EP0061253A3 - Process for the transfer of genetic material into actinomycete cells, prepared cells and method of culturing these cells and isolating a metabolite - Google Patents

Process for the transfer of genetic material into actinomycete cells, prepared cells and method of culturing these cells and isolating a metabolite

Info

Publication number
EP0061253A3
EP0061253A3 EP82301193A EP82301193A EP0061253A3 EP 0061253 A3 EP0061253 A3 EP 0061253A3 EP 82301193 A EP82301193 A EP 82301193A EP 82301193 A EP82301193 A EP 82301193A EP 0061253 A3 EP0061253 A3 EP 0061253A3
Authority
EP
European Patent Office
Prior art keywords
cells
actinomycete
genetic material
transfer
metabolite
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP82301193A
Other languages
German (de)
French (fr)
Other versions
EP0061253A2 (en
Inventor
John Frank Makins
Geoffrey Holt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Beecham Group PLC
Original Assignee
Beecham Group PLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beecham Group PLC filed Critical Beecham Group PLC
Publication of EP0061253A2 publication Critical patent/EP0061253A2/en
Publication of EP0061253A3 publication Critical patent/EP0061253A3/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/188—Heterocyclic compound containing in the condensed system at least one hetero ring having nitrogen atoms and oxygen atoms as the only ring heteroatoms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)

Abstract

A process for the transfer of genetic material into Actinomycete cells, which comprises entrapment of the genetic material within a lipid vesicle and thereafter fusing the lipid vesicle with an osmotically fragile Actinomycete cell.
EP82301193A 1981-03-18 1982-03-09 Process for the transfer of genetic material into actinomycete cells, prepared cells and method of culturing these cells and isolating a metabolite Withdrawn EP0061253A3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB8108528 1981-03-18
GB8108528 1981-03-18

Publications (2)

Publication Number Publication Date
EP0061253A2 EP0061253A2 (en) 1982-09-29
EP0061253A3 true EP0061253A3 (en) 1982-12-08

Family

ID=10520484

Family Applications (1)

Application Number Title Priority Date Filing Date
EP82301193A Withdrawn EP0061253A3 (en) 1981-03-18 1982-03-09 Process for the transfer of genetic material into actinomycete cells, prepared cells and method of culturing these cells and isolating a metabolite

Country Status (2)

Country Link
EP (1) EP0061253A3 (en)
JP (1) JPS57170188A (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3416978A1 (en) * 1983-05-12 1984-12-06 Stauffer Chemical Co., Westport, Conn. TRANSFORMATION OF EUKARYOTIC CELLS MEDIATED BY LIPOSOME
JPS6030686A (en) * 1983-07-30 1985-02-16 Yakult Honsha Co Ltd Method for introducing donor dna in bacteria belonging to lactobacillus genus
US4789633A (en) * 1984-04-19 1988-12-06 University Of Tennessee Research Corporation Fused liposome and acid induced method for liposome fusion
GB8813055D0 (en) * 1988-06-02 1988-07-06 Beecham Group Plc Novel substance
AU638271B2 (en) * 1989-06-22 1993-06-24 Nexstar Pharmaceuticals, Inc. Encapsulation process
US20020028215A1 (en) 1999-08-09 2002-03-07 Jagath L. Kadurugamuwa Novel vaccines and pharmaceutical compositions using membrane vesicles of microorganisms, and methods for preparing same
RU2117044C1 (en) * 1995-08-25 1998-08-10 Каньчжэн Юрий Владимирович Цзян Device for transmission of natural information supply to biological object
RU2090613C1 (en) * 1995-09-01 1997-09-20 Каньчжэн Юрий Владимирович Цзян Device for communication of natural information supply to biological object
US5773221A (en) * 1995-12-15 1998-06-30 Oceanix Biosciences Corporation Method of recovering a biological molecule from a recombinant microorganism
DE19629271A1 (en) * 1996-07-19 1998-01-22 Wolfgang Prof Dr Piepersberg Isolating DNA from microorganisms that can not be cultured

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
GENE, vol.10, 1980, Elsevier/North-Holland Biomedical Press *
NATURE, vol.268, no.5616, July 14, 1977 *
NATURE, vol.274, July 27, 1978, MacMillan Journals Ltd. *
NATURE, vol.286, no.5772, July 31, 1980, Basingstoke (GB) *
NATURE, vol.293, no.5834, October 22, 1981, Chesham, Bucks (GB) *
NUCLEIC ACIDS RESEARCH, vol.6, no.12, 1979 *
NUCLEIC ACIDS RESEARCH, vol.6, no.8, 1979 *
PROC. NATL. ACAD. SCI., vol.76, no.7, July 1979, (US) *

Also Published As

Publication number Publication date
JPS57170188A (en) 1982-10-20
EP0061253A2 (en) 1982-09-29

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Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

AK Designated contracting states

Designated state(s): BE CH DE FR GB IT NL

PUAL Search report despatched

Free format text: ORIGINAL CODE: 0009013

AK Designated contracting states

Designated state(s): BE CH DE FR GB IT NL

17P Request for examination filed

Effective date: 19821217

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 19850404

RIN1 Information on inventor provided before grant (corrected)

Inventor name: MAKINS, JOHN FRANK

Inventor name: HOLT, GEOFFREY