TH10000A - Plants that are less susceptible to infection with toospovirus, the genetic material used has led to this acceptance. - Google Patents

Abstract

The invention involved plants with reduced susceptibility to toospovirus infection, the genetic material used to create this tolerance, a probe for isolation or diagnosis. And the processes for obtaining those plants, genetic material and probes.

Claims (3)

1. Recombinant DNA structure that contains a DNA sequence for transcription a) a tostovirus RNA sequence or a homologous RNA sequence b) a given RNA sequence a ) That defines the code of the toospovirus protein In which one or more of the Codons are replaced With the synonyms of those codons (That is, the codons corresponding to the same amino acid or signal stop), or part of that sequence, or the homologous RNA sequence to those sequences, or C). The matching RNA fits the homologous RNA sequence. Gus and those sequences or where the DNA is under control of the promoter genotype. And functional terminators in plants 2. DNA structure according to claim 1, where the DNA sequence defines the transcription of i) nucleotide sequences 1 to 2915 of S RNA ii) 89 to 1483 nucleotide sequence of S RNA iii) hairpin part of S RNA iv)? Pan handle? Of S RNA v) 2763 to 1987 nucleotide sequence of S RNA vi) 4462 to 1 nucleotide sequence of L RNA vii) 41 to 2 of L nucleotide sequence RNA viii) 3980 to 46 nucleotide sequence of L RNA ix)? Of L RNA x) RNA sequence that matches the 1987 to 2763 nucleotide sequence of S RNA xi), the matched RNA sequence fits the 89th to 1483 nucleotide sequence of S RNA xii. ) RNA sequence matching to the 1st to 4462 nucleotide sequence of L RNA xiii) RNA sequence matching to the 2nd to 41th nucleotide sequence of L RNA xiv) RNA sequence The nucleotide sequence 46 to 3980 of the L RNA xv), the 89th to 1483 nucleotide sequence of S RNA, which replaces the code one. Or even more (Xvi) the 2763 to 1987 nucleotide sequence of S RNA in which the codon one is replaced. (Xvii) 3980 to 236 nucleotide sequences of L RNA in which the codon is replaced. (Xviii) 4462 to 236 nucleotide sequences of L RNA in which the codon is replaced. Or more, with those codon synodes (xix) the homologous RNA sequence with the nucleotide sequence defined under i) to xiv) in the origin of this document xx) Fragment The DNA sequence defined under i) to xix) at the top of this document 3. The DNA structure of claim 1, coded as the toospovirus? RNA of the pan side. Or it comes out as a homologous RNA sequence with those sequences. 4. The DNA structure of claim 1 that determines the code to be the tosto virus sequence. ? RNA of the filter reads open in WIRAL COMPLIANCE RESCENSE. Or come out as a matching RNA sequence in which one codon is replaced. Or even more with those codons synonyms. 5. The DNA structure of claim 1 that is coded to allow the transcription of the toospovirus sequence? RNA of the hairpin section. Or come out as a homologous RNA sequence with that sequence 6. DNA structure of one of the claims 1 to 5, which defines the code. RNA or totospovirus sequence, which is replaced by the codon one. Or more with the synonyms of those condos Or to come out as a homologous RNA sequence with at least 20 nucleotides of those sequences 7. The DNA structure of claim 6 that defines the cipher to achieve the toospo sequence. Viruses? RNA, or is issued as a sequence of tostovirus RNA which is substituted for the codon. Or even more with those codon synonyms. Or is it a homologous RNA sequence with those sequences like 50 nucleotides 8. DNA structure of claim 1 where the DNA sequence is coded. In order to exfoliate the code in the sequence 5? Together with the final sequence 3? Of the S RNA or the L RNA of the virus, respectively 9. DNA structure of any 1 to 8 claim The promoter is a promoter derived from viruses, fungi, bacteria, animals or plants that act in plant cells 1 0. DNA structure of claim 9, in which the terminator is the terminator derived from Viruses, fungi, bacteria, animals or plants that are active in plant cells 1 1. a probe as it contains Where an oligonucleotide sequence or a complementary pairing with a toospovirus S RNA sequence or an L RNA nucleotide sequence of a toospovirus, or Fragments of these sequences containing at least 15 nucleotides 1. 2.Probe, as defined in claim 11, in which the oligonucleotide sequence contains 400 to 600 nucleotides 1. 3. The process of preparing plants with the DNA structure as defined in one of the claims 1 to 10 are constituents in the genome of plants. It consists of a) insertion of the DNA structure of claim 1 into the genome of the plant cell; b) obtaining the genetically altered cell; The genetic material has been changed.