EP0161796A1 - Process for producing N-acylphenylalanines - Google Patents

Process for producing N-acylphenylalanines Download PDF

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EP0161796A1
EP0161796A1 EP85302503A EP85302503A EP0161796A1 EP 0161796 A1 EP0161796 A1 EP 0161796A1 EP 85302503 A EP85302503 A EP 85302503A EP 85302503 A EP85302503 A EP 85302503A EP 0161796 A1 EP0161796 A1 EP 0161796A1
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substituted
catalyst
oxazolone
group
palladium
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EP0161796B1 (en
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Ryuichi Mita
Toshio Katoh
Chojiro Higuchi
Akihiro Yamaguchi
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Mitsui Toatsu Chemicals Inc
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Mitsui Toatsu Chemicals Inc
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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07CACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C231/00Preparation of carboxylic acid amides
    • C07C231/10Preparation of carboxylic acid amides from compounds not provided for in groups C07C231/02 - C07C231/08
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07CACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C233/00Carboxylic acid amides
    • C07C233/01Carboxylic acid amides having carbon atoms of carboxamide groups bound to hydrogen atoms or to acyclic carbon atoms
    • C07C233/45Carboxylic acid amides having carbon atoms of carboxamide groups bound to hydrogen atoms or to acyclic carbon atoms having the nitrogen atom of at least one of the carboxamide groups bound to a carbon atom of a hydrocarbon radical substituted by carboxyl groups
    • C07C233/46Carboxylic acid amides having carbon atoms of carboxamide groups bound to hydrogen atoms or to acyclic carbon atoms having the nitrogen atom of at least one of the carboxamide groups bound to a carbon atom of a hydrocarbon radical substituted by carboxyl groups with the substituted hydrocarbon radical bound to the nitrogen atom of the carboxamide group by an acyclic carbon atom

Definitions

  • This invention relates to a process for producing substituted or unsubstituted N-acylphenylalanines.
  • N-acylphenylalanines are important compounds as precursors of phenylalanines.
  • unsubstituted N-acylphenylalanines are categorized as essential amino acids and are important compounds as precursors of L-phenylalanine which has come to be rapidly used as a starting material of an artificial sweetner "Aspartame".
  • N-acetylphenylalanine readily undergoes asymmetric hydrolysis by the action of an enzyme acylase to form L-phenylalanine.
  • N-acrylphenylalanines have generally been produced by the reduction of either 2-substituted-4-(substituted) benzylidene-5-oxazolones, which are produced relatively easily by a condensation reaction of an N-acylglycine and a benzaldehyde, or ⁇ -acylaminocinnamic acids which are hydrolysis products of said oxazolones.
  • noble metals such as palladium or platinum have also come to be used frequently on an industrial scale as catalysts for use in catalytic reduction. This is because these catalysts are effective in relatively small amounts, are recovered easily because they are heterogeneous to various solvents, and eliminate environmental problems caused by disposal because they are regenerative. They are extremely expensive, however. These expensive noble metal catalysts are generally used repeatedly in industry by way of their recovery after completion of reaction.
  • the present inventors have hydrolyzed 2-methyl-4-benzylidene-5-oxazolone with alkali in water and reduced catalytically the resultant of-acetylamino- cinnamic acid, without isolating it from the reaction solution, by adding a palladium or platinum reducing catalyst thereto to prepare N-acetylphenylalanine. It was then found that the catalyst recovered by filtration after the reduction was lowered in catalytic activity through use, thus requiring considerably longer times in effecting subsequent reductions compared with reductions performed with fresh catalyst. Finally, the noble metal catalyst lost its activity almost completely after several repeated uses thereof.
  • the present invention provides a process for producing N-acylphenylalanines represented by the general formula (II): wherein R 4 and R 5 independently of one another are a hydrogen atom, an alkyl group having 1 - 4 carbon atoms, an alkoxy group having 1 - 4 carbon atoms, a halogen atom, a hydroxyl group or an aryloxy group, or in combination are the methylenedioxy group, and R 3 is a methyl or phenyl group, which process comprises hydrolyzing, with alkali, 2-substituted-4-(substituted) benzylidene-5-oxazolones represented by the general formula (I): wherein each R and R 2 independently of one another are a hydrogen atom, an alkyl group having 1 - 4 carbon atoms, an alkoxy group having 1 - 4 carbon atoms, a halogen atom, an acyloxy group or an aryloxy group, or adjoiningly to each other
  • the time period required for the reduction can be remarkably shortened compared with the time required for reduction in an aqueous, strong alkaline solution. Moreover, there is such a great advantage in the present process that the catalyst recovered through filtration after the reduction can be used repeatedly without any additional treatment and without any observed decrease in its catalytic activity. A reduction using the catalyst recovered from a previous reduction may proceed at practically the same rate as in the case of a reduction performed using fresh catalyst.
  • the starting material 2-substituted -4-(substituted) benzylidene-5-oxazolone
  • the present process has advantages over a process wherein a N-acylaminocinnamic acid is reduced after it has been isolated from the reaction system; the advantages include the benelit-of process simplification and surprisingly improved overall yield.
  • R 1 and R are univalent, they can be attached to any unoccupied position on the benzylidene ring. If R 1 and R 2 (and R 3 and R 4 ) are combined as the divalent methylenedioxy group, however, this group attaches to two adjoining positions of the benzylidene ring.
  • the process of the present invention comprises two steps consisting of (A) treating a 2-substituted-4-(substituted) benzylidene-5-oxazolone represented by the general formula (I) with alkali to form a substituted or unsubstituted ⁇ -acylaminocinnamic acid and (B) reducing catalytically the resultant ⁇ -acylaminocinnamic acid without isolating it from the reaction system.
  • 2-substituted-4-(substituted)benzylidene-5-oxazolone of the general formula (I) used as the starting material in the present process include 2-methyl-4-benzylidene-5-oxazolone, 2-phenyl-4-benzylidene-5-oxazolone, 2-methyl-4-(p-methylbenzylidene)-5-oxazolone, 2-phenyl-4-(p-methylbenzylidene)-5-oxazolone, 2-methyl-4-(p-ethylbenzylidene)-5-oxazolone, 2-phenyl-4-(p-iso-propylbenzylidene)-5-oxazolone, 2-methyl-4-(p-n-butylbenzylidene)-5-oxazolone, 2-methyl-4-(p-methoxybenzylidene)-5-oxazolone, 2-phenyl-4-(p-methoxybenzylidene)-5-oxazol
  • the first step of the process of the present invention in which an ⁇ -acylaminocinnamic acid is produced through hydrolysis of a 2-substituted-4-(substituted)-benzylidene-5-oxazolone, comprises treating this starting material present in an aqueous medium in a state of suspension or solution by adding thereto an alkali such as the hydroxide, oxide or carbonate of an alkali or alkaline earth metal in amount in excess of its stoichiometriciequirement, thereby producing a corresponding ⁇ -acylaminocinnamic acid easily.
  • an alkali such as the hydroxide, oxide or carbonate of an alkali or alkaline earth metal
  • the amount of water used is one part by weight or more, or preferably two parts by weight or more from an operational standpoint, per one part by weight of the 2-substituted-4-(substituted) benzylidene-5-oxazolone starting material.
  • the temperature and time duration applied in the hydrolysis are 0 - 1000C and 0.5 - 20 hours, or preferably 20 - 80°C and 1 - 15 hours, respectively.
  • An aqueous solution of an alkali or alkaline earth metal salt of an ⁇ -acylaminocinnamic acid is obtained in the above manner.
  • the acyloxy group will also be hydrolyzed to form a hydroxyl group-substituted ⁇ -acylaminocinnamic acid.
  • various water-miscible organic solvents for example, methanol, ethanol, isopropanol, acetone, dioxane, tetrahydrofuran or the like, may be used jointly without raising any especial problems.
  • the hydrolysis reaction proceeds under mild conditions even in an aqueous medium.
  • the reaction solution containing an alkaline salt of an oC-acylaminocinnamic acid resulting from the foregoing first step is adjusted in pH to 5 - 9 and thereafter it is subjected to catalytic reduction in the presence of a palladium or platinum reducing catalyst to produce an N-acylphenylalanine.
  • the said reaction solution is neutralized with an acid to adjust its pH in the range of 5 - 9 or preferably 5.5 - 8.5 and subsequently exposed to a reducing catalyst so as to be subjected to catalytic reduction.
  • an acid to adjust its pH in the range of 5 - 9 or preferably 5.5 - 8.5 and subsequently exposed to a reducing catalyst so as to be subjected to catalytic reduction.
  • hydrochloric and sulfuric acids are frequently used for the pH adjustment, there may also be used, as a matter of course, other mineral acids or organic acids such as acetic acid and p-toluenesulfonic acid.
  • the pH of the reaction solution should exceed 9 during the reduction period, the catalytic activity of the recovered catalyst will, as pointed out previously, be lowered or lost through its repeated use, and even in the case of using a fresh catalyst, the reaction time duration will tend to be prolonged as opposed to the reduction carried out in the pH range of 5 to 9. Further, if the pH should be below 5, the solubility of the N-acylaminocinnamic acid will be decreased and the reduction will have to be carried out in a state of suspension so that the time taken for completion of the reaction will be prolonged unfavourably.
  • any type of noble metal catalyst of the palladium or platinum series can be used as the reducing catalyst so long as it makes up a heterogenous catalytic system to the reaction solution.
  • specific examples of such catalysts may include palladium-carbon, palladium black, colloidal palladium, palladium-barium sulfate, palladium-alumina, platinum oxide, platinum-carbon or platinum-silica gel.
  • the catalyst used in practising the present invention is not limited to these particular, exemplary catalyst, however.
  • the amount of the catalyst to be used is generally 0.1% by weight or more based on the weight of the starting material,-2-substituted-4-(substituted)-benzylidene-5-oxazolone.
  • the catalyst used, the shorter will be the time required for the reduction. However, from economical and operational points of view, it is preferred to use the catalyst in an amount of 30% by weight or less. More favourably, it is recommended to use it in an amount in the range of 0.5 - 10% by weight.
  • the temperature and the duration of the reduction depend more or less on the amount of the catalyst used but generally lie in the ranges of 0 - 100 0 C and 0.5 - 30 hours, respectively.
  • the reduction may be effected either at atmospheric pressure or under pressure.
  • N-acylphenylalanines are generally dissolved in the reaction solution after completion of the reaction, it is possible, as required, to isolate the N-acylphenylalanines from the reaction solution by filtering and removing the catalyst while the reaction solution is hot and thereafter acidifying the filtrate with an acid such as hydrochloric acid.
  • the catalyst thus-recovered can be used repeatedly, maintaining its original activity to effect the reduction without any further treatment and without any observed lowering in its catalytic activity.
  • the gas phase in the vessel was purged with nitrogen and then with hydrogen and thereafter the vessel's contents were subjected to catalytic reduction at 40 - 45°C under atmospheric pressure.
  • the reaction time was approximately 90 minutes until hydrogen absorption was completed. During this time period, it was observed that one mole of hydrogen was absorbed to one mole of 2-methyl-4-benzylidene-5-oxazolone.
  • the gas phase in the vessel was purged with nitrogen after completion of the reaction and thereafter the catalyst was filtered and washed with a small amount of water.
  • the filtrate and the washings were combined and concentrated hydrochloric acid was added thereto at 30 - 35°C to adjust pH of the resultant mixture to 1.
  • the mixture was then cooled to 0 - 5°C, The resulting crystals were separated by filtering, were washed with cold water and dried to obtain 9.96 g of N-acetylphenylalanine as a white crystal. Its yield was 96.1% based on the 2-methyl-4-benzylidene-5-oxazolone, and its melting point was 150.5 - 151°C.
  • reaction mixture in the vessel was subjected to catalytic reduction at 40 - 45°C under atmospheric pressure. The time required for the reduction was approximately four hours. After completion of the reduction, the reaction mixture was treated in the same manner as in Example 1 to recover the catalyst and obtain 9.91 g of N-acetylphenylalanine as a white crystal. Its melting point was 149.5 - 150.5 0 C .
  • Example 2 Each example was effected in the same manner as in Example 1 except that the pH of the solution during the reduction was changed. Results are shown in Table 2. Each catalyst recovered in these two experiments was used repeatedly three times under the same respective conditions. The time durations required for the reduction of these repetitions were practically the same as that resulted when a fresh catalyst was used,

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Abstract

An N-acyl-substituted or unsubstituted phenylalanine is prepared by hydrolyzing a 2-substituted-4-substituted or unsubstituted benzylidene-5-oxazolone with an alkali, adjusting the reaction system containing its hydrolysis product with acid to a pH of 5 - 9 and reducing the resultant reaction solution catalytically in the presence of a palladium or platinum reducing catalyst. The reduction is carried out continuously without isolating the alkaline hydrolysis product, i.e., a substituted or unsubstituted N-acylaminocinnamic acid from the reaction system. …<??>The catalyst can be recovered after completion of the reduction and used repeatedly without additional treatment and without any observed lowering in its catalytic activity. Accordingly, the reductions using the recovered catalyst may proceed at pratically the same rate as reductions using a fresh catalyst.

Description

  • This invention relates to a process for producing substituted or unsubstituted N-acylphenylalanines.
  • N-acylphenylalanines are important compounds as precursors of phenylalanines. In particular, unsubstituted N-acylphenylalanines are categorized as essential amino acids and are important compounds as precursors of L-phenylalanine which has come to be rapidly used as a starting material of an artificial sweetner "Aspartame". For example, N-acetylphenylalanine readily undergoes asymmetric hydrolysis by the action of an enzyme acylase to form L-phenylalanine.
  • Conventionally, N-acrylphenylalanines have generally been produced by the reduction of either 2-substituted-4-(substituted) benzylidene-5-oxazolones, which are produced relatively easily by a condensation reaction of an N-acylglycine and a benzaldehyde, or α-acylaminocinnamic acids which are hydrolysis products of said oxazolones. h
  • Although various processes have hitherto been proposed for effecting the reduction, it is practical in an industrial sense to carry out the reduction catalytically in the presence of a heterogeneous reducing catalyst. For example, in accordance with the method of T. Okuda and Y. Fugii (Bull. Chem. Soc. (Japan), 30, 698 (1957)), a substituted or unsubstituted 2-methyl-4-benzylidene-5-oxazolone is reduced catalytically in an alkaline solution under a pressure of 40 - 70 kg/cm2 using Raney nickel as a catalyst to produce an N-acetylphenylalanine. As an example of using a heterogeneous catalyst of noble metal, there is disclosed a method by R.M. Herbst and D. Shemin (Organic Synthesis, Coll. Vol. 2, p491) wherein α-acetylaminocinnamic acid is reduced catalytically in acetic acid under atmospheric pressure using platinum oxide as a catalyst to produce N-acetylphenylalanine.
  • However, since the former method carries out the reduction under a high pressure, restrictions are imposed on the apparatus that is used in effecting the method industrially. Moreover, the Raney nickel is used in a relatively large amount and thus raises environmental problems in its disposal. The prior method which uses acetic acid as a solvent is accompanied by drawbacks, e.g. that its operation is complicated because the solvent has to be concentrated or distilled upon isolation of the product after the reduction.
  • Recently, noble metals such as palladium or platinum have also come to be used frequently on an industrial scale as catalysts for use in catalytic reduction. This is because these catalysts are effective in relatively small amounts, are recovered easily because they are heterogeneous to various solvents, and eliminate environmental problems caused by disposal because they are regenerative. They are extremely expensive, however. These expensive noble metal catalysts are generally used repeatedly in industry by way of their recovery after completion of reaction.
  • The present inventors have hydrolyzed 2-methyl-4-benzylidene-5-oxazolone with alkali in water and reduced catalytically the resultant of-acetylamino- cinnamic acid, without isolating it from the reaction solution, by adding a palladium or platinum reducing catalyst thereto to prepare N-acetylphenylalanine. It was then found that the catalyst recovered by filtration after the reduction was lowered in catalytic activity through use, thus requiring considerably longer times in effecting subsequent reductions compared with reductions performed with fresh catalyst. Finally, the noble metal catalyst lost its activity almost completely after several repeated uses thereof. The same situation occured when other 2-substituted-4-(substituted) benzylidene-5-oxazolones were used. Further, it was also found that a catalyst whose activity had been lowered or lost could not be restored as to its catalytic activity even by washing it with an organic solvent, such as alcohol, or with an acid such as dilute hydrochloric acid and thus its catalytic activity was irreversibly degraded.
  • On the basis of these experimental facts, the present inventors have made an intensive effort to establish a process for the preparation of N-acylphenylalanines from 2-substituted-4-(substituted) benzylidene-5-oxazolones wherein a reduction catalyst could be used efficiently and repeatedly without its catalytic activity being lowered or lost during the reduction. As a result of their work, it was found that the pH of the reaction solution during the reduction was closely related to the activity of the catalyst and was responsible for the irreversible decrease in activity of the recovered catalyst, thus leading to the completion of the present invention.
  • Accordingly, the present invention provides a process for producing N-acylphenylalanines represented by the general formula (II):
    Figure imgb0001
    wherein R 4 and R5 independently of one another are a hydrogen atom, an alkyl group having 1 - 4 carbon atoms, an alkoxy group having 1 - 4 carbon atoms, a halogen atom, a hydroxyl group or an aryloxy group, or in combination are the methylenedioxy group, and R3 is a methyl or phenyl group, which process comprises hydrolyzing, with alkali, 2-substituted-4-(substituted) benzylidene-5-oxazolones represented by the general formula (I):
    Figure imgb0002
    wherein each R and R2 independently of one another are a hydrogen atom, an alkyl group having 1 - 4 carbon atoms, an alkoxy group having 1 - 4 carbon atoms, a halogen atom, an acyloxy group or an aryloxy group, or adjoiningly to each other and together are a methylenedioxy group, and R 3 is a methyl or phenyl group, adjusting the pH of the reaction solution containing the hydrolysis product to 5 - 9 with acid and subjecting the resultant reaction solution to catalytic reduction in the presence of a palladium or platinum reduction catalyst.
  • In performing the process of the present invention, the time period required for the reduction can be remarkably shortened compared with the time required for reduction in an aqueous, strong alkaline solution. Moreover, there is such a great advantage in the present process that the catalyst recovered through filtration after the reduction can be used repeatedly without any additional treatment and without any observed decrease in its catalytic activity. A reduction using the catalyst recovered from a previous reduction may proceed at practically the same rate as in the case of a reduction performed using fresh catalyst. Further, in the process of the present invention, the starting material, 2-substituted -4-(substituted) benzylidene-5-oxazolone, is hydrolyzed with alkali to produce a substituted or unsubstituted N-acylaminocinnamic acid which is subsequently and continuously subjected to the reduction, that is to say without being isolated from the reaction mixture. Accordingly, the present process has advantages over a process wherein a N-acylaminocinnamic acid is reduced after it has been isolated from the reaction system; the advantages include the benelit-of process simplification and surprisingly improved overall yield.
  • If R1 and R (and correspondingly R3 and R ) are univalent, they can be attached to any unoccupied position on the benzylidene ring. If R1 and R 2 (and R 3 and R 4) are combined as the divalent methylenedioxy group, however, this group attaches to two adjoining positions of the benzylidene ring.
  • The invention is now explained in more detail in the following non-limitative description of a preferred mode of carrying out the process.
  • The process of the present invention comprises two steps consisting of (A) treating a 2-substituted-4-(substituted) benzylidene-5-oxazolone represented by the general formula (I) with alkali to form a substituted or unsubstituted α-acylaminocinnamic acid and (B) reducing catalytically the resultant α-acylaminocinnamic acid without isolating it from the reaction system.
  • Specific examples of the 2-substituted-4-(substituted)benzylidene-5-oxazolone of the general formula (I) used as the starting material in the present process include 2-methyl-4-benzylidene-5-oxazolone, 2-phenyl-4-benzylidene-5-oxazolone, 2-methyl-4-(p-methylbenzylidene)-5-oxazolone, 2-phenyl-4-(p-methylbenzylidene)-5-oxazolone, 2-methyl-4-(p-ethylbenzylidene)-5-oxazolone, 2-phenyl-4-(p-iso-propylbenzylidene)-5-oxazolone, 2-methyl-4-(p-n-butylbenzylidene)-5-oxazolone, 2-methyl-4-(p-methoxybenzylidene)-5-oxazolone, 2-phenyl-4-(p-methoxybenzylidene)-5-oxazolone, 2-methyl-4-(3,4-dimethoxybenzylidene)-5-oxazolone, 2-methyl-4-(2,3-dimethoxybenzylidene)-5-oxazolone, 2-phenyl-4-(2,4-dimethoxybenzylidene)-5-oxazolone, 2-methyl-4-(3,5-dimethoxybenzylidene)-5-oxazolone, 2-methyl-4-(p-ethoxybenzylidene)-5-oxazolone, 2-methyl-4-(3,4-diethoxybenzylidene)-5-oxazolone, 2-phenyl-4-(p-n-propoxybenzylidene)-5-oxazolone, 2-methyl-4-(p-n-butoxybenzylidene)-5-oxazolone, 2-methyl-4-(p-chlorobenzylidene)-5-oxazolone, 2-phenyl-4-(p-chorobenzylidene)-5-oxazolone, 2-methyl-4-(3,4-dichlorobenzylidene)-5-oxazolone, 2-phenyl-4-(3,4-dichlorobenzylidene)-5-oxazolone, 2-methyl-4-(m-phenoxybenzylidene)-5-oxazolone, 2-phenyl-4-(m-phenoxybenzylidene)-5-oxazolone, 2-methyl-4-(p-acetoxybenzylidene)-5-oxazolone, 2-phenyl-4-(p-acetoxybenzylidene)-5-oxazolone, 2-methyl-4-(3,4-diacetoxybenzylidene)-5-oxazolone, 2-phenyl-4-(3,4-diacetoxybenzylidene)-5-oxazolone, 2-methyl-4-(3,4-methylenedioxybenzylidene)-5-oxazolone or 2-phenyl-4-(3,4-methylenedioxybenzylidene)-5-oxazolone. These starting materials can easily be produced by the Elrenmeyer method in which N-acetylglycine or N-benzoylglycine (hippuric acid) is condensed with a substituted or unsubstituted benzaldehyde in acetic anhydride in the presence of anhydrous sodium acetate. Another method involves treating a p-phenylserine in acetic anhydride in the presence of a base (Japanese Patent Application Laid-Open No. 32753/1985).
  • The first step of the process of the present invention, in which an α-acylaminocinnamic acid is produced through hydrolysis of a 2-substituted-4-(substituted)-benzylidene-5-oxazolone, comprises treating this starting material present in an aqueous medium in a state of suspension or solution by adding thereto an alkali such as the hydroxide, oxide or carbonate of an alkali or alkaline earth metal in amount in excess of its stoichiometriciequirement, thereby producing a corresponding α-acylaminocinnamic acid easily. The amount of water used is one part by weight or more, or preferably two parts by weight or more from an operational standpoint, per one part by weight of the 2-substituted-4-(substituted) benzylidene-5-oxazolone starting material. The temperature and time duration applied in the hydrolysis are 0 - 1000C and 0.5 - 20 hours, or preferably 20 - 80°C and 1 - 15 hours, respectively. An aqueous solution of an alkali or alkaline earth metal salt of an α-acylaminocinnamic acid is obtained in the above manner. However, in a case where an acyloxy group-substituted oxazolone is used as the starting material, the acyloxy group will also be hydrolyzed to form a hydroxyl group-substituted α-acylaminocinnamic acid. In the hydrolysis of 2-substituted-4-(substituted) benzylidene-5-oxazolones, various water-miscible organic solvents, for example, methanol, ethanol, isopropanol, acetone, dioxane, tetrahydrofuran or the like, may be used jointly without raising any especial problems. However, the hydrolysis reaction proceeds under mild conditions even in an aqueous medium. It may sometimes be necessary to distill or remove such an organic solvent in the isolation of the intended compound of N-acrylphenyl- alanine after completion of the subsequent catalytic reduction, so that the post-reaction treatment is made complicated. From this point of view, it is not usually preferred to employ an organic solvent in the aqueous medium.
  • In the second step of the process of the present invention, the reaction solution containing an alkaline salt of an oC-acylaminocinnamic acid resulting from the foregoing first step is adjusted in pH to 5 - 9 and thereafter it is subjected to catalytic reduction in the presence of a palladium or platinum reducing catalyst to produce an N-acylphenylalanine.
  • For effecting the catalytic reduction of this step, the said reaction solution is neutralized with an acid to adjust its pH in the range of 5 - 9 or preferably 5.5 - 8.5 and subsequently exposed to a reducing catalyst so as to be subjected to catalytic reduction. Although hydrochloric and sulfuric acids are frequently used for the pH adjustment, there may also be used, as a matter of course, other mineral acids or organic acids such as acetic acid and p-toluenesulfonic acid. If the pH of the reaction solution should exceed 9 during the reduction period, the catalytic activity of the recovered catalyst will, as pointed out previously, be lowered or lost through its repeated use, and even in the case of using a fresh catalyst, the reaction time duration will tend to be prolonged as opposed to the reduction carried out in the pH range of 5 to 9. Further, if the pH should be below 5, the solubility of the N-acylaminocinnamic acid will be decreased and the reduction will have to be carried out in a state of suspension so that the time taken for completion of the reaction will be prolonged unfavourably.
  • Any type of noble metal catalyst of the palladium or platinum series can be used as the reducing catalyst so long as it makes up a heterogenous catalytic system to the reaction solution. Specific examples of such catalysts may include palladium-carbon, palladium black, colloidal palladium, palladium-barium sulfate, palladium-alumina, platinum oxide, platinum-carbon or platinum-silica gel. The catalyst used in practising the present invention is not limited to these particular, exemplary catalyst, however. The amount of the catalyst to be used is generally 0.1% by weight or more based on the weight of the starting material,-2-substituted-4-(substituted)-benzylidene-5-oxazolone. The more catalyst used, the shorter will be the time required for the reduction. However, from economical and operational points of view, it is preferred to use the catalyst in an amount of 30% by weight or less. More favourably, it is recommended to use it in an amount in the range of 0.5 - 10% by weight.
  • The temperature and the duration of the reduction depend more or less on the amount of the catalyst used but generally lie in the ranges of 0 - 1000 C and 0.5 - 30 hours, respectively. The reduction may be effected either at atmospheric pressure or under pressure.
  • Since N-acylphenylalanines are generally dissolved in the reaction solution after completion of the reaction, it is possible, as required, to isolate the N-acylphenylalanines from the reaction solution by filtering and removing the catalyst while the reaction solution is hot and thereafter acidifying the filtrate with an acid such as hydrochloric acid. The catalyst thus-recovered can be used repeatedly, maintaining its original activity to effect the reduction without any further treatment and without any observed lowering in its catalytic activity.
  • The present invention will be described more specifically with reference to the following non-limiting examples.
  • Example 1:
  • In a 100-ml tightly-sealed glass vessel were charged 9.36 g of 2-methyl-4-benzylidene-5-oxazolone and 30 ml of water. Thereafter, 5.3 g of 45% sodium hydroxide solution was added thereto and the resulting mixture was stirred at 40 - 45°C for 2 hours. The 2-methyl-4-benzylidene-5-oxazolone was hydrolyzed to form an aqueous homogeneous solution of sodium α-acetylaminocinnamate. Then, concentrated hydrochloric acid was added to the solution to adjust its pH to 7.2, and subsequently 0.2 g of 5%-palladium-carbon was added thereto. The gas phase in the vessel was purged with nitrogen and then with hydrogen and thereafter the vessel's contents were subjected to catalytic reduction at 40 - 45°C under atmospheric pressure. The reaction time was approximately 90 minutes until hydrogen absorption was completed. During this time period, it was observed that one mole of hydrogen was absorbed to one mole of 2-methyl-4-benzylidene-5-oxazolone.
  • The gas phase in the vessel was purged with nitrogen after completion of the reaction and thereafter the catalyst was filtered and washed with a small amount of water. The filtrate and the washings were combined and concentrated hydrochloric acid was added thereto at 30 - 35°C to adjust pH of the resultant mixture to 1. The mixture was then cooled to 0 - 5°C, The resulting crystals were separated by filtering, were washed with cold water and dried to obtain 9.96 g of N-acetylphenylalanine as a white crystal. Its yield was 96.1% based on the 2-methyl-4-benzylidene-5-oxazolone, and its melting point was 150.5 - 151°C.
  • Example 2:
  • In the same manner as in Example 1, 2-methyl-4-benzylidene-5-oxazolone was treated with alkali to obtain an aqueous solution of sodium N-acetylamino- cinnamate which was then subjected to reduction, after adjusting its pH with concentrated hydrochloric acid to 7.2, using the palladium-carbon catalyst recovered in Example 1 without any further treatment. The catalyst was used repeatedly five times for effecting the reduction. Results are shown in Table 1. In each of the repeated experiments, the reduction was completed in 95 - 105 minutes. This signifies in comparison with Example 1 that each reduction time was not, in practical terms, affected by the repeated use of catalyst.
    Figure imgb0003
  • Comparative Example 1:
  • In a 100-ml tightly-sealed glass vessel were charged 9.36 g of 2-methyl-4-benzylidene-5-oxazolone and 30 ml of water. Then, 5.3 g of 45% sodium hydroxide solution was added thereto and the resulting mixture was stirred at 40 - 450C for two hours. The 2-methyl-4-benzylidene-5-oxazolone was hydrolyzed to form an aqueous homogeneous solution of sodium α-acetylaminocinnamate. The solution had a pH in excess of 12. 0.2 g of 5%-palladium-carbon was then added to the solution. After the gas phase in the vessel was purged with nitrogen and then with hydrogen, the reaction mixture in the vessel was subjected to catalytic reduction at 40 - 45°C under atmospheric pressure. The time required for the reduction was approximately four hours. After completion of the reduction, the reaction mixture was treated in the same manner as in Example 1 to recover the catalyst and obtain 9.91 g of N-acetylphenylalanine as a white crystal. Its melting point was 149.5 - 150.50 C.
  • Comparative Example 2:
  • Procedures of Comparative Example 1 were repeated to effect the reaction except for the repeated use of the catalyst recovered in Comparative Example 1. The time durations required for the reduction were 6.5 hours and 10 hours for the first and second repeated uses, respectively. In the third repeated use, hydrogen absorption was interrupted during the reduction.
  • Examples 3 and 4:
  • Each example was effected in the same manner as in Example 1 except that the pH of the solution during the reduction was changed. Results are shown in Table 2. Each catalyst recovered in these two experiments was used repeatedly three times under the same respective conditions. The time durations required for the reduction of these repetitions were practically the same as that resulted when a fresh catalyst was used,
    Figure imgb0004
  • Example 5:
  • In 100-ml tightly-sealed glass vessel were charged 9.36 g of 2-methyl-4-benzylidene-5-oxazolone and 40 ml of water. 4.15 g of potassium carbonate was then added thereto and the resulting mixture was stirred at 40 - 450C for two hours. Concentrated hydrochloric acid was added to the resulting solution to adjust its pH to 6.8 and 0.2 g of 5%- palladium-carbon was added thereto. The gas phase in the vessel was purged with nitrogen and then with hydrogen, and thereafter the reaction mixture in the vessel was subjected to catalytic reduction at 40 - 45°C under atmospheric pressure. The reaction time during which hydrogen absorption was completed was 100 minutes. It was observed that one mole of hydrogen was absorbed to one mole of 2-methyl-4-benzylidene-5-oxazolone during this period. After the gas phase in the vessel was purged with nitrogen upon completion of the reaction, the catalyst was filtered and washed with a small amount of water. The filtrate and the washings were combined, and concentrated hydrochloric acid was added at a temperature lower than 30°C to adjust the pH of the resulting mixture to 1. The mixture was cooled to 0 - 5°C. Crystals formed and were separated by filtration, were washed with cold water and dried to obtain 9.81 g of N-acetylphenylalanine having a melting point of 150 - 151°C as a white crystal. The yield was 94.7%.
  • Using the recovered catalyst repeatedly, experiments were carried out three times under the same conditions as described above. In each of the experiments, the time duration required for the reduction was in the range of 95 - 110 minutes which was practically the same as in the case of using a fresh catalyst.
  • Examples 6 to 12:
  • 0.05 mole of each of various 2-substituted-4-(substituted) benzylidene-5-oxazolones was suspended in 40 - 100 ml of water and 5.3 g of 45% sodium hydroxide solution was added thereto to hydrolyze it at 40 - 80°C for 1 - 3 hours, thereby forming a corresponding d-acylaminocinnamic acid. Then concentrated hydrochloric acid was added to the resultant aqueous solution to adjust its pH to 5.5 - 8.5. The resultant solution was charged into a tightly-sealed glass vessel. 5%- palladium-carbon was added thereto and the gas phase in the vessel was purged with nitrogen and with hydrogen. Thereafter, the reaction mixture was subjected to catalytic reduction under atmospheric pressure. Procedures of Example 1 were repeated in the isolation of each of N-acylphenylalanines resulted from the reduction. Results are shown in Table 3.
    Figure imgb0005

Claims (9)

1, A process for producing an N-acylphenylalanine represented by the general formula (II):
Figure imgb0006
wherein R4 and R 5 independently of one another are a hydrogen atom, an alkyl group having 1 - 4 carbon atoms, an alkoxy group having 1 - 4 carbon atoms, a halogen atom, a hydroxyl group or an aryloxy group, or in combination are the methylenedioxy group, and R3 is a methyl or phenyl group, the process comprising hydrolyzing with alkali a 2-substituted-4-(substituted)benzylidene-5-oxazolone represented by the general formula (I) :
Figure imgb0007
wherein R1 and R2 independently of one another are a hydrogen atom, an alkyl group having 1 - 4 carbon atoms, an alkoxy group having 1 - 4 carbon atoms, a halogen atom, an acyloxy group or an aryloxy group, or in combination are the methylenedioxy group, and R3 is a methyl or phenyl group, adjusting the pH of the reaction solution containing the hydrolysis product to 5 - 9 with acid, and subjecting the resultant reaction solution to catalytic reduction in the presence of a palladium or a platinum reduction catalyst.
2. A process according to claim 1, wherein the alkali is a hydroxide, oxide or carbonate of an alkali metal or an alkaline earth metal.
3. A process according to claim 1 or claim 2, wherein the hydrolysis reaction is carried out at a temperature in the range 20 - 80°C.
4. A process according to claim 1, 2 or 3, wherein one part by weight of the starting substance comprising the 2-substituted-4-(substituted)benzylidene-5-oxazolone is dissolved or suspended in 2 or more parts by weight of water for the hydrolysis reaction.
5. A process according to claim 1, 2, 3 or 4, wherein the reaction solution containing the hydrolysis product is adjusted to a pH in the range 5.5 to 8.5.
6. A process according to any of claims 1 to 5, wherein the catalyst is selected from palladium-carbon, palladium black, colloidal palladium, palladium-barium sulfate, palladium-alumina, platinum oxide, platinum-carbon and platinum-silica gel.
7. A process according to any of claims 1 to 6, wherein the catalyst is used in an amount of 0.1 to 30% by weight, based on the weight of the starting substance comprising the 2-substituted-4-(substituted)benzylidene-5-oxazolone, and preferably in an amount of 0.5 to 10% by weight on the same basis.
8. A process for the manufacture of L-phenylalanines, comprising preparing an N-acylphenylalanine by the process according to any of claims 1 to 7, and then subjecting the N-acylphenylalanine to asymmetric hydrolysis by the action of an enzyme acylase.
9. An artificial sweetner comprising aspartame prepared from the product of the process according to claim 8.
EP85302503A 1984-04-10 1985-04-09 Process for producing n-acylphenylalanines Expired EP0161796B1 (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2591070A1 (en) * 1985-12-06 1987-06-12 Budapesti Vegyimuevek NOVEL NON-SATURATED AZLACTONE DERIVATIVES, PROCESS FOR THEIR PREPARATION AND PESTICIDE COMPOSITIONS COMPRISING SAME
GB2188047A (en) * 1986-03-04 1987-09-23 Toyo Jozo Kk Aldose reductase inhibitors

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63502112A (en) * 1986-01-03 1988-08-18 コンソリデイテツド・フアーマシユーテイカルズ・リミテツド Melphalan derivative
DE3736861A1 (en) * 1987-10-30 1989-05-11 Hoechst Ag METHOD FOR PRODUCING N-ACETYLPHENYLALANINE
IT1226903B (en) * 1988-07-12 1991-02-21 Mini Ricerca Scient Tecnolog PROCESS FOR THE SYNTHESIS OF OPTICALLY ACTIVE AMINO ACIDS
BR9306656A (en) * 1992-06-30 1998-12-08 Legomer Partners Lp Mimetic composition of nucleotide peptide and substrate compound carbohydrate and process for synthesizing a compound
US5670480A (en) * 1994-01-05 1997-09-23 Arqule, Inc. Method of making polymers having specific properties
TW200624151A (en) * 2004-11-12 2006-07-16 Monsanto Technology Llc Recovery of noble metals from aqueous process streams
CN101684077B (en) 2008-09-24 2013-01-02 浙江九洲药业股份有限公司 Method for preparing N-acyl diphenylalanine
CA2772681C (en) * 2009-09-23 2017-01-03 Zhejiang Jiuzhou Pharmaceutical Co., Ltd. Process for manufacture of n-acylbphenyl alanine
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Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3838008A (en) * 1970-01-19 1974-09-24 Astra Laekemedel Ab Stereoselective preparation of l-dopa and l-m-tyrosine and novel compounds

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2678313A (en) * 1954-05-11 Stable molecular compounds of
US3085111A (en) * 1957-03-19 1963-04-09 Warner Lambert Pharmaceutical Process for preparing 3-nitrobenzaldehyde ethers
CH441362A (en) * 1963-12-24 1967-08-15 Hoffmann La Roche Process for the preparation of phenylalanine derivatives
US3544623A (en) * 1966-05-18 1970-12-01 Warner Lambert Pharmaceutical 3-isopropyltyrosine
US4261919A (en) * 1968-09-09 1981-04-14 Monsanto Company Catalytic asymmetric hydrogenation
US4508921A (en) * 1984-06-28 1985-04-02 Merck & Co., Inc. Process for preparation of alpha-alkyl amino acids

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3838008A (en) * 1970-01-19 1974-09-24 Astra Laekemedel Ab Stereoselective preparation of l-dopa and l-m-tyrosine and novel compounds

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2591070A1 (en) * 1985-12-06 1987-06-12 Budapesti Vegyimuevek NOVEL NON-SATURATED AZLACTONE DERIVATIVES, PROCESS FOR THEIR PREPARATION AND PESTICIDE COMPOSITIONS COMPRISING SAME
GB2188047A (en) * 1986-03-04 1987-09-23 Toyo Jozo Kk Aldose reductase inhibitors
US4749571A (en) * 1986-03-04 1988-06-07 Toyo Jozo Co., Ltd. Physiologically-active novel substance "Aldostatin" and production method thereof
GB2188047B (en) * 1986-03-04 1989-12-28 Toyo Jozo Kk Aldose reductase inhibitor

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