EA201290612A1 - A METHOD FOR ISOLATING NUCLEIC ACID AND A SET FOR THIS - Google Patents

A METHOD FOR ISOLATING NUCLEIC ACID AND A SET FOR THIS

Info

Publication number
EA201290612A1
EA201290612A1 EA201290612A EA201290612A EA201290612A1 EA 201290612 A1 EA201290612 A1 EA 201290612A1 EA 201290612 A EA201290612 A EA 201290612A EA 201290612 A EA201290612 A EA 201290612A EA 201290612 A1 EA201290612 A1 EA 201290612A1
Authority
EA
Eurasian Patent Office
Prior art keywords
nucleic acids
cotton wool
cotton
acid
bind
Prior art date
Application number
EA201290612A
Other languages
Russian (ru)
Other versions
EA022842B1 (en
Inventor
Пхани Кумар Пуллела
Мулаккапуратх Нараянан Маной
Сантош Кумар Гандхавалла
Митчелл Притхэм Пинто
Чандрасекхар Бхаскаран Наир
Пилларисетти Венката Суббарао
Original Assignee
Бигтек Прайвит Лимитед
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Бигтек Прайвит Лимитед filed Critical Бигтек Прайвит Лимитед
Publication of EA201290612A1 publication Critical patent/EA201290612A1/en
Publication of EA022842B1 publication Critical patent/EA022842B1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C07H1/08—Separation; Purification from natural products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Согласно настоящему изобретению предложен способ выделения природных и синтетических нуклеиновых кислот, таких как дезоксирибонуклеиновая кислота (ДНК), рибонуклеиновая кислота (РНК) и пептидо-нуклеиновая кислота (ПНК), из твердого или жидкого образца с использованием ваты. Вата упакована таким образом, что раствор, содержащий нуклеиновые кислоты, проходит через нее, и нуклеиновые кислоты из указанного раствора связываются с указанной ватой в среде, оптимальной для связывания. Нуклеиновые кислоты связываются с ватой таким образом, что связанные нуклеиновые кислоты могут выдержать многочисленные промывки жидкостью, содержащей воду, и вымываться в водном буфере, с которым вымывающиеся нуклеиновые кислоты могут непосредственно использоваться для амплификации с помощью ПЦР или для любых других биохимических или молукулярно-биологических целей.The present invention provides a method for isolating natural and synthetic nucleic acids, such as deoxyribonucleic acid (DNA), ribonucleic acid (RNA), and peptide nucleic acid (PNA), from a solid or liquid sample using cotton wool. Cotton wool is packaged in such a way that a solution containing nucleic acids passes through it, and nucleic acids from said solution bind to said cotton wool in an optimal binding medium. Nucleic acids bind to cotton so that bound nucleic acids can withstand multiple washes with a liquid containing water and washed in an aqueous buffer with which leaching nucleic acids can be directly used for amplification by PCR or for any other biochemical or molecular biological purposes .

EA201290612A 2010-01-07 2011-01-06 Method for isolation of nucleic acids and a kit thereof EA022842B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN50CH2010 2010-01-07
PCT/IB2011/050044 WO2011083429A1 (en) 2010-01-07 2011-01-06 A method for isolation of nucleic acids and a kit thereof

Publications (2)

Publication Number Publication Date
EA201290612A1 true EA201290612A1 (en) 2013-02-28
EA022842B1 EA022842B1 (en) 2016-03-31

Family

ID=44305232

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201290612A EA022842B1 (en) 2010-01-07 2011-01-06 Method for isolation of nucleic acids and a kit thereof

Country Status (21)

Country Link
US (1) US8986976B2 (en)
EP (1) EP2521780B1 (en)
JP (1) JP5791631B2 (en)
KR (1) KR101590544B1 (en)
CN (1) CN102725407B (en)
BR (1) BR112012016774B1 (en)
CY (1) CY1119878T1 (en)
DK (1) DK2521780T3 (en)
EA (1) EA022842B1 (en)
ES (1) ES2652335T3 (en)
HR (1) HRP20171823T1 (en)
HU (1) HUE034870T2 (en)
LT (1) LT2521780T (en)
NO (1) NO2521780T3 (en)
PL (1) PL2521780T3 (en)
PT (1) PT2521780T (en)
RS (1) RS56636B1 (en)
SG (1) SG182372A1 (en)
SI (1) SI2521780T1 (en)
WO (1) WO2011083429A1 (en)
ZA (1) ZA201205053B (en)

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CN103882006A (en) * 2012-12-19 2014-06-25 河北省健海生物芯片技术有限责任公司 Preparation method and extraction method for DNA extraction reagent suitable for various samples
CN103289990A (en) * 2013-06-14 2013-09-11 浙江世纪康大医疗科技有限公司 Rapid extraction method of blood deoxyribonucleic acid (DNA)
KR102214211B1 (en) * 2013-08-16 2021-02-10 글로벌 라이프 사이언시스 솔루션즈 오퍼레이션스 유케이 엘티디 Methods and compositions for extraction and storage of nucleic acids
TWI642679B (en) * 2014-07-29 2018-12-01 精專生醫股份有限公司 Device for purifying nucleic acid molecules and method using same
CN105734044A (en) * 2014-12-08 2016-07-06 苏州新波生物技术有限公司 Rinsing liquid for nucleic acid extraction purification
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JP2019506615A (en) * 2016-02-23 2019-03-07 ビッグテック プライベート リミテッドＢｉｇｔｅｃ Ｐｒｉｖａｔｅ Ｌｉｍｉｔｅｄ Cartridge for sample purification and analysis
RU2628695C1 (en) * 2016-06-14 2017-08-21 Общество с ограниченной ответственностью "Биологическая среда" Method for rna and dna isolation from dry biological samples stored on paper media and set for its implementation
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RU2651937C1 (en) * 2017-05-04 2018-04-24 Татьяна Георгиевна Фалеева Composition for collection and storage of dna or dna-containing biological traces (variants) and their application
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CN109385418B (en) * 2017-08-03 2022-07-22 杭州优思达生物技术有限公司 Method and reagent for extracting virus/bacterium nucleic acid in animal sample
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CN110029105A (en) * 2019-05-14 2019-07-19 成都罗宁生物科技有限公司 A kind of kit and its method extracting microbial DNA
CN113025608A (en) * 2019-12-09 2021-06-25 深圳市真迈生物科技有限公司 Cell lysate, kit and application
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Also Published As

Publication number Publication date
RS56636B1 (en) 2018-03-30
KR101590544B1 (en) 2016-02-18
KR20120104625A (en) 2012-09-21
HUE034870T2 (en) 2018-03-28
JP5791631B2 (en) 2015-10-07
CN102725407B (en) 2016-09-14
NO2521780T3 (en) 2018-01-20
ZA201205053B (en) 2013-03-27
EP2521780A4 (en) 2013-07-31
LT2521780T (en) 2018-02-12
HRP20171823T1 (en) 2018-01-12
US20130203150A1 (en) 2013-08-08
WO2011083429A1 (en) 2011-07-14
JP2013516186A (en) 2013-05-13
DK2521780T3 (en) 2017-12-04
PL2521780T3 (en) 2018-03-30
EP2521780B1 (en) 2017-08-23
BR112012016774B1 (en) 2020-12-01
ES2652335T3 (en) 2018-02-01
SG182372A1 (en) 2012-08-30
US8986976B2 (en) 2015-03-24
BR112012016774A2 (en) 2019-09-10
EP2521780A1 (en) 2012-11-14
SI2521780T1 (en) 2018-02-28
PT2521780T (en) 2017-12-21
CY1119878T1 (en) 2018-06-27
CN102725407A (en) 2012-10-10
EA022842B1 (en) 2016-03-31

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