EA201800637A1 - METHOD FOR DETERMINING LEAD (II) IN AQUATIC ENVIRONMENTAL AND BIOLOGICAL SAMPLES - Google Patents

METHOD FOR DETERMINING LEAD (II) IN AQUATIC ENVIRONMENTAL AND BIOLOGICAL SAMPLES

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Publication number
EA201800637A1
EA201800637A1 EA201800637A EA201800637A EA201800637A1 EA 201800637 A1 EA201800637 A1 EA 201800637A1 EA 201800637 A EA201800637 A EA 201800637A EA 201800637 A EA201800637 A EA 201800637A EA 201800637 A1 EA201800637 A1 EA 201800637A1
Authority
EA
Eurasian Patent Office
Prior art keywords
lead
sensor film
polymer sensor
determination
color
Prior art date
Application number
EA201800637A
Other languages
Russian (ru)
Other versions
EA036346B1 (en
Inventor
Мария Анатольевна Пешкова
Андрей Владимирович Калиничев
Дарья Иосифовна Декина
Original Assignee
Федеральное государственное бюджетное образовательное учреждение высшего образования "Санкт-Петербургский государственный университет" (СПбГУ)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Федеральное государственное бюджетное образовательное учреждение высшего образования "Санкт-Петербургский государственный университет" (СПбГУ) filed Critical Федеральное государственное бюджетное образовательное учреждение высшего образования "Санкт-Петербургский государственный университет" (СПбГУ)
Publication of EA201800637A1 publication Critical patent/EA201800637A1/en
Publication of EA036346B1 publication Critical patent/EA036346B1/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
    • G01N31/22—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using chemical indicators
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/20—Metals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plasma & Fusion (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Изобретение представляет собой новый экспрессный оптический аналитический способ определения свинца(II) в водных объектах анализа: в питьевой воде, в природных водах, в промышленных сточных водах, а также определения патологических концентраций свинца(II) в биологических жидкостях. Сущность способа состоит в помещении полимерной сенсорной пленки в известный объем анализируемого раствора и после её извлечения в сравнении цвета полимерной сенсорной пленки с калиброванной цветовой шкалой. Полуколичественно содержание свинца(II) в испытуемом образце определяют по совпадению цвета полимерной сенсорной пленки с определённым делением калиброванной цветовой шкалы. Количественное содержание свинца(II) в испытуемом образце определяют спектрофотометрически, путём измерения поглощения полимерной сенсорной пленки с последующим определением содержания свинца(II) по градуировочному графику. В качестве сенсорной пленки используют пластифицированную бис-(этилгексиловым) эфиром себациновой кислоты поли(винилхлоридную) плёнку, содержащую 9-диметиламино-5-[4-(15-бутил-1,13-диоксо-2,14-иоксанонадецил)фенилимино]бензо[a]феноксазин 4-трет-бутил-каликс[4]арен-тетракис-(N,N-диметилтиоацетамид) и тетракис-[3,5-бис-(трифторметил)фенил]борат натрия. Способ может применяться для селективного скрининга групп лиц, находящихся в зоне повышенного риска отравления тяжелыми металлами, также возможен экспресс-анализ мочи и выявление степени отравления тяжелыми металлами при их утечках в окружающую среду в случае аварий, техногенных ЧП.The invention is a new express optical analytical method for the determination of lead (II) in water objects of analysis: in drinking water, in natural waters, in industrial wastewater, as well as for the determination of pathological concentrations of lead (II) in biological fluids. The essence of the method consists in placing a polymer sensor film in a known volume of the analyzed solution and, after removing it, comparing the color of the polymer sensor film with a calibrated color scale. The semi-quantitative content of lead (II) in the test sample is determined by the coincidence of the color of the polymer sensor film with a certain division of the calibrated color scale. The quantitative content of lead (II) in the test sample is determined spectrophotometrically by measuring the absorption of a polymer sensor film with subsequent determination of the content of lead (II) according to a calibration curve. As a sensor film, a poly (vinyl chloride) film plasticized with bis- (ethylhexyl) sebacic acid ester is used, containing 9-dimethylamino-5- [4- (15-butyl-1,13-dioxo-2,14-ioxanonadecyl) phenylimino] benzo [a] phenoxazine 4-tert-butyl-calix [4] arene-tetrakis- (N, N-dimethylthioacetamide) and tetrakis- [3,5-bis (trifluoromethyl) phenyl] sodium borate. The method can be used for selective screening of groups of people who are in the zone of increased risk of heavy metal poisoning; also, express urine analysis and detection of the degree of heavy metal poisoning during their leakage into the environment in case of accidents, man-made emergency situations are also possible.

EA201800637A 2018-08-16 2018-12-27 Method for determining lead(ii) in environmental water bodies and biological samples EA036346B1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2018129943A RU2682162C1 (en) 2018-08-16 2018-08-16 Method for determining lead (ii) in water and biological samples

Publications (2)

Publication Number Publication Date
EA201800637A1 true EA201800637A1 (en) 2020-02-28
EA036346B1 EA036346B1 (en) 2020-10-29

Family

ID=65806142

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201800637A EA036346B1 (en) 2018-08-16 2018-12-27 Method for determining lead(ii) in environmental water bodies and biological samples

Country Status (2)

Country Link
EA (1) EA036346B1 (en)
RU (1) RU2682162C1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2715478C1 (en) * 2019-05-16 2020-02-28 федеральное государственное автономное образовательное учреждение высшего образования "Национальный исследовательский университет ИТМО" (Университет ИТМО) Method of determining concentration of lead (ii) in aqueous samples
CN112945950A (en) * 2021-03-08 2021-06-11 唐山三友化工股份有限公司 Limit analysis method for enriching heavy metals in food additive sodium carbonate by using microporous filter membrane
WO2022267799A1 (en) * 2021-06-23 2022-12-29 中国石油化工股份有限公司 Water quality testing method and water quality testing apparatus
US11371978B1 (en) 2021-06-23 2022-06-28 Mks Vision, Llc System and method for detecting lead in water

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5217594A (en) * 1992-01-15 1993-06-08 Enzyme Technology Research Group, Inc. Convenient determination of trace lead in whole blood and other fluids
RU2218563C2 (en) * 1998-02-16 2003-12-10 Вашенков Евгений Георгиевич Technique and indicator for detection of lead, process of manufacture of indicator
US7247489B2 (en) * 2002-03-11 2007-07-24 Auburn University Ion-detecting microspheres and methods of use thereof
JP2005098760A (en) * 2003-09-22 2005-04-14 National Institute Of Advanced Industrial & Technology Lead ion selective coloring material, and lead ion simplified quantitative determining method using coloring material
CN101592597B (en) * 2009-06-25 2011-04-27 北京博晖创新光电技术股份有限公司 Matrix modifying reagent for detecting content of lead and cadmium elements in whole blood and application thereof
RU2441232C1 (en) * 2010-08-02 2012-01-27 Государственное образовательное учреждение высшего профессионального образования Уральский государственный лесотехнический университет Method of determining lead (ii) ions in solution
RU2529660C1 (en) * 2013-03-12 2014-09-27 Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Кубанский государственный университет" (ФГБОУ ВПО "КубГУ") Sorption-spectrophotometric method of determining lead (ii)

Also Published As

Publication number Publication date
EA036346B1 (en) 2020-10-29
RU2682162C1 (en) 2019-03-15

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MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ BY KZ KG TJ TM RU