EA201400641A1 - METHOD OF DIFFERENTIAL DIAGNOSTICS OF BALANCE DISORDERS OF VAGINAL MICROFLORA - Google Patents
METHOD OF DIFFERENTIAL DIAGNOSTICS OF BALANCE DISORDERS OF VAGINAL MICROFLORAInfo
- Publication number
- EA201400641A1 EA201400641A1 EA201400641A EA201400641A EA201400641A1 EA 201400641 A1 EA201400641 A1 EA 201400641A1 EA 201400641 A EA201400641 A EA 201400641A EA 201400641 A EA201400641 A EA 201400641A EA 201400641 A1 EA201400641 A1 EA 201400641A1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- dna
- concentration
- bacterial vaginosis
- microflora
- bacteria
- Prior art date
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Изобретение относится к медицине, более конкретно к лабораторной диагностике и может быть использовано для дифференциальной диагностики нарушений баланса вагинальной микрофлоры. У пациентки берут влагалищный мазок и определяют в нём концентрацию ДНК всех бактерий, концентрацию ДНК бактерии Gardnerella vaginalis, концентрацию ДНК бактерии Atopobium vaginae и концентрацию ДНК бактерий Lactobacillus spp. методом амплификации нуклеиновых кислот. На основании полученных данных рассчитывают коэффициенты соотношений концентраций: КС1 = lg [ДНК Lac] - lg ([ДНК Gv] + [ДНК Av]), КС2 = lg [ДНК Вас] - lg [ДНК Lac] и КС3 = lg [ДНК Вас] - lg ([ДНК Gv] + [ДНК Av]). При lg [ДНК Вас]≥5, КС1>1 и КС2≤1 констатируют отсутствие бактериального вагиноза, при lg [ДНК Вас]≥5, 0.5≤КС1≤1, КС2≤1 и КС3≤2 констатируют промежуточное состояние микрофлоры, при lg [ДНК Вас]≥5, КС1<0.5 и КС3≤2 диагностируют бактериальный вагиноз, а при lg [ДНК Вас]<5 констатируют, что количество бактерий недостаточно для анализа. При отсутствии бактериального вагиноза и 5≤lg [ДНК Вас]<6 констатируют снижение степени бактериальной обсеменённости влагалища. Дополнительно в мазке определяют концентрацию ДНК Enterobacteriaceae, концентрацию ДНК бактерий Staphylococcus spp. и концентрацию ДНК бактерий Streptococcus spp. методом амплификации нуклеиновых кислот. При lg [ДНК Вас]≥6, ([ДНК Ent] + [ДНК Staph] + [ДНК Strep]) ≤ [ДНК Lac] и отсутствии бактериального вагиноза делают заключение "нарушений баланса вагинальной микрофлоры не выявлено", при lg ([ДНК Gv] + [ДНК Av]) - lg ([ДНК Ent] + [ДНК Staph] + [ДНК Strep])≤1 диагноз бактериальный вагиноз уточняют как бактериальный вагиноз в сочетании с повышенным содержанием аэробной микрофлоры, а при lg [ДНК Вас]≥5, ([ДНК Ent] + [ДНК Staph] + [ДНК Strep]) > [ДНК Lac], КС2>1 и КС3>2 констатируют преобладание аэробной микрофлоры. При наличии местной воспалительной реакции в сочетании с повышенным содержанием или преобладанием аэробнойThe invention relates to medicine, more specifically to laboratory diagnosis and can be used for the differential diagnosis of imbalance of the vaginal microflora. A vaginal swab is taken from the patient and the DNA concentration of all bacteria, the DNA concentration of the Gardnerella vaginalis bacterium, the DNA concentration of the Atopobium vaginae bacterium and the DNA concentration of Lactobacillus spp bacteria are determined in it. nucleic acid amplification method. Based on the data obtained, the ratios of the concentrations of concentrations are calculated: KC1 = lg [DNA Lac] - lg ([DNA Gv] + [DNA Av]), KC2 = lg [DNA You] - lg [DNA Lac] and KC3 = lg [DNA You ] - lg ([DNA Gv] + [DNA Av]). With lg [DNA of You] ≥5, KC1> 1 and KS2≤1 state the absence of bacterial vaginosis, with lg [DNA of You] ≥5, 0.5≤KS1≤1, KS2≤1 and KS3≤2 state the intermediate state of microflora, with lg [DNA of you] ≥5, KC1 <0.5 and KC3≤2 diagnose bacterial vaginosis, and with lg [DNA of You] <5, it is ascertained that the number of bacteria is not enough for analysis. In the absence of bacterial vaginosis and 5≤lg [DNA of you] <6, a decrease in the degree of bacterial contamination of the vagina is noted. Additionally, in a smear, the concentration of DNA Enterobacteriaceae, the DNA concentration of bacteria Staphylococcus spp. and DNA concentration of bacteria Streptococcus spp. nucleic acid amplification method. With lg [DNA of You] ≥6, ([DNA Ent] + [DNA of Staph] + [DNA Strep]) ≤ [DNA of Lac] and in the absence of bacterial vaginosis they conclude "no imbalance of the vaginal microflora was detected", with lg ([ Gv] + [DNA Av]) - lg ([DNA Ent] + [DNA Staph] + [DNA Strep]) ≤1 specify the diagnosis of bacterial vaginosis as bacterial vaginosis in combination with an increased content of aerobic microflora, and with lg [DNA of You] ≥5, ([DNA Ent] + [DNA Staph] + [DNA Strep])> [DNA Lac], КС2> 1 and КС3> 2 state the prevalence of aerobic microflora. In the presence of a local inflammatory reaction in combination with an increased content or predominance of aerobic
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EA201400641A EA025042B1 (en) | 2014-06-03 | 2014-06-03 | Method for differential diagnostics of vaginal microflora balance disorders |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EA201400641A EA025042B1 (en) | 2014-06-03 | 2014-06-03 | Method for differential diagnostics of vaginal microflora balance disorders |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EA201400641A1 true EA201400641A1 (en) | 2016-01-29 |
| EA025042B1 EA025042B1 (en) | 2016-11-30 |
Family
ID=55174374
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EA201400641A EA025042B1 (en) | 2014-06-03 | 2014-06-03 | Method for differential diagnostics of vaginal microflora balance disorders |
Country Status (1)
| Country | Link |
|---|---|
| EA (1) | EA025042B1 (en) |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2909099B1 (en) * | 2006-11-24 | 2012-10-19 | Univ Aix Marseille Ii | METHOD FOR DIAGNOSING AND MONITORING BACTERIAL VAGINOSIS BY MOLECULAR QUANTIFICATION |
| WO2011068679A1 (en) * | 2009-12-03 | 2011-06-09 | Quest Diagnostics Investments Incorporated | Methods for the diagnosis of bacterial vaginosis |
-
2014
- 2014-06-03 EA EA201400641A patent/EA025042B1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EA025042B1 (en) | 2016-11-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Jalanka et al. | Long-term effects on luminal and mucosal microbiota and commonly acquired taxa in faecal microbiota transplantation for recurrent Clostridium difficile infection | |
| Tsuji et al. | Molecular monitoring of the development of intestinal microbiota in Japanese infants | |
| US20230416671A1 (en) | Composition And Method For Stabilizing And Maintaining The Viability Of Hardy Microorganisms | |
| Hosseinzadeh et al. | Emerge of bla NDM-1 and bla OXA-48-like harboring carbapenem-resistant Klebsiella pneumoniae isolates from hospitalized patients in southwestern Iran | |
| RU2717644C2 (en) | Method, composition and kit for stabilizing deoxyribonucleic acids in biological samples | |
| Guiral et al. | Prevalence of Escherichia coli among samples collected from the genital tract in pregnant and nonpregnant women: relationship with virulence | |
| WO2017172644A3 (en) | Bacteria identification and antibiotic susceptibility profiling device | |
| Gergova et al. | Gardnerella vaginalis-associated bacterial vaginosis in Bulgarian women | |
| Habeeb et al. | Occurrence of virulence genes, 16S rRNA methylases, and plasmid-mediated quinolone resistance genes in CTX-M-producing Escherichia coli from Pakistan | |
| Treven et al. | Evaluation of human milk microbiota by 16S rRNA gene next-generation sequencing (NGS) and cultivation/MALDI-TOF mass spectrometry identification | |
| Jiang et al. | Disseminated nocardiosis caused by Nocardia otitidiscaviarum in an immunocompetent host: a case report and literature review | |
| Yılmaz et al. | Phylogenetic group/subgroups distributions, virulence factors, and antimicrobial susceptibility of Escherichia coli strains from urinary tract infections in Hatay | |
| Mashayekhi et al. | Molecular characterization and antimicrobial resistance of uropathogenic Escherichia coli | |
| Pires et al. | Clostridium difficile infection in Brazil: a neglected problem? | |
| EA201400641A1 (en) | METHOD OF DIFFERENTIAL DIAGNOSTICS OF BALANCE DISORDERS OF VAGINAL MICROFLORA | |
| Nally et al. | Identification of Burkholderia fungorum in the urine of an individual with spinal cord injury and augmentation cystoplasty using 16S sequencing: copathogen or innocent bystander? | |
| Girault et al. | Isolation of Leptospira from blood culture bottles | |
| Eshraghi et al. | Pulmonary nocardiosis associated with cerebral abscess successfully treated by co-trimoxazole: a case report | |
| Wang et al. | Prospective observational case series evaluating middle ear fluid and tympanostomy tubes through pyrosequencing | |
| Cereija et al. | Influence of anaerobic conditions on vaginal microbiota recovery from bacterial vaginosis patients | |
| Peretz et al. | BACTEC™ FX system as a tool for culturing gastric biopsies and Helicobacter pylori diagnosis | |
| Bates et al. | Achromobacter xylosoxidans in idiopathic cystic bronchiectasis | |
| Reddy et al. | Mcr-1 expression in progression of colistin resistance gram negative bacilli of clinical specimens derived from Intensive Care Units, wards and hospital setting of Deccan Eco Region of Southern India | |
| Lainhart et al. | New bugs and new drugs: updates in clinical microbiology | |
| Ma et al. | O05. 4 Association Between Chlamydia Trachomatis Genital Infection and the Vaginal Microbiome |