EA200600708A1 - METHOD FOR OBTAINING THE EMPTY MICROSPHERES AND GETTING THE MICROSPHERE OBTAINED BY THIS METHOD - Google Patents

METHOD FOR OBTAINING THE EMPTY MICROSPHERES AND GETTING THE MICROSPHERE OBTAINED BY THIS METHOD

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Publication number
EA200600708A1
EA200600708A1 EA200600708A EA200600708A EA200600708A1 EA 200600708 A1 EA200600708 A1 EA 200600708A1 EA 200600708 A EA200600708 A EA 200600708A EA 200600708 A EA200600708 A EA 200600708A EA 200600708 A1 EA200600708 A1 EA 200600708A1
Authority
EA
Eurasian Patent Office
Prior art keywords
obtaining
hollow microspheres
microscopic
technical result
microspheres
Prior art date
Application number
EA200600708A
Other languages
Russian (ru)
Other versions
EA007992B1 (en
Inventor
Дмитрий Александрович Исаев
Сергей Юрьевич Залетов
Original Assignee
Дмитрий Александрович Исаев
Сергей Юрьевич Залетов
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Дмитрий Александрович Исаев, Сергей Юрьевич Залетов filed Critical Дмитрий Александрович Исаев
Priority to EA200600708A priority Critical patent/EA200600708A1/en
Publication of EA007992B1 publication Critical patent/EA007992B1/en
Publication of EA200600708A1 publication Critical patent/EA200600708A1/en

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Изобретение относится к биотехнологии и может быть использовано для получения полых микросфер, предназначенных для размещения в них микроскопических биологических объектов, таких как, сперматозоиды млекопитающих, ооциты или клетки организмов. Способ получения полых микросфер характеризуется тем, что над водной средой (3) осуществляют распыление водного раствора расплавленной агарозы (1) с образованием аэрозоля (4) в газовой среде при температуре ниже температуры застудневания водного раствора агарозы, с последующим перемещением образовавшихся микросфер в водную среду (3). Получаемые полые микросферы из агарозного геля готовы к размещению в них микроскопических биологических объектов. Технический результат заключается в реализации расширения арсенала технических средств для локализации и хранения микроскопических биологических объектов. Технический результат заключается в упрощении способа получения полых микросфер, улучшении стерильности процесса, получении нового устройства для локализации и хранения биологических микроскопических объектов из биохимически инертного и нетоксичного материала. Технически результат заключается также в повышении прозрачности, однородности и упругости стенки полой микросферы.The invention relates to biotechnology and can be used to obtain hollow microspheres designed to accommodate microscopic biological objects, such as mammalian spermatozoa, oocytes, or cells of organisms. The method of obtaining hollow microspheres is characterized by spraying an aqueous solution of molten agarose (1) above the aqueous medium (3) with the formation of an aerosol (4) in a gaseous medium at a temperature below the freezing point of an aqueous solution of agarose, followed by transferring the formed microspheres into the aqueous medium ( 3). The resulting hollow microspheres from agarose gel are ready for placement of microscopic biological objects in them. The technical result consists in the implementation of the expansion of the arsenal of technical means for the localization and storage of microscopic biological objects. The technical result consists in simplifying the method of obtaining hollow microspheres, improving the sterility of the process, obtaining a new device for localizing and storing biological microscopic objects from biochemically inert and non-toxic material. The technical result is also to increase the transparency, uniformity and elasticity of the wall of the hollow microsphere.

EA200600708A 2005-12-29 2005-12-29 METHOD FOR OBTAINING THE EMPTY MICROSPHERES AND GETTING THE MICROSPHERE OBTAINED BY THIS METHOD EA200600708A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EA200600708A EA200600708A1 (en) 2005-12-29 2005-12-29 METHOD FOR OBTAINING THE EMPTY MICROSPHERES AND GETTING THE MICROSPHERE OBTAINED BY THIS METHOD

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EA200600708A EA200600708A1 (en) 2005-12-29 2005-12-29 METHOD FOR OBTAINING THE EMPTY MICROSPHERES AND GETTING THE MICROSPHERE OBTAINED BY THIS METHOD

Publications (2)

Publication Number Publication Date
EA007992B1 EA007992B1 (en) 2007-02-27
EA200600708A1 true EA200600708A1 (en) 2007-02-27

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
EA200600708A EA200600708A1 (en) 2005-12-29 2005-12-29 METHOD FOR OBTAINING THE EMPTY MICROSPHERES AND GETTING THE MICROSPHERE OBTAINED BY THIS METHOD

Country Status (1)

Country Link
EA (1) EA200600708A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11254796B2 (en) * 2016-10-19 2022-02-22 Interfacial Consultants Llc Sacrificial microspheres

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1355212A1 (en) * 1984-06-22 1987-11-30 Институт проблем криобиологии и криомедицины АН УССР Method of preserving manъs sperm
US6596310B1 (en) * 2000-08-23 2003-07-22 Board Of Trustees Operating Michigan State University Method of artificial insemination by timed release of sperm from capsules or solid beads

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Publication number Publication date
EA007992B1 (en) 2007-02-27

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Legal Events

Date Code Title Description
MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ BY KZ KG MD TJ TM

MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): RU