EA200600709A1 - METHOD OF STORAGE OF SPERMATOSIS AND PRODUCT FOR ARTIFICIAL IMPLEMENTATION (OPTIONS) - Google Patents
METHOD OF STORAGE OF SPERMATOSIS AND PRODUCT FOR ARTIFICIAL IMPLEMENTATION (OPTIONS)Info
- Publication number
- EA200600709A1 EA200600709A1 EA200600709A EA200600709A EA200600709A1 EA 200600709 A1 EA200600709 A1 EA 200600709A1 EA 200600709 A EA200600709 A EA 200600709A EA 200600709 A EA200600709 A EA 200600709A EA 200600709 A1 EA200600709 A1 EA 200600709A1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- injection
- spermatozoa
- agarose gel
- hollow microsphere
- sperm
- Prior art date
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Изобретение относится к медицине и ветеринарии, в частности, к эмбриологии и может быть использовано для хранения микроскопических биологических объектов. Способ хранения сперматозоидов характеризуется тем, что по меньшей мере один сперматозоид (6) вводят путем инъекции в полую микросферу из агарозного геля (5), находящуюся в инкубационной среде. Продукт для искусственного оплодотворения in vitro содержит по меньшей мере одну полую микросферу из агарозного геля, заполненную инкубационной средой, в которой путем инъекции размещен по меньшей мере один сперматозоид. Продукт для искусственного оплодотворения содержит подвергнутую криоконсервации по меньшей мере одну полую микросферу из агарозного геля, заполненную раствором по меньшей мере одного криопротектора, в которой путем инъекции размещен по меньшей мере один сперматозоид. Технический результат заключается в обеспечении возможности сохранения единичных сперматозоидов. Технический результат заключается в повышении сохранения жизнеспособности сперматозоидов и их функции, в повышении нормальной морфологии и сохранения подвижности сперматозоидов после периода хранения, в улучшении стерильности процесса, что предотвращает возможность инфицирования сперматозоидов.The invention relates to medicine and veterinary medicine, in particular, to embryology and can be used to store microscopic biological objects. The method of storing spermatozoa is characterized by the fact that at least one spermatozoon (6) is introduced by injection into a hollow microsphere from an agarose gel (5) that is in an incubation medium. An in vitro in vitro fertilization product contains at least one hollow microsphere from an agarose gel, filled with an incubation medium in which at least one spermatozoon is placed by injection. The artificial insemination product contains cryopreservation of at least one hollow microsphere from an agarose gel, filled with a solution of at least one cryoprotectant, in which at least one sperm cell is placed by injection. The technical result is to provide the ability to save single sperm. The technical result is to increase the preservation of the viability of spermatozoa and their function, to increase the normal morphology and preserve the motility of spermatozoa after a period of storage, to improve the sterility of the process, which prevents the possibility of infection of sperm.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EA200600709A EA008674B1 (en) | 2005-12-29 | 2005-12-29 | Method for storing spermatozoa and product for telegenesis |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EA200600709A EA008674B1 (en) | 2005-12-29 | 2005-12-29 | Method for storing spermatozoa and product for telegenesis |
Publications (2)
Publication Number | Publication Date |
---|---|
EA200600709A1 true EA200600709A1 (en) | 2007-06-29 |
EA008674B1 EA008674B1 (en) | 2007-06-29 |
Family
ID=41263890
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EA200600709A EA008674B1 (en) | 2005-12-29 | 2005-12-29 | Method for storing spermatozoa and product for telegenesis |
Country Status (1)
Country | Link |
---|---|
EA (1) | EA008674B1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110367243A (en) * | 2019-08-07 | 2019-10-25 | 中国人民解放军第四军医大学 | A kind of hollow agar sugar ball and preparation method for monosperm freezen protective |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
RU2504581C2 (en) * | 2012-02-02 | 2014-01-20 | Константин Владимирович Кириенко | Method for vital immobilisation of human and animal sperm cells |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2718369B1 (en) * | 1994-04-12 | 1996-06-21 | Lipogel Sarl | Gelled microspheres, their preparation process and their applications. |
US6132952A (en) * | 1997-05-30 | 2000-10-17 | Saint Barnabas Medical Center | Storage system comprising an emptied zona pellucida and spermatozoa placed therein and a method of cryopreservation |
US6641526B1 (en) * | 1998-03-20 | 2003-11-04 | University Of Hawaii | Development of normal offspring from oocytes injected with freeze-dried spermatozoa |
RU2254101C2 (en) * | 2001-08-01 | 2005-06-20 | Федеральное государственное унитарное предприятие "Башкирское" по племенной работе | Method for preparing for spermatic cryoconservation in farm animals |
-
2005
- 2005-12-29 EA EA200600709A patent/EA008674B1/en not_active IP Right Cessation
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110367243A (en) * | 2019-08-07 | 2019-10-25 | 中国人民解放军第四军医大学 | A kind of hollow agar sugar ball and preparation method for monosperm freezen protective |
Also Published As
Publication number | Publication date |
---|---|
EA008674B1 (en) | 2007-06-29 |
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Legal Events
Date | Code | Title | Description |
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MM4A | Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s) |
Designated state(s): AM AZ BY KZ KG MD TJ TM |
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MM4A | Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s) |
Designated state(s): RU |