EA200600709A1 - METHOD OF STORAGE OF SPERMATOSIS AND PRODUCT FOR ARTIFICIAL IMPLEMENTATION (OPTIONS) - Google Patents

METHOD OF STORAGE OF SPERMATOSIS AND PRODUCT FOR ARTIFICIAL IMPLEMENTATION (OPTIONS)

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Publication number
EA200600709A1
EA200600709A1 EA200600709A EA200600709A EA200600709A1 EA 200600709 A1 EA200600709 A1 EA 200600709A1 EA 200600709 A EA200600709 A EA 200600709A EA 200600709 A EA200600709 A EA 200600709A EA 200600709 A1 EA200600709 A1 EA 200600709A1
Authority
EA
Eurasian Patent Office
Prior art keywords
injection
spermatozoa
agarose gel
hollow microsphere
sperm
Prior art date
Application number
EA200600709A
Other languages
Russian (ru)
Other versions
EA008674B1 (en
Inventor
Дмитрий Александрович Исаев
Сергей Юрьевич Залетов
Original Assignee
Дмитрий Александрович Исаев
Сергей Юрьевич Залетов
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Дмитрий Александрович Исаев, Сергей Юрьевич Залетов filed Critical Дмитрий Александрович Исаев
Priority to EA200600709A priority Critical patent/EA008674B1/en
Publication of EA200600709A1 publication Critical patent/EA200600709A1/en
Publication of EA008674B1 publication Critical patent/EA008674B1/en

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Изобретение относится к медицине и ветеринарии, в частности, к эмбриологии и может быть использовано для хранения микроскопических биологических объектов. Способ хранения сперматозоидов характеризуется тем, что по меньшей мере один сперматозоид (6) вводят путем инъекции в полую микросферу из агарозного геля (5), находящуюся в инкубационной среде. Продукт для искусственного оплодотворения in vitro содержит по меньшей мере одну полую микросферу из агарозного геля, заполненную инкубационной средой, в которой путем инъекции размещен по меньшей мере один сперматозоид. Продукт для искусственного оплодотворения содержит подвергнутую криоконсервации по меньшей мере одну полую микросферу из агарозного геля, заполненную раствором по меньшей мере одного криопротектора, в которой путем инъекции размещен по меньшей мере один сперматозоид. Технический результат заключается в обеспечении возможности сохранения единичных сперматозоидов. Технический результат заключается в повышении сохранения жизнеспособности сперматозоидов и их функции, в повышении нормальной морфологии и сохранения подвижности сперматозоидов после периода хранения, в улучшении стерильности процесса, что предотвращает возможность инфицирования сперматозоидов.The invention relates to medicine and veterinary medicine, in particular, to embryology and can be used to store microscopic biological objects. The method of storing spermatozoa is characterized by the fact that at least one spermatozoon (6) is introduced by injection into a hollow microsphere from an agarose gel (5) that is in an incubation medium. An in vitro in vitro fertilization product contains at least one hollow microsphere from an agarose gel, filled with an incubation medium in which at least one spermatozoon is placed by injection. The artificial insemination product contains cryopreservation of at least one hollow microsphere from an agarose gel, filled with a solution of at least one cryoprotectant, in which at least one sperm cell is placed by injection. The technical result is to provide the ability to save single sperm. The technical result is to increase the preservation of the viability of spermatozoa and their function, to increase the normal morphology and preserve the motility of spermatozoa after a period of storage, to improve the sterility of the process, which prevents the possibility of infection of sperm.

EA200600709A 2005-12-29 2005-12-29 Method for storing spermatozoa and product for telegenesis EA008674B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EA200600709A EA008674B1 (en) 2005-12-29 2005-12-29 Method for storing spermatozoa and product for telegenesis

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EA200600709A EA008674B1 (en) 2005-12-29 2005-12-29 Method for storing spermatozoa and product for telegenesis

Publications (2)

Publication Number Publication Date
EA200600709A1 true EA200600709A1 (en) 2007-06-29
EA008674B1 EA008674B1 (en) 2007-06-29

Family

ID=41263890

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200600709A EA008674B1 (en) 2005-12-29 2005-12-29 Method for storing spermatozoa and product for telegenesis

Country Status (1)

Country Link
EA (1) EA008674B1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110367243A (en) * 2019-08-07 2019-10-25 中国人民解放军第四军医大学 A kind of hollow agar sugar ball and preparation method for monosperm freezen protective

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2504581C2 (en) * 2012-02-02 2014-01-20 Константин Владимирович Кириенко Method for vital immobilisation of human and animal sperm cells

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2718369B1 (en) * 1994-04-12 1996-06-21 Lipogel Sarl Gelled microspheres, their preparation process and their applications.
US6132952A (en) * 1997-05-30 2000-10-17 Saint Barnabas Medical Center Storage system comprising an emptied zona pellucida and spermatozoa placed therein and a method of cryopreservation
US6641526B1 (en) * 1998-03-20 2003-11-04 University Of Hawaii Development of normal offspring from oocytes injected with freeze-dried spermatozoa
RU2254101C2 (en) * 2001-08-01 2005-06-20 Федеральное государственное унитарное предприятие "Башкирское" по племенной работе Method for preparing for spermatic cryoconservation in farm animals

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110367243A (en) * 2019-08-07 2019-10-25 中国人民解放军第四军医大学 A kind of hollow agar sugar ball and preparation method for monosperm freezen protective

Also Published As

Publication number Publication date
EA008674B1 (en) 2007-06-29

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Legal Events

Date Code Title Description
MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ BY KZ KG MD TJ TM

MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): RU