EP0032469B1 - Pipette à déplacement positif perfectionnée - Google Patents

Pipette à déplacement positif perfectionnée Download PDF

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Publication number
EP0032469B1
EP0032469B1 EP81400023A EP81400023A EP0032469B1 EP 0032469 B1 EP0032469 B1 EP 0032469B1 EP 81400023 A EP81400023 A EP 81400023A EP 81400023 A EP81400023 A EP 81400023A EP 0032469 B1 EP0032469 B1 EP 0032469B1
Authority
EP
European Patent Office
Prior art keywords
pipette
piston
tip
pincer
control rod
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
EP81400023A
Other languages
German (de)
English (en)
French (fr)
Other versions
EP0032469A1 (fr
Inventor
Eric Marteau D'autry
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to AT81400023T priority Critical patent/ATE3248T1/de
Publication of EP0032469A1 publication Critical patent/EP0032469A1/fr
Application granted granted Critical
Publication of EP0032469B1 publication Critical patent/EP0032469B1/fr
Expired legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B01L3/0279—Interchangeable or disposable dispensing tips co-operating with positive ejection means

Definitions

  • the present invention relates to an improvement made to precision positive displacement pipettes intended for the sampling and distribution of small quantities of liquid.
  • such positive displacement precision pipettes are already known which are of the type comprising an essentially tubular pipette body at the lower end of which is arranged a calibrated capillary tube in which it moves in a sealed and against an upward elastic restoring force exerted by a main spring, a piston controlled in its downward movement by a control rod protruding from the upper end of said pipette body, as well as a clamping member of the upper end of the piston, which is of the type with elastic branches deviating radially by displacement against the force exerted by a second spring of resistance much greater than that of the main spring and which is integral in translation with said rod control.
  • Such a positive displacement pipette is for example described in French patent application FR-A-2 446 672 belonging to the applicant.
  • the present improvement made to such a type of positive displacement pipette lies mainly in its particular arrangement intended to allow simultaneous ejection of the capillary tip and of the piston, without the operator having to directly manipulate the capillary and / or the piston. It is clear that, when handling radioactive samples or any other contaminated liquid samples, it is crucial to be able to easily ensure such simultaneous ejection of the capillary tip and of the piston which constitute the only contaminated pipette organs by the liquid withdrawn.
  • the precision pipette with positive displacement is characterized in that it comprises a tip with a calibrated capillary orifice, capable of cooperating by fitting with the free lower end of the pipette body, the orifice of the latter. end allowing the passage of at least a part of the pinching member which, in the vicinity of the end of its downward stroke, comes into contact with a stop formed on the inner surface of the endpiece to ensure the simultaneous ejection of said tip and of the piston which remains trapped in the capillary orifice.
  • the positive displacement pipette comprises an essentially tubular body 10 which, in the particular embodiment described, consists of two separate parts which are secured to each other by screwing.
  • the upper part or housing 12 incorporates for example a mechanism for adjusting and displaying the volume to be withdrawn and distributed, and the lower tubular part 14 is used mainly for guiding the nipping member 16 of the piston 18.
  • the particular mechanism of adjustment and display illustrated in Figures 1 and 2 is not part of the present invention and will therefore not be described here in detail.
  • a calibrated capillary 20 in which moves, in a sealed manner and against an upward elastic restoring force exerted by a main spring 22, a piston 18 controlled in its movement by a control rod 24 projecting from the upper end of the housing 12 of the pipette body 10.
  • the pipette has a tip 26 which has a calibrated through capillary orifice 20 and which is able to cooperate by fitting with the free lower end of the pipette body 10.
  • a tip 26 will be produced in a thermoplastic material chosen to resist the samples with which it comes into contact.
  • the piston 18 has at least one sealing zone 28 coming into intimate contact with the inner surface of the capillary 20.
  • Such a piston 18 can for example advantageously be produced in the form of a stainless steel wire having a coating, for example a Teflon coating, at the level of the sealing zone 28. As shown in FIGS. 1 and 4, such a coating can for example have a general cylindrical shape or else can be equipped at its periphery with at least one annular sealing lip coming into intimate contact with the interior surface of the capillary tube 20.
  • Such a type of pipette also comprises a clamping member 16 of the upper end of the piston 18, which is of the type with elastic branches deviating radially by displacement against the force exerted by a second spring 30 of very high resistance. greater than that of the main spring 22. It is clear that such a clamping member 16 must be secured in translation with the control rod 24 of the pipette.
  • the clamping member 16 is in the form of a self-contained cartridge comprising a clamp proper 32 ending at its lower end by elastic branches 34 and the other end of which 36 is screwed into a clamp support 38.
  • This clamp support 38 is capable of sliding, against the force exerted by the second spring 30 and according to a partial stroke in the upper part of the orifice 40 of the door clamp 42.
  • This latter clamp holder 42 slides itself inside the lower part 14 of the pipette body 10 against the force exerted by the main spring 22.
  • the end of the elastic branches 34 has an outwardly flaring surface capable of being applied tightly against the conical lower opening 44 of the clamp holder 42 and this precisely under the action of the force exerted by the second spring 30.
  • the mounting of the cartridge pr said operation is carried out as follows.
  • the clamp 32 is introduced through the conical lower opening 44 of the clamp holder 42, until the flared lower end of the elastic branches 34 come to bear tightly against the conical lower opening 44 of the clamp holder 42.
  • the second spring 30 is then introduced into the clamp holder 42 by its upper end and around the clamp itself 32, then the clamp holder 38 is screwed onto the upper end 36 of the clamp 32, thereby compressing the second spring 30.
  • the main spring 22 comes to bear fixedly on a shoulder 48 formed on the inner surface of the lower part 14 of the pipette body 10, in the vicinity of its free lower end.
  • the clamp holder 42 comes into upper abutment at its upper free surface 50 against an adjustment screw 52 whose variable vertical position makes it possible to adjust the useful stroke of sampling and dispensing the liquid sample.
  • the cooperation of the control rod 24 and the clamping member 16 takes place at the upper surface of the clamp support 38 which is advantageously in the form of a spherical recess 54 able to cooperate with a lower end in the shape of a ball joint 56 of the control rod 24.
  • a small mounting clearance will be maintained between the ball joint 56 of the control rod 24 and the upper surface of the spherical recess 54 of the clamp holder 42, so as to ensure that the frank limit stop up stroke is made at the contact between the clamp holder 42 and the adjusting screw 52 and not through the control rod 24 itself.
  • the separation of the clamp holder 16 and the rest of the control rod 24 makes it possible, in particular by making use of a ball-and-socket contact between these two elements, to avoid any problem of alignment perfect of the different components of the mobile pipette assembly.
  • an adjustable pipette ie the upper stop of limitation of sampling stroke is adjustable by the operator by acting on the adjustable screw in vertical position 52.
  • the present invention is however not not limited to such a type of adjustable pipette, but may also extend to fixed pipettes, ie to pipettes whose adjusting screw would be replaced by an upstroke limit stop which remains fixed in position relative to the pipette body 10.
  • the stop limiting the downward direction of the useful dispensing stroke of the pipette is carried out at a shoulder 58 formed in the vicinity of the lower part of the clamp holder 42.
  • This shoulder 58 is able to cooperate with the shoulder corresponding 48 formed in the vicinity of the lower end of the pipette body 10.
  • Such a lower limit position of the clamp holder 16 is illustrated in thin dashed lines in FIG. 4. It will be noted that this position is obtained by compression complete with main spring 22.
  • the orifice 60 of the free lower end of the pipette body 10 is arranged so as to allow the passage of at least part of the clamping member 16 which, at the end of the downward stroke, comes into contact with a stop 62 formed on the internal surface of the end piece 26.
  • the ends of the elastic branches 34 of the clamp 32 freely project from the conical lower end 44 of the clamp holder 42.
  • the ejection of the tip 26 of the pipette is therefore obtained by exerting a additional force on the control rod 24, which makes it possible to compress the second spring 30.
  • the compression of this second spring 30 causes, beyond the position illustrated in FIG. 4 ie when the clamp holder 42 is in lower stop, in a first step the spacing of the elastic branches 34 which allows the release of the piston 18.
  • the support of the free ends of the elastic branches 34, in the separated state, on the stop 62 formed on the inner surface of the nozzle 26 then performs the actual ejection of the nozzle 26 in the capillary orifice 20 of which the piston 18 remains trapped.
  • the stop 62 formed on the interior surface of the endpiece 26 is in the form of a surface in inward recess.
  • said piston and its sealing zone 28 are arranged such that at the end of the complete travel of the control rod 24, the piston 18 does not escape from the capillary orifice 20 through the free lower end of the latter.
  • the inner surface of the upper part of the endpiece 26 has at least one annular zone 64 projecting or recessed capable of cooperating elastically with an annular zone 66 corresponding recessed or protruding formed on the outer lateral surface of the lower part 14 of the pipette body 10.
  • These annular zones 64, 66 projecting or recessed have been arranged so as to allow a large guide range of the nozzle. 26 on the end of the pipette, for example of the order of 5 to 10 mm and preferably from 6 to 8 mm.
  • these annular zones 64, 66 projecting or recessed are also arranged so as to ensure a short stroke of engagement and disengagement of the endpiece 26, of the order of 2 mm.
  • the compression of the second spring 30 firstly results in a free travel of the order of 5 tenths of a millimeter during which the elastic branches 34 of the clamp 32 move apart. and release the piston 18. Then, when one continues to compress the second spring 30, one obtains a second stroke of the order of the order of 2.5 mm which ensures precisely, by contacting the ends of the branches spaced apart 34 from the clamp 32 with the abutment surface 62 inside the endpiece 26, exceeding this disengagement stroke of the endpiece which is of the order of 2 mm.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
EP81400023A 1980-01-11 1981-01-09 Pipette à déplacement positif perfectionnée Expired EP0032469B1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AT81400023T ATE3248T1 (de) 1980-01-11 1981-01-09 Pipette mit positiver verschiebung.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR8000605 1980-01-11
FR8000605A FR2474340A1 (fr) 1980-01-11 1980-01-11 Pipette a deplacement positif perfectionnee

Publications (2)

Publication Number Publication Date
EP0032469A1 EP0032469A1 (fr) 1981-07-22
EP0032469B1 true EP0032469B1 (fr) 1983-05-11

Family

ID=9237457

Family Applications (1)

Application Number Title Priority Date Filing Date
EP81400023A Expired EP0032469B1 (fr) 1980-01-11 1981-01-09 Pipette à déplacement positif perfectionnée

Country Status (6)

Country Link
US (1) US4362064A (enrdf_load_stackoverflow)
EP (1) EP0032469B1 (enrdf_load_stackoverflow)
JP (1) JPS56104247A (enrdf_load_stackoverflow)
AT (1) ATE3248T1 (enrdf_load_stackoverflow)
DE (1) DE3160228D1 (enrdf_load_stackoverflow)
FR (1) FR2474340A1 (enrdf_load_stackoverflow)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4418580A (en) * 1981-10-13 1983-12-06 Sherwood Medical Company Pipettor mechanism and disposable tip and piston assembly
FR2515339A1 (fr) * 1981-10-28 1983-04-29 Marteau D Autry Eric Pipette de precision a deplacement positif
EP0197921A1 (en) * 1984-10-02 1986-10-22 DEVAUGHN, Donald H. Pipetting techniques using replaceable tips
US4672857A (en) * 1985-09-10 1987-06-16 Labindustries, Inc. Liquid microdispenser
US4824641A (en) * 1986-06-20 1989-04-25 Cetus Corporation Carousel and tip
US4748859A (en) * 1987-03-06 1988-06-07 Rainin Instrument Co., Inc. Disposable pipette tip
US4933148A (en) * 1988-08-04 1990-06-12 Brandeis University Pipetter barrel extension tube
FI86812C (fi) * 1991-01-07 1992-10-26 Labsystems Oy Foertraengningspipett
US5192511A (en) * 1991-05-31 1993-03-09 Tri-Continent Scientific, Inc. Pipette tip and piston
US6814936B1 (en) 1999-07-01 2004-11-09 Goran Enhorning Pipette assembly having a small volume disposable tip
FR2807345B1 (fr) * 2000-04-07 2002-07-12 Gilson Sa Pipette de prelevement a demontage simplifie
US6973845B2 (en) 2001-01-18 2005-12-13 Beckman Coulter, Inc. Low insertion force tip/mandrel
FR2874839B1 (fr) * 2004-09-07 2007-03-02 Gilson Sas Soc Par Actions Sim Pipette comprenant un equipage de commande et un equipage mobile a piston
US7976793B2 (en) * 2003-11-27 2011-07-12 Gilson S.A.S. Electronic pipette
US7641859B2 (en) * 2004-02-11 2010-01-05 Matrix Technologies Corporation Pipette tip mounting and ejection assembly and associated pipette tip
WO2005118129A1 (en) * 2004-05-27 2005-12-15 Stratos Biosystems, Llc Solid-phase affinity-based method for preparing and manipulating an analyte-containing solution
WO2006083695A2 (en) * 2005-01-28 2006-08-10 Parker-Hannifin Corporation Sampling probe, gripper and interface for laboratory sample management systems
US8192698B2 (en) * 2006-01-27 2012-06-05 Parker-Hannifin Corporation Sampling probe, gripper and interface for laboratory sample management systems
US8899118B1 (en) 2009-05-11 2014-12-02 Dan Seguin Multi-channel aspirating and dispensing instrument
FR2980123B1 (fr) * 2011-09-19 2013-10-11 Gilson Sas Pipette a deplacement positif presentant une fonction d'ejection amelioree
CN102847569A (zh) * 2012-08-29 2013-01-02 浙江硕华医用塑料有限公司 一次性移液管
FR3003643B1 (fr) * 2013-03-25 2015-04-17 Gilson Sas Systeme de pipetage a deplacement positif presentant une fonction d'ejection amelioree
FR3012883B1 (fr) * 2013-11-07 2015-12-25 Gilson Sas Systeme de pipetage a deplacement positif, presentant une conception facilitant la prehension du piston de l'ensemble capillaire-piston
CN104502150B (zh) * 2014-12-16 2017-01-04 武汉海王新能源工程技术有限公司 核电站用放射性液体样品取样装置及取样方法
EP3471878B1 (en) * 2016-06-15 2024-01-03 Hamilton Company Pipette tip
DE102016121813A1 (de) * 2016-11-14 2018-05-17 Ika-Werke Gmbh & Co. Kg Pipette und Verfahren zum Einstellen eines zu pipettierenden Volumens einer Pipette

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2324366A1 (fr) * 1975-09-18 1977-04-15 Labora Mannheim Gmbh Pipette de laboratoire perfectionnee
US4023716A (en) * 1976-04-20 1977-05-17 Justin Joel Shapiro Micro-dispensing liquid pipet

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2711124C2 (de) * 1977-03-15 1979-05-10 Labora Mannheim Gmbh Fuer Labortechnik, 6800 Mannheim Handpipette

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2324366A1 (fr) * 1975-09-18 1977-04-15 Labora Mannheim Gmbh Pipette de laboratoire perfectionnee
US4023716A (en) * 1976-04-20 1977-05-17 Justin Joel Shapiro Micro-dispensing liquid pipet

Also Published As

Publication number Publication date
US4362064A (en) 1982-12-07
DE3160228D1 (en) 1983-06-16
EP0032469A1 (fr) 1981-07-22
ATE3248T1 (de) 1983-05-15
FR2474340B1 (enrdf_load_stackoverflow) 1982-06-04
JPS6366577B2 (enrdf_load_stackoverflow) 1988-12-21
JPS56104247A (en) 1981-08-19
FR2474340A1 (fr) 1981-07-31

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