-04 Flagship Pioneering Innovations Vii, Llc Polynucleotides for modifying organisms
WO2023141540A2 (en) 2022-01-20 2023-07-27 Flagship Pioneering Innovations Vii, Llc Polynucleotides for modifying organisms
WO2024052856A1 (en) 2022-09-09 2024-03-14 Friedrich Alexander Universität Erlangen-Nürnberg Plant regulatory elements and uses thereof

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1985004898A1 (en) * 1984-04-26 1985-11-07 The Australian National University An improved method of cloning double-stranded rna, and its use in the production of viral gene clones
WO1986005516A1 (en) * 1985-03-21 1986-09-25 Duke University Parasite-derived resistance
EP0223399A1 (en) * 1985-10-16 1987-05-27 Agracetus, Inc. Effecting somatic changes in plants through the use of negative strand RNAS
EP0240332A2 (en) * 1986-04-02 1987-10-07 Pioneer Hi-Bred International, Inc. Virus resistant plants having antisense RNA
EP0240331A2 (en) * 1986-04-02 1987-10-07 Pioneer Hi-Bred International, Inc. Virus resistant plants having a viral coat protein
EP0242016A1 (en) * 1986-01-23 1987-10-21 Agricultural Genetics Company Limited Modification of plant viruses or their effects

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4407956A (en) * 1981-03-13 1983-10-04 The Regents Of The University Of California Cloned cauliflower mosaic virus DNA as a plant vehicle
US4527287A (en) 1983-06-24 1985-07-09 Figgie International Inc. Baseball glove or mitt
US5580716A (en) 1985-03-21 1996-12-03 Stephen A. Johnston Parasite-derived resistance
KR950012900B1 (en) * 1992-08-19 1995-10-23 주식회사진로 Expression vector for anti-virus protein gene

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1985004898A1 (en) * 1984-04-26 1985-11-07 The Australian National University An improved method of cloning double-stranded rna, and its use in the production of viral gene clones
WO1986005516A1 (en) * 1985-03-21 1986-09-25 Duke University Parasite-derived resistance
EP0223399A1 (en) * 1985-10-16 1987-05-27 Agracetus, Inc. Effecting somatic changes in plants through the use of negative strand RNAS
EP0242016A1 (en) * 1986-01-23 1987-10-21 Agricultural Genetics Company Limited Modification of plant viruses or their effects
EP0240332A2 (en) * 1986-04-02 1987-10-07 Pioneer Hi-Bred International, Inc. Virus resistant plants having antisense RNA
EP0240331A2 (en) * 1986-04-02 1987-10-07 Pioneer Hi-Bred International, Inc. Virus resistant plants having a viral coat protein

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BIOTECHNOLOGY, vol. 4, February 1986, page 96; H. BIALY et al.: "A new route to virus resistance in plants" *
CHEMICAL ABSTRACTS, vol. 106, no. 1, January 1987, page 134, ref.no. 1337j; Columbus, Ohio, US; R.N. BEACHY et al.: "Potential for applying genetic transformation to studies of viral pathogenesis and cross-protection", & BIOTECHNOL. PLANT SCI.: RELEVANCE AGRIC. EIGHTIES, (Symp.) 1985, 265-75 *
HORTSCIENCE, vol. 20, no. 5, October 1985, pages 848-852; R.I. HAMILTON: "Using plant viruses for disease control" *
JOURNAL OF CELL. BIOCHEM., vol. 0, no. 10, part C, page 41, ref.no. J 108; L.S. LOSCH-FRIES et al.: "Cloning of alfalfa mosaic virus caot protein gene and anti-sense RNA into a binary vector and their expression in transformed tobacco tissue" *
SCIENCE, vol. 232, May 9, 1986, pages 738-743; P.P. ABEL et al.: "Delay of disease development in transgenic plants that express the tabacco mosaic virus coat protein gene" *
THE EMBO JOURNAL, vol. 4, no. 8, 1985, pages 1921-1926; IRL Press Ltd., Oxford GB M.W. BEVAN et al.: "Expression of tobacco mosaic virus coat protein by a cauliflower mosaic virus promoter in plants transformed by Agrobacterium" *

Also Published As

Publication number Publication date
CA1341565C (en) 2007-12-04
DK515186A (en) 1987-04-30
GR3020438T3 (en) 1996-10-31
ATE136330T1 (en) 1996-04-15
IE81098B1 (en) 2000-03-08
JP2000157288A (en) 2000-06-13
DK515186D0 (en) 1986-10-28
JP2000041512A (en) 2000-02-15
IL80433A (en) 1996-11-14
BG49720A3 (en) 1992-01-15
DE3650507T2 (en) 1996-10-17
EP0223452B1 (en) 1996-04-03
AU608204B2 (en) 1991-03-28
EP0223452A2 (en) 1987-05-27
IE862828L (en) 1987-04-29
DE3650507D1 (en) 1996-05-09
US6608241B1 (en) 2003-08-19
JPH06339379A (en) 1994-12-13
AU6452886A (en) 1987-04-30
ES2087055T3 (en) 1996-07-16
JPH0851986A (en) 1996-02-27
JP3281512B2 (en) 2002-05-13

Similar Documents

Publication Publication Date Title
EP0223452A3 (en) Protection of plants against viral infection
Gebhard et al. A new family of interspersed repetitive DNA sequences in the mouse genome
Pays et al. Trypanosoma brucei: posttranscriptional control of the variable surface glycoprotein gene expression site
EP0331939A3 (en) Method and compositions for conferring resistance to retroviral infection
PL333070A1 (en) Infectious clones of rna viruses and vaccines based on them
DE69020151D1 (en) GENETICALLY SHAPED NEW PLANT PHENOTYPES.
HU9303166D0 (en) Hcv genomic sequences for diagnostics and therapeutics
NO874750L (en) Certain DNA sequences derived from a papilloma virus genome, their use in in vitro diagnostics and production of antigenic preparations.
EP0240332A3 (en) Virus resistant plants having antisense rna
Re et al. Genomic and copy-back 3'termini in Sendai virus defective interfering RNA species
CA2095109A1 (en) Process for production of exogenous gene or its product in plant cells
Berry-Lowe The chloroplast glutamate tRNA gene required for δ-aminolevulinate synthesis
DE59109072D1 (en) Cloning of N-methylhydantoinase
GR3015892T3 (en) Virus resistant plants having a viral coat protein.
ATE370239T1 (en) METHOD FOR PROVIDING BNYVV RESISTANCE IN SUGAR BEET PLANTS
FR2651007B1 (en) NUCLEIC ACID FRAGMENTS AND DIAGNOSTIC REAGENTS DERIVED FROM THE HHV6 / SIE GENOME AND METHOD FOR DIAGNOSING HHV6 INFECTIONS
ES2125842A1 (en) INFECTIOUS VECTORS AND CLONES OF PLANTS DERIVED FROM THE TURNIP MOSAIC VIRUS (TuMV)
HUP0102517A2 (en) Genomic sequences upstream of the coding region of the ifn-alpha2 gene for protein production and delivery
MORINAGA et al. Molecular cloning of full-length of bean golden mosaic virus DNA
Smith et al. Chemistry of Plant Viruses
TH8089B (en) Plants with reduced susceptibility to infection with toospovirus, the genetic material used has led to this acceptance.
TH10000A (en) Plants that are less susceptible to infection with toospovirus, the genetic material used has led to this acceptance.

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

AK Designated contracting states

Kind code of ref document: A2

Designated state(s): AT BE CH DE ES FR GB GR IT LI LU NL SE

PUAL Search report despatched

Free format text: ORIGINAL CODE: 0009013

AK Designated contracting states

Kind code of ref document: A3

Designated state(s): AT BE CH DE ES FR GB GR IT LI LU NL SE

17P Request for examination filed

Effective date: 19900130

17Q First examination report despatched

Effective date: 19910508

GRAH Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOS IGRA

GRAA (expected) grant

Free format text: ORIGINAL CODE: 0009210

AK Designated contracting states

Kind code of ref document: B1

Designated state(s): AT BE CH DE ES FR GB GR IT LI LU NL SE

REF Corresponds to:

Ref document number: 136330

Country of ref document: AT

Date of ref document: 19960415

Kind code of ref document: T

REF Corresponds to:

Ref document number: 3650507

Country of ref document: DE

Date of ref document: 19960509

ITF It: translation for a ep patent filed

Owner name: BARZANO' E ZANARDO ROMA S.P.A.

REG Reference to a national code

Ref country code: ES

Ref legal event code: BA2A

Ref document number: 2087055

Country of ref document: ES

Kind code of ref document: T3

REG Reference to a national code

Ref country code: CH

Ref legal event code: NV

Representative=s name: KATZAROV S.A.

REG Reference to a national code

Ref country code: ES

Ref legal event code: FG2A

Ref document number: 2087055

Country of ref document: ES

Kind code of ref document: T3

ET Fr: translation filed
REG Reference to a national code

Ref country code: GR

Ref legal event code: FG4A

Free format text: 3020438

PLBE No opposition filed within time limit

Free format text: ORIGINAL CODE: 0009261

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT

26N No opposition filed
REG Reference to a national code

Ref country code: GB

Ref legal event code: IF02

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: AT

Payment date: 20021003

Year of fee payment: 17

Ref country code: SE

Payment date: 20021003

Year of fee payment: 17

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: CH

Payment date: 20021004

Year of fee payment: 17

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: LU

Payment date: 20021017

Year of fee payment: 17

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: GR

Payment date: 20021024

Year of fee payment: 17

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: AT

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20031029

Ref country code: LU

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20031029

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20031030

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: LI

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20031031

Ref country code: CH

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20031031

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: GR

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20040504

EUG Se: european patent has lapsed
REG Reference to a national code

Ref country code: CH

Ref legal event code: PL

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: NL

Payment date: 20050929

Year of fee payment: 20

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: FR

Payment date: 20051017

Year of fee payment: 20

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: GB

Payment date: 20051026

Year of fee payment: 20

Ref country code: ES

Payment date: 20051026

Year of fee payment: 20

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: IT

Payment date: 20051028

Year of fee payment: 20

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: BE

Payment date: 20051122

Year of fee payment: 20

REG Reference to a national code

Ref country code: GB

Ref legal event code: PE20

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: DE

Payment date: 20051130

Year of fee payment: 20

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: GB

Free format text: LAPSE BECAUSE OF EXPIRATION OF PROTECTION

Effective date: 20061028

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: NL

Free format text: LAPSE BECAUSE OF EXPIRATION OF PROTECTION

Effective date: 20061029

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: ES

Free format text: LAPSE BECAUSE OF EXPIRATION OF PROTECTION

Effective date: 20061030

NLV7 Nl: ceased due to reaching the maximum lifetime of a patent

Effective date: 20061029

REG Reference to a national code

Ref country code: ES

Ref legal event code: FD2A

Effective date: 20061030

BE20 Be: patent expired

Owner name: *MONSANTO CY

Effective date: 20061029

Owner name: *WASHINGTON UNIVERSITY

Effective date: 20061029