EA202091601A1 - SYSTEMS AND METHODS FOR IDENTIFICATION OF VIRAL CONTAMINANTS - Google Patents

SYSTEMS AND METHODS FOR IDENTIFICATION OF VIRAL CONTAMINANTS

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Publication number
EA202091601A1
EA202091601A1 EA202091601A EA202091601A EA202091601A1 EA 202091601 A1 EA202091601 A1 EA 202091601A1 EA 202091601 A EA202091601 A EA 202091601A EA 202091601 A EA202091601 A EA 202091601A EA 202091601 A1 EA202091601 A1 EA 202091601A1
Authority
EA
Eurasian Patent Office
Prior art keywords
viral
identification
systems
methods
contamination
Prior art date
Application number
EA202091601A
Other languages
Russian (ru)
Inventor
Дэвид Вэнхаут
Стивен Дэвис
Original Assignee
Ридженерон Фармасьютикалз, Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ридженерон Фармасьютикалз, Инк. filed Critical Ридженерон Фармасьютикалз, Инк.
Priority claimed from PCT/US2019/019355 external-priority patent/WO2019168774A1/en
Publication of EA202091601A1 publication Critical patent/EA202091601A1/en

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Abstract

Настоящее изобретение направлено на упрощенный процесс подготовки образцов, VERA (Viral Enrichment by Reducing Artifacts - вирусное обогащение путем уменьшения артефактов), чтобы обогатить общий геномный материал вирусной геномной ДНК/РНК. Это уменьшение геномных артефактов хозяина может быть осуществлено менее чем через 8 ч после взятия образца. Используя протокол быстрой подготовки библиотеки (~15 мин) и секвенирование с помощью нанопор в реальном времени, потенциальная вирусная контаминация, например контаминация вирусной РНК, может быть идентифицирована менее чем за один рабочий день с момента получения образца.The present invention is directed to a simplified sample preparation process, VERA (Viral Enrichment by Reducing Artifacts), to enrich the total genomic material of viral genomic DNA / RNA. This reduction in host genomic artifacts can be accomplished less than 8 hours after sampling. Using a rapid library preparation protocol (~ 15 min) and real-time nanopore sequencing, potential viral contamination, such as viral RNA contamination, can be identified in less than one business day from sample receipt.

EA202091601A 2018-08-31 2019-02-25 SYSTEMS AND METHODS FOR IDENTIFICATION OF VIRAL CONTAMINANTS EA202091601A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862725543P 2018-08-31 2018-08-31
PCT/US2019/019355 WO2019168774A1 (en) 2018-02-28 2019-02-25 Systems and methods for identifying viral contaminants

Publications (1)

Publication Number Publication Date
EA202091601A1 true EA202091601A1 (en) 2020-11-18

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ID=73649859

Family Applications (1)

Application Number Title Priority Date Filing Date
EA202091601A EA202091601A1 (en) 2018-08-31 2019-02-25 SYSTEMS AND METHODS FOR IDENTIFICATION OF VIRAL CONTAMINANTS

Country Status (1)

Country Link
EA (1) EA202091601A1 (en)

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