EP0200417A3 - Production of protein - Google Patents

Production of protein Download PDF

Info

Publication number
EP0200417A3
EP0200417A3 EP86302833A EP86302833A EP0200417A3 EP 0200417 A3 EP0200417 A3 EP 0200417A3 EP 86302833 A EP86302833 A EP 86302833A EP 86302833 A EP86302833 A EP 86302833A EP 0200417 A3 EP0200417 A3 EP 0200417A3
Authority
EP
European Patent Office
Prior art keywords
protein
escherichia coli
ion source
start codon
present
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP86302833A
Other versions
EP0200417A2 (en
EP0200417B1 (en
Inventor
Kazuaki Kitano
Shigeru Fujimoto
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Takeda Pharmaceutical Co Ltd
Original Assignee
Takeda Chemical Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/JP1985/000248 external-priority patent/WO1986006410A1/en
Priority claimed from JP61045667A external-priority patent/JPH0634746B2/en
Application filed by Takeda Chemical Industries Ltd filed Critical Takeda Chemical Industries Ltd
Priority to AT86302833T priority Critical patent/ATE81675T1/en
Publication of EP0200417A2 publication Critical patent/EP0200417A2/en
Publication of EP0200417A3 publication Critical patent/EP0200417A3/en
Application granted granted Critical
Publication of EP0200417B1 publication Critical patent/EP0200417B1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Abstract

The present invention provides an improvement in a method for producing a protein by cultivating Escherichia coli having an expression vector which contains a structural gene for the protein downstream from the translational start codon, which comprises cultivating the Escherichia coli in a medium containing (1) an iron ion source, manganese ion source or a mixture thereof and (2) a nitrogen source from a natural origin to increase the yield of the protein free of methionine corresponding to the translational starting codon ATG at the N-terminus. According to the method of the present invention, protein free of the N-terminal methionine residue corresponding to the translation start codon ATG can be obtained in an increased yield.
EP86302833A 1985-04-30 1986-04-16 Production of protein Expired - Lifetime EP0200417B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AT86302833T ATE81675T1 (en) 1985-04-30 1986-04-16 PRODUCTION OF A PROTEIN.

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
PCT/JP1985/000248 WO1986006410A1 (en) 1985-04-30 1985-04-30 Process for increasing yield of interleukin-2
WOPCT/JP85/00248 1985-04-30
JP221517/85 1985-10-03
JP22151785 1985-10-03
JP61045667A JPH0634746B2 (en) 1985-04-30 1986-03-03 Interleukin Revenue Increase Method
JP45667/86 1986-03-03

Publications (3)

Publication Number Publication Date
EP0200417A2 EP0200417A2 (en) 1986-11-05
EP0200417A3 true EP0200417A3 (en) 1988-08-31
EP0200417B1 EP0200417B1 (en) 1992-10-21

Family

ID=27292335

Family Applications (1)

Application Number Title Priority Date Filing Date
EP86302833A Expired - Lifetime EP0200417B1 (en) 1985-04-30 1986-04-16 Production of protein

Country Status (10)

Country Link
EP (1) EP0200417B1 (en)
CN (1) CN1012645B (en)
AT (1) ATE81675T1 (en)
AU (1) AU603545B2 (en)
CA (1) CA1273591A (en)
DE (1) DE3686981T2 (en)
DK (1) DK196086A (en)
IE (1) IE58766B1 (en)
IL (1) IL78543A (en)
NZ (1) NZ215994A (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3532134A1 (en) * 1985-09-10 1987-03-12 Basf Ag METHOD FOR PRODUCING PROTEINS OF SPECIFIC N-TERMINAL STRUCTURE WITH THE AID OF PROKARYONTS
EP0315950A3 (en) * 1987-11-09 1990-05-23 Otsuka Pharmaceutical Co., Ltd. Method of incubating cells expressing a recombinant protein
CN1062311C (en) * 1996-12-27 2001-02-21 中国医学科学院基础医学研究所 Fused gene of IL-2 or its derivative and ANP or its derivative, three kinds of protein product thereof and their application, and vector containing said gene and construction method thereof
DE19749259A1 (en) * 1997-11-07 1999-05-12 Dade Behring Marburg Gmbh Process for the production of functional recombinant tissue factor
CN102643826A (en) * 2012-04-10 2012-08-22 上海交通大学 Thymosin extrasin beta4 quadruplet gene and expression method thereof in Escherichia coli

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0076489A2 (en) * 1981-10-03 1983-04-13 Ciba-Geigy Ag Deoxyribonucleic acids, recombinant deoxyribonucleic acids, hosts containing them, polypeptides and process for their production
EP0144064A2 (en) * 1983-11-30 1985-06-12 Takeda Chemical Industries, Ltd. Method for producing interferons
EP0146354A2 (en) * 1983-12-16 1985-06-26 Genentech, Inc. Recombinant gamma interferons and pharmaceutical compositions containing them
EP0156355A2 (en) * 1984-03-28 1985-10-02 Cetus Oncology Corporation Method of improving the yield of heterologous protein produced by cultivating recombinant bacteria
EP0214564A2 (en) * 1985-09-10 1987-03-18 BASF Aktiengesellschaft Process for the preparation of proteins with a determined N-terminal structure with the aid of procaryotes

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0076489A2 (en) * 1981-10-03 1983-04-13 Ciba-Geigy Ag Deoxyribonucleic acids, recombinant deoxyribonucleic acids, hosts containing them, polypeptides and process for their production
EP0144064A2 (en) * 1983-11-30 1985-06-12 Takeda Chemical Industries, Ltd. Method for producing interferons
EP0146354A2 (en) * 1983-12-16 1985-06-26 Genentech, Inc. Recombinant gamma interferons and pharmaceutical compositions containing them
EP0156355A2 (en) * 1984-03-28 1985-10-02 Cetus Oncology Corporation Method of improving the yield of heterologous protein produced by cultivating recombinant bacteria
EP0214564A2 (en) * 1985-09-10 1987-03-18 BASF Aktiengesellschaft Process for the preparation of proteins with a determined N-terminal structure with the aid of procaryotes

Also Published As

Publication number Publication date
DE3686981D1 (en) 1992-11-26
DK196086A (en) 1986-10-31
IE860997L (en) 1986-10-30
AU5652286A (en) 1986-11-06
IL78543A0 (en) 1986-08-31
IE58766B1 (en) 1993-11-03
CN86102977A (en) 1986-10-29
AU603545B2 (en) 1990-11-22
CN1012645B (en) 1991-05-22
CA1273591A (en) 1990-09-04
DE3686981T2 (en) 1993-07-08
DK196086D0 (en) 1986-04-29
ATE81675T1 (en) 1992-11-15
IL78543A (en) 1994-11-28
EP0200417A2 (en) 1986-11-05
NZ215994A (en) 1989-07-27
EP0200417B1 (en) 1992-10-21

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