EA201400374A1 - A METHOD OF SEROLOGICAL DIAGNOSTICS OF A NICORATIVE MICROSPORIUM - Google Patents
A METHOD OF SEROLOGICAL DIAGNOSTICS OF A NICORATIVE MICROSPORIUMInfo
- Publication number
- EA201400374A1 EA201400374A1 EA201400374A EA201400374A EA201400374A1 EA 201400374 A1 EA201400374 A1 EA 201400374A1 EA 201400374 A EA201400374 A EA 201400374A EA 201400374 A EA201400374 A EA 201400374A EA 201400374 A1 EA201400374 A1 EA 201400374A1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- elisa
- microsporia
- antigen
- diagnosis
- average
- Prior art date
Links
Abstract
Изобретение относится к ветеринарии, а именно к диагностике инфекционных болезней животных. Технической задачей способа является разработка ИФА для совершенствования диагностики микроспории плотоядных, которая достигается тем, что при постановке реакции в качестве антигена для сенсибилизации планшета используют антиген гриба Microsporum canis №13, а выявление в сыворотке крови кошек и собак специфичных к названному антигену антител класса IgG, образование которых характерно для микроспории, осуществляют с помощью меченных ферментом иммуноглобулинов кролика против видового анти-IgG. Результаты ИФА проявляют хромогеном и оценивают спектрофотометрически или визуально. Положительными считают пробы сывороток крови, средняя величина ОП которых не менее чем в 2 раза превосходит среднюю ОП отрицательного контроля. Процент выявления больных животных по клиническим признакам составил 30%, в РМА - 74%, в предлагаемом ИФА - 88%. Титры антител иммунизированных животных равны 1:100-1:800, у больных кошек - 1:200-1:6400, у больных собак - 1:800-1:3200. Использование предлагаемого варианта ИФА позволит повысить качество диагностики микроспории.The invention relates to veterinary medicine, in particular to the diagnosis of infectious animal diseases. The technical task of the method is the development of ELISA to improve the diagnosis of microsporic carnivorous, which is achieved by setting the reaction as an antigen to sensitize the tablet using the antigen of the fungus Microsporum canis No. 13, and the detection in the serum of cats and dogs of IgG class-specific antigens, the formation of which is characteristic of microsporia, is carried out using rabbit immunoglobulins labeled with an enzyme against specific anti-IgG. The ELISA results are shown by the chromogen and evaluated spectrophotometrically or visually. Samples of blood sera are considered positive, the average OD of which is not less than 2 times higher than the average OD of the negative control. Clinically, the percentage of detection of diseased animals was 30%, 74% in PMA, and 88% in the proposed ELISA. The antibody titers of immunized animals are 1: 100-1: 800, in sick cats - 1: 200-1: 6400, in sick dogs - 1: 800-1: 3200. The use of the proposed ELISA option will improve the quality of diagnostics of microsporia.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KZ20131797 | 2013-11-29 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EA201400374A1 true EA201400374A1 (en) | 2015-06-30 |
| EA029205B1 EA029205B1 (en) | 2018-02-28 |
Family
ID=53488037
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EA201400374A EA029205B1 (en) | 2013-11-29 | 2013-12-27 | Method for serological diagnostics of microsporia in carnivorous animals |
Country Status (1)
| Country | Link |
|---|---|
| EA (1) | EA029205B1 (en) |
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2013
- 2013-12-27 EA EA201400374A patent/EA029205B1/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| EA029205B1 (en) | 2018-02-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s) |
Designated state(s): AM AZ BY KZ KG TJ TM RU |