EA200501144A1 - DEVICE AND METHOD FOR STORING PROTEINS - Google Patents

DEVICE AND METHOD FOR STORING PROTEINS

Info

Publication number
EA200501144A1
EA200501144A1 EA200501144A EA200501144A EA200501144A1 EA 200501144 A1 EA200501144 A1 EA 200501144A1 EA 200501144 A EA200501144 A EA 200501144A EA 200501144 A EA200501144 A EA 200501144A EA 200501144 A1 EA200501144 A1 EA 200501144A1
Authority
EA
Eurasian Patent Office
Prior art keywords
protein
storing
chamber
buffer solution
alkaline buffer
Prior art date
Application number
EA200501144A
Other languages
Russian (ru)
Other versions
EA008044B1 (en
Inventor
Дэвид Филип Кнайт
Лариса Пиннок
Original Assignee
Спин`Тек Инжиниринг Гмбх
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Спин`Тек Инжиниринг Гмбх filed Critical Спин`Тек Инжиниринг Гмбх
Publication of EA200501144A1 publication Critical patent/EA200501144A1/en
Publication of EA008044B1 publication Critical patent/EA008044B1/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/02General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
    • DTEXTILES; PAPER
    • D01NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01DMECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
    • D01D1/00Treatment of filament-forming or like material
    • D01D1/02Preparation of spinning solutions
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08LCOMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L89/00Compositions of proteins; Compositions of derivatives thereof
    • DTEXTILES; PAPER
    • D01NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01FCHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
    • D01F4/00Monocomponent artificial filaments or the like of proteins; Manufacture thereof
    • D01F4/02Monocomponent artificial filaments or the like of proteins; Manufacture thereof from fibroin

Abstract

В изобретении предлагается устройство для хранения протеина (20), которое содержит первую камеру (30) для хранения протеина (20) и вторую камеру (40) для хранения щелочного буферного раствора (50), причем вторая камера (40) имеет флюидальное сообщение с первой камерой (30). В соответствии с предпочтительным вариантом осуществления изобретения, щелочной буферный раствор содержит ионы кальция, которые способствуют загустеванию протеина (20). Предлагается также способ хранения протеина (20), который включает в себя следующие операции: первую операцию помещения протеина в первую камеру хранения (30); вторую операцию воздействия на протеин (20) при помощи щелочного буферного раствора (50); и третью операцию сохранения протеина (20) в щелочной среде в первой камере хранения (30).The invention proposes a device for storing protein (20), which contains a first chamber (30) for storing protein (20) and a second chamber (40) for storing an alkaline buffer solution (50), with the second chamber (40) having a fluid message with the first camera (30). In accordance with a preferred embodiment of the invention, the alkaline buffer solution contains calcium ions, which contribute to the thickening of the protein (20). A method of storing protein (20) is also proposed, which includes the following operations: the first operation of placing the protein in the first storage chamber (30); the second operation of exposure to protein (20) using an alkaline buffer solution (50); and the third operation of preserving protein (20) in an alkaline medium in the first storage chamber (30).

EA200501144A 2002-12-23 2003-12-23 Apparatus and method for storing proteins EA008044B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0230102.6A GB0230102D0 (en) 2002-12-23 2002-12-23 Apparatus and method for storing proteins
PCT/EP2003/014786 WO2004057068A1 (en) 2002-12-23 2003-12-23 Apparatus and method for storing proteins

Publications (2)

Publication Number Publication Date
EA200501144A1 true EA200501144A1 (en) 2006-04-28
EA008044B1 EA008044B1 (en) 2007-02-27

Family

ID=9950384

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200501144A EA008044B1 (en) 2002-12-23 2003-12-23 Apparatus and method for storing proteins

Country Status (15)

Country Link
US (1) US20060289327A1 (en)
EP (1) EP1576212A1 (en)
JP (1) JP2006511722A (en)
KR (1) KR20050084455A (en)
CN (1) CN1729319A (en)
AU (1) AU2003293981A1 (en)
BR (1) BR0317651A (en)
CA (1) CA2511183A1 (en)
EA (1) EA008044B1 (en)
GB (1) GB0230102D0 (en)
IL (1) IL169331A0 (en)
MX (1) MXPA05006953A (en)
NZ (1) NZ541236A (en)
WO (1) WO2004057068A1 (en)
ZA (1) ZA200505071B (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7148343B2 (en) * 2001-10-12 2006-12-12 Gentra Systems, Inc. Compositions and methods for using a solid support to purify RNA
WO2006052680A1 (en) 2004-11-05 2006-05-18 Qiagen North American Holdings, Inc. Compositions and methods for purifying nucleic acids from stabilization reagents
GB0807868D0 (en) * 2008-04-30 2008-06-04 Knight David P Cartilage repair material and a method for the preparation thereof
US20140378661A1 (en) * 2011-04-20 2014-12-25 Trustees Of Tufts College Molded regenerated silk geometries using temperature control and mechanical processing
JP2017170082A (en) * 2016-03-25 2017-09-28 優一郎 新崎 Brush bristle material and brush
CN109996805B (en) * 2016-11-29 2023-03-28 丝芭博株式会社 Protein composition, method for producing same, and method for improving thermal stability

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08295697A (en) * 1995-04-26 1996-11-12 Kanebo Ltd Production of aqueous solution of silk fibroin at high concentration
JP4194291B2 (en) * 2001-04-17 2008-12-10 興和株式会社 Process for producing undegraded silk fibroin aqueous solution and skin care agent containing the same
GB0126118D0 (en) * 2001-10-31 2002-01-02 Vollrath Friedrich W L Precursor feedstock for forming filaments

Also Published As

Publication number Publication date
MXPA05006953A (en) 2006-02-22
KR20050084455A (en) 2005-08-26
BR0317651A (en) 2005-12-06
WO2004057068A1 (en) 2004-07-08
CN1729319A (en) 2006-02-01
IL169331A0 (en) 2009-02-11
ZA200505071B (en) 2006-11-29
CA2511183A1 (en) 2004-07-08
EA008044B1 (en) 2007-02-27
AU2003293981A1 (en) 2004-07-14
NZ541236A (en) 2007-05-31
JP2006511722A (en) 2006-04-06
US20060289327A1 (en) 2006-12-28
EP1576212A1 (en) 2005-09-21
GB0230102D0 (en) 2003-01-29

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Legal Events

Date Code Title Description
MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ BY KZ KG MD TJ TM RU