EP0181213B1 - Céphalosporium acrémonium transformé avec un déterminant de résistance aminoglycosidique - Google Patents

Céphalosporium acrémonium transformé avec un déterminant de résistance aminoglycosidique Download PDF

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Publication number
EP0181213B1
EP0181213B1 EP85308099A EP85308099A EP0181213B1 EP 0181213 B1 EP0181213 B1 EP 0181213B1 EP 85308099 A EP85308099 A EP 85308099A EP 85308099 A EP85308099 A EP 85308099A EP 0181213 B1 EP0181213 B1 EP 0181213B1
Authority
EP
European Patent Office
Prior art keywords
gene
cephalosporium acremonium
aminoglycoside
plasmid
resistance
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
EP85308099A
Other languages
German (de)
English (en)
Other versions
EP0181213A2 (fr
EP0181213A3 (en
Inventor
A. Migual Penalva
Angeles Tourino
Cristina Patino
Florentina Sanchez
Victor Rubio
Jose Maria Fernandez-Sousa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Antibioticos SA
Original Assignee
Antibioticos SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB848428094A external-priority patent/GB8428094D0/en
Priority claimed from GB858503441A external-priority patent/GB8503441D0/en
Priority claimed from GB858508353A external-priority patent/GB8508353D0/en
Application filed by Antibioticos SA filed Critical Antibioticos SA
Priority to AT85308099T priority Critical patent/ATE56753T1/de
Publication of EP0181213A2 publication Critical patent/EP0181213A2/fr
Publication of EP0181213A3 publication Critical patent/EP0181213A3/en
Application granted granted Critical
Publication of EP0181213B1 publication Critical patent/EP0181213B1/fr
Expired legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/925—Cephalosporium
    • Y10S435/926—Cephalosporium acremonium

Claims (9)

1. Cephalosporium acremonium transformé avec un vecteur comprenant un marqueur de résistance aminoglycosidique, qui confère la résistance au G418.
2. Cephalosporium acremonium selon la revendication 1, dans lequel ladite résistance est fournie par un gène codant pour une aminoglycoside 3'-phosphotransférase.
3. Cephalosporium acremonium selon la revendication 2, dans lequel ledit gène est séletionné dans le génome d'E. Coli contenant le tranposon Tn601(903), le transposon Tn5 ou dans le génome de Streptomyces fradiae.
4. Cephalosporium acremonium selon la revendication 2 ou la revendication 3, dans lequel la transcription dudit gène est activée par un promoteur eucaryotique.
5. Cephalosporium acremonium selon la revendication 4, dans lequel ledit promoteur eucaryotique comprend le promoteur du gène Saccharomyces cerevisiae alcool déhydrogenase 1 (ADCI).
6. Vecteur capable de transformer Cephalosporium acremonium avec le gène de résistance aminoglycosidique G418.
7. Plasmide pMA4 ou équivalent fonctionnel de celui-ci, déposé dans E. Coli au "National Collection of Industrial and Marine Bacteria" le 1er Novembre 1984 sous le numéro d'accession NCIB 12025.
8. Vecteur capable de transformer Cephalosporium acremonium à résistance aminoglycosidique, ledit vecteur incluant un promoteur eucaryotique et un gène non-eucaryotique codant pour la résistance G418 aminoglycosidique, ledit gène non-eucaryotique étant transcriptable à partir dudit promoteur.
9. Procédé de sélection de Cephalosporium acremonium transformé selon la revendication 1 des cellules de Cephalosporium acremonium non transformées comprenant le traitement des cellules avec l'aminoglycoside G418.
EP85308099A 1984-11-07 1985-11-07 Céphalosporium acrémonium transformé avec un déterminant de résistance aminoglycosidique Expired EP0181213B1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AT85308099T ATE56753T1 (de) 1984-11-07 1985-11-07 Cephalosporium acremonium,welches mit einem aminoglycosidresistenz transformiert ist.

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
GB8428094 1984-11-07
GB848428094A GB8428094D0 (en) 1984-11-07 1984-11-07 Cephalosporium acremonium
GB858503441A GB8503441D0 (en) 1985-02-11 1985-02-11 Cephalosporium acremonium
GB8503441 1985-02-11
GB8508353 1985-03-29
GB858508353A GB8508353D0 (en) 1985-03-29 1985-03-29 Cephalosporium acremonium

Publications (3)

Publication Number Publication Date
EP0181213A2 EP0181213A2 (fr) 1986-05-14
EP0181213A3 EP0181213A3 (en) 1986-10-29
EP0181213B1 true EP0181213B1 (fr) 1990-09-19

Family

ID=27262507

Family Applications (1)

Application Number Title Priority Date Filing Date
EP85308099A Expired EP0181213B1 (fr) 1984-11-07 1985-11-07 Céphalosporium acrémonium transformé avec un déterminant de résistance aminoglycosidique

Country Status (4)

Country Link
US (1) US4753877A (fr)
EP (1) EP0181213B1 (fr)
DE (1) DE3579788D1 (fr)
ES (1) ES8605580A1 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8508800D0 (en) * 1985-04-04 1985-05-09 Glaxo Group Ltd Microbiological process
AU606033B2 (en) * 1985-04-15 1991-01-31 Dsm Ip Assets B.V. Promotors of filamentous fungi and use thereof
US5198345A (en) * 1985-04-15 1993-03-30 Gist-Brocades N.V. Vectors in use in filamentous fungi
US20010049612A1 (en) * 2000-05-10 2001-12-06 Corporate Compensation Plans, Inc. Survivor's benefit plan

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3121815A1 (de) * 1981-06-02 1983-02-24 Hoechst Ag, 6000 Frankfurt Hybridvektor, verfahren zu seiner herstellung und seine verwendung
IL66065A (en) * 1981-06-22 1989-06-30 Lilly Co Eli Recombinant dna cloning vectors and the e.coli transformants thereof
DE3141691A1 (de) * 1981-10-21 1983-05-19 Hoechst Ag, 6230 Frankfurt Plasmid pac 1, verfahren zu seiner gewinnung und seine verwendung
CA1207687A (fr) * 1982-07-07 1986-07-15 Alan M. Lambowitz Element extrachromosomique fonctionnel pouvant se repliquer dans des fungi filamenteux
JPS59135895A (ja) * 1982-10-04 1984-08-04 M S C:Kk 抗生物質の生産を促進する機能を有する化合物
US4762786A (en) * 1984-09-27 1988-08-09 Eli Lilly And Company Vectors and conditions which allow genetic transformation of cephalosporium

Also Published As

Publication number Publication date
ES8605580A1 (es) 1986-03-16
EP0181213A2 (fr) 1986-05-14
ES548663A0 (es) 1986-03-16
DE3579788D1 (de) 1990-10-25
US4753877A (en) 1988-06-28
EP0181213A3 (en) 1986-10-29

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